203 results about "Casein hydrolysate" patented technology

The invention provides a novel fourth-generation infant formula. The novel fourth-generation infant formula comprises the following ingredients: lactose, desalted whey powder, 1,3-dioleoyl 2-palmitoyl triglyceride, hydrolyzed whey protein, fructo-oligosaccharide, galactooligosaccharide, mineral substance premix compound, casein hydrolysate, whey protein concentrate, soyabean lecithin, vitamin premix, lactoferrin, L-tyrosine, nucleotide, L-tryptophan, L-methionine and the like. The infant formula is added with appropriate amount of 1,3-dioleoyl 2-palmitoyl triglyceride, appropriate amount of protein hydrolysate, monomer amino acid and the like, mineral contents such as phosphorus, sodium and the like in milk powder are reduced, therefore, the fat structure, the amino acid composition, the amino acid content and the osmotic pressure in the milk powder are close to those of the breast milk, the infant formula is balanced and comprehensive in nutrition and is appropriate in composition, all substances have synergistic effect, the bioavailability of protein and the fat is effectively improved, and the infant formula can promote digestive absorption and skeletal development of babies and can reduce the visceral organ burden.

The present invention relates to amino acid humanized infant formula milk powder and a preparation method thereof. The amino acid humanized infant formula milk powder comprises the following materials by a certain weight percent: lactose, demineralised whey powder, vegetable oil, skimmed milk powder, hydrolyzed whey protein, galactooligosaccharides, fructo oligosaccharide, a mineral premix, ARA, casein hydrolysate, whey protein concentrate, soybean phospholipids, DHA, a water-soluble vitamin premix, casein phosphopeptides, lactoferrin, L-tyrosine, nucleotide, a lipid soluble vitamin premix, L-tryptophan, L-methionine and taurine. The present invention also includes a method for preparing the amino acid humanized infant formula milk powder. The present invention can effectively solve the problem of pool protein absorption and digestion effect and the low protein utilization rate of infants during infant period.

The invention provides a heatproof freeze-drying protective agent, live vaccine freeze-drying powder and a preparation method of the live vaccine freeze-drying powder, and relates to the field of microorganism deposit. The heatproof freeze-drying protective agent comprises 46-90% of oligosaccharide, 1-9% of amino acid, 1-9% of gelatin, 1-9% of casein hydrolysate, 1-9% of polyvinyl pyrrolidone, 1-9% of glycerol and 1-9% of pluronic 68. The preparation method of the live vaccine freeze-drying powder comprises the steps of preparing a protective agent solution and the live vaccine, drying, keeping for 2-6h at -10-2 DEG C and atmospheric pressure, keeping 10-26h for 0-20 DEG C at 100-800mbar, and keeping 2-8h at 0.01-50.mbar and 10-28 DEG C. The heatproof freeze-drying protective agent is reasonable in component and content collocation, and allows the live vaccine to be heatproof. According to the preparation method of the live vaccine freeze-drying powder, formation of ice crystals is avoided, and the obtained live vaccine freeze-drying powder can be kept for 3 months at 37 DEG C and 18 months at 20 DEG C.

The invention provides a method for building an efficient regeneration system of Yunnan rhododendrons and belongs to the field of a plant biotechnology. The method uses upper-middle portion leaves of Yunnan rhododendron aseptic seedlings as a material, one or more of hormones TDZ, ZT, 2,4-D, NAA, IAA and casein hydrolysate with appropriate concentration are mainly provided based on a woody plant medium (WPM) culture medium, and complete plants are obtained through a direct leaf regenerating way and an indirect leaf regenerating way. The method builds the efficient regeneration system of the Yunnan rhododendrons for the first time, provides a good theoretical basis and an experimental basis for genetic engineering breeding work of the Yunnan rhododendrons and simultaneously promotes biotechnology breeding progress of the rhododendrons effectively.

