16 results about "Introgression" patented technology

The application belongs to the technical field of genetic engineering, and particularly relates to a ten-ploid long-spike Aegilops tauschii introgression gene ThpFBX1 and application thereof in regulating the heading stage of wheat. The amino acid sequence of the ten-ploid long-spike Aegilops tauschii introgression gene ThpFBX1 is shown as SEQ ID No. 1, and the nucleotide sequence is shown as SEQ ID No. 2. The ten-ploid long-spike Aegilops tauschii introgression gene ThpFBX1 is introduced into a recipient plant to obtain a transgenic plant overexpressing the ThpFBX1 gene, and experiments show that the overexpressing wheat exhibits early heading, and the ThpFBX1 gene can significantly advance the heading stage of the wheat.

The application provides an analysis method for complex evolutionary history based on deep learning, comprising: according to a preset species evolutionary history simulation sequence data, respectively as a model training set and a test set; determining the topological structure of the training set data, and labeling the training data in different topological structure proportions; constructing a convolutional neural network, training and testing the convolutional neural network with the data set, so that the error between the data prediction value and the label value is minimized; based on the trained convolutional neural network, analyzing real genomic sequence data, and combining other population genetics analysis methods, determining the evolutionary relationship and introgression site between different biological groups. The application uses comparative genome or population genome data, infers the topological structure between sequences through a deep learning algorithm, further evaluates the evolutionary relationship at the genome level, and identifies local introgression signals through the difference of the topological structure between different regions.

The invention provides fertility restorer Brassica napus plants, plant material and seeds, characterized in that these products harbor a specific introgression fragment of the Ogura fertility restorer at the end of chromosome N 10. Tools are also provided which allow detection of the fertility restorer.

The present invention relates to a Capsicum annuum plant comprising an introgression fragment on chromosome 8 comprising Quantitative Trait Locus QTL8, wherein said QTL8 confers an improved resistance to Frankliniella occidentalis. The present invention further relates to a seed produced by the plant according to the present invention, a seed from which a plant according to present invention can be grown, a fruit produced by a plant according to the present invention and a part of a plant according to the present invention. The present invention further relates to a method of identifying and / or selecting a plant or plant part according to the present invention. The present invention further relates to a method for producing a Capsicum annuum plant having the improved resistance to Frankliniella occidentalis according to the present invention. The present invention further relates to a method for improving the resistance of a Capsicum annuum plant to Frankliniella occidentalis, the use of QTL8 according to the present invention for improving the resistance of a Capsicum annuum plant to Frankliniella occidentalis. The present invention further relates to genetic markers specific for QTL8 according to the present invention and the use thereof for selecting a Capsicum annuum plant having an improved resistance to Frankliniella occidentalis.

The invention discloses a DNA bar code system and method for identifying glycyrrhiza medicinal plants and hybrid complexes thereof, and belongs to the technical field of molecular biology and traditional Chinese medicine identification. The system is formed by combining three bar codes of a nuclear gene segment ITS, a chloroplast gene segment ndhA and a chloroplast gene spacer trnH-psbA. Wherein ndhA is a core bar code which is screened and verified for the first time, and the specific variation site of the ndhA can be used for accurately distinguishing three kinds of medicinal liquorice, namely, Glycyrrhiza uralensis Fischhex DC., Glycyrrhiza glabra. And Glycyrrhiza inflata Batalin. The system fully utilizes the characteristics of chloroplast paternal heredity of glycyrrhiza, not only can identify homozygous species, but also can efficiently analyze complex hybridization and introgression types, and traces the source of a male parent. Compared with a traditional multi-bar code combination, the method has the advantages of being high in identification success rate (up to 98.08%), easy and convenient to operate and low in cost, and is suitable for liquorice germplasm resource identification, medicinal material market rapid screening and improved variety breeding.

