137 results about "Glabridin" patented technology

The invention relates to the technical field of gel materials, and discloses whitening gel based on biological polysaccharide and a preparation method thereof, the whitening gel comprises the following raw materials: hyaluronic acid, deionized water and thunberg fritillary bulb polysaccharide microcapsules; wherein the bulbus fritillariae thunbergii polysaccharide microcapsule is prepared by taking konjac glucomannan, chitosan and perillaldehyde modified hyaluronic acid as a shell layer and embedding bulbus fritillariae thunbergii polysaccharide and glabridin as a core material; the perillaldehyde modified hyaluronic acid is prepared by reacting alkenyl hyaluronic acid with perillaldehyde under the action of an initiator; the alkenyl hyaluronic acid is prepared by reacting hyaluronic acid with glycidyl methacrylate under the action of a catalyst. The prepared whitening gel has excellent whitening, moisturizing, anti-oxidation and anti-aging effects, effective components are not prone to loss, and the whitening gel can be applied to the skin surface for a long time, plays a whitening role and delays the aging process of the skin.

The invention relates to the field of biological engineering and technology, in particular to an enzyme for synthesizing glabridin, a biological synthesis method of glabridin and a bacterial strain. The invention discloses a series of glabridin synthesizing enzymes (phenylalanine ammonialyase, cinnamic acid-4-hydrogenase, tyrosine ammonialyase, p-coumaric acid coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4 '-oxymethyltransferase, 4'-hydroxymethyltransferase and 4 '-hydroxymethyltransferase) derived from glycyrrhiza glabra for the first time. ) can be used in the preparation of a compound (e.g., isoflavone 2 '-hydrogenase, isoflavone reductase, Vissisoketoreductase, isoprenyltransferase, pterocarpin reductase, oxidative cyclase, demethylase, cytochrome P450 reductase, and the like). The de novo synthesis of the glabridin in a microbial cell factory is realized for the first time, and the application prospect of the glabridin biological manufacturing industry is greatly promoted.

The invention relates to a glabridin self-assembly inclusion with an ultra-small particle size as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The composition disclosed by the invention is prepared from the following components in parts by weight: 0.5 to 5 parts of glabridin, 10 to 30 parts of polyhydric alcohol, 0.4 to 6.5 parts of liquorice root carrier component, 1.5 to 3 parts of nonionic emulsifier A, 0.45 to 1.8 parts of nonionic emulsifier B and 53.7 to 87.15 parts of water, the liquorice carrier component comprises at least one of glycyrrhizic acid, ammonium glycyrrhizinate and glycyrrhetinic acid; the nonionic emulsifier A is a nonionic emulsifier of which the hydrophilic-lipophilic balance value HLB is greater than 9; and the nonionic emulsifier B is a nonionic emulsifier with a hydrophilic-lipophilic balance value HLB of 2-4. The glabridin self-assembly inclusion disclosed by the invention is smaller in particle size, good in wrapping performance and good in targeting property; the stability is good, glabridin wrapping does not separate out, and the transdermal absorption rate is higher.

The invention discloses a synthetic method of glabridin with optical purity, which comprises the following steps: by taking (R)-3-acetoxyl-2-(2, 4-dimethoxyphenyl) propanol and 2-isopentenyl resorcinol as raw materials, carrying out Mitsunobu reaction to obtain a key intermediate compound I, then carrying out hydrolysis and iodination to obtain an intermediate compound II, and then carrying out cyclization to obtain the glabridin with optical purity. Reducing and removing a protecting group to obtain glabridin. By introducing the isopentene side chain, the reaction activity of an olefin functional group is reserved, a key site is provided for subsequent cyclization conversion, the precise spatial configuration of the product is ensured, and finally glabridin with optical purity is prepared. The method is short in route, low in cost, mild in reaction condition and suitable for industrial production.

