Skin care composition with whitening effect and application thereof

Abstract

The invention relates to a skin care composition with a whitening effect and application of the skin care composition. The skin care composition is prepared from a plant extract, nicotinamide adenine dinucleotide, tetrahydrocurcumin, trehalose, nicotinamide and phenylethyl resorcinol, the plant extract comprises any one or a combination of at least two of a white truffle extract, a perigord truffle extract, a chlorella extract, glabridin or alpha-arbutin. The plant extract, the nicotinamide adenine dinucleotide, the tetrahydrocurcumin, the trehalose, the nicotinamide and the phenethyl resorcinol are creatively combined and matched for use, a synergistic interaction effect is achieved in the whitening aspect, and the composition is mild in performance, free of irritation, safe and free of toxic and side effects and has a good whitening effect after being applied to cosmetic products. The skin whitening effect can be obviously improved, and the good use feeling is achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a skin care composition with whitening effect and application thereof. Background Art

[0002] With the development and progress of society, people are paying more and more attention to the external manifestations of their personal image and health status. As the saying goes, a white complexion can hide a hundred uglinesses, so fair, tender and smooth skin can better enhance a person's external image. As a result, whitening skin care products have also been favored by consumers.

[0003] At present, there are many whitening skin care products. CN118453478A discloses a whitening composition, an extraction method, an application and a skin care product. The whitening composition comprises extracts of radix rutaecarpa, mulberry root, astragalus and emblica fruit, wherein the weight ratio of radix rutaecarpa, mulberry root, astragalus and emblica fruit is 1-10:1-10:1-10:1-10. The present invention compounds the extracts of radix rutaecarpa, mulberry root, astragalus and emblica fruit, and the functional components of multiple whitening mechanisms synergistically improve the whitening effect of the composition.

[0004] CN118697670A discloses a whitening composition, a whitening skin care product and a preparation method thereof. The whitening composition comprises: a thallus extract of P. truncatula and a protease. The thallus extract of P. truncatula inhibits the production of melanin in the body and promotes the metabolism of the stratum corneum; the protease can gently exfoliate the keratin and promote the renewal of the stratum corneum; wherein the keratinocytes fall off with the melanin, thereby solving the problem of pigment deposition by inhibiting the production of melanin and shedding melanin, and enhancing the skin whitening effect of whitening cosmetics. Moreover, the protease in the composition is similar in structure to the human body's own trypsin and will not damage the skin itself. The thallus extract of P. truncatula is a natural product extract, so the composition has low irritation and is suitable for people with sensitive skin.

[0005] At present, although there are more and more whitening products on the market, they all have certain shortcomings. The active ingredients are difficult to be effectively absorbed by the skin, and the whitening effect often fails to achieve the expected effect. Therefore, how to provide a whitening product that can further achieve better whitening effects and is safe and non-irritating has become a problem that needs to be solved urgently. Summary of the invention

[0006] In view of the deficiencies of the prior art, the object of the present invention is to provide a skin care composition with whitening effect and its application.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a skin care composition with whitening effect, the skin care composition comprising a plant extract, nicotinamide adenine dinucleotide, tetrahydrocurcumin, trehalose, nicotinamide and phenethylresorcinol;

[0009] The plant extract comprises any one of white truffle extract, black truffle extract, galactotrophic algae extract, glabridin or α-arbutin, or a combination of at least two thereof.

[0010] The present invention creatively combines plant extracts, nicotinamide adenine dinucleotide, tetrahydrocurcumin, trehalose, nicotinamide and phenylethyl resorcinol. The six are used in combination to have a synergistic effect in whitening, can effectively reduce the transport of melanin to cells, accelerate the metabolism of melanin, inhibit the activity of cells synthesizing melanin, improve uneven skin color, and will not turn black again after use. In addition, the composition provided by the present invention is mild and non-irritating, safe and has no toxic side effects. When used in cosmetic products, it can not only significantly improve the skin whitening effect, but also has a good sense of use.

[0011] Preferably, the plant extract comprises a combination of white truffle extract, black truffle extract, glabridin, galactoside extract and α-arbutin.

[0012] The present invention provides a plant composition using white truffle extract, black truffle extract, glycyrrhizin, galactoglobulus extract and α-arbutin, which have a synergistic effect, can regulate skin pigmentation, inhibit pigment synthesis and transport, and thus can better improve the whitening effect of the composition.

