61 results about "Arbutin" patented technology

The application discloses application of a complex containing Dendrobium officinale leaf extract in preparation of a whitening and freckle-removing preparation, and the complex comprises the Dendrobium officinale leaf extract, a Rhizoma Polygonati extract and beta arbutin. After the components of the complex are mixed in a specific ratio and fully combined and synergized, the ability of inhibiting tyrosinase activity can be significantly improved by 300%, 175% and 87% respectively compared with the Dendrobium officinale leaf, the Rhizoma Polygonati and the beta arbutin, the melanin generation in zebra fish within 72 hours can be effectively inhibited, the melanin content can be obviously reduced, the melanin inhibition rate is increased by 394%, 532%, 24.14% and 37.39% respectively compared with the Dendrobium officinale leaf group, the Rhizoma Polygonati group, the beta arbutin group and the Dendrobium officinale leaf-Rhizoma Polygonati group, the Dendrobium officinale leaf-Rhizoma Polygonati complex can obviously improve the melanin inhibition rate in zebra fish, the combination of the two and the beta arbutin can make the melanin inhibition rate reach 98.70%, and the whitening and freckle-removing effect of skin can be greatly enhanced.

The invention discloses a method for synthesizing alpha-arbutin through biotransformation, and belongs to the technical field of biochemistry. A gene cloning technology is utilized, sucrose phosphorylase Spase in leuconostoc mesenteroides and sucrose phosphorylase gtfa in bifidobacterium adolescentis are screened and found through a Deep Molecules and DLKcat algorithm on a Uniprot database, novopro is utilized for codon optimization, then the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa are expressed on a plasmid vector PET-28a and are converted into an escherichia coli BL21 (DE3) strain, and the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa in the bifidobacterium adolescentis are obtained. An engineering strain for heterologous expression of the gene is constructed by taking the gene as a chassis cell. According to the process, sucrose and hydroquinone are taken as co-substrates, and bioconversion is carried out under the action of sucrose phosphorylase from different sources to obtain alpha-arbutin.

The application discloses a preparation method of arbutin analogues with high transdermal absorption efficiency, and is characterized in that the method comprises the following steps: under the action of a solvent and a catalyst, compound I and compound II are subjected to esterification to obtain compound III, wherein the compound III is the arbutin analogue. The arbutin (compound I) is used as a raw material, the structure of the arbutin is modified through esterification, the transdermal performance of the arbutin is improved, the bioavailability is increased, and finally the whitening effect is improved.

The invention relates to the technical field of cosmetics, and discloses a herbal probiotic composition with whitening and anti-aging effects and a preparation method thereof. The herbal probiotic composition is prepared from alpha-arbutin, licoflavone, bakuchiol, resveratrol, ellagic acid, a metagen complex, skin micro-ecological prebiotics, sodium phytate, ferulic acid, rosemary polyphenol, polycystic liposome and the balance of a carrier suitable for skin. The herbal probiotic composition disclosed by the invention realizes stable co-delivery of hydrophilic and lipophilic activities and effective retention of cuticles under mild pH, synergistically inhibits blackness, resists oxidation and supports microecology, and improves the effective content and batch-to-batch consistency in the shelf life through metal ion accessibility control, so that the whitening and anti-aging effects are improved.

The application discloses a mycobacterium maltose transferase Co GlgE and an encoding gene and application thereof.The maltose transferase Co The amino acid sequence of the GlgE is shown as SEQ ID NO.1.The application discloses a maltose transferase Corallococcus derived from mycobacterium sp.EGB Co GlgE, which can disperse maltoligosaccharide with a polymerization degree (DP) X into products with a DP of X ± 2n (X ≥ 3). Co The GlgE has glycosylation modification effects on alpha-arbutin and beta-arbutin, the biological activity of the modified arbutin is significantly improved, and the application value of the arbutin as an additive of whitening skin care products is improved.

