The invention discloses a preparation method of joint-inspection test paper for cardiac troponin I (cTn I) and heart-type fatty acid binding protein (H-FABP). The preparation method comprises the following steps: using a membrane-spotting metal spraying device and a mixed liquid labeled by a cTn I labeled antibody and an H-FABP labeled antibody to spray metal to a pad, so as to obtain a gold pad; using a cTn I detection line coating buffer, an H-FABP detection line coating buffer and a control line coating buffer to respectively scribe on a polyvinyl chloride base plate adhered with nitrocellulose membrane, so as to obtain the polyvinyl chloride base plate spotted with the membrane; assembling a sample pad, the gold pad, the membrane spotted polyvinyl chloride base plate and absorbent paper together, and then cutting the assembled paper to obtain the detecting test paper. With adoption of the method, the detecting test paper obtained by the invention is based on the lateral flow immune chromatography technology, and can be used by manual operation and visible reading to judge whether the blood sample contains cardiac troponin I and heart-type fatty acid binding protein, so that the diagnosis is rapid and the result is accurate, and the body of the subject can be free of damage.