25 results about "Epidermal stem cell" patented technology

The invention discloses an epidermal skin model and a construction method and application thereof. The construction method comprises the following steps: performing gas-liquid culture on a transwell membrane on a 3D epidermal organ prepared from epidermal stem cells to prepare an epidermal skin model. The epidermis skin model prepared by the construction method can be rapidly amplified to realize large-scale modeling application in a short time, and the epidermis skin model is complete in differentiation, high in integrity, good in barrier function and beneficial to epidermis-related safety detection or efficacy detection of drugs or cosmetics.

The invention belongs to the technical field of biological medicines, and particularly discloses an inflammatory memory activated epidermal stem cell bionic vesicle as well as a preparation method and application thereof. The epidermal stem cell bionic vesicle is obtained by adding lipopolysaccharide into a serum-free culture medium to induce and culture epidermal stem cells, continuously extruding a cell suspension through an extruder of a micron-sized polycarbonate film, and carrying out centrifugal separation. The LEM preparation method provided by the invention is simple, efficient and good in biocompatibility, not only retains the repair characteristic of mother cells, but also can induce neutrophil to transform to N2 phenotype, and has a better inflammation regulation effect, so that wound healing and tissue repair are promoted, and the LEM has a better application prospect and a better application value.

The invention provides application of maremastat in preparation of a medicine for promoting skin stretching and regeneration. The step of promoting skin stretching and regeneration comprises the substeps of treating or delaying skin stretching and regeneration capacity depletion; the step of promoting skin stretching regeneration comprises the substeps of promoting epidermal cell proliferation, increasing epidermal thickness, delaying damage of an epidermal adhesion structure and preventing epidermal stem cell identity loss; the invention provides the new application of the maremaxostat for the first time, namely the application of the maremaxostat in preparation of the medicine for promoting skin stretching and regeneration.

According to the application of the dipsacus asper saponin VI in regulating and controlling the differentiation of the surface stem cells, the brand new pharmacological action of the dipsacus asper saponin VI in targeted promotion of the differentiation of the epidermal stem cells is found for the first time, the cognitive limitation of the prior art is broken through, a brand new target spot and direction are provided for research and development of innovative drugs for wound repair, and the application of the dipsacus asper saponin VI in regulating and controlling the differentiation of the epidermal stem cells is realized. A medicine containing teasel saponin VI is adopted, epidermal stem cells are taken as a core target, wound reepithelialization is accelerated by promoting directional differentiation of the epidermal stem cells, the mechanism is clear, the targeting property is high, a core link capable of directly hitting wound healing is adopted, epidermal stem cell differentiation is regulated in a targeted manner, and meanwhile, the medicine has the synergistic effects of resisting inflammation and promoting angiogenesis; the obvious application potential is realized on refractory wounds such as diabetic feet and pressure sores.

The present disclosure relates to providing a substance and a composition that promote the proliferation of epidermal stem cells and are effective in improving wrinkles and barrier function from the viewpoint of maintaining or improving quality of life (QOL) in terms of both health and beauty. More specifically, a composition including 1-(2-hydroxyethyl)-2-imidazolidinone (HEI) or a derivative thereof and a seaweed extract such as Marinwort IPC-12 AGS can be used to promote the proliferation of epidermal stem cells and obtain a beneficial effect on wrinkles and barrier function.

The invention discloses a biological additive for promoting poultry feather development and application thereof, and belongs to the technical field of animal breeding. The biological additive is prepared from scallop skirt oligopeptide and stiff silkworm polypeptide according to the mass ratio of 1: 1. Animal experiments show that when 60 mg of the biological additive is added into every 1 kg of basic feed to feed broilers, the growth of the broilers can be promoted, and more importantly, the feather development of the broilers can be remarkably and synergistically promoted, and the length of main wing feather is obviously increased. The cytology research further proves that the biological additive can synergistically promote the proliferation of the poultry hair follicle epidermal stem cells, and the effect of the biological additive is superior to that of EGF positive control. Therefore, the biological additive developed by the invention is safe, efficient and green, effectively solves the problems of drug residue and drug resistance possibly caused by the traditional chemical additive, provides a sustainable solution for poultry breeding, and has important economic and application values.

