404 results about "Stichopus" patented technology

The invention discloses a sea cucumber polypeptide, wherein a preparation method thereof comprises the following steps: (1) getting fresh stichopus japonicus, removing entrails, cleaning and crushing the stichopus japonicus into small blocks, and adding water to form homogenate; after the enzymolysis of the homogenate liquid, centrifugating and getting the supernatant; adding ethanol, standing, centrifugating and getting the supernatant, concentrating the supernatant at reducing pressure and drying the concentrate to form a crude product of the sea cucumber polypeptide; (2) dissolving the crude product of the sea cucumber polypeptide by using distilled water, carrying out gel filtration chromatography by Sephadex LH-20, eluting the sea cucumber polypeptide by double-distilled water, getting a second peak, collecting active components, and condensing, freezing and freeze-drying the active components; and (3) dissolving the active components by double-distilled water, carrying out ion exchange chromatography by Q Sepharose Fast Flow, linearly eluting the active components by NaCl solution, desalting, getting the second peak, collecting the active components, and condensing, freeze-drying the active components to form the sea cucumber polypeptide. The sea cucumber polypeptide can be used for preparing medicaments or health-care products for increasing leukocytes and also can be used for preparing medicaments or health-care products for multiplying marrow cells after chemotherapy.

The present invention is bache demer pruning feed and its preparation process. The bache demer pruning feed is prepared with defatted fish meal 4-8 wt%, high gluten flour 12-16 wt%, brewer's yeast 6-10 wt%, algae powder 30-71 wt%, composite vitamins 0.3-0.7 wt% and composite trace elements 0.3-0.7 wt%, where, the algae powder may be replaced with degummed kelp powder 26-30 wt% or sea mud 5-7 wt%. In addition, micro ecological preparation in 0.5 wt% or zeolite powder in 2-4 wt% may be added for good intestinal tract condition of and breeding water environment for bache demer. The bache demer feeding practice shows that the bache demer pruning feed can increase the growth speed of bache demer greatly.

The invention relates to a natural sea area large-scale ecological breeding method of stichopus japonicus, belonging to the field of aquiculture. The method can realize large-scale stichopus japonicus seedling breeding at the natural sea area by the steps of selecting and culturing parent stichopus japonicus, daily management of the parent stichopus japonicus, egg taking and incubating, culturing the young, culturing young stichopus japonicus, classifying offspring seeds and temporary culturing. The method solves the problems of degraded seedling quality, reduced production quality of the seedlings, gradual increment of stichopus japonicus diseases, high energy consumption of a seedling breeding room and the like in the process of seedling cultivation in the existing artificial seedling breeding room, overcomes the technical prejudice that sea cucumber seedlings can not be cultured at the natural sea area in the traditional view, breaks through the inherent thinking set, changes the traditional production way of the artificial seedling breeding room, and can realize ecological breeding in the natural sea area. Furthermore, the method has the remarkable advantages of high survival rate, no pollution and chemical dosing as well as being nuisanceless.

The invention provides a low-temperature vacuum heating and stewing method of sea cucumber. The method comprises the following steps of: a first step of preprocessing fresh sea cucumber; a second step of stewing the preprocessed sea cucumber under a low-temperature vacuum condition; a third step of cooling the stewed sea cucumber under a pressure reduction condition; and a fourth step of processing the cooled sea cucumber according to requirements to obtain a sea cucumber processing product. Compared with the existing stewing and processing method of the sea cucumber, the stewing and processing method is adopted, the sea cucumber appropriately tastes in a relatively-low temperature solution cooking environment under a vacuum stewing condition, and the taste keeps the flavor nearly close to that of the fresh sea cucumber, and nutrient components in the body are kept to the maximum extent, such as stichopus japonicas, proteins and vitamins. By using the method, the problem that the sea cucumber cannot be processed to achieve the effect of appropriate taste under a low temperature condition is completely solved by using a physical method, and no food addictives are added during the processing.

