67 results about "Casein hydrolysate" patented technology

The invention discloses ACE (Angiotensin Converting Enzyme) inhibitory octapeptide YI8 derived from bovine cheese protein hydrolysate and application, and relates to the technical field of bioactive peptides, and the technical scheme is characterized in that the ACE inhibitory octapeptide YI8 comprises an amino acid sequence of YKVPQLEI. The ACE inhibitory octapeptide YI8 derived from the cattle cheese protein hydrolysate is obtained by screening from the cattle cheese protein hydrolysate, and is an octapeptide YI8 with ACE inhibitory activity, the in-vitro ACE inhibition rate of the ACE inhibitory octapeptide YI8 derived from the cattle cheese protein hydrolysate is tested to be 70.86% under the condition of the concentration of 1mg / ml, and the ACE inhibitory octapeptide YI8 is suitable for functional food for assisting in lowering blood pressure.

The invention belongs to the technical field of health-care products, and particularly relates to a formula helpful for improving sleep and a preparation method thereof. Comprising the following raw material components in parts by weight: 90-110 parts of spina date seed powder, 45-55 parts of casein hydrolysate peptide powder, 15-22 parts of gamma-aminobutyric acid, 18-22 parts of lucid ganoderma powder, 15-22 parts of lily powder, 18-20 parts of poria cocos powder, 12-15 parts of tea leaf theanine and 1-4 parts of lactobacillus plantarum JYLP-326. According to the formula for improving sleep provided by the invention, the limitation of an existing insomnia treatment means is broken through through a three-dimensional synergistic network of traditional Chinese medicines, probiotics and functional components, and the formula realizes systematic improvement on insomnia core symptoms through multi-channel synergy of nerve regulation, intestinal brain axis intervention and immune balance, namely rapid induction of sleep.

The invention discloses an application of enzymatic casein in promotion of porcine endocrine cell proliferation, belongs to the technical field of animal nutrition and feed, and provides an application of enzymatic casein in promotion of porcine endocrine cell proliferation, which does not aim at diagnosis and treatment of diseases. The invention provides a method for promoting pig endocrine cell proliferation by using casein enzymolysis. The method comprises the following steps: adaptively feeding pigs with daily ration with the crude protein content of 13% for 7 days; mixing the daily ration with the crude protein content of 13% with the enzymolysis casein according to the mass ratio of 95.5: 4.5 to obtain the daily ration containing the enzymolysis casein, and feeding twice a day for 28 days. According to the invention, a certain proportion of casein hydrolysate is added into the feed to regulate and control microbe composition of small intestinal mucosa of growing pigs, so that proliferation of small intestinal endocrine cells is further regulated, the secretion level of gastrointestinal hormone is increased, the gastrointestinal transport function of animals is finally enhanced, the absorption of nutrient substances is accelerated, and the growth performance of the pigs is improved.

The invention discloses an improved self-induction culture medium and a culture process, and belongs to the technical field of bioengineering. The improved self-induction culture medium is prepared from 10 g / L of glucose, 3-5 g / L of glycerin, 3 g / L of lactose, 24 g / L of yeast extract, 8 g / L of casein hydrolysate, 0.02-0.05 mM of malonic acid, 1 mM of methionine and 1-3 mM of MgSO4, a phosphate buffer solution is added to control the pH to be 7.2, and the improved self-induction culture medium is prepared by mixing after sterilization. Cells are inoculated to an improved self-induction culture medium for culture, automatic switching of growth-induction stages can be realized based on a pH and DO linkage dynamic feeding control method, the operation complexity is reduced, and the controllability is higher. The culture medium does not need a special inducer, cost is reduced, and protein expression quantity is improved.

The invention discloses a preparation method and application of hydrolyzed casein peptide capable of reducing uric acid and facilitating gout attack prevention and gout symptom improvement, belongs to the technical field of biology, and has a wide application prospect. The casein hydrolysate peptide prepared by the method disclosed by the invention can effectively reduce uric acid, and is beneficial to preventing gout attack and improving gout symptoms by the following ways: 1, inhibiting the activity of xanthine oxidase (UA enzyme), and reducing the generation of uric acid; 2, metal ions such as Ca < 2 + > and Na < + > are complexed to prevent generation of urate crystals, so that gout attack can be prevented; 3, the microenvironment of an articular cavity is improved, urate crystals are dissolved, and the gout symptom can be improved; and 4, liver and kidney inflammation caused by high uric acid is relieved.