[0013] Preferably, the mass ratio of the white truffle extract, the black truffle extract, glabridin, the galactotrophic algae extract and α-arbutin is (1-2):(0.5-1.5):(1-3):(0.1-1):(1-2).

[0014] The first "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0015] “0.5-1.5” can mean 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3 or 1.4, etc.;

[0016] "1-3" can be 1.1, 1.3, 1.5, 1.7, 1.9, 2, 2.1, 2.3, 2.5, 2.7 or 2.9, etc.;

[0017] “0.1-1” can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 or 0.9, etc.;

[0018] The second "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.

[0019] Preferably, the Chlorella extract comprises Chlorella exosomes and / or Haematococcus pluvialis extract.

[0020] Preferably, the skin care composition further comprises a vitamin derivative.

[0021] Preferably, the vitamin derivative comprises any one of 3-o-ethyl ascorbic acid, ascorbyl tetraisopalmitate or tranexamic acid, or a combination of at least two thereof.

[0022] Preferably, the vitamin derivative is a combination of 3-o-ethyl ascorbic acid, ascorbyl tetraisopalmitate and tranexamic acid.

[0023] As a preferred technical solution of the present invention, the combination of 3-o-ethyl ascorbic acid, ascorbyl tetraisopalmitate and tranexamic acid can better inhibit the synthesis of melanin, accelerate the metabolism of melanin, and thus make the whitening effect of the composition more significant.

[0024] Preferably, the mass ratio of 3-o-ethyl ascorbic acid, ascorbyl tetraisopalmitate and tranexamic acid is (0.1-2):(1-3):(0.5-1.5).

[0025] Wherein “0.1-2” can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0026] "1-3" can be 1.1, 1.3, 1.5, 1.7, 1.9, 2, 2.1, 2.3, 2.5, 2.7 or 2.9, etc.;

[0027] “0.5-1.5” can mean 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3 or 1.4, etc.

[0028] Preferably, the skin care composition with whitening effect also includes a polypeptide.

[0029] Preferably, the polypeptide comprises carnosine and / or glutathione.

[0030] Preferably, the polypeptide comprises carnosine and glutathione.

[0031] Preferably, the mass ratio of carnosine to glutathione is 1:(2-3), for example, it can be 1:2.1, 1:2.2, 1:2.3, 1:2.4, 1:2.5, 1:2.6, 1:2.7, 1:2.8 or 1:2.9, etc.

[0032] Preferably, the skin care composition with whitening effect comprises plant extracts, nicotinamide adenine dinucleotide, tetrahydrocurcumin, trehalose, nicotinamide, phenethylresorcinol, vitamin derivatives and polypeptides.

[0033] Preferably, the skin care composition with whitening effect comprises, by weight, 4-10 parts of plant extracts, 1-3 parts of nicotinamide adenine dinucleotide, 2-4 parts of tetrahydrocurcumin, 4-8 parts of trehalose, 2-5 parts of nicotinamide, 0.1-2 parts of phenethylresorcinol, 1-2 parts of vitamin derivatives and 1-3 parts of polypeptides.

[0034] The amount of the plant extract added to the skin care composition with whitening effect of the present invention can be 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts or 9.5 parts, etc.;

[0035] The amount of nicotinamide adenine dinucleotide added can be 1.1 parts, 1.3 parts, 1.5 parts, 1.7 parts, 1.9 parts, 2 parts, 2.1 parts, 2.3 parts, 2.5 parts, 2.7 parts or 2.9 parts, etc.;

[0036] The added amount of the tetrahydrocurcumin can be 2.1 parts, 2.3 parts, 2.5 parts, 2.7 parts, 2.9 parts, 3 parts, 3.1 parts, 3.3 parts, 3.5 parts, 3.7 parts or 3.9 parts, etc.;

[0037] The added amount of trehalose can be 4.2 parts, 4.5 parts, 4.8 parts, 5 parts, 5.2 parts, 5.5 parts, 5.8 parts, 6 parts, 6.2 parts, 6.5 parts, 6.8 parts, 7 parts, 7.2 parts, 7.5 or 7.8 parts, etc.;

[0038] The addition amount of 2-5 parts of niacinamide can be 2.1 parts, 2.3 parts, 2.5 parts, 2.8 parts, 3 parts, 3.2 parts, 3.5 parts, 3.7 parts, 3.9 parts, 4 parts, 4.2 parts, 4.5 parts, 4.7 parts or 4.9 parts, etc.;

[0039] The amount of phenethyl resorcinol added can be 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 parts, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts or 1.9 parts, etc.;

[0040] The added amount of the vitamin derivative can be 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts or 1.9 parts, etc.;

[0041] The added amount of the polypeptide can be 1.1 parts, 1.3 parts, 1.5 parts, 1.7 parts, 1.9 parts, 2 parts, 2.1 parts, 2.3 parts, 2.5 parts, 2.7 parts or 2.9 parts, etc.