The invention discloses a quantum chemical screening method for extracting arbutin, and relates to the technical field of natural product extraction, and the quantum chemical screening method comprises the following steps: constructing an arbutin single-molecule model and a hydrogen bond donor and hydrogen bond receptor single-molecule model by using GaussView software, firstly performing conformation search through MOPAC and molplus programs to obtain a most stable molecular structure, and then extracting the arbutin from the molecular structure; carrying out geometric optimization and single-point energy calculation by utilizing Gaussian; the optimized hydrogen bond donor and hydrogen bond acceptor establish random clusters according to different molar ratios, the random clusters with target molecules are established, a cluster model with an optimal structure is selected for geometric optimization and single-point energy calculation, meanwhile, the binding energy between the eutectic solvent and arbutin is calculated, and IGMH analysis, ESP analysis and AIM analysis are carried out. The invention provides a universal method for green and efficient extraction of active ingredients of natural products.

The invention relates to the technical field of microbial fermentation, and discloses a fermentation composition with a whitening effect and a preparation method and application thereof. According to the application, Chinese rose, a sodium glutamate culture medium and sprouted mung beans are respectively used as fermentation objects, and are fermented by selecting plant lactobacillus and bacillus subtilis to respectively obtain a Chinese rose fermented product, a glutamic acid fermented product and a mung bean sprout fermented product; compared with the prior art, the arbutin fermented product has the advantages that the arbutin fermented product shows more excellent monophenol enzyme inhibition rate and diphenol enzyme inhibition rate which are superior to those of arbutin, particularly, the effect is optimal when the three fermented products are compounded, and a theoretical basis is provided for development and application of whitening cosmetic raw materials and products.

The invention discloses a whitening and spot-fading composition based on a fermented jasmine flower extract. The whitening and spot-fading composition comprises the following components in parts by mass: 0.1-1 part of a nano emulsifier, 0.5-8 parts of the fermented jasmine flower extract, 0.5-1.5 parts of arbutin and 1-3 parts of tranexamic acid. The nano emulsifier is a mixture of hydrogenated lecithin and phytosterol, the mass fraction ratio of arbutin to tranexamic acid is 0.5: 1, and the fermented jasmine flower extract specifically comprises a jasmine flower extract, a lactobacillus fermentation extract, water, 1, 2-hexanediol and 1, 2-pentanediol. The average particle size of the emulsion is about 256.56 nm through a nano emulsification technology, and compared with a conventional system, the transdermal absorption efficiency of active ingredients such as the jasmine flower extract, arbutin and tranexamic acid can be greatly improved, so that the active ingredients can reach action targets more easily; the problem of non-uniform dispersion caused by component solubility difference can be solved, the active component analysis risk is remarkably reduced, the system stability is improved, and the whitening and spot fading effects are enhanced.

The invention discloses a Sorosil decoction specific chromatogram and a construction method and application thereof, and belongs to the technical field of traditional Chinese medicine analysis and detection. The method comprises the following steps: respectively dissolving reference substances of bergenin, arbutin, glycyrrhizic acid, liquiritin, liquiritigenin and gallic acid in a solvent to obtain reference substance solutions; adding soroxil decoction into a solvent, carrying out ultrasonic extraction, and filtering to obtain a test solution; and injecting the reference solution and the test solution into a high performance liquid chromatograph for detection, taking the specific chromatogram of the reference solution as a reference chromatogram, selecting common peaks from the specific chromatogram of the test solution, and constructing the specific chromatogram of the soroxil decoction. The invention also discloses a Sorosil decoction characteristic spectrum and application thereof. According to the specific chromatogram of the soroxi decoction, rapid identification of the formula of the soroxi decoction can be realized, the method is simple, the research blank of the specific chromatogram in quality control of the classic famous-prescription soroxi decoction and the preparation thereof is filled up, and a powerful guarantee is provided for quality improvement of the classic famous-prescription soroxi decoction.