The present invention belongs to the technical field of functional oligopeptides, and discloses a hydrolyzed casein oligopeptide with auxiliary blood pressure lowering function and its preparation method and application. The present invention uses casein as raw material, collects casein hydrolyzate containing active peptides through directional and controllable enzymolysis, and obtains the above-mentioned hydrolyzed casein oligopeptide with significant blood pressure lowering function. It also has the effect of lowering blood lipids, improving endothelial function and vascular elasticity. In the present invention, the main sequence of the hydrolyzed casein oligopeptide with blood pressure lowering function containing the hydrolyzed casein oligopeptide with blood pressure lowering function is VNEISK, LQSW, VLPVPQK, MIMKEP, GPFPIIV, FFVAPFPEVFGK, wherein VNEISK is the hydrolyzed casein oligopeptide with the auxiliary blood pressure lowering function newly discovered in this study with the largest activity and the highest content, which has the effect of assisting in lowering blood pressure, softening blood vessels, improving vascular elasticity, and improving headaches, dizziness, palpitations, tinnitus, insomnia and the like caused by hypertension. The blood pressure lowering peptide prepared by the present invention can be used in various forms of products such as milk powder, beverages, and tablet candies.

The present invention provides a method for preventing or treating inflammatory skin conditions, by administering a composition comprising at least one milk derived protein. The present invention further relates to a method for moisturizing dry skin, skin softening, reducing skin itching, reducing skin peeling, reducing skin redness, reducing rough skin, reducing skin swelling, reducing skin cracking, renewal skin cells, preserving hand skin or any combination thereof.

The invention discloses a cyclamen tissue culture propagation method taking ovules as explants, and belongs to the technical field of plant tissue culture. The method comprises the steps of ovule inoculation, callus induction and screening, bud induction and proliferation, strong seedling culture, rooting induction and acclimatization and transplantation. According to the method, casein hydrolysate nutrition supporting, dynamic hormone regulation and control, bud cluster-shaped proliferation, strong seedling rooting, combination of in-bottle seedling hardening and out-bottle seedling hardening, transplanting matrix improvement and other methods are adopted, and an effective new way is provided for improving the cyclamen tissue culture inoculation survival rate and the seedling emergence survival rate.

The invention discloses a novel ginseng stem cell culture system which comprises a basic culture medium and a growth regulation composition, and the basic culture medium comprises ammonium sulfate, monopotassium phosphate, magnesium sulfate heptahydrate and calcium chloride; the growth regulating composition comprises 2, 4-dichlorphenoxyacetic acid, zeatin, salicylic acid and natural polysaccharide. The basic culture medium also comprises casein hydrolysate, coconut water extract and cane sugar. The composition also comprises an anti-oxidation composition. The invention further discloses a disposable flexible bioreactor for ginseng stem cell culture, the bioreactor comprises a tank body made of a flexible material, an air distribution device, a sterile sampling device and an online parameter monitoring device, and the top of the tank body structure is a cylindrical body and is provided with an inoculation opening, a culture medium liquid supplementing opening, an air outlet and a sampling opening; the bottom is an inverted cone provided with a parameter monitoring device interface and an air inlet, and the air inlet is connected with a ventilation device and an air flow adjusting device. The invention also discloses application of the culture system and the bioreactor.

The invention relates to a casein hydrolysate for the treatment of Crohn's disease. The composition of the casein hydrolysate is determined by the use of a proline- specific endoprotease for the hydrolysis of casein and is characterized in that the molar fraction of peptides of said hydrolysate carrying a carboxy terminal proline, expressed in %, is more than two times the molar fraction of proline, expressed in %, in the casein substrate used to generate the hydrolysate.

The present invention relates to a culture medium composition for producing botulinum toxin, and more particularly, to a culture medium composition for culturing Clostridium species capable of producing botulinum toxin. The culture medium composition of the present invention comprises casein hydrolysate and at least one plant-derived proteose peptone selected from the group consisting of pea hydrolysate, cottonseed hydrolysate, and wheat gluten hydrolysate. When Clostridium botulinum is cultured using the culture medium according to the present invention containing plant-derived proteose peptone, casein hydrolysate, and minerals, the growth rate of the bacteria in the culture medium is higher than that in each of the currently used culture medium and the culture medium containing only plant-derived proteose peptone. In addition, when the culture medium of the present invention is used, it is possible to produce a high concentration of botulinum toxin by culturing the bacteria in a safe manner.

The invention relates to a composition with an effect of improving sleep quality during pregnancy and application of the composition. The composition comprises aminobutyric acid, tea leaf theanine, casein hydrolysate and spearmint extract. The aminobutyric acid, the tea theanine, the casein hydrolysate and the spearmint extract are compounded for use, so that the problem that a single-mechanism sleep-aiding product has a limited effect on insomnia during pregnancy is solved, the sleep structure is improved to a great extent, and the sleep quality is improved.

The invention discloses a casein hydrolysis protease fermentation process, which comprises strain breeding and preparation, fermentation medium preparation, fermentation condition control and fermentation process monitoring and adjustment, a layered nutrition supply system comprising multiple layers of edible biological membranes is adopted in the fermentation process, and each layer in the system contains specific trace elements. Different microelements are released layer by layer by regulating and controlling the decomposition time and sequence of each layer of biological membrane in the fermentation process, so that the requirements of thalli on each stage in the casein protease hydrolysis fermentation process are met, and the antagonism among the microelements is reduced; according to the multi-layer biological membrane dynamic nutrition supply system disclosed by the invention, by accurately releasing nutrient elements, key defects in the prior art are overcome, and the efficiency, stability and economical efficiency of a casein hydrolysate protease fermentation process are remarkably improved; the process has wide industrial application potential, and the quality and market value of the protease product can be remarkably improved.

The invention relates to a fermentation medium of porcine circovirus type 3 (PCV3) Cap protein virus-like particles and a fermentation method thereof. The fermentation culture medium comprises tryptone, a yeast extract, casein hydrolysate, sorbitol, glycerol, polyoxyethylene mono-tert-octyl phenyl ether and the like. Methanol is supplemented for three times in the fermentation process, so that the expression efficiency of the Cap protein of PCV3 is remarkably improved, and correct folding and self-assembly of the Cap protein into the virus-like particles are promoted. According to the culture medium and the method, generation of impure protein is reduced, the purification cost is reduced, and key technical support is provided for industrial production of PCV3 subunit vaccines.

The application provides a muscle building and shaping, fat control composition, a preparation method, application and product, and relates to the field of food technology.The composition according to the application is composed of the following raw materials in parts by weight: 30-100 parts of branched chain amino acid, 1-2 parts of casein hydrolysate, 10-50 parts of collagen peptide powder, 0.4-2 parts of cinnamon powder, 0.5-2 parts of purple tea concentrated powder, 5-10 parts of black pepper extract, 1-2 parts of concentrated freeze-dried watermelon powder and 2-8 parts of d-ribose; wherein the black pepper and ethanol solution are mixed, and the black pepper extract is obtained by means of extraction under the condition of heating and auxiliary microwave treatment. In the composition according to the application, the components interact with each other, and the muscle building, shaping and fat control effects are synergistically improved. The composition according to the application is applied to products with the effects of muscle building, shaping and fat control, and the effects are remarkable.

This invention relates to the field of food processing technology and discloses a process for preparing plant-based beverages containing casein hydrolysate peptides. The process includes: preheating a base liquid containing casein hydrolysate peptides and anionic polysaccharides and pumping it into a Venturi channel; inducing polysaccharide volume expansion by utilizing the hydrostatic pressure drop in the venturi channel to construct an isolation layer; simultaneously aspirating a low-temperature plant polyphenol extract, using the temperature difference to form an adhesion interface on the surface of the isolation layer; after the mixed fluid enters the expansion section, using the compressive force generated by hydrostatic recovery to press the polyphenols into the polysaccharide gaps, constructing a composite physical isolation phase; and triggering polysaccharide thermal gelation crosslinking by heating to establish a physical barrier. This invention utilizes the microscopic encapsulation mechanism generated by the fluid pressure field and temperature gradient to effectively block contact between polyphenols and protein molecules, inhibit complexation precipitation under thermal shock, and ensure that the beverage system possesses excellent thermal stability and storage stability.

The application provides a lacto-casein hydrolysate with anti-inflammatory effect and a preparation method and application thereof.The lacto-casein hydrolysate of the application comprises a peptide segment FAWPQYLK, a peptide segment LPW, a peptide segment MPLW and a peptide segment PLW, can reduce the release of pro-inflammatory cytokines, such as IL-1beta, IL-2, IL-6 and TNF-alpha, and improve the release of anti-inflammatory cytokines, such as IL-10, so as to reduce the cell inflammatory response, and further achieve the anti-inflammatory effect.

The invention relates to sleeping milk and a preparation method thereof. The sleeping milk is prepared from the following raw materials: raw milk, theanine, casein hydrolysate, alpha-lactalbumin, sucrose fatty acid ester, glyceryl monostearate, glyceryl distearate, buffer salt and a compound extract, the compound extract comprises a combination of an oat kernel extract, a lotus seed extract, a red date extract and a banana extract. According to the invention, the theanine, the casein hydrolysate, the alpha-lactalbumin and the composite extract are combined for use, and the oat kernel extract, the lotus seed extract, the red date extract and the banana extract are added, so that the four components can interact with one another and have mutual normal-phase influence; the product has an excellent synergistic promotion effect in the aspects of soothing the nerves and helping sleep, and can further improve the sleep-soothing effect of the product after being added into the dairy product.

