152 results about "Homologous Sequences" patented technology

The invention relates to a gene GmHB1a participating in development of vascular bundles of soybean root nodules and application of the gene GmHB1a. Specifically, the invention relates to a separated gene participating in development of vascular bundles of soybean root nodules or regulation and control of the number of soybean nodules, and a coding region sequence of the gene is shown as SEQ ID NO: 1 or a homologous sequence of the gene. The invention also provides a method for regulating and controlling the nodulation number of the plant, which comprises the following steps: introducing the gene or a sequence for inhibiting the expression of the gene into a target plant cell or tissue to obtain a transgenic plant, the nodulation number of the transgenic plant is changed compared with that of the target plant, the plant is a leguminous plant, preferably soybean, and the nodulation number of the transgenic plant is changed. Optionally, the change in number of nodules is manifested as an increase in number of nodules and / or in number of single nodules, and optionally, the change in number of nodules is manifested as a decrease in number of nodules and / or in number of single nodules.

The invention relates to a gene GmHB21 participating in development of vascular bundles of soybean root nodules and application of the gene GmHB21. Specifically, the invention relates to a separated gene participating in development of vascular bundles of soybean root nodules or regulation and control of the number of soybean nodules, and a coding region sequence of the gene is shown as SEQ ID NO: 1 or a homologous sequence of the gene. The invention also provides a method for regulating and controlling the nodulation number of the plant, the method comprises the following steps: introducing the gene or a sequence for inhibiting the expression of the gene into a target plant cell or tissue to obtain a transgenic plant, the nodulation number of the transgenic plant is changed compared with that of the target plant, and the plant is a leguminous plant, preferably soybean. In addition, the invention also provides application of the gene or a sequence for inhibiting expression of the gene in cultivation of leguminous plants with changed nodulation number, such as soybean.

The invention discloses an L-aspartic acid transaminase mutant and application thereof. The L-aspartic acid transaminase mutant is obtained by performing single mutation on the 104th amino acid of the amino acid sequence of L-aspartic acid transaminase. The L-aspartic acid transaminase mutant is used for synthesizing an L-phenylglycine derivative. The L-aspartic acid transaminase mutant is from escherichia coli, homologous sequence analysis and molecular docking simulation are carried out, site-specific mutagenesis verification is combined, and single-point mutation is carried out on the 104 amino acid to obtain the T104S mutant. The L-aspartic acid transaminase mutant disclosed by the invention can be used for efficiently catalyzing to generate an L-phenylglycine derivative, and compared with a wild type, the specific activity of the T104S mutant to o-chlorophenoxyacetic acid is 3.2 times that of the wild type.

The invention belongs to the technical field of plant genetic engineering, and relates to medicago sativa polygalacturonase MsPG2, a coding gene thereof and application of the medicago sativa polygalacturonase MsPG2 in plant salt stress resistance, and an amino acid sequence of the polygalacturonase MsPG2 is shown as SEQ ID NO.1 or a derivative sequence or a homologous sequence of the amino acid sequence shown as SEQ ID NO.1. The invention further discloses a preparation method of the medicago sativa polygalacturonase MsPG2. The invention also provides a coding gene of the polygalacturonase MsPG2, wherein the nucleotide sequence of the coding gene is shown as SEQ ID NO. 2; the coding gene can be expressed by salt induction. According to the invention, the cultivation of the MsPG2 transgenic plant is realized by utilizing a genetic engineering technology, the salt tolerance of the plant is obviously improved, an important theoretical basis is provided for the cultivation and breeding work of a new salt-tolerant variety of medicago sativa, and the MsPG2 transgenic plant has a great application value.

The invention relates to a multiplex PCR (Polymerase Chain Reaction) primer probe combination, a kit and a detection method for human red blood cell RHCE genetic typing detection, and belongs to the field of biomedicine clinical molecular detection. The invention provides a multiplex PCR (Polymerase Chain Reaction) primer probe combination for human red blood cell RHCE genetic typing detection, and further provides a kit containing the multiplex PCR primer probe combination. The method has the technical advantages that the RHCE gene variation site specific primer and probe are designed through an improved ARMS technology and a homologous sequence specific base method, so that higher specificity and accuracy are realized; only four-hole reaction is needed, detection of RHC, RHc, RHE, RHe, CW- / CW +, CX- / CX +, V-VS- / V + VS + and V + / V-can be completed at a time, and higher flux and efficiency are achieved; according to the method, multiple fluorescent PCR is combined with a Taqman probe technology, qualitative typing detection is carried out on the human red blood cell RHCE gene, and compared with a traditional method, higher sensitivity, higher experiment speed, higher accuracy and a wider application range are shown.

