39 results about "Introgression" patented technology

The invention discloses a method for creating a high-frequency introgression Chinese cabbage-cabbage introgression line, and relates to the technical field of introgression breeding. According to the method, tetraploid Chinese cabbage and diploid cabbage are subjected to interspecific hybridization to obtain an allotriploid hybrid (AAC), then the AAC is subjected to hybridization and selfing with diploid Chinese cabbage, and a Chinese cabbage-cabbage introgression line with a high-frequency introgression exogenous species genome sequence is created in combination with free microspore culture. According to the invention, the heterotriploid hybrid mediated genetic system is utilized to realize interspecific high introgression, and a new method is provided for breaking the bottleneck of low exogenous species introgression frequency.

The invention relates to the technical field of biology, and particularly discloses a method for regulating expression of an SH3.1 gene to reduce rice seed shattering and application of the method. The method for reducing the seed shattering of the rice comprises the following steps: inhibiting the expression of an SH3.1 gene in receptor rice to obtain target rice of which the seed shattering is lower than that of the receptor rice. The inventor adopts a CRISPR / Cas9 technology to edit an SH3.1 gene at a fixed point, and knocks out the SH3.1 gene of an introgression line GIL291 of an African cultivated rice IRGC104038 segment by causing frameshift mutation, so that a new rice germplasm with obviously reduced seed shattering is obtained. High seed shattering is an important factor restricting yield increase of African cultivated rice, the rice seed shattering is reduced, high-yield germplasm resources of rice are innovated, and the method has important significance on new variety cultivation, environmental sanitation and grain safety. The method has great application and popularization values.

The present invention relates to a method for identifying Aegilops tauschii-derived germplasm fragments by using an exon capture sequencing chip of the D genome of wheat crops. The method comprises the following steps: performing capture sequencing on the derivative introgression line material and the parents of the derivative introgression line material by using an exon capture sequencing chip of the pan-gene exons of the wheat D genome; aligning the capture sequencing data of the derivative introgression line material and the parents of the derivative introgression line material to the wheat reference genome to obtain a derivative introgression line material differential site file and a parental differential site file; filtering the parental differential site file against the introgression line material differential site file and retaining the intersection of the two differential site files; using the intersection file and the parental differential site file to calculate the exogenous introgression ratio of the derivative introgression line material within a window; using the introgression ratio file to draw a dot plot, and defining the region with continuous high introgression ratio as the exogenous fragment. The present invention can be accurately used for identifying exogenous fragments of introgression lines or synthetic materials derived from Aegilops tauschii.

The application belongs to the technical field of genetic engineering, and particularly relates to a ten-ploid long-spike Aegilops tauschii introgression gene ThpFBX1 and application thereof in regulating the heading stage of wheat. The amino acid sequence of the ten-ploid long-spike Aegilops tauschii introgression gene ThpFBX1 is shown as SEQ ID No. 1, and the nucleotide sequence is shown as SEQ ID No. 2. The ten-ploid long-spike Aegilops tauschii introgression gene ThpFBX1 is introduced into a recipient plant to obtain a transgenic plant overexpressing the ThpFBX1 gene, and experiments show that the overexpressing wheat exhibits early heading, and the ThpFBX1 gene can significantly advance the heading stage of the wheat.

The application provides an analysis method for complex evolutionary history based on deep learning, comprising: according to a preset species evolutionary history simulation sequence data, respectively as a model training set and a test set; determining the topological structure of the training set data, and labeling the training data in different topological structure proportions; constructing a convolutional neural network, training and testing the convolutional neural network with the data set, so that the error between the data prediction value and the label value is minimized; based on the trained convolutional neural network, analyzing real genomic sequence data, and combining other population genetics analysis methods, determining the evolutionary relationship and introgression site between different biological groups. The application uses comparative genome or population genome data, infers the topological structure between sequences through a deep learning algorithm, further evaluates the evolutionary relationship at the genome level, and identifies local introgression signals through the difference of the topological structure between different regions.

The present invention discloses a method for efficiently identifying ancient ancestral introgressed genes in poplar trees. The method comprises the following steps: obtaining a SNP dataset for a poplar population, obtaining the population structure of the poplar population, preliminarily locating the introgressed regions of the poplar population, locating candidate ancestral introgressed regions of the poplar population, obtaining the ancient ancestral introgressed regions of the poplar population, and obtaining ancient ancestral introgressed genes of the poplar population. The method disclosed in the present invention can effectively reduce false positives, reduce computational complexity, and increase identification speed. It can also obtain ancestral gene flow in multiple poplar populations, accurately identify ancient ancestral introgressed genes in poplar trees, and has a wide range of applicability.

