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351 results about "Breeding program" patented technology

A breeding program is the planned breeding of a group of animals or plants, usually involving at least several individuals and extending over several generations. There are a couple of breeding methods, such as artificial (which is man made) and natural (it occurs on its own).

Cry1F and Cry1AC transgenic cotton lines and event-specific identification thereof

This invention relates to plant breeding and the protection of plants from insects. More specifically, this invention includes novel transformation events of cotton plants comprising one or more polynucleotide sequences, as described herein, inserted into specific site(s) within the genome of a cotton cell. In highly preferred embodiments, said polynucleotide sequences encode “stacked” Cry1F and Cry1Ac lepidopteran insect inhibitory proteins. However, the subject invention includes plants having single cry1F or cry1Ac events, as described herein. Additionally, the invention is related to cotton plants derived from that transformation event and to assays for detecting the presence of the event in a sample. More specifically, the present invention provides DNA and related assays for detecting the presence of certain insect-resistance events in cotton. The assays are based on the DNA sequences of recombinant constructs inserted into the cotton genome and of the genomic sequences flanking the insertion sites. These sequences are unique. Based on these insert and border sequences, event-specific primers were generated. PCR analysis demonstrated that these cotton lines can be identified in different cotton genotypes by analysis of the PCR amplicons generated with these event-specific primer sets. Thus, these and other related procedures can be used to uniquely identify these cotton lines. Kits and conditions useful in conducting the assays are also provided. These materials and methods can also be used to assist breeding programs to further develop traits in cotton.
Owner:CORTEVA AGRISCIENCE LLC

Cry1F and Cry1Ac transgenic cotton lines and event-specific identification thereof

This invention relates to plant breeding and the protection of plants from insects. More specifically, this invention includes novel transformation events of cotton plants comprising one or more polynucleotide sequences, as described herein, inserted into specific site(s) within the genome of a cotton cell. In highly preferred embodiments, said polynucleotide sequences encode “stacked” Cry1F and Cry1Ac lepidopteran insect inhibitory proteins. However, the subject invention includes plants having single cry1F or cry1Ac events, as described herein. Additionally, the invention is related to cotton plants derived from that transformation event and to assays for detecting the presence of the event in a sample. More specifically, the present invention provides DNA and related assays for detecting the presence of certain insect-resistance events in cotton. The assays are based on the DNA sequences of recombinant constructs inserted into the cotton genome and of the genomic sequences flanking the insertion sites. These sequences are unique. Based on these insert and border sequences, event-specific primers were generated. PCR analysis demonstrated that these cotton lines can be identified in different cotton genotypes by analysis of the PCR amplicons generated with these event-specific primer sets. Thus, these and other related procedures can be used to uniquely identify these cotton lines. Kits and conditions useful in conducting the assays are also provided. These materials and methods can also be used to assist breeding programs to further develop traits in cotton.
Owner:CORTEVA AGRISCIENCE LLC

Primer group, kit and method for identifying tachysurus fulvidraco and leiocassis longirostris hybrid species

The invention provides a primer group for identifying a male parent and a female parent of tachysurus fulvidraco and leiocassis longirostris hybrid species. The primer group comprises a primer pair for amplifying COI genes and a primer pair for amplifying an ITS sequence, wherein the primer pair for amplifying COI genes consists of a primer COIF and a primer COIR; the primer pair for amplifying an ITS sequence consists of a primer ITSF and a primer ITSR. The invention also provides a kit which is for identifying the male parent and the female parent of tachysurus fulvidraco and leiocassis longirostris hybrid species and comprises the primer group. The kit also comprises any one or more of the following reagents: a buffer solution for PCR, dNTP, heat-resistant DNA polymerase, a cloning vector, a DNA ligase, and a buffer solution for ligation. The invention also provides a method for identifying the male parent and the female parent of tachysurus fulvidraco and leiocassis longirostris hybrid species. The primer group, the kit or the method can be used for accurately identifying the male parent and the female parent of the tachysurus fulvidraco and leiocassis longirostris hybrid species, thus effectively managing a breeding plan and monitoring negative influence thereof, and has great significance in sustainable development of aquaculture industry.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

Wheat double haploid production method by wheat-and-maize distant hybridization

The invention belongs to the field of haploid breeding technology and discloses a wheat double haploid production method by wheat-and-maize distant hybridization. The main technological characteristics of the method comprise steps of planting, induction of haploid embryo, dissection of haploid embryo, respective isolated culture of small embryo and big embryo into haploid plantlet, vernalization,chromosome doubling, transplantation and harvesting. The big embryo is cultured in big embryo culture medium. The small embryo is firstly cultured in small embryo culture medium and then cultured in basal medium. Small embryo culture medium has a higher sugar concentration, a higher osmotic pressure, richer kinds of amino acids, higher vitamin contents and contains trace of growth regulation factors with the germinating and seedling rate of small embryo being over 75%. By low-temperature vernalization, isolated cultured tissue culture seedlings are directly carried out chromosome doubling andplanting with the doubling treatment efficiency being over 89%. The method provided by the invention is adopted to improve seedling rate of haploid embryo and survival rate and success rate of chromosome doubling, simplify wheat breeding program, increase breeding efficiency and shorten breeding cycle, and has apparent effects on accelerating cultivation of fine varieties.
Owner:DRY LAND FARMING INST OF HEBEI ACAD OF AGRI & FORESTRY SCI
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