61 results about "Stichopus" patented technology

The invention belongs to the technical field of microorganisms, and discloses lactobacillus rhamnosus capable of relieving toxicity of heavy metal cadmium and application of the lactobacillus rhamnosus. The lactobacillus rhamnosus is preserved in the China Center for Type Microbiological Culture Collection, the preservation address is Wuhan University, Hubei province, and the preservation number is CCTCC No.M20241815. The lactobacillus rhamnosus is named lactobacillus rhamnosus. The lactobacillus rhamnosus provided by the invention is derived from intestinal tracts of healthy stichopus japonicus, has good heavy metal cadmium tolerance and removal capacity, and is expected to be developed and applied to the healthy food industry.

The utility model relates to an artificial fish reef stichopus japonicus proliferation catching technology, in particular to a stichopus japonicus trapping device for an artificial fish reef area. The device adopts a net cage structure, a net cage frame is made of corrosion-resistant metal profiles or PE profiles, soft nylon nettings are fixed on the net cage frame to form the bottom of the net cage and the periphery of the cage body, and movable doors which are movably connected and can be opened and closed are arranged on the periphery of the cage body of the net cage, so that the stichopus japonicus in an artificial fish reef area can enter the cage body. According to the arrangement form and scale of an artificial fish reef area, fixed-point pile arrangement and throwing of the stichopus japonicus catching device are carried out, the stichopus japonicus catching device can be independently used and can be arranged in rows for use, arrangement is convenient, use is flexible, and the stichopus japonicus catching device is suitable for being used in various different artificial fish reef construction sea areas.

The present invention belongs to the field of traditional Chinese medicine technology and primarily relates to a traditional Chinese medicine composition, preparation method, and application for preventing and treating sea cucumber rotten skin syndrome. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 20-80 parts of Terminalia chebula, 3-20 parts of Galla chinensis, 10-50 parts of Andrographis paniculata, and 5-30 parts of Ligusticum chuanxiong. The traditional Chinese medicine composition can significantly enhance the immune function and disease resistance of sea cucumbers, reduce the inflammatory response and pathological damage caused by Vibrio splendens, slow the progression of sea cucumber disease caused by pathogenic bacteria, and reduce the morbidity and mortality of sea cucumbers suffering from rotten skin syndrome, thereby effectively preventing and treating sea cucumber rotten skin syndrome.

The invention discloses a stichopus japonicus fry cultivation method, and belongs to the technical field of stichopus japonicus fry cultivation. Comprising the following steps: splashing a fermented feed containing a bacillus subtilis fermentation liquid and a bacterial liquid containing an enterococcus faecalis fermentation liquid and a lactobacillus casei fermentation liquid into a culture pond of which the pond bottom is paved with a polyethylene corrugated plate substratum; the fermented feed and the bacterial liquid are continuously splashed until the water quality in the culture pond is clear and transparent, the feed on the corrugated plate is fluffy and is in a short villus shape, and a stable beneficial bacterium phase is formed; peeling off the juvenile sea cucumbers on the 40th day after metamorphosis and attachment, distributing the juvenile sea cucumbers into a cultivation pond for cultivation, and feeding fermented feed and bacterial liquid every day. The method is applied to the aspect of stichopus japonicus fry cultivation, the problems that in existing stichopus japonicus fry production, diseases occur frequently, most fry breeding fields face poor fry quality, and the yield is low are solved, a stable cultivation environment dominated by beneficial bacteria is built, external multi-dimensional disease sources are buffered, blocked and eliminated continuously, and the survival rate of the stichopus japonicus fry is increased. The stichopus japonicus seedlings can grow in the environment without stress.

The invention provides a frozen section method for space transcriptomics analysis of mesentery and regenerative intestinal tract of stichopus japonicus, and belongs to the technical field of biotechnology. The method comprises the steps of sample treatment and embedding, pre-cooling by a freezing slicer, freezing and slicing, and flaking. According to the frozen section method for space transcriptome analysis of the mesentery and the regenerative intestinal tract of the stichopus japonicus, provided by the invention, a fresh material is directly embedded, and a plurality of thin tissue blocks with the same size are integrated into a standard tissue block of 1cm, so that the high coverage rate of a tissue cross section in a space transcriptome chip capture area is ensured; rNA degradation is effectively prevented, and finally, the cDNA library is successfully constructed and is used for subsequent analysis of a space transcriptome. The technical scheme has the characteristics of simplicity and convenience in operation and stable effect, and is particularly suitable for space transcriptomics frozen section preparation of thin film type tissues.

