30 results about "Casein hydrolysate" patented technology

The invention discloses a novel ginseng stem cell culture system which comprises a basic culture medium and a growth regulation composition, and the basic culture medium comprises ammonium sulfate, monopotassium phosphate, magnesium sulfate heptahydrate and calcium chloride; the growth regulating composition comprises 2, 4-dichlorphenoxyacetic acid, zeatin, salicylic acid and natural polysaccharide. The basic culture medium also comprises casein hydrolysate, coconut water extract and cane sugar. The composition also comprises an anti-oxidation composition. The invention further discloses a disposable flexible bioreactor for ginseng stem cell culture, the bioreactor comprises a tank body made of a flexible material, an air distribution device, a sterile sampling device and an online parameter monitoring device, and the top of the tank body structure is a cylindrical body and is provided with an inoculation opening, a culture medium liquid supplementing opening, an air outlet and a sampling opening; the bottom is an inverted cone provided with a parameter monitoring device interface and an air inlet, and the air inlet is connected with a ventilation device and an air flow adjusting device. The invention also discloses application of the culture system and the bioreactor.

The invention relates to a casein hydrolysate for the treatment of Crohn's disease. The composition of the casein hydrolysate is determined by the use of a proline- specific endoprotease for the hydrolysis of casein and is characterized in that the molar fraction of peptides of said hydrolysate carrying a carboxy terminal proline, expressed in %, is more than two times the molar fraction of proline, expressed in %, in the casein substrate used to generate the hydrolysate.

The invention relates to a composition with an effect of improving sleep quality during pregnancy and application of the composition. The composition comprises aminobutyric acid, tea leaf theanine, casein hydrolysate and spearmint extract. The aminobutyric acid, the tea theanine, the casein hydrolysate and the spearmint extract are compounded for use, so that the problem that a single-mechanism sleep-aiding product has a limited effect on insomnia during pregnancy is solved, the sleep structure is improved to a great extent, and the sleep quality is improved.

The application provides a muscle building and shaping, fat control composition, a preparation method, application and product, and relates to the field of food technology.The composition according to the application is composed of the following raw materials in parts by weight: 30-100 parts of branched chain amino acid, 1-2 parts of casein hydrolysate, 10-50 parts of collagen peptide powder, 0.4-2 parts of cinnamon powder, 0.5-2 parts of purple tea concentrated powder, 5-10 parts of black pepper extract, 1-2 parts of concentrated freeze-dried watermelon powder and 2-8 parts of d-ribose; wherein the black pepper and ethanol solution are mixed, and the black pepper extract is obtained by means of extraction under the condition of heating and auxiliary microwave treatment. In the composition according to the application, the components interact with each other, and the muscle building, shaping and fat control effects are synergistically improved. The composition according to the application is applied to products with the effects of muscle building, shaping and fat control, and the effects are remarkable.

This invention relates to the field of food processing technology and discloses a process for preparing plant-based beverages containing casein hydrolysate peptides. The process includes: preheating a base liquid containing casein hydrolysate peptides and anionic polysaccharides and pumping it into a Venturi channel; inducing polysaccharide volume expansion by utilizing the hydrostatic pressure drop in the venturi channel to construct an isolation layer; simultaneously aspirating a low-temperature plant polyphenol extract, using the temperature difference to form an adhesion interface on the surface of the isolation layer; after the mixed fluid enters the expansion section, using the compressive force generated by hydrostatic recovery to press the polyphenols into the polysaccharide gaps, constructing a composite physical isolation phase; and triggering polysaccharide thermal gelation crosslinking by heating to establish a physical barrier. This invention utilizes the microscopic encapsulation mechanism generated by the fluid pressure field and temperature gradient to effectively block contact between polyphenols and protein molecules, inhibit complexation precipitation under thermal shock, and ensure that the beverage system possesses excellent thermal stability and storage stability.

The application provides a lacto-casein hydrolysate with anti-inflammatory effect and a preparation method and application thereof.The lacto-casein hydrolysate of the application comprises a peptide segment FAWPQYLK, a peptide segment LPW, a peptide segment MPLW and a peptide segment PLW, can reduce the release of pro-inflammatory cytokines, such as IL-1beta, IL-2, IL-6 and TNF-alpha, and improve the release of anti-inflammatory cytokines, such as IL-10, so as to reduce the cell inflammatory response, and further achieve the anti-inflammatory effect.