The invention relates to formula nutrition food capable of effectively relieving stress and improving sleep and a preparation method. The formula nutrition food is prepared from the following components in percentage by weight: 4.5 to 62.5.0 percent of gamma-aminobutyric acid, 4.0 to 50.0 percent of tea theanine, 3.0 to 35 percent of a casein hydrolysate, 10 to 25 percent of an asparagus powder, 8to 25 percent of a spina date seed powder, 1.53 to 5.90 percent of L-calcium lactate, 0.0007 to 0.0022 percent of vitamin B6 and 10 to 25 percent of a sweetening agent. The dosage form of the formulanutrition food is tablet sugars, solid drink and drink. The formula nutrition food disclosed by the invention is natural, does not have toxic and / or side effects, and can effectively relieve the stress and improve the sleep quality.

The invention discloses a preparation method of a natural coating antistaling agent for fresh meat preservation. The preparation method comprises the following steps of: A1, preparation of antistaling components in edible fungi; A11, preparation of aromatic components of the edible fungi; A12, preparation of polysaccharide and polypeptide of the edible fungi; A2, preparation of casein hydrolysate; and A3, preparation of the antistaling agent. The coating antistaling agent comprises the following components in parts by weight: 10-15 parts of an antistaling material A, 30-40 parts of an antistaling material B, 10-20 parts of an antistaling material C, 5-10 parts of Nisin, 5-10 parts of citric acid, 5-10 parts of sodium erythorbate, and the balance of sterile water. The components are evenly mixed to obtain 100 parts of the coating antistaling agent for later use. The preparation method scientifically combines edible fungi protein, edible fungi aromatic substances, edible fungi polysaccharide and casein hydrolysate (rich in antibacterial peptide and antioxidant peptide) to obtain the natural fresh meat coating antistaling agent, and the shelf life of fresh meat is prolonged while the nutrient composition of the fresh meat is enriched.

The invention provides a heat-resisting protective agent and an application thereof, and relates to the field of live microorganism deposit. The heat-resisting protective agent comprises the following substances: 40-80% of oligosaccharide, 3-12% of amino acid, 3-12% of gelatin, 3-12% of casein hydrolysate, 3-12% of polyvinyl pyrrolidone, 3-12% of glycerol, and 3-12% of poloxamer-68. The invention further provides the application of the heat-resisting protective agent in preparation of live vaccine freeze-dried powder. The heat-resisting protective agent is dissolved with a solvent; a protective agent solution is obtained; the protective agent solution is mixed with live virus liquid; a live vaccine is obtained; the live vaccine is freeze-dried; and then the live vaccine freeze-dried powder is obtained. The heat-resisting protective agent can improve heat resistance of the live vaccine, and the live vaccine freeze-dried powder prepared by the heat-resisting protective agent is stored for 2 months at 37 DEG C, and stored for 24 months at 15 DEG C.

The invention discloses a rapid proliferation method for anoectochilus formosanus tissue culture, which comprises the steps: cleaning and disinfecting an explant, inoculating the explant after cleaning and disinfecting in an MS culture medium, performing low light culture for one week, then performing strong light culture for one week, wherein the illumination time is 10 hours per day, and the temperature is 23+ / -2 DEG C; culturing for 40 days; inoculating the explants after culturing in a liquid culture medium of the MS culture medium, 1.4-1.6mg / L of 6-BA and 0.2-0.3mg / L of NAA in a ratio for enrichment culture under a dark culture condition, wherein the temperature is 23+ / -2 DEG C, and the culture period is 45 days; and by using a strong seedling culture medium as the MS culture medium, performing illumination culture, wherein the illumination time is 8 hours per day, the temperature is 23+ / -2 DEG C, the culture period is 12-15 days, and the ratio of a rooting culture medium is as follows: the content of MS is 1 / 2, the content of bananas is 60g / L, the content of active carbon is 1g / L, the content of casein hydrolysate is 0.5g / L, the illumination time is 10 hours per day, the temperature is 23+ / -2 DEG C, and the culture period is 3 months. According to the rapid proliferation method for the anoectochilus formosanus tissue culture, the industrialized rapid proliferation is realized, and the culture period is shortened.

