58 results about "Basal cell" patented technology

Compositions useful for treatment of a wide range of skin disorders including: pre-cancerous lesions, keratotic lesions, superficial basal cell carcinomae; squamous cell carcinomae; malignant melanoma, and radiation-induced burns. In some embodiments the treatments comprise contacting human or other mammalian skin with a composition according to the disclosure. In other embodiments, administration of compositions provided is by injection. Some embodiments provide for preventive use of compositions provided towards preventing some forms of skin cancer and skin cancer-related disorders by repeated application to healthy-looking skin. Methods for providing the compositions are disclosed.

The present invention relates to compounds, small interfering RNAs and compositions and methods of inhibiting tumorigenesis using agents that inhibit the sonic hedgehog and GLI signaling pathway, including agents that inhibit GLI expression, synthesis and/or function. The present invention also relates to particular biomarkers that can be used in the diagnosis and prognosis of cancer. Methods of treating cancer, including glioblastomas, medulloblastomas, basal cell carcinomas, prostate cancer and small cell and non-small cell lung cancer are also provided using small organic compounds, siRNAs and blocking antibodies that inhibit or block the SHH/GLI pathway.

The invention provides a variety of reagents for use in the diagnosis and management of cancer, particularly breast cancer. cDNA microarray technology was used to identify genes whose expression profile across a large group of tumor samples correlates with that of cytokeratin 5 and cytokeratin 17, markers for basal cells of the normal mammary lactation gland. The invention demonstrates that tumors that express cytokeratin 5/6 and/or 17 have a poor prognosis relative to tumors overall. The invention provides basal marker genes and their expression products and uses of these genes for diagnosis of cancer and for identification of therapies for cancer. In particular, the invention provides basal marker genes including cadherin3, matrix metalloproteinase 14, and cadherin EGF LAG seven-pass G-type receptor 2. The invention provides antibodies to the polypeptides expressed by these genes and methods of use thereof.

Disclosed is an in vitro method of transdifferentiating an epidermal basal cell into a cell having one or more morphological, physiological and/or immunological features of a glial cell. Also disclosed are such transdifferentiated cells and cell cultures derived from them. A kit for converting, in vitro, epidermal basal cells into cells having one or more morphological, physiological and/or immunological features of a glial cell is also disclosed.

Disclosed is a method of transdifferentiating an epidermal basal cell into a cell having one or more morphological, physiological and/or immunological features of a neural progenitor, neuronal, or glial cell by culturing a proliferating epidermal basal cell population derived from the skin of a mammalian subject; exposing the epidermal basal cell(s) to an antagonist of bone morphogenetic protein (BMP), such as fetuin, noggin, chordin, gremlin, or follistatin; and growing the cell(s) in the presence of at least one antisense oligonucleotide comprising a segment of a human MSX1 gene and/or a segment of a human HES1 gene, or homologous non-human counterpart of either of these. Also disclosed is a transdifferentiated cell of epidermal origin and cell cultures derived therefrom. In addition, methods of using the inventive transdifferentiated cell(s) and cell cultures to identify a novel nerve growth factor or to screen a potential chemotherapeutic agent by detecting the presence or absence of an effect, in vitro, on a morphological, physiological and/or molecular biological property of the transdifferentiated cell(s) are described, as is a method of using the transdifferentiated cell(s) and cell cultures to screen a potential chemotherapeutic agent to treat a nervous system disorder of genetic origin. A kit useful for practicing the methods is disclosed

The invention relates to a composition containing umbilical cord mesenchymal stem cell exosomes. The composition comprises the following components in percentage by mass: 0.02-0.6% of the umbilical cord mesenchymal stem cell exosomes, 2.5-5.0% of an antioxidant, 1.5-2.5% of algal polysaccharides, 5.6-12.5% of a humectant, 0.05-0.6% of nicotinamide, 0.2-0.8% of hydroxyethyl cellulose, 0.1-0.5% of ceramide-2,, and the balance of deionized water. According to the composition, on the basis of adding the umbilical cord mesenchymal stem cell exosomes, the humectant, the antioxidant and the algal polysaccharides are matched, so that metabolism of skin cells can be accelerated, oxidation resistance and regeneration capacity of basal cells can be recovered, skin aging can be prevented, skin elasticity and plumpness can be increased, pores can be shrunk, and gloss can be improved.