[0042] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0043] In a second aspect, the present invention provides a use of the skin care composition with whitening effect as described in the first aspect in the preparation of cosmetics.

[0044] Preferably, the mass percentage of the composition with whitening effect in the cosmetic is 1-20%, for example, it can be 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18% or 19%, etc.

[0045] Preferably, the cosmetics include any one of essence, essence cream, lotion, facial mask or gel.

[0046] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0047] In a third aspect, the present invention provides a lotion, wherein the raw materials of the lotion include the skin care composition with whitening effect as described in the first aspect and water;

[0048] Preferably, the raw material composition of the lotion further includes additives, and the additives include any one of moisturizers, emulsifiers, preservatives, fragrances or thickeners, or a combination of at least two of them.

[0049] Preferably, the moisturizing agent includes any one of butylene glycol, glycerol, 1,2-hexanediol, hyaluronic acid, sorbitol or xylitol, or a combination of at least two thereof.

[0050] Preferably, the emulsifier includes any one of polysorbate-20, polysorbate-60, polyglyceryl-2 dipolyhydroxystearate, coco-glucoside or PEG-10 dimethicone, or a combination of at least two thereof.

[0051] Preferably, the thickener comprises any one of carbomer, xanthan gum, guar gum or ammonium acryloyldimethyltaurate / VP copolymer or a combination of at least two thereof.

[0052] Preferably, the preservative includes any one of phenoxyethanol, potassium sorbate or ethylhexylglycerin, or a combination of at least two thereof.

[0053] Compared with the prior art, the present invention has the following beneficial effects:

[0054] The present invention creatively combines plant extracts, nicotinamide adenine dinucleotide, tetrahydrocurcumin, trehalose, nicotinamide and phenylethyl resorcinol. The six are used in combination to have a synergistic effect in whitening, can effectively reduce the transport of melanin to cells, accelerate the metabolism of melanin, inhibit the activity of cells synthesizing melanin, improve uneven skin color, and will not turn black again after use. In addition, the composition provided by the present invention is mild and non-irritating, safe and has no toxic side effects. When used in cosmetic products, it can not only significantly improve the skin whitening effect, but also has a good sense of use. DETAILED DESCRIPTION

[0055] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0056] The weight percentages involved in the following embodiments and comparative examples are all calculated based on the actual effective ingredients contained in the commercially available raw materials. The commercially available raw materials may also selectively contain any one or a combination of at least two of solvents, fillers, diluents, stabilizers, pH adjusters, antibacterial agents, antioxidants or impurities within the permitted range.

[0057] The following materials and comparative examples are as follows:

[0058]

[0059]

[0060] The rest of the raw materials can be used as long as they are purchased from regular dealers.

[0061] Example 1

[0062] The present embodiment provides a skin care composition with whitening effect, wherein the skin care composition with whitening effect comprises, by weight, 7 parts of plant extract, 2 parts of nicotinamide adenine dinucleotide, 3 parts of tetrahydrocurcumin, 6 parts of trehalose, 4 parts of nicotinamide, 1.5 parts of phenethylresorcinol, 1.5 parts of vitamin derivatives, 2 parts of polypeptides, and water is added to 50 parts;

[0063] The plant extracts are white truffle extract, black truffle extract, glabridin, globulus extract and α-arbutin in a mass ratio of 1.5:1:2:0.5:1.5;

[0064] The chlorella extract is a chlorella exosome and a Haematococcus pluvialis extract in a mass ratio of 1:1;

[0065] The vitamin derivatives are 3-o-ethyl ascorbic acid, ascorbyl tetraisopalmitate and tranexamic acid in a mass ratio of 1:2:1;

[0066] The polypeptides are carnosine and glutathione in a mass ratio of 1:2.5.

[0067] The preparation method of the skin care composition with whitening effect is:

[0068] The skin care composition with whitening effect is obtained by mixing plant extracts, nicotinamide adenine dinucleotide, tetrahydrocurcumin, trehalose, nicotinamide, phenethylresorcinol, vitamin derivatives and polypeptides in water.

