34 results about "Hormone regulation" patented technology

The invention provides a strain of lactobacillus plantarum YS-AG23 with high antioxidant activity, and a rhizoma polygonati and radix angelicae sinensis fermented product obtained by fermenting rhizoma polygonati and radix angelicae sinensis with the strain of lactobacillus plantarum YS-AG23. In a D-galactose induced premature ovarian failure mouse model, the fermentation product can regulate up the levels of antioxidant and stress protection genes SOD2 and HO-1 and an anti-aging gene SIRT1 by regulating down the expression of a key gene AGER of an inflammation and oxidative stress pathway, so that the oxidative stress and inflammatory response of model mouse ovarian tissues are remarkably reduced; meanwhile, the serum hormone level can be specifically adjusted, the serum estradiol (E2) level can be increased, the follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels can be reduced to be close to the normal range, the expression of anti-mullerian hormone (AMH) and hormone regulatory gene LHR of ovarian reserve markers can be up-regulated, the proliferative activity and estrogen secretion function of follicular granulosa cells can be improved, and the effect of preventing ovarian reserve is achieved. The wet weight of atrophic uterus and ovary of the model mouse is remarkably increased, uterine and ovary atrophy is effectively reversed, and the ovarian reserve function of the D-galactose induced premature ovarian failure model mouse is recovered. Besides, in an H2O2-induced human dermal fibroblast aging model, the yeast can improve the activity of aging cells in a concentration-dependent manner and reduce the level of reactive oxygen species (ROS) in the cells; in addition, by up-regulating the expression of a collagen synthesis key gene COL1A1 and down-regulating the expression of a cell senescence marker gene p16, skin cell senescence caused by oxidative stress can be effectively improved. In conclusion, the polygonatum sibiricum and angelica sinensis leavening can synchronously improve premature ovarian failure and skin cell senescence caused by oxidative stress by accurately regulating and controlling oxidative stress, inflammatory response and tissue function related genes, and has wide application potential.

The invention discloses an ovarian protection composition containing ergothioneine and a preparation method of the ovarian protection composition, and relates to the technical field of ovarian protection compositions. The ovary protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.1 to 0.5 part of the ergothioneine, 0.05 to 1 part of S-equol, 1 to 5 parts of reducing coenzyme Q10, 1 to 3 parts of vitamin E, 10 to 20 parts of chitosan oligosaccharide, 30 to 50 parts of microcrystalline cellulose, 3 to 8 parts of hydroxypropyl methyl cellulose, 0.5 to 2 parts of silicon dioxide and 0.5 to 2 parts of a stabilizer. By adopting the stabilizer, photooxidation and hydrolysis of sensitive components are inhibited through molecular action, and the long-term stability of the composition in a humid and hot environment is remarkably improved in combination with the physical barrier action of silicon dioxide. Through the antioxidant synergistic effect of the ergothioneine and the vitamin E, the hormone regulation effect of the S-equol and the energy metabolism improvement effect of the coenzyme Q10, a multi-layer ovarian function protection system is constructed, and the health state of ovaries is improved. Through a special process, the tablet can be rapidly disintegrated and slowly released in an intestinal targeted manner, burst release of active ingredients is avoided, and the absorption efficiency is improved.

The invention belongs to the technical field of gene editing, and discloses a construction method of a uterus specific conditional gene editing mouse based on a CRISPR-AsCas12a system. According to the invention, the efficient editing ability of AsCas12a is innovatively combined with the uterus tissue specific Pgr promoter. According to the invention, rapid and efficient single gene knockout, such as Ythdf1, Ythdf2 and Ythdf3, is realized in the uterus. The technology breaks through the limitation of long breeding period of the traditional Cre / loxP system, and tissue-specific knockout can be realized without homozygosis. The CRISPR / AsCas12a system provides an animal model for researching pregnancy-related diseases and hormone regulation mechanisms and developing a uterus targeted gene therapy. Reference is provided for exploring other gene editing model animals, and development of gene editing animal models in the veterinary field is broadened.

The invention relates to the technical field of flower flowering phase regulation and control, and discloses a regulator and a method for promoting secondary flowering of crape myrtle, the regulator comprises 50 parts by weight of benzylaminopurine, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition; or 18 parts by weight of benzylaminopurine, 18 parts by weight of gibberellin, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition. The regulator provided by the invention comprehensively acts on multiple aspects of cell division, dormancy breaking, hormone regulation, nutrition supply, stress resistance enhancement and the like to jointly promote secondary blooming of the crape myrtle, increase the blooming amount and prolong the blooming period, and by virtue of multiple aspects of effects of pruning, fertilization, insect killing, plant growth regulator regulation and the like, the yield of the crape myrtle is increased. Secondary blooming of the crape myrtle is promoted, the blooming rate, the blooming duration and the blooming amount of secondary blooming are increased, and the growth vigor of the crape myrtle in the next year is not affected.

The invention belongs to the field of animal model construction, and particularly relates to application of an NFATC2IP gene knockout mouse model. Experiments prove that after the NFATC2IP gene is knocked out, the mouse has the characteristic changes of ovarian weight reduction, volume reduction, estrus cycle disorder, fertility reduction, AMH reduction, FSH increase and the like. It is indicated that the NFATC2IP gene is a key factor for maintaining ovarian reserve and endocrine balance, and an NFATC2IP gene knockout mouse can be used for revealing the occurrence mechanism of ovarian dysfunction and can also be used for screening of novel ovarian protection drugs, research of a hormone regulation mechanism and discovery and verification of biomarkers.

The invention discloses an in-vitro regeneration method of ferula sinkiangensis and application of the in-vitro regeneration method. According to the method, the efficient in-vitro regeneration method for the ferula sinkiang from embryos to complete in-vitro regenerated plants is established through hormone regulation and optimization, so that the germination efficiency of the embryos is improved, the high callus induction rate and seedling rate of the ferula sinkiang are obtained, the in-vitro regeneration efficiency of the ferula sinkiang is greatly improved, and the in-vitro regeneration cost is reduced. The efficient in-vitro regeneration method of the ferula sinkiangensis not only lays a good foundation for variety improvement and biotechnology breeding of the ferula sinkiangensis, but also provides technical support for resource protection and recovery and industrial application of the ferula sinkiangensis.

The application of the autologous dendritic cell in preparation of the medicine for treating the nerve injury erectile dysfunction can well promote recovery of the erectile function, perform staged reinfusion, induce immune regulation, repair nerve and blood vessel functions and improve a sex hormone regulation pathway. And the autologous dendritic cells are derived from a user, and have the advantages of safety, effectiveness and convenience in use. The combined immune cell therapy is obviously superior to a single treatment path in efficacy, is also more suitable for chronic, non-structural, subclinical and other ED people which are difficult to intervene by traditional methods, and has stronger individual adaptation ability and long-term controllability at the same time. Compared with a traditional scheme, the cell therapy targeting is better, and the treatment effect is faster.

The invention discloses a composition for improving prostate health based on plant synergistic interaction and a preparation method and application thereof.The composition is prepared from, by weight, 10-20 parts of saw palmetto extract, 5-15 parts of lycopene microcapsules, 15-30 parts of pumpkin seed powder, 5-10 parts of green tea extract, 3-8 parts of curcumin microcapsules and 15-40 parts of auxiliary materials; the auxiliary materials comprise a filling agent, a disintegrating agent and a lubricating agent, the filling agent accounts for 50-70% of the total weight of the auxiliary materials, the disintegrating agent accounts for 20-35% of the total weight of the auxiliary materials, and the lubricating agent accounts for 5-15% of the total weight of the auxiliary materials. According to the present invention, the plant components in the specific ratio are subjected to precise compatibility, and the differentiation microcapsule technology and the process optimization are combined so as to achieve the hormone regulation-anti-oxidation-anti-inflammatory synergistic effect, and the effect is far better than the effect of the single component; the bioavailability of raw material components can be effectively improved, the problem of absorption of fat-soluble components is solved, and an efficient and safe innovative scheme is provided for prostate health maintenance.