The invention relates to a gene GmPMT for regulating and controlling soybean root nodule symbiotic nitrogen fixation and application of the gene GmPMT. Specifically, the invention relates to a separated gene for regulating and controlling soybean root nodule symbiotic nitrogen fixation or nodulation number, a coding region sequence of the gene is shown as SEQ ID NO: 1, or a homologous sequence of the gene has substitution, deletion and / or insertion of one or more nucleotides compared with the sequence shown as SEQ ID NO: 1. The invention also provides application of the gene in cultivation of soybean with increased yield or changed nodulation number, optionally, the nodulation number change shows that the nodulation number, the nodulation number per root and / or the nodulation number per unit root length are increased, and optionally, the nodulation number change shows that the nodulation number per unit root length is increased. The change of the number of nodules is manifested as the number of nodules, the number of nodules per root and / or the number of nodules per unit root length is reduced. The invention has great theoretical and application values for adjusting the symbiotic nitrogen fixation or nodule number of soybean root nodules and related application research.

The invention relates to the technical field of drug target prediction, and discloses a drug target intelligent prediction method and system, and the method comprises the following steps: 1, obtaining the structure data and sequence data of a target protein, and the homologous sequence data of the target protein; 2, performing molecular dynamics simulation according to the structural data, and calculating to obtain a dynamic conformation feature vector; according to the sequence data, an evolutionary information feature vector is obtained through calculation; analyzing the structural data to obtain an interaction feature vector; 3, obtaining an enhanced dynamic conformation feature vector, an enhanced evolutionary information feature vector and an enhanced interaction feature vector through an attention fusion mechanism, respectively obtaining importance weights through a gating fusion mechanism, and obtaining a fusion feature vector by adopting element-by-element multiplication and combining the importance weights; and 4, inputting the fusion feature vector into a graph neural network prediction model to obtain a target druggability probability.

The invention discloses a Cas12 protein and an application thereof. The Cas12 protein has an amino acid sequence which has at least 90% sequence identity with a sequence as shown in SEQ ID NO: 1 or a homologous sequence of the sequence as shown in SEQ ID NO: 1, and the amino acid sequence of the Cas12 protein is not SEQ ID NO: 1. The Cas12 protein has excellent thermal stability and trans-cleavage activity, storage and transportation of the Cas12 protein in practical application are facilitated, application scenes of the Cas12 protein and a CRISPR (clustered regularly interspaced short palindromic repeats) system based on the Cas12 protein are increased, and more choices are provided for users; in addition, on the basis of the existing protein engineering theory, the Cas12 protein mutant with improved thermal stability can also be used as a template / starting point sequence of subsequent protein engineering.

The invention belongs to a second-generation sequencing data analysis technology in the field of bioinformatics, and particularly relates to a method for classifying adeno-associated virus data on multiple reference genomes based on second-generation sequencing. The method comprises the following steps: S1, constructing a position information file of a homologous sequence on a genome; s2, establishing an original BAM file; s3, constructing a comparison information summary list T; s4, a QNAME list is constructed; s5, filtering; and S6, counting. According to the method disclosed by the invention, the main components of AAV are identified and analyzed on the computer algorithm level through a short fragment sequencing method for the first time. Compared with an experiment-based method, the analysis result of the method has higher component quantitative accuracy and lower manual operation cost. The method provided by the invention is of great significance to clinical detection of AAV drugs.

The present invention relates to the field of biotechnology and discloses a recombinant human elastin peptide with anti-aging efficacy and its application in a composition thereof. The recombinant human elastin peptide comprises the elastin peptide amino acid sequence SEQ ID: 1 or a fragment thereof, wherein the fragment comprises 30% of SEQ ID: 1, or a fragment comprises more than 30% of SEQ ID: 1, or a mutant of the above sequence. The recombinant human elastin peptide can be directed to synthesize a protein of a highly effective fragment, with a 100% human homologous sequence, while being low-cost, easy to mass-produce, avoiding animal sources, and avoiding viral risks. This allows the recombinant human elastin peptide to be safely used in cosmetics as an anti-aging and anti-photoaging ingredient, while also providing support for its application in the field of biomedical materials.

