33 results about "Normal lung" patented technology

The invention discloses a formula for reducing phlegm and resolving masses, and a preparation method and application of freeze-dried powder of the formula for reducing phlegm and resolving masses, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition inhibits triple negative breast cancer adhesion-metastasis by regulating an FAK / SRC pathway, and comprises the following components by weight: 10-20 g of poria cocos, 10-20 g of rhizoma bolbostemmae, 10-20 g of radix trichosanthis, 5-12 g of curcuma zedoary, 10-20 g of selfheal, 10-20 g of oyster (smashed and decocted first), and 5-12 g of uniflower swisscentaury root. In-vitro experiments show that HTSJF can inhibit TNBC cell invasion, migration and adhesion (P is less than 0.01) in a dose-dependent manner, and along with reduction of the phosphorylation level of an FAK / SRC pathway, it is prompted that HTSJF inhibits TNBC transfer by regulating the pathway. In-vivo experiments prove that the HTSJF can obviously inhibit the reduction of TNBC pulmonary metastasis focus (P is less than 0.05) and recover part of normal pulmonary alveolar structures, and the inhibition effect of the HTSJF on TNBC pulmonary metastasis is further proved.

The invention belongs to the technical field of deep learning and image classification, and relates to a lung cancer tissue classification method based on improved Swinin-Transformer, and the method specifically comprises the following steps: S1, preparing a data set: collecting lung full-view pathological tissue slice images to construct the data set, which comprises various lung cancer subtype tissue slices and normal lung tissue slices; the method is a multi-example learning method, a self-supervised comparative learning mode is adopted to compare feature differences between positive and negative samples, a double-flow channel structure is adopted to extract features under different amplification factors, each channel takes Swim-Transform as a main network framework, an EMA module is added between Swin Transform Block modules to enhance the capability of extracting multi-scale features, and the multi-scale feature extraction efficiency is improved. And carrying out feature fusion by adopting a feature weighted aggregation mode so as to carry out classification prediction on the whole image. According to the method, the final classification result is returned to the original image and displayed by the thermodynamic diagram, so that the purposes of full-view image cancer classification and focus area detection are achieved.

The invention relates to the technical field of biology, and particularly discloses application of LLPH as a lung cancer diagnosis biomarker and a treatment target spot, an LLPH expression inhibitor is an LLPH gene knockout plasmid and a genetic engineering construct containing sgRNA and a carrier, the LLPH expression inhibitor is suitable for CRISPR / Cas system mediated gene editing, the sgRNA sequence is 5 '-CTAAAGCTTACGGAGTAAG-3', the carrier is pX459M, and the enzyme cutting site is BbsI-BbsI; by detecting the relative expression quantity of LLPH in normal lung bronchial epithelial cells and lung cancer cell lines, it is found that LLPH is highly expressed in the lung cancer cell lines, and it is shown that LLPH is closely related to lung cancer development. The invention can provide a new biomarker for diagnosis of lung cancer. Lung cancer A549 and H1299 cells are used as models, and the LLPH gene is knocked out, so that the in-vitro malignant proliferation capacity of the lung cancer cells A549 and H1299 can be remarkably inhibited, and LLPH can be used as a potential treatment target for inhibiting lung cancer proliferation.

The application provides an application of Buyang Huanwu Decoction in the preparation of a product for treating high altitude pulmonary injury of qi deficiency and blood stasis syndrome, and belongs to the technical field of medicines. After the prescription of the Buyang Huanwu Decoction is decocted with water, the decoction is used for treating high altitude pulmonary injury, can reduce pathological injury of lung tissues, reduce thickening of alveolar walls, make lung tissue structure clearer, and close to normal lung tissue morphology; meanwhile, the decoction can enhance exercise capacity, reduce asthenia symptoms of high altitude pulmonary injury of qi deficiency and blood stasis syndrome, and effectively treat high altitude pulmonary injury of qi deficiency and blood stasis syndrome.

The invention discloses a pharmaceutical composition for resisting non-small cell lung cancer as well as a preparation method and application of the pharmaceutical composition, belongs to the technical field of antitumor drugs, and mainly solves the technical problem that application is affected due to the fact that an Iridobelamal A compound has high toxicity to normal lung cells. The composition provided by the invention comprises the following active components: an iridoaldehyde triterpenoid compound Iridobelamal A and a flavonoid compound tectorigenin, the molar ratio of the Iridobelamal A to the tectorigenin is 1: 2-1: 5, the molecular formula of the Iridobelamal A is C30H50O4, the molecular formula of the tectorigenin is C16H12O6, and the composition and a pharmaceutical carrier are prepared into an oral preparation or an injection. The composition is used for preparing a medicine for treating non-small cell lung cancer by neutralizing triterpene toxicity and synergistically enhancing anti-tumor activity.