The application discloses an application of a leucine-rich repeat receptor-like kinase gene of Gossypium anomalum, which is composed of 3885 bases, wherein the first base from the 5' end is a transcription start site, and the 3885th base is a transcription termination site. Expression of the gene can cause a lethal phenotype of a single segment introgression line CSSL11-9 of the Gossypium anomalum. The lethal phenotype is inhibited by silencing the gene expression in the single segment introgression line CSSL11-9 of the Gossypium anomalum, and the single segment introgression line CSSL11-9 can survive normally. In addition, the gene can also endow cotton with resistance to verticillium wilt and fusarium wilt, and the resistance of the CSSL11-9 plant with the silenced gene to the cotton verticillium wilt and fusarium wilt is significantly reduced, thereby providing an important gene resource for cotton breeding against the verticillium wilt and the fusarium wilt.

A method identifies and selects plants resistant to a fungal disease comprises (a) extraction of nucleic acid from a plant; (b) analysis of extracted nucleic acid for the presence of markers associated with increased fungal resistance within a single chromosome interval; and (c) selection of the plants that have these markers. Furthermore, a method sufficient for introgression into plants of fungal disease resistance alleles comprises (a) crossing parents of plants identified by the first embodiment method with other parents that do not have this resistance; (b) select progenies possessing markers associated with increased resistance to fungal disease using the method as defined in the first achievement; and (c) backcross in one or more cycles the selected progenies with the recurrent genitor to develop new progenies.

This application discloses the application of a KASP molecular marker for round-fruited melons in identifying melon fruit type, belonging to the field of genetic engineering technology. The KASP molecular marker is located at 21758042 bp on chromosome 9 of the melon, with an SNP locus of G / T, where the G genotype corresponds to round fruit type and the T genotype corresponds to long fruit type. This application utilizes a hybrid population constructed from the long-fruited melon 'Yangjiaomi' and the round-fruited melon 'VED', combined with FSI9.1-6 molecular marker-assisted selection breeding, enabling high-throughput rapid identification of melon round fruit type directly at the seedling stage. This molecular marker can be used to construct introgression lines for melon fruit type, achieving directional improvement from long-fruited to round-fruited melons, effectively shortening the breeding cycle and improving breeding efficiency, and has significant application value for accelerating the molecular breeding process of melons and cultivating high-quality varieties.

The invention discloses application of a muskmelon round fruit type KASP molecular marker in identification of sweet melon fruit types, and belongs to the technical field of gene engineering. The KASP molecular marker is located at the 21758042bp position of a muskmelon chromosome 9, the SNP site is G / T, the G genotype corresponds to a round fruit type, and the T genotype corresponds to a long fruit type. According to the application, the hybrid population is constructed by utilizing the long-fruit muskmelon'horn honey 'and the round-fruit muskmelon'VED', and the FSI9.1-6 molecular marker is combined for assisted selective breeding, so that high-throughput rapid identification of the round-fruit muskmelon can be directly realized in the seedling stage. The molecular marker can be used for constructing a sweet melon and fruit type introgression line, realizes directional improvement from long-fruit muskmelons to round-fruit muskmelons, effectively shortens the breeding period, improves the breeding efficiency, and has important application value for accelerating the molecular breeding process of muskmelons and breeding high-quality varieties.

The invention discloses a salt tolerance related SSR sequence derived from cotton diploid wild species abnormal cotton and application thereof, the SSR sequence belongs to a specific DNA fragment of an abnormal cotton introgression line CSSL44, and is located on a No.6 chromosome of an abnormal cotton chromosome group. The specific DNA fragment provided by the invention has an important application value in salt-tolerant cotton breeding, meanwhile, the 9 pairs of SSR molecular markers developed by the invention provide a molecular basis for cultivating salt-tolerant cotton varieties which can be applied to production, and by utilizing the molecular markers provided by the invention, the salt-tolerant character of a cotton plant can be quickly identified, and the application prospect is wide. And a technical foundation is laid for research on a cotton salt tolerance improving mechanism.

The application relates to the technical field of molecular biology, and discloses an InDel molecular marker linked with a wheat-ice grass introgression line powdery mildew resistance site PmYZW-6A-1 and application thereof. The InDel molecular marker linked with the wheat-ice grass introgression line powdery mildew resistance site PmYZW-6A-1 is located at the 231th-257th nucleotide sequence in SEQ ID NO. 1, and the resistance site PmYZW-6A-1 is located at 442,022,338 bp of the 6A chromosome of the Pujing 3504 reference genome. Through the technical scheme, the problems that genetic diversity cannot be efficiently utilized by traditional wheat breeding methods and a wide-spectrum and long-lasting disease-resistant variety has low cultivation efficiency are solved.