The invention discloses a seedling breeding and under-forest cultivation method for Schefflera octophylla. Relates to the technical field of medicinal plant cultivation. Comprising the following steps: seed treatment; sowing and germinating; seedling cultivation; performing under-forest field planting; and managing after planting. The germination rate is high and neat; seedlings are robust and strong in stress resistance; the field planting survival rate is obviously improved, and the under-forest field planting survival rate is stabilized to be 85% or above; the whole technical route does not need to use chemical pesticides and chemical fertilizers, the Yunnan pine forest land resources are utilized, bionic cultivation of the stichopus japonicus is achieved, the ecological environment is protected, and technical support is provided for production of high-quality medicinal materials of the stichopus japonicus.

The invention relates to a preparation method of apostichopus japonicus collagen peptide, which comprises the following steps: solid-phase synthesis of apostichopus japonicus collagen peptide is carried out, and the amino acid sequence of the apostichopus japonicus collagen peptide is shown as SEQ ID NO: 1. The apostichopus japonicus collagen peptide prepared by the method disclosed by the invention is an apostichopus japonicus collagen peptide with antioxidant activity and hypoglycemic activity.

This invention discloses a method for preparing the multifunctional bioactive peptide Aj-UBL from sea cucumber, relating to the fields of biotechnology and biomedicine. Using the Aj-UBL coding sequence from sea cucumber as a template, the method involves PCR amplification, recombinant expression vector construction, engineered bacterial induction expression, bacterial cell disruption, affinity chromatography purification, and identification to obtain the target peptide possessing triple activities of antibacterial, antiprotozoal, and antitumor activity. This invention employs an optimized coupling process of genetic engineering and affinity chromatography to achieve high-purity, high-quality, and stable product preparation, while being environmentally friendly and free from drug resistance risks. It not only solves the problem of coordinated disease control in sea cucumber farming but also expands the high-value application of sea cucumber resources in the biomedical field, filling the technological gap in the large-scale preparation of multifunctional bioactive peptides from sea cucumber and the biological control of ciliates, possessing significant theoretical and practical application value.

The present invention relates to an ecological cage aquaculture method. The method proposes a cage-based relay aquaculture model for sea cucumbers, sea urchins, and fish, wherein sea cucumbers and sea urchins are mixed in cages during the wintering period, and are combined with green pufferfish aquaculture in the summer. This achieves the comprehensive utilization of cage aquaculture, effectively avoids the risks of aquaculture of sea cucumbers and sea urchins during the high temperature period in summer, saves feeding bait, and, based on the biological characteristics of green pufferfish that feed on mollusks, removes cage attachments in a targeted manner, achieving a biological cycle and biocontrol of pollutants in marine aquaculture, reducing the labor intensity of subsequent cleaning of cage-fouling organisms. The method adopts a "well"-shaped HDPE grid cage to greatly improve the utilization efficiency of the aquaculture device. The method is equipped with an automatic feeding system to solve the problem of difficult and dangerous feeding in large cages. The method feeds bait in a scientific and standardized manner, saving feeding time and manpower input, reducing labor intensity, and greatly improving labor output efficiency.

The utility model relates to the technical field of mixing machines, and discloses a stichopus japonicus active peptide enzymolysis freeze-dried powder auxiliary material mixing machine which comprises a material mixing tank installed on a machine base, the two ends of the material mixing tank are open, a charging tank is connected into the material mixing tank, one end of the charging tank is fixedly connected and communicated with a tank opening, and the other end of the charging tank is fixedly connected with a stirrer. The side, away from the charging tank, of the tank opening is connected with a tank cover. According to the stichopus japonicus active peptide enzymolysis freeze-dried powder auxiliary material mixing machine, the charging tank is installed in the material mixing tank, after material mixing is completed, the charging tank can be pulled out of the material mixing tank, a new charging tank is installed for mixing processing, and the mixing processing efficiency of stichopus japonicus active peptide enzymolysis freeze-dried powder auxiliary materials is improved.