The invention relates to the technical field of plant tissue culture, in particular to a medium-grain coffee embryonic callus induction method which comprises the following steps: selecting medium-grain coffee leaves as explants for surface sterilization; sequentially carrying out three culture stages, namely a dedifferentiation stage, an embryogenic induction stage and an embryogenic maintenance stage, on the sterilized explant; in the dedifferentiation stage, a first culture medium added with 2, 4-dichlorphenoxyacetic acid is adopted, and culture is performed under a first culture condition; in the embryogenic induction period, a second culture medium added with 2, 4-dichlorphenoxyacetic acid and 6-benzyladenine is adopted, and culture is performed under a second culture condition; in the embryogenic maintenance period, a third culture medium added with 1-naphthylacetic acid and kinetin is adopted, and culture is performed under a third culture condition; according to the method disclosed by the invention, the compact embryonic callus with low waterlogging degree is obtained, and the induction rate of the embryonic callus reaches 85-95% through three-stage progressive hormone regulation and control, which is obviously higher than that of the prior art by 30-50%.

The present invention discloses a tissue culture and propagation method of Cyclamen using ovules as explants, belonging to the technical field of plant tissue culture. The method includes steps of ovule inoculation, callus induction and screening, bud induction and proliferation, strong seedling culture, rooting induction, and acclimatization and transplantation. By adopting methods such as using casein hydrolysate for nutritional support, dynamic hormone regulation, bud cluster proliferation, strong seedling state rooting, combination of in-vial acclimatization and ex-vial acclimatization, and improvement of transplantation substrate, the present invention provides an effective new way to improve the survival rate of Cyclamen tissue culture inoculation and seedling emergence.

The invention provides a rapid seedling raising method for pseudo-ginseng tissue culture, which comprises the following steps: (1) direct induction of explants: selecting leaves of pseudo-ginseng tissue culture seedlings or field seedlings as explants, tearing off petioles, lightly pressing wounds to enable the cuts to be in contact with the surface of a culture medium, carrying out culture by adopting a direct induction culture medium, skipping a callus stage, and taking the explants as explants; directly generating bud points; (2) anti-browning and hormone dynamic regulation and control: adding an explant incision protective agent into the direct induction culture medium, wherein the explant incision protective agent comprises activated carbon, polyvinylpyrrolidone and a silicon element concentrated solution; regulating and controlling the hormone proportion in stages; in the primary induction stage, adding salicylic acid and naphthylacetic acid; in the secondary induction stage, single naphthylacetic acid is adjusted; in a rooting stage, indolebutyric acid and kinetin are combined. The method mainly solves the problems of long period and high browning rate in panax notoginseng tissue culture.

The invention discloses a combined medicine for treating polycystic ovarian syndrome and application of the combined medicine. The combined medicine contains Ins (inositol and chiral inositol) and BBR (berberine). The invention proves that the combination of inositol, chiral inositol and berberine is superior to the combination of NAC (N-acetylcysteine), Ins and BBR in the aspects of improving hormone level, relieving obesity symptoms, improving fertility quality and increasing mitochondrial quality. And each technical index is obviously different from that of the PCOS group. The medicine effect is illuminated from the aspects of hormone regulation, metabolic capability, mitochondrial quality and fertility, the obvious treatment effect is achieved, and the result is more credible.

The invention belongs to a biopharmacy technology, and particularly relates to a fusion polypeptide and a derivative thereof, and an application of the fusion polypeptide and the derivative in preparation of medicines for treating alopecia, acne, dermatitis and other related diseases. The fusion polypeptide is formed by sequentially connecting an O peptide or a derivative peptide of the O peptide, an I peptide or a derivative peptide of the I peptide and two structural functional domains through a connecting peptide linker or directly connecting the two structural functional domains from the N end to the C end, or the fusion polypeptide is formed by directly connecting the peptide I or the derivative peptide thereof, the peptide O or the derivative peptide thereof and two structural functional domains through a connecting peptide linker in sequence from the N end to the C end; the fusion polypeptide provided by the invention can simultaneously activate GPR158 and / or GPRC6A and / or GPR37 pathways and simultaneously activate an integrin receptor pathway, has the remarkable advantages of high hair growth promotion activity, quick response and long hormone regulation action duration, and provides a new medicine for treating alopecia, acne, dermatitis and other related diseases.