The present invention discloses a method for developing and applying specific single-locus IP markers in the radish genome. This method utilizes bioinformatics techniques to develop specific single-locus IP markers in radish based on the genomic information of radish and Brassica crops; and these markers are actually applied to the identification of exogenous introgressed fragments in the progeny of radish-Brassica interspecific hybridization. The present invention realizes the rapid identification of excellent gene fragments in the progeny of distant hybridization between radish as the donor and Brassica crops, effectively assists the precise introgression of excellent gene fragments, accurately tracks the size of the gene fragments involved at the same time, breaks the linkage drag of bad traits, and realizes the efficient creation and utilization of germplasm resources. At the same time, it provides important data support for the identification of chromosomal fragments of genes related to improved traits in hybrid progeny.

The invention provides fertility restorer Brassica napus plants, plant material and seeds, characterized in that these products harbor a specific introgression fragment of the Ogura fertility restorer at the end of chromosome N 10. Tools are also provided which allow detection of the fertility restorer.

This invention discloses an InDel marker, Pmct3, for cold tolerance during rice budding, along with its identification method and applications, belonging to the field of molecular marker technology. This invention develops an InDel molecular marker from the insertion / deletion sequence between the Dongxiang wild rice introgression line D2 (background of the cold-tolerant rice 9311) and the cold-sensitive rice 9311 at the Chr1:28249918 and Chr1:28249919 sites in the O. sativa reference genome. Primers are then developed based on this molecular marker for identifying cold tolerance during rice budding. The detection cycle of this invention is only 2-3 hours, does not rely on subjective experience in traditional detection methods, and can obtain accurate and objective detection and identification results in a short time. It is low-cost, high-throughput, and highly specific, making it suitable for rice breeding and production practices.

The invention discloses an InDel marker Pmct1 for the cold-tolerant character of rice in the bud stage as well as an identification method and application of the InDel marker Pmct1, and belongs to the technical field of molecular markers. The InDel molecular marker is developed from insertion and deletion sequences of a Dongxiang wild rice introgression line D2 under the background of a bud-stage strong cold-resistant material 9311 and a bud-stage cold-sensitive material 9311 at an O.sativa reference genome Chr1: 32106831.. 32106871 site, and a primer is developed according to the molecular marker, so that the InDel molecular marker is used for identifying the bud-stage cold-resistant character of rice. The detection period of the method only needs 2-3 h, the method does not depend on subjective experience of people in traditional detection, accurate and objective detection and identification results can be obtained in a short time, and the method is low in cost, high in flux, high in specificity and suitable for rice breeding and production practice.

The present invention discloses a molecular marker for screening drought-resistant poplars based on the interspecific introgression theory, as well as a method and application thereof. The molecular marker is located at the 1022nd base upstream of the PtoTCP19 gene and has a T / C polymorphism. Individuals with the CC genotype of the molecular marker have the strongest drought resistance. The molecular marker provided by the present invention, obtained based on the interspecific introgression theory, has high accuracy and can accurately evaluate the drought resistance of poplars at the molecular level. It can accurately and efficiently screen drought-resistant tree species during the seedling stage, shortening the breeding cycle, and is of great significance for enhancing plant stress resistance and mitigating the damage caused by drought adversity to forestry yield and quality.

The present invention relates to a Capsicum annuum plant comprising an introgression fragment on chromosome 8 comprising Quantitative Trait Locus QTL8, wherein said QTL8 confers an improved resistance to Frankliniella occidentalis. The present invention further relates to a seed produced by the plant according to the present invention, a seed from which a plant according to present invention can be grown, a fruit produced by a plant according to the present invention and a part of a plant according to the present invention. The present invention further relates to a method of identifying and / or selecting a plant or plant part according to the present invention. The present invention further relates to a method for producing a Capsicum annuum plant having the improved resistance to Frankliniella occidentalis according to the present invention. The present invention further relates to a method for improving the resistance of a Capsicum annuum plant to Frankliniella occidentalis, the use of QTL8 according to the present invention for improving the resistance of a Capsicum annuum plant to Frankliniella occidentalis. The present invention further relates to genetic markers specific for QTL8 according to the present invention and the use thereof for selecting a Capsicum annuum plant having an improved resistance to Frankliniella occidentalis.