The present application belongs to the technical field of marine ecological breeding, and particularly relates to a compound marine ranching device and method based on urchin-alga-stichopus nutrient internal circulation. The compound marine ranching device based on urchin-alga-stichopus nutrient internal circulation comprises an urchin breeding area, a stichopus breeding area, a reef-throwing bottom sowing area, an environment monitoring and control system and a main body connecting frame. The urchin breeding area and the stichopus breeding area are both provided with multiple net cages, and the urchin net cages and the stichopus net cages are staggered. The reef-throwing bottom sowing area is located at the lower part of the breeding area. The environment monitoring and control system comprises a sensor group, a remote monitoring host and a control execution unit. The compound marine ranching device and method based on urchin-alga-stichopus nutrient internal circulation can absorb nitrogen and phosphorus generated in breeding by means of algae, and improve the bottom material by stichopus disturbance, thereby relieving the problems of eutrophication and bottom layer anoxia from the source, and greatly improving the breeding yield and economic benefits per unit sea area.

The invention relates to a seawater stichopus japonicus culture pond environment regulation and control method based on a bottom nourishing device and a mixed microbial agent and application. According to the method, the seawater stichopus japonicus culture pond is subjected to bottom nourishing operation, meanwhile, mixed microbial agents of rhodobacter sphaeroides, phosphorus phagocytosis bacillus, moraxella alpha and enterococcus faecalis are splashed, the water quality of the stichopus japonicus culture pond is improved, meanwhile, the immune function of stichopus japonicus is enhanced, and remarkable environmental and economic benefits are achieved.

The invention relates to a soybean clear water feed capable of remarkably promoting growth of stichopus japonicus and a preparation method and application of the soybean clear water feed, and the soybean clear water feed comprises the following components in parts by mass: 20-40 parts of lactobacillus fermented soybean clear water and 100 parts of stichopus japonicus basal feed. The lactobacillus fermented soybean clear water is obtained by adding lactobacillus bulgaricus into the soybean clear water for fermentation; the preparation method comprises the following steps: fully mixing a stichopus japonicus basal feed, crushing, filtering and screening by using a 100-mesh screen, adding lactobacillus fermented soybean clear water, fully and uniformly mixing, granulating and drying. The soybean clear water stichopus japonicus feed is suitable for stichopus japonicus culture modes such as indoor ponds, outdoor ponds and outdoor net cages. By using the bean clear water, the environmental pollution is reduced, and the feed production cost is reduced; meanwhile, the nutrition is rich, macromolecular proteins, phytic acid, trypsin inhibitors and other anti-nutritional factors in the soybean clear water are fully degraded in the lactic acid bacteria fermentation process, the intestinal flora of the stichopus japonicus can be optimized, the feed utilization rate is increased, the weight gain of the stichopus japonicus is promoted, and the antioxidant capacity of the stichopus japonicus is enhanced.

This invention patent belongs to the field of aquaculture equipment and relates to an automatic sea cucumber seedling transfer machine used during the sea cucumber seedling rearing process for transferring seedlings to different ponds. The invention patent mainly includes: a lifting unit, a moving unit, a main frame, and a control unit. The lifting unit is responsible for raising and lowering the hook frame and automatically attaching and detaching the hooks; the moving unit provides power for the transfer machine to move on the seedling pond; the main frame supports and connects the various units of the transfer machine, keeping each unit in a relatively correct position and bearing various loads inside and outside the machine. During the transfer process, the control unit issues different commands to control the different units of the transfer machine to coordinate the entire work. This invention patent reduces labor intensity, decreases seedling mortality, and greatly improves the efficiency of the transfer process.

The invention discloses a sea cucumber feed as well as a preparation method and application thereof, and belongs to the technical field of sea cucumber feed preparation. According to the technical scheme, every 100 g of feed comprises 40 g of sea mud, 15-25 g of gulfweed powder, 5-15 g of enzymolysis gulfweed liquid and 1 g of multi-vitamin multi-mineral premix. The dosage of the enzymolysis gulfweed liquid is calculated according to the dry weight of the gulfweed, and the enzymolysis gulfweed liquid is obtained by performing enzymolysis on the gulfweed through algin degrading enzyme, pectinase and cellulase. The sea cucumber feed is applied to the aspect of sea cucumber feeding, solves the problem that the existing sea cucumber feed still cannot well promote accumulation of sea cucumber saponin and fucose in the body wall of the sea cucumber so as not to improve the nutritional value of the sea cucumber, and can effectively promote accumulation of sea cucumber saponin and fucose in the body wall of the sea cucumber so as to improve the nutritional value of the sea cucumber.