The invention discloses a culture medium and culture method of a nasal mucosa tissue. According to the culture medium and culture method of the nasal mucosa tissue, epithelial-mesenchymal/matrix components are integrated in an organoid culture system based on a gas-liquid interface method for the first time, and by inducing proliferation and differentiation of adult stem cells in a fresh nasal mucosa epithelial tissue, an organoid which is composed of multiple kinds of cells, including ciliated cells, goblet cells, club cells and basal cells, and is close to an internal mucosa in structure andfunction can be obtained, and becomes an in-vitro nasal mucosa model with proliferation and multi-lineage differentiation capability.

A novel population of multipotent cardiac precursor (MCP) cells derived from human blastocysts derived stem cells is disclosed, methods for the preparation thereof and use of the cells for in vitro testing. Basement cells derived from hBS cells are also disclosed and method for the preparation of MCP cells from basement cells. The MCP cells have the following characteristics

• • i) at least 1% of the cells exhibit no antigen expression of one or more markers for undifferentiated cell, the marker being selected from the group consisting of SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 and Oct-4,
  • ii) at least 1% of the cells exhibit no protein expression of one or more of a neural marker including nestin or GFAP
  • iii) at least 1% of the cells exhibit protein and/or gene expression of one or more of a mesodermal marker including brachyury, vimentin or desmin
  • iv) at least 1% of the cells exhibit protein and/or gene expression of Flk-1 (KDR).

Furthermore, the MCP cells have a characteristic morphology. They grow as clusters of small, round and phase-bright cells; individual cells are 5-20 μm in diameter and each cluster is composed of 2-500 cells. They form clusters of round or elongated shape, that appear as loosely adherent cell clumps that as illustrated in FIG. 2 panel a, b and c. Furthermore, they have a relatively high nucleus-to-cytoplasma ratio, e.g. 1:2-1:64 of the total volume of the cell and/or appear as balloons on a string, as illustrated in FIG. 18, schematic sketch. Moreover, the MCP cells are non-contracting.

Compositions for inducing a natural tan of the skin is disclosed. The compositions contain D-ribose as the active ingredient plus a penetrant to deliver D-ribose to the basal cells and dermis of the skin. Compositions further containing emollients and emulsifiers are preferred.

The invention relates to a method for taking the breast cancer molecular marker S100A8/A9 in diagnosis and prognosis evaluation of the breast cancer. According to the method, results show that the expression indexes of the molecular marker S100A8/A9 in breast cancer patients with different molecular pathological subtypes are different, that is, thesubtypemolecular marker S100A8/A9 is highly expressed in a substrate cellular type (basal-like) breast cancer patient and a Her-2 amplified) overexpression type breast cancer patient, but is relatively low in expression index in a lumen epithelia A type (Luminal A) breast cancer patient and a lumen epithelia B type (Luminal B) subtype breast cancer patient, that is, the statistic difference is obvious. For published NKI breast cancer chip data, patients are divided into a high group and a low group based on S100A8/A9 for Kaplan-Meier survivorship curve analysis, results show that the negative correlation between the S100A9 expression level and the total survival rate is obvious, the prognosis of a breast cancer patient with high S100A9 expression is poor, and the prognosis application value of prompting breast cancer is high.

The invention provides a method for preparing a rabies vaccine stock solution from serum-free Vero cells. The method comprises the following steps: culturing Vero cells with a serum-free culture medium to obtain a serum-free culture medium adaptive cell line; establishing a serum-free Vero cell seed bank by utilizing the serum-free culture medium adaptive cell strain; recovering, culturing, performing passage and amplifying cells of the Vero cell working seed bank by using the serum-free culture medium to serve as basal cells for bioreactor culture, and continuously perfusing to culture high-density Vero cells with the serum-free culture medium by applying a bioreactor and a microcarrier after cell expansion; inoculating virus seeds of a rabies vaccine virus strain working seed bank, performing bioreactor microcarrier serum-free culture, starting to continuously perfuse to obtain a virus fluid when the virus is amplified to peak to obtain titer of the virus fluid; and performing clarifying, ultrafiltration concentration, inactivation and purification treatment to obtain the serum-free human rabies vaccine stock solution.

