179 results about "Arbutin" patented technology

The invention provides anti-aging collagen peptide mask liquid and a preparation method thereof. The mask liquid is prepared from the following components in percentage by mass: 1 to 2 percent of tremella polysaccharide, 3 to 4 percent of herba portulacae extract, 2 to 3 percent of radix gentianae extract, 0.3 to 0.8 percent of nicotinamide, 1.2 to 1.6 percent of alpha-arbutin, 0.2 to 0.6 percent of chrysanthellum indicum extract, 0.7 to 1.5 percent of geranium flower / leaf / stem extract, 0.4 to 0.8 percent of aloe barbadensis extract, 0.7 to 1.3 percent of radix sophorae flavescentis extract, 0.3 to 0.9 percent of herba houttuyniae extract, 1.2 to 1.7 percent of herba centellae extract and the balance of water. The traditional Chinese medicine composition is prepared from the following raw materials in percentage by weight: 0.2 to 0.6 percent of fructus hippophae extract, 0.02 to 0.06 percent of peony root bark extract, 0.06 to 0.09 percent of scutellaria baicalensis root extract, 0.04 to 0.08 percent of rosemary extract, 0.01 to 0.04 percent of pearl powder and the like. The mask liquid prepared by the invention has an anti-aging effect.

The invention discloses an arbutin derivative 6-O-Caffeoylbutin as well as a preparation method and application of the arbutin derivative 6-O-Caffeoylbutin. The structural formula of a 6-O-Caffeoylbutin compound is shown as a formula (I): # imgabs0. The invention also discloses application of the arbutin derivative (6-O-Caffeoylbutin) disclosed by the invention in preparation of an antioxidant preparation. According to the invention, the 6-O-Caffeoylbutin extracted for the first time shows excellent antioxidant activity, the ability of the 6-O-Caffeoylbutin to remove ABTB < + > free radicals is stronger than that of ascorbic acid and beta-arbutin, and a material basis is provided for development of novel antioxidants.

The invention provides anti-aging moisturizing essence and a preparation method thereof, and belongs to the technical field of cosmetics. The essence is prepared from a plant extract, sodium pyrrolidone carboxylate, hexapeptide, nicotinamide mononucleotide, vitamin B5, galactose yeast fermentation product filtrate, b-glucan, sodium hyaluronate, glycerol, tranexamic acid, blue copper peptide, arbutin, nicotinamide, vitrin, a bifidus yeast fermentation product, a bifidus yeast fermentation lysate and the like. Wherein the plant extract is prepared by mixing rosemary, frankincense resin, tea flowers, sandalwood and jasmine flowers through ultrasonic extraction, comprehensive skin care is achieved from multiple angles of anti-aging, whitening, moisturizing and repairing, the overall health of the skin is guaranteed, and the skin care cream is suitable for various skin types.

The invention provides an alpha-glucosidase mutant and application thereof in synthesis of alpha-arbutin, and the alpha-glucosidase mutant comprises the following steps: maltose and hydroquinone are taken as substrates, the recombinant bacteria are taken as a catalyst, catalytic reaction is carried out under a heating condition, and the alpha-arbutin is obtained. According to the synthesis method, maltose and hydroquinone are used as reaction substrates, and alpha-arbutin is generated through whole-cell catalysis; meanwhile, the sequence of the alpha-glucosidase mutant is integrated onto a pRSEDuet-1 expression vector and transferred into escherichia coli BL21 (DE3) for expression, a reaction system of recombinant bacteria is optimized, the optimal catalytic condition is determined, the yield of alpha-arbutin under the optimal condition is 24.8 g / L, the yield can reach 95.6% and is improved by 1.2 times compared with that of an original strain, and the alpha-glucosidase mutant has a good application prospect.

The present invention relates to the technical field of cosmetics, and in particular to a hair dye composition for preventing hair breakage, hypoallergenic and repairing, and a preparation method and application thereof. The composition is composed of agent A and agent B in a mass ratio of 1:0.8-1.5. The raw materials of agent A are hydrogen peroxide, glycerol, thickener, cetearyl alcohol polyether, sodium stannate, hydroxyethylidene diphosphoric acid and water. The raw materials of agent B are: p-phenylenediamine, sodium sulfite, triethanolamine, urea, Polygonum multiflorum extract, Acorus calamus extract, Nepeta tenuifolia extract, chelating agent, ascorbate, preservative, keratin, trehalose, 4-amino-2-hydroxytoluene, polyquaternium salt, squalane, arbutin, allantoin, bisabolol, emulsifier, polyglutamic acid, octadecyl alcohol, wheat germ oil and water. The hair dye of the present invention has a good hair dyeing effect, and has hypoallergenicity, and also has a good effect of preventing hair breakage and repairing hair quality.

