Tissue culture and rapid propagation method of acer palmatum

A technology of tissue culture and Acer palmatum group, applied in horticultural methods, botany equipment and methods, cultivation, etc., can solve the problems that there is no research on Acer palmatum culture technology, achieve good differentiation of test-tube seedlings, increase reproduction speed, The effect of improving the survival rate of transplanting

Inactive Publication Date: 2010-10-06
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] At present, there is no relevant report on the rese

Method used

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Embodiment Construction

[0012] 1. Material handling

[0013] On a sunny morning, select plants from the field that grow vigorously, have no pests and diseases, and have better leaf color observed in the previous year, and take young shoots as explants. Cut the explants and remove the leaves (leaving only the top 1-2 leaves) to prevent the branches from losing water. The explants were carefully scrubbed with a soft brush dipped in detergent, then rinsed with tap water for 30 minutes, and set aside.

[0014] 2. Inoculation of the first generation

[0015] On the ultra-clean bench in the sterile room, treat with 70% alcohol for 30s, and then use 0.1% HgCl 2 Disinfect for 25 minutes, and finally rinse with sterile water 5 to 6 times. Cut the explants into 1.0-1.5cm-long stems with buds, blot the surface water and inoculate on the starter medium (NN69+NAA 0.1mg / L+TDZ0.3mg / L+sucrose 30g / L), and inoculate each bottle an explant. The temperature of the culture room is (24±1)°C, the light time is 12h / d, ...

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Abstract

The invention relates to a tissue culture and rapid propagation method of acer palmatum. The method comprises the following steps of: after a series of sterilization processes, carrying out isolated culture on acer palmatum stems by adopting NN69 as a basic culture medium; and establishing a rapid propagation system of the acer palmatum by utilizing hormone regulation and control to successfully culture an integrated rooting regeneration plant with a transplant survival rate of about 90 percent, wherein a starting culture medium for inoculating a primary generation comprises the NN69, 0.1mg/L of NAA (Naphthylacetic Acid), 0.3mg/L of TDZ (Thidiazuron) and 30g/L of sucrose with a pH value of 5.8; an enrichment culture medium comprises the NN69, 30g/L of sucrose, 0.1mg/L of NAA and 0.8mg/L of KT-30; and a rooting culture medium comprises 1/2 NN69, 0.2mg/L of NAA, 20g/L of sucrose and 0.2g/L of active carbon. The method overcomes the defects of long conventional seedling culturing period of the acer palmatum, low cuttage propagation coefficient, and the like and realizes the industrial production of acer palmatum nursery stocks.

Description

1. Technical field [0001] The object of the invention relates to the cultivation technology of plant seedlings, which is a rapid propagation method of Acer palmatum by tissue culture. 2. Background technology [0002] Acer palmatum (Acer.palmatum Thunb.) is a small deciduous tree or tree of the genus Acer in the family Aceraceae, with an umbrella-shaped crown and elegant posture. There are many varieties of leaf shapes and colors, including palmate and pinnate splits, red, purple and yellow, etc. It has strong resistance to sulfur dioxide and smoke and dust, and is more shade-tolerant. It grows well under the shade of tall trees. Acer palmatum is a tree species that is difficult to root, and the survival rate of cuttings is low. We have realized the factory production of Acer palmatum seedlings by using tissue culture technology. [0003] At present, there is no related report on tissue culture technology of Acer palmatum in China. 3. Contents of the invention [0004] ...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
Inventor 李倩中刘晓宏苏家乐李淑顺唐玲项立平
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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