The invention discloses a method for breeding locusts, which uses zygotic embryo of different embryonic ages as explants, and uses MS+2, 4-D+BA+cane sugar+agar as an induction medium of an embryonic callus. The method comprises: using MS basal medium + MES + glutamine + casein hydrolysate + naphthylacetic acid + 6-Benzylaminopurine+cane sugar+agar as a somatic cell embryo medium, by inducting thecallus, transferring the formed globular embryo onto MS+casein hydrolysate medium, carrying out maturation culture of somatic embryo, and bourgeoning the cultured somatic embryo on a basal medium to from a complete small plant. The method of the invention has the advantages of high callus inductivity, high somatic embryo inductivity and high germination rate, and can cultivate a large numbers of locust test-tube plantlets in a short period for large scale and factory production.

The invention discloses a culture medium for in-vitro induced regeneration plants of double-haploid stems of potatoes, which belongs to the field of agricultural biotechnologies. The culture medium comprises the following compositions: potassium nitrate, ammonium nitrate, monopotassium phosphate, magnesium sulfate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, sodium molybdate, copper sulfate, cobalt chloride, ferrous sulfate, disodium ethylenediamine tetraacetic acid, nicotinic acid, pyridoxine hydrochloride, glycine, glutamine, casein hydrolysate, yeast extracts, sucrose, 6-benzylaminopurine, naphthalene acetic acid, kinetin, zeatin and heteroauxin beilining. The culture medium is applied to the induction and culturing of double-haploid stem regenerated seedlings of potatoes, the test effect is good, the initiating rate is increased by 76%, and the seedling rate is as high as 80.5%. The regeneration rate of double-haploid stems of potatoes is significantly increased, thereby laying a foundation for the establishment of a genetic transformation system; and the culturing mode adopts a one-step seedling method, so that the pollution probability is reduced.

The invention discloses an exercise-fatigue-resistant nutrition protein bar. The exercise-fatigue-resistant nutrition protein bar comprises, by weight, 10-100 parts of casein hydrolysate, 20-200 parts of wheat protein, 5-40 parts of HMB-Ca, 100-400 parts of protein powder, 150-500 parts of saccharides, 20-100 parts of lipid and 5-15 parts of purified water. The exercise-fatigue-resistant nutrition protein bar has the advantages that bar-shaped convenient food can be manufactured from the exercise-fatigue-resistant nutrition protein bar, so that athletes and exercise people can conveniently eat the convenient food during long-term exercise; as proved by tests, excellent exercise-fatigue-resistant effects can be realized by the exercise-fatigue-resistant nutrition protein bar, the endurance exercise time can be prolonged, excellent exercise states can be kept, and the exercise capacity can be improved.

The invention discloses an ACE inhibitory peptide and a preparation method thereof, and provides a casein hydrolysate which has an anti-hypertension effect and is rich in the ACE inhibitory peptide, the ACE inhibitory peptide with an anti-hypertension effect, and a preparation method of the ACE inhibitory peptide. The casein hydrolysate rich in the ACE inhibitory peptide contains two peptide fragments with ACE inhibitory activities; and the amino acid sequences of the two peptide fragments with ACE inhibitory activities are respectively YS and YQKFPQYLQY. The amino acid sequence of the ACE inhibitory peptide is YS or YQKFPQYLQY. The ACE inhibitory peptide or the casein hydrolysate rich in the ACE inhibitory peptide is sufficiently mixed with milk powder matrixes to prepare anti-hypertension milk powder; or the ACE inhibitory peptide or the casein hydrolysate rich in the ACE inhibitory peptide is mixed with fresh milk to obtain a mixture, and lactic acid bacteria is inoculated in the mixture to obtain yoghourt with an anti-hypertension effect. The dairy product has a remarkable anti-hypertension effect, has no influence on the normal blood pressure, is safe to eat, and is suitable for being ate by the hypertension groups.

