64 results about "Raft culture" patented technology

A raft culture system capable of controlling a water layer consists of a boom rope, a balance floating ball string, an anchor rope, a fixed floating ball, a water layer regulation floating ball, a depth regulation rope, a balancing weight, a stake anchor and the like. The boom rope is provided with the balance floating ball string and the depth regulation rope; the water layer regulation floating ball is tied to the upper end of the depth regulation rope, and the balancing weight is tied to the lower end of the depth regulation rope; the culture water layer is controlled by controlling the length of the depth regulation rope; submergence of the boom rope is favorable for improving wind and wave resistance of the raft system in stormy weather and adapted to requirements of different cultured objects for the culture water layer. The system has simple machining process, convenient operation, long service life, is favorable for wide popularization and is adapted to the requirements for moving the raft facility culture offshore.

The invention discloses a fish and vegetable co-existing system which comprises a circulating water culture system, a biofilter, a water culture system, a water pump, a gas pump, a transmission pipeline and an electric control box. The circulating water culture system comprises a fish pond device and a whirlpool separator; the biofilter comprises an anaerobic mineralization pond, an aerobic mineralization pond and a moving bio-membrane reactor; the water culture system comprises a second low-land pond, a dry-wet medium bed, a first low-land pond and a plurality of deepwater floating raft culture beds; the fish pond device, the biofilter, the second low-land pond, the dry-wet medium bed, the first low-land pond and the deepwater floating raft culture beds are sequentially communicated; a water source of the first low-land pond is pumped out to enter the fish pond device to form a circulating system. The invention also discloses a use and control method of the system, so that two culture modes are mutually coordinated to operate in the same system, thereby reaching ecological balance and generating high economic and social benefits.

The invention relates to a method for preparing full-thickness skin used in toxicity testing by adopting stem cell raft culture, which comprises: 1. preparing and modifying a polymeric dermal scaffold; 2. preparing epidermal stem cells from embryonic stem cells and skin tissues; 3. preparing pancreatic islet cells; 4. preparing a special medium for the full-thickness skin; 5. and constructing the full-thickness skin. By utilizing the characteristics of strong differentiation and proliferation capacity of the epidermal stem cell, the skin morphology, organizational structure and functional activity of the constructed full-thickness skin of the invention can meet the demands of sub-chronic toxicity testing; the synthetic scaffold material has high degree of standardization and small batch-to-batch variation; as a serum free medium is adopted through the skin construction process, the factors influencing tissue construction are reduced, thus providing a foundation for the future use in toxicity testing. The all-thickness skin prepared by the method of the invention is closer to a natural skin, the application of which in the mode of toxicity testing and detection index agrees with practical situation better, thus being able to replace animals to be directly applied to the skin toxicity testing of chemicals, cosmetics, medicines and other health-related products.

A movable bottom raft culture system consists of a main rope, a floating ball string, an anchor rope, a culture cage, balancing weights, an iron anchor, a fixed floating ball and a mark floating ball which are interconnected. One end of the anchor rope is connected with the main rope, and the other end is connected with the iron anchor; the floating ball string is evenly tied to the main rope; two ends of the main rope are respectively provided with a balancing weight; the fixed floating ball and a fixed floating ball connection rope are arranged at proper positions of the anchor rope; the culture cage and the mark floating ball are also tied to the main rope. The system adopts a bottom culture mode, which is favorable for improving wind and wave resistance of the raft culture system in stormy weather and can develop a bottom culture sea area. The system has the advantages of convenient operation, integral movement and long service life, is convenient for wide popularization and is an ideal benthos culture device.

The invention discloses an integrated large-scale raft type culture device. The culture device comprises a body and a fixed berthing device for fixing the body, wherein the body comprises a PE float of which the top is provided with a working platform, and at least six cantilevers connected with the side of the PE float. The PE float has a cavity; the cantilevers are radially arranged along the side of the PE float; an I-shaped steel bar is connected with the bottom end of the PE float; and a weight is connected with a cable on the underside of the I-shaped steel bar. The culture device has the advantages that: the device has a lower integral center and good stability, and realizes the function of controlling the culture water level conveniently; in addition, the culture device has simple operation, great culture space and high production efficiency; and the operating personnel can walk and patrol on the device so as to conveniently find out hidden dangers and carry out the harvest operation. The integrated large-scale raft type culture device has a reasonable structure, good stability, high culture efficiency, convenient patrol and harvest, and simple water level control.