The utility model discloses a water quality regulation and control device for ecological culture of stichopus japonicus. The water quality regulation and control device comprises aeration equipment and a cleaning mechanism, the aeration equipment comprises an air compressor, a connecting pipe and an aerator, the aerator is connected with the air compressor through the connecting pipe, and a plurality of ventilation holes are formed in the aerator; the cleaning mechanism comprises a circular bottom plate and a cleaning scraping block used for cleaning impurities attached to the interior of the ventilation hole, the circular bottom plate is placed in the aerator, and the cleaning scraping block is arranged in the ventilation hole. Under the action of wind, an arc-shaped baffle is pushed to move up and down, under the connection of an arc-shaped push plate, a circular frame is driven to move up and down, meanwhile, a cleaning scraping block is driven to move up and down in a ventilation hole, impurities attached to the inner wall of the ventilation hole can be scraped and cleaned, and the situation that the ventilation effect of the aerator is affected due to the fact that the ventilation hole is blocked by the impurities in water is avoided.

The present invention provides a method for treating seawater aquaculture sediment as a feed source, a sea cucumber feed and a preparation method thereof, and belongs to the field of recycling waste resources in the aquaculture industry. The present invention dries and crushes the seawater aquaculture sediment, mixes it with stone powder, and places it in a microbial leaching device for elution to obtain deodorized seawater aquaculture sediment; the deodorized seawater aquaculture sediment, Bacillus subtilis, a nitrifying bacteria mixture and a special nutrient solution are mixed and fermented, and dried to obtain the feed source seawater aquaculture sediment. The present invention kills harmful bacteria in the sludge through a simple and efficient physical treatment method; designs a spray device to use water washing to reduce harmful heavy metals and salinity in the sludge; activates carbon, nitrogen and phosphorus in the sediment through enzymatic hydrolysis and fermentation; and realizes the feed source application of contaminated sediment by establishing a special sea cucumber feed with a sediment formula, thereby increasing the utilization value of the contaminated sediment, alleviating the pressure of the sea cucumber feed on sea mud, and promoting the sustainable development of the sea cucumber aquaculture industry.

The invention discloses a microbial premix product and application thereof in aquaculture, and belongs to the technical field of microorganisms, the microbial premix product comprises lysinibacillus and lactobacillus rhamnosus, the lysinibacillus is preserved in China Center for Type Microbiological Culture Collection, the preservation address is Wuhan University, Hubei Province, and the preservation number is CGMCC NO. The preservation number is CCTCC (China Center For Type Culture Collection) No.M20241816; the lactobacillus rhamnosus is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University, Hubei province, and the preservation number is CCTCC (China Center for Type Culture Collection) No.M20241815. The microbial premix product provided by the invention is helpful for promoting the growth of the stichopus japonicus, and can significantly reduce the cadmium content of the stichopus japonicus.

The invention relates to the technical field of stichopus japonicus culture, and discloses a multi-stage filtration and purification method and device for ecological culture of stichopus japonicus, and the method comprises the following steps: establishing two water purification channels, namely a rapid purification channel and a deep purification channel; collecting water body data of the culture pond, determining a hierarchical control strategy according to the water body data, and performing differential extraction on upper-layer water and lower-layer water; and purifying the water body according to the hierarchical control strategy, and enabling the purified water body to flow back to the culture pond. By establishing an up-and-down layered water pumping structure and a double-path purification system and combining intelligent layered judgment, double-path flow regulation and a synchronous maintenance mechanism, layered recognition, graded purification and dynamic balance control of multi-source pollution of the stichopus japonicus culture water are achieved, the bottom layer pollution removal capacity and the upper layer water circulation efficiency are improved, and the water quality of the stichopus japonicus culture water is improved. Long-term stable operation of the system is guaranteed, the water quality environment is effectively improved, and the breeding survival rate and ecological benefits are improved.