The invention discloses a dual-fluorescence staining solution for vaginal microorganism detection. The dual-fluorescence staining solution for the vaginal microorganism detection comprises an independent dual-fluorescence staining solution A and an independent dual-fluorescence staining solution B, wherein the dual-fluorescence staining solution A is composed of a fluorescence staining agent, an auxiliary staining agent, a staining solution buffer reagent, a bacteriostatic agent and water, and the dual-fluorescence staining solution B is composed of a staining solution buffer reagent, an alkaline regulating reagent, an anti-quenching agent, a bacteriostatic agent and water. The invention has the advantages of being simple in operation process, rapid in detection and easy to observe and recognize after pathogenic microorganism dyeing. Moreover, the interference of contaminated specimens such as bloody specimens, seminal fluid specimens and basal cell specimens on detection results is overcome, and the accuracy and the detection rate are effectively improved.

The invention discloses a gene group evaluating breast cancer molecule typing and a detection kit thereof. The gene group evaluating breast cancer molecule typing is a gene group composed of 216 genes. The invention also discloses the detection kit for the gene group evaluating breast cancer molecule typing. The detection kit comprises a primer capable of detecting the genes in the gene group for evaluating breast cancer molecule typing and cancer recurrence risk. An Illumina second-generation sequencing technology DASL-Seq is employed for detecting expression spectrum of cancer tissue, and on the above basis, breast cancer cases of Chinese women are divided into five molecule typing: 1) luminal type A, 2) luminal type B, 3) HER2 enrichment type, 4) basal cell type and 5) normal-cell like type, and propagation and immunization index are combined for calculating recurrence risk and pridicting recurrence possibility in 10 years, and thus individual treatment on breast cancer is guided.

The invention discloses a club fungus cultivating method and a product thereof. The club fungus cultivating method comprises the steps that basal cell division is promoted while auricularia polytricha sporocarp development is interrupted by adjusting and controlling temperature and light intensity and cooperating with proper high-concentration CO2, top end cells of auricularia polytricha bases are simulated to continue to divide and growth upwards when normal cell division in auricularia polytricha basal bodies is up to a certain degree, so that the auricularia polytricha bases undergo the expanding, growing and proliferating process and bifurcate from the base portions, the middle-upper portions bifurcate for multiple times to form clusters, the top ends form finger-shaped cluster sets, finally coral grape vine shapes are formed, and the appearance, taste and nutritive value of club fungi are superior to those of club fungi of auricularia polytricha.

The invention discloses facial beautifying apparatus. The facial beautifying apparatus comprises a shell, a steam nozzle, a massage head, a steam switch, a charging port and a battery. The facial beautifying apparatus integrates face steaming equipment and massaging equipment, wherein the massaging equipment adopts radio frequency (RF) wave and ceramic-magnetic-alloy polycrystalline composite wafers prepared by a nano-ultrafine technology. Acting on facial acupoints, the facial beautifying apparatus is capable of effectively penetrating deep skin at a depth up to 9 mm by strong penetration, activation and metabolism performance of bio-ion lightwave of the composite wafers, thereby causing high-frequency vibration at the acupoints so as to activate basal cells and cells in deep sleep; and moreover, water molecules are rapidly atomized by adopting high-frequency atomizing-vibrating, so that absorption is facilitated. In addition, face massage can be performed while carrying out of facialsteaming-beautifying, so that time and labor can be saved; and moreover, the facial beautifying apparatus has reduced space occupation size, and is convenient to carry.