[0069] Example 2

[0070] The present embodiment provides a skin care composition with whitening effect, wherein the skin care composition with whitening effect comprises, by weight, 10 parts of plant extract, 1 part of nicotinamide adenine dinucleotide, 4 parts of tetrahydrocurcumin, 4 parts of trehalose, 4.5 parts of nicotinamide, 0.5 parts of phenethylresorcinol, 2 parts of vitamin derivatives, and 1 part of polypeptide, and water is added to 50 parts;

[0071] The plant extracts are white truffle extract, black truffle extract, glabridin, Chlorella exosomes and α-arbutin in a mass ratio of 2:0.5:3:0.3:2;

[0072] The vitamin derivatives are 3-o-ethyl ascorbic acid, ascorbyl tetraisopalmitate and tranexamic acid in a mass ratio of 2:1:1.5;

[0073] The polypeptides are carnosine and glutathione in a mass ratio of 1:3.

[0074] The preparation method of the skin care composition with whitening effect is as shown in Example 1.

[0075] Example 3

[0076] The present embodiment provides a skin care composition with whitening effect, wherein the skin care composition with whitening effect comprises, by weight, 6 parts of plant extract, 3 parts of nicotinamide adenine dinucleotide, 2 parts of tetrahydrocurcumin, 8 parts of trehalose, 2 parts of nicotinamide, 2 parts of phenethyl resorcinol, 1 part of vitamin derivatives, 3 parts of polypeptides, and water is added to 50 parts;

[0077] The plant extracts are white truffle extract, black truffle extract, glabridin, Haematococcus pluvialis extract and α-arbutin in a mass ratio of 1:1.5:1:1:1;

[0078] The vitamin derivatives are 3-o-ethyl ascorbic acid, ascorbyl tetraisopalmitate and tranexamic acid in a mass ratio of 0.5:3:0.5;

[0079] The polypeptides are carnosine and glutathione in a mass ratio of 1:2.

[0080] The preparation method of the skin care composition with whitening effect is as shown in Example 1.

[0081] Example 4

[0082] This embodiment provides a skin care composition with whitening effect. The only difference between the skin care composition with whitening effect and Example 1 is that the components do not include vitamin derivatives, and the reduced amount is supplemented by the components in the polypeptide according to the mass ratio. The remaining components and addition amounts are consistent with Example 1, and the preparation method refers to Example 1.

[0083] Example 5

[0084] This embodiment provides a skin care composition with whitening effect. The difference between the skin care composition with whitening effect and Example 1 is that the vitamin derivative is 3-o-ethyl ascorbic acid and ascorbyl tetraisopalmitate in a mass ratio of 1:2, and the added amount of the vitamin derivative is kept unchanged. The other components and added amounts are consistent with Example 1, and the preparation method refers to Example 1.

[0085] Example 6

[0086] This embodiment provides a skin care composition with whitening effect. The difference between the skin care composition with whitening effect and Example 1 is that the vitamin derivatives are 3-o-ethyl ascorbic acid and tranexamic acid in a mass ratio of 1:1, and the added amount of the vitamin derivatives is kept unchanged. The other components and added amounts are consistent with Example 1, and the preparation method refers to Example 1.

[0087] Example 7

[0088] This embodiment provides a skin care composition with whitening effect. The difference between the skin care composition with whitening effect and Example 1 is that the vitamin derivatives are ascorbic acid tetraisopalmitate and tranexamic acid in a mass ratio of 2:1, and the added amount of the vitamin derivatives is kept unchanged. The other components and added amounts are consistent with Example 1, and the preparation method refers to Example 1.

[0089] Example 8

[0090] This embodiment provides a skin care composition with whitening effect. The only difference between the skin care composition with whitening effect and Example 1 is that the components do not include polypeptides, and the reduced amount is supplemented by the components in the vitamin derivatives according to the mass ratio. The remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0091] Example 9

[0092] This embodiment provides a skin care composition with whitening effect. The difference between the skin care composition with whitening effect and Example 1 is that the polypeptide is only carnosine, and the added amount of the polypeptide is kept unchanged. The other components and the added amounts are consistent with Example 1. The preparation method refers to Example 1.