The application discloses a biological additive for promoting poultry feather development and application thereof, and belongs to the technical field of animal breeding. The biological additive is prepared from scallop skirt oligopeptide and cantharides polypeptide at a mass ratio of 1:1. Animal experiments show that, when meat chickens are fed with 60 mg of the biological additive per 1 kg of basic feed, the growth of the meat chickens can be promoted, and more importantly, the feather development of the meat chickens can be significantly and synergistically promoted, and the length of the main wing feathers is obviously increased. Cytological studies further prove that the biological additive can synergistically promote the proliferation of poultry hair follicle epidermal stem cells, and the effect is better than that of an EGF positive control. Therefore, the biological additive developed by the application is safe, efficient and green, effectively solves the problems of drug residues and drug resistance possibly caused by traditional chemical additives, provides a sustainable solution for poultry breeding, and has important economic and application values.

The invention relates to the field of tissue engineering biological materials, in particular to an epidermal stem cell-loaded imitation skin ridge microneedle and a preparation method and application thereof, and the method comprises the following steps: providing a target skin sample; a three-dimensional structure of the target skin sample is obtained through laser femtosecond three-dimensional reconstruction, image processing software is adopted for analysis and quantification, and skin ridge size parameters of the target skin sample are obtained; preparing a microneedle mold according to the skin ridge size parameters; respectively preparing a needle tip solution and a substrate solution, and adding the epidermal stem cells in the culture logarithmic phase into the needle tip solution to obtain a mixed solution; filling a microneedle area of a microneedle mold with the mixed solution, and performing centrifugal treatment; and filling a substrate area of a microneedle mold with the substrate solution, carrying out illumination crosslinking, and demolding to obtain the epidermal stem cell-loaded artificial skin ridge microneedle. The method can realize stable survival of epidermal stem cells in the artificial skin ridge microneedle, and has the effects of reconstructing a skin wound skin ridge structure and improving wound healing quality.

The invention belongs to the technical field of biomedical materials, and particularly relates to an intelligent delivery patch based on epidermal stem cell adhesion regulation and a preparation method of the intelligent delivery patch. The intelligent delivery patch is prepared from the following raw materials in parts by weight: 1-5 parts of a drug-loaded compound, 8-15 parts of methacrylated gelatin, 0.5-2 parts of an enzyme response cross-linking agent, 0.3-1.5 parts of a force sensitive linking ligand, 0.1-1 part of a cell adhesion ligand and 0.05-0.2 part of LAP, drug-loaded nanoparticles of which the surfaces are modified with beta-cyclodextrin are anchored through a hydrogel network containing an azobenzene group, MMP sensitive peptide and RGD peptide, and the drug-loaded nanoparticles and the RGD peptide are combined to form the intelligent delivery patch. Efficient capture and enzyme response desorption of epidermal stem cells are achieved through synergism of MMP sensitive peptide and RGD peptide, a force sensitive release system is constructed through a supramolecular subject and object formed by beta-cyclodextrin and azobenzene, long-acting delivery is achieved by responding to mechanical stimulation while burst release of drugs is inhibited, and wound repair is promoted synergistically.

The invention belongs to the technical field of artificial skin preparation, and particularly relates to a system and method for preparing artificial skin through 3D printing, the system integrates key steps of cell extraction, amplification, artificial skin preparation and culture and the like, rapid amplification of skin cells and efficient construction of the artificial skin are achieved, the overall efficiency is remarkably improved, and the cost is reduced. The preparation period is shortened; the integrated square cabin system provided by the invention can move, so that the timeliness of cell extraction is ensured; besides, the prepared artificial skin with the net ridge structure has a certain thickness, the upper surface and the lower surface are flat, and the artificial skin has a wavy staggered three-dimensional structure, so that more epidermal stem cell dryness can be reserved, and the maintenance of epidermal stem cell marker protein is facilitated.