This invention provides the application of N-Methylatanine in the preparation of fungal inhibitors, belonging to the field of antifungal drug technology. The N-Methylatanine of this invention is of natural origin and has low toxicity, exhibiting IC50 activity against normal human lung epithelial cells. 50 The concentration is >40 μM; it exhibits significant antibacterial effects. At a concentration of 100 μM, its inhibition rates against *Epidermophyton floccosum*, *Trichophyton rubrum*, and *Microsporum gypseum* reached 99.385±0.8%, 102.17±1.956%, and 100±0.115%, respectively. This invention provides a new approach for the treatment of fungal infections of the skin in humans and animals.

The invention discloses a benzacridine-3, 4-dicarboximide derivative as well as a preparation method and application thereof, the structure of the benzacridine-3, 4-dicarboximide derivative is shown as I-A, and n is equal to 1, 2, 3 or 4; the invention also discloses hydrochloride of the benzacridine-3, 4-dicarboximide derivative and a preparation method, all the compounds are subjected to CCK-8 experiments to investigate the cytotoxicity to 14 tumor cells and human normal lung embryo fibroblasts, and the compound 4 with the best activity is used for evaluating the cytotoxicity to SK-OV-3 ovarian cancer cell proliferation and invasion, the cytotoxicity to SK-OV-3 ovarian cancer cells, the cytotoxicity to human normal lung embryo fibroblasts and the cytotoxicity to SK-OV-3 ovarian cancer cells and the cytotoxicity to human normal lung embryo fibroblasts. And influence of migration capability. Subsequently, in-vivo anti-tumor activity of the compound 4 is found in a nude mouse xenotransplantation tumor experiment, and whether the compound has toxicity to mice or not is observed through pathological tissue slices under the administration treatment concentration. The invention has great value in discovery of new anti-tumor small molecule drugs and research and development of clinical drugs thereof.

The invention relates to the technical field of image analysis, in particular to a dynamic image registration method for pulmonary atelectasis focus volume change monitoring. Firstly, a CT image sequence of a respiratory cycle of a patient is collected; determining a breathing gray level change value based on the gray level difference of the pixel points between the images; dividing a local area according to the position of a pixel point and gray scale information; in a respiratory cycle, gas exchange in a pulmonary atelectasis area is limited, expansion capacity during inspiration is limited, a dynamic mode different from a normal lung tissue is formed, a first abnormal factor is determined by analyzing gray level change relation of pixel points in a local area and a global area and combining a respiratory gray level change value, and an abnormal area is screened out; due to pulmonary atelectasis, lung blood vessel displacement and gathering and lung volume reduction are caused, pixel point number difference characteristics are analyzed, and a second abnormal factor is determined by integrating local region blood vessels, a distribution relation and a breathing gray level change value; and finally, acquiring an adaptive enhancement factor based on the two, and performing adaptive sharpening enhancement on the image to improve the subsequent registration precision.

The invention discloses a human SLC7A11AR gene. The nucleotide sequence of the human SLC7A11AR gene is as shown in SEQ ID NO: 1; the reagent for detecting the expression quantity of the human SLC7A11AR gene is applied to preparation of the lung adenocarcinoma clinical diagnosis reagent, the expression level of the human SLC7A11AR gene is in negative correlation with clinical prognosis of the lung adenocarcinoma, and experimental results show that the expression of the human SLC7A11AR gene in a lung adenocarcinoma cell line is higher than that of normal lung epithelial cells; after the SLC7A11AR gene is knocked down, the proliferation of the lung adenocarcinoma cell line is obviously inhibited; the ASO sequence for inhibiting the expression of the human SLC7A11AR gene is combined with a ferroptosis inducer, so that the growth of lung adenocarcinoma in vivo can be obviously inhibited; the invention reveals that the SLC7A11AR gene is a potential risk gene of lung adenocarcinoma, and SLC7A11AR expression inhibition is combined with a ferroptosis inducer IKE to enhance the treatment effect of lung adenocarcinoma.

