45 results about "Casein hydrolysate" patented technology

The invention belongs to the technical field of health-care products, and particularly relates to a formula helpful for improving sleep and a preparation method thereof. Comprising the following raw material components in parts by weight: 90-110 parts of spina date seed powder, 45-55 parts of casein hydrolysate peptide powder, 15-22 parts of gamma-aminobutyric acid, 18-22 parts of lucid ganoderma powder, 15-22 parts of lily powder, 18-20 parts of poria cocos powder, 12-15 parts of tea leaf theanine and 1-4 parts of lactobacillus plantarum JYLP-326. According to the formula for improving sleep provided by the invention, the limitation of an existing insomnia treatment means is broken through through a three-dimensional synergistic network of traditional Chinese medicines, probiotics and functional components, and the formula realizes systematic improvement on insomnia core symptoms through multi-channel synergy of nerve regulation, intestinal brain axis intervention and immune balance, namely rapid induction of sleep.

The invention discloses an improved self-induction culture medium and a culture process, and belongs to the technical field of bioengineering. The improved self-induction culture medium is prepared from 10 g / L of glucose, 3-5 g / L of glycerin, 3 g / L of lactose, 24 g / L of yeast extract, 8 g / L of casein hydrolysate, 0.02-0.05 mM of malonic acid, 1 mM of methionine and 1-3 mM of MgSO4, a phosphate buffer solution is added to control the pH to be 7.2, and the improved self-induction culture medium is prepared by mixing after sterilization. Cells are inoculated to an improved self-induction culture medium for culture, automatic switching of growth-induction stages can be realized based on a pH and DO linkage dynamic feeding control method, the operation complexity is reduced, and the controllability is higher. The culture medium does not need a special inducer, cost is reduced, and protein expression quantity is improved.

The invention discloses a novel ginseng stem cell culture system which comprises a basic culture medium and a growth regulation composition, and the basic culture medium comprises ammonium sulfate, monopotassium phosphate, magnesium sulfate heptahydrate and calcium chloride; the growth regulating composition comprises 2, 4-dichlorphenoxyacetic acid, zeatin, salicylic acid and natural polysaccharide. The basic culture medium also comprises casein hydrolysate, coconut water extract and cane sugar. The composition also comprises an anti-oxidation composition. The invention further discloses a disposable flexible bioreactor for ginseng stem cell culture, the bioreactor comprises a tank body made of a flexible material, an air distribution device, a sterile sampling device and an online parameter monitoring device, and the top of the tank body structure is a cylindrical body and is provided with an inoculation opening, a culture medium liquid supplementing opening, an air outlet and a sampling opening; the bottom is an inverted cone provided with a parameter monitoring device interface and an air inlet, and the air inlet is connected with a ventilation device and an air flow adjusting device. The invention also discloses application of the culture system and the bioreactor.

The invention relates to a casein hydrolysate for the treatment of Crohn's disease. The composition of the casein hydrolysate is determined by the use of a proline- specific endoprotease for the hydrolysis of casein and is characterized in that the molar fraction of peptides of said hydrolysate carrying a carboxy terminal proline, expressed in %, is more than two times the molar fraction of proline, expressed in %, in the casein substrate used to generate the hydrolysate.

The present invention relates to a culture medium composition for producing botulinum toxin, and more particularly, to a culture medium composition for culturing Clostridium species capable of producing botulinum toxin. The culture medium composition of the present invention comprises casein hydrolysate and at least one plant-derived proteose peptone selected from the group consisting of pea hydrolysate, cottonseed hydrolysate, and wheat gluten hydrolysate. When Clostridium botulinum is cultured using the culture medium according to the present invention containing plant-derived proteose peptone, casein hydrolysate, and minerals, the growth rate of the bacteria in the culture medium is higher than that in each of the currently used culture medium and the culture medium containing only plant-derived proteose peptone. In addition, when the culture medium of the present invention is used, it is possible to produce a high concentration of botulinum toxin by culturing the bacteria in a safe manner.