The present application relates to a gene GmPMT for regulating soybean root nodule symbiotic nitrogen fixation and application thereof. Specifically, the present application relates to an isolated gene for regulating soybean root nodule symbiotic nitrogen fixation or nodule number, the coding region sequence of the gene is shown as SEQ ID NO:1 or a homologous sequence thereof has one or more substitutions, deletions and / or insertions of nucleotides compared with the sequence shown as SEQ ID NO:1. The present application also provides the use of the gene as described above in breeding soybeans with increased yield or changed nodule number, optionally, the changed nodule number is manifested as increased nodule number, nodule number per root and / or nodule number per unit root length, optionally, the changed nodule number is manifested as decreased nodule number, nodule number per root and / or nodule number per unit root length. The present application has great theoretical and application value for regulating soybean root nodule symbiotic nitrogen fixation or nodule number and its related application research.

The application discloses a PCR primer pair and a molecular marker probe for distinguishing between barnyard grass and rice, a detection kit and application thereof. After align analysis on chloroplast genome sequences of the barnyard grass and the rice, it is found that a difference site exists in a 91 kb region, sequences of the upstream and downstream of the difference site are taken as reference sequences to select homologous sequences to design primers for PCR amplification, and the amplification result proves that the difference site fragment obtained by chloroplast genome sequence analysis exists and can be amplified and identified subsequently; the PCR amplification product is sequenced, and the sequencing result is compared to find that a 12 bp insertion site exists in the site and can separate the barnyard grass from the rice, a colloidal gold probe is designed according to the insertion site, and a RPA detection kit and a detection method are established, and the RPA detection kit is used to detect the barnyard grass and the rice, and the barnyard grass and the rice can be accurately distinguished.

The invention relates to the technical field of gene engineering, and provides application of polynucleotide, protein and a biological material in regulation and control of plant nutrient substance storage organ development. The polynucleotide has a sequence as shown in SEQ ID NO: 1; or a complementary sequence, a degenerate sequence or a homologous sequence thereof; or polynucleotide hybridized with the nucleotide sequence as shown in SEQ ID NO: 1 under strict conditions or a complementary sequence of the polynucleotide. The polynucleotide is related to the development of plant nutrient substance storage organs, promotes the development of the plant nutrient substance storage organs through overexpression, and can be used for cultivating plant varieties.

The present invention provides a crop genome gene prediction method based on co-evolution feature analysis. The method constructs a multi-species homologous sequence data set, calculates weighted mutual information values, establishes a co-evolution feature network, and combines network topology features and sequence conservation features to use a deep learning model to predict potential coding regions. The method of the present invention integrates sequence alignment quality scoring, adaptive network pruning and multi-level feature analysis, thereby improving the accuracy and reliability of gene prediction in crop genomes. The method can be used to identify promoter regions, exon regions and terminator regions, providing an effective tool for crop genome annotation.

A homologous recombination method of excising a specific DNA sequence from a DNA molecule. The method includes providing the DNA molecule including a pair of target sequences that are a target of a site-specific recombination enzyme and a pair of homologous sequences that are DNA sequences sharing homology with each other. The pair of target sequences are oriented in different directions from each other and arranged on opposite sides of the specific DNA sequence. The pair of homologous sequences are arranged on opposite sides of the specific DNA sequence at positions further outside of the pair of target sequences. The method further includes inducing homologous recombination between the pair of homologous sequences by action of the site-specific recombination enzyme on the DNA molecule so that the specific DNA sequence and the pair of target sequences are excised from the DNA molecule.