The invention discloses a combined elevation and raft type cultivation device which comprises a floating pipe connecting with floating balls, wherein cultivation cages arranged along the floating pipe are hung below the floating pipe through limiting ropes; a floating block is arranged at the top end of each net cage; and a sinker is arranged at the bottom of each net cage. When the combined elevation and raft type cultivation device is used in sea area, the floating pipe can effectively avoid the problems that the cultivation cages are extruded forwards and backwards, the cultivation space is reduced, and the cultivation cage bodies are turned over, which are caused by using the traditional rope approach; the combined elevation and raft type cultivation device allows the cultivation spaces of the cultivation cages to be kept constant and the spacial position state of the cultivation cages to be relatively stable, so as to avoid production decline caused by uneven distribution of cultivation cages due to forward and backward extrusion when shellfishes are cultivated; the combined elevation and raft type cultivation device has a large capacity and a stable aquaculture water space; and the combined elevation and raft type cultivation device has the advantages that the structure is firm, the spatial state is stable, the process is simple and the cost is low, has a certain resistance to wind and waves, and can realize manual adjustment of lighting in a natural sea area.

The invention discloses a near-shore aquaculture area remote sensing image extraction method based on multi-feature and spectrum fusion, and the method comprises the steps: firstly preprocessing an inputted original remote sensing image, extracting a feature spectrum through a feature index method, and forming a ground feature feature set; secondly, using an improved constraint energy minimizationalgorithm for enhancing a target ground object in a culture area and weakening background spectral information; then using an Otsu method for calculating a threshold value, and preliminarily extracting the target ground object in combination with a single-waveband threshold value; and finally, carrying out customized elimination on the preliminarily extracted result by applying a gray-level co-occurrence texture matrix or an object-oriented method according to the ground object characteristics, and outputting a final result of culture area extraction. Compared with a traditional target detection method, the method has the advantages that even in a breeding area with the obvious foreign matter same spectrum phenomenon, background ground object interference can still be effectively overcome, a high-precision raft breeding area extraction result can be obtained, and the high-precision extraction requirement of the raft breeding area can be well met.

The invention relates to a floating raft culture area extraction method based on land resource satellite image, which is characterized in that the method comprises the following steps: 1) on the basisof remote sensing image segmentation, extracting water body area in the remote sensing image by using NDWI index; 2) Based on the small-scale segmentation of water body region, the potential aquaculture region P1 is extracted by using the NDVI visual salient features; 3) calculate that marginal overlap degree of the plaques in the potential aquaculture area P1, further screen the plaques P1 to obtain a more accurate potential aquaculture area P2; 4) calculate that relative width of the plaques in the potential aquaculture area P2, further screen the P2 to obtain the final extraction result. On the basis of the NDVI visual salient feature, the method of the invention utilizes the salient feature of the edge of the aquaculture area, introduces the concept of edge overlap degree, and realizes the high-precision extraction of the raft aquaculture area in the land resource satellite image.

The invention discloses a floating raft type culture method of oysters. The floating raft type culture method comprises the following steps of selecting a culture site, fixing a culture floating raft, and stocking; selecting seed oysters with the size of 5 to 10mm, and enabling 20 to 30 seed oyster individuals to be attached to each attaching sheet; stocking according to the density of 100 to 110 attaching sheets in per square meter; when juvenile mollusks grow to 5 to 10mm, culturing the juvenile mollusks in a brackish water inner bay culture environment with abundant baits and the salinity of 0.9 to 1.5 percent until the juvenile mollusks grow to 50 to 55mm; after the juvenile mollusks grow to exceed 55mm, putting the juvenile mollusks in a brackish water environment with the abundant baits and the salinity of 2 to 2.5 percent for culturing; when the juvenile mollusks grow to 10cm or above, transferring the juvenile mollusks into a high-salinity environment with the abundant baits and the salinity of 2.5 to 3 percent, fattening for 30 to 90 days, and harvesting. According to the culture method of the oysters, the survival rate is high, the culture cost of the oysters is greatly reduced, and economic benefits are increased.