The present invention provides a complete artificial mixed feed for stichopus japonicus. Said feed composition includes (by weight portion) 60-30 portions of sargassum thunbergii powder, 15-25 portions of sea-verdure powder, 15-45 portions of degummed sea-tangle powder, 15-5 portions of spirulina princes powder, 10-5 portions of fermentation yeast, 0.6-0.2 portion of polyvitamin complex, 0.8-0.4 portion of compound mineral salt and 10-60 portions of dried dredge.

The compound feed for stichopus japonicus consists of red fish meal, whey powder, spirulina powder, sage powder, soybean dregs, peanut bran, flour, sea fish oil, soybean lecithin, composite vitamins and composite mineral salt. The compound feed for stichopus japonicus can provide the whole nutrients for stichopus japonicus in artificial raising to result in fast growth of stichopus japonicus, high feed converting rate and high survival rate. As feed for stichopus japonicus, the present invention has no environmental pollution.

The invention discloses a high strength selecting and cultivating method of excellent stichopus japonicus seeds. The high strength selecting and cultivating method comprises the steps of carrying out cultivation, spawning or spermiation promotion, and hybridization on parent stichopus japonicuses from at least four different geographical groups, and carrying out selective breeding at different degrees in different periods according to a quantitative genetics method. By combining two traditional and effective methods of hybridization breeding and selective breeding, the invention preserves excellent properties of the stichopus japonicus and also avoids inbreeding; because the cultivated stichopus japonicuses have excellent properties of different groups, the cultivated stichopus japonicuses have the advantages of fast growth speed, strong stress resistance and the like; and the invention not only realizes seedling pre-culturing of the stichopus japonicus to meet market demand in advance, but also prolongs an effective growth period of the stichopus japonicus within a year, shortens cultivation time, and improves economic benefit; therefore, the inveniton is suitable for large-scaled factory production.

The invention relates to a method for breading Stichopus japonicus seedlings, which belongs to the technical field of Stichopus japonicus culturing and comprises the following steps of screening Stichopus japonicus seedlings, heating and accelerating culturing of the seedlings, culturing at a constant temperature for lying-in, fertilising and hatching the laid eggs, culturing the larva, culturingthe young Stichopus japonicus, aerating, reserving pond and changing sheets. The healthy and high quality Stichopus japonicus seedlings cultured by the method can be matured 2-3 months earlier than that of the Stichopus japonicus seedlings in the natural sea areas, therefore, the thermophilous growth period of the Stichopus japonicus seedlings in the same year can be prolonged; technical measuressuch as water quality regulating and controlling, scientific bait casting, biological disease prevention and the like are adopted in the method to culture the healthy Stichopus japonicus seedlings; with the adoption of the measures such as temperature controlling, light controlling, enforced bait casting and the like, the culturing method can effectively prevent and cure diseases from the culturing of the Stichopus japonicus seedlings to the delivery of the large-scale Stichopus japonicus seedlings, so that high-intensity breeding of the seedlings can be realized and the survival rate from the young Stichopus japonicus to the commercial Stichopus japonicus can reach 80% to 90%.

The invention relates to a traditional Chinese medicine preparation for preventing and curing paralichthys olivaceus and sea cucumber scuticoci liatlda diseases. The preparation comprises the following traditional Chinese medicine components in percentage by weight: 5-20 percent of abrotanum, 5-20 percent of azedarach, 5-20 percent of cyrtomium fortunei, 5-40 percent of stemona, 5-20 percent of golden cypress, 5-20 percent of betel nut and 5-30 percent of omphalia, and the sum of the weight percentages are 100. According to the invention, medicines with components restraining or killing pathogens are selected to make a formula on the basis of traditional Chinese theories, breeding bait is added and absorbed by the host of a parasite, scuticoci liatldas parasitizing in the body or on the body surface of the host of the parasite are completely killed in a body fluid absorbing and supplying way, so that the traditional Chinese medicine preparation has the advantages that medicine feeding is simple, the medicine effect is quick, the insert killing effect is good, the recovery of the physique of the host of the parasite is quick, the survival rate is high, side effects and medicine residue are avoided, as a result, economical loss caused by scuticoci liatlda infection during the production processes of breeding and growing.