The invention provides a glabridin flexible liposome, a glabridin flexible liposome mask liquid and a preparation method, and relates to the technical field of cosmetics. The glabridin flexible liposome provided by the invention comprises a flexible nano liposome and glabridin wrapped by the flexible nano liposome, the flexible nano liposome is a liposome modified by dipotassium glycyrrhizinate, and the liposome is formed by phospholipid and cholesterol. According to the invention, the dipotassium glycyrrhizinate is used as an edge active agent, and the glabridin is wrapped in the flexible liposome, so that the problems of poor solubility and poor stability of the glabridin are solved while the transdermal properties of the glabridin and the dipotassium glycyrrhizinate are improved. According to the glabridin flexible lipidosome mask liquid provided by the invention, the glabridin flexible lipidosome is uniformly dispersed in the mask liquid, and the obtained glabridin flexible lipidosome mask liquid has excellent stability and also has good moisturizing, soothing and whitening effects.

The invention discloses a synthetic method of glabridin, which is characterized in that 1, 3-dimethoxybenzene is used as an initial raw material, and the target product glabridin is prepared through a series of reactions. The independently researched and developed glabridin synthesis process has the advantages of cheap and easily available raw materials, simple operation, few side reactions, convenient quality control and the like, and a green organic solvent is adopted in the preparation process, so that post-treatment is simple, recycling can be realized, the emission of three wastes is less, the obtained product is high in yield and purity, and the method is suitable for industrial production and has wide application prospects. And the product has better market competitiveness.

The invention belongs to the technical field of cosmetics, and particularly relates to a glabridin composite glabridin amp. The invention discloses collagen nanoparticles as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, combining glabridin and glycyrrhizic acid through a non-covalent bond to obtain a glabridin-glycyrrhizic acid eutectic structure capable of overcoming the problem of solubility of glabridin, and then coating the glabridin-glycyrrhizic acid eutectic structure with a glabridin-collagen mixed solution to obtain the glabridin composite glabridin amp. The invention relates to collagen nanoparticles. The glabridin composite glabridin amp obtained by the method disclosed by the invention; the collagen nano-particles and the glabridin are good in water solubility, simple in component, high in stability and free of excessive allergy risks, the skin permeability of the glabridin, the collagen and the glabridin can be improved, the collagen, the glabridin and the glabridin are in synergistic interaction, and the utilization degree is improved.

The invention provides a permeation promoting composition containing PDRN and glabridin and application of the permeation promoting composition in functional skin care products. The composition comprises the following components in percentage by mass: 0.01%-5% of glabridin, 0.1%-10% of glycyrrhizic acid ester stearate, 0.01%-2% of DNA sodium, 0.1%-10% of poloxamer, 0.05%-8% of carbomer, 0.5%-5% of sodium hyaluronate and the balance of one or more of water or solvents such as butanediol, glycerol, 1.3-propylene glycol and the like. In the composition, poloxamer is self-assembled in a system to form micelles, glabridin is wrapped in an inner core, and a PEO chain segment on an outer layer is combined with hyaluronic acid through a hydrogen bond to form a molecular-level wrapping structure, so that the problem of instability of glabridin is effectively solved, meanwhile, the problem of agglomeration of glabridin can be effectively avoided, and the whitening effect of glabridin is effectively promoted. Meanwhile, the glabridin, the glycyrrhizic acid ester stearate, the DNA sodium, the poloxamer, the carbomer and the hyaluronic acid are synergistically matched, so that the composition has good whitening, anti-wrinkle and penetration promoting effects.

The invention belongs to the technical field of cosmetics, and particularly relates to whitening and freckle-removing essence oil and a preparation method thereof. According to the invention, adamantane formyl is introduced into an alpha-arbutin benzene ring to obtain the adamantane formyl modified alpha-arbutin derivative, so that the compatibility of alpha-arbutin and base oil is enhanced; the alpha-arbutin is prevented from being converted into hydroquinone by utilizing the large steric effect of adamantane formyl, so that the safety of the whitening and freckle-removing essence oil is improved; the tranexamic acid is wrapped by the ceramide nano-emulsion, so that the skin allergy risk caused by ingestion of the tranexamic acid is reduced; the ceramide nano-emulsion and tranexamic acid act together to repair skin collagenous fibers, block melanin transfer and inhibit melanin synthesis; trilinolein and glabridin are catalyzed by lipase to form a compound, so that the molecular compatibility and photo-thermal stability of glabridin are enhanced; glycerin promotes transdermal absorption of glabridin and enhances the efficacy of the whitening and freckle-removing essence oil.

