5 results about "Introgression" patented technology

The application belongs to the technical field of genetic engineering, and particularly relates to a ten-ploid long-spike Aegilops tauschii introgression gene ThpFBX1 and application thereof in regulating the heading stage of wheat. The amino acid sequence of the ten-ploid long-spike Aegilops tauschii introgression gene ThpFBX1 is shown as SEQ ID No. 1, and the nucleotide sequence is shown as SEQ ID No. 2. The ten-ploid long-spike Aegilops tauschii introgression gene ThpFBX1 is introduced into a recipient plant to obtain a transgenic plant overexpressing the ThpFBX1 gene, and experiments show that the overexpressing wheat exhibits early heading, and the ThpFBX1 gene can significantly advance the heading stage of the wheat.

The application provides an analysis method for complex evolutionary history based on deep learning, comprising: according to a preset species evolutionary history simulation sequence data, respectively as a model training set and a test set; determining the topological structure of the training set data, and labeling the training data in different topological structure proportions; constructing a convolutional neural network, training and testing the convolutional neural network with the data set, so that the error between the data prediction value and the label value is minimized; based on the trained convolutional neural network, analyzing real genomic sequence data, and combining other population genetics analysis methods, determining the evolutionary relationship and introgression site between different biological groups. The application uses comparative genome or population genome data, infers the topological structure between sequences through a deep learning algorithm, further evaluates the evolutionary relationship at the genome level, and identifies local introgression signals through the difference of the topological structure between different regions.

The invention provides fertility restorer Brassica napus plants, plant material and seeds, characterized in that these products harbor a specific introgression fragment of the Ogura fertility restorer at the end of chromosome N 10. Tools are also provided which allow detection of the fertility restorer.

A method identifies and selects plants resistant to a fungal disease comprises (a) extraction of nucleic acid from a plant; (b) analysis of extracted nucleic acid for the presence of markers associated with increased fungal resistance within a single chromosome interval; and (c) selection of the plants that have these markers. Furthermore, a method sufficient for introgression into plants of fungal disease resistance alleles comprises (a) crossing parents of plants identified by the first embodiment method with other parents that do not have this resistance; (b) select progenies possessing markers associated with increased resistance to fungal disease using the method as defined in the first achievement; and (c) backcross in one or more cycles the selected progenies with the recurrent genitor to develop new progenies.

This application discloses the application of a KASP molecular marker for round-fruited melons in identifying melon fruit type, belonging to the field of genetic engineering technology. The KASP molecular marker is located at 21758042 bp on chromosome 9 of the melon, with an SNP locus of G / T, where the G genotype corresponds to round fruit type and the T genotype corresponds to long fruit type. This application utilizes a hybrid population constructed from the long-fruited melon 'Yangjiaomi' and the round-fruited melon 'VED', combined with FSI9.1-6 molecular marker-assisted selection breeding, enabling high-throughput rapid identification of melon round fruit type directly at the seedling stage. This molecular marker can be used to construct introgression lines for melon fruit type, achieving directional improvement from long-fruited to round-fruited melons, effectively shortening the breeding cycle and improving breeding efficiency, and has significant application value for accelerating the molecular breeding process of melons and cultivating high-quality varieties.