The invention relates to the technical field of cosmetic component detection and analysis, and discloses a method for rapidly recognizing arbutin in cosmetics based on surface enhanced Raman spectroscopy, AgNPs sol is prepared by an ascorbic acid oxidation-reduction method, and an SERS sensing interface is constructed through aspartic acid electro-polymerization modification; for different dosage forms of cosmetics, a to-be-detected solution is obtained through methanol extraction and centrifugal filtration purification, the to-be-detected solution and AgNPs sol are mixed and adsorbed, then a Raman spectrum is collected, pretreatment is conducted through combination of multivariate scatter correction and standard orthogonal transformation, characteristic wavelengths are screened through a genetic algorithm-combined interval partial least square method, and detection is achieved through combination of qualitative and quantitative models. Through cooperation of sensing interface specific modification and a data processing technology, the arbutin signal recognition capability is enhanced, matrix interference is eliminated, rapid and accurate detection of arbutin in cosmetics is realized, and the effects of high sensitivity, high specificity and simple and convenient operation are achieved.

The application discloses a preparation method and application of a nano self-assembly composition of arbutin-berberine, and comprises the following steps: dissolving berberine in methanol at 38-42 DEG C, adding an arbutin super-pure water solution at 38-42 DEG C, and stirring and keeping warm for 0.8-1.2 h to obtain a self-assembly solution; then, the self-assembly solution is subjected to vacuum rotary evaporation and freeze drying in sequence, and the nano self-assembly composition of arbutin-berberine is obtained. The traditional Chinese medicine nano self-assembly system based on arbutin and berberine is successfully constructed, the nanoparticles are spontaneously formed under mild conditions through intermolecular non-covalent interaction, the preparation process is simple and convenient, the conditions are mild, the damage of high temperature and other harsh conditions to active ingredients is effectively avoided, and the process adaptability is high.

A whitening nanocomposition, a preparation method therefor, and use thereof. The whitening nanocomposition comprises an active ingredient and a nanocarrier. The active ingredient is coated and / or adsorbed by the nanocarrier. The active ingredient comprises the following components: 1-15 parts by weight of α-arbutin, 1-15 parts by weight of nicotinamide, 1-10 parts by weight of 3-o-ethyl ascorbic acid, 0.1-1 part by weight of a Centalla asiatica extract, and 1-5 parts by weight of retinyl palmitate. The nanocarrier comprises the following components: 5-10 parts by weight of an emulsifier, 5-20 parts by weight of a polyol, 0.1-5 parts by weight of a phospholipid, and 40-60 parts by weight of water. The whitening nanocomposition has good skin permeability and retention performance and enables high-concentration enrichment, prolonged retention, and sustained release or controlled release within a target tissue, so as to be effectively taken in by target cells, thereby improving bioavailability and enhancing the whitening effect.

The invention discloses an arbutin derivative 6-O-Caffeoylbutin as well as a preparation method and application of the arbutin derivative 6-O-Caffeoylbutin. The structural formula of a 6-O-Caffeoylbutin compound is as shown in formula (I). The invention also discloses the application of the arbutin derivative (6-O-Caffeoylbutin) disclosed by the invention in preparation of an antioxidant preparation. According to the invention, the 6-O-Caffeoylbutin extracted for the first time shows excellent antioxidant activity, the ABTB free radical scavenging capacity of the 6-O-Caffeoylbutin is higher than that of ascorbic acid and beta-arbutin, and a material basis is provided for developing novel antioxidants.