The invention provides a shallow sea raft culture method suitable for healthy and efficient growth of shellfish. The technical scheme is described as follows: the method comprises iron anchor fixing rings (1), floaters (2), lifting ropes (3), clamping lugs (4), cross rods (5), floater fixing rings (6), vertical rods (7), mooring ropes (8), iron anchors (9), culture cages (10), rope slings (11), valve rods (12), lower-layer valve rods (13) and upright rods (14). According to the method, raft culture is carried out from a near shore to a far shore and then at different sea areas of the near shore at different time periods, so that the cultured shellfish can be enabled to grow in an appropriate water environment all the time; by drawing up distribution points of a culture sea area, the water environment is monitored regularly, so that the quality and the yield of the shellfish can be ensured.

The invention relates to a cultivation method and apparatus for shadow sea shellfish dejection reutilization, wherein the shadow shellfishes and deposit-feeding animals are cultivated in a mixed manner in segregated regions of a common cage, the apparatus comprises a cage with a bottom disc arranged on the bottom portion, a cultivation chamber enclosed by web-shape articles is formed between the bottom disc and the cage, the commonly used shadow sea shellfish multi-layer cultivation cages can also be employed, each other layer of the cultivation cage is a fine-mesh structure. The method can alleviate the self-contamination in shallow sea shellfish culture.

The invention discloses an ocean tidal current energy power generation and shellfish culture composite system, and belongs to the field of culture equipment. The ocean tidal current energy power generation and shellfish culture composite system comprises a tidal current energy power generator foundation pillar, fixed rings, a turbine, a connecting rod, net frames, clamping buckles and a net body, wherein the two fixed rings are arranged on the tidal current energy power generator foundation pillar; the connecting rod is arranged between the fixed rings; the turbine is arranged on the connecting rod; the net frames are arranged on the fixed rings; the net body is arranged on the net frames. According to the system, equipment for anchoring and the like required in the conventional raft culture can be omitted, so that the overall wind power generation area simultaneously has the function of culturing shellfish, the labor and the equipment cost are reduced, and moreover, the large water flow in a tidal current energy area is more suitable for the rapid growth of the shellfish.

The invention discloses an offshore buoyant raft culture area extraction method based on SAR and optical image fusion. An optical image and SAR image fusion mode is adopted to extract a marine culturearea, a fusion algorithm most suitable for marine culture area extraction is obtained through experiments, and the precision of offshore water area buoyant raft culture area extraction is effectivelyimproved. According to the experimental result, the marine buoyant raft culture range can be accurately defined; the method is beneficial for government departments to more effectively supervise offshore culture areas, standardize the culture density of the culture areas, purify the culture water area environment, relieve the contradiction between the increase of transport ships and the continuous expansion of the culture water area, prevent commercial ships from entering the culture areas by mistake to pollute the culture water area, and ensure the sailing safety of the ships.

The invention belongs to the technical field of mariculture, and provides a raft culture rapid connection and disengagement device for solving the technical problems existing when existing seedling ropes and frame ropes are connected through hanging ropes.The raft culture rapid connection and disengagement device is composed of a slide frame and a stirrer.The stirrer comprises a stirring plate.A slide sleeve penetrating through the stirring plate is arranged in the middle of the stirring plate.The stirring plate and the slide sleeve are integrally formed in a crossed mode.Hollowed-out grooves are formed in sleeve walls of the two sides of the slide sleeve on one side of the stirring plate respectively and extend to the stirring plate from the bottom ends of the sleeve walls.The slide frame is located in the slide sleeve and is defined by a top frame, a bottom frame, a left frame and a right frame.The distance between the left frame and the right frame is consistent with the width in the slide sleeve.Telescopic contraction can be achieved between the left frame and the right frame.The left frame and the right frame extend to the position below the bottom frame.Anti-disengagement ratchets are arranged on the outer edges of the bottoms of the left frame and the right frame.The raft culture rapid connection and disengagement device is reasonable in structure, the connection and disengagement efficiency of seedling ropes can be greatly improved, and the raft culture rapid connection and disengagement device is particularly suitable for mechanical work and harvest.