The invention provides a method for screening crude delta sleep-inducing peptide extract from a milk source in virtue of a patch clamp technique. The method comprises the following steps: adding water, trypsin and pepsin in cow's milk for enzymatic hydrolysis and carrying out spray drying after enzyme killing so as to obtain powdery casein hydrolysate; separating the casein hydrolysate through isoelectric precipitation; subjecting a purified product obtained after isoelectric precipitation to preparative liquid chromatography via a reversed-phase steel column so as to separate and purify samples again, dividing the samples into a plurality of sections according to different polarities of the samples and carrying out modeling to evaluate the activity of the crude delta sleep-inducing peptide extract; employing the whole cell patch clamp technique, giving an evoked action potential with a size of 30-60 Pa / 3000-4000 ms, delivering a drug with a dosage of 100 to 150 [mu]mol / L, recording an electrophysiological spectrum and screening out good active sleep-inducing fragments via discharging number and frequency. The method is simple to operate and has good separation effect; and the crude extract can shorten time for going to sleep and adjust sleep rhythm and provides better guarantee for health of patients with insomnia.

The invention discloses a culture method of peony embryonic callus as well as a culture medium. The peony embryonic callus is induced through adopting an MS culture medium which contains special components after modification (such as substances of 6-BA, KT cytokinin, TDZ plant growth regulators, PIC herbicide as well as casein hydrolysate); aiming at a peony embryo explant, the induction rate of the peony callus can achieve 100% by adopting the specific culture method provided by the invention, wherein the induction rate of embryonic callus can achieve 86% above.

The present disclosure provides a method for suppressing a proinflammatory response by administering a composition comprising peptides selected from a casein hydrolysate. Such a composition may reduce the levels of proinflammatory cytokines and may be a treatment for inflammatory disease, especially type 1 diabetes. Preferably, the hydrolysate consists of peptides with a molecular weight of more than 500 Da.

The invention relates to a enzymatical hydrolyzed casein and the method for preparing casein phosphopeptide (CPP) and casein non phosphopeptide (CNPP), belonging to dairy further processing and functional health care food field. The invention takes casein as raw material, takes single alkali prolease Alcalase 2.4L for controlling hydrolysis, takes nitrobenzene-sulfonic acid for pH value monitoring, preparing CPP and CNPP from casein hydrolysate with ethanol- calcium separation method. The nitrogen / phosphorus molar ratio in CPP is 20- 25: 1, the productivity is above 35%; the productivity of CNPP reaches 45%; the determined amine aicd squence of two ACE inhibiting active peptide and one antioxidant active peptide in CNPP product is Tyr-Leu,Glu-Asp-Val-Pro and His-Ser. The invention is characterized by good enzymolysis effect, low price, rational process route, high raw material vailability ratio, and wide application of CPP and CNPP in functional food ingredient or health care food.

Disclosed is a method for preparing lactic acid bacteria having a high anti-allergic activity comprising culturing the lactic acid bacteria in a medium containing casein hydrolysate. The invention also provides an anti-allergic agent and a food / beverage product having anti-allergic activity comprising the lactic acid bacteria cultured in the method of the invention.

The invention relates to the field of health food, in particular to a composition for improving sleep quality and a preparation of the composition. The composition comprises gamma-aminobutric acid and casein hydrolysate. The gamma-aminobutric acid and the casein hydrolysate are composed to use, pentobarbital sodium induced sleeping time can be obviously prolonged, pentobarbital sodium induced sleeping time incubation period is shortened, a function of sleeping improvement is obviously achieved, and good or even better effect can be achieved in case of reducing doses.

The invention discloses hypoallergenic newborn formula milk powder. The hypoallergenic newborn formula milk powder comprises the raw materials of raw milk, lactose, alpha-whey protein, whey protein hydrolysates, casein hydrolysate and bioactive substances. The invention further discloses a method for preparing the milk powder. The method comprises the following steps of (1) uniformly mixing the raw materials with water, and performing complete dissolving so as to obtain feed liquid A, wherein the raw materials comprise the raw milk, the lactose, the alpha-whey protein, the whey protein hydrolysates and the casein hydrolysate; (2) homogenizing the feed liquid A obtained in step (1), sterilizing the homogenized feed liquid A, concentrating the sterilized feed liquid A and performing spray drying so as to obtain a material B; and (3) uniformly mixing bioactive substances in the raw materials with the material B in a dry-mixing workshop so as to obtain the hypoallergenic newborn formula milk powder. The hypoallergenic newborn formula milk powder disclosed by the invention has the advantages of taking account of being hypoallergenic and improving immunity, so that the problem of causing allergic reaction of babies is solved through reducing anaphylactogen and improving immunity. The preparation technology is simple, the milk powder is good in solubility, the shelf life is stable, and the milk powder is not liable to oxidize.