[0093] Example 10

[0094] This embodiment provides a skin care composition with whitening effect. The difference between the skin care composition with whitening effect and Example 1 is that the polypeptide is only glutathione, and the added amount of the polypeptide is kept unchanged. The other components and the added amounts are consistent with Example 1. The preparation method refers to Example 1.

[0095] Embodiment 11

[0096] The present embodiment provides a skin care composition with whitening effect. The difference between the skin care composition with whitening effect and Example 1 is that the plant extract is black truffle extract, glycyrrhizin, chlorella extract and α-arbutin in a mass ratio of 1:2:0.5:1.5, and the added amount of the plant extract is kept unchanged. The other components and added amounts are consistent with Example 1. The preparation method refers to Example 1.

[0097] Example 12

[0098] The present embodiment provides a skin care composition with whitening effect. The difference between the skin care composition with whitening effect and Example 1 is that the plant extract is white truffle extract, glycyrrhizin, chlorella extract and α-arbutin in a mass ratio of 1.5:2:0.5:1.5, and the added amount of the plant extract is kept unchanged. The other components and added amounts are consistent with Example 1. The preparation method refers to Example 1.

[0099] Embodiment 13

[0100] The present embodiment provides a skin care composition with whitening effect. The difference between the skin care composition with whitening effect and Example 1 is that the plant extracts are white truffle extract, black truffle extract, chlorella extract and α-arbutin in a mass ratio of 1.5:1:0.5:1.5, and the added amount of the plant extract is kept unchanged. The other components and added amounts are consistent with Example 1. The preparation method refers to Example 1.

[0101] Embodiment 14

[0102] The present embodiment provides a skin care composition with whitening effect. The difference between the skin care composition with whitening effect and Example 1 is that the plant extract is white truffle extract, black truffle extract, glycyrrhizin and α-arbutin in a mass ratio of 1.5:1:2:1.5, and the added amount of the plant extract is kept unchanged. The other components and added amounts are consistent with Example 1. The preparation method refers to Example 1.

[0103] Embodiment 15

[0104] The present embodiment provides a skin care composition with whitening effect. The difference between the skin care composition with whitening effect and Example 1 is that the plant extracts are white truffle extract, black truffle extract, glycyrrhizin and sphaerocephala extract in a mass ratio of 1.5:1:2:0.5, and the added amount of the plant extracts is kept unchanged. The other components and added amounts are consistent with Example 1. The preparation method refers to Example 1.

[0105] Comparative Example 1

[0106] This comparative example provides a skin care composition with whitening effect. The skin care composition with whitening effect is different from Example 1 only in that the plant extract is not included in the components, and the reduced amount is distributed to nicotinamide adenine dinucleotide, tetrahydrocurcumin, trehalose, nicotinamide and phenethylresorcinol according to the proportion of parts. The remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0107] Comparative Example 2

[0108] This comparative example provides a skin care composition with whitening effect. The skin care composition with whitening effect is different from Example 1 only in that nicotinamide adenine dinucleotide is not included in the components, and the reduced amount thereof is distributed to the plant extract, tetrahydrocurcumin, trehalose, nicotinamide and phenethylresorcinol according to the proportion of parts. The remaining components and the addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0109] Comparative Example 3

[0110] This comparative example provides a skin care composition with whitening effect. The skin care composition with whitening effect is different from Example 1 only in that tetrahydrocurcumin is not included in the components, and the reduced amount thereof is distributed to the plant extract, nicotinamide adenine dinucleotide, trehalose, nicotinamide and phenethylresorcinol according to the proportion of parts. The remaining components and the addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0111] Comparative Example 4

[0112] This comparative example provides a skin care composition with whitening effect. The skin care composition with whitening effect is different from Example 1 only in that trehalose is not included in the components, and the reduced amount thereof is distributed to the plant extract, nicotinamide adenine dinucleotide, tetrahydrocurcumin, nicotinamide and phenethylresorcinol according to the proportion of parts. The remaining components and the addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0113] Comparative Example 5

[0114] This comparative example provides a skin care composition with whitening effect. The skin care composition with whitening effect is different from Example 1 only in that niacinamide is not included in the components, and the reduced amount thereof is distributed to the plant extract, nicotinamide adenine dinucleotide, tetrahydrocurcumin, trehalose and phenethylresorcinol according to the proportion of parts. The remaining components and the addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0115] Comparative Example 6

[0116] This comparative example provides a skin care composition with whitening effect. The skin care composition with whitening effect is different from Example 1 only in that phenethyl resorcinol is not included in the components, and the reduced amount thereof is distributed to the plant extract, nicotinamide adenine dinucleotide, tetrahydrocurcumin, trehalose and nicotinamide according to the proportion of parts. The remaining components and the addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0117] Test Example 1: Intracellular tyrosinase inhibition test

[0118] Test objects: samples of Examples 1-15 and Comparative Examples 1-6.

