Metabonomics-based early diagnosis marker for chronic obstructive pulmonary disease and application thereof

A diagnostic marker and metabolomics technology, applied in the field of COPD diagnostic markers, to achieve accurate distinction, high clinical use and promotion value

Inactive Publication Date: 2020-06-16
PEKING UNIV +1
View PDF0 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the application of liquid phase mass spectrometry metabolomics technology in COPD and acute exacerbation of COPD

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Metabonomics-based early diagnosis marker for chronic obstructive pulmonary disease and application thereof
  • Metabonomics-based early diagnosis marker for chronic obstructive pulmonary disease and application thereof
  • Metabonomics-based early diagnosis marker for chronic obstructive pulmonary disease and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Research object

[0028] The study included plasma samples from 48 patients with stable COPD and 48 patients with acute COPD from Peking University Third Hospital, and 48 healthy patients with normal lung function and no chronic heart and lung diseases. Volunteer plasma samples.

[0029] 2. Non-targeted metabolomics analysis of plasma using liquid chromatography-mass spectrometry technology

[0030] All plasma samples were centrifuged and stored in a refrigerator at -80°C. Plasma samples were taken during the research, and after sample pretreatment, non-targeted metabolomics analysis was performed using a high performance liquid chromatography-mass spectrometer to obtain the original metabolic fingerprint of the sample containing chromatographic and mass spectral information. The specific operations are as follows:

[0031] 2.1 Instruments and reagents

[0032] Experimental instruments include: high performance liquid chromatography mass spectrometry (U3000 / QEaxctive, Therm...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a metabonomics-based diagnosis marker for chronic obstructive pulmonary disease and application thereof. The diagnosis marker comprises the following 28 blood plasma metabolismmarkers: phosphatidylcholine PC 16:1-36:3, phosphatidylcholine PC 26:0-22:4, phosphatidylcholine PC 26:0-22:3, phosphatidylcholine PC 47:5e, phosphatidylcholine PC 44:11e, phosphatidylcholine PC 16:0-24:5, phosphatidylcholine PC 20:2-20:3, phosphatidylcholine PC 40:10, phosphatidylcholine PC 38:9e, phosphatidylcholine PC 18:1-20:4, phosphatidylcholine PC 16:0-20:3, phenylacetaldehyde, dihydrouracil, nicotinamide, 4-methoxycinnamic acid, ketovaline, threonine, DL-3-aminoisobutyric acid, pyruvic acid, stachyose, caffeic acid, N, N-dimethylaniline, maltotriose, D(-)-gulonate-gamma-lactone, and L-asparaginase. The marker provided by the invention has good classification on metabolome data of patients suffering chronic obstructive pulmonary disease and healthy people, and can accurately distinguish the patients suffering chronic obstructive pulmonary disease from the healthy people.

Description

[0001] The present invention claims the priority of Chinese patent application CN202010078213.1, and the contents of the description, drawings and claims of the priority document are incorporated into the specification of the invention in full and are taken as a part of the original description of the specification of the invention. The applicant further declares that the applicant has the right to modify the specification and claims of the present invention based on the priority document. Technical field [0002] The invention belongs to the field of clinical laboratory diagnosis, and specifically relates to a COPD diagnostic marker based on metabolomics and its application. Background technique [0003] Chronic Obstructive Pulmonary Disease (COPD), or chronic obstructive pulmonary disease, is an important chronic respiratory disease that seriously endangers human health. An epidemiological survey in my country shows that the prevalence of COPD among people over 40 years old is 1...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N30/02G01N30/86
CPCG01N30/02G01N30/8686G01N33/5308G01N2800/122G01N2800/7066
Inventor 尹玉新周骏拓李秋钰
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap