The invention discloses a group of DNA methylation molecular markers for lung cancer and application of the DNA methylation molecular markers in preparation of a kit for early diagnosis of lung cancer. The marker is obtained through methylation of 20 gene sequences of CDO1, SOX17, TCF21, TRIM58, ITGA9, CYYR1, CLEC14A, SLIT2, ZNF677, IRX2, ACVRL1, OSR1, ADCY8, GALNT13, HSPB6, IRX1, ITGA5, PCDH17, TBX5 and TTEX1D1. Eight of the gene sequences are methylated fingerprint genes newly found in lung cancer, and the methylated fingerprint genes comprise ADCY8, GALNT13, HSPB6, IRX1, ITGA5, PCDH17, TBX5 and TTEX1D1. On the basis of the markers, a mathematical model for lung cancer diagnosis is constructed; and the model is high in sensitivity and good in specificity, the AUC can reach 0.998, and the diagnosis effect is good. The invention also discloses a method for detecting the DNA methylation marker. The DNA methylation molecular marker disclosed by the invention has good diagnostic index characteristics, can be effectively used for lung cancer diagnosis, and has relatively high clinical use and popularization values.