[0119] Test method:

[0120] 1.1 Sample preparation

[0121] The sample was prepared at a ratio of 0.45 mg sample to 1 ml serum-free culture medium;

[0122] 1.2 Cell culture

[0123] Mouse B16 melanoma cells were grown to a confluent state, digested with trypsin-EDTA for 5 minutes, and then dispersed by blowing. The cell suspension was aspirated into a centrifuge tube and centrifuged at 1000r / min for 5 minutes. The supernatant was discarded and culture medium was added to obtain a cell suspension. The cell suspension was diluted to a certain concentration with culture medium. B16 cells in the logarithmic growth phase were inoculated in a 96-well plate at a cell density of 7×10 4 cfu / ml, 100 μL per well, mark the top cover of the cell plate after laying the plate, and culture in the incubator for 24 hours.

[0124] After 24 hours of culture, the cell plate was carefully aspirated with a pipette to remove the supernatant and divided into a blank group, a positive control group, and a sample group. The prepared sample was added to the sample group, the culture solution containing α-arbutin was added to the positive control group, and the serum-free culture medium without drugs was added to the blank group. The addition amount was 100 μL / well, and 4 replicates were made for each concentration. After addition, the cells were placed in an incubator for 72 hours of culture.

[0125] 1.3 Determination of intracellular tyrosinase inhibition rate

[0126] After incubation for 72 hours, the supernatant was discarded, 150 μL of 1% TritonX-100 solution was added to each well, and the cells were quickly frozen at -80°C for 30 minutes, then thawed at room temperature to completely rupture the cells, and 60 μL of 0.5% L-DOPA solution was added after preheating at 37°C for 5 minutes, and reacted at 37°C for 2 hours. The absorbance (OD) value was measured at 490 nm on a microplate reader. The tyrosinase activity inhibition rate was calculated.

[0127] Tyrosinase activity inhibition rate (%) = (1-average absorbance value of sample / average absorbance value of blank) × 100%. The results are shown in Table 1.

[0128] Test Example 2: Intracellular melanin inhibition test

[0129] Test objects: samples of Examples 1-15 and Comparative Examples 1-6.

[0130] Test method:

[0131] 2.1 Sample preparation

[0132] The sample composition was sterilized by filtration and prepared at a ratio of 0.45 mg sample to 1 ml cell culture medium;

[0133] 2.2 Cell culture

[0134] Mouse B16 melanoma cells were grown to a confluent state, digested with trypsin-EDTA for 5 minutes, and then dispersed by blowing. The cell suspension was aspirated into a centrifuge tube and centrifuged at 1000 r / min for 5 minutes. The supernatant was discarded and the culture medium was added to obtain a cell suspension. The cell suspension was diluted to a certain concentration with the culture medium. Cells in the logarithmic growth phase were inoculated in a 6-well plate at a cell density of 1×10 4 cfu / ml, 2 mL per well, mark the top cover of the cell plate after laying the plate, and culture in the incubator for 24 h.

[0135] After the cells were cultured for 24 hours, they were divided into a sample group and a blank group. The cell culture medium of the sample group was replaced with a culture medium containing the test sample, and the blank group was added with culture medium, 2 mL per well, and cultured in a 37°C, 5% CO2 incubator for 48 hours; after 48 hours, the culture medium was replaced, 3 ml of culture medium containing the sample was added to the sample group, and the blank group was added with culture medium, and cultured in a 37°C, 5% CO2 incubator for 72 hours; after 72 hours of culture, the culture medium was replaced, 3 ml of culture medium containing the sample was added to the sample group, and the blank group was added with culture medium, and cultured in a 37°C, 5% CO2 incubator for another 48 hours.

[0136] 2.3 Determination of intracellular melanin inhibition rate

[0137] After the cell culture in 2.2 is completed, wash twice with PBS, remove the PBS, and add 200μL 0.25% trypsin to each well to digest the cells for 5 minutes. Add 2mL PBS twice to blow the cells, collect the cells into a centrifuge tube, and centrifuge at 4000rpm for 5 minutes. Try to suck out the PBS residue in the centrifuge tube, add 200μL of melanin extract to each tube, shake evenly, and heat in an 80℃ water bath for 1h. After cooling, centrifuge the droplets on the tube wall moderately, blow evenly, and absorb 150μL of the solution in each centrifuge tube, transfer to a 96-well plate, and detect the absorbance of each well at 405nm with an enzyme reader.