The present application relates to the field of biological extraction, and particularly relates to a kind of rattan tea selenium flavone III and its extraction method and application, the method includes boiling water extraction, macroporous resin purification, normal phase chromatography impurity removal, gel chromatography column chromatography impurity removal process, finally concentrated, spray drying treatment, as refined rattan tea selenium flavone III.The present application has simple process, low production cost, high rattan tea selenium polysaccharide IV compound yield and purity, the rattan tea selenium flavone III prepared has excellent anticancer activity to cancer cell, and also has no toxic side effects to normal lung epithelial cells, can be made into any dosage form acceptable in food and pharmacy.

The invention belongs to the technical field of biological medicine, and particularly relates to application of a CCL20 / CCR6 axis in bronchial pulmonary dysplasia. According to the application of the targeted CCL20 / CCR6 shaft in the aspect of preparing the medicine for treating bronchial pulmonary dysplasia, it is proved that CCL20 can cause damage to the normal pulmonary development process of newborn mice; and the neutralizing antibody of CCL20 or the antagonist of CCR6 can effectively improve the pulmonary alveoli development retardation of the BPD mouse induced by high oxygen exposure. The invention provides a new thought for preparing the medicine for treating the BPD, and a method for better treating the BPD is realized by applying the targeted CCL20 / CCR6 to the development process of the medicine related to the BPD; meanwhile, the selection field of medicines for treating BPD is widened, and contributions are made to the development of the technical field.

The invention discloses a podophyllotoxin structure-based tubulin / EGFR (epidermal growth factor receptor) dual-targeting podophyllotoxin triazole derivative and application thereof in antitumor drugs, and belongs to the technical field of medicinal chemistry. A triazole group is introduced into a podophyllotoxin mother nucleus through click chemistry, and a series of novel derivatives are designed and synthesized. Molecular docking studies show that the compound can target tubulin and EGFR kinase structural domains at the same time, and tumor cell proliferation is inhibited through a double-target synergistic effect. In-vitro experiments prove that the derivative has significant inhibitory activity on non-small cell lung cancer cells and has low toxicity on normal pulmonary epithelial cells. The dual-targeting strategy can overcome the drug resistance problem of the EGFR inhibitor, optimizes the pharmacokinetic characteristics, has the advantages of high oral availability, good in-vivo stability and the like, and is suitable for treatment of solid tumors such as non-small cell lung cancer and the like.

The invention relates to the technical field of biological medicine, in particular to a nucleic acid aptamer specifically combined with lung cancer cells and a screening method and application of the nucleic acid aptamer. A cell SELEX screening method is improved, lung cancer cells are used as positive screening cells, normal lung epithelial cells are used as negative screening cells for screening, the screening pressure is increased round by round, the screening efficiency is improved, and the nucleic acid aptamers Apt-1 and Apt-2 with high specificity and affinity for the lung cancer cells are obtained. As a novel molecular recognition probe, the nucleic acid aptamer disclosed by the invention has a wide application prospect in the aspects of early diagnosis, targeted therapy and the like of lung cancer.

The invention relates to the technical field of personal health risk assessment, in particular to a pulmonary nodule operation intervention assessment system based on image analysis. The method comprises the following steps: firstly, acquiring CT images of the lung of a patient in different preoperative periods and all nodules in each CT image; all nodules are classified and pre-classified into solid nodules, ground glass nodules and suspected partial solid nodules; further classifying all suspected partial solid nodules into partial solid nodules and ground glass nodules; and finally marking the operation risk according to the classification result of the nodules. According to the method, on the basis of the texture distinguishing features and the growth rule features of different types of nodules in the CT image, the misjudgment risk of the ground glass nodules and part of solid nodules growing on a normal lung tissue structure is reduced, so that the nodules are accurately classified, and the operation intervention necessity is accurately evaluated.

The sleeve type thoracic cavity puncture needle comprises a puncture needle body and a hollow tube, the puncture needle body is placed in the hollow tube, the upper end of the puncture needle body is fixedly connected with a connector, the interior of the connector is fixedly connected with a whistle tube, the whistle tube is aligned with the center of the puncture needle body, and a whistle hole is formed in the side surface of the whistle tube. A vibration piece is arranged on one side of the whistle hole and fixedly connected with the inner wall of the whistle pipe, the puncture needle is sleeved with a sleeve, the upper end of the sleeve is fixedly connected with a sleeve head, the sleeve head is buckled below the connector, the upper end of the hollow pipe is buckled with a pipe cap, the side surface of the pipe cap is fixedly connected with a hanging plate, and the upper end of the pipe cap is fixedly connected with a plastic plate. The problem that when an existing sleeve type thoracocentesis needle is used in an emergency, pneumothorax cannot be rapidly and accurately treated is solved, and meanwhile the situation that normal lungs and other organ tissues of a patient are injured due to the fact that medical staff puncture too deep in the operation process is avoided.