The invention relates to a construction method of an Agrobacterium rhizogenes mediated acacia melanoxylon genetic transformation system, which comprises the following steps: by taking an acacia melanoxylon SR17 sterile single plant as an explant, selecting an Agrobacterium rhizogenes strain Ar1193, infecting for 45 minutes in a heavy suspension of which the bacterial liquid concentration OD600 is equal to 0.9 and the acetosyringone concentration is 100 [mu] mol.L <-1 >, co-culturing for 3 days, and then transferring into a bacteriostatic culture medium to induce hairy roots; and taking the transgenic hairy roots obtained by induction as explants, and inoculating the explants into a WPM culture medium containing 0.05 mg.L <-1 > TDZ and 200 mg.L <-1 > casein hydrolysate to induce regeneration of adventitious buds so as to obtain complete transgenic plants. The screening concentrations of the four antibiotics, namely kanamycin, hygromycin, glyphosate and glufosinate-ammonium, are determined; a stable and efficient acacia melanoxylon genetic transformation system is established, the hairy root induction rate reaches 63.17%, the transgenic positive rate reaches 15.42%, and the adventitious bud regeneration rate reaches 52%.

The present invention discloses a fermentation medium for high-yield dextran production from Leuconostoc mesenteroides GFMO1. The medium for the growth of the bacteria is formulated from the sucrose agar medium, whereby the yield of the Exopolysaccharide produced is increased upon the addition of casein hydrolysate and bacteriological peptone in the fermentation medium. Casein acidic hydrolysate and bacteriological peptone are defined sources of amino acids that supplement organic growth factors such as vitamins. The main role of the defined source is to reduce the fermentation time of the bacteria, enhance viable numbers of lactic acid bacteria, and promote the production yield..

The invention relates to the technical field of nutrition and health, in particular to a sleep-promoting composition containing oriental cherry polyphenol and a preparation method of the sleep-promoting composition. The oriental cherry polyphenol-containing sleep promoting composition is prepared from the following components in parts by mass: 5 to 8 parts of lemon myrtle leaf powder, 1 to 5 parts of oriental cherry polyphenol, 15 to 20 parts of casein hydrolyzed peptide, 15 to 25 parts of semen ziziphi spinosae powder, 5 to 8 parts of rhizoma gastrodiae powder, 8 to 12 parts of poria cocos powder, 2 to 10 parts of Borojo powder, 1 to 5 parts of N-acetylneuraminic acid, 10 to 15 parts of corn leaf extract and 0.5 to 5 parts of silicon dioxide. The lemon myrtle leaf powder, the sakura polyphenol, the casein hydrolyzed peptide and the N-acetylneuraminic acid are used as main effective components, and the obtained sakura polyphenol composition has the effects of improving sleep, resisting oxidation and improving the immunity of a human body and can be used as health-care food.

The present application provides a nutrient composition including: stachydrine, lactobacillus plantarum, walnut peptide, desert cistanche, casein hydrolysate, and two or more of theanine. The present inventors found that the above nutrient composition has a brain health function in the direction of anxiolytic, antioxidant, neuroprotective, and memory improvement.

The invention discloses a cardiac troponin T calibrator diluent composition, a diluent, a calibrator as well as a preparation method and application of the calibrator. The composition comprises a basic diluent component, a potassium salt, a casein hydrolysate and dodecyl trimethyl ammonium bromide, and the mass ratio of the potassium salt to the casein hydrolysate to the dodecyl trimethyl ammonium bromide is (5-39): (1-20): (0.5-3). According to the provided cardiac troponin T calibrator diluent composition and diluent, by optimizing the variety and concentration in the formula, the stability of cardiac troponin T in a liquid state and a freeze-drying state is remarkably improved, adsorption and degradation of cardiac troponin T in a glass container are effectively inhibited, the cardiac troponin T can be stabilized in a room-temperature glass bottle, and the application range of the cardiac troponin T calibrator diluent composition is widened. And after redissolution, good concentration consistency is still maintained, and reliable stability guarantee is provided for preparation, freeze-drying and clinical detection of the cardiac troponin T calibrator.

The present invention provides a method of preventing or treating inflammatory skin diseases by administering a composition comprising at least one milk-derived protein. The invention also relates to a method for moisturizing dry skin, softening skin, reducing skin itch, reducing skin desquamation, reducing skin redness, reducing rough skin, reducing skin swelling, reducing skin dehiscence, renewing skin cells, protecting hand skin or any combination thereof.