The present invention utilizes AAV as a vector to transfect epithelial cells with an AAV/heterologous gene containing/Neo vector, to form a heterologous protein secreting culture of epithelial cells. This culture is useful for preparing recombinant skin using the organotypic epithelial raft culture system. The sheets of epithelial cells is composed of a stratified sqaumous epithelium composed of a lower layer of immature basal cells and an upper layer of mature keratinized epithelium. The rAAV virus stock prepared without wild type AAV is useful in expressing heterologous protein, however, the maximum production of the heterologous protein was achieved; when the rAAV virus stock contained wild type AAV. This invention discloses that AAV is appropriate for genetically altering skin to secrete new proteins to treat diseases and skin disorders or conditions.

The invention relates to a scallop cage allowing quick sewing and belongs to the technical field of fishery engineering equipment. The scallop cage comprises a scallop cage body, a sewing zipper and a single-page closing surface, wherein the scallop cage body is a soft cylinder defined by plastic netting, and a plurality of compartments for accommodating cultured objects are isolated in the middle by hard plastic isolation pieces with meshes. The sewing zipper is mounted at the opening of the scallop cage body. The single-page closing surface covers the sewing zipper and is divided into a fixed side and a free side, the fixed side of the single-page closing surface and one side of the sewing zipper are fixed together in a sewing or thermal bonding manner, and the free side of the single-page closing surface is combined with the other side of the sewing zipper through a Velcro tape and a magnetic-attracting concealed button. A traditional manual needle and thread sewing procedure for scallop cage production can be replaced, manpower and material resources are saved, the production efficiency and benefit of raft culture production seedling sub-packaging can be improved substantially, and the scallop cage has bright practical application and marketing prospect.

The invention discloses an offshore wind power generation and shellfish culture integrated system, and belongs to the field of culture equipment. The offshore wind power generation and shellfish culture integrated system comprises fixed rings, wind power generator foundation pillars, a net body, a hoister, a cable, buckles and a fence, wherein the net body is arranged in the four wind power generator foundation pillars through the fixed rings; the hoister is arranged on one wind power generator foundation pillar and is connected with the net body through the cable. According to the system, equipment for anchoring and the like required in the conventional raft culture can be omitted, so that the overall wind power generation area simultaneously has the function of culturing shellfish, and the labor and the equipment cost are reduced.

The invention discloses a raft culture bracket and a use method thereof. The raft culture bracket comprises sealed connecting terminals, transverse rods and vertical rods, wherein first holes matched with second holes in the sealed connecting terminals are formed in both ends of the transverse rods, the sealed connecting terminals are assembled on both ends of the transverse rods, the second holes in the sealed connecting terminals are matched with the first holes in the both ends of the transverse rods, the positions of the second holes are overlapped with those of the first holes to form vertical rod mounting passages, and one of the ends of the vertical rods is arranged in the vertical rod mounting passages, so that the vertical rods are vertically connected with the sealed connecting terminals and the transverse rods. The bracket is made of an anticorrosive material, and through the sealed combined structural design, the bracket keeps good sealing property and buoyancy, the corrosion of the seawater development environment is effectively avoided, the service life of the raft culture bracket is prolonged, and the breeding of bacteria that cause plant diseases and insect pests to aquatic plants in a culture process is effectively avoided at the same time.