The invention discloses a method for efficiently identifying an ancestral ancient introgression gene of a poplar. The method comprises the following steps: obtaining an SNP data set of a poplar population, obtaining a population structure of the poplar population, preliminarily positioning an introgression region of the poplar population, positioning a candidate ancestor introgression region of the poplar population, obtaining an ancestor ancient introgression region of the poplar population, and obtaining an ancestor ancient introgression gene of the poplar population. The method disclosed by the invention can effectively reduce false positive, reduce the calculation amount and improve the identification speed, can obtain the progenitor gene flow of multiple populations of the poplar, can accurately identify the progenitor paleo-introgression gene of the poplar, and is wide in application range.

The invention discloses a DNA bar code system and method for identifying glycyrrhiza medicinal plants and hybrid complexes thereof, and belongs to the technical field of molecular biology and traditional Chinese medicine identification. The system is formed by combining three bar codes of a nuclear gene segment ITS, a chloroplast gene segment ndhA and a chloroplast gene spacer trnH-psbA. Wherein ndhA is a core bar code which is screened and verified for the first time, and the specific variation site of the ndhA can be used for accurately distinguishing three kinds of medicinal liquorice, namely, Glycyrrhiza uralensis Fischhex DC., Glycyrrhiza glabra. And Glycyrrhiza inflata Batalin. The system fully utilizes the characteristics of chloroplast paternal heredity of glycyrrhiza, not only can identify homozygous species, but also can efficiently analyze complex hybridization and introgression types, and traces the source of a male parent. Compared with a traditional multi-bar code combination, the method has the advantages of being high in identification success rate (up to 98.08%), easy and convenient to operate and low in cost, and is suitable for liquorice germplasm resource identification, medicinal material market rapid screening and improved variety breeding.

The application discloses an application of a leucine-rich repeat receptor-like kinase gene of Gossypium anomalum, which is composed of 3885 bases, wherein the first base from the 5' end is a transcription start site, and the 3885th base is a transcription termination site. Expression of the gene can cause a lethal phenotype of a single segment introgression line CSSL11-9 of the Gossypium anomalum. The lethal phenotype is inhibited by silencing the gene expression in the single segment introgression line CSSL11-9 of the Gossypium anomalum, and the single segment introgression line CSSL11-9 can survive normally. In addition, the gene can also endow cotton with resistance to verticillium wilt and fusarium wilt, and the resistance of the CSSL11-9 plant with the silenced gene to the cotton verticillium wilt and fusarium wilt is significantly reduced, thereby providing an important gene resource for cotton breeding against the verticillium wilt and the fusarium wilt.

A method identifies and selects plants resistant to a fungal disease comprises (a) extraction of nucleic acid from a plant; (b) analysis of extracted nucleic acid for the presence of markers associated with increased fungal resistance within a single chromosome interval; and (c) selection of the plants that have these markers. Furthermore, a method sufficient for introgression into plants of fungal disease resistance alleles comprises (a) crossing parents of plants identified by the first embodiment method with other parents that do not have this resistance; (b) select progenies possessing markers associated with increased resistance to fungal disease using the method as defined in the first achievement; and (c) backcross in one or more cycles the selected progenies with the recurrent genitor to develop new progenies.

The invention discloses a molecular marker for screening drought-resistant poplars based on an interspecific introgression theory and a method and application thereof, the molecular marker is located at the 1022nd basic group of the upstream of a PtoTCP19 gene and has T / C polymorphism, the genotype of the molecular marker is a CC individual, and the drought resistance of the molecular marker is the strongest. The molecular marker provided by the invention is obtained based on an interspecific introgression theory, is high in accuracy, can be used for accurately evaluating the drought resistance of the poplar at a molecular level, accurately and efficiently screening drought-resistant tree species at a seedling stage and shortening a breeding period, and is of great significance in enhancing plant stress resistance and relieving damage of drought stress to forestry yield and quality.