The invention relates to casein peptide hydrolysate and application thereof in preparation of drugs for preventing or treating nerve aging, and belongs to the technical field of biological medicines. The casein hydrolysate is obtained by fermenting a casein solution with the probiotic bifidobacterium longum BNCC 185354, and the obtained casein hydrolysate has superoxide anion free radical scavenging capacity, can prolong the telomere length of the hippocampus of a subacute aging model mouse and relieve DNA oxidative damage of the hippocampus of the mouse. The brand-new casein peptide with both brain-accessible activity and biological activity is further screened from casein hydrolysate, free radicals can be removed, nerve cell inflammation can be eliminated, and therefore nerve aging is delayed. The casein peptide and the casein hydrolysate provided by the invention can be applied to preparation of drugs for preventing and / or treating nerve aging, and have good application prospects.

The invention discloses a composition for improving sleep and application thereof.The composition comprises chamomile powder, gamma-aminobutyric acid and casein hydrolysate peptide, any two of the chamomile powder, the gamma-aminobutyric acid and the casein hydrolysate peptide can be compounded according to a specific proportion, and the three of the chamomile powder, the gamma-aminobutyric acid and the casein hydrolysate peptide can also be compounded according to a specific proportion. When the three components are compounded, the composition comprises the following components in parts by mass: 0.1-10 parts of chamomile powder; 0.5 to 11 parts of gamma-aminobutyric acid; 0.5 to 10 parts of casein hydrolysate peptide; any two components are compounded according to a specific ratio and then are compounded with the third component. In the matching range of the application, the improvement effects of the composition compounded by the three components on the aspects of sleep round times, sleep duration and average sleep duration are basically close to those of the positive control melatonin, and the sleep improvement rate and the average sleep duration of the composition are superior to those of the positive control melatonin under some specific formulas.

The invention discloses a method for establishing a bee Parkinson's disease model based on characterization of dyskinesia and the like, which is characterized by comprising the following steps of: animal selection: selecting a mature bee as an object for establishing the bee Parkinson's disease model; preparing a modeling diet: preparing a sucrose solution containing casein hydrolysate and rotenone; and model establishment: feeding the mature bees with a modeling diet for 7 days to establish the bee Parkinson's disease model. The invention further discloses an application of the parkinson's disease model, established by the method, of the bacteria bees and / or the sterile bees. The method is easy and convenient to operate and low in cost, the motion distance and the motion speed of the built model bee are remarkably decreased, obvious dyskinesia is shown, and the survival rate of 5 days in an existing bee Parkinson model building technology is effectively increased to about 80% of 7 days from 5 days lower than 30%. The bee Parkinson's disease model established by the method can be used for screening new methods or new drugs for preventing or treating dyskinesia related diseases.

The invention relates to the technical field of preparation of bioactive peptides, in particular to casein hydrolysate capable of improving bifunctional activity and a preparation method of the casein hydrolysate. According to the research, casein is taken as a raw material, and the casein hydrolysate with high-activity alpha-amylase inhibitory activity and DPPH free radical scavenging rate is prepared through ultrasonic-assisted enzymolysis. Compared with a traditional casein peptide preparation method, the operation technology is simple, the whole technological process can meet the food-grade requirement, and the casein peptide preparation method can be applied to the fields of medicine, health care products, food and the like.

The invention provides hypoallergenic eye-protecting children milk tablets and a preparation method thereof, and the hypoallergenic eye-protecting children milk tablets comprise hypoallergenic formula whey protein powder, casein hydrolysate freeze-dried powder, eye-protecting composite active ingredients, a flavoring agent, an antioxidant and auxiliary materials, wherein the low-sensitization formula whey protein powder comprises a mixture of lactoferrin powder, alpha-lactalbumin powder and low-sensitization beta-LG protein powder, and the low-sensitization beta-LG protein powder comprises a non-covalent compound formed by combining a small molecule substance and cow milk beta-LG; the casein hydrolysate freeze-dried powder comprises bovine milk casein hydrolyzed by Alcalase protease and bacillus licheniformis protease. The selected small molecule substances are all dairy product available additives permitted by GB2760-2024 National Food Safety Standard Food Additives Usage Standards, and related reports of the food additives for beta-LG sensitization improvement do not exist in the existing research at present, so that the blank in the prior art is filled, and the eating safety of children is guaranteed.

The invention belongs to the technical field of polypeptide extraction, and relates to an anti-stress polypeptide composition and a preparation method thereof. A novel functional peptide group with a specific amino acid sequence combination (such as short peptides rich in tyrosine, proline and glutamine) is enriched from complex casein hydrolysate through an innovative separation strategy, the biological activity of the novel functional peptide group is remarkably superior to that of a traditional crude hydrolysate, and the novel functional peptide group belongs to a brand-new functional active substance for feed or veterinary medicine. Therefore, the traditional Chinese medicine composition not only can be used as an auxiliary treatment preparation in veterinary clinic for intervening stress, inflammatory bowel disease or immunosuppression and other diseases, but also can be used as a functional feed additive to be widely applied to daily ration of livestock and poultry and pets, the disease resistance and production performance of animals are improved, the market application prospect is wide, and the traditional Chinese medicine composition has important economic and social values.

