Metabonomics-based early diagnosis marker for chronic obstructive pulmonary disease and application thereof
A diagnostic marker and metabolomics technology, applied in the field of COPD diagnostic markers, to achieve accurate distinction, high clinical use and promotion value
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[0027] 1. Research object
[0028] The study included plasma samples from 48 patients with stable COPD and 48 patients with acute COPD from Peking University Third Hospital, and 48 healthy patients with normal lung function and no chronic heart and lung diseases. Volunteer plasma samples.
[0029] 2. Non-targeted metabolomics analysis of plasma using liquid chromatography-mass spectrometry technology
[0030] All plasma samples were centrifuged and stored in a refrigerator at -80°C. Plasma samples were taken during the research, and after sample pretreatment, non-targeted metabolomics analysis was performed using a high performance liquid chromatography-mass spectrometer to obtain the original metabolic fingerprint of the sample containing chromatographic and mass spectral information. The specific operations are as follows:
[0031] 2.1 Instruments and reagents
[0032] Experimental instruments include: high performance liquid chromatography mass spectrometry (U3000 / QEaxctive, Therm...
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