The invention discloses a lactobacillus bulgaricus enrichment culture medium and a preparation method thereof. The lactobacillus bulgaricus enrichment culture medium comprises 0.4 g of lactose, 0.4 mL of new-born calf serum, 0.8 g of casein hydrolysate, 0.090 mL of hydroxyproline with mass concentration of 1 percent, 0.050 mL of glutamic acid with mass concentration of 1 percent and 0.59 g of K2HPO4 in every 100 ml of water, wherein the pH (Potential of Hydrogen) value is 6.5; and the casein hydrolysate is purchased from Rosenbo Science and Technology Limited. The culture medium is a cheap culture medium capable of realizing large-scale growth of lactobacillus bulgaricus. The culture medium has the advantages of simple and cheap ingredients which are all more common carbon and nitrogen sources and easiness in preparation, so that the method is a good method for industrial production of lactic acid bacteria.

The invention provides a nutritional composition capable of promoting wound healing, bedsore restoration, and postoperative stress ulcer healing. The nutritional composition is capable of shortening the length of stay of patients at the perioperative period, reducing medical cost, and reducing mortality. The nutritional composition is prepared via combination of collagen peptide, wheat peptide, and casein hydrolysate at a certain ratio.

The invention discloses microdiet for fish larvae of takifugu rubripes. The microdiet comprises components as follows: white fish meal, tenebrio molitor meal, earthworm powder, shrimp oil, rice protein, husked sorghum flour, lotus root starch, maize germ oil, walnut oil, casein hydrolysate, green-lipped mussel extract, cylindrotheca powder, haematococcus pluvialis powder, a functional additive, complex vitamins and compound minerals. The preparation method of the microdiet comprises steps as follows: 1), preparation of raw materials; 2), synthesis of biological materials, 3), conversion into cooked materials; 4), puffing for granulation; 5), drying; 6), spraying for film coating; 7), vacuum drying. The microdiet can improve the body color of the fish larvae of the takifugu rubripes, promote growth and reduce tetrodotoxin enrichment.

The invention provides a dairy product and a preparation method thereof. Based on the total weight of the dairy product, the dairy product comprises the following raw materials in parts by weight: 30-90 parts of milk, 5-10 parts of whey protein powder, 3-6 parts of sugar, 0.1-0.5 parts of a casein hydrolysate, 0.05-0.5 parts of taurine, 0.05-0.5 parts of lactase, 0.05-0.3 parts of an emulsifier, 0.05-0.2 parts of a stabilizing agent, 0.03-0.1 parts of a thickening agent, 0.1-0.55 parts of a nutrient, 0.01-0.05 parts of sodium chloride, 0-1 parts of an acidity conditioning agent, and water. The dairy product has at least one of the following advantages: abundant nutrition, good stability, and reduced incidence rate of lactose intolerance.

The present invention provides an efficient and improved process for the production and purification of high molecular weight hyaluronic acid (HA). One embodiment relates to providing growth conditions for production of high molecular weight HA in Streptococcus zooepidemicus. For example, growth in medium comprising a lower protein concentration, e.g., 1% casein hydrolyzate, and a higher sugar concentration, e.g., 5% sucrose, gave 5-6 g / L of high molecular weight HA in the range of 3.5-4.0×106 Da. The present invention also provides an efficient process for the purification of HA comprising treatment with silica gel then active carbon, and subsequent diafiltration with less volume of solvent.