The invention discloses a cosmetic composition with blue light resistance and multiple skin protection effects and application of the cosmetic composition. Comprising the following components: water, butanediol, glycerol, nicotinamide, erythritol, hexanediol, p-hydroxyacetophenone, alpha-arbutin, tranexamic acid, glycosylglycerol, carbomer, hydrolyzed collagen, a ganoderma lucidum extract, allantoin, euglena tiny polysaccharide, carnosine and 3-o-ethyl ascorbic acid. The synergistic relationship of various active ingredients in blue light-induced injury is researched, and it is found for the first time that the cosmetic composition has remarkable effects in the aspects of reducing blue light-induced oxidation pressure, relieving chromatosis, improving skin barrier stability and improving dark skin color; the cosmetic composition is used for preparing cosmetics with the effects of resisting blue light, whitening and brightening, and shielding and repairing, and can effectively resist blue light damage and improve the comprehensive state of skin.

The invention discloses a separation method of extracellular vesicles derived from non-medicinal parts of paris polyphylla and application of the extracellular vesicles in anti-inflammatory and whitening. According to the method, the extracellular vesicles are separated from stems, leaves and root hairs of non-medicinal parts of paris polyphylla by adopting double isoelectric precipitation combined with electrophoresis dialysis, and biological activity research is systematically performed on the extracellular vesicles sourced from the stems, leaves and root hairs. The cell and zebra fish model comprehensive evaluation proves that the extracellular vesicles from the two sources have obvious anti-inflammatory effects. However, in the melanin inhibition activity, the extracellular vesicles of the stems and leaves of the paris polyphylla are good in performance, and the inhibition effect of the extracellular vesicles on melanin generation in a zebra fish in-vivo model and the inhibition capacity of the extracellular vesicles on tyrosinase in vitro are obviously superior to those of a classic whitening agent arbutin. The limitation that only paris polyphylla tubers are utilized traditionally is broken through, development and efficacy verification of the paris polyphylla stem leaf and root hair extracellular vesicles are achieved for the first time, and the application prospect in the aspect of developing anti-inflammatory drugs and whitening cosmetics is wide.

This invention belongs to the field of biochemical technology, specifically disclosing a 6′- O A whole-cell biosynthesis method for caffeoyl arbutin and its analogues includes the following steps: using aromatic carboxylic acids and vinyl acetates as substrates, performing a transvinylation reaction under palladium catalyst and base conditions to prepare aromatic vinyl esters; using the prepared aromatic vinyl esters as acyl donors, ... β Arbutin, using acyl acceptors, undergoes an aromatic acylation reaction in an organic solvent with whole cells of Aspergillus oryzae as a catalyst to yield the 6′- O Caffeoyl arbutin or its analogues. This invention develops a two-step whole-cell catalytic method for the efficient and sustainable synthesis of CA and its analogues. The synthesized compounds exhibit potent inhibitory activity against mushroom tyrosinase. This invention pioneers a novel green biosynthetic pathway for tyrosinase inhibitors, demonstrating significant application potential in the food preservation and cosmetics industries.

1. Name of the designed product: Packaging bottle (WWP ARBUTIN BODY LOTION). 2. Use of the designed product: for packaging or transportation, bottle or similar container. 3. Design points of the designed product: in the perspective view. 4. Picture or photo that best indicates the design points: perspective view.

The invention relates to the technical field of skin treatment, and particularly provides a hydrogen-carrying material wound repair patch and a preparation method thereof, and the hydrogen-carrying material wound repair patch comprises berberine, snail slime freeze-dried powder, asiaticoside, hyaluronic acid, alpha-arbutin, sodium alginate, hydrogen, a calcium chloride aqueous solution and the like. A non-rare earth skeleton repairing-whitening system is constructed, wound repairing and whitening after healing are both considered, and the effects that the skin wound is repaired, and the skin color of the wound after healing is not darkened are achieved.

The application discloses a preparation method of arbutin, and the preparation method comprises the following steps: (1) C5-halogenated ester (II) is reacted with 2-mercaptobenzothiazole (MBT) to generate C5-BT (III), and C5-BT (III) is further oxidized to prepare C5-BTS (IV); and (2) C5-BTS (IV), C15-BTS (V) and C10-dialdehyde (VI) are coupled to prepare arbutin (I), and the specific route can be C5+C10→C15, C15+C15→C30, or C15+C10→C25, C25+C5→C30; the method has low raw material cost, avoids the use of phosphorus salt in a traditional synthesis route, and provides an efficient, economical and environmentally-friendly solution for large-scale clean production of arbutin.