The invention discloses external emulsifiable paste for directionally delivering tranexamic acid to an epidermal layer, which is prepared from the following raw materials in percentage by weight: 1-15% of tranexamic acid, 0.5-7% of alpha-arbutin, 0.3-4% of edetate disodium, 1-5% of azone, 1-5% of sodium dodecyl benzene sulfonate and the balance of water. The skin care product is prepared from, by weight, 0.1%-2% of salicylic acid, 1%-10% of glycerin, 2%-15% of propylene glycol, 0.1%-4% of tween-80 or 0.4%-2% of sorbitan oleate, 0.5%-5% of alpha-tocopherol, 0.5%-5% of diisostearyl alcohol malate, 0.5%-6% of soybean phospholipid, 1%-10% of glycerin monostearate, 0.5%-10% of tetradecanol, 0.02%-0.1% of propyl hydroxybenzoate, 1%-10% of stearic acid, 0.5%-6% of stearyl alcohol and the balance water. Meanwhile, the invention also discloses a preparation method, experiments show that the external cream prepared by the invention has strong capacity of delivering tranexamic acid to the epidermal layer and can ensure that the tranexamic acid can be quickly absorbed by cells to take effect, and the defect that tranexamic acid is difficult to pass through skin cuticle obstacles due to strong hydrophilicity is overcome.

The invention discloses application of a composition containing ginsenoside Rg5 in preparation of whitening, freckle removing and oxidation resisting products, and belongs to the technical field of biology. By combining rare ginsenoside Rg5 with alpha-arbutin, it is found that the composition can effectively resist oxidation, prevent, relieve or treat melanin deposition and inhibit melanin generation, and the effects of whitening and removing freckles are achieved.

The invention relates to a skin care composition with a whitening effect and application of the skin care composition. The skin care composition is prepared from a plant extract, nicotinamide adenine dinucleotide, tetrahydrocurcumin, trehalose, nicotinamide and phenylethyl resorcinol, the plant extract comprises any one or a combination of at least two of a white truffle extract, a perigord truffle extract, a chlorella extract, glabridin or alpha-arbutin. The plant extract, the nicotinamide adenine dinucleotide, the tetrahydrocurcumin, the trehalose, the nicotinamide and the phenethyl resorcinol are creatively combined and matched for use, a synergistic interaction effect is achieved in the whitening aspect, and the composition is mild in performance, free of irritation, safe and free of toxic and side effects and has a good whitening effect after being applied to cosmetic products. The skin whitening effect can be obviously improved, and the good use feeling is achieved.

The invention relates to the technical field of enzyme catalysis, in particular to sucrose phosphorylase with enhanced heat resistance and application of the sucrose phosphorylase in high-temperature catalytic preparation of alpha-arbutin, the amino acid sequence of the sucrose phosphorylase is shown as SEQ ID NO: 3, and compared with wild type sucrose phosphorylase, the sucrose phosphorylase has higher heat resistance. According to the sucrose phosphorylase with enhanced heat resistance, industrial biological catalysis at high temperature is realized, the catalytic efficiency can be improved, the risk of microbial pollution is reduced, meanwhile, the viscosity of a substrate is reduced, the transfer speed is increased, and the sucrose phosphorylase has important significance in shortening the reaction time and improving the production efficiency.

The invention discloses a whitening and soothing eurotium cristatum tea fermentation composition as well as a preparation method and application thereof, and relates to the technical field of skin products. The invention provides a whitening and soothing eurotium cristatum tea fermentation composition. The whitening and soothing eurotium cristatum tea fermentation composition is prepared from the following components in parts by weight: 0.5 to 2 parts of eurotium cristatum tea fermentation filtrate, 0.1 to 0.8 part of alpha-arbutin and 0.01 to 1.5 parts of rhizoma gastrodiae root extract. When the eurotium cristatum tea fermentation filtrate, the gastrodia elata root extract and the alpha-arbutin are cooperatively used, the limitation of a single effect can be broken through, and the effects of safely whitening, quickly relieving and persistently shrinking pores are considered.