The invention relates to a lipidosome based on nonapeptide-1 targeted transdermal delivery as well as a preparation method and application of the lipidosome. The lipidosome comprises a lipidosome carrier, raspberry ketone glucoside, a glabridin clathrate compound, a camellia extract and nonapeptide-1, wherein the raspberry ketone glucoside, the glabridin clathrate compound and the camellia extract are wrapped in the lipidosome carrier, and the nonapeptide-1 is modified on the surface of the lipidosome. Water-soluble raspberry ketone glucoside, a camellia extract and fat-soluble glabridin are compounded and wrapped by using a liposome technology, nonapeptide-1 which can be in targeted combination with skin melanocytes is further added, and the nonapeptide-1 is anchored on the outer side of a liposome carrier through hydrogen bond interaction, so that the liposome based on nonapeptide-1 targeted transdermal delivery is obtained. The composition solves the problem of compatibility of a fat-soluble active matter glabridin, a water-soluble active matter raspberry ketone glucoside and a camellia extract, and enhances permeation of the active matter in skin, so that the active matter directly plays a role at an action target.

This invention belongs to the field of cosmetic technology, specifically a glycyrrhizin-based composite nanoparticle containing sclerotherapy, BPOXY, and collagen, along with its preparation method and applications. The invention first combines glycyrrhizin and glycyrrhizic acid via non-covalent bonds to obtain a glycyrrhizin-glycyrrhizic acid eutectic structure that overcomes the solubility problem of glycyrrhizin. Then, a BPOXY-collagen mixture is used to coat the glycyrrhizin-glycyrrhizic acid eutectic structure, yielding glycyrrhizin-based composite nanoparticles containing sclerotherapy, BPOXY, and collagen. The glycyrrhizin-based composite nanoparticles containing sclerotherapy, BPOXY, and collagen obtained using this method exhibit good water solubility of glycyrrhizin, a simple composition, high stability, and minimal risk of allergic reactions. Furthermore, it enhances the skin permeability of glycyrrhizin, collagen, and BPOXY, resulting in a synergistic effect and improved bioavailability.

The invention discloses an anti-oxidation, whitening and brightening composition as well as a preparation method and application thereof. The anti-oxidation, whitening and brightening composition is prepared from the following components in parts by weight: 2 to 6 parts of nicotinamide, 0.5 to 5 parts of tranexamic acid, 1 to 5 parts of nonapeptide-1 solution, 0.5 to 1 part of tetrahydromethylpyrimidine carboxylic acid, 0.5 to 2 parts of pink red plum compound essence, 0.01 to 0.5 part of hydroxyethyl piperazine ethanesulfonic acid, 0.01 to 0.5 part of glabridin and 0.0001 to 0.2 part of glutathione. According to the invention, the composition prepared from nicotinamide, tranexamic acid, nonapeptide-1, tetrahydromethylpyrimidine carboxylic acid, pink-red plum composite essence, hydroxyethyl piperazine ethanesulfonic acid, glabridin and glutathione is prepared into the essence, and the clearance rate of the essence to ABTS free radicals reaches 58% or above, which shows that the essence has very good antioxidant ability; the cell tyrosinase activity inhibition rate of the composition under the concentration of 1.2% (v / v) reaches 51% or above, which shows that the composition has very good whitening and brightening effects.

The invention discloses an anti-aging and anti-allergy spray suitable for pregnant women based on micro-ecology-barrier-immune synergistic repair and a preparation method of the anti-aging and anti-allergy spray. The spray is prepared from 2.00 percent to 4.00 percent of hydrolyzed quinoa protein, 1.00 percent to 5.00 percent of lactobacillus / soybean milk fermentation filtrate, 0.30 percent to 1.00 percent of radix stellariae extract, 0.20 percent to 1.50 percent of perilla leaf extract, 0.01 percent to 0.50 percent of three-layer liposome tetrahydrocurcumin, 1.00 percent to 10.00 percent of Tibetan plateau freeze-dried tricholoma matsutake extracting solution, an active peptide combination and a ceramide compound. According to a quadruple synergistic mechanism, (1) the pH value of the fermentation filtrate is adjusted to 5.3, and the fermentation filtrate and the hydrolyzed quinoa extract synergistically construct a micro-ecological foundation; (2) repairing the barrier by ceramide NP, cholesterol, phytosphingosine and the like; (3) multiple anti-allergy effects of radices stellariae dichotomae, purple perilla, asiaticoside and dipotassium glycyrrhizinate; and (4) liposome curcumin, glabridin liposome and active peptide cooperate to resist aging, and a'micro-ecological balance-barrier reconstruction-immunosuppression-targeted anti-aging 'quadruple synergistic effect system is constructed. The preparation method comprises the steps of A-phase / B-phase preparation, emulsification, active addition and sterile filling, and the skin requirements in the pregnancy period and the lactation period are met.

This invention discloses a mild and stable temperature-responsive microemulsion composition, its preparation method, and its applications, belonging to the fields of cosmetics and pharmaceutical technology. This invention uses a nonionic decyl glucoside and anionic sodium lauroyl glutamate, introducing tocopherol polyethylene glycol succinate and disodium EDTA, forming a dual stabilizing mechanism of "antioxidant + metal ion chelation," effectively protecting unstable active ingredients such as glycyrrhizin. Experiments show that in an accelerated test at 60℃, the retention rate of glycyrrhizin increased by more than 20%. The microemulsion composition prepared by this invention exhibits high stability, low irritation, and skin-targeted sustained-release properties.

The invention provides a whitening and spot-fading skin care composition and a preparation method thereof, and belongs to the technical field of whitening and skincare, and the composition comprises modified glabridin, a nicotinamide-tranexamic acid co-coating, an alhagi sparsifolia flower extract and an artemisia desertorum seed extract. The modified glabridin is obtained by modifying glabridin with tocopherol succinate, polyethylene glycol and artemisia desertorum seed gum. The nicotinamide-tranexamic acid co-coating is obtained by coating a mixture of nicotinamide and tranexamic acid with sodium alginate and chitosan hydrochloride. The alhagi sparsifolia extract is obtained by carrying out common enzymolysis on alhagi sparsifolia dry powder through compound enzyme and glucosidase and purifying through a mixed filler adsorption column, and the artemisia desertorum seed extract is obtained by fermenting artemisia desertorum seed powder through aspergillus oryzae, removing impurities through ADS-7 resin and purifying through a polyamide resin column. The effective components are prepared by adopting a special preparation and purification method and are matched according to a specific ratio for use, and the whitening effects of inhibiting tyrosinase, blocking melanin generation, scavenging free radicals and the like are achieved through multi-effect synergism.

The invention provides a glabridin micro-emulsion, a glabridin micro-emulsion mask liquid and a preparation method, and relates to the technical field of cosmetics. The glabridin microemulsion provided by the invention is prepared from the following raw materials: glabridin, tween-80, 1, 2-hexanediol, ethyl oleate and water, and the mass ratio of the glabridin to the tween-80 to the 1, 2-hexanediol to the ethyl oleate to the water is (0.25 to 2.5) to (12.5 to 25) to (12.5 to 50) to (5 to 12.5) to 19. According to the glabridin microemulsion, 1, 2-hexanediol is used as a surfactant, tween-80 is used as a cosurfactant, ethyl oleate is used as an oil phase, glabridin is wrapped in the microemulsion, the solubility and skin permeability of glabridin can be improved, and cosmetics can efficiently play skin care functions such as whitening and relieving. The invention provides a glabridin micro-emulsion mask liquid which has good whitening, moisturizing and soothing effects.

The application discloses a glabridin-nicotinamide co-crystal as well as a preparation method and application thereof, belongs to the technical field of co-crystals, and comprises nicotinamide and glabridin molecules. 26 H 26 N2O5, and the molar ratio of the nicotinamide and the glabridin is 1:1.The application prepares a glabridin-nicotinamide co-crystal, and the co-crystal is completely characterized, and it is proved that the co-crystal has certain physical and chemical stability, the stability of the nicotinamide is improved after being made into the co-crystal, and no stimulating substance nicotinic acid is found in the co-crystal stability experiment. The co-crystal is generally neutral, mild and non-irritating, and is naturally suitable for cosmetics or health products.

The present invention relates to a self-assembled inclusion of glabridin with an ultra-small particle size, a preparation method, and an application thereof, and belongs to the field of cosmetic technology. The composition of the present invention comprises the following components in parts by weight: 0.5-5 parts of glabridin, 10-30 parts of polyol, 0.4-6.5 parts of a licorice carrier component, 1.5-3 parts of a nonionic emulsifier A, 0.45-1.8 parts of a nonionic emulsifier B, and 53.7-87.15 parts of water; the licorice carrier component comprises at least one of glycyrrhizic acid, ammonium glycyrrhizate, and glycyrrhetinic acid; the nonionic emulsifier A is a nonionic emulsifier with a hydrophilic-lipophilic balance value (HLB) greater than 9; the nonionic emulsifier B is a nonionic emulsifier with a hydrophilic-lipophilic balance value (HLB) of 2-4. The self-assembled inclusion of glabridin of the present invention has a smaller particle size, good encapsulation performance, and good targeting; it has good stability, the glabridin encapsulation does not precipitate, and the transdermal absorption rate is higher.

The application belongs to the technical field of glabridin preparation, and particularly relates to a preparation method of R-glabridin, which comprises the following steps: S1, under a protective gas atmosphere, dissolving a glabridin racemate in an organic solvent A, and reacting with a halogenated reagent through a substitution reaction, and after purification, preparing a halogenated intermediate I; S2, under the protective gas atmosphere, dissolving the halogenated intermediate I prepared in S1 in an organic solvent B, and under alkaline conditions, preparing an intermediate II through a dehalogen olefination reaction, and after purification; S3, under a hydrogen atmosphere, dissolving the intermediate II prepared in S2 in an organic solvent C, and under the action of a catalyst, preparing R-glabridin through an asymmetric hydrogenation reaction, and after purification. The method has the advantages of multiple raw material sources, obvious cost advantage, convenient preparation method, high total yield, less waste and high purity, and is beneficial to industrialization.

The present application relates to a kind of moisturizing whitening anti-aging cosmetic composition containing hyaluronic acid derivative and glabridin and its application, belong to the field of cosmetics.The cosmetic composition includes glabridin and hyaluronic acid derivative;Wherein, the hyaluronic acid derivative includes hydrolyzed hyaluronic acid or its salt, acetylated hyaluronic acid or its salt and silanized hyaluronic acid or its salt.The present application simultaneously uses hydrolyzed hyaluronic acid or its salt, acetylated hyaluronic acid or its salt and silanized hyaluronic acid or its salt as hyaluronic acid derivative, greatly improve the stability and moisturizing, whitening, anti-aging efficacy of the cosmetic composition provided by the present application, with unexpected synergistic effect.

This invention belongs to the field of pesticide and fertilizer technology, specifically a pesticide and fertilizer for controlling papaya gall disease. It includes a synergistic bactericidal composition, the active ingredient of which is a combination of glycyrrhizin and fluoxastrobin. This pesticide and fertilizer for controlling papaya gall disease contains both bactericidal and boron fertilizer. The bactericidal component enhances the control effect against papaya anthracnose, while the boron fertilizer supplements the boron element needed for papaya growth, thus effectively preventing papaya gall disease. This single application can control both anthracnose and papaya gall disease, promoting the normal and healthy growth of papaya.

The invention provides 2-oxoglutaric acid dependent dioxygenase 2-ODD of glycyrrhiza glabra as well as a coding gene and application of the 2-oxoglutaric acid dependent dioxygenase 2-ODD. The coding gene of the 2-oxoglutaric acid dependent dioxygenase 2-ODD of the glycyrrhiza glabra, which is provided by the invention, has a nucleotide sequence as shown in SEQ ID NO. 1. The protein coded by the Gg2-ODD can be used for catalytically synthesizing glabridin or a precursor compound thereof. The synthesis method disclosed by the invention is economical, efficient, green and environment-friendly, and the synthesized glabridin or the precursor thereof is high in purity. The enzyme participates in synthesis of glabridin, provides an important element for synthetic organisms of glabridin, and also provides a basis for discussing a regulation mechanism of glabridin synthesis in the future.

The present application relates to the field of skin care products, and discloses a whitening and anti-aging composition for skin care products and application thereof, and an essence oil and a preparation method thereof. The whitening and anti-aging composition for skin care products contains an active substance combination and a compound essential oil and plant oil combination; the active substance combination contains glabridin, 4-butyl resorcinol, undecylenoyl phenylalanine, natural bisabalol, ascorbic acid tetraisopalmitate and teprenone; the compound essential oil and plant oil combination contains Damascus rose essential oil, magnolia essential oil, frankincense essential oil, Roman chamomile essential oil, carrot seed essential oil, passion fruit seed oil, red wolfberry seed oil, rose fruit oil and sea buckthorn fruit oil. The skin care product prepared from the whitening and anti-aging composition has a more moderate and efficient transdermal absorption rate, and has excellent anti-photoaging effect and whitening and freckle-removing effect.

The present disclosure relates to the technical field of pharmaceutical preparation, and particularly relates to a preparation method of glabriden; the preparation method comprises the following steps: providing licorice raw materials, crushing and sieving, and then performing a solid state fermentation process to obtain licorice powder after solid state fermentation; then adding into a first mixed organic solution for soaking, and then adding into a first mixed aqueous solution for soaking, and then adding a second mixed organic solution for extraction, collecting the organic phase, and then adding the solid product into the second mixed organic solution for extraction after enzyme inactivation and impurity removal; combining the organic phases and performing dialysis, purification and concentration to obtain a licorice flavone extract; and then performing extraction of the licorice flavone extract by a third mixed organic solution containing imidazolyl sulfonate to obtain a product containing a higher proportion of glabriden.

The present invention discloses a method for synthesizing optically pure glabridin. The method uses (R)-3-acetoxy-2-(2,4-dimethoxyphenyl)propanol and 2-isopentenylresorcinol as raw materials to obtain a key intermediate compound I through a Mitsunobu reaction, and then obtains an intermediate compound II through hydrolysis and iodination. The intermediate compound II is then cyclized, reduced, and protected by removal to obtain glabridin. The present invention retains the reactivity of the olefin functional group by introducing an isopentyl side chain, provides a key site for subsequent cyclization, ensures the precise stereoconfiguration of the product, and ultimately obtains optically pure glabridin. The present invention has a short route, low cost, mild reaction conditions, and is suitable for industrial production.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a method for simultaneously extracting and purifying glabridin, glycyrrhizic acid and isoliquiritigenin. The method comprises the following steps: (1) adding ethanol into glycyrrhiza glabra, and extracting to obtain an extracting solution; (2) extracting the extracting solution with ethyl acetate to obtain an organic phase and a water phase; and (3) purifying the organic phase by using a rapid preparative liquid chromatograph, carrying out gradient elution by using 30%-70% of ethyl acetate-cyclohexane as a mobile phase, and collecting the eluent, thereby obtaining the glabridin. (4) carrying out acidification and centrifugal precipitation on the water phase to obtain a glycyrrhizic acid crude product and a supernatant, adding absolute ethyl alcohol into the glycyrrhizic acid crude product, and carrying out ultrasonic extraction and centrifugation to obtain a glycyrrhizic acid ethanol solution; and (5) extracting and purifying the supernate to obtain the isoliquiritigenin.

The application discloses a recombinant engineering strain for biosynthesis of glabridin and a construction method and application thereof, and belongs to the technical field of synthetic biology and enzyme engineering. The recombinant engineering strain comprises a recombinant plasmid pRSFDuet-SiDR-SiTR-PmGDH; wherein the nucleotide sequence of SiTR is shown as SEQ ID NO:1, the nucleotide sequence of SiDR is shown as SEQ ID NO:2, and the nucleotide sequence of PmGDH is shown as SEQ ID NO:3. Through technical means such as optimization of gene sequences, construction of expression vectors and in-vivo directed evolution, the application successfully constructs the recombinant functional strain pTDG- E. coli BL21(DE3) and its evolution strain pTDGm258- E. coli BL21(DE3). The strains can efficiently catalyze the conversion of a substrate into glabridin, and have important industrial application value.

The present invention relates to the field of cosmetics, and in particular to a supramolecular eutectic gel that efficiently targets MC1R, and its preparation and application. The supramolecular eutectic gel that efficiently targets MC1R according to the present invention comprises the following components in parts by weight: 1-5 parts of glabridin, 1-6.5 parts of glycyrrhetinic acid, 3-23 parts of C3-C4 alcohol, and 0.001-0.1 parts of a targeting active substance. The present invention compounds glabridin and glycyrrhetinic acid, with glycyrrhetinic acid serving as a hydrogen bond acceptor and glabridin serving as a hydrogen bond donor. In a small amount of C3-C4 alcohol, supramolecular eutectics can be formed through π-π stacking and hydrogen bonding, thereby improving the stability and skin permeability of glabridin. The targeting active substance has a significant binding effect with MC1R, achieving the effect of synergistically promoting efficient whitening. The present invention combines supramolecular eutectic and nanoliposome technology to obtain whitening liposomes, which have excellent whitening efficacy, clear targets, efficient penetration, high utilization rate, and are easy to mass produce.

The invention discloses a biological whitening composition containing milk exosome as well as a preparation method and application of the biological whitening composition. According to the milk exosome composition, multiple compounded emulsion emulsifiers are adopted, the vesicle structure of the milk exosome can be protected, the milk exosome can stably exist in the composition, and therefore the whitening effect is stably achieved. In addition, by adding the glabridin and the daisy flower extract and cooperating with the milk exosome, a stronger whitening effect is achieved, the glabridin has the effects of inhibiting melanin and resisting inflammation and accurately plays a whitening role, and the daisy flower extract can achieve the whitening effect from the source by reducing melanin generation. The whitening effects of the whitening compositions jointly play a role in inhibiting melanogenesis genes, reducing melanogenesis and preventing melanin transfer and transfer, and the whitening effects are played from various ways.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a method for simultaneously extracting and purifying glabridin, glycyrrhizic acid and isoliquiritigenin by a column chromatography extraction method. The method comprises the following steps: adding an ethanol solution into glycyrrhiza glabra, extracting by adopting a column chromatography extraction method, extracting an extracting solution by using dichloroethane, enabling an organic phase to pass through a silica gel column, and crystallizing an eluent to obtain glabridin. Acidifying and centrifuging the extracted water phase, and dissolving the precipitate with acetone to obtain glycyrrhizic acid; and extracting and purifying the filtrate to obtain the isoliquiritigenin. The invention provides a simple and efficient new extraction technology which is low in energy consumption, small in solvent dosage and high in extraction rate compared with traditional ultrasonic extraction and the like, and aims to extract glycyrrhizic acid and glabridin in glycyrrhiza glabra at the same time, separate glycyrrhizic acid and glabridin, separate isoliquiritigenin from the remaining part through simple conversion, and improve the purity of glycyrrhizic acid and glabridin in glycyrrhiza glabra. The comprehensive development and utilization of the glycyrrhiza glabra are achieved.