[0138] The cell melanin inhibition rate (%) = (1-average absorbance value of sample / average absorbance value of blank) × 100%. The results are shown in Table 1.

[0139] Test Example 3: Extracellular melanin transport inhibition test

[0140] Test objects: samples of Examples 1-15 and Comparative Examples 1-6.

[0141] Test method:

[0142] 3.1 Sample preparation

[0143] The sample composition was sterilized by filtration and prepared at a ratio of 0.45 mg sample to 1 ml cell culture medium;

[0144] 3.2 Cell culture

[0145] Mouse B16 melanoma cells were grown to a confluent state, digested with trypsin-EDTA for 5 minutes, and then dispersed by blowing. The cell suspension was aspirated into a centrifuge tube and centrifuged at 1000 r / min for 5 minutes. The supernatant was discarded and the culture medium was added to obtain a cell suspension. The cell suspension was diluted to a certain concentration with the culture medium. Cells in the logarithmic growth phase were inoculated in a 6-well plate at a cell density of 1×10 4 cfu / ml, 2 mL per well, mark the top cover of the cell plate after laying the plate, and culture in the incubator for 24 h.

[0146] After the cells were cultured for 24 hours, they were divided into a sample group and a blank group. The cell culture medium of the sample group was replaced with a culture medium containing the test sample, and the blank group was added with culture medium, 2 mL per well, and cultured in a 37°C, 5% CO2 incubator for 48 hours; after 48 hours, the culture medium was replaced, 3 ml of culture medium containing the sample was added to the sample group, and the blank group was added with culture medium, and cultured in a 37°C, 5% CO2 incubator for 72 hours; after 72 hours of culture, the culture medium was replaced, 3 ml of culture medium containing the sample was added to the sample group, and the blank group was added with culture medium, and cultured in a 37°C, 5% CO2 incubator for another 48 hours.

[0147] 3.3 Detection of extracellular melanin content

[0148] After the cell culture in 3.2 is completed, the culture medium in the culture medium is sucked out as much as possible into the centrifuge tube, and 200 μL of melanin extract is added to each tube, shaken evenly, and heated in an 80°C water bath for 1 hour. After cooling, moderately centrifuge the tube wall droplets, blow and beat evenly, and suck 150 μL of the solution from each centrifuge tube, transfer to a 96-well plate, and detect the absorbance of each well at 405 nm with an ELISA reader.

[0149] Extracellular melanin transport inhibition rate (%) = (1-average absorbance value of sample / average absorbance value of blank) × 100%. The results are shown in Table 1.

[0150] Table 1

[0151]

[0152]

[0153] As can be seen from Table 1 and the data of Examples 1-3, the skin care composition with whitening effect provided by the present invention has an intracellular tyrosinase inhibition rate of more than 37%, an intracellular melanin inhibition rate of more than 26%, and an extracellular melanin transport inhibition rate of more than 21%, indicating that the composition provided by the present application has a significant whitening effect on human skin.

[0154] It can be seen from Examples 1, 4 and 8 that when microbial derivatives and polypeptides are added to the composition provided by the present invention, the composition can better inhibit the formation of skin melanin, and there is a synergistic effect between the two.

[0155] It can be seen from Example 1 and Examples 5-7, 9-10 that the selection of the components of the vitamin derivatives and polypeptides also has a certain influence on the whitening effect of the composition.

[0156] It can be seen from Examples 1 and 11-15 that the synergistic combination of white truffle extract, black truffle extract, glabridin, galactoglobulus extract and α-arbutin in the plant composition can make the whitening effect of the composition better. The lack of any one component will reduce the whitening effect of the composition.

[0157] From the comparison between Example 1 and Comparative Examples 1-6, it can be seen that when the composition is not the specific whitening mechanism combination of the present application, the intracellular tyrosinase inhibition rate, the intracellular melanin inhibition rate, and the extracellular melanin transport inhibition rate of the sample will all be significantly reduced, indicating that the plant extract, nicotinamide adenine dinucleotide, tetrahydrocurcumin, trehalose, nicotinamide and phenethyl resorcinol have a synergistic effect in whitening effect.

