18 results about "Stichopus" patented technology

The invention discloses a seedling breeding and under-forest cultivation method for Schefflera octophylla. Relates to the technical field of medicinal plant cultivation. Comprising the following steps: seed treatment; sowing and germinating; seedling cultivation; performing under-forest field planting; and managing after planting. The germination rate is high and neat; seedlings are robust and strong in stress resistance; the field planting survival rate is obviously improved, and the under-forest field planting survival rate is stabilized to be 85% or above; the whole technical route does not need to use chemical pesticides and chemical fertilizers, the Yunnan pine forest land resources are utilized, bionic cultivation of the stichopus japonicus is achieved, the ecological environment is protected, and technical support is provided for production of high-quality medicinal materials of the stichopus japonicus.

This invention discloses a method for preparing the multifunctional bioactive peptide Aj-UBL from sea cucumber, relating to the fields of biotechnology and biomedicine. Using the Aj-UBL coding sequence from sea cucumber as a template, the method involves PCR amplification, recombinant expression vector construction, engineered bacterial induction expression, bacterial cell disruption, affinity chromatography purification, and identification to obtain the target peptide possessing triple activities of antibacterial, antiprotozoal, and antitumor activity. This invention employs an optimized coupling process of genetic engineering and affinity chromatography to achieve high-purity, high-quality, and stable product preparation, while being environmentally friendly and free from drug resistance risks. It not only solves the problem of coordinated disease control in sea cucumber farming but also expands the high-value application of sea cucumber resources in the biomedical field, filling the technological gap in the large-scale preparation of multifunctional bioactive peptides from sea cucumber and the biological control of ciliates, possessing significant theoretical and practical application value.

The utility model relates to the technical field of mixing machines, and discloses a stichopus japonicus active peptide enzymolysis freeze-dried powder auxiliary material mixing machine which comprises a material mixing tank installed on a machine base, the two ends of the material mixing tank are open, a charging tank is connected into the material mixing tank, one end of the charging tank is fixedly connected and communicated with a tank opening, and the other end of the charging tank is fixedly connected with a stirrer. The side, away from the charging tank, of the tank opening is connected with a tank cover. According to the stichopus japonicus active peptide enzymolysis freeze-dried powder auxiliary material mixing machine, the charging tank is installed in the material mixing tank, after material mixing is completed, the charging tank can be pulled out of the material mixing tank, a new charging tank is installed for mixing processing, and the mixing processing efficiency of stichopus japonicus active peptide enzymolysis freeze-dried powder auxiliary materials is improved.

The invention relates to a seawater stichopus japonicus culture pond environment regulation and control method based on a bottom nourishing device and a mixed microbial agent and application. According to the method, the seawater stichopus japonicus culture pond is subjected to bottom nourishing operation, meanwhile, mixed microbial agents of rhodobacter sphaeroides, phosphorus phagocytosis bacillus, moraxella alpha and enterococcus faecalis are splashed, the water quality of the stichopus japonicus culture pond is improved, meanwhile, the immune function of stichopus japonicus is enhanced, and remarkable environmental and economic benefits are achieved.

The invention discloses a sea cucumber feed as well as a preparation method and application thereof, and belongs to the technical field of sea cucumber feed preparation. According to the technical scheme, every 100 g of feed comprises 40 g of sea mud, 15-25 g of gulfweed powder, 5-15 g of enzymolysis gulfweed liquid and 1 g of multi-vitamin multi-mineral premix. The dosage of the enzymolysis gulfweed liquid is calculated according to the dry weight of the gulfweed, and the enzymolysis gulfweed liquid is obtained by performing enzymolysis on the gulfweed through algin degrading enzyme, pectinase and cellulase. The sea cucumber feed is applied to the aspect of sea cucumber feeding, solves the problem that the existing sea cucumber feed still cannot well promote accumulation of sea cucumber saponin and fucose in the body wall of the sea cucumber so as not to improve the nutritional value of the sea cucumber, and can effectively promote accumulation of sea cucumber saponin and fucose in the body wall of the sea cucumber so as to improve the nutritional value of the sea cucumber.