The invention provides a composition for promoting protein peptide absorption by utilizing molecular association and application of the composition to deep sleep promotion. The composition is prepared from a passion flower extract, casein hydrolysate, gamma-aminobutyric acid, collagen tripeptide, associated molecules and vitamin B complex. According to the composition, absorption of protein peptides is promoted through molecular association, gastrointestinal tract absorption disorders are reduced, all the components in the composition supplement one another and interact with one another, and the effect of promoting deep sleep can be effectively achieved. The oral preparation described in the invention can be a drug loading system such as a capsule, a tablet, a granule and the like.

The honey freeze-dried powder comprises the following main components in parts by weight: 10-15 parts of honey, 0.1-2 parts of a foaming agent, 5-15 parts of a filling agent, 10-15 parts of water and 0-0.15 part of an anti-caking agent. The invention further discloses a preparation method of the honey freeze-dried powder. The casein hydrolysate is used as a foaming agent, and honey, a filling agent and an anti-caking agent are added, so that the honey freeze-dried powder which is clean in ingredient, simple in process, loose in structure and easy to dissolve in water, and retains the nutrition and flavor of honey is prepared.

The present invention discloses a tissue culture and propagation method of Cyclamen using ovules as explants, belonging to the technical field of plant tissue culture. The method includes steps of ovule inoculation, callus induction and screening, bud induction and proliferation, strong seedling culture, rooting induction, and acclimatization and transplantation. By adopting methods such as using casein hydrolysate for nutritional support, dynamic hormone regulation, bud cluster proliferation, strong seedling state rooting, combination of in-vial acclimatization and ex-vial acclimatization, and improvement of transplantation substrate, the present invention provides an effective new way to improve the survival rate of Cyclamen tissue culture inoculation and seedling emergence.

The invention relates to a construction method of an Agrobacterium rhizogenes mediated acacia melanoxylon genetic transformation system, which comprises the following steps: by taking an acacia melanoxylon SR17 sterile single plant as an explant, selecting an Agrobacterium rhizogenes strain Ar1193, infecting for 45 minutes in a heavy suspension of which the bacterial liquid concentration OD600 is equal to 0.9 and the acetosyringone concentration is 100 [mu] mol.L <-1 >, co-culturing for 3 days, and then transferring into a bacteriostatic culture medium to induce hairy roots; and taking the transgenic hairy roots obtained by induction as explants, and inoculating the explants into a WPM culture medium containing 0.05 mg.L <-1 > TDZ and 200 mg.L <-1 > casein hydrolysate to induce regeneration of adventitious buds so as to obtain complete transgenic plants. The screening concentrations of the four antibiotics, namely kanamycin, hygromycin, glyphosate and glufosinate-ammonium, are determined; a stable and efficient acacia melanoxylon genetic transformation system is established, the hairy root induction rate reaches 63.17%, the transgenic positive rate reaches 15.42%, and the adventitious bud regeneration rate reaches 52%.

The present invention discloses a fermentation medium for high-yield dextran production from Leuconostoc mesenteroides GFMO1. The medium for the growth of the bacteria is formulated from the sucrose agar medium, whereby the yield of the Exopolysaccharide produced is increased upon the addition of casein hydrolysate and bacteriological peptone in the fermentation medium. Casein acidic hydrolysate and bacteriological peptone are defined sources of amino acids that supplement organic growth factors such as vitamins. The main role of the defined source is to reduce the fermentation time of the bacteria, enhance viable numbers of lactic acid bacteria, and promote the production yield..

The invention discloses a litsea cubeba secondary embryo proliferation culture medium and a proliferation culture method, the culture medium is based on an MS culture medium, cane sugar, agar, casein hydrolysate, 6-benayl aminopurine, naphthylacetic acid, inositol and cyclodextrin are added, and the pH value is 5.6-5.8; the method comprises the following steps: a somatic embryo generation stage, a secondary embryo proliferation stage and regular removal of mature somatic embryos. According to the method, the immature seeds are adopted as explants, through the somatic embryogenesis culture medium and the secondary embryo proliferation culture medium of a specific formula, dark culture and periodic subculture are combined, the proliferation efficiency of the secondary embryos is remarkably improved, the highest proliferation multiple can reach 20.17 times, meanwhile, the endophyte pollution risk is reduced, and the genetic stability is guaranteed. The method is simple and convenient to operate, short in culture period, low in cost and suitable for large-scale industrial application, provides important technical support for rapid propagation, germplasm preservation and industrial development of litsea cubeba, and has remarkable economic and social benefits.