Disclosed herein is a method for activating a dormant epithelial stem cell, or population thereof, to a state of multipotency comprising, reducing the level of ΔNp63 in the cell(s). The dormant epithelial stem cell(s) may be a horizontal basal cell (HBC) of the olfactory epithelium and the level of ΔNp63 may be reduced by contacting the cell or population with an effective amount of one or more agents that downmodulate ΔNp63. One example of an agent is an RNAi. Also disclosed is a method for treating olfactory dysfunction in a subject, comprising activating HBCs of the subject by reducing the level of ΔNp63 in one or more HBCs of the subject, to thereby treat the olfactory dysfunction. Activated horizontal basal cell (HBCs) are also disclosed.

The invention provides a noninvasive molecular subtyping kit and method for a breast cancer and aims to provide a kit which is simple to use and high in detection accuracy and a noninvasive molecularsubtyping method for a breast cancer, which makes samples easy to obtain, is simple in sampling process, good in clinical experience, capable of achieving processized operation and very suitable for clinical application and non-disease diagnosis purposes. The technical scheme of the invention is that the noninvasive molecular subtyping kit for the breast cancer comprises a plasma free DNA extraction reagent, a library construction reagent, a sequencing reagent and a sequencing chip. The method is based on high-throughput sequencing data of plasma free DNA, nucleosome footprint difference information of a promoter region in the whole genome range in the plasma free DNA is obtained by means of bioinformatic analysis, and thus noninvasive molecular subtyping of four categories of a lumen A type, a lumen B type, an HER2 enrichment type and basal cell type of a breast cancer patient is achieved according to the difference of footprint information. The invention belongs to the technical field of biology.

An application of the fritillaria alkaloid extract in preparing medicines for treating tumor, especially the basal cell cancer, is disclosed.

The present invention provides a basal cell culture medium and a feed medium with novel amino acid ratios and/or ferrous cholate as iron carrier that result in improved performance of mammalian cell culture processes, such as CHO cultivation and protein production processes, in particular in increased product titer (e.g. of monoclonal antibodies). Also provided are methods for culturing mammalian cells and producing a protein of interest using said basal cell culture medium and optionally feed medium. The invention also provides for a medium platform that comprises (i) the basal cell culture medium and (ii) the feed medium.

The present invention utilizes AAV as a vector to transfect epithelial cells with an AAV/heterologous gene containing/Neo vector, to form a heterologous protein secreting culture of epithelial cells. This culture is useful for preparing recombinant skin using the organotypic epithelial raft culture system. The sheets of epithelial cells is composed of a stratified sqaumous epithelium composed of a lower layer of immature basal cells and an upper layer of mature keratinized epithelium. The rAAV virus stock prepared without wild type AAV is useful in expressing heterologous protein, however, the maximum production of the heterologous protein was achieved; when the rAAV virus stock contained wild type AAV. This invention discloses that AAV is appropriate for genetically altering skin to secrete new proteins to treat diseases and skin disorders or conditions.

The invention discloses essence with wrinkle resisting and moisturizing functions as well as a preparation method thereof. The essence is characterized in that the essence comprises the following components in parts by weight: a component A: 8-12 parts of mulberry leaf essence, 10-12 parts of glycerol, 4-5 parts of alginate gel, and 100 parts of deionized water; a component B: 6-8 parts of towel gourd essence, 1.2-1.4 parts of glycerine monostearate, 2-4 parts of xanthan gum, 0.6-0.8 parts of sage essential oil, 0.6-0.8 parts of water soluble laurocapam, and 0.5-0.7 parts of panthenol; a component C: 3-5 parts of a sea bioactive substance, 0.3-0.5 parts of water-soluble vitamin E, 5-7 parts of squalene, and 3-5 parts of imidazolidinyl urea. Extraction efficiency of mulberry leaf essence reaches 85%, the mulberry leaf essence, the towel gourd essence, and the sea bioactive substance are combined with original creation, so that the essence does not damage skins, and has the efficacies for resisting wrinkle and moisturizing, resisting ageing, promoting proliferation of basal cells of skin, delaying aging of skin, promoting growth of cells, and activating skin cells; and the essence has good moisturizing effects, can effectively penetrate into skin, so that the skin is moisturized, tight and elastic.