The present invention relates to a modified MARCH gene, the wildtype of which comprises SEQ ID No. 1 and SEQ ID No. 3, or comprises a homologous sequence having at least 70% sequence identity to SEQ ID No. 1 and / or SEQ ID No. 3, which modified gene leads to resistance to downy mildew. The modification may leads to an altered gene expression, such as a reduced gene expression. In particular, the modified MARCH gene comprises a modification in SEQ ID No. 3, or in a homologous sequence having at least 70% sequence identity to SEQ ID No. 3. In one embodiment, the modification is on position 77 of SEQ ID No. 3, or on the corresponding position of a homologous sequence having at least 70% sequence identity. In one embodiment, the modified MARCH gene comprises SEQ ID No. 4. The invention further relates to a polynucleotide comprising a modified MARCH gene and a modified Malectin-like-RLK gene, preferably comprising SEQ ID No. 4 and SEQ ID No. 7. The invention further relates to a plant comprising a modified MARCH gene and to a marker for the identification of a modified MARCH gene.

The present invention relates to a construct comprising a cell-penetrating peptide sequence coupled by a peptide bond to at least one first linker molecule which is coupled by a peptide bond to the N-terminal end of an interfering amino acid sequence selected from the following sequences: SEQ ID NO:1 which forms the 2104-2206 domain of the human FlnA protein and SEQ ID NO:2 which defines the 330-350 domain of the UT receptor, the sequences having at least 90% identity and, preferably, at least 95% identity with the aforementioned sequences and sequences substantially homologous to the aforementioned sequences and preferably derived from the latter by one or more conservative substitutions.

The invention discloses a Chinese cabbage clubroot-resistant gene BrRLP31 and application of the Chinese cabbage clubroot-resistant gene BrRLP31. The nucleotide sequence of the Chinese cabbage clubroot-resistant gene BrRLP31 is as shown in SEQ ID No. 1. Functional verification results show that after the expression of the endogenous BrRLP31 of the Chinese cabbage is inhibited by utilizing a VIGS technology, the resistance of the plant to clubroot is obviously reduced, and the disease index and the root swelling severity of the plant are obviously higher than those of a control material. On the other hand, the transgenic flowering cabbage plant over-expressed by the BrRLP31 is constructed by an agrobacterium-mediated method, and disease resistance identification proves that the heterologous over-expression of the BrRLP31 obviously improves the resistance of the plant to plasmodiophora brassicae. The results show that the BrRLP31 gene plays a key positive regulation role in the clubroot resistance process of the Chinese cabbage, the disease resistance of cruciferae crops and other horticultural plants is improved by utilizing the gene and the homologous sequence thereof, and the gene and the homologous sequence thereof have important application value.

The present invention provides a primer-probe combination, a kit and an application for rapid detection of DON-producing Fusarium based on MIRA, which belongs to the field of biosafety technology. The present invention applies MIRA detection technology to DON-producing Fusarium detection for the first time, and compares the main DON-producing Fusarium with the Three Toxigenic gene clusters are screened for highly homologous sequences as specific amplification targets, and primer-probe combinations are designed for real-time fluorescence MIRA detection. The resulting primer and probe combination for detecting DON-producing Fusarium has strong specificity and high sensitivity. The method of detecting DON-producing Fusarium using a primer and probe combination for detecting DON-producing Fusarium has the advantages of simple operation, portable supporting equipment, short time consumption, high sensitivity, and automatic result interpretation. The amplification detection takes only about 20 minutes, which can achieve rapid on-site detection, timely discover the risk of DON-producing Fusarium contamination, intervene in advance, avoid risks or reverse losses.

The invention provides germacradienol biosynthase OsGMS and application of the germacradienol biosynthase OsGMS in prevention and control of pathogenic fungi and weeds, and belongs to the technical field of biosynthesis. The amino acid sequence of the rice germacradienol synthase OsGMS comprises an amino acid sequence as shown in SEQ ID NO: 1, or a functional homologous sequence with at least 75% sequence similarity with the amino acid sequence as shown in SEQ ID NO: 1, or one or more amino acids are added, deleted and replaced in the amino acid sequence as shown in SEQ ID NO: 1, or the amino acid sequence as shown in SEQ ID NO: 1 is a homologous sequence with at least 75% sequence similarity with the amino acid sequence as shown in SEQ ID NO: 2. And the protein formed by the amino acid sequence as shown in SEQ ID NO: 1 has an amino acid sequence with the same function. The encoding gene of the rice germacradienol synthase OsGMS is integrated into a carrier and introduced into a host cell, so that the encoding gene is expressed in the host cell, biosynthesis of germacradienol is realized, the germacradienol has the effect of inhibiting the growth of plant pathogenic fungi and weeds, and therefore, the germacradienol synthase OsGMS can be used for preparing novel biological pesticides capable of inhibiting the fungi and the weeds, and can be used for preparing biological pesticides for inhibiting the fungi and the weeds. And a new technical path is provided for developing environment-friendly agricultural preparations.