The invention relates to the technical field of plant culture, in particular to a water culture method for promoting growth of radix ranunculi ternati roots. Comprising the following steps: preparing a radix ranunculi ternati hydroponic solution; and performing water culture on the radix ranunculi ternati root blocks. According to the invention, bergamot fruit enzyme, glycine and iron ions in ferric sulfate are complexed, glycine as a micromolecular organic ligand can form a stable soluble complex with the iron ions, precipitation of the iron ions in a hydroponic solution is avoided, a complex such as glycine iron is formed, and meanwhile, the content of glycine iron in the hydroponic solution is reduced. Organic matters degraded by the enzyme and iron glycinate form an organic-inorganic composite nutrition system, and antioxidant components of the enzyme can relieve oxidative damage to the root system and balance oxidative stress when iron is excessive, so that the absorption efficiency of the plant root system to the iron glycinate is improved, energy consumption is reduced, and the yield of the plant is increased. According to the combination, through organic-inorganic synergism, enzymatic activation and hormone regulation and control, the biomass accumulation and nutrient absorption efficiency of the radix ranunculi ternati root blocks is remarkably improved, so that the effect of promoting the growth of the radix ranunculi ternati root blocks is achieved.

The invention relates to the technical field of plant tissue culture, and is a method and application of rapid propagation of daqing tissue culture. The invention uses unlignified young stem segments as explants, sterilizes them with 75% alcohol and 0.1% mercuric chloride solution in sequence after cleaning, and establishes a rapid propagation technology system of daqing tissue culture through primary induction, secondary proliferation, strong bud culture and rooting culture, providing technical support for its production and reproduction, collection and preservation of wild germplasm resources, introduction and domestication, development and utilization. The invention controls the explant inoculation contamination rate within 15% and the mortality rate within 10% by controlling the explant collection time and sterilization time; the addition of polyvinyl pyrrolidone (PVP) during primary induction can effectively inhibit the browning of the explant; the primary induction rate is as high as 80% or more, the proliferation coefficient reaches about 11.6, and the rooting rate reaches more than 95% through hormone regulation; a certain concentration of coconut juice is added during strong bud and rooting culture to make the leaves more vivid green, the seedlings and roots more robust, and finally improve the survival rate of seedling hardening.

The invention belongs to the technical field of plant nutrient solutions. The invention provides a nutrient solution for promoting sympodial bamboos to root. The nutrient solution is prepared from the following components in parts by weight: 1 to 2 parts of major elements, 0.4 to 0.8 part of trace elements, 0.001 to 0.003 part of amino acid, 0.002 to 0.004 part of plant growth hormone, 0.2 to 0.5 part of saccharides, 0.1 to 0.2 part of vitamins, 0.0015 to 0.002 part of an osmotic regulator, 0.3 to 0.4 part of chitosan, 0.08 to 0.12 part of polyacrylamide, 0.001 to 0.003 part of a nano material and 10 to 30 parts of water. The nutrient solution improves the rooting efficiency and survival rate of sympodial bamboos through a composite system of nutrition supply, hormone regulation, structure support and permeation protection, and has good development and strong stress resistance and adaptability in growth height, leaf number, root length and the like. And the uniformity and the stability of the nutrient solution can be ensured by a segmented dissolution mode.

This invention discloses an Equoluxe multivitamin tablet containing a natural hormone regulator and its preparation process, belonging to the field of pharmaceutical technology. The invention comprises the following formula in parts by weight: 80-100 parts soybean extract, 20-55 parts plant-based lactic acid bacteria, 5-10 parts ginseng extract, 80 parts ginger extract, and 10-30 parts excipients. This invention, through a specific combination, works synergistically to promote estrogen secretion in women, enhance ovarian function, and maintain hormonal balance. By microencapsulating the plant-based lactic acid bacteria during pretreatment, this invention significantly improves the activity retention rate during tableting and storage, achieving an activity retention rate of over 87% after 6 months under accelerated conditions, thus solving the technical problem of poor stability in probiotic products. This invention employs a gradient mixing and low-temperature pressing process to avoid local aggregation of highly active ingredients and high-temperature inactivation, ensuring the uniformity of content and bioavailability of key components such as equoluxone and lactic acid bacteria in the tablets.