The invention discloses a KASP primer group of an aegilops tauschii 4D chromosome, a kit and application, and belongs to the technical field of molecular biology. The kit comprises six KASP primer groups, each primer group comprises two forward primers and one reverse primer, and the specific sequences of the primers are as shown in SEQ ID NO.1-18. After the aegilops tauschii and common wheat are subjected to whole genome comparison, the specific SNP of the aegilops tauschii is obtained through screening, and then the corresponding specific marker is developed, so that the marker developed by the invention can be used for tracking and detecting 4D chromosome introgression fragments or genes which are rich in genetic variation and originated from Straangula type aegilops tauschii AT23; the method has important value for genetic breeding improvement of common wheat.

The invention belongs to the technical field of wheat breeding, and relates to a molecular marker for detecting haplotype of an aegilops tauschii sourced stripe rust resistant gene Yr28 and application of the molecular marker. According to the invention, a dCAPS marker is designed according to the base difference between the AetAY614Dv1G0004900.1 gene of aegilops tauschii AY61 and the 18th site of a CDS region in the Yr28 allele of aegilops tauschii PI511383, and the marker can effectively identify different haplotypes of the Yr28 gene in the aegilops tauschii germplasm resources. In addition, a KASP marker is developed on the basis of the base difference between the new haplotype of the stripe rust resistance gene Yr28 in the aegilops tauschii AY61 and the TraesAK58CH4D01G004400.1 gene of common wheat AK58, the introgression line genotype created by the common wheat and the aegilops tauschii AY61 can be efficiently identified, and identification and screening of disease-resistant single plants in hybridization or backcross derivative populations are achieved.

The invention discloses a primer group for detecting a KASP molecular marker related to the grain protein content and application of the primer group, and belongs to the technical field of biology. The wheat-aegilops tauschii introgression line and the secondary segregation population are utilized to finely locate a new major QTL existing in the aegilops tauschii parent and the aegilops tauschii introgression line, and the synergistic gene comes from aegilops tauschii, is not widely utilized by breeders, and has great potential application value. The invention also further develops a KASP molecular marker CS4D-19405835 which is closely linked with the content of the grain protein. High-quality germplasm resources, functional genes and molecular markers are provided for excavation and application of excellent-quality genes of aegilops tauschii, and wheat quality improvement is assisted.

The invention provides a molecular marker of a forest rye powdery mildew resistance related gene PmSESY and application of the molecular marker, and belongs to the technical field of plant molecular biology and genetic breeding. The molecular marker is closely linked with a forest rye powdery mildew resistance gene PmSESY and is marked as PmSESY-M1, and amplification primers of the molecular marker are as shown in SEQ ID No: 1 and SEQ ID No: 2; the molecular marker amplification primer can be subjected to PCR (Polymerase Chain Reaction) amplification in a 1RL chromosome segment material containing the PmSESY gene to obtain a specific product of 383 bp; the molecular marker PmSESY-M1 has the advantages of strong specificity, high stability, good repeatability and the like, can efficiently and accurately detect and track disease-resistant chromosome segments in a wheat-forest rye introgression line, and provides a novel and practical auxiliary selection tool for wheat powdery mildew resistance molecular breeding; the method has an important practical value in wheat powdery mildew resistance molecular breeding.

The invention relates to the technical field of molecular biology, and provides an InDel molecular marker linked with a powdery mildew resistance site PmYZW-6A-1 of a wheat-wheatgrass introgression line and application of the InDel molecular marker. An InDel molecular marker linked with wheat-wheatgrass introgression line powdery mildew resistance sites PmYZW-6A-1 is located at the 231st site to the 257th site of a nucleotide sequence shown in SEQ ID NO.1, and the resistance sites PmYZW-6A-1 are located at 442, 022 and 338bp positions of a 6A chromosome of a common ice 3504 reference genome. According to the technical scheme, the problems that genetic diversity cannot be efficiently utilized and the culture efficiency of broad-spectrum durable disease-resistant varieties is low in a traditional wheat breeding method are solved.