The invention relates to a composition with an effect of improving sleep quality during pregnancy and application of the composition. The composition comprises aminobutyric acid, tea leaf theanine, casein hydrolysate and spearmint extract. The aminobutyric acid, the tea theanine, the casein hydrolysate and the spearmint extract are compounded for use, so that the problem that a single-mechanism sleep-aiding product has a limited effect on insomnia during pregnancy is solved, the sleep structure is improved to a great extent, and the sleep quality is improved.

The application provides a muscle building and shaping, fat control composition, a preparation method, application and product, and relates to the field of food technology.The composition according to the application is composed of the following raw materials in parts by weight: 30-100 parts of branched chain amino acid, 1-2 parts of casein hydrolysate, 10-50 parts of collagen peptide powder, 0.4-2 parts of cinnamon powder, 0.5-2 parts of purple tea concentrated powder, 5-10 parts of black pepper extract, 1-2 parts of concentrated freeze-dried watermelon powder and 2-8 parts of d-ribose; wherein the black pepper and ethanol solution are mixed, and the black pepper extract is obtained by means of extraction under the condition of heating and auxiliary microwave treatment. In the composition according to the application, the components interact with each other, and the muscle building, shaping and fat control effects are synergistically improved. The composition according to the application is applied to products with the effects of muscle building, shaping and fat control, and the effects are remarkable.

This invention relates to the field of food processing technology and discloses a process for preparing plant-based beverages containing casein hydrolysate peptides. The process includes: preheating a base liquid containing casein hydrolysate peptides and anionic polysaccharides and pumping it into a Venturi channel; inducing polysaccharide volume expansion by utilizing the hydrostatic pressure drop in the venturi channel to construct an isolation layer; simultaneously aspirating a low-temperature plant polyphenol extract, using the temperature difference to form an adhesion interface on the surface of the isolation layer; after the mixed fluid enters the expansion section, using the compressive force generated by hydrostatic recovery to press the polyphenols into the polysaccharide gaps, constructing a composite physical isolation phase; and triggering polysaccharide thermal gelation crosslinking by heating to establish a physical barrier. This invention utilizes the microscopic encapsulation mechanism generated by the fluid pressure field and temperature gradient to effectively block contact between polyphenols and protein molecules, inhibit complexation precipitation under thermal shock, and ensure that the beverage system possesses excellent thermal stability and storage stability.

The application provides a lacto-casein hydrolysate with anti-inflammatory effect and a preparation method and application thereof.The lacto-casein hydrolysate of the application comprises a peptide segment FAWPQYLK, a peptide segment LPW, a peptide segment MPLW and a peptide segment PLW, can reduce the release of pro-inflammatory cytokines, such as IL-1beta, IL-2, IL-6 and TNF-alpha, and improve the release of anti-inflammatory cytokines, such as IL-10, so as to reduce the cell inflammatory response, and further achieve the anti-inflammatory effect.

The invention provides a composition for promoting protein peptide absorption by utilizing molecular association and application of the composition to deep sleep promotion. The composition is prepared from a passion flower extract, casein hydrolysate, gamma-aminobutyric acid, collagen tripeptide, associated molecules and vitamin B complex. According to the composition, absorption of protein peptides is promoted through molecular association, gastrointestinal tract absorption disorders are reduced, all the components in the composition supplement one another and interact with one another, and the effect of promoting deep sleep can be effectively achieved. The oral preparation described in the invention can be a drug loading system such as a capsule, a tablet, a granule and the like.

The present invention discloses a tissue culture and propagation method of Cyclamen using ovules as explants, belonging to the technical field of plant tissue culture. The method includes steps of ovule inoculation, callus induction and screening, bud induction and proliferation, strong seedling culture, rooting induction, and acclimatization and transplantation. By adopting methods such as using casein hydrolysate for nutritional support, dynamic hormone regulation, bud cluster proliferation, strong seedling state rooting, combination of in-vial acclimatization and ex-vial acclimatization, and improvement of transplantation substrate, the present invention provides an effective new way to improve the survival rate of Cyclamen tissue culture inoculation and seedling emergence.