The invention discloses a method for detecting wheat differential transposon elements based on genome scanning, and belongs to the technical field of biology. The core of the invention focuses on a core technology system of crop genome TE difference detection and an application scene thereof, and comprises a detection method and a verification method. A homologous sequence distinguishing method based on an A / B / D subgenome SNP tag is protected, and the method comprises flanking region SNP matching rate calculation, a sliding window correction algorithm, a TE polymorphism extraction program and the like. The detection method provided by the invention effectively solves the problems of low efficiency of whole genome structure variation analysis, low detection specificity caused by polyploidy homologous sequence interference and high false positive of a single PCR primer pair verification system.

The invention relates to the technical field of gene engineering, in particular to a recombinant plasmid, a construction method and application of the recombinant plasmid to improvement of the synthesis capacity of nostoc flagelliforme pseudocladodin. The recombinant plasmid comprises a vector plasmid, and an sgRNA target sequence, an HR homologous sequence, a psbA promoter sequence and an ScyR regulator gene which are connected to the vector plasmid. The recombinant plasmid can over-express the key regulator gene at the MAAs gene cluster site of the nostoc flagelliforme, so that the pseudocladostin synthesis capability of the nostoc flagelliforme is remarkably improved. The recombinant plasmid is transformed into nostoc flagelliforme to obtain a nostoc flagelliforme mutant, and after the nostoc flagelliforme mutant is cultured for 15 days, the content of cladosporin is 2.4 times that of wild nostoc flagelliforme.

The present invention relates to a gene GmHB21 involved in soybean nodule vascular bundle development and its use. Specifically, the present invention relates to an isolated gene involved in soybean nodule vascular bundle development or regulating the number of soybean nodules, wherein the coding region sequence of the gene is shown in SEQ ID NO: 1 or a homologous sequence thereof. The present invention also provides a method for regulating the number of plant nodules, comprising introducing the gene described above, or a sequence that inhibits the expression of the gene described above, into target plant cells or tissues to obtain a transgenic plant, wherein the number of nodules in the transgenic plant is altered compared to the target plant, and the plant is a legume, preferably soybean. In addition, the present invention also provides the use of the gene described above, or the sequence that inhibits the expression of the gene described above, in cultivating legumes, such as soybeans, with altered nodule numbers.

Provided are a preparation method for a fusion polypeptide, and the use thereof. The fusion polypeptide is formed by directly linking SEQ ID NOs: 1-8 (referred to as O peptide) or a homologous sequence thereof, and SEQ ID NOs: 9-17 (referred to as G peptide) or a homologous sequence thereof, and a functional domain via a linker peptide (linker). The fusion polypeptide is formed by directly linking, in sequence from the N-terminus to the C-terminus, a G peptide or a homologous sequence thereof, an O peptide or a homologous sequence thereof, and a functional domain via a linker peptide (linker) (rigid or flexible); and the fusion polypeptide is formed by directly linking, in sequence from the N-terminus to the C-terminus, an O peptide or a homologous sequence thereof, a G peptide or a homologous sequence thereof, and a functional domain via a linker peptide (linker) (rigid or flexible). The fusion polypeptide has significant agonist activity against GPR158 and / or GPRC6A and / or GPR37, and also has significant antagonist activity against GPR1. The fusion polypeptide can be used in the preparation of drugs for treating alopecia, acne, dermatitis and other related diseases.

The invention discloses a leaf and branch compost cutinase link and application thereof, and belongs to the technical field of enzyme molecule construction. The leaf-branch compost cutinase kink is obtained by adjusting the connection sequence of fragments in leaf-branch compost cutinase, and comprises a fragment II, a connecting peptide fragment I, a fragment I, a connecting peptide fragment II and a fragment III which are connected in sequence from the N end to the C end; wherein the fragment I, the fragment II and the fragment III respectively correspond to 37th to 79th amino acids, 81st to 143rd amino acids and 150th to 293rd amino acids or homologous sequences thereof of the leaf branch compost cutinase. According to the method, protein topology engineering and an artificial intelligence assisted protein design technology are organically combined, knot topology transformation is performed on the leaf branch compost cutinase, sequence optimization is performed on a connecting peptide fragment of the knot, and the leaf branch compost cutinase knot with good biological activity is obtained; the expression quantity and the stability of the leaf and branch compost cutinase kink are improved, the problem of kinetic barriers existing in protein kink combination is solved, and the method has important application value.