The utility model discloses a water quality regulation and control device for ecological culture of stichopus japonicus. The water quality regulation and control device comprises aeration equipment and a cleaning mechanism, the aeration equipment comprises an air compressor, a connecting pipe and an aerator, the aerator is connected with the air compressor through the connecting pipe, and a plurality of ventilation holes are formed in the aerator; the cleaning mechanism comprises a circular bottom plate and a cleaning scraping block used for cleaning impurities attached to the interior of the ventilation hole, the circular bottom plate is placed in the aerator, and the cleaning scraping block is arranged in the ventilation hole. Under the action of wind, an arc-shaped baffle is pushed to move up and down, under the connection of an arc-shaped push plate, a circular frame is driven to move up and down, meanwhile, a cleaning scraping block is driven to move up and down in a ventilation hole, impurities attached to the inner wall of the ventilation hole can be scraped and cleaned, and the situation that the ventilation effect of the aerator is affected due to the fact that the ventilation hole is blocked by the impurities in water is avoided.

This invention relates to a land-based sea cucumber culture pond and a method for cultivating sea cucumbers. The land-based culture pond comprises: several culture units, each including a net reef and pond water near the reef for sea cucumber cultivation; and several heat exchange pipes, each including a third pipe laid near the culture unit and cold water within the third pipe. The cold water flowing through the heat exchange pipes lowers the temperature of the pond water near the net reef by 2-5°C. This invention solves the problem of how to regulate the bottom water temperature of the land-based sea cucumber culture pond, thereby improving the survival rate of sea cucumbers during summer dormancy.

This invention belongs to the field of marker-assisted breeding technology, and relates to the combination and application of SNP molecular markers for intestinal regeneration in *S. spinosaurus*. This invention uses the ratio of open intestinal length to total intestinal length during intestinal regeneration as a continuous trait indicator of intestinal regeneration capacity. Using whole-genome resequencing data and the BLINK model, genome-wide association analysis was performed to screen for 22 SNP molecular markers significantly associated with intestinal regeneration capacity in *S. spinosaurus*. The nucleotide sequences corresponding to these SNP molecular markers are shown in SEQ ID NO. 1-22, each sequence being 200 bp in length, with the SNP site located at the 100th bp. By detecting the genotype of these SNP sites, the intestinal regeneration capacity of *S. spinosaurus* can be evaluated at the molecular level, and superior individuals with faster intestinal regeneration can be screened for use in stress-resistant germplasm screening, marker-assisted breeding, and the construction of high-regeneration-capacity *S. spinosaurus* breeding populations.

The utility model relates to sea cucumber seedling breeding technical field, and disclose a kind of ecological aquaculture pond of sea cucumber seedling disease prevention and control, including aquaculture pond, and the prevention and control assembly of setting at the top of aquaculture pond, the prevention and control assembly includes the U-shaped frame slidingly connected in the top of aquaculture pond, the outer wall of U-shaped frame is fixedly connected with forward and reverse motor, the output of forward and reverse motor is fixedly connected with screw rod, the other end of screw rod is rotatably connected with the inner wall of U-shaped frame, the outer wall of screw rod is threadedly connected with sleeve block, the top of sleeve block is fixedly connected drive motor, the top outer wall of sleeve block is fixedly connected with annular frame, the output of drive motor is fixedly connected with driving shaft, the top outer wall of driving shaft is fixedly connected with driving gear, the side wall of driving gear is engaged with driven gear, by setting prevention and control assembly, it is convenient to drive pest lamp to drive away pest, to reach the prevention and control effect in the process of sea cucumber seedling cultivation.