This invention relates to a land-based sea cucumber culture pond and a method for cultivating sea cucumbers. The land-based culture pond comprises: several culture units, each including a net reef and pond water near the reef for sea cucumber cultivation; and several heat exchange pipes, each including a third pipe laid near the culture unit and cold water within the third pipe. The cold water flowing through the heat exchange pipes lowers the temperature of the pond water near the net reef by 2-5°C. This invention solves the problem of how to regulate the bottom water temperature of the land-based sea cucumber culture pond, thereby improving the survival rate of sea cucumbers during summer dormancy.

This invention belongs to the field of marker-assisted breeding technology, and relates to the combination and application of SNP molecular markers for intestinal regeneration in *S. spinosaurus*. This invention uses the ratio of open intestinal length to total intestinal length during intestinal regeneration as a continuous trait indicator of intestinal regeneration capacity. Using whole-genome resequencing data and the BLINK model, genome-wide association analysis was performed to screen for 22 SNP molecular markers significantly associated with intestinal regeneration capacity in *S. spinosaurus*. The nucleotide sequences corresponding to these SNP molecular markers are shown in SEQ ID NO. 1-22, each sequence being 200 bp in length, with the SNP site located at the 100th bp. By detecting the genotype of these SNP sites, the intestinal regeneration capacity of *S. spinosaurus* can be evaluated at the molecular level, and superior individuals with faster intestinal regeneration can be screened for use in stress-resistant germplasm screening, marker-assisted breeding, and the construction of high-regeneration-capacity *S. spinosaurus* breeding populations.

The invention provides a kidney-tonifying and anti-fatigue polypeptide composition and a preparation method thereof, and belongs to the technical field of medicinal biological peptide complexes, the composition comprises oyster polypeptide, stichopus japonicus polypeptide, a blue ear grass-dilleniad fruit composite extract, a cistanche-dendrobium officinale composite extract, a ginseng extract and a penis et testis cervi extract; the oyster polypeptide is a common enzymolysis product of oyster meat through trypsin and bovine chymotrypsin; the stichopus japonicus polypeptide is a common enzyme product of enzymolysis of stichopus japonicus body walls by alkaline protease and collagenase type II and subtilisin; the blue ear grass-dilleniad fruit composite extract is a product obtained by carrying out enzymolysis on blue ear grass and dilleniad fruit, extracting with an ethanol water solution and purifying with an SA sulfonic group silica gel strong cation exchange column; the cistanche-dendrobium officinale composite extract is a product obtained by carrying out enzymolysis on cistanche and dendrobium officinale, extracting with an ethanol water solution and purifying with a phenylboronic acid affinity column-polyamide column tandem column; and the synergistic effect of tonifying kidney and resisting fatigue is realized.

The invention relates to the technical field of aquaculture, and provides a separated stichopus japonicus culture net cage device which comprises an outer frame, two sliding grooves are formed in the inner wall of the outer frame, a plurality of supporting frames are slidably connected to the interiors of the two sliding grooves, two clamping grooves are formed in one sides of the multiple supporting frames, and the two clamping grooves are connected with the two sliding grooves. Two clamping plates are fixedly installed on the other sides of the multiple supporting frames, the two clamping plates are movably embedded in two clamping grooves, a rotating rod is rotatably installed on one side of the outer frame, the outer surface of the rotating rod is movably sleeved with a blocking strip, a first threaded rod is rotatably connected to one side of the outer frame, and a second threaded rod is rotatably connected to the other side of the outer frame. In the embodiment of the invention, the net cage bodies are located in the multiple vertical grooves, the whole outer frame is placed in water, the stichopus japonicus is located in the net cage bodies for culture, and the multiple net cage bodies are distributed, so that the stichopus japonicus can be separated, and the situation that too much stichopus japonicus is accumulated in one net cage body is avoided; or the risk of disease transmission is caused.

The present invention relates to the field of sea cucumber aquaculture technology, and discloses a multi-stage filtration and purification method and device for sea cucumber ecological aquaculture, including: establishing two water purification channels, namely a rapid purification channel and a deep purification channel; collecting aquaculture pond water data, determining a stratified control strategy based on the water data, and performing differential extraction of upper and lower water layers; purifying the water body according to the stratified control strategy, and returning the purified water body to the aquaculture pond. The present invention establishes an upper and lower stratified pumping structure and a dual-path purification system, combined with intelligent stratified determination, dual-path flow regulation and synchronous maintenance mechanism, to achieve stratified identification, graded purification and dynamic balance control of multi-source pollution in sea cucumber aquaculture water bodies, thereby improving the bottom pollution removal capacity and upper water body circulation efficiency, ensuring long-term stable operation of the system, effectively improving the water quality environment, and increasing aquaculture survival rate and ecological benefits.