The invention belongs to the technical field of cosmetics, and particularly relates to a silk fibroin aggregate-based dispersed organic active substance as well as a preparation method and application thereof. The preparation method of the silk fibroin aggregate-based dispersed organic active substance comprises the following steps: (1) dissolving an organic active substance in a first solvent to obtain a first solution; dissolving the silk fibroin aggregate in a second solvent to obtain a second solution; (2) slowly adding the second solution into the first solution, mixing and homogenizing to obtain a silk fibroin aggregate-based dispersed organic active substance; wherein the silk fibroin aggregate is prepared by mixing a silk fibroin aqueous solution and alcohol. The silk fibroin aggregate-based dispersed organic active substance prepared by the preparation method can improve the bioavailability of the organic active substance (such as vitamin C, hyaluronic acid, coenzyme Q10, nicotinamide, arbutin, kojic acid, licorice extract, centella extract, chamomile extract or green tea extract) in cosmetics.

The invention provides a whitening and anti-aging extracellular vesicle compound as well as a preparation method and application thereof. The extracellular vesicle compound comprises extracellular vesicles derived from mesenchymal stem cells and active ingredients loaded in the extracellular vesicles, the active component comprises any one or a combination of at least two of retinoic acid, arbutin, panthenol, nicotinamide, ectoine, vitamine, hyaluronic acid, vitamin C, resveratrol or retinol; the ratio of the extracellular vesicles to the active ingredients is (1-10) * 10 < 8 >: (0.01-1) mg. The skin-care active ingredients are entrapped in the extracellular vesicles derived from the mesenchymal stem cells, so that the delivery efficiency of the skin-care active ingredients can be remarkably improved, the direct irritation of free components to the skin can be reduced, the generation of matrix metalloproteinase-1 (MMP-1) and melanin can be effectively inhibited, the light aging process of the skin can be delayed, and the skin-care composition has excellent whitening and anti-aging effects.

The application discloses application of a complex containing Dendrobium officinale leaf extract in preparation of a whitening and freckle-removing preparation, and the complex comprises the Dendrobium officinale leaf extract, a Rhizoma Polygonati extract and beta arbutin. After the components of the complex are mixed in a specific ratio and fully combined and synergized, the ability of inhibiting tyrosinase activity can be significantly improved by 300%, 175% and 87% respectively compared with the Dendrobium officinale leaf, the Rhizoma Polygonati and the beta arbutin, the melanin generation in zebra fish within 72 hours can be effectively inhibited, the melanin content can be obviously reduced, the melanin inhibition rate is increased by 394%, 532%, 24.14% and 37.39% respectively compared with the Dendrobium officinale leaf group, the Rhizoma Polygonati group, the beta arbutin group and the Dendrobium officinale leaf-Rhizoma Polygonati group, the Dendrobium officinale leaf-Rhizoma Polygonati complex can obviously improve the melanin inhibition rate in zebra fish, the combination of the two and the beta arbutin can make the melanin inhibition rate reach 98.70%, and the whitening and freckle-removing effect of skin can be greatly enhanced.

The invention belongs to the technical field of cosmetics, and particularly relates to whitening and freckle-removing essence oil and a preparation method thereof. According to the invention, adamantane formyl is introduced into an alpha-arbutin benzene ring to obtain the adamantane formyl modified alpha-arbutin derivative, so that the compatibility of alpha-arbutin and base oil is enhanced; the alpha-arbutin is prevented from being converted into hydroquinone by utilizing the large steric effect of adamantane formyl, so that the safety of the whitening and freckle-removing essence oil is improved; the tranexamic acid is wrapped by the ceramide nano-emulsion, so that the skin allergy risk caused by ingestion of the tranexamic acid is reduced; the ceramide nano-emulsion and tranexamic acid act together to repair skin collagenous fibers, block melanin transfer and inhibit melanin synthesis; trilinolein and glabridin are catalyzed by lipase to form a compound, so that the molecular compatibility and photo-thermal stability of glabridin are enhanced; glycerin promotes transdermal absorption of glabridin and enhances the efficacy of the whitening and freckle-removing essence oil.