The invention relates to a construction method of an Agrobacterium rhizogenes mediated acacia melanoxylon genetic transformation system, which comprises the following steps: by taking an acacia melanoxylon SR17 sterile single plant as an explant, selecting an Agrobacterium rhizogenes strain Ar1193, infecting for 45 minutes in a heavy suspension of which the bacterial liquid concentration OD600 is equal to 0.9 and the acetosyringone concentration is 100 [mu] mol.L <-1 >, co-culturing for 3 days, and then transferring into a bacteriostatic culture medium to induce hairy roots; and taking the transgenic hairy roots obtained by induction as explants, and inoculating the explants into a WPM culture medium containing 0.05 mg.L <-1 > TDZ and 200 mg.L <-1 > casein hydrolysate to induce regeneration of adventitious buds so as to obtain complete transgenic plants. The screening concentrations of the four antibiotics, namely kanamycin, hygromycin, glyphosate and glufosinate-ammonium, are determined; a stable and efficient acacia melanoxylon genetic transformation system is established, the hairy root induction rate reaches 63.17%, the transgenic positive rate reaches 15.42%, and the adventitious bud regeneration rate reaches 52%.

The present invention discloses a fermentation medium for high-yield dextran production from Leuconostoc mesenteroides GFMO1. The medium for the growth of the bacteria is formulated from the sucrose agar medium, whereby the yield of the Exopolysaccharide produced is increased upon the addition of casein hydrolysate and bacteriological peptone in the fermentation medium. Casein acidic hydrolysate and bacteriological peptone are defined sources of amino acids that supplement organic growth factors such as vitamins. The main role of the defined source is to reduce the fermentation time of the bacteria, enhance viable numbers of lactic acid bacteria, and promote the production yield..

The invention discloses a litsea cubeba secondary embryo proliferation culture medium and a proliferation culture method, the culture medium is based on an MS culture medium, cane sugar, agar, casein hydrolysate, 6-benayl aminopurine, naphthylacetic acid, inositol and cyclodextrin are added, and the pH value is 5.6-5.8; the method comprises the following steps: a somatic embryo generation stage, a secondary embryo proliferation stage and regular removal of mature somatic embryos. According to the method, the immature seeds are adopted as explants, through the somatic embryogenesis culture medium and the secondary embryo proliferation culture medium of a specific formula, dark culture and periodic subculture are combined, the proliferation efficiency of the secondary embryos is remarkably improved, the highest proliferation multiple can reach 20.17 times, meanwhile, the endophyte pollution risk is reduced, and the genetic stability is guaranteed. The method is simple and convenient to operate, short in culture period, low in cost and suitable for large-scale industrial application, provides important technical support for rapid propagation, germplasm preservation and industrial development of litsea cubeba, and has remarkable economic and social benefits.

The invention provides a modified milk-derived active peptide loaded with probiotics and application of the modified milk-derived active peptide in a sleep-aiding milk beverage, polyethylene glycol is introduced into casein hydrolysate decapeptide YLGYLEQLLR for modification to prepare modified polypeptide, hydrogel is formed based on self-assembly of the modified polypeptide, and the probiotics are embedded to prepare the modified milk-derived active peptide loaded with the probiotics. The modified milk-derived active peptide can embed and slowly release the probiotics, so that the probiotics are continuously released in vivo, and the sleep quality is improved; in addition, the probiotics and the polypeptide synergistically play a role, so that the mood is regulated, and the sleep quality is improved.

The invention relates to the technical field of nutrition and health, in particular to a sleep-promoting composition containing oriental cherry polyphenol and a preparation method of the sleep-promoting composition. The oriental cherry polyphenol-containing sleep promoting composition is prepared from the following components in parts by mass: 5 to 8 parts of lemon myrtle leaf powder, 1 to 5 parts of oriental cherry polyphenol, 15 to 20 parts of casein hydrolyzed peptide, 15 to 25 parts of semen ziziphi spinosae powder, 5 to 8 parts of rhizoma gastrodiae powder, 8 to 12 parts of poria cocos powder, 2 to 10 parts of Borojo powder, 1 to 5 parts of N-acetylneuraminic acid, 10 to 15 parts of corn leaf extract and 0.5 to 5 parts of silicon dioxide. The lemon myrtle leaf powder, the sakura polyphenol, the casein hydrolyzed peptide and the N-acetylneuraminic acid are used as main effective components, and the obtained sakura polyphenol composition has the effects of improving sleep, resisting oxidation and improving the immunity of a human body and can be used as health-care food.

The present application provides a nutrient composition including: stachydrine, lactobacillus plantarum, walnut peptide, desert cistanche, casein hydrolysate, and two or more of theanine. The present inventors found that the above nutrient composition has a brain health function in the direction of anxiolytic, antioxidant, neuroprotective, and memory improvement.

The invention discloses a cardiac troponin T calibrator diluent composition, a diluent, a calibrator as well as a preparation method and application of the calibrator. The composition comprises a basic diluent component, a potassium salt, a casein hydrolysate and dodecyl trimethyl ammonium bromide, and the mass ratio of the potassium salt to the casein hydrolysate to the dodecyl trimethyl ammonium bromide is (5-39): (1-20): (0.5-3). According to the provided cardiac troponin T calibrator diluent composition and diluent, by optimizing the variety and concentration in the formula, the stability of cardiac troponin T in a liquid state and a freeze-drying state is remarkably improved, adsorption and degradation of cardiac troponin T in a glass container are effectively inhibited, the cardiac troponin T can be stabilized in a room-temperature glass bottle, and the application range of the cardiac troponin T calibrator diluent composition is widened. And after redissolution, good concentration consistency is still maintained, and reliable stability guarantee is provided for preparation, freeze-drying and clinical detection of the cardiac troponin T calibrator.

The present invention belongs to the technical field of plant propagation and cultivation, and in particular relates to a basal culture medium, a tissue culture medium, and a regeneration tissue culture method for regenerating long-barreled Lycoris radiata. The present invention provides a tissue culture medium for regenerating long-barreled Lycoris radiata, the tissue culture medium comprising an induction medium, a proliferation medium, a differentiation medium, a bud-strengthening medium, and a seedling-strengthening and rooting medium; in the tissue culture medium provided by the present invention, a modified MS medium and a modified B5 medium are used as the basal culture medium, and the combined action of 2,4-D, 6-BA, IBA, NAA, carbon nanotubes, and casein hydrolyzate facilitates the induction of long-barreled Lycoris radiata callus formation, proliferation, differentiation, and rooting, and sucrose, an energy substance, is combined to jointly improve the propagation coefficient of the long-barreled Lycoris radiata, thereby obtaining a large number of long-barreled Lycoris radiata tissue culture seedlings.

The present invention provides a method of preventing or treating inflammatory skin diseases by administering a composition comprising at least one milk-derived protein. The invention also relates to a method for moisturizing dry skin, softening skin, reducing skin itch, reducing skin desquamation, reducing skin redness, reducing rough skin, reducing skin swelling, reducing skin dehiscence, renewing skin cells, protecting hand skin or any combination thereof.

The invention discloses a casein source DPP-IV inhibitory peptide as well as a preparation method and application thereof. The casein source DPP-IV inhibition tetrapeptide and the casein source DPP-IV inhibition pentapeptide are MPIQ and MPIQA respectively, the DPP-IV inhibition activity is high, the IC50 value is 78.89-108.82 mu M, and the casein source DPP-IV inhibition tetrapeptide and the casein source DPP-IV inhibition pentapeptide can be prepared through a chemical synthesis method or obtained through biological enzymolysis of casein and can be used for preparing food or medicine with the blood sugar reducing effect. Besides, the casein hydrolysate containing the tetrapeptide MPIQ and the pentapeptide MPIQA is prepared through a biological enzymolysis method, the raw material cost is low, operation is convenient, and the casein hydrolysate also has high DPP-IV inhibitory activity.