The invention relates to the field of biological medicine, and discloses a method for extracting stem cells in skin tissue, which comprises the following steps: treating the skin tissue by using a dissociation mixed solution of recombinant trypsin-normal saline-antibiotic, and separating an epidermal layer from other layers; the method comprises the following steps: respectively digesting an epidermal layer and other layers in different enzyme solutions to respectively obtain an epidermal stem cell suspension and an adipose-derived stem cell suspension, modifying the epidermal stem cell suspension, filling the adipose-derived stem cell suspension and the modified epidermal stem cell suspension into electrophoresis equipment, separating, respectively collecting, centrifuging, and culturing to obtain the adipose-derived stem cell. And respectively obtaining epidermal stem cells and adipose tissue-derived stem cells. According to the method, double-layer enzymolysis and electrophoretic separation are combined, so that the accuracy and cell activity of extracting epidermal stem cells and adipose-derived mesenchymal stem cells are ensured, the cells are not damaged, the separation efficiency is high, the purity is high, the cell phenotypic stability is ensured, the differentiation tendency in cell culture is reduced, and the method is used for subsequent clinical or experimental research.

The present disclosure relates to a composition effective for improving skin condition from the viewpoint of maintaining or improving quality of life (QOL) in both health and beauty. More specifically, the present disclosure relates to a composition containing 1-(2-hydroxyethyl)-2-imidazolinone or a derivative thereof and a picolinamide, which can be suitably used as a cosmetic or the like beauty composition effective for improving skin condition due to promotion of increase of epidermal stem cells, promotion of increase of keratinocytes.

The invention belongs to the technical field of stem cells and genetic engineering, and particularly relates to application of a ZFP42 gene / protein as a target spot in preparation of a product for promoting epidermal stem cell proliferation. Researches prove that the overexpression ZFP42 gene of the epidermal stem cells of the elderly individuals not only can obviously promote the cell proliferation, but also can enhance the self-renewal ability and improve the regeneration potential of the elderly individuals. The invention provides a method for enhancing regenerative potential / proliferation of epidermal stem cells by promoting ZFP42 expression. Compared with the prior art, the method for promoting cell proliferation based on the endogenous factor ZFP42 has higher stability and controllability, and an innovative strategy is provided for skin regenerative medicine and wound repair. The invention provides a method for promoting the proliferation of the epidermal stem cells and improving the regeneration potential of the epidermal stem cells, and the epidermal stem cells prepared by the method can be used for preparing wound repair materials or skin grafts, can be used for skin regeneration treatment and have good application value and prospect.

The invention provides a preparation method of prefabricated biological 3D printing artificial skin for skin and hair follicle regeneration. The prefabricated biological 3D printing artificial skin mainly comprises GelMA, HAMA and skin stem cells. The preparation method of the biological 3D printing artificial skin comprises the steps that a hydrogel solution containing 50 mg of GelMA and 5 mg of HAMA per milliliter is prepared firstly, then 107-108 skin stem cells are added, and the proportion of dermal stem cells to epidermal stem cells is 1: 2. And finally, mixing the cells with the hydrogel, transferring into an injector, refrigerating for 3 minutes, transferring into a biological 3D printer, and preparing artificial skins of different models according to preset printing parameters. The optimized hydrogel material disclosed by the invention has excellent printability, relatively low solubility and swelling rate and stable mechanical properties. The hydrogel material can be matched with the skin stem cells to promote the dryness of the skin stem cells to be improved, and skin repair and hair follicle regeneration are better facilitated. The biological 3D printing artificial skin prepared by the invention can be used for promoting skin and hair follicles in wounds immediately or after in-vitro culture.

The invention discloses a 3D skin model based on photocuring bio-printing and a construction method thereof, and the construction method comprises the following steps: A, printing bio-ink by adopting a 3D printing technology to obtain a corium layer; b, inoculating keratinocytes or epidermal stem cells to the surface of a corium layer, standing for a certain time, and then carrying out immersion culture; c, the dermis layer obtained after immersion culture is completed is added into a gas-liquid culture medium for gas-liquid culture, and the 3D skin model is obtained; wherein laminin is added into the gas-liquid culture medium in the step C. By adopting the construction scheme of combining 3D printing of the corium layer with artificial culture of the epidermal layer, the batch stability can be improved, the artificial culture period can be shortened, the cost can be reduced, and the quality of the model can be improved; the epidermal layer and the corium layer of the prepared 3D skin model are tightly connected, the epidermal layer is completely differentiated, and the 3D skin model can be widely applied to safety evaluation and efficacy evaluation of cosmetics or drugs.