The application discloses a whole-genome SNP molecular marker combination related to the apraocha of sea cucumber against vibrio splendidus and application thereof, wherein the SNP molecular marker combination comprises 35 SNP sites, and in disease-resistant breeding, individuals with the 35 SNP site dominant genotypes can be screened to accelerate the breeding process of the disease-resistant traits. The molecular marker provided by the application has high biological correlation and prediction accuracy in breeding, and can be screened through DNA detection without relying on the phenotype of individual infection and disease, so that the generation interval is greatly shortened, the breeding efficiency is improved, the molecular marker assisted selection can overcome environmental interference, the genotypes are directly selected, the selection strength is high, the accuracy is high, and the genetic progress of the disease-resistant traits is accelerated.

The application provides a sea cucumber breeding bait feeding device and a dirt removing system, and relates to the field of sea cucumber breeding.The device comprises a bottom plate, a net cage and a mounting frame, a plurality of thin rods are arranged at the bottom of the bottom plate, a scattering box and a motor are arranged at the top of the bottom plate, a transmission shaft is arranged between the motor and the scattering box, a plurality of stirring blades and a plurality of impact plates are fixedly connected to the outer side of the transmission shaft.The vibrating air bag shakes the internal bait, and the bait is spread on the inside of the air bag and falls into the net cage through the expanded discharge chute.The plurality of discharge chutes of the air bag are evenly distributed on the top of the net cage, so that the bait falling through the discharge chute is evenly distributed in each part of the net cage, the sea cucumber in the sea cucumber breeding tank is prevented from moving and accumulating in a small place due to eating bait, the lack of oxygen of the sea cucumber is avoided, and the survival rate of the large-scale breeding of the sea cucumber is ensured.

The invention belongs to the technical field of preparation of fermented feeds, and discloses a preparation method of an alginic acid degrading bacterium fermented feed of apostichopus japonicus. The alginic acid is converted into the alginate oligosaccharide by fermenting the basal feed through bacillus weihaensis, so that the absorptivity and the active components of the feed are improved. According to the prepared fermented feed, the residual amount of alginic acid is reduced, the content of crude protein is increased, then the weight gain rate, the specific growth rate and the feed conversion rate of the fed stichopus japonicus are remarkably increased, and the absorption and utilization capacity of the stichopus japonicus to feed nutrient substances is improved. The activity of intestinal digestive enzymes of the stichopus japonicus and the activity of immune-related enzymes are improved. And the microbial community structure of the intestinal tract of the stichopus japonicus is obviously changed. The growth performance and the disease resistance of the stichopus japonicus are improved. The breeding cost is reduced and the environmental pollution is reduced.

The application provides application of indole-3-lactic acid in improving immunity of sea cucumber and inhibiting virulence factors of Vibrio splendidus. The application is used for preparing a product for inhibiting virulence factors of Vibrio, and a product for improving immune defense ability of aquatic animals; wherein the aquatic animals are preferably sea cucumber, and the Vibrio is preferably Vibrio splendidus. Researches show that under the premise of basically not affecting the growth of Vibrio splendidus, the indole-3-lactic acid can significantly reduce hemolytic activity, protease activity, motility and biofilm formation of the Vibrio splendidus, and can reduce intestinal permeability of the sea cucumber, repair intestinal physical barrier, relieve excessive inflammatory response induced by Vibrio splendidus infection, thereby improving survival rate of the sea cucumber after attack and reducing incidence probability. The technology has the advantages of small dosage, clear effect, green safety and low drug resistance selection pressure, and is suitable for prevention and control of sea cucumber skin rot syndrome.

The present application relates to sea cucumber aquaculture technical field, especially to a kind of sea cucumber seedling cultivation method based on vibrio regulation.The cultivation method is in the whole process of sea cucumber seedling cultivation according to the total number of bacteria and the total number of vibrio in the detected environment, the total number of bacteria / vibrio total number ratio is calculated, and the amount of probiotics is adjusted according to the total number of bacteria / vibrio total number ratio, compared with sea cucumber seedling without adding probiotics, the yield of sea cucumber seedling is increased by more than 50%, and the survival rate is increased by more than 10%.

