9 results about "Stichopus" patented technology

This invention discloses a method for preparing the multifunctional bioactive peptide Aj-UBL from sea cucumber, relating to the fields of biotechnology and biomedicine. Using the Aj-UBL coding sequence from sea cucumber as a template, the method involves PCR amplification, recombinant expression vector construction, engineered bacterial induction expression, bacterial cell disruption, affinity chromatography purification, and identification to obtain the target peptide possessing triple activities of antibacterial, antiprotozoal, and antitumor activity. This invention employs an optimized coupling process of genetic engineering and affinity chromatography to achieve high-purity, high-quality, and stable product preparation, while being environmentally friendly and free from drug resistance risks. It not only solves the problem of coordinated disease control in sea cucumber farming but also expands the high-value application of sea cucumber resources in the biomedical field, filling the technological gap in the large-scale preparation of multifunctional bioactive peptides from sea cucumber and the biological control of ciliates, possessing significant theoretical and practical application value.

This invention belongs to the field of marker-assisted breeding technology, and relates to the combination and application of SNP molecular markers for intestinal regeneration in *S. spinosaurus*. This invention uses the ratio of open intestinal length to total intestinal length during intestinal regeneration as a continuous trait indicator of intestinal regeneration capacity. Using whole-genome resequencing data and the BLINK model, genome-wide association analysis was performed to screen for 22 SNP molecular markers significantly associated with intestinal regeneration capacity in *S. spinosaurus*. The nucleotide sequences corresponding to these SNP molecular markers are shown in SEQ ID NO. 1-22, each sequence being 200 bp in length, with the SNP site located at the 100th bp. By detecting the genotype of these SNP sites, the intestinal regeneration capacity of *S. spinosaurus* can be evaluated at the molecular level, and superior individuals with faster intestinal regeneration can be screened for use in stress-resistant germplasm screening, marker-assisted breeding, and the construction of high-regeneration-capacity *S. spinosaurus* breeding populations.

The utility model relates to sea cucumber seedling breeding technical field, and disclose a kind of ecological aquaculture pond of sea cucumber seedling disease prevention and control, including aquaculture pond, and the prevention and control assembly of setting at the top of aquaculture pond, the prevention and control assembly includes the U-shaped frame slidingly connected in the top of aquaculture pond, the outer wall of U-shaped frame is fixedly connected with forward and reverse motor, the output of forward and reverse motor is fixedly connected with screw rod, the other end of screw rod is rotatably connected with the inner wall of U-shaped frame, the outer wall of screw rod is threadedly connected with sleeve block, the top of sleeve block is fixedly connected drive motor, the top outer wall of sleeve block is fixedly connected with annular frame, the output of drive motor is fixedly connected with driving shaft, the top outer wall of driving shaft is fixedly connected with driving gear, the side wall of driving gear is engaged with driven gear, by setting prevention and control assembly, it is convenient to drive pest lamp to drive away pest, to reach the prevention and control effect in the process of sea cucumber seedling cultivation.

The application discloses a whole-genome SNP molecular marker combination related to the apraocha of sea cucumber against vibrio splendidus and application thereof, wherein the SNP molecular marker combination comprises 35 SNP sites, and in disease-resistant breeding, individuals with the 35 SNP site dominant genotypes can be screened to accelerate the breeding process of the disease-resistant traits. The molecular marker provided by the application has high biological correlation and prediction accuracy in breeding, and can be screened through DNA detection without relying on the phenotype of individual infection and disease, so that the generation interval is greatly shortened, the breeding efficiency is improved, the molecular marker assisted selection can overcome environmental interference, the genotypes are directly selected, the selection strength is high, the accuracy is high, and the genetic progress of the disease-resistant traits is accelerated.

The application provides application of indole-3-lactic acid in improving immunity of sea cucumber and inhibiting virulence factors of Vibrio splendidus. The application is used for preparing a product for inhibiting virulence factors of Vibrio, and a product for improving immune defense ability of aquatic animals; wherein the aquatic animals are preferably sea cucumber, and the Vibrio is preferably Vibrio splendidus. Researches show that under the premise of basically not affecting the growth of Vibrio splendidus, the indole-3-lactic acid can significantly reduce hemolytic activity, protease activity, motility and biofilm formation of the Vibrio splendidus, and can reduce intestinal permeability of the sea cucumber, repair intestinal physical barrier, relieve excessive inflammatory response induced by Vibrio splendidus infection, thereby improving survival rate of the sea cucumber after attack and reducing incidence probability. The technology has the advantages of small dosage, clear effect, green safety and low drug resistance selection pressure, and is suitable for prevention and control of sea cucumber skin rot syndrome.

The application provides a sea cucumber origin tracing model construction method and a sea cucumber origin identification method based on element content and stable isotopes, and relates to the technical field of aquatic product origin tracing. 13 C, δ 15 N, δ 2 H and δ 18 O values of the sea cucumber samples from different origins are determined to obtain determination data of the sea cucumber samples from different origins; linear discriminant analysis is performed with the determination data as independent variables and the origins as observation variables to obtain an LDA model, and the LDA model is a sea cucumber origin tracing model. The technical scheme provided by the application can efficiently and accurately realize sea cucumber origin tracing, and has the advantages of simple operation, low cost, accurate classification results, strong interpretability and the like, and has high application value and popularization prospect.

The present application provides a method and application of 5-iodoindole inhibiting virulence factors of Vibrio splendidus, and it is found that different concentrations of 5-iodoindole can significantly reduce the expression of virulence factors such as hemolytic activity and protease activity of Vibrio splendidus under the premise of basically not affecting the growth of Vibrio splendidus. Through the method, the infection toxicity of Vibrio splendidus can be weakened, so as to reduce the infection and disease probability of host sea cucumber and alleviate the infection symptoms. Meanwhile, the present application also provides the application of 5-iodoindole in reducing the expression of Vibrio splendidus related virulence genes and the application of 5-iodoindole in improving the survival rate of host sea cucumber after Vibrio splendidus attack, which proves that 5-iodoindole with a concentration of 20 muM has guiding significance for actual sea cucumber production and culture. And through the research on virulence factors of Vibrio splendidus, the present application is helpful to reduce the infection and disease of sea cucumber caused by Vibrio splendidus.

The invention relates to the technical field of biology, and particularly discloses fungus and diatom symbiont microspheres for improving the immune function of cage culture stichopus japonicus and improving water quality. Core functional components of the fungus and diatom symbiont microspheres are cordyceps militaris fungi and Ampelopsis coffee, and in addition, in order to improve the biological activity of the symbiont microspheres and the adhesion of the symbiont microspheres to a netting, pullulan, recombinant mussel mucin, a folium cortex eucommiae extract and a curcuma zedoary extract are also added in the preparation process of the microspheres. The functional components of the symbiont microsphere disclosed by the invention are mutually beneficial, common and synergistic, the non-specific immune function of the stichopus japonicus can be comprehensively improved by enhancing the activity of immune enzyme and digestive enzyme, and meanwhile, the contents of nitrogen phosphorus, nitrite, organic pollutants and pathogenic vibrio in an aquaculture water body can also be reduced.