This invention relates to a method for extracting compound Alisma alkaloid A from Alisma plantago-aquatica and its application, effectively solving the problem of extracting Alisma alkaloids from Alisma plantago-aquatica and enabling its application in the preparation of drugs for treating lung cancer. This compound, Alisma alkaloid A, significantly reduces cell viability and Bcl2 expression, induces apoptosis and mitochondrial membrane depolarization in A549 lung cancer cells, and significantly increases the expression of Bax, Caspase3, and Caspase9, without affecting normal human lung epithelial cells BEAS-2B. It has the effect of inducing apoptosis in A549 cells through the mitochondrial apoptosis pathway, thus realizing the application of Alisma alkaloid A in the preparation of drugs for treating lung cancer. The method of this invention is scientifically sound, novel, unique, and easy to operate, effectively extracting compound Alisma alkaloid A from Alisma plantago-aquatica. This compound has the effect of inducing apoptosis in A549 cells through the mitochondrial apoptosis pathway, realizing the application of Alisma alkaloid A in the preparation of drugs for treating lung cancer, and expanding the medicinal and commercial value of Alisma plantago-aquatica.

The invention relates to application of ulinastatin and tetrandrine in preparation of a medicine for preventing and / or treating radiation-induced lung injury, and belongs to the technical field of biological medicine. The invention provides application of ulinastatin combined with tetrandrine in preparation of a medicine for preventing and / or treating radiation-induced lung injury. The ulinastatin and the tetrandrine are combined to be used as a medicine for treating mice suffering from radiation-induced lung injury, and the result shows that the ulinastatin and the tetrandrine have very good treatment and prevention effects on the radiation-induced lung injury, and the ulinastatin and the tetrandrine are non-toxic to lung cells; growth and development of normal lung cells cannot be inhibited, the treatment effect does not have dose dependence, the content of pro-inflammatory cytokines TNF-alpha and IL-6 can be remarkably reduced, the symptom of lung tissue inflammation can be relieved, and the survival rate of mice suffering from radiation-induced lung injury can be increased.

This invention discloses a stable human lung cell line overexpressing MLT1L, its construction method, and its applications. This invention belongs to the field of biomedicine, specifically relating to a stable human lung cell line overexpressing MLT1L, its construction method, and its applications. The substance promoting MLT1L expression of this invention can be applied to: (A1) improving the migration ability of normal lung cells; (A2) preparing products for improving the migration ability of normal lung cells; (A3) improving the invasive ability of normal lung cells; (A4) preparing products for improving the invasive ability of normal lung cells; wherein MLT1L is a long non-coding RNA with a nucleotide sequence as shown in SEQ ID No:1. This invention provides a method for constructing a stable human lung cell line overexpressing MLT1L, and overexpression of MLT1L-2034 can significantly increase the viability, proliferation, and invasive ability of Beas-2B cells.

The present application relates to the technical field of image analysis, in particular to a dynamic image registration method for monitoring volume change of atelectasis lesion. First, a CT image sequence of a patient in one respiratory cycle is collected; a respiratory gray level change value is determined based on the gray level difference of the pixels between images; then, local areas are divided according to the position and gray level information of the pixels; in the respiratory cycle, the gas exchange of the atelectasis area is limited, the expansion capacity is limited during inhalation, and a dynamic mode different from normal lung tissue is formed; by analyzing the gray level change relationship of the pixels in local and global areas, and combining the respiratory gray level change value, a first abnormal factor is determined to screen out abnormal areas; because the atelectasis causes the displacement and gathering of pulmonary blood vessels and the reduction of lung volume, the difference characteristics of the number of pixels are analyzed, and the second abnormal factor is determined by comprehensively considering the blood vessels in the local area, the distribution relationship and the respiratory gray level change value; finally, an adaptive enhancement factor is obtained based on the two factors, the image is adaptively sharpened and enhanced, and the subsequent registration accuracy is improved.