The invention relates to an application of urolithin A in preparation of a product for improving an endogenous estrogen level. The molecular formula of the urolithin A is C13H8O4, and the structural formula of the urolithin A is shown as a formula (I). The improvement of the endogenous estrogen level comprises the following steps: selectively regulating and controlling synthesis pathways of adrenal hormone and ovarian estrogen, and synergistically improving the in-vivo estrogen level. Besides, the urolithin A has good hormone regulation selectivity, can effectively inhibit cortisol secretion, and has no obvious influence on aldosterone level, so that blood pressure regulation and body fluid homeostasis are prevented from being interfered. The action characteristics lay a foundation for the application of the urolithin A in improvement of female climacteric hormone disorder and related health support directions, and the urolithin A has a wide prospect as an endogenous hormone regulation strategy. (I)

The invention relates to the technical field of functional food, and particularly discloses composite dietary therapy powder for improving intestinal motility disorder during pregnancy and a preparation method thereof, and the composite dietary therapy powder comprises the following components: composite nutrient fibers, cucumber seeds, sweet almonds, black sesames, red dates, banana composite slices, carrots, crystal sugar and a nutritional microbial agent; wherein the added composite nutrient fibers are subjected to enzymolysis through raw materials such as lily bulbs and the like, and then standing fermentation is performed, so that macromolecules and anti-nutrients can be decomposed, and the content of active ingredients is increased; the added nutritional microbial agent is decomposed through oligosaccharide and casein to form a load system, the strain is pre-activated to improve the activity and increase the content of active substances such as exopolysaccharides, the proliferation activity of probiotics is high, and the intestinal health is improved; the added banana composite slices decompose tannic acid through a process, and the content of oligosaccharide and other substances is increased; according to the invention, multiple nutritional ingredients take effect synergistically, the proportion of the ingredients is regulated and controlled, and the intestinal motility disorder of pregnant women during pregnancy is comprehensively improved from the aspects of hormone regulation, flora regulation, nutrition supplement and the like.

The invention discloses a ratooning rice narrow-row hormone regulation and control optimization method and system, particularly relates to the technical field of rice cultivation, and aims to solve the problem of yield loss caused by asynchronous germination of wide-row and narrow-row regenerated buds in the prior art. The method comprises the following steps: measuring an anaerobic index of narrow-row rhizosphere soil, and screening microbial active substances containing ACC deaminase when the anaerobic index exceeds a threshold value; secondly, analyzing absorption characteristics of regenerated buds through near infrared spectroscopy to realize dormancy depth grading; synchronously tracking a transmission time sequence of ethylene concentration from a root system to a bud to determine an inhibition window period; dynamically adjusting the compounding proportion of gibberellin GA3, cytokinin 6-BA and microbial active substances based on dormancy grading and a time window; and finally, directionally spraying the compound solution to the narrow-row area in the time window. By eliminating rhizosphere ethylene accumulation and accurately matching the physiological state and action time sequence of buds, the narrow-row regeneration bud germination efficiency and wide-row synchronization are realized.

The invention relates to the technical field of plant tissue culture, in particular to a medium-grain coffee embryonic callus induction method which comprises the following steps: selecting medium-grain coffee leaves as explants for surface sterilization; sequentially carrying out three culture stages, namely a dedifferentiation stage, an embryogenic induction stage and an embryogenic maintenance stage, on the sterilized explant; in the dedifferentiation stage, a first culture medium added with 2, 4-dichlorphenoxyacetic acid is adopted, and culture is performed under a first culture condition; in the embryogenic induction period, a second culture medium added with 2, 4-dichlorphenoxyacetic acid and 6-benzyladenine is adopted, and culture is performed under a second culture condition; in the embryogenic maintenance period, a third culture medium added with 1-naphthylacetic acid and kinetin is adopted, and culture is performed under a third culture condition; according to the method disclosed by the invention, the compact embryonic callus with low waterlogging degree is obtained, and the induction rate of the embryonic callus reaches 85-95% through three-stage progressive hormone regulation and control, which is obviously higher than that of the prior art by 30-50%.