The invention discloses a device and method for lifting seabed nutritive salt in a shellfish buoyant raft culture area. A shellfish buoyant raft comprises a buoyant raft and a limiting cable connected between the seabed and the buoyant raft. The device for lifting the seabed nutritive salt in the shellfish buoyant raft culture area comprises a bottom raft arranged on a seabed, a plurality of sets of injection pipes are fixedly arranged on the bottom raft, and a plurality of nozzles are formed in each set of injection pipes; and the injection pipes communicate with an air guide pipe, and the air guide pipe is connected with an upper pressurization mechanism and a lower pressurization mechanism. The upper pressurization mechanism and the lower pressurization mechanism are used for guiding air above the sea surface into the seabed to provide power for the air guide pipe, an air curtain is formed, upward flow is generated in the raft type culture area, bottom water with the high nutritive salt content is lifted to the surface layer for growth of bait microalgae, the abundance of the bait microalgae in the culture area is increased, the primary productivity of the raft area is improved, the survival rate and the yield of the raft culture shellfish are further improved, meanwhile, dissolved oxygen in the surface water body can be increased, and the effect of optimizing the seawater environment of the culture area is further achieved.

The invention relates to removal of fouling organisms on seaborne buoyant raft culture net cages, in particular to a method for automatically cleaning a net cage underwater. A bamboo pole penetrates through round holes in the centers of net cage separating plates in sequence, the net cage is straightened to the longest supporting state, the diameters of netting are basically identical, the net cage separating plates are on the same axis, the net cage is sleeved with a net scraper, locating rings are arranged on the upper and lower net cage separation plates so that the upper portion and the lower portion of the net scraper cannot be separated from the net cage, a sandbag is arranged on the lower portion of the net cage so that the time of the net cage drooping on the sea surface can be prolonged, the net cage is connected with a raft body through hanging ropes, and the upper portion of the raft body is connected with a buoyancy ball through a buoyancy rope. The net scraper can float to the top of the net cage without tides, the net scraper can move to the bottom of the net cage when the tides are increased to a certain degree, the net scraper can do high-frequency motion on the local portion of the net cage in the process through sea waves, and the movement has larger impact force so that the fouling organisms cannot be attached to the net cage to survive. The method has the advantage that the work efficiency of automatically cleaning the net cage underwater by the net scraper is improved.

The invention discloses a method for improving culture effects of oysters and rare seafood and application. According to the method, after the oysters subjected to raft culture grow to certain specifications, the oysters along with seed ropes are transferred to the sea floor of a proper sea area for relay bottom sowing culture, and rare seafood reefs are built by means of the oysters subjected tobottom sowing, so that excellent inhabiting spaces are provided for the rare seafood of sea cucumbers, sea urchins and the like. Through the method, a high-temperature crowded environment of a raft culture area can be effectively avoided, so that a growth rate and the fatness of the oysters are improved; and by conducting bottom sowing on the oysters, the inhabiting spaces can be provided for therare seafood of the sea cucumbers, the sea urchins and the like, feces and pseudofeces which are generated by the oysters can be used as excellent baits of the rare seafood, thus growth and survival of the rare seafood are facilitated, a sea area environment is restored at the same time, and the rare seafood feeds on fouling organisms on oyster shells, so that the labor intensity of shell cleaningafter the oysters are harvested is reduced, and the method for improving the culture effects of the oysters and the rare seafood and application has very wide application spaces in subtropical sea areas.

The invention relates to a mooring rope lifting and dragging device for raft type shellfish culture. The device comprises a stand column arranged on a side of a pontoon, a sliding seat sliding along the stand column and a winch fixed to the stand column; a rotatable groove face wheel is fixed to the sliding seat and protrudes out of the side, and a wheel body of the groove face wheel is parallel to the side; the groove face wheel is provided with a power source driving the groove face wheel to rotate; and a rope of the winch is fixedly connected with the sliding seat located below the rope. By using the device, traditional rope lifting and dragging work of fishermen during shellfish harvesting is replaced, main harvesting work is completed at a position above a deck, and physical output of workers is greatly reduced. Meanwhile, continuous operation can be achieved through mechanical work, the work time is greatly saved, and the work efficiency is improved. Besides, the device is used for seedling distribution and harvesting work of a long-mooring-rope shellfish culture raft frame, and the device can be applied to the working condition of single-mooring-rope lifting and dragging on the water surface.