[0158] Test Example 4: Safety Evaluation Experiment

[0159] This project evaluates irritation through the red blood cell hemolysis test. The red blood cell hemolysis test is one of the alternative methods to the rabbit eye irritation test (Draize test). The basic principle is to evaluate the damage of chemicals to eye tissue cells by measuring the amount of hemoglobin dissolution and the degree of denaturation. Internationally, the RBC test is mainly used to evaluate the eye irritation of chemicals such as cosmetics and raw materials. The classification standards are shown in the following table.

[0160]

[0161]

[0162] The results of the red blood cell hemolysis experiment on the samples of Examples 1-15 are shown in Table 2, where L / D is the ratio of HD50 (the sample concentration when 50% of the red blood cells are hemolyzed) to DI (protein denaturation index).

[0163] Table 2

[0164]

[0165]

[0166] It can be seen from Table 2 that the skin care composition of the present invention is mild and non-irritating, and has high safety in use.

[0167] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate a skin care composition with whitening effect and its application, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary ingredients, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0168] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0169] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A skin care composition with whitening effect, characterized in that: The skin care composition comprises a plant extract, nicotinamide adenine dinucleotide, tetrahydrocurcumin, trehalose, nicotinamide and phenethylresorcinol; The plant extract comprises any one of white truffle extract, black truffle extract, galactotrophic algae extract, glabridin or α-arbutin, or a combination of at least two thereof.

2. The skin care composition with whitening effect according to claim 1, characterized in that: The plant extract comprises a combination of white truffle extract, black truffle extract, glabridin, globulus extract and α-arbutin; Preferably, the mass ratio of the white truffle extract, the black truffle extract, the glabridin, the galactotrophic algae extract and α-arbutin is (1-2):(0.5-1.5):(1-3):(0.1-1):(1-2); Preferably, the Chlorella extract comprises Chlorella exosomes and / or Haematococcus pluvialis extract.

3. The skin care composition with whitening effect according to claim 1 or 2, characterized in that: The skin care composition also includes a vitamin derivative; Preferably, the vitamin derivative comprises any one of 3-o-ethyl ascorbic acid, ascorbyl tetraisopalmitate or tranexamic acid, or a combination of at least two thereof.

4. The skin care composition with whitening effect as claimed in claim 3, characterized in that: The vitamin derivative is a combination of 3-o-ethyl ascorbic acid, ascorbyl tetraisopalmitate and tranexamic acid; Preferably, the mass ratio of 3-o-ethyl ascorbic acid, ascorbyl tetraisopalmitate and tranexamic acid is (0.1-2):(1-3):(0.5-1.5).

5. The skin care composition with whitening effect according to any one of claims 1 to 4, characterized in that: The skin care composition with whitening effect also includes a polypeptide; Preferably, the polypeptide comprises carnosine and / or glutathione.

6. The skin care composition with whitening effect according to claim 5, characterized in that: The polypeptides include carnosine and glutathione; Preferably, the mass ratio of carnosine to glutathione is 1:(2-3).

7. The skin care composition with whitening effect according to any one of claims 1 to 6, characterized in that: The skin care composition with whitening effect comprises plant extracts, nicotinamide adenine dinucleotide, tetrahydrocurcumin, trehalose, nicotinamide, phenethyl resorcinol, vitamin derivatives and polypeptides; Preferably, the skin care composition with whitening effect comprises, by weight, 4-10 parts of plant extracts, 1-3 parts of nicotinamide adenine dinucleotide, 2-4 parts of tetrahydrocurcumin, 4-8 parts of trehalose, 2-5 parts of nicotinamide, 0.1-2 parts of phenethylresorcinol, 1-2 parts of vitamin derivatives and 1-3 parts of polypeptides.

8. Use of the skin care composition with whitening effect as claimed in any one of claims 1 to 7 in the preparation of cosmetics.

9. The use according to claim 8, characterized in that The mass percentage of the composition with whitening effect in the cosmetic is 1-20%; Preferably, the cosmetics include any one of essence, essence cream, lotion, facial mask or gel.

10. A lotion, characterized in that: The raw material composition of the lotion comprises the skin care composition with whitening effect as described in any one of claims 1 to 7 and water; Preferably, the raw material composition of the lotion further includes additives, and the additives include any one of moisturizers, emulsifiers, preservatives, fragrances or thickeners, or a combination of at least two of them.

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