The invention provides an ion introducing device based on a titanium membrane. The ion introducing device comprises an electrode part and an integrated circuit part, wherein the electrode part comprises a forward electrode and a backward electrode, and is used for exerting an electric field of which the polarities are opposite on the surface layer of skin of a human body to enable electrified ionsto migrate; the integrated circuit part is used for providing the electric field of which the polarities are opposite to the two electrodes; the forward electrode comprises a titanium membrane and aface membrane; one side of the face membrane is in contact with face skin, the other side of the face membrane is attached to the titanium membrane, and the face membrane is used for bearing an ionizable substance which is introduced into skin; the ionizable substance is ionized by the electric field formed by the electrode part, and then the ionized ionizable substance is introduced to the skin of the human body; and the titanium membrane comprises a base material and a metallic titanium layer attached to the base material. According to the ion introducing device disclosed by the invention, an extra-thin titanium membrane is adopted as a conducting material; a micro-current effect is produced, so that the absorbing of the functional ionizable substance by the skin basal cells is promotedto the maximum degree, the barrier function of the skin is repaired, and the skin state is improved.

The Claimed Invention is the topical-to-skin use of generic estradiol, estriol, conjugated estrogens, or any other synthetic estrogen within a non-absorbable generic vehicle of cream, lotion, ointment, shampoo, hair tonic, and wound-healing powder, having no absorbents or deconstructors in their formulas. This invention causes skin basal cells to produce increased quantities of collagen and to retain skin-water resulting in increased skin-thickness. This increased thickness results in improved resilience, resistance to injury, trauma and infection. The target of this invention is the skin and skin only. Specifically, there is no intended absorption of hormone into the circulation to result in side effects or risk. Any possible unintended and minimal quantity of hormone absorbed would be metabolized normally by the liver and excreted in the urine without risk or awareness to the user. Improving skin health and healing existing injuries/disease can save individuals from mid 30's and older, and society in general millions of dollars annually in skin-related health care costs.

The invention discloses freeze-drying powder for improving dry and sensitive skin and a preparation method thereof. The freeze-drying powder mainly contains milk immunoprotein, tetralin methyl pyrimidine carboxylic acid and cytothesis factors to inhibit inflammation signal factors such as IL-1, IL-6, IL-7 and TNF-a to balance the anti-inflammatory action, and scytitis of seborrheic dermatitis, contact dermatitis, eczema and the like can be effectively relieved; the releasing of histamine is inhibited to effectively relieve rubefaction and severe cutaneous pruritus; the cell lipid bilayer structure can be strengthened, the skin sebum barrier is reconstructed, the percutaneous water desorption of the skin is reduced, and the skin transdermal driage is normalized; the proliferation of skin cells can be rebalanced; the metabolism capacity of the cells is improved, the growth of basal cells is accelerated, the horn cell renewing is promoted, the separation of senescent and dying horn cellsform the skin tissue is accelerated, and therefore the pachulosis state is improved, and the surface of the skin is fine and smooth.

The invention belongs to the technical field of cosmetics, and specifically relates to an eye mask and a production technology thereof. The eye mask includes the following raw materials: rhodiola root, gotu kola, ginseng, Angelicae Sinensis Radix, Ligusticum wallichii, white poria, apricot kernel, Ampelopsis japonica, white peony root, Atractylodis Macrocephalae Rhizoma, Radix Astragali, Chinese yam, wrinkled giant hyssop, rose, honeysuckle flower, mint, borneol, edible alcohol, pearl powder, aloe powder, enzymatic collagen, Vitamin B, glycine, Vc-Na, hyaluronic acid, glycerine for medical purposes and Germall. Through the use of rhodiola root and gotu kola supplemented with eleven Chinese medicinal herbs, the eye mask can help the skin resist oxidation, inhibit free radical activity, inhibit tyrosinase activity, promote collagen regeneration of the skin, enhance cell activity of basal cells, maintain elastic and compact skin, regulate skin immunity and reduce melanin pigmentation, andalso can stimulate turnover of skin deep cells to make the skin become smoother and more elastic.