The invention relates to the technical field of livestock breeding gene traceability positioning data analysis, and discloses a yak breeding excellent gene traceability positioning method and system based on deep learning, and the method comprises the steps: obtaining a six-dimensional environment stress factor time sequence data set and a five-dimensional breeding physiological stress response index set; a breeding hormone response model is constructed, and a prediction result is generated; dynamically adjusting an injection parameter set of gonadotropin release hormone and cloprostenol in the estrus synchronization treatment scheme, and forming and executing an individualized hormone regulation instruction; in a preset time range, performing artificial insemination by measuring blood flow parameters of the uterine artery of the yak in combination with the prediction result; a calf biological sample is collected, genome desoxyribonucleic acid is extracted, candidate gene locus typing detection is carried out, and an excellent gene traceability positioning analysis report is output. According to the invention, the integration of environmental adaptability, stress state monitoring and breeding technology precision cooperation is realized, and the breeding efficiency of yaks in a remote grazing mode is effectively optimized.

The invention discloses a culture medium for increasing anthocyanin content and a culture method. Relates to the technical field of tissue culture. The culture medium is prepared from the following raw materials based on a B5 culture medium: konjaku flour, 6-BA, NAA, cane sugar, glucose and salicylic acid. Accumulation of anthocyanin is promoted through hormone regulation, sugar signals and stress induction by improving a culture medium formula; through variable-temperature culture, temperature difference change is used for accumulation of anthocyanin of psammosilene tunicoides for the first time, and results show that the psammosilene tunicoides is beneficial to accumulation of secondary metabolites under the stress of a variable-temperature environment.

The application discloses a method for inducing callus of tender stems of red-awn sorghum, comprising the following steps: S1, taking red-awn sorghum seeds, inoculating the seeds in a seed culture medium after surface sterilization, and obtaining aseptic seedlings through illumination culture, and taking tender stems as explants; and S2, cutting the tender stems, inoculating the tender stems in a callus induction culture medium for dark culture until callus is formed; the callus induction culture medium takes MS as a basic culture medium and contains sucrose, agar, glycine, 2,4D, KT and citric acid. Through the addition of citric acid and hormone regulation, the method effectively inhibits the browning of the tender stem explants, improves the callus induction rate, and obtains high-quality callus with vigorous growth and dense texture, thereby providing technical support for genetic transformation and breeding of red-awn sorghum.

The invention belongs to the field of animal breeding, and particularly relates to a traditional Chinese and Tibetan medicine composition for improving the oestrus rate of yaks. The technical problems that the yak is low in oestrus rate and difficult to regulate and control, and hormone regulation and control are large in side effect are solved. According to the specific technical scheme, the pharmaceutical composition comprises gymnadenia conopsea, herba epimedii, angelica sinensis, leonurus, hucho taimen, fructus lycii, prepared rehmannia root, ligusticum wallichii, bighead atractylodes rhizome, mango kernel, fructus chebulae and rhizoma acori calami. According to the prescription provided by the invention, through reasonable compatibility of monarch, minister, assistant and guide, the reproductive system of the yak is adjusted from multiple aspects, the renal function is enhanced, the blood circulation of reproductive organs is improved, nutrient absorption is promoted, and the overall health state is enhanced, so that the aim of promoting oestrus and hybridization of the yak is achieved.

The invention provides a composite soaking agent for promoting synchronous seedling emergence of Chinese yam tubers as well as a preparation method and application of the composite soaking agent. The composite soaking agent is prepared from the following raw materials in parts by weight: 0.05 to 0.2 part of melatonin nano-liposome, 0.5 to 2.0 parts of sodium lignin sulfonate, 2 to 5 parts of garlic extracting solution, 5 to 10 parts of abscisic acid degrading enzyme activating agent, 3 to 8 parts of mitochondria activating agent, 2 to 6 parts of pyruvate kinase activating agent and 800 to 900 parts of purified water. The compound soaking agent solves the heterogeneity problem of tuber germination from multiple aspects through a five-in-one synergistic network of hormone regulation, energy enhancement, metabolism activation, oxidation resistance and bacterium resistance, and promotes tuber full seedling, so that integrated management of water, fertilizer and illumination in the later period is facilitated, the yield is increased, and the economic benefit is increased. A technical solution of a multi-target and multi-component synergistic mechanism is provided for high-quality and high-yield Chinese yam, and the method has remarkable application and popularization values.