The invention provides an ecological cultivation method for the mixed cultivation of the stichopus japonicus and the abalone, relates to the abalone, and provides the ecological cultivation method for the mixed cultivation of the stichopus japonicus and the abalone. The method comprises the steps of: removing the sundries on an abalone cultivating container, and sterilizing the abalone cultivating container when the water temperature of the south sea area is reduced at 19-21 DEG C in autumn annually; putting the stichopus japonicus seeds into the sterilized abalone cultivating container; and feeding the forage for the mixed cultivation of the stichopus japonicus and the abalone. Due to the mixed cultivation of the stichopus japonicus and the abalone, the self cleaning capability of the cultivation water body is furthest enhanced, and the environment condition of the cultivation water body is effectively improved. The stichopus japonicus can largely ingest the residual bait and the excrement generated by the abalone, so that the water body pollution is reduced, the reproduction and the propagation of the pathogene are reduced, and the disease infection of the abalone is reduced. As the stichopus japonicus belongs to the mixed cultivation, the extra cultivation cost does not need to be added, and the cultivation benefit is higher.

The invention relates to a method for culturing a Stichopus japonicus seed, which belongs to the technical field of a Stichopus japonicus seed culturing method and comprises the following steps of (1) fishing and catching; (2) screening; (3) transporting and (4) culturing the Stichopus japonicus seed. According to the method for culturing the Stichopus japonicus seed disclosed by the invention, the culturing process is easy to operate, the Stichopus japonicus seed is scientifically selected and fished and caught in advance; the Stichopus japonicus seed can be matured in advance and lay eggs by utilizing the method of indoor heating and accelerating, thus, large-scale seeds can be cultured in the same year, and the produced Stichopus japonicus seed has enhanced hereditary capacity, strong resistance and good property; moreover, as the feed of the Stichopus japonicus seed and the water temperature and the water quality in the Stichopus japonicus culturing pond are strictly controlled, the fecundity of the Stichopus japonicus is better than that of the Stichopus japonicus cultured in the pond and the intertidal zone, thus, the laid eggs have good quality and the distortion ratio at each stage is high.

The invention discloses a method for preparing stichopus japonicus selenka glycosaminoglycans, which comprises the following steps: (1) carrying out pretreatment on fresh sea cucumber; (2) carrying out alkaline hydrolysis on body wall of the sea cucumber; (3) carrying out dual-enzyme enzymatic hydrolysis on the body wall of the sea cucumber; (4) using trichloroacetic acid for removing proteins in dual-enzyme enzymatic hydrolysis solution after completing the enzymatic hydrolysis; (5) using ethanol for precipitating the stichopus japonicus selenka glycosaminoglycans; (6) drying for obtaining crude stichopus japonicus selenka glycosaminoglycans; (7) using potassium acetate for precipitating the stichopus japonicus selenka glycosaminoglycans; (8) decoloring the stichopus japonicus selenka glycosaminoglycans; (9) using the potassium acetate for precipitating the stichopus japonicus selenka glycosaminoglycans; and (10) drying for obtaining fine stichopus japonicus selenka glycosaminoglycans. The method can balance the relationship between the yield and the purity of the stichopus japonicus selenka glycosaminoglycans and improve the purity of the stichopus japonicus selenka glycosaminoglycans.

The invention relates to the technical field of functional microbiological screening and provides novel Bacillus licheniformis DN29 which is collected under CCTCC NO: M2015482. The Bacillus licheniformis DN29 obtained by screening can effectively inhibit pathogenic bacteria such as Vibrio splendidus and pseudoalteromonas, can reduce disease probability of bred animals and can serve as a feed additive to remarkably increase feed utilization rate of the bred animals and promote growing of the animals. The Bacillus licheniformis DN29 can serve as a probiotic to be applied in the process of rearing and producing Stichopus japonicus, has remarkable promoting effect on growing of Stichopus japonicus and can effectively improve immunity of and resistance, to Vibrio splendidus, of Stichopus japonicus.