The invention discloses an early warning evaluation method based on canceration caused by pulmonary nodule immune microenvironment imbalance, which comprises the following steps: S1, acquiring autoantibody detection data of normal, pulmonary nodule and lung cancer people from a database, and establishing a pulmonary nodule canceration data set Panne l containing 159 autoantibodies as risk early warning factors of pulmonary nodule canceration; s2, preparing a lung cancer risk factor Panne l protein chip according to the Panne l data, wherein the lung cancer risk factor Panne l protein chip comprises antigen proteins of 159 lung cancer related autoantibodies; s3, using a Panne l protein chip to detect the serum of the crowd; and S4, according to the detection data, establishing a risk early warning model of pulmonary nodule canceration for the analysis and early warning of the lung cancer occurrence risk of the crowd (mainly aiming at the pulmonary nodule crowd). According to the method, the cancer related autoantibodies are systematically detected through a protein chip method for people with high detection rate of pulmonary nodules in China, and the method is used for early warning of the occurrence risk of pulmonary nodule immune abnormality canceration of people.

The present application discloses a sputum obstruction detection method, system, terminal and medium based on a lung binary tree model. The sputum obstruction detection method based on a lung binary tree model includes: obtaining a normal lung impedance curve of a subject under normal lung conditions; establishing a lung binary tree model, calibrating the parameters of the lung binary tree model according to the normal lung impedance curve, and obtaining normal values ​​of airway geometric parameters; obtaining the current lung impedance curve of the subject during a forced oscillation test when detecting sputum accumulation, and determining the location and degree of sputum obstruction of the subject based on the current lung impedance curve, the normal lung impedance curve and the normal values ​​of airway geometric parameters. The present application simplifies the lung binary tree model, quantitatively analyzes the accumulation of sputum in the lungs, and can accurately identify the location and degree of sputum obstruction. The forced oscillation measurement is used to improve the patient's comfort, thereby reducing the physical and psychological burden of sputum suction on the patient.

The invention discloses TERC promoter methylation and application thereof in non-small cell lung cancer diagnosis and prognosis, and relates to the technical field of biological medicine. It is found that the methylation level of the TERC promoter is reduced in non-small cell lung cancer tissues, TERC methylation modification is remarkably changed in most of the non-small cell lung cancer tissues, and universal detection of tumors in different stages can be achieved; the low methylation state of a key site in a promoter region is specifically captured, so that cancer tissues and normal lung tissues are effectively distinguished, and a reliable basis is provided for clinical definite diagnosis. The kit for detecting the methylation of the DNA sequence of the TERC promoter can specifically detect the DNA methylation level of the TERC promoter, and can also specifically and quantitatively detect the DNA methylation level of the TERC promoter; the detection result can provide an evaluation method for the condition and individualized medication monitoring of non-small cell lung cancer patients, the early diagnosis of tumors, and the prognosis detection of the patients on drug therapy.

The invention discloses a non-small cell lung cancer specific promoter gene element and a screening method and application thereof, and belongs to the technical field of gene engineering. A non-small cell lung cancer specific promoter gene element to be screened is obtained through a biological database, then the non-small cell lung cancer specific promoter gene element to be screened, a reporter gene and an expression vector are fused, and a non-small cell lung cancer specific promoter region in the expression vector regulates downstream reporter gene expression. The non-small cell lung cancer specific promoter gene element KRT19P4 is screened by taking the biological activity of a reporter gene as an activity evaluation standard of a non-small cell lung cancer biomarker promoter, and the sequence is shown as SEQ ID NO.16. A bioluminescence test proves that the KRT19P4 promoter gene element can enable luciferase reporter genes in normal lung cells and non-small cell lung cancer cells to generate differential enzyme activity intensity.

The invention provides an application of N-Methylalline in preparation of a fungus inhibitor, and belongs to the technical field of antifungal drugs. According to the invention, the N-Methylalanine is a natural source and has small toxicity, and the IC50 of the N-Methylalanine to human normal pulmonary epithelial cells is greater than 40 [mu] M; the antibacterial effect is obvious, and under the concentration of 100 [mu] M, the inhibition rates on epidermophyton flocculent, trichophyton rubrum and microsporum gypsum reach 99.385 + / -0.8%, 102.17 + / -1.956% and 100 + / -0.115% respectively. The invention provides a new way for treatment of human and animal epidermis fungal infection.

The present invention relates to a lung ex vivo model, a construction method therefor, and a use thereof. The lung ex vivo model provided by the present invention uses lung organoids instead of traditional cell lines to construct the lung ex vivo model. The construction method provided by the present invention involves sequentially seeding lung organoids, endothelial cells, macrophages, and, optionally, immune cells. A normal lung ex vivo model is obtained by first culturing in a liquid-liquid mode, followed by culturing in an air-liquid mode. On this basis, a lung fibrosis model can optionally be obtained by treating the lung ex vivo model by using a lung fibrosis inducer.