The application provides a sea cucumber origin tracing model construction method and a sea cucumber origin identification method based on element content and stable isotopes, and relates to the technical field of aquatic product origin tracing. 13 C, δ 15 N, δ 2 H and δ 18 O values of the sea cucumber samples from different origins are determined to obtain determination data of the sea cucumber samples from different origins; linear discriminant analysis is performed with the determination data as independent variables and the origins as observation variables to obtain an LDA model, and the LDA model is a sea cucumber origin tracing model. The technical scheme provided by the application can efficiently and accurately realize sea cucumber origin tracing, and has the advantages of simple operation, low cost, accurate classification results, strong interpretability and the like, and has high application value and popularization prospect.

The invention discloses an intermediate cultivation method for stichopus japonicus fry, and belongs to the technical field of aquatic animal fry cultivation. According to the technical scheme, the method comprises the following steps: uniformly spraying 5000-6000 heads per jin of ginseng seedlings, 10000-20000 heads per jin of ginseng seedlings and 5000-60000 heads per jin of ginseng seedlings onto a three-dimensional layered flat net structure; small-sized sea cucumber seedlings are put on the three-dimensional layered flat net structure in the middle of the pond, large-sized sea cucumber seedlings are put on the three-dimensional layered flat net structures on the two sides of the pond, and the sea cucumber seedling putting density in the middle of the three-dimensional layered flat net structure is higher than the sea cucumber seedling putting density at the two ends of the three-dimensional layered flat net structure; the three-dimensional layered flat net structure comprises three layers of flat nets which are sequentially arranged from top to bottom, a plurality of flat nets are parallelly arranged in the pond at intervals, two cage net groups are parallelly arranged on each of two sides of each flat net, and the two cage net groups are arranged between the adjacent flat nets at intervals; and each cage net group consists of three cage nets which are arranged in the shape of a Chinese character'pin '. The method is applied to the aspect of cultivation of the stichopus japonicus seedlings, labor can be saved, and the yield and quality of the stichopus japonicus seedlings can be stably guaranteed.

The present application provides a method and application of 5-iodoindole inhibiting virulence factors of Vibrio splendidus, and it is found that different concentrations of 5-iodoindole can significantly reduce the expression of virulence factors such as hemolytic activity and protease activity of Vibrio splendidus under the premise of basically not affecting the growth of Vibrio splendidus. Through the method, the infection toxicity of Vibrio splendidus can be weakened, so as to reduce the infection and disease probability of host sea cucumber and alleviate the infection symptoms. Meanwhile, the present application also provides the application of 5-iodoindole in reducing the expression of Vibrio splendidus related virulence genes and the application of 5-iodoindole in improving the survival rate of host sea cucumber after Vibrio splendidus attack, which proves that 5-iodoindole with a concentration of 20 muM has guiding significance for actual sea cucumber production and culture. And through the research on virulence factors of Vibrio splendidus, the present application is helpful to reduce the infection and disease of sea cucumber caused by Vibrio splendidus.

The invention relates to the technical field of biology, and particularly discloses fungus and diatom symbiont microspheres for improving the immune function of cage culture stichopus japonicus and improving water quality. Core functional components of the fungus and diatom symbiont microspheres are cordyceps militaris fungi and Ampelopsis coffee, and in addition, in order to improve the biological activity of the symbiont microspheres and the adhesion of the symbiont microspheres to a netting, pullulan, recombinant mussel mucin, a folium cortex eucommiae extract and a curcuma zedoary extract are also added in the preparation process of the microspheres. The functional components of the symbiont microsphere disclosed by the invention are mutually beneficial, common and synergistic, the non-specific immune function of the stichopus japonicus can be comprehensively improved by enhancing the activity of immune enzyme and digestive enzyme, and meanwhile, the contents of nitrogen phosphorus, nitrite, organic pollutants and pathogenic vibrio in an aquaculture water body can also be reduced.