The Stichopus japonicus cultivating separate mesh cage apparatus has the structure comprising a frame, mesh sheets and mesh bags. The frame is made of PVC pipes connected with elbows and T-pieces; the mesh sheets are hung inside the frame; and the mesh bags made of polyethylene net are cylinders around the frame and have ends tied with nylon ropes. The separate mesh cage apparatus provides closed Stichopus japonicus growing space without effects of harmful environment factors and enemy organism invasion, and has high Stichopus japonicus survival rate and yield.

A hybridization method between Chinese spiny sea cucumber and Russian (or korean) one for preparing the hybrid one with high growth speed, stress resistance, survival rate and nutritive value includes such steps as using special culture technique to make them mature symultaneously, artificial oxytocia to obtain their spermatozoa and ova, artificial fertilization, and culturing fertilizer ova until they become laval plankton.

The invention discloses a fermentation process of a sea cucumber compound feed, which comprises the following steps: 1-2g of bacillus subtilis at least with the concentration of 1010CFU/g, 20-50ml of lactic acid bacteria at least with the concentration of 109CFU/ml, 0.5-0.8g of yeast at least with the concentration of 1010CFU/g, 5-10g of glucose and 300-400ml of purified water or sterile water are added to a sea cucumber feed, and then acetic acid is used for regulating the pH value to 6.0-6.8; and fermentation is carried out for 12-24 hours at 20-35DEG C. The process is simple, the operation is easy, the production cost is low, and probiotics in the feed are increased, thus promoting digestion of nutrients that are difficult to digest, improving the palatability of the feed, enabling stichopus japonicus to grow rapidly and to have high survival rate and improving the water quality.

The invention relates to a stichopus japonicus powder feed and a processing method thereof and belongs to the technical field of feeds and processing methods thereof. The stichopus japonicus powder feed is characterized by comprising the following ingredients by weight percent: 30-50% of degummed kelp dry powder, 10-20% of sargassum thunbergii dry powder, 15-25% of gulfweed dry powder, 8-13% of perforated sea lettuce dry powder, 5-12% of fish powder, 4-8% of yeast powder and 1-3% of complex vitamin. The processing flow comprises the following steps: collecting raw materials, degumming kelp, drying the raw materials, mixing the raw materials, performing coarse crushing, mixing with the fish powder and stirring, performing superfine crushing and packaging. Nutritional ingredients in the stichopus japonicus powder feed are good in stability, the growth speed and cultivation survival rate of stichopus japonicus can be effectively improved, the growth speed obtained by adopting the stichopus japonicus powder feed is faster than that obtained by adopting other types of the feed by 1.5-2.0 times, the survival rate is high, the weight-increasing rate is fast, and the production efficiencyof cultivation of the stichopus japonicus is greatly improved. Simultaneously, the pollution on a cultivation water body is reduced, and the growth speed of the stichopus japonicus is improved.

The invention relates to a water treatment technique, in particular to a method for biological resource recovery of industrial marine fish aquaculture wastewater. In the method, macroscopic algae, filter feeders, sediment animals and carnivorous fishes are cultured in the industrial marine fish aquaculture wastewater of which the water temperature is between 4 and 27 DEG C to form a biological filter to treat the wastewater discharged by industrial fish aquaculture, wherein the macroscopic algae are warm water macroscopic algae and cold water macroscopic algae; the filter feeders are filtering seashells and fishes, the seashells comprise one or more of oyster, sea-mussel and scallop, and the filtering fishes are barracudas; the carnivorous fishes comprise one or more of weever, flounder and black marine rock fish; and the sediment animals are Stichopus japonicus Selenka. In the method, based on the ecology principle, the macroscopic algae, the filter feeders, the sediment animals and the carnivorous fishes are combined together, and the macroscopic algae, the filter feeders, the sediment animals and the carnivorous fishes coordinate with one another and serve as the biological filter to comprehensively treat the wastewater discharged by the industrial fish aquaculture.