The invention relates to casein peptide hydrolysate and application thereof in preparation of drugs for preventing or treating nerve aging, and belongs to the technical field of biological medicines. The casein hydrolysate is obtained by fermenting a casein solution with the probiotic bifidobacterium longum BNCC 185354, and the obtained casein hydrolysate has superoxide anion free radical scavenging capacity, can prolong the telomere length of the hippocampus of a subacute aging model mouse and relieve DNA oxidative damage of the hippocampus of the mouse. The brand-new casein peptide with both brain-accessible activity and biological activity is further screened from casein hydrolysate, free radicals can be removed, nerve cell inflammation can be eliminated, and therefore nerve aging is delayed. The casein peptide and the casein hydrolysate provided by the invention can be applied to preparation of drugs for preventing and / or treating nerve aging, and have good application prospects.

The invention discloses a composition for improving sleep and application thereof.The composition comprises chamomile powder, gamma-aminobutyric acid and casein hydrolysate peptide, any two of the chamomile powder, the gamma-aminobutyric acid and the casein hydrolysate peptide can be compounded according to a specific proportion, and the three of the chamomile powder, the gamma-aminobutyric acid and the casein hydrolysate peptide can also be compounded according to a specific proportion. When the three components are compounded, the composition comprises the following components in parts by mass: 0.1-10 parts of chamomile powder; 0.5 to 11 parts of gamma-aminobutyric acid; 0.5 to 10 parts of casein hydrolysate peptide; any two components are compounded according to a specific ratio and then are compounded with the third component. In the matching range of the application, the improvement effects of the composition compounded by the three components on the aspects of sleep round times, sleep duration and average sleep duration are basically close to those of the positive control melatonin, and the sleep improvement rate and the average sleep duration of the composition are superior to those of the positive control melatonin under some specific formulas.

The invention discloses a method for establishing a bee Parkinson's disease model based on characterization of dyskinesia and the like, which is characterized by comprising the following steps of: animal selection: selecting a mature bee as an object for establishing the bee Parkinson's disease model; preparing a modeling diet: preparing a sucrose solution containing casein hydrolysate and rotenone; and model establishment: feeding the mature bees with a modeling diet for 7 days to establish the bee Parkinson's disease model. The invention further discloses an application of the parkinson's disease model, established by the method, of the bacteria bees and / or the sterile bees. The method is easy and convenient to operate and low in cost, the motion distance and the motion speed of the built model bee are remarkably decreased, obvious dyskinesia is shown, and the survival rate of 5 days in an existing bee Parkinson model building technology is effectively increased to about 80% of 7 days from 5 days lower than 30%. The bee Parkinson's disease model established by the method can be used for screening new methods or new drugs for preventing or treating dyskinesia related diseases.

The invention relates to the technical field of preparation of bioactive peptides, in particular to casein hydrolysate capable of improving bifunctional activity and a preparation method of the casein hydrolysate. According to the research, casein is taken as a raw material, and the casein hydrolysate with high-activity alpha-amylase inhibitory activity and DPPH free radical scavenging rate is prepared through ultrasonic-assisted enzymolysis. Compared with a traditional casein peptide preparation method, the operation technology is simple, the whole technological process can meet the food-grade requirement, and the casein peptide preparation method can be applied to the fields of medicine, health care products, food and the like.

The invention provides hypoallergenic eye-protecting children milk tablets and a preparation method thereof, and the hypoallergenic eye-protecting children milk tablets comprise hypoallergenic formula whey protein powder, casein hydrolysate freeze-dried powder, eye-protecting composite active ingredients, a flavoring agent, an antioxidant and auxiliary materials, wherein the low-sensitization formula whey protein powder comprises a mixture of lactoferrin powder, alpha-lactalbumin powder and low-sensitization beta-LG protein powder, and the low-sensitization beta-LG protein powder comprises a non-covalent compound formed by combining a small molecule substance and cow milk beta-LG; the casein hydrolysate freeze-dried powder comprises bovine milk casein hydrolyzed by Alcalase protease and bacillus licheniformis protease. The selected small molecule substances are all dairy product available additives permitted by GB2760-2024 National Food Safety Standard Food Additives Usage Standards, and related reports of the food additives for beta-LG sensitization improvement do not exist in the existing research at present, so that the blank in the prior art is filled, and the eating safety of children is guaranteed.

