5 results about "Turbot" patented technology

The application provides a turbot compound immune enhancer, a preparation method and application thereof. The application discloses application of a composition containing a series of components in preparation of a turbot immune enhancer, and the immune enhancer can effectively improve expression of a proinflammatory related gene of the turbot, reduce a number of bacteria in a liver or an intestinal tract, enhance a bactericidal capacity of the turbot, enhance an antioxidant stress capacity of the turbot, relieve liver damage of the turbot after being infected by bacteria, and relieve tissue damage of the turbot after being infected by bacteria. The immune enhancer component is simple, environment-friendly, low in cost and ideal in effect, and provides a new scheme for improving a breeding level of marine fish and improving a survival rate.

The present application relates to the SNP molecular marker of the body length correlation under the fermented soybean meal feeding of Scophthalmus maximus and application thereof, and belongs to the technical field of molecular marker, the body length trait of the present application is analyzed by using whole genome correlation analysis on Scophthalmus maximus, the SNP molecular marker of the significant correlation under the fermented soybean meal feeding of Scophthalmus maximus and body length trait is obtained, including at least one of 6 SNP sites, can be applied to the molecular assisted breeding of Scophthalmus maximus, the screening can be carried out at the genome level through the breeding method disclosed in the present application, the breeding cycle can be greatly shortened, the dependence on phenotypic traits is reduced, the selection breeding efficiency is improved, and good application prospect is obtained.

The present application relates to the SNP molecular marker of the body weight correlation under the fermented soybean meal feeding of Scophthalmus maximus and application thereof, belongs to the molecular marker technical field, the present application utilizes the whole genome correlation analysis to carry out the analysis to the body weight character of Scophthalmus maximus, obtains the SNP molecular marker of the significant correlation under the fermented soybean meal feeding with the body weight character, including at least one of 6 SNP sites, can be applied to the molecular assisted breeding of Scophthalmus maximus, through the screening of the breeding method disclosed in the present application in the genomic level, can greatly shorten the breeding cycle, reduce the dependence on the phenotype character, improve the selection breeding efficiency, and have good application prospect.

The invention belongs to the technical field of fish breeding, and particularly relates to a fish seminal plasma exosome, an extraction method and application in fish breeding. The extraction method of the fish seminal plasma exosome comprises the following steps: S1, centrifuging fish seminal fluid to obtain seminal plasma; s2, mixing the seminal plasma with PBS and BPS according to a certain volume ratio, and standing; s3, centrifuging the standing liquid to obtain a precipitate I; s4, centrifuging the precipitate I to obtain exosome precipitate; s5, adding seminal plasma into the exosome precipitate in proportion, mixing and resuspending to obtain a mixed solution I; s6, centrifuging the mixed solution I obtained in the step S5 to obtain supernate rich in exosome particles; and S7, placing the supernatant in an EPF column for centrifugation. The fish seminal plasma exosome can be used for in-vitro fertilization and artificial insemination pretreatment of fish sperms, especially in-vitro fertilization and artificial insemination pretreatment of turbot sperms, and can improve the fertilization success rate, the sperm activation efficiency and the sperm motility.

The invention relates to an SNP molecular marker related to the body length of scophthalmus maximus fed with fermented soybean meal and application of the SNP molecular marker, and belongs to the technical field of molecular markers, the body length character of the scophthalmus maximus is analyzed through genome-wide association analysis, the SNP molecular marker remarkably related to the body length character of the scophthalmus maximus fed with the fermented soybean meal is obtained, and the SNP molecular marker comprises at least one of six SNP loci; the scophthalmus maximus molecular marker can be applied to scophthalmus maximus molecular assisted breeding, screening is conducted on the genome level through the breeding method, the breeding period can be greatly shortened, dependence on phenotypic characters is reduced, the selective breeding efficiency is improved, and the scophthalmus maximus molecular marker has good application prospects.