The invention relates to a disinfecting and antiseptic Chinese patent medicine and a preparation method thereof, which is formed by mixedly preparing traditional Chinese medicine radix glycyrrhiza, baikal skullcap root, ordate houttuynia, forsythia suspense, longtube ground ivy herb and additive, wherein, the additive comprises ethanol, distilled water, tween and propylene glycol; the preparation method comprising the following steps: 1), taking material; 2) crushing the forsythia suspense and the baikal skullcap root, heating and refluxing the crushed mixture through solvent, extracting the crushed mixture, and centrifugating the obtained filtrate for reservation after frozen storage; 4) mixing the above filtrate, and concentrating the filtrate to extract for reservation; 5) evenly mixing the extract obtained in the step 4) and the tween, the propylene glycol, adding the diluted ethanol to the mixture, taking supernatant fluid, that is, obtaining the disinfecting and antiseptic Chinese patent medicine. The disinfectant which is prepared according to the technical scheme has the following advantages: 1) the invention has no toxic side effect, corrosivity, pollution of the environment, and the LD50 is larger than 20g/Kg; 2) the disinfecting effect is good; 3) the effect is not changed after storing for one year.