The invention discloses an arbutin analogue, a preparation method thereof and application of the arbutin analogue in preparation of a medicine for treating chronic colitis, and belongs to the technical field of medicines. The arbutin analogue is structurally characterized in that a galactosyl group is connected with a polysubstituted benzene ring through a flexible connecting chain-(CH) n-O-to form a novel structure which is remarkably different from a known compound arbutin. The arbutin analogue is prepared through chemical synthesis, and a pharmaceutical composition containing the arbutin analogue is provided. Pharmacological experiments prove that the representative compounds hydroquinone monogalactoside and 3, 4-dihydroxyphenol galactoside show disease treatment activity obviously superior to that of arbutin in a DSS-induced mouse chronic colitis model, the disease activity index can be effectively improved, and colon tissue damage can be relieved. Therefore, the compound provided by the invention can be used for preparing medicines for preventing and / or treating chronic colitis. Formula (1)

The invention discloses a natural antibacterial agent arbutin energized self-polishing antifouling nano composite coating and a preparation method thereof.The preparation method comprises the steps that firstly, aminopropyl terminated polydimethylsiloxane and hexamethylene diisocyanate are synthesized into a prepolymer NPDI, then the prepolymer NPDI reacts with hydroxyethyl acrylate, and an organic matrix NPDA is obtained; the preparation method comprises the following steps: adsorbing arbutin by ZIF-8 to prepare ZIF-8 / Arb powder, blending the ZIF-8 / Arb powder with SiO2, adding NPDA, adding a fluorosilicone coupling agent, carrying out a blending reaction, and carrying out spraying and room temperature drying to prepare the self-polishing antifouling nano composite coating. The base body adopted by the coating is excellent in mechanical property and outstanding in structural stability, the lap joint shear strength reaches 14.08 MPa, the coating has the hydrolysis and low surface energy characteristics, and surface self-polishing and attached organism nondestructive stripping can be achieved under water flow shearing; the nano SiO2 improves wear resistance and durability, the ZIF-8 / Arb releases arbutin and Zn < 2 + > in a targeted manner in a subacid environment, synergistically and efficiently inhibits bacteria, inhibits biofilm formation, realizes long-acting active antifouling, and is suitable for a harsh marine environment.

The invention discloses an aloe barbadensis leaf water composition for removing acnes and application thereof, and the composition is prepared from the following components in percentage by mass: 91 to 97 percent of aloe barbadensis leaf water, 1 to 4 percent of nicotinamide, 1 to 4 percent of arbutin, 0.1 to 0.4 percent of benzalkonium bromide and 0.1 to 0.6 percent of chlorhexidine acetate. The invention also provides a preparation method and application of the composition. The method comprises the following steps: weighing the raw materials in proportion, then mixing the aloe barbadensis leaf water, nicotinamide, arbutin, benzalkonium bromide and chlorhexidine acetate in a container, stirring and dissolving uniformly, sampling and detecting to be qualified, disinfecting and sterilizing, and finally, sealing, packaging and storing the aloe barbadensis leaf water, the nicotinamide, the arbutin, the benzalkonium bromide and the chlorhexidine acetate. The composition is used for preparing skin care products for removing acnes. The aloe barbadensis leaf water composition for removing acnes, prepared by the preparation method disclosed by the invention, adopts acnes-removing components derived from the nature, is safe in acnes removal without hurting the skin, restores the skin to a healthy state while repairing the damaged skin, and inhibits the re-breeding of acnes and bacteria.