The present invention relates to an anti-allergy, whitening and freckle-removing composition and a preparation method thereof. The anti-allergy, whitening and freckle-removing composition is composed of the following components in mass fractions: 0.5-2% of Kalanchoe extract, 0.5-2% of Cornus controversa Hance extract, 0.5-2% of Yucca filamentosa extract, 0.5-2% of Chrysanthemum cinerariifolium extract, 1-3% of VC ethyl ether, 0.5-5% of arbutin, 0.1-2% of 4-butylresorcinol, 1-10% of niacinamide, 3-15% of humectant, 3-15% of emollient, 0.5-2% of penetrant, and the balance being water. The anti-allergy, whitening and freckle-removing composition is added with Kalanchoe extract, Cornus controversa Hance extract, Yucca filamentosa extract and Chrysanthemum cinerariifolium extract. Through the synergistic effect of the four extracts, the anti-allergy, whitening and freckle-removing composition has little irritation while maintaining excellent whitening and freckle-removing effects.

The invention discloses a method for synthesizing alpha-arbutin through biotransformation, and belongs to the technical field of biochemistry. A gene cloning technology is utilized, sucrose phosphorylase Spase in leuconostoc mesenteroides and sucrose phosphorylase gtfa in bifidobacterium adolescentis are screened and found through a Deep Molecules and DLKcat algorithm on a Uniprot database, novopro is utilized for codon optimization, then the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa are expressed on a plasmid vector PET-28a and are converted into an escherichia coli BL21 (DE3) strain, and the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa in the bifidobacterium adolescentis are obtained. An engineering strain for heterologous expression of the gene is constructed by taking the gene as a chassis cell. According to the process, sucrose and hydroquinone are taken as co-substrates, and bioconversion is carried out under the action of sucrose phosphorylase from different sources to obtain alpha-arbutin.

The application discloses a preparation method of arbutin analogues with high transdermal absorption efficiency, and is characterized in that the method comprises the following steps: under the action of a solvent and a catalyst, compound I and compound II are subjected to esterification to obtain compound III, wherein the compound III is the arbutin analogue. The arbutin (compound I) is used as a raw material, the structure of the arbutin is modified through esterification, the transdermal performance of the arbutin is improved, the bioavailability is increased, and finally the whitening effect is improved.

The invention relates to the technical field of cosmetics, and discloses a herbal probiotic composition with whitening and anti-aging effects and a preparation method thereof. The herbal probiotic composition is prepared from alpha-arbutin, licoflavone, bakuchiol, resveratrol, ellagic acid, a metagen complex, skin micro-ecological prebiotics, sodium phytate, ferulic acid, rosemary polyphenol, polycystic liposome and the balance of a carrier suitable for skin. The herbal probiotic composition disclosed by the invention realizes stable co-delivery of hydrophilic and lipophilic activities and effective retention of cuticles under mild pH, synergistically inhibits blackness, resists oxidation and supports microecology, and improves the effective content and batch-to-batch consistency in the shelf life through metal ion accessibility control, so that the whitening and anti-aging effects are improved.

The invention provides lignan, and belongs to the technical field of medicines. According to the invention, a compound shown as a formula I and a compound shown as a formula II are extracted, separated and identified from schisandra chinensis oil for the first time, and the compound shown as the formula I and the compound shown as the formula II are superior to a positive drug group arbutin in the aspect of inhibiting generation of melanin of mouse skin melanoma cells (B16F10); an inflammatory factor release inhibition experiment research shows that the compound shown in the formula I and the compound shown in the formula II have relatively good activity in the aspect of inhibiting LPS-induced mouse mononuclear macrophage RAW264.7 from releasing IL-6 and TNF-alpha; in a skin irritation experiment, the schisandra chinensis non-saponifiable matter containing the compound shown in the formula I and the compound shown in the formula II is non-irritant to the skin of a white rabbit, and it is proved that the compound shown in the formula I and the compound shown in the formula II can be applied to preparation of whitening and anti-inflammatory products. Formula I; formula II.

