35 results about "Tretinoin" patented technology

Provided in the present invention is an analysis and detection method for simultaneously detecting tretinoin and tranexamic acid, and the use of the analysis and detection method. The method comprises: analyzing a sample to be tested comprising tretinoin and tranexamic acid by using an ultra-high performance liquid chromatography-triple quadrupole mass spectrum, so as to achieve the qualitative and quantitative detection of tretinoin and tranexamic acid. The analysis and detection method provided in the present invention has the characteristics of a high sensitivity and high precision, good repeatability, high accuracy, high stability, etc. The detection method of the present invention can be relatively easily transferred and applied to the detection of the two compounds in biological samples such as plasma and tissue homogenates, and can satisfy the requirements for drug concentration monitoring.

The invention provides an analysis and detection method for simultaneously detecting tretinoin and tranexamic acid and application thereof, and the method comprises the following steps: analyzing a to-be-detected sample containing tretinoin and tranexamic acid by using ultra-high performance liquid chromatography-triple quadrupole mass spectrometry to realize qualitative and quantitative detection of tretinoin and tranexamic acid. The analysis and detection method provided by the invention has the characteristics of high sensitivity and precision, good repeatability, high accuracy, strong stability and the like. The detection method disclosed by the invention can be easily transferred and applied to detection of two compounds in biological samples such as plasma and tissue homogenate, and can meet the requirement on drug concentration monitoring.

The invention discloses whitening essence and a preparation method thereof, and belongs to the field of skin care products. The five-phase mixture comprises hydroquinone, tretinoin, fluocinonide, glabridin and vitamin C derivatives; the phase A is prepared from water, PCA sodium, butanediol, sodium hyaluronate and 1, 2-hexanediol; the phase B is prepared from ascorbyl glucoside, arginine, a skin conditioner, chlorphenesin, hydroxyethyl piperazine ethanesulfonic acid, alpha-arbutin, nicotinamide, dipotassium glycyrrhizinate and a skin conditioning and relieving agent; a phase C: 1, 3-propylene glycol and a preservative; a phase D: PEG-40 hydrogenated castor oil and rose oil; and a phase E: water, 1, 2-pentanediol, a peppermint leaf extract and glutathione. Compared with the existing whitening essence in the market, the whitening essence prepared by the invention can be applied to different skin types, and can be absorbed by different skin types when acting on different skin types, so that the whitening essence prepared by the invention can be used for whitening different skin types when being applied to users with different skin types, and the whitening essence can be used for whitening different skin types. The whitening and moisturizing effects are good.

The invention discloses fluocinolone hydroquinone tretinoin acid cream and a preparation method of the fluocinolone hydroquinone tretinoin acid cream. The invention provides a preparation method of fluocinonehydroquinone tretinoin emulsifiable paste, which comprises the following steps: S02, preparing an oil phase: mixing fatty glyceride, a fatty alcohol mixture, C12-C20 alkanoic acid and polyoxyethylene (35) castor oil, adding tretinoin, and then adding liquid alkane; s031, at the temperature of 60 DEG C to 80 DEG C, a pH regulator, polyhydric alcohol and the magnesium aluminum silicate gel are mixed; mixing with the oil phase in S02, stirring and emulsifying; and S032, mixing the product obtained in the step S031 with an antioxidant, fluocinonide and hydroquinone, stirring, and emulsifying. By improving the existing preparation method, the tretinoin can be effectively prevented from crystallizing, and the content uniformity of the tretinoin can be improved.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to fluocinolone hydroquinone tretinoin acid emulsifiable paste and a preparation method thereof.The fluocinolone hydroquinone tretinoin acid emulsifiable paste is prepared from fluocinolone, hydroquinone, tretinoin, a matrix, a preservative, a humectant, an antioxidant, a thickener and an emulsifier; wherein the humectant is a mixed humectant of glycerol and methyl glucitol polyether-10 in a mass ratio of (1: 1.5)-(1: 3), and the quality of the cream product is favorably improved. In addition, by limiting the feeding sequence and the feeding temperature of the raw materials and the auxiliary materials in the preparation process, the fluocinolone hydroquinone tretinoin acid cream which is good in product quality and stable in release can be obtained.

Disclosed are a composition and a formulation. The composition comprises tranexamic acid and a tretinoin alcohol amine or tretinoin fatty amine compound. After the composition is prepared into the formulation, the solubility of tretinoin can be improved, the loss of tretinoin in the formulation process is reduced, the content uniformity of the formulation is improved, and the stability of the formulation is improved. The composition can significantly increase the intradermal retention amount of tretinoin and tranexamic acid, and meanwhile, the cross-skin permeation amount of tranexamic acid is not significantly increased. In the treatment of pigmentation and chloasma, the composition and the formulation can significantly improve the curative effect and avoid aggravating toxic and side effects.

The invention provides efficient mild whitening cream and a preparation method thereof. The raw materials of the cream comprise five phases A and E, the phase C contains 0.1-3% of a mixed solution of isosorbide dimethyl ether and hydroxyl pinacolone retinoate, and the phase D contains 0.01-0.1% of L-carnitine. Wherein the hydroxyl pinacolone retinoate can be specifically combined with an RAR / RXR (Region Receptor / Receptor X Receptor), and the expression of an MITF (Microphthalmia-associated Transcription Factor) gene is reduced, so that the melanogenesis is inhibited; l-carnitine promotes fatty acid beta-oxidation and enhances ATP synthesis to indirectly regulate and control AMPK activity; aMPK enhances transcriptional activity of a tretinoin receptor through phosphorylation of the tretinoin receptor, so that the AMPK and hydroxyl pinacolone retinoate exert a synergistic effect, jointly enhance whitening activity, effectively improve the phenomena of color spots and dark skin and promote the skin color to be uniform, bright and white; meanwhile, the isosorbide dimethyl ether can promote the transdermal absorption of the hydroxyl pinacolone retinoate, the L-carnitine and the related active ingredients.

The present invention provides a method for preparing tretinoin. The Wittig reaction product of [3-methyl-5-(2,6,6-trimethylcyclohexen-1-yl)-2,4-pentadienyl]-triphenylphosphonium salt (Compound of Formula II) and 5-hydroxy-4-methyl-2-furanone (Compound of Formula III), namely the mixture of C11,13-dicis-tretinoin (Compound of Formula IV) and isotretinoin (Compound of Formula V), is subjected to two-step isomerization reactions: copper-catalyzed isomerization reaction (a) and iodine-catalyzed isomerization reaction (b) to prepare tretinoin by a one-pot method. This preparation method can avoid heavy metal pollution, has high selectivity, low cost, and the raw materials are easy to obtain or prepare, and is suitable for the industrial production of tretinoin.

The present application belongs to the field of biomedicine, and relates to an inducer for inducing mesenchymal stem cells to differentiate into estradiol-secreting cells. The inducer for inducing mesenchymal stem cells to differentiate into estradiol-secreting cells is based on a human mesenchymal stem cell serum-free culture medium, and is composed of the following components in a mass concentration ratio: bone morphogenetic protein-4 20-60 mg / L, bone morphogenetic protein-7 20-60 mg / L, tretinoin 2-8 mg / L, resveratrol 2-8 mg / L, icariin 2-8 mg / L, benzamide 2-8 ug / L, chloroplatinic acid hexahydrate 2-8 ug / L, ethanolamine 2-8 ug / L, erythropoietin 2-10 ug / L, and vascular endothelial growth factor 2-10 ug / L. The inducer for inducing mesenchymal stem cells to differentiate into estradiol-secreting cells has high induction efficiency.

The invention provides compositions and methods for providing a cardioprotective effect in a subject. Specifically, the invention provides compositions and methods for treating cardiovascular ailments, for example, ischemia / reperfusion (I / R) injury, cardiac arrhythmias, oxidative stress, or cardiac failure by administering a retinoic acid receptor-beta (RARβ) agonist.

The present invention provides a tretinoin and benzoyl peroxide composition, its preparation method and use, which comprises component (i) benzoyl peroxide; component (ii) tretinoin; and (iii) a lipid material. The composition has excellent stability, and the preparation process is simple and easy to scale up production.

The present invention relates to a retinoic acid-alcohol amine complex consisting of retinoic acid and an alcohol amine compound. The complex is in liquid form at room temperature, has good water solubility / water dilution resistance and good skin penetration effect, and overcomes the limitation of retinoic acid compounds in the application field of drug products in the prior art, showing a wide prospect in medicine.

The invention belongs to the technical field of fluorescent probes, and particularly discloses a novel fluorescent compound capable of being combined with a retinoic acid receptor related orphan receptor gamma normal site and application of the novel fluorescent compound serving as a fluorescent probe to inhibitor screening, imaging and the like. Compounds P1-P11 conforming to the general formula (I) are synthesized, a novel ROR gamma normal inhibitor screening platform can be established on the basis of a fluorescence polarization probe system constructed by the compounds according to a competitive ligand replacement principle, and the technical scheme breaks through the limitation of a traditional activity detection method and has a wide application prospect. An activity evaluation tool with economical efficiency, timeliness and reliability is provided for research and development of drugs targeting ROR gamma normal sites, and the lead compound discovery and structure optimization process can be accelerated.

The invention belongs to the technical field of biological medicines, and particularly relates to preparation and application of a nanoemulsion. The PDA / TEMPO NMsE nano-emulsion prepared by the invention can block lipid peroxidation chain reaction of a skin photoaging part and effectively reduce the inflammatory mediator level, and compared with retinoic acid or polydopamine, the PDA / TEMPO NMsE nano-emulsion has a more excellent skin photoaging treatment effect, can keep a protection effect for a long time, and has a good application prospect. The problem that the efficiency of removing alkoxy and alkyl peroxy free radicals by a single PDA material is insufficient is solved.

The invention relates to the technical field of medicines, and discloses a refining method of tretinoin, which comprises the following steps: S1, dissolving a tretinoin crude product with a mixed solvent of dichloromethane and lower alcohol, adding activated carbon for decoloration, and filtering; and S2, distilling the filtrate obtained in the step S1, adding low alcohol in the distillation process for band removal, then cooling and crystallizing, washing crystals with low alcohol, filtering, and drying to obtain refined tretinoin. According to the refining method, the influence of impurities in the crude product can be avoided, a good technical effect is achieved, the purity of tretinoin reaches 99.9% or above, the single impurity content is not higher than 0.05%, the total impurity content is lower than 0.1%, the refining yield reaches 90% or above, and the refining method is suitable for industrial production.

Provided is a composition for improving wrinkles including a high-content / sustained-release retinoid capsule. The high-content / sustained-release retinoid capsule according to the presently claimed subject matter can minimize skin side effects and maximize wrinkle improvement effects by significantly increasing the retinoid content in the capsule and stability and controlling the release rate.

The application discloses a kind of vitamin A derivative tablets, comprising: 11% to 12% of tretinoin, 83% to 87.8% of the compound of two microcrystalline celluloses, 1.0% to 2.0% of magnesium stearate by weight percentage;Microcrystalline cellulose is selected from TF series, TL series;Two microcrystalline celluloses are TF525 and TL565 respectively;The weight ratio of two microcrystalline celluloses is 32 to 39:39 to 45.The preparation method of tablet comprises:1) weighing: the raw and auxiliary materials are weighed according to the prescription amount, and passed through 40 mesh screen;2) total mixing: the material is added into wet granulator, the stirring speed is set to 220-280rpm, the shearing speed is set to 1000-1600rpm, and the mixing time is 5-10min;3) tabletting: the theoretical tablet weight is 180±13mg, and the hardness is controlled to 30-50N;4) coating: the coating weight gain is 3-5%;5) aluminum package: the heat sealing temperature is set to 110-140℃, and the main machine speed is 4-8rpm.The tretinoin tablet prepared in the application has good dissolution performance and stability.

The invention discloses a 9 cis-retinoic acid nano-liposome and a preparation method thereof. The preparation method comprises the following steps: under a dark condition, dissolving 9 cis-retinoic acid, hydrogenated soybean phosphatidylcholine, DSPEPEG2000 and cholesterol according to a specific weight part ratio in an organic solvent, carrying out reduced pressure rotary evaporation to form a lipid film, adding pure water for hydration, and finally controlling the ultrasonic power and time or the high-pressure homogenization pressure and cycle index to complete particle size control. Through the synergistic effect of the prescription and the process parameters, the nano-liposome with the encapsulation efficiency greater than 95% and good stability is successfully prepared, and the problems of low encapsulation efficiency and poor stability are effectively solved.

The present invention relates to the field of applied basic medical research, specifically to a method for inducing a zebrafish neural tube defect model using all-trans retinoic acid (ATRA). This invention establishes, for the first time, a zebrafish model of neural tube defects induced by all-trans retinoic acid (ATRA), providing a new zebrafish model for studying the mechanisms and treatments of neural tube defects caused by early pregnancy ATRA. The technical problem addressed by this invention is to establish a convenient, time-saving method that allows for direct in vitro observation of the embryonic neural tube development process. This novel method for inducing a zebrafish neural tube defect model has a high embryonic malformation rate and a low mortality rate.

The present invention relates to a cosmetic composition containing a high concentration of retinoid to improve wrinkled skin, and more specifically, relates to a cholesteric liquid crystal cosmetic composition containing retinol, the composition having an excellent wrinkle-improving effect by containing a high concentration of retinoid while also being significantly less irritating to the skin.

The present invention relates to the use of a stable aqueous silicic acid solution in the preparation of a product for treating acne, wherein a mass concentration of the stable aqueous silicic acid solution is 0.5-2%. A product using the stable aqueous silicic acid solution as an active ingredient can be used for treating acne or for preventing and / or treating sebaceous gland dyshomeostasis. At a mass concentration of 0.5-2%, the product can lighten the color of sebaceous gland plaques, thereby achieving an anti-acne effect. Moreover, the stable aqueous silicic acid solution has a low raw material cost, is environmentally friendly and safe, and can thus be used as a highly therapeutically effective alternative to traditional tretinoin drugs.

The invention relates to compositions comprising urolithins with active agents, particularly compositions comprising urolithins and one or more retinoid compounds, for example, topical compositions of urolithins with one or more retinoids. The invention also relates to uses of such compositions, for use for the treatment or prevention of diseases, disorders and conditions, particularly diseases, disorder or conditions of the skin and hair, and for use in non-therapeutic treatments. The invention also related to a urolithin, for example, urolithin A, for use in reducing adverse effects of the administration of a retinoid compound.

The invention discloses a composition and a preparation method of fluocinolone hydroquinone tretinoin acid emulsifiable paste, and belongs to the field of pharmaceutical preparations. The fluocinolone hydroquinone tretinoin acid cream disclosed by the invention is simple in composition, stable in quality, simple, convenient and efficient in preparation process. Aiming at the problems that active ingredients of the fluocinolone hydroquinone tretinoin cream are poor in content uniformity and easy to crystallize and separate out, the low-content active ingredients fluocinolone and tretinoin are uniformly dispersed in a carrier material by using a melting adsorption granulation technology, and the rate of recovering a dispersed substance to a crystalline state is reduced by using the amorphous and adsorption characteristics of the carrier material; the surface free energy of the dispersed substance is reduced, the active component forms microcrystals or crystal grains are separated out, and the safety and the quality stability of the preparation are improved. Common equipment is used in the preparation process, industrial production can be achieved, and the preparation process is worthy of application and popularization.

The invention discloses a method for inducing human adipose-derived stem cells to be differentiated into insulin-secreting cells. The method comprises the following steps: separating and extracting human adipose-derived stem cells from human adipose tissues and enabling the human adipose-derived stem cells to be self-polymerized; placing the auto-aggregated and balled human adipose-derived stem cells in an induced differentiation medium for first-stage induction to obtain a first induced differentiation system; retinoic acid and a transformation factor beta1 are supplemented into the first induced differentiation system, and meanwhile, synergistic induction is performed in combination with near-infrared light irradiation to obtain a second induced differentiation system; and supplementing taurine, beta cytokine, insulin-like growth factors, nicotinamide and a BMPI type receptor kinase inhibitor into the second induced differentiation system, and carrying out synergistic induction through near-infrared light irradiation to obtain the insulin secreting cells. According to the method, the cell differentiation efficiency is remarkably improved, and a new theoretical basis is provided for differentiation from the human adipose-derived stem cells to the insulin secreting cells and application of the human adipose-derived stem cells.

The invention belongs to the technical field of pharmaceutical preparations, and relates to a composition for preparing a tretinoin soft capsule, the composition is prepared by mixing tretinoin, soybean oil and beeswax according to a certain proportion and then preparing a soft capsule content, the content can uniformly mix the tretinoin in an oily matrix, and the tretinoin soft capsule has better stability and redispersibility, so that the soft capsule can be used for preparing soft capsules. The method can be used for preparing tretinoin soft capsules, does not cause medicine sedimentation, and is stable and controllable in quality. The tretinoin soft capsule preparation prepared by the invention is suitable for clinical application, and lays a foundation for developing the tretinoin soft capsule which can be orally taken by children.

The present invention relates to a composition containing tretinoin, its preparation method, and its use. The composition comprises (a) a tretinoin-alcoholamine compound complex, (b) a gel matrix, and (c) an oil phase component. The composition has good ingredient compatibility, comfort in use, stability, safety, and skin permeability, and therefore has broad medicinal prospects.

The invention provides a method for detecting tretinoin and / or related substances thereof in a tretinoin preparation, and relates to the technical field of medicine detection. The method comprises the following steps: pre-treating a tretinoin preparation to be detected: mixing the preparation to be detected, a gel breaker and a diluent, carrying out solid-liquid separation operation, and collecting a liquid component which is a sample liquid to be detected; by adopting the pretreatment method, matrix interference in the preparation to be detected can be effectively removed. The to-be-detected sample solution is detected by high performance liquid chromatography, and rapid qualitative and quantitative analysis of tretinoin and seven related substances in the tretinoin preparation can be realized. Wherein the tretinoin and the seven related substances have good peak shapes, the separation degree of each substance is high, the accuracy of a detection result is high, the detection limit is low, the quantitation limit is low, the detection sensitivity is high, and the stability is strong. According to the detection method provided by the invention, the defect of detection of tretinoin and related substances in the tretinoin preparation in the prior art is overcome, and a technical support is provided for inspection and quality control of the tretinoin preparation.

The present disclosure provides methods for preparing tretinoin. The present disclosure also provides novel intermediates in the methods described herein. Also provided are novel polymorphs of tretinoin.

The application provides a separation method and detection method for erythromycin, isotretinoin and BHT in isotretinoin erythromycin gel, and the separation method comprises: the detection method adopts high performance liquid chromatography to separate erythromycin, isotretinoin and BHT in isotretinoin erythromycin gel; and the high performance liquid chromatography conditions comprise that a Shimadzu shim-pack Scepter C18-120 column is used as a chromatographic column; a mixed mobile phase of mobile phase A and mobile phase B is used for gradient elution; the content of erythromycin, BHT and isotretinoin can be determined at the same time, which is convenient, fast and practical.

This specification provides compositions and methods for treating skin disorders. In particular, this specification provides compositions that enhance retinoid-based skin treatment while preventing side effects.