The present application relates to the field of cosmetic technology, and particularly relates to a low-irritation soothing plant cosmetic lotion and a preparation method thereof.The low-irritation soothing plant cosmetic lotion comprises 80-95 parts of a solvent, 0.1-10 parts of a skin conditioning agent, 0.5-10 parts of a humectant, 0.01-1 parts of a chelating agent and 0.001-1 parts of a pH regulator; the skin conditioning agent comprises an active ingredient and a cactus extract compound in a mass ratio of (40-70):1; the active ingredient comprises at least one of 377, arbutin, nicotinamide, kojic acid and glycolic acid; the cactus extract compound comprises an aggregate of cactus extract and cerium oxide particles doped with each other; and the cactus extract compound is prepared from cerium oxide and cactus extract in a mass ratio of 1:(1-50). The present application adds a specific skin conditioning agent, which can effectively reduce the irritation of active ingredients such as whitening agents and acids in the lotion to the skin.

This invention relates to the field of skincare technology and discloses a skin-penetrating essence that balances pigmentation and repairs cells. The essence comprises the following components by weight percentage: aqueous carrier 45-80%; ethanol 2-8%; propylene glycol 3-10%; tranexamic acid 1-5%; resveratrol 0.5-3%; salicylic acid 0.2-2%; arbutin 1-4%; dimethyl sulfone 0.5-4%; butylene glycol 2-6%; azelaic acid 0.5-3%; ginseng extract 0.1-2%; angelica extract 0.1-2%; oat extract 0.5-3%; Stephania tetrandra extract 0.2-1.5%; Centella asiatica extract 0.5-3%; aloe vera extract 0.5-2.5%; golden chamomile extract 0.2-2%; caramel color 0.01-0.1%; 1,2-hexanediol 0.5-2%; p-hydroxyacetophenone 0.1-1%. The mass ratio of tranexamic acid, arbutin, and azelaic acid is (1-3):(1-2):(0.5-1.5). Brightening and fading effect: The tranexamic acid, arbutin, azelaic acid, and salicylic acid in the formula work synergistically to help inhibit melanin production, accelerate keratin metabolism, improve uneven skin tone and dullness, and make the skin more radiant and even-toned.

The invention relates to the technical field of cosmetics, and discloses a tranexamic acid-containing whitening and spot-fading repairing essence and a preparation method thereof.The provided whitening and spot-fading essence is completely prepared from safe cosmetic raw materials, is free of preservatives, mild, free of irritation and good in whitening and spot-fading effect, and further has a good anti-inflammatory effect; under the synergistic effect of tranexamic acid, alpha-arbutin and lysate of a bifidus yeast fermentation product, the whitening and spot-fading component can rapidly permeate into skin to inhibit the transfer of melanosome, so that the whitening and spot-fading effects are achieved; the tranexamic acid is used for inhibiting the activity of tyrosinase, so that the formation of melanin is effectively blocked, pigmentation is reduced, the decomposition and excretion of melanin are accelerated, the skin is kept tender and soft for a long time, skin cells are activated, the oxidation resistance of the skin is improved, and the effects of whitening and fading spots are achieved; the generation of inflammatory factors is inhibited through the liquorice extract, so that the anti-inflammatory ability of the skin and the oxidation resistance of the skin are improved.

The invention provides application of arbutin in preparation of a medicine for promoting wound healing of diabetic foot ulcer, and relates to the technical field of biomedicine, in the application, through animal experiments and cell experiments, the treatment effect of external-use arbutin cream on diabetic foot ulcer healing is systematically evaluated, and the action mechanism of the external-use arbutin cream is explored. In the research, a diabetic foot ulcer rat model is constructed, drug administration treatment is carried out in groups, meanwhile, related experiments are carried out on human prepuce fibroblasts, and through multiple detection methods and statistical analysis, it is proved that the arbutin cream plays a positive role in promoting wound healing, granulation tissue formation, collagen deposition, fibroblast migration and the like.