The application provides a serum-free cell cryopreservation solution for epidermal stem cells and a preparation method and application thereof, and specifically belongs to the technical field of stem cell cryopreservation.The serum-free cell cryopreservation solution for epidermal stem cells comprises the following components in the following amounts: 0 g / L to 50 g / L raffinose, 3 g / L to 30 g / L dextran, 0% to 30% (volume percent) glycerol, 0% to 10% (volume percent) DMSO, 0% to 30% (volume percent) platelet lysate, and the balance of Ham's F-12; wherein the values of DMSO and glycerol are not both 0%. The serum-free cell cryopreservation solution can significantly improve the proliferation activity and colony formation ability of epidermal stem cells after resuscitation.

The application discloses an amplification culture medium and a culture method of human adult epidermal stem cells, and relates to the technical field of stem cell in-vitro culture. The culture medium comprises the following components: fibroblast growth factor, interleukin-6, insulin-like growth factor-1, epidermal growth factor, insulin, growth hormone, hydrocortisone, Y-27632, A83-01, Forsklin, PY60, CHIR99021, linoleic acid, linolenic acid, alanine, asparagine, glutamic acid, glycine, proline, serine, arginine, N-acetyl-L-cysteine, beta-mercaptoethanol and CaCl2. The culture medium can continuously and stably culture human adult epidermal stem cells for more than 20 generations, and a more stable long-term in-vitro amplification system of epidermal stem cells is established. After continuous subculture, the epidermal stem cells can maintain a high proliferation rate and multiple differentiation potential, and have the ability to construct multilayer skin.

The present invention relates to a composition for skin improvement, skin damage treatment, or maintenance of epidermal stemness, the composition comprising nidogen as an active ingredient. The composition comprising nidogen as an active ingredient according to an aspect exhibits effects on the epidermis and the dermis through basement membrane strengthening such as strengthening of binding between the basement membrane and the extracellular matrix so as to improve the function of the basement membrane, and proliferating of epidermal progenitor cells and maintaining of stemness of epidermal stem cells so as to improve keratin turnover and promote collagen production and binding, and thus the present invention can be utilized for skin improvement including skin barrier strengthening, suppression or prevention of hyperpigmentation, moisturization, skin elasticity, anti-aging, or tissue regeneration through wound healing.

The invention provides stem cell outer vesicle gel with high anti-inflammatory and anti-fibrosis activity and a preparation method of the stem cell outer vesicle gel. The stem cell outer vesicle gel comprises polymer gel, nano calcium oxide, nano silver and stem cell outer vesicles, wherein the nano calcium oxide, the nano silver and the stem cell outer vesicles are dispersed in the polymer gel; the polymer gel is a photo-initiated polymer gel of methacrylated polylysine (epsilon-PL-MA), carboxylated chitosan (CMCS) and itaconic acid. According to the stem cell outer vesicle gel with high anti-inflammatory and anti-fibrosis activity, polymerization gel is formed through photo-initiation of methacrylated polylysine (epsilon-PL-MA), carboxylated chitosan (CMCS) and itaconic acid, and nano calcium oxide, nano silver and epidermal stem cell outer vesicles are dispersed in the polymer gel; the extracellular stem cell vesicle gel with high anti-inflammatory and anti-fibrosis activity is coated on epidermis, can inhibit epidermal cell fibrosis, and has an anti-inflammatory effect.

The invention discloses an amplification culture medium and a culture method of human adult epidermal stem cells, and relates to the technical field of stem cell in-vitro culture. Comprising the following components: a fibroblast growth factor, an interleukin-6, an insulin-like growth factor-1, an epidermal growth factor, insulin, a growth hormone, hydrocortisone, Y-27632, A83-01, Forsklin, PY60, CHIR99021, linoleic acid, linolenic acid, alanine, asparagine, glutamic acid, glycine, proline, serine, arginine, N-acetyl-L-cysteine, beta-mercaptoethanol and CaCl2. The culture medium can be used for continuously and stably culturing human adult epidermal stem cells for more than 20 generations, and a more stable epidermal stem cell long-term in-vitro amplification system is established; the epidermal stem cells can maintain high proliferation rate and multiple differentiation potential after continuous passage, and have the ability to construct multilayer skin.

