The present invention relates to a two-dimensional preparative
chromatography instrument
system having the online enrichment function, and applications thereof. The
system comprises a
chromatography flow channel switching valve, a
chromatography column, a
solvent pump and a
detector. According to the present invention, a
trapping column collects a first-dimensional chromatography
effluent component through valve switching and mobile phase
dilution, and the trapped component is introduced into a second-dimensional preparative column through valve switching and mobile phase
elution so as to achieve two-dimensional chromatography separation; the instrument
system can perform two-dimensional separation, and the sample and the one-dimensional separation component are effectively retained and enriched through the
trapping column interface, such that the column efficiency loss is low, and the two-dimensional separation effect is ensured; and the instrument system can enlargedly perform multiple circulation separations and multidimensional separation, and the separation effect can not be decreased along with the increasing
sample volume and the increasing separation frequency. In addition, the instrument system further has the function of the concentration
recovery on the finally-separated product so as to simplify the sample
drying process, such that the degeneration of the sample during the heating
drying process is easily be avoided.