The present invention relates to preparation of two lytic vibrio harveyi bacteriophage preparations, and an application thereof. A purpose of the present invention is to reduce the number of target pathogenic bacteria in the stichopus japonicus body, the stichopus japonicus culture environment and the stichopus japonicus processing products. Host bacteria of the bacteriophage are vibrio harveyi. The two separated bacteriophages of vp-1 and vp-2 provide strong lysis for the vibrio harveyi, and provide protection effects for the stichopus japonicus and the stichopus japonicus products. The preparation of the present invention is a novel green biological disinfection preparation. According to the present invention, the bacteriophage is purified, amplified, cultured and further enriched on a large-scale; because the lytic bacteriophage specifically kills the drug-resistant vibrio harveyi, the problems of the antibiotics resistance due to abuse of antibiotics, antibiotics residue in animal bodies, and the like are prevented; the preparation of the present invention can be prepared into a spray solution or an eluting solution, and provides purification and protection effects for the sick stichopus japonicus, and the aquaculture water environment; the preparation of the present invention does not have any toxic and side-effects, such that the food-derived pollution is reduced, the security effect is provided for the food safety.

The invention discloses a seawater net cage aquaculture method for stichopus japonicus. The seawater net cage aquaculture method comprises the steps of selection of an aquaculture sea area, net cage arrangement, stichopus japonicus fry feeding and aquaculture management. In the selection of the aquaculture sea area, the sea area is required to be 1,000-2,000m off the shore, is high in water quality, does not have a red tide and is rich in natural organic baits; in the net cage arrangement, a net cage rack consists of wood boards and floaters, and 4-8 rows of net cage racks are arranged in the sea in the tide direction; 20-50 net cages are connected in each row; in the stichopus japonicus fry feeding, the specification of selected fries is 60-80 pieces per kilogram, and the density of the fed fries is 4.5-5.0 kilograms per cage; healthy stichopus japonicus fries are selected; the aquaculture management comprises four steps of feeding baits, preventing harmful organisms, cleaning impurities and setting a warning device. The seawater net cage aquaculture method is convenient to use; by net cage aquaculture, the possibility that the stichopus japonicus is injured can be reduced, the survival rate of the stichopus japonicus is increased, the yield of the stichopus japonicus is improved, and the demand of the market can be met.

The invention relates to a sea cucumber feed prepared from alfalfa and a preparation method thereof. The sea cucumber feed comprises the following components in percentage by weight: 25-36 percent of alfalfa, 10-22 percent of bean pulp, 8-14 percent of peanut meal, 4-15 percent of corn meal, 1-8 percent of gluten meal, 0-12 percent of seaweed meal, 2-8 percent of fish meal, 8-15 percent of scallop skirt meal, 2-15 percent of shrimp meal, 2-10 percent of yeast powder, 1-4 percent of complex vitamins special for aquiculture and 1-2 percent of mineral substances special for aquiculture. The preparation method comprises the following steps of: (1) weighing the raw materials according to the weight percentage of each component; (2) mixing and roughly crushing the weighed raw materials; (3) carrying out ultramicro crushing on the roughly-crushed materials; and (4) selecting a sieve according to the body size of the sea cucumbers and sieving the materials. The preparation method has a scientific and reasonable formula and a simple and convenient process; and the prepared feed has an appropriate calcium-phosphorus rate and has the effects of remarkably promoting the growth of the sea cucumbers, shorting the culturing cycle and improving the stress resistance capability and the capabilities of saving a great amount of seaweed resources and reducing the pollution to the water body.

The invention discloses a method for extracting active components from stichopus japonicus and extracts obtained by the method. The extraction method comprises the following step of performing ultrasonic treatment on stichopus japonicus slurry, wherein the treatment temperature is 0-10 DEG C. According to the method, stichopus japonicus unsaturated fatty acid, vitamin E, and the like with high liposoluble properties, and active substances of stichopus japonicus collagen, stichopus japonicus polysaccharide, stichopus japonicus polypeptide and the like, in stichopus japonicus are sufficiently extracted as far as possible, so that stichopus japonicus resources are used to the maximum extent, and besides, micromolecular pollutants of heavy metal ions and the like, remaining in the bodies of the stichopus japonicus are removed; and therefore, a mild physical and biological method is used, so that the activity of effective components in the stichopus japonicus is reserved to the maximum extent, and the condition that the active components run off and the activity of the active components is changed in the processing course is avoided.