The invention discloses deep moisturizing and hydrating gel as well as a preparation method and application of the deep moisturizing and hydrating gel. The composition comprises the following components: bifidus yeast fermentation product filtrate, human-like collagen, hydrolyzed fish collagen peptide, alpha-arbutin, crithmum maritimum callus culture filtrate, eryngium maritimum callus culture filtrate, desert rose callus culture filtrate, peony fermentation filtrate, hydrolyzed placenta (sheep) extract, leukocyte extract and soybean fermentation metabolite. The skin care product is prepared from a brown algae extract, 1, 3-butanediol, sodium hyaluronate, D-panthenol, an aloe extracting solution, allantoin, carbomer, xanthan gum, cyclopentasiloxane (and) cyclohexasiloxane, polyacrylic acid (ester) copolymer sodium (and) lecithin, a hamamelis mollis distillate, a jania rubens extract, arginine, sweet orange essential oil and the balance of water. The anti-wrinkle gel has the advantages that the anti-wrinkle gel is mild and non-irritant, multiple effects of relieving, moisturizing, tightening and anti-wrinkle are synergistic, the gel is not easy to flow, the dosage is convenient to control, no sticky feeling exists, and the experience feeling is good.

This invention relates to the field of plant exovesicles, specifically to a Bletilla striata exovesicle complex loaded with arbutin, its preparation method, and its application. The preparation method includes the following steps: preparing a supernatant of Bletilla striata exovesicles; preparing an aqueous solution of arbutin; mixing the exovesicle supernatant with the arbutin aqueous solution, performing a cyclic freeze-thaw treatment, mixing thoroughly, allowing it to stand at room temperature for 30-40 minutes, centrifuging, and obtaining the precipitate, which is the complex. The exovesicle complex and its preparation method provided by this invention can reduce the melanin content of A375 cells by inhibiting melanin production-related genes while exerting a weak inhibitory effect on cells. Moreover, the inhibitory effect of the drug-loaded exovesicles is stronger than that of arbutin monomer at the same concentration, providing a certain research basis for the development of low-dose, high-efficiency skin whitening products.

The invention provides an anti-aging composition containing glutathione microspheres and a preparation method of the anti-aging composition. The anti-aging composition is prepared from the following components in parts by mass: 0.5 to 4 parts of arbutin, 1 to 5 parts of glutathione microspheres, 0.05 to 3 parts of ergothioneine, 0.005 to 0.02 part of oligopeptide-4, 1 to 5 parts of grape fermentation extract, 0.1 to 2 parts of sodium hyaluronate and 0.01 to 0.05 part of resveratrol substances. According to the anti-aging composition containing the glutathione microspheres, the skin roughness is lower, the skin brightness improvement rate is higher, the high DPPH free radical scavenging rate is achieved, after the anti-aging composition containing the glutathione microspheres is used for a long time, the skin can be smoother and brighter, meanwhile, DPPH free radicals can be effectively removed, and the anti-aging effect is good. And the antioxidant effect is better.

This invention relates to the field of Vaccinium bracteatum tissue culture technology, specifically to a method and application for increasing the content of arbutin and 6′-O-caffeoyl arbutin in Vaccinium bracteatum tissue culture seedlings. The method includes: using 1 / 4 MS medium as the rooting medium for the Vaccinium bracteatum tissue culture seedlings, wherein the specific concentrations of CaCl2·2H2O are 165-220 mg / L; KNO3 is 0-237.5 mg / L or 712.5-950 mg / L; MgSO4·7H2O is 0-46.25 mg / L; CuSO4·5H2O is 0.0375-0.05 mg / L; and MnSO4·4H2O is 0-11.15 mg / L. This invention achieves the controlled increase of the content of active compounds CA and Arb in Vaccinium bracteatum tissue culture seedlings by changing the mineral element concentrations in the 1 / 4 MS medium. This is an effective way to obtain large quantities of high-yielding CA and Arb Vaccinium bracteatum materials.