The invention relates to a super-selection lung recruitment system and a recruitment path generation method thereof. The system comprises an EIT monitoring subsystem, a CT image acquisition subsystem, a multi-modal image fusion subsystem, a control subsystem and a lung recruitment subsystem. The EIT monitoring subsystem acquires and processes lung electrical impedance data in real time and generates an EIT image marking a collapse area, the CT image acquisition subsystem acquires chest CT scanning data, the multi-modal image fusion subsystem calculates and outputs an optimal path by combining the two types of data, the control subsystem receives the optimal path data and converts the optimal path data into a control instruction, and the control subsystem controls the control instruction. A data adjusting instruction is fed back in real time according to the EIT monitoring subsystem, and finally the lung recruitment subsystem reaches the lung collapse area along the optimal path according to the instruction to complete recruitment. Compared with the prior art, the method has the advantages that accurate positioning and super-selection re-expanding of the lung collapse area are achieved, the re-expanding accuracy and efficiency are improved, interference on normal lung tissue is reduced, and the operation risk is reduced.

The invention provides preparation of a foaming hydrogel lung lobe model and a surgical thoracoscope training model. Gel materials polyethylene glycol 2000 and polyethylene glycol 4000 which are soft, elastic and pollution-free and have physical properties close to those of human tissues and organs are adopted as a main body, so that the texture of a normal lung lobe is accurately re-engraved; meanwhile, the hardness of the model is adjusted by finely adjusting the thickness and hardness of the material, namely adjusting the ratio of the foaming accelerant to the fluffy and soft aid, and the larger the ratio is, the harder the material is, so that the lung lobe swelling phenomenon caused by inflammation can be simulated; besides, by adjusting the physical property, namely the hardness in the mechanical property, of the material, the necessary flexibility is kept to adapt to the natural form of the lung lobe, and a nodule-shaped structure with increased hardness can be formed in a specific area, so that the touch sense and the form of a cancer focus are accurately simulated.

This invention discloses the preparation and application of organoselenium flavonoids with high antioxidant activity and low toxicity, relating to the field of functional food technology. The method of this invention includes pulverizing selenium-enriched vine tea, selenium-enriched kudzu root, selenium-enriched gastrodia elata, and selenium-enriched papaya, then mixing them according to a specified ratio, low-temperature reflux extraction, hollow fiber ultrafiltration membrane purification, activated carbon depigmentation, reversed-phase silica gel degreasing and polysaccharide removal, gel chromatography purification, and freeze-drying or spray drying. This invention uses a novel food resource combination of vine tea, kudzu root, gastrodia elata, and papaya—medicinal and edible herbs—as raw materials to extract organoselenium flavonoids with a high yield. Furthermore, the extracted selenium flavonoids exhibit extremely strong DPPH free radical scavenging activity and show no toxic side effects on normal human renal tubular epithelial cells (HKC) and normal lung epithelial cells (BEAS-2B). Therefore, the content of this invention is of great significance to the development of the field of antioxidant functional foods.

The invention relates to the technical field of medical instruments, in particular to a lung lobe ventilation catheter for an ARDS patient, which comprises a main body tube, a first cavity and a second cavity are arranged at two ends of the main body tube in a penetrating manner, a main ventilation tube is fixedly mounted in the first cavity, and a first lung lobe ventilation tube is inserted in the second cavity; one end of the main breather pipe is provided with a main body connector, one end of each first lung lobe breather pipe is provided with a branch body connector, and the tail ends of the two first lung lobe breather pipes and the tail end of the main breather pipe are connected with visual devices with cameras. Ventilation is conducted in the main ventilation pipe or the first lung lobe ventilation pipe, ventilation and ventilation parameter setting of different lung lobes are achieved, personalized ventilation parameter setting better conforming to pathophysiological characteristics can be conducted on lesion lung lobes on the premise that normal lung lobe ventilation is not affected, then volume injuries and shear injuries are reduced, and the ventilation efficiency is improved. The occurrence rate of the breathing machine related lung injury is reduced.

The invention provides a pulmonary surfactant protein D antibody or an antigen binding fragment, the antibody is a monoclonal antibody generated by a hybridoma, and the antigen binding fragment is an antigen binding fragment of the monoclonal antibody generated by the hybridoma. The method can be used for determining whether a subject has a normal pulmonary surfactant protein D level or not, determining whether the subject has potential lung injury or not and determining disease progression of the subject, and has important clinical significance.