188 results about "Turbot" patented technology

The invention relates to a construction method of a fuzzy neural network expert system for the water quality assessment in turbot culture, which comprises neural network modeling and network model testing. The method is characterized in that the neural network modeling comprises the following steps of: setting network training parameters, wherein sensitive indexes of the growth of turbots after three-tiered classification, which include four expert data of temperature, salinity, pH and dissolved oxygen, are used as input parameters; determining the network topology: constructing a three-layer neural network by using the input layers of four input nodes, the hidden layers of two hidden nodes and the output layer of one output node; and determining training samples. The network model testing comprises the steps of: leading in tested samples and assessing the network model. The invention combines the neural network, the fuzzy system and the on-line monitoring system for the water quality assessment in industrial turbot culture for the first time, avoids the operations such as manual setting and the like in the traditional assessment method, and overcomes the influence of human factors and the like on the assessment in the traditional turbot water quality assessment.

A composition is for feeding aquacultural prey organisms such as Artemia and rotifiers, comprising a lipid component comprising at least 25 wt % of phospholipids and providing a DHA content of at least 30 wt %. The lipid component is preferably derived from marine organisms such as fishineal, phytoplankton or zoo plankton biomass. The composition is used for providing prey organisms having a high content of highly-unsaturated fatty acids (HUFAs) suitable for aquaculture of fish including halibut, turbot, bass and crustaceans and molluses.

The invention relates to a microsatellite multi-PCR (Polymerase Chain Reaction) for a turbot paternity test, which comprises the steps of: extracting DNA from an individual turbot, screening pleomorphic microsatellite markers of the turbot, synthesizing a primer, constructing turbot multi-PCR systems and carrying out genealogy and paternity tests. In the method, two multi-PCR systems are established for the turbot genealogy and paternity tests, the steps of multi-PCR amplification, electrophoresis and silver staining are carried out on an individual to be tested so as to acquire microsatellite marker gene type data, cluster analysis for a genealogy test is carried out by adopting NTsys sofeware, and the paternity test is carried out by adopting Cervus2.0 software. The invention realizes that four microsatellite sites are simultaneously detected in one PCR, thus compared with the traditional PCR method, the efficiency of the method is improved by around 4 times, and the method can be subjected to the field test in a breeding base. The invention has the characteristics of high efficiency, economy, simplicity, convenience, easy operation and the like and can be popularized and applied in the aspects of germplasm identification, genealogy management and selective breeding of fine breeds for the turbots.

A composite feed for the young turbot is prepared from 14 raw materials including anabarilius powder, spirulina powder, concentrated soybean protein, squid powder, beer yeast, soybean lecithin, etc. Its advantages are rich nutrients, high conversion rate, high growth speed, and less pollution to water.

The concentrated feed for adult turbot consists of: alpha-starch 20-25 wt%, Peru red fish meal 30-40 wt%, soybean dregs 10-20 wt%, wheat flour 5-12 wt%, squid bowel powder 5-10 wt%, animal bone meal 5-10 wt%, beer yeast 3-7 wt%, and other seven kinds of material. The feed of the present invention has the features of: capacity of providing adult turbot under artificially raising with all the nutrients, good taste, high feed conversion rate, high stability in water, functions of promoting the growth of adult turbot and raising it disease resistance and survival rate, low cost, etc.

The invention discloses a traditional Chinese medicine preparation for treating pathogenesis of turbot of aquatic animals. The traditional Chinese medicine preparation is prepared from the following ingredients in parts by weight: 30-40 parts of sweet wormwood, 15-25 parts of scutellaria baicalensis, 10-20 parts of areca catechu, 25-35 parts of szechwan Chinaberry fruit, 5 parts of fructus quisqualis, 4 parts of cortex meliae, 3 parts of pumpkin seed, 4 parts of common carpesium fruit, 4 parts of Chinese torreya, 3 parts of fructus ulmi, 3 parts of cyrtomium fortunei, 5 parts of dandelion and 3 parts of herba violae. Compared with the radix sophorae flavescentis powder of common medicine, the cost of the preparation is reduced by 50%; the dosage is reduced by 70%; the cure rate is improved by 25%; the preparation is high in bioavailability and low in additive amount; the preparation is fed with baits and convenient to use.

The invention relates to a turbot fin cell line forming method. It includes the following steps: using pectoral fin and pelvic fin tissue as raw material; shearing the fin tissue adopting trypsin, hyaluronidase, II type collagenase multiple step digestion process to gain free fin cell and loosen tissue block; and culturing in L-15 culture solution containing 20% fetal calf serum, carboxymethyl chitin, N- acetyl glucose hydrochloride, glucosamine hydrochloride, human epidermic cell growth factor, human alkali fibroblast growth factor, and tooth bothid gill cell culture solution; adopting trypsin digestion process to do sub-culturing. The technology is scientific. The formed sub-culturing cell reaches the 62th generations. The formed turbot fin cell is not transfected by any virus or oncogene, and can hopefully be directly applied to correlation theory study and viral vaccine development.

The invention discloses a turbot pathogenic strain which is Acinetobacter lwoffii, wherein the collection number of the strain is CCTCC (China center for type culture collection) NO:M2010372. The screened Acinetobacter lwoffii is used for preparing an inactivated vaccine. In addition, the Acinetobacter lwoffii, tetraodotoxin pseudoalteromonas and pseudomonas are used for preparing a bigeminy or trigeminy inactivated vaccine which is used for immunizing the turbot ascites disease. The preparation process of the inactivated vaccine for the ascites disease is simple, the yield is stable, the cost is low, and a large amount of inactivated vaccines with high immune effect can be obtained. The immune protective rate of the inactivated vaccine prepared by the method can reach 70%. The vaccine is a pure biological agent, has the characteristics of safety and environment friendliness, and dose not causes medicament residues and environment pollution; and by using the method, the generation and popularization of the turbot ascites disease are fundamentally suppressed, and a technical support is provided for preparing the novel inactivated vaccine for the turbot ascites disease.

The invention belongs to the technical field of marine organisms, and concretely relates to a method for the efficient ultralow temperature cryopreservation of turbot sperms. The method comprises the following steps: mixing the seminal fluid of turbots with an antifreeze liquid according to a volume ratio of 3:1-4:