The present invention provides a milk casein hydrolyzate and its preparation method and application. The composition of the milk casein hydrolyzate of the present invention includes at least one of the peptide segment 1 with an amino acid sequence as shown in SEQ ID NO: 1, the peptide segment 2 with an amino acid sequence as shown in SEQ ID NO: 2, and the peptide segment 3 with an amino acid sequence as shown in SEQ ID NO: 3. The milk casein hydrolyzate has good neuronal protection and repair effects, can improve cholinergic neuron and dopaminergic neuron damage; can inhibit Aβ protein deposition, thereby alleviating neuronal synaptic disorders, neuronal apoptosis and brain damage caused by Aβ protein deposition; can improve cholinergic defects, thereby alleviating neurodevelopmental impairment, behavioral and motor disorders caused by cholinergic defects; can also repair damaged neurons in the hippocampus and hypothalamus, thereby maintaining a stable brain environment.

The invention belongs to the technical field of polypeptide extraction, and relates to an anti-stress polypeptide composition and a preparation method thereof. A novel functional peptide group with a specific amino acid sequence combination (such as short peptides rich in tyrosine, proline and glutamine) is enriched from complex casein hydrolysate through an innovative separation strategy, the biological activity of the novel functional peptide group is remarkably superior to that of a traditional crude hydrolysate, and the novel functional peptide group belongs to a brand-new functional active substance for feed or veterinary medicine. Therefore, the traditional Chinese medicine composition not only can be used as an auxiliary treatment preparation in veterinary clinic for intervening stress, inflammatory bowel disease or immunosuppression and other diseases, but also can be used as a functional feed additive to be widely applied to daily ration of livestock and poultry and pets, the disease resistance and production performance of animals are improved, the market application prospect is wide, and the traditional Chinese medicine composition has important economic and social values.

The invention provides a preparation method and application of a potassium promoting type microbial fertilizer. The fertilizer is prepared by compounding paenibacillus mucilaginosus functional microspheres, activated bacterial powder and citric acid modified biochar. The inner core of the paenibacillus mucilaginosus functional microsphere is a diatomite-casein hydrolysate porous nutrition carrier loaded with thalli and provides physical support and endogenous nutrition, and the shell of the paenibacillus mucilaginosus functional microsphere is a calcium alginate gel layer and is embedded into a sinapic acid molecularly imprinted polymer microsphere; when root systems of target crops such as rape and leaf mustard secrete sinapic acid due to potassium deficiency, the molecules are specifically recognized and combined to trigger shell degradation, so that internal functional bacteria are released as required, the released bacteria activate soil mineral potassium, the modified charcoal adsorbs and stores potassium ions, and loss is reduced. Matching of microbial inoculum release and crop potassium demand signals is achieved, and the survival rate, the action pertinence and the potassium utilization efficiency of the microbial inoculum are improved.

A casein hydrolysate-chlorogenic acid non-covalent complex emulsion as well as a preparation method and application thereof belong to the technical field of food processing. In view of the fact that the prior art cannot simultaneously consider the alpha-glucosidase inhibitory activity, emulsification stability and antioxidant activity of the casein hydrolysate, the casein hydrolysate-chlorogenic acid complex is prepared through non-covalent interaction, and the complex is used as an emulsifier to construct a functional emulsion. The interaction mainly occurs through hydrogen bonds and hydrophobic interaction, resulting in the unfolding of protein molecular structure and the fluorescence quenching. Meanwhile, the functional emulsion has good emulsification activity and stability. The emulsion prepared by using the complex has the smallest particle size, the lowest turbidity and uniform droplet distribution. The functional emulsion obtained by the application has the antidiabetic effect and excellent emulsification performance, and has important application value in the development of functional foods.