Method for inducing female nucleus growth with heterologous frozen sperm

Abstract

A fish allogenous frozen-sperm induced gynogenesis method comprises a frozen storage method of the allogenous fish sperm, a genetic inactivation method, an induced method for the gynogenesis diploid fry and an identification method for the gynogenesis fry. The present invention establishes the method adopting the frozen-sperm of the sea bass to induce the egg gynogenesis of a plurality of sea fishes for the first time, which selects the cold-shock starting time, cold-shock temperature and the cold-shock durance time that are separately suitable for fish eggs of the strip-spotted flounder, the turbot and the canine-tooth paralichthys olivaceus to establish a micro-satellite marking identification method of the gynogenesis fry. The percentage rate adopting the present invention to acquire the gynogenesis fry of the strip-spotted flounder, the turbot and the canine-tooth paralichthys olivaceus is 7.8 percentages to 40.7 percentages, 26 percentages to 34 percentages and 10 percentages to 25 percentages respectively. The established method of the present invention is characterized in advancing, high efficiency, accuracy and reliability, which has an important application value in fish gender controlling and the production of the all-female fries and has a broad popularization and application prospect on fish cultivation and fish breeding.

Description

Technical field: [0001] The invention belongs to fish genetic breeding technology, and is a method for cultivating fish gynogenetic diploids by using heterologous frozen sperm, which can be applied to fish sex control and production of all-female seedlings. Background technique: [0002] Male and female differences in growth rate exist in many fish species. For example, tilapia males grow more than 50% faster than females, and many marine flounder females grow much faster than males, such as star flounder and Atlantic flounder. Individuals are 50-100% faster. Due to the slow growth of male individuals, the aquaculture yield and aquaculture benefits of these flounder fishes have been reduced, the aquaculture cost has been increased, and the promotion of these fish seedlings and the formation of aquaculture industry have been seriously affected. The research on nuclear development technology, the development of all-female populations, and the promotion and application of all...

AI Technical Summary

Problems solved by technology

There are no reports on gynogenesis induced by heterologous frozen sperm in fish at home and abroad