The present invention is related to an identification and / or quantification method of a biological (micro)
organism or part of it (possibly present in a biological sample) by a detection of its
nucleotide sequence among at least 4 other
homologous sequences and comprising the steps of: possibly extracting original
nucleotide sequences (1) from the (micro)
organism; amplifying or
copying with a unique pair of primer(s), at least part of original
nucleotide sequences (1) into target nucleotide sequences (2) to be detected; possibly
labelling said target nucleotide sequences (2); putting into contact the labelled target nucleotide sequences (2) with single stranded capture nucleotide sequences (3) bound by a single predetermined link to an insoluble
solid support (4), preferably a non porous
solid support, discriminating the binding of a target nucleotide sequence (2) specific of an
organism or part of it by detecting, quantifying and / or recording a
signal resulting from a hybridization by complementary base
pairing between the target nucleotide sequence (2) and its corresponding capture nucleotide sequence (3), wherein said capture nucleotide sequence (3) being bound to the insoluble
solid support (4) at a specific location according to an array, said array having a density of at least 4 different bound single stranded capture nucleotide sequences / cm2 of solid
support surface and wherein the binding between the target nucleotide sequence and its corresponding capture nucleotide sequence forms (will result in) said
signal at the expected location, the detection of a single
signal allowing a discrimination of the target nucleotide sequence specific of an organism or part of it from homologous nucleotide sequences.