This application relates to the field of plant genetic engineering, and particularly to the application of polynucleotides, proteins, and biological materials in regulating tuber development in plants, as well as related products and cultivation methods. The polynucleotide is the nucleotide sequence shown in SEQ ID NO:1; or its complementary sequence, degenerate sequence, or homologous sequence; or a polynucleotide that hybridizes with the nucleotide sequence shown in SEQ ID NO:1 under stringent conditions or its complementary sequence; or the cDNA sequence of any of the above sequences. This polynucleotide is an identity gene of the tuber, and can control whether tubers are formed, the formation time, and the regulation of tuber size, etc. by regulating protein function, expression level, etc.

The present invention discloses an L-aspartate aminotransferase mutant and its application. The L-aspartate aminotransferase mutant is obtained by subjecting the amino acid at position 104 of the amino acid sequence of L-aspartate aminotransferase to a single mutation. The L-aspartate aminotransferase mutant is used to synthesize L-phenylglycine derivatives. The L-aspartate aminotransferase mutant is derived from Escherichia coli. After homologous sequence analysis and molecular docking simulation, combined with site-directed mutagenesis verification, a single-point mutation is performed on the amino acid at position 104 to obtain a T104S mutant. The L-aspartate aminotransferase mutant of the present invention can efficiently catalyze the production of L-phenylglycine derivatives. Compared with the wild type, the specific activity of the T104S mutant towards o-chlorophenoxyacetic acid is 3.2 times that of the wild type.

The invention relates to the technical field of antibody detection, discloses an antibody sequence detection method, a medium and equipment, and can improve the structural detection precision of a CDR-H3 ring in an antibody. The method comprises the following steps: acquiring a first antibody sequence, wherein the first antibody sequence comprises a heavy chain and a light chain which are paired; a first feature, a second feature and a third feature of the first antibody sequence are obtained, the first feature is used for indicating evolutionary constraint information between the first antibody sequence and the homologous sequence, and the second feature is used for indicating a mutual relation between amino acid pairs in the first antibody sequence; the third feature is used for indicating a dependency relationship between amino acids in the first antibody sequence; performing fusion processing on the first feature and the third feature to obtain a fusion feature of the first antibody sequence; and detecting to obtain a three-dimensional structure of the first antibody sequence according to the second feature and the fusion feature.

Sequence data is received that specifies at least one sequence of interest. Thereafter, homologous sequence are collected based on the sequence data and are represented in a multiple sequence alignment using a novel search approach. Next, an epistatic model is computed by a first machine learning model that represents a revolutionary landscape of the multiple sequence alignment. Later, a second machine learning model is used to iteratively generate statistical inferences based upon the epistatic model, to result in a candidate pool of sequences comprising variants of the sequence of interest. Data can then be provided which characterizes the candidate pool of sequences.

The present application relates to a multiplex PCR primer probe combination and kit for human red blood cell RHD gene typing detection, and belongs to the field of biomedical clinical molecular detection. The multiplex PCR primer probe combination for human red blood cell RHD gene typing detection comprises 19 pairs of specific primers and corresponding probes distributed in 8 reaction wells. The present application also provides a kit containing the multiplex PCR primer probe combination for human red blood cell RHD gene typing detection. The RHD gene variant site specific primers and probes designed by ARMS combined with homologous sequence specific base method are more specific and accurate; all high frequency RHD gene variants of Chinese population can be detected by one experiment, including DEL type (RHD*DEL1, c.1227G>A), weak D15 type (RHD*15), partial RHD*DVI.3 type (RHD*DVI.3), RHD deletion type D negative (RHD*01N.01) and other common D negative phenotypes of Chinese population, RHD*D-CE(2-9)-D (RHD*01N.03) and RHD*01N.16 (c.711delC), and normal RHD positive types (RHD*01) can also be detected.