The invention provides a method for regulating and inducing conception of non-pregnant non-oestrus dairy cows. The method is characterized by comprising the following steps: 1) detecting LH and FSH levels and performing staging; 2) carrying out regulation and targeted induction on cows with different LH and FSH stage levels; and 3) artificial insemination. Targeted treatment and precise regulation and control are achieved for high-difficulty breeding cases of 37 sky huai, no oestrus, lasting corpus luteum and uterus residues. The prostaglandin PGF2a + oxytocin + gonadotropin releasing hormone GnRH combined intramuscular injection scheme is adopted, lasting corpus luteum is thoroughly dissolved, residual embryonic tissues in the body are removed, the uterus is purified, the subsequent conception environment quality is remarkably improved, ultrasonic monitoring does not need to be frequently used, individualized and refined hormone regulation is achieved, the conception rate is remarkably increased, and meanwhile the conception time is shortened. The one-birth double-calf rate is stably improved, and the breeding economic benefits are greatly improved.

According to the self-adaptive production control unit based on the hormone regulation and control mechanism, the overstock task variable quantity and the product-in-process fluctuation quantity are introduced into controller parameter design, correlation between controller parameters and workshop system performance indexes is achieved, and the robustness of a control system is improved. When the control unit is in a conventional state, compared with a traditional controller, the designed controller is better in system stability. The production capacity can be adjusted in time, overstocked tasks can be rapidly eliminated, and meanwhile in the whole production capacity adjusting process, the product stock can be kept within a reasonable fluctuation range. In a limit state, when a product stock reaches a certain limit value, the damping characteristic of a workshop manufacturing system is limited to a certain extent. The backlog task controller based on the hormone regulation and control mechanism can regulate and control the production process more stably, and the production process is prevented from being excessively fluctuated. The value of the key parameter-production advance period of the system is changed, the system sensitivity is low, and the system requirements under different working conditions can be met.

The invention belongs to the technical field of plant preservation, and particularly relates to a compound preservative for lycoris plant fresh cut flowers as well as a preparation method and application of the compound preservative. The compound preservative is prepared from the following effective components in mass concentration: 150 to 200mg / L of citric acid, 15 to 20g / L of cane sugar, 2 to 3g / L of calcium chloride, 1 to 2g / L of chitosan, 1 to 2ppm of nano-silver colloid and 3 to 5mg / L of gibberellin. According to the invention, the nano-silver colloid, the chitosan and the hormone are combined for post-harvest treatment of lycoris plant cut flowers for the first time; four functions of antibiosis, cell energy stabilization, nutrition supply and hormone regulation are integrated, a multi-factor synergistic mechanism is established, and then the postharvest preservation effect of the lycoris plant cut flowers is remarkably improved. Results of the embodiment show that the composite preservative can prolong the life of the lycoris plant cut flower vase to 6-8 days, the phenomena that the base part of the flower stem is curled and the petals are premature senility are obviously reduced, and the good ornamental value is maintained.

The invention discloses a rosaceae plant embryonic stem cell acquisition method based on an ultrasonic synergistic hormone regulation system, and belongs to the technical field of plant stem cells. According to the method, rosaceae young fruits (such as apples and strawberries) with the development period of 30-50 days are used as explants, the pollution rate is reduced to be smaller than or equal to 5% through gradient disinfection (detergent-ethanol-sodium hypochlorite), embryo tissue is broken through 20kHz pulse ultrasonic waves (working for 1 second / intermittent for 5 seconds), and the tissue is subjected to tissue culture. Efficient separation and amplification of the stem cells are realized by combining a specific hormone proportioning culture medium; an induction culture medium (MS + 0.1-0.5 mg / L of GA3 + 0.1-0.5 mg / L of KT + 0.2 mg / L of cephalosporin) activates the activity of the stem cells to 88.5 + / -3.2%; the proliferation culture medium (MS + 0.5-1.0 mg / L 6-BA + 0.1-0.2 mg / L NAA + 0.1-0.5 mg / L KT + 0.2 mg / L cephalosporin) enables the cell density to reach 1.85 * 10 cells / mL, and the cell density is increased by 230% compared with that of a traditional method. The method breaks through the limitation that a traditional enzymolysis method is low in efficiency and high in damage, the high-purity embryonic stem cells can be obtained within 7 days, and an efficient cell platform is provided for development of medicinal components of the rosaceae and variety improvement.