The application discloses a mycobacterium maltose transferase Co GlgE and an encoding gene and application thereof.The maltose transferase Co The amino acid sequence of the GlgE is shown as SEQ ID NO.1.The application discloses a maltose transferase Corallococcus derived from mycobacterium sp.EGB Co GlgE, which can disperse maltoligosaccharide with a polymerization degree (DP) X into products with a DP of X ± 2n (X ≥ 3). Co The GlgE has glycosylation modification effects on alpha-arbutin and beta-arbutin, the biological activity of the modified arbutin is significantly improved, and the application value of the arbutin as an additive of whitening skin care products is improved.

The invention discloses a quantum chemical screening method for extracting arbutin, and relates to the technical field of natural product extraction, and the quantum chemical screening method comprises the following steps: constructing an arbutin single-molecule model and a hydrogen bond donor and hydrogen bond receptor single-molecule model by using GaussView software, firstly performing conformation search through MOPAC and molplus programs to obtain a most stable molecular structure, and then extracting the arbutin from the molecular structure; carrying out geometric optimization and single-point energy calculation by utilizing Gaussian; the optimized hydrogen bond donor and hydrogen bond acceptor establish random clusters according to different molar ratios, the random clusters with target molecules are established, a cluster model with an optimal structure is selected for geometric optimization and single-point energy calculation, meanwhile, the binding energy between the eutectic solvent and arbutin is calculated, and IGMH analysis, ESP analysis and AIM analysis are carried out. The invention provides a universal method for green and efficient extraction of active ingredients of natural products.

The present invention provides an α-glucosidase mutant and its application in the synthesis of α-arbutin, including the following steps: using maltose and hydroquinone as substrates, and using the recombinant bacterium as a catalyst, carrying out a catalytic reaction under heating conditions to obtain the α-arbutin. The synthesis method of the present invention uses maltose and hydroquinone as reaction substrates and uses whole-cell catalysis to generate α-arbutin; at the same time, the sequence of the α-glucosidase mutant is integrated into the pRSEDuet-1 expression vector, transferred into Escherichia coli BL21(DE3) for expression, and the reaction system of the recombinant bacterium is optimized to determine the optimal catalytic conditions. Under the optimal conditions, the yield of α-arbutin is 24.8 g / L, and the yield can reach 95.6%, which is 1.2 times higher than that of the original strain, and has good application prospects.

The invention relates to the technical field of microbial fermentation, and discloses a fermentation composition with a whitening effect and a preparation method and application thereof. According to the application, Chinese rose, a sodium glutamate culture medium and sprouted mung beans are respectively used as fermentation objects, and are fermented by selecting plant lactobacillus and bacillus subtilis to respectively obtain a Chinese rose fermented product, a glutamic acid fermented product and a mung bean sprout fermented product; compared with the prior art, the arbutin fermented product has the advantages that the arbutin fermented product shows more excellent monophenol enzyme inhibition rate and diphenol enzyme inhibition rate which are superior to those of arbutin, particularly, the effect is optimal when the three fermented products are compounded, and a theoretical basis is provided for development and application of whitening cosmetic raw materials and products.

The invention discloses an amylosucrase mutant and application thereof in synthesis of alpha-arbutin, the mutant is obtained by mutation of an amino acid sequence of amylosucrase derived from coprophilobacterium mediterranei, and the amino acid sequence of the mutant is shown as SEQ ID NO.4. The invention further discloses a preparation method of the amylosucrase mutant. Compared with a wild strain, the catalytic activity, the temperature stability and the substrate tolerance of the amylosucrase mutant disclosed by the invention are remarkably improved; the recombinant bacteria constructed by using the recombinant bacteria can be used as a whole-cell catalyst to efficiently catalyze a substrate to synthesize alpha-arbutin in a reaction system containing hydroquinone. Therefore, the amylosucrase mutant disclosed by the invention has a good application prospect.

The invention relates to the technical field of beta-arbutin extraction, and discloses a method for extracting beta-arbutin from beta-arbutin fermentation liquor. The method comprises the steps of separating and removing mycoprotein, passing through an ultrafiltration membrane, passing through a nanofiltration membrane, passing through a reverse osmosis membrane, decolorizing, concentrating and crystallizing. The method can effectively improve the impurity removal efficiency of the fermentation liquor, shorten the process time, improve the product content and the product yield, reduce the energy consumption, reduce the cost, and reduce the discharge and treatment difficulty of wastewater. In addition, membrane concentration process conditions are mild, the product decomposition rate is low, and the extraction income is greatly improved.