This application discloses the application of a KASP molecular marker for round-fruited melons in identifying melon fruit type, belonging to the field of genetic engineering technology. The KASP molecular marker is located at 21758042 bp on chromosome 9 of the melon, with an SNP locus of G / T, where the G genotype corresponds to round fruit type and the T genotype corresponds to long fruit type. This application utilizes a hybrid population constructed from the long-fruited melon 'Yangjiaomi' and the round-fruited melon 'VED', combined with FSI9.1-6 molecular marker-assisted selection breeding, enabling high-throughput rapid identification of melon round fruit type directly at the seedling stage. This molecular marker can be used to construct introgression lines for melon fruit type, achieving directional improvement from long-fruited to round-fruited melons, effectively shortening the breeding cycle and improving breeding efficiency, and has significant application value for accelerating the molecular breeding process of melons and cultivating high-quality varieties.

Celery plant (Apium graveolens L. dulce) with QTL on chromosome 8 between SEQ ID No. 1 and SEQ ID No. 2, which when homozygously present is responsible for the presence of two internodes on average per leaf at the harvesting stage (Reduced foliage or “Rf”-type ”). The QTL can be genetically linked to at least one SNP present in SEQ ID No. 1 and / or SEQ ID No. 2. Celery seed deposited as NCIMB 44381 or NCIMB 41513 has the QTL. Celery plant having the QTL or genetic determinant having the QTL leads to the Rf-type (compared to celery not carrying the genetic determinant or QTL). The determinant or QTL is obtainable by introgression from a plant from NCIMB 41513 or 44381. Crossing celery from NCIMB 44381 or NCIMB 41513 with wildtype celery results in segregated F2 progeny population wherein Rf-type is observed in a monogenic recessive fashion. Methods for producing or growing the celery plant, and seeds, propagation material, progeny, and food products of, from the celery plant also disclosed.

The present invention discloses the mapping of a pair of dominant complementary clubroot-resistant genes in Chinese cabbage and closely linked SSR molecular markers and their applications, belonging to the fields of crop breeding and biomolecular genetics. In the present invention, the disease-resistant material 'CR-20' is crossed with the disease-susceptible material 'BJN3-1', and the disease-resistant genes contained in the disease-resistant material are preliminarily mapped by constructing an F2 generation genetic segregation population. By adopting the method of genetic map mapping combined with BSA sequencing, the disease-resistant locus is finally mapped between the markers SZC-7 and LSN-3-115 on A01, and between the markers ACMP00373 and SZC-37 on A08. The two disease-resistant loci show a dominant complementary genetic relationship. The present invention has found molecular markers closely linked to the disease-resistant genes contained in 'CR-20', completed the preliminary mapping of this locus, provided technical support for the future transfer of clubroot resistance in Brassica crops using the 'CR-20' material, and provided more options for the research on clubroot resistance breeding of Chinese cabbage and other Brassica crops in China.

The present invention belongs to the field of crop molecular breeding and functional research, and specifically relates to a Triticeae D-genome pan-gene exon capture sequencing chip and its design method. The present invention integrates the Aegilops tauschii genome and the wheat D-subgenome, extracts gene coding region sequences by an iterative method, and uses Kmer classification and counting for grouped amplification to obtain Aegilops tauschii pan-genome capture probes, which are the Triticeae D-genome pan-gene exon capture sequencing chips. The present invention adopts the design of pan-genome probes, which can be better applied to the detection of different D-subgenome-derived materials and can cover the genetic variations of different Aegilops tauschii. The exon capture sequencing chip of the present invention can not only achieve high coverage of the exon regions of the whole genome derived from Aegilops tauschii, but also accurately identify exogenous fragments in introgression lines or synthetic materials derived from Aegilops tauschii, and greatly reduces the sequencing and analysis costs.

The invention discloses application of a muskmelon round fruit type KASP molecular marker in identification of sweet melon fruit types, and belongs to the technical field of gene engineering. The KASP molecular marker is located at the 21758042bp position of a muskmelon chromosome 9, the SNP site is G / T, the G genotype corresponds to a round fruit type, and the T genotype corresponds to a long fruit type. According to the application, the hybrid population is constructed by utilizing the long-fruit muskmelon'horn honey 'and the round-fruit muskmelon'VED', and the FSI9.1-6 molecular marker is combined for assisted selective breeding, so that high-throughput rapid identification of the round-fruit muskmelon can be directly realized in the seedling stage. The molecular marker can be used for constructing a sweet melon and fruit type introgression line, realizes directional improvement from long-fruit muskmelons to round-fruit muskmelons, effectively shortens the breeding period, improves the breeding efficiency, and has important application value for accelerating the molecular breeding process of muskmelons and breeding high-quality varieties.