The present invention relates to a recognition model for wild sea cucumbers in seaweed beds based on stable isotopes and a method for constructing the same. The model of the present invention can identify a large number of sea cucumber samples through stable isotope detection and in combination with the parameter calculation method provided in this patent. The model and recognition method of the present invention can be applied to the direction of large-scale, rapid and accurate recognition of wild sea cucumbers in seaweed beds in the aquatic product market, are more easily promoted, and are conducive to the productized development of the wild sea cucumber market standardization.

The utility model relates to sea cucumber seedling breeding technical field, and disclose a kind of ecological aquaculture pond of sea cucumber seedling disease prevention and control, including aquaculture pond, and the prevention and control assembly of setting at the top of aquaculture pond, the prevention and control assembly includes the U-shaped frame slidingly connected in the top of aquaculture pond, the outer wall of U-shaped frame is fixedly connected with forward and reverse motor, the output of forward and reverse motor is fixedly connected with screw rod, the other end of screw rod is rotatably connected with the inner wall of U-shaped frame, the outer wall of screw rod is threadedly connected with sleeve block, the top of sleeve block is fixedly connected drive motor, the top outer wall of sleeve block is fixedly connected with annular frame, the output of drive motor is fixedly connected with driving shaft, the top outer wall of driving shaft is fixedly connected with driving gear, the side wall of driving gear is engaged with driven gear, by setting prevention and control assembly, it is convenient to drive pest lamp to drive away pest, to reach the prevention and control effect in the process of sea cucumber seedling cultivation.

The invention relates to an application of spectrum and light intensity in promoting increase of the number of thorns of stichopus japonicus, and belongs to the field of aquaculture. The application method comprises the following steps: culturing young stichopus japonicus under indoor culture conditions; the illumination period is set to be 12L: 12D circulation; in the culture process, blue light is adopted for irradiation, the wavelength of the blue light is 440-490 nm, and the illumination intensity is 1500 lx. According to the method, the technical effect of increasing the number of the thorns of the stichopus japonicus in the indoor environment is achieved by adopting the blue light irradiation with the specific wavelength and illumination intensity in the stichopus japonicus breeding process and combining the proper illumination period.

The stichopus japonicus culture pond comprises a pond body, step boxes are fixedly connected to the two sides of the outer wall of the pond body, the step boxes and the end of the pond body are jointly and fixedly connected with an equipment box, an electric push rod is fixedly connected to one side of the inner wall of the equipment box, and the telescopic end of the electric push rod is fixedly connected with a connecting block; the connecting block penetrates through the step box and is fixedly connected with a sealing plate, the two sides of the inner wall of the pool body are jointly and fixedly connected with a sliding groove matched with the sealing plate, a collecting drawer is arranged at the bottom of the pool body, a movable door corresponding to the collecting drawer is arranged on the side, away from the equipment box, of the pool body, and a circulating connector is arranged on the outer wall of the pool body. The sealing plate, the collecting drawer and the pond body are matched with one another, the bottom of the pond body is separated from a water area in the pond body through the sealing plate, the collecting drawer can be directly drawn out to be cleaned, the cleaning effect is better and more convenient, water in the pond body can be continuously and circularly purified, and normal cultivation of stichopus japonicus is guaranteed.

The invention relates to miRNA for preventing and treating chilo suppressalis and application of the miRNA in cultivation of insect-resistant rice. The invention verifies that overexpression of miRNA-989 in chilo suppressalis remarkably reduces vitellogenin deposition in oocytes, resulting in that the number of oocytes in nests is remarkably reduced, further miR-989 is integrated into a rice genome for overexpression based on an amiRNA technology, the resistance of miRNA-transferred rice to chilo suppressalis is verified, the safety and stability of miRNA-transferred rice are evaluated, and the miRNA-transferred rice has good application prospects. A new germplasm resource is provided for the prior art, and a new effective prevention and control strategy is provided for green prevention and control of agricultural pests.