This invention relates to the field of biomedicine, and particularly to the application of blueberry anthocyanins in promoting the proliferation of epidermal stem cells and wound re-epithelialization. This invention applies blueberry anthocyanins to the proliferation of epidermal stem cells, revealing that blueberry anthocyanins can promote the proliferation and migration of isolated epidermal stem cells, overcoming the technical bottleneck of slow proliferation and easy aging of EpSCs in traditional culture, and providing a feasible in vitro expansion protocol for constructing the cell numbers required for large-area wound repair.

The invention discloses an induced differentiation method of sebaceous gland cells, which comprises the following specific steps: adding epidermal stem cells into an induction culture medium, and carrying out induced culture for 5-7 days to obtain the sebaceous gland cells, wherein the adopted induction culture medium is not added with insulin and specifically comprises the following components: 50% of a keratinocyte culture medium, 50% of a sebaceous gland cell culture medium, 1% of antibiotics, 20-40 ng / mL of EGF, 10-40 ng / mL of KGF and 5-20 ng / mL of rosiglitazone. Growth factors EGF and KGF and rosiglitazone are simultaneously added into the induction culture medium, insulin is completely not added, normal division and proliferation of cells are guaranteed, and sebaceous gland cells which are good in growth state and high in differentiation degree are obtained in a short culture period; compared with SZ95, the expression quantities of various sebaceous gland cell markers are similar.

The invention relates to the technical field of biological medicine, and discloses an active EDA-A1 loaded engineered small extracellular vesicle and a preparation method thereof, the preparation method comprises the following steps: step 1) overexpressing EDA-A1 protein on epidermal stem cells in a lentivirus infection mode, and screening to obtain stably transfected strain cells stably overexpressing EDA-A1; 2) performing multiplication culture on the stably transfected strain cells, and adding a Furin protease inhibitor in the culture process; 3) after the multiplication culture is completed, collecting cell culture supernatant and extracting small extracellular vesicles to obtain engineered EDA-A1 small extracellular vesicles carrying EDA-A1 active fragments; compared with the EDA-A1 recombinant protein, the engineered small extracellular vesicles prepared by the preparation method provided by the invention have the advantages of being easy to obtain, capable of exerting the functions of the EDA-A1 protein, stable in property, not easy to degrade and the like, and have a relatively great application prospect in clinical treatment of diseases related to ectoderm dysplasia in the future.

The present disclosure relates to an application of hydrogen molecules in the preparation of a product for promoting stem cell activation. Various hydrogen intervention means and generation means may be adopted when hydrogen molecules are applied, promoting the activation of autologous stem cells such as epidermal stem cells, basal stem cells, hair follicle stem cells, bone marrow-derived stem cells, mesenchymal stem cells, spermatocytes and neural stem cells. The related content may be applied to various fields such as wound healing, tissue / organ repair, tumor treatment, nervous system disease treatment, infertility treatment and aesthetic medicine.

The application provides a stem cell exosome gel with high anti-inflammatory and anti-fibrosis activity and a preparation method thereof, which comprises a polymer gel, nano calcium oxide, nano silver and stem cell exosomes dispersed in the polymer gel; and the polymer gel is a light-induced polymer gel of methacrylated polylysine (epsilon-PL-MA), carboxymethyl chitosan (CMCS) and itaconic acid. The stem cell exosome gel with high anti-inflammatory and anti-fibrosis activity is prepared by light-induced polymerization of methacrylated polylysine (epsilon-PL-MA), carboxymethyl chitosan (CMCS) and itaconic acid to form a polymer gel, and then nano calcium oxide, nano silver and epidermal stem cell exosomes are dispersed in the polymer gel. The stem cell exosome gel with high anti-inflammatory and anti-fibrosis activity is coated on the epidermis, can inhibit epidermal cell fibrosis, and has an anti-inflammatory effect.