The invention provides a modified milk-derived active peptide loaded with probiotics and application of the modified milk-derived active peptide in a sleep-aiding milk beverage, polyethylene glycol is introduced into casein hydrolysate decapeptide YLGYLEQLLR for modification to prepare modified polypeptide, hydrogel is formed based on self-assembly of the modified polypeptide, and the probiotics are embedded to prepare the modified milk-derived active peptide loaded with the probiotics. The modified milk-derived active peptide can embed and slowly release the probiotics, so that the probiotics are continuously released in vivo, and the sleep quality is improved; in addition, the probiotics and the polypeptide synergistically play a role, so that the mood is regulated, and the sleep quality is improved.

The invention relates to the technical field of nutrition and health, in particular to a sleep-promoting composition containing oriental cherry polyphenol and a preparation method of the sleep-promoting composition. The oriental cherry polyphenol-containing sleep promoting composition is prepared from the following components in parts by mass: 5 to 8 parts of lemon myrtle leaf powder, 1 to 5 parts of oriental cherry polyphenol, 15 to 20 parts of casein hydrolyzed peptide, 15 to 25 parts of semen ziziphi spinosae powder, 5 to 8 parts of rhizoma gastrodiae powder, 8 to 12 parts of poria cocos powder, 2 to 10 parts of Borojo powder, 1 to 5 parts of N-acetylneuraminic acid, 10 to 15 parts of corn leaf extract and 0.5 to 5 parts of silicon dioxide. The lemon myrtle leaf powder, the sakura polyphenol, the casein hydrolyzed peptide and the N-acetylneuraminic acid are used as main effective components, and the obtained sakura polyphenol composition has the effects of improving sleep, resisting oxidation and improving the immunity of a human body and can be used as health-care food.

The present application provides a nutrient composition including: stachydrine, lactobacillus plantarum, walnut peptide, desert cistanche, casein hydrolysate, and two or more of theanine. The present inventors found that the above nutrient composition has a brain health function in the direction of anxiolytic, antioxidant, neuroprotective, and memory improvement.

The invention discloses a cardiac troponin T calibrator diluent composition, a diluent, a calibrator as well as a preparation method and application of the calibrator. The composition comprises a basic diluent component, a potassium salt, a casein hydrolysate and dodecyl trimethyl ammonium bromide, and the mass ratio of the potassium salt to the casein hydrolysate to the dodecyl trimethyl ammonium bromide is (5-39): (1-20): (0.5-3). According to the provided cardiac troponin T calibrator diluent composition and diluent, by optimizing the variety and concentration in the formula, the stability of cardiac troponin T in a liquid state and a freeze-drying state is remarkably improved, adsorption and degradation of cardiac troponin T in a glass container are effectively inhibited, the cardiac troponin T can be stabilized in a room-temperature glass bottle, and the application range of the cardiac troponin T calibrator diluent composition is widened. And after redissolution, good concentration consistency is still maintained, and reliable stability guarantee is provided for preparation, freeze-drying and clinical detection of the cardiac troponin T calibrator.

The present invention belongs to the technical field of plant propagation and cultivation, and in particular relates to a basal culture medium, a tissue culture medium, and a regeneration tissue culture method for regenerating long-barreled Lycoris radiata. The present invention provides a tissue culture medium for regenerating long-barreled Lycoris radiata, the tissue culture medium comprising an induction medium, a proliferation medium, a differentiation medium, a bud-strengthening medium, and a seedling-strengthening and rooting medium; in the tissue culture medium provided by the present invention, a modified MS medium and a modified B5 medium are used as the basal culture medium, and the combined action of 2,4-D, 6-BA, IBA, NAA, carbon nanotubes, and casein hydrolyzate facilitates the induction of long-barreled Lycoris radiata callus formation, proliferation, differentiation, and rooting, and sucrose, an energy substance, is combined to jointly improve the propagation coefficient of the long-barreled Lycoris radiata, thereby obtaining a large number of long-barreled Lycoris radiata tissue culture seedlings.

The present invention provides a method of preventing or treating inflammatory skin diseases by administering a composition comprising at least one milk-derived protein. The invention also relates to a method for moisturizing dry skin, softening skin, reducing skin itch, reducing skin desquamation, reducing skin redness, reducing rough skin, reducing skin swelling, reducing skin dehiscence, renewing skin cells, protecting hand skin or any combination thereof.