The invention belongs to the technical field of biology, and particularly provides an arbutin synthetase mutant and application thereof. The arbutin synthetase mutant is obtained through rational design and enzyme evolution screening on the basis of an amino acid sequence as shown in SEQ ID NO: 1, and compared with wild arbutin synthetase, the arbutin synthetase mutant is changed in protein structure and function. The catalytic activity of the arbutin synthetase mutant provided by the invention is obviously improved, and higher conversion rate and conversion rate from arbutin synthetase to beta-arbutin can be obtained. A shikimic acid pathway is enhanced in escherichia coli, and an arbutin synthetase mutant gene is introduced to construct a high-yield beta-arbutin producing strain. The method for producing beta-arbutin has the characteristics of simplicity in operation, low cost, high product synthesis efficiency and reduction of environmental pollution, and has a good industrialization prospect.

The invention provides a composition containing bioactive peptide, which is prepared from the following components in parts by weight: 3 to 6 parts of bioactive peptide, 0.2 to 0.6 part of hyaluronic acid, 1 to 3 parts of witch hazel, 0.1 to 0.5 part of arbutin and 100 to 150 parts of water. The feed also comprises 6-12 parts of a plant extract mixed solution and 0.5-1.5 parts of a vitamin mixture; the bioactive peptide is a bioactive peptide extracted from fish; the plant extraction mixed solution is a plant extraction solution of a mixture of aloe, grape seeds, cassia seeds and mint in a mass ratio of 1: (2-3): (1.5-2.5): (3-4); under the synergistic effect of all the components, the wrinkle removal, skin care and other properties of the composition can be obviously improved, and when the composition is applied to eye care essence, the eye skin care requirements can be better met.

The present invention relates to the fingerprint spectrum of Dapanlongqi medicinal material and the application of multi-component analysis in its quality control. The HPLC fingerprint spectrum of the Dapanlongqi medicinal material is basically consistent with Figure 1, with 14 characteristic fingerprint chromatographic peaks, wherein peak 1 is arbutin, peak 2 is gallic acid, peak 3 is bergenin, peak 5 is catechin, peak 7 is 4-O-galloyl bergenin, peak 9 is 11-O-galloyl bergenin, and peak 12 is catechin gallate.

The invention relates to a difunctional gastrodia elata glycosyl transferase GaUGT015 gene and application thereof in preparation of gastrodin and arbutin, and belongs to the technical field of biology. The nucleotide sequence of the difunctional glycosyl transferase GaUGT015 gene of the gastrodia elata is shown as SEQ ID NO.1, and the total length of the sequence is 1407 bp; the amino acid sequence of the encoded protein is as shown in SEQ ID NO.2, and 469 amino acid residues are encoded. The gastrodia elata bifunctional glycosyl transferase GaUGT015 gene disclosed by the invention can be used as a biosynthesis regulation gene of gastrodin and arbutin, is applied to preparation of gastrodin and arbutin, and is remarkable in application prospect and easy to popularize and apply.

The invention discloses a whitening and spot-fading composition based on a fermented jasmine flower extract. The whitening and spot-fading composition comprises the following components in parts by mass: 0.1-1 part of a nano emulsifier, 0.5-8 parts of the fermented jasmine flower extract, 0.5-1.5 parts of arbutin and 1-3 parts of tranexamic acid. The nano emulsifier is a mixture of hydrogenated lecithin and phytosterol, the mass fraction ratio of arbutin to tranexamic acid is 0.5: 1, and the fermented jasmine flower extract specifically comprises a jasmine flower extract, a lactobacillus fermentation extract, water, 1, 2-hexanediol and 1, 2-pentanediol. The average particle size of the emulsion is about 256.56 nm through a nano emulsification technology, and compared with a conventional system, the transdermal absorption efficiency of active ingredients such as the jasmine flower extract, arbutin and tranexamic acid can be greatly improved, so that the active ingredients can reach action targets more easily; the problem of non-uniform dispersion caused by component solubility difference can be solved, the active component analysis risk is remarkably reduced, the system stability is improved, and the whitening and spot fading effects are enhanced.