The invention discloses application of a rice gene OsZP2 in regulation and control of rice disease resistance, the gene is used for positively regulating and controlling rice sheath blight resistance and rice blast resistance, specifically, the OsZP2 gene from japonica rice variety Nipponbare (NIP) is introduced into indica rice variety by utilizing a transgenosis method, and rhizoctonia solani and rice blast are inoculated respectively, so that the rice disease resistance of rice is regulated and controlled. The disease spot area or length of the transgenic (OsZP2) plant is reduced, and the disease resistance is enhanced; the OsZP2 gene of NIP is introduced into an indica rice variety by molecular marker-assisted selection to obtain a single-fragment introgression line containing the OsZP2 gene, rhizoctonia solani and magnaporthe oryzae are inoculated respectively, the area or length of disease spots of a plant containing the introgression line containing the OsZP2 gene is reduced, and the disease resistance is enhanced.

The present invention relates to cultivated cucumber plants comprising a yield QTL on chromosome 3 of their genome, and to methods for generating such plants, and their use.

The invention discloses a salt tolerance related SSR sequence derived from cotton diploid wild species abnormal cotton and application thereof, the SSR sequence belongs to a specific DNA fragment of an abnormal cotton introgression line CSSL44, and is located on a No.6 chromosome of an abnormal cotton chromosome group. The specific DNA fragment provided by the invention has an important application value in salt-tolerant cotton breeding, meanwhile, the 9 pairs of SSR molecular markers developed by the invention provide a molecular basis for cultivating salt-tolerant cotton varieties which can be applied to production, and by utilizing the molecular markers provided by the invention, the salt-tolerant character of a cotton plant can be quickly identified, and the application prospect is wide. And a technical foundation is laid for research on a cotton salt tolerance improving mechanism.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to drug resistance of eimeria maduramicin and application of the SNP molecular marker. The method comprises the following steps: hybridizing a chicken eimeria tenella maduramicin drug-resistant strain with a sensitive strain, and backcrossing the hybridized strain with the sensitive strain to construct a maduramicin drug-resistant introgression line strain. By means of a genome re-sequencing technology and a gene editing technology, polymorphic mutation exists in the 1255th nucleotide of the ETH20402100 gene, C corresponds to a maduramicin sensitive phenotype, and T corresponds to a maduramicin drug-resistant phenotype. Based on a PCR identification result of a field sample, the SNP molecular marker can accurately detect the drug resistance of maduramicin, and the result is consistent with a cage feeding experiment. The invention provides a primer for detection and a method for screening drug-resistant strains by combining a PCR (Polymerase Chain Reaction) technology, and the method is simple to operate, short in time consumption and low in cost, and can be widely applied to clinical detection of the drug resistance of chicken coccidiosis maduramicin and drug use guidance.

The application relates to the technical field of molecular biology, and discloses an InDel molecular marker linked with a wheat-ice grass introgression line powdery mildew resistance site PmYZW-6A-1 and application thereof. The InDel molecular marker linked with the wheat-ice grass introgression line powdery mildew resistance site PmYZW-6A-1 is located at the 231th-257th nucleotide sequence in SEQ ID NO. 1, and the resistance site PmYZW-6A-1 is located at 442,022,338 bp of the 6A chromosome of the Pujing 3504 reference genome. Through the technical scheme, the problems that genetic diversity cannot be efficiently utilized by traditional wheat breeding methods and a wide-spectrum and long-lasting disease-resistant variety has low cultivation efficiency are solved.

The invention discloses a KASP primer group of an aegilops tauschii 4D chromosome, a kit and application, and belongs to the technical field of molecular biology. The kit comprises six KASP primer groups, each primer group comprises two forward primers and one reverse primer, and the specific sequences of the primers are as shown in SEQ ID NO.1-18. After the aegilops tauschii and common wheat are subjected to whole genome comparison, the specific SNP of the aegilops tauschii is obtained through screening, and then the corresponding specific marker is developed, so that the marker developed by the invention can be used for tracking and detecting 4D chromosome introgression fragments or genes which are rich in genetic variation and originated from Straangula type aegilops tauschii AT23; the method has important value for genetic breeding improvement of common wheat.