The invention provides a direct-drinking type gamma-aminobutyric acid solid beverage. The direct-drinking type gamma-aminobutyric acid solid beverage consists of the following components in parts by weight of 15-30 parts of gamma-aminobutyric acid, 3-15 parts of casein hydrolysate peptides, 0.5-2 parts of anhydrous citric acid, 0.05-0.2 part of a sweetening agent, 40-55 parts of sugar alcohol, 10-23 parts of fruit powder and 2.8-4 parts of auxiliary materials. The direct-drinking type gamma-aminobutyric acid solid beverage disclosed by the invention adopts a small-specification package manner,is convenient to carry, can be directly eaten without water, is good in mouth feel, can effectively improve sleep and relieve pressure, is high in safety, free from side effects and free from pharmacophilia, and has wide crowd applicability. The product namely the direct-drinking type gamma-aminobutyric acid solid beverage disclosed by the invention has the following technical effects that the product adopts an oral cavity direct drinking type, and contains fewer proteins, so that the burden of the kidney is reduced; the product does not contain alpha-lactalbumin, so that the allergy risk ofcrowds is reduced; and besides, the effects of the products for improving sleep and relieving pressure are not influenced.

The invention discloses a tissue culture and rapid propagation method of hemionitis arifolia seedlings by virtue of a green spherical body manner. Mediums used in the tissue culture and rapid propagation method comprise an induction medium, a propagation medium and a differential medium, wherein the induction medium is prepared from 0.4-1.0mg / L 1 / 6MS-1 / 4MS and TDZ, 0.1-0.3mg / L NAA and 400-800mg / L casein hydrolysate; the propagation medium is prepared from 0.1-0.3mg / L 1 / 6MS-1 / 4MS and TDZ, 0.5-1.0mg / L 6-BA, 0.1-0.3mg / L NAA and 400-800mg / L casein hydrolysate; the differential medium is prepared from 1 / 4MS-1 / 2MS and 3-6g / L active carbon; cane sugar and agar are added into all the mediums; the pH value of each medium is 5.80. The induction rate of the green spherical body is greater than 88%; the proliferation time is 11-12.5; the differentiation rate is greater than 98%; 1.5 million to 2 million of seedlings can be propagated from one green spherical body in one year; the survival rate s greater than 95%.

The invention discloses a granular feed for juvenile fish of bostrichthys sinensis. The granular feed comprises the following components in parts by weight: 40-60 parts of animal nutritional ingredients, 20-40 parts of plant nutritional ingredients, 15-30 parts of functional ingredients, 0.3-3 parts of special vitamins, 0.3-3 parts of special trace elements, 0.2-2 parts of casein hydrolysate and 0.2-2 parts of lysine. A preparation method comprises the following steps: (1) manufacturing raw materials; (2) synthesizing a biological material; (3) converting to a cooked material; (4) puffing and granulating; (5) drying; (6) spraying and coating; and (7) performing vacuum drying. The feed disclosed by the invention adopts scientific matching and an advanced processing technology is used for assistance, so that the digestion and absorption rate of the feed is effectively improved, the growth of the juvenile fish of bostrichthys sinensis can be effectively promoted, and the occurrence of diseases during cultivation is reduced.

The invention discloses a method for rapidly breeding a hybrid orchid by a root, as well as a culture medium, and belongs to the technical field of plant tissue culture. The method comprises steps as follows: a root segment of the hybrid orchid is selected and inoculated on an induction culture medium for dark culture, and a protocorm is induced; after a large amount of the protocorms are proliferated, the protocorms are transferred for light culture and develop into tufted seedlings; and the tufted seedlings are cut into single seedlings and inoculated to a strong seedling rooting culture medium, and a rooting plant is cultured and obtained. According to the method, MS (murashige and skoog) is taken as a basic culture medium, 6-BA (6-benzyladenine), PIC (4- amino-3,5,6-trichloropyridine-2-acid) and casein hydrolysate are required to be added to the induction culture medium, and the strong seedling rooting culture medium is the MS culture medium without hormone. According to the method and the culture medium, the breeding efficiency is high, the operation is simple and convenient, and all of the protocorm inductivity, the plant rooting rate and the transplanting survival rate of root explants can reach 100%.

The present invention relates to methods for feeding of infants delivered via caesarean section and to compositions to be administered to infants delivered via caesarean section and in particular to the use of a product obtained by fermentation of milk, whey, whey protein, whey protein hydrolysate, casein, casein hydrolysate and / or lactose by lactic acid producing bacteria. Thereby it is possible to stimulate a fast colonisation of the intestinal microbiota of said infants.