The invention provides a preparation method and application of a potassium promoting type microbial fertilizer. The fertilizer is prepared by compounding paenibacillus mucilaginosus functional microspheres, activated bacterial powder and citric acid modified biochar. The inner core of the paenibacillus mucilaginosus functional microsphere is a diatomite-casein hydrolysate porous nutrition carrier loaded with thalli and provides physical support and endogenous nutrition, and the shell of the paenibacillus mucilaginosus functional microsphere is a calcium alginate gel layer and is embedded into a sinapic acid molecularly imprinted polymer microsphere; when root systems of target crops such as rape and leaf mustard secrete sinapic acid due to potassium deficiency, the molecules are specifically recognized and combined to trigger shell degradation, so that internal functional bacteria are released as required, the released bacteria activate soil mineral potassium, the modified charcoal adsorbs and stores potassium ions, and loss is reduced. Matching of microbial inoculum release and crop potassium demand signals is achieved, and the survival rate, the action pertinence and the potassium utilization efficiency of the microbial inoculum are improved.

A casein hydrolysate-chlorogenic acid non-covalent complex emulsion as well as a preparation method and application thereof belong to the technical field of food processing. In view of the fact that the prior art cannot simultaneously consider the alpha-glucosidase inhibitory activity, emulsification stability and antioxidant activity of the casein hydrolysate, the casein hydrolysate-chlorogenic acid complex is prepared through non-covalent interaction, and the complex is used as an emulsifier to construct a functional emulsion. The interaction mainly occurs through hydrogen bonds and hydrophobic interaction, resulting in the unfolding of protein molecular structure and the fluorescence quenching. Meanwhile, the functional emulsion has good emulsification activity and stability. The emulsion prepared by using the complex has the smallest particle size, the lowest turbidity and uniform droplet distribution. The functional emulsion obtained by the application has the antidiabetic effect and excellent emulsification performance, and has important application value in the development of functional foods.

The invention provides a muscle-building, shaping and fat-controlling composition as well as a preparation method, application and a product, and relates to the technical field of foods. The composition is prepared from the following raw materials in parts by weight: 30 to 100 parts of branched chain amino acid, 1 to 2 parts of casein hydrolysate, 10 to 50 parts of collagen peptide powder, 0.4 to 2 parts of cortex cinnamomi powder, 0.5 to 2 parts of purple tea concentrated powder, 5 to 10 parts of black pepper extract, 1 to 2 parts of muskmelon concentrated freeze-dried powder and 2 to 8 parts of d-ribose. Wherein the black pepper is mixed with an ethanol solution, and extraction is carried out by adopting a heating condition assisted microwave treatment mode, so that the black pepper extract is obtained. In the composition disclosed by the invention, all the components interact with one another and have a synergistic effect, so that the obvious effects of gaining muscles, shaping and controlling the fat content are achieved. The composition disclosed by the invention is applied to products with muscle building, shaping and fat control effects, and the effects are remarkable.

The present invention belongs to the technical field of bioactive peptides. Provided are a casein-derived active peptide capable of delaying telomere attrition and the use thereof, and a drug containing the active peptide. An active peptide having the sequence of FVAPFPE is selected from a casein hydrolysate prepared via a fermentation method. When ingested orally, the active peptide can stably cross the blood-brain barrier and exhibits anti-inflammatory, antioxidative and telomere attrition-protective effects. The active peptide can delay aging, and is thus expected to be used for preventing and / or treating aging-related diseases.

The invention relates to the technical field of medicine, in particular to herbal paste for promoting children to grow tall and a preparation method and application thereof. The herbal paste is prepared from the following raw materials in parts by weight: 30-40 parts of Chinese yam, 25-35 parts of lily, 18-25 parts of poria cocos, 25-35 parts of mulberry, 3-8 parts of astragalus membranaceus, 2-5 parts of casein hydrolyzed peptide, 1-3 parts of hydrolyzed yolk powder, 3.5-4.5 parts of yellow pear concentrated juice powder and 0.3-0.8 part of mogroside. The herbal ointment prepared from specific components and specific dosage by the specific preparation method has a better effect of promoting children to grow tall, and the effect is superior to that of each pair of components.

