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259 results about "Introgression" patented technology

Introgression, also known as introgressive hybridization, in genetics is the movement of a gene (gene flow) from one species into the gene pool of another by the repeated backcrossing of an interspecific hybrid with one of its parent species. Purposeful introgression is a long-term process; it may take many hybrid generations before the backcrossing occurs.

Molecular marker for rice brown planthopper-resistance QBph3 and QBph4 genes

The invention belongs to the technical field of preparation of a rice molecular marker, and in particular relates to a molecular marker for two rice brown planthopper-resistance major genes QBph3 and QBph4 which are simultaneously derived from a pest-resistance introgression line IR02W101. The molecular marker comprises the following steps: carrying out crossing, self-crossing and backcrossing on Zhenshan97, which is an herbivore-susceptible variety, and IR02W101 so as to obtain genotypes of various BC1F2 single plants; carrying out genetic linkage analysis in accordance with brown planthopper-resistance grades of various F2: 3 lines during seedling stage; precisely locating a resistance gene QBph3 of the IR02W101 between long-arm markers t6 and f3 of the 3rd chromosome so as to obtain a co-segregative marker c3-14 as well as closely linked markers q1 and m3; and precisely locating QBph4 between short-arm markers p17 and xc4-27 of the 4th chromosome so as to obtain closely linked markers p6, p9, c4-5, xc4-7, HJ16, J417 and IN156. The molecular marker disclosed by the invention can be used for effectively detecting whether the pest-resistance introgression line IR02W101 and derived varieties thereof contain the major gene site or not.
Owner:HUAZHONG AGRI UNIV
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