The invention relates to the technical field of food processing, and particularly provides casein hydrolysate capable of improving xanthine oxidase inhibitory activity and a preparation method of the casein hydrolysate. The method comprises the following steps: (1) carrying out ultrasonic pretreatment on casein by adopting different powers; (2) performing enzymolysis on the pretreated casein by using alkaline protease, and performing enzyme deactivation to obtain hydrolysate; and (3) determining the hydrolysis degree, molecular weight distribution, xanthine oxidase inhibitory activity and ROS level of the obtained hydrolysate. Through combination of ultrasonic pretreatment and an enzymolysis technology, experiments prove that the process can significantly improve the xanthine oxidase inhibitory activity of the casein hydrolysate. The process is simple and controllable, the whole process meets the food-grade safety standard, and the prepared casein hydrolysate has important application value in development of anti-hyperuricemia functional food and healthy food.

The invention discloses a casein hydrolysate as well as a preparation method and application thereof. The present invention provides a casein hydrolysate in which the content of a protein component having a molecular weight between 196 Da and 500 Da is 40% by mass or more among the protein components of the casein hydrolysate. The casein hydrolysate provided by the invention has lasting milk fat fragrance.

The application discloses a kind of polypeptide and casein hydrolyzed powder containing the polypeptide and application in promoting lactic acid bacteria proliferation, belong to food microbiological technology field.The polypeptide disclosed in the application has the amino acid sequence of KEGIHAQ.The polypeptide can significantly improve the acid resistance of lactic acid bacteria, and when 0.003-5g / L is added, the viable count of lactic acid bacteria can be increased by 1.2-3 times.The short peptide can be prepared by casein powder hydrolysis, and the casein hydrolysate obtained by hydrolysis and its freeze-dried powder containing the peptide segment also have the effect of promoting lactic acid bacteria proliferation, and are suitable for actual industrial production.

The invention provides freeze-dried brewing grain for assisting long-term administration of chronic diseases of pets as well as a preparation method and application thereof, and relates to the field of pet food. The freeze-dried brewing grain for assisting long-term administration of the chronic diseases of the pets comprises a disease-adaptive freeze-dried staple food grain and administration auxiliary nutrition powder which are mutually independent. The administration auxiliary nutrition powder is prepared from the following components: a yeast extract, pork liver hydrolyzed powder, silicon dioxide micro powder, xanthan gum, guar gum, alpha-casein hydrolysate, L-theanine, a milk thistle extract, S-adenosylmethionine and microencapsulated krill oil. According to the freeze-dried brewing grain for assisting long-term administration of the chronic diseases of the pets, on the premise of seamlessly integrating the pet administration process, the medicine smell is effectively and efficiently masked, long-term administration is facilitated, and the nutritional requirements of the pets suffering from the chronic diseases are further met; therefore, the problem that long-term oral administration of pets is difficult is solved, stress and anxiety of the pets and owners are relieved, and targeted nutrition support is synchronously provided for the pets.

The present invention relates to a method of preparing a casein hydrolysate by hydrolysis of casein with endogenous plasmin, and the casein hydrolysate obtainable by the method. In particular, the present invention relates to a method of preparing a casein hydrolysate without adding any exogenous enzymes such that an organic casein hydrolysate is obtained.

The invention discloses a special culture medium for tissue culture and rapid propagation of caulis spatholobi and an efficient propagation method, and relates to the technical field of plant biology. The special culture medium contains a callus induction and proliferation culture medium, an adventitious bud differentiation culture medium, an adventitious bud proliferation culture medium and a rooting culture medium which are respectively based on MS or 1 / 2 MS, the hormone ratio is optimized, special additives such as casein hydrolysate and inositol are added, and a differentiation-promoting and anti-browning synergistic microenvironment is constructed. According to the breeding method, fine parameters of explant selection, disinfection, culture in each stage and acclimatization and transplantation are defined, the bottleneck that the caulis spatholobi callus is difficult to differentiate into buds is effectively broken through, the browning rate, the multiplication coefficient and the transplantation survival rate are reduced, the technical repeatability is good, the stability is high, and reliable technical support is provided for industrialized production of caulis spatholobi seedlings.

This agent for enhanced pumping up or for enhanced motivation contains a casein hydrolysate as an active ingredient, the hydrolysate containing a tripeptide of at least one of Ile-Pro-Pro and Val-Pro-Pro. The agent for enhanced pumping up contains at least one tripeptide of Ile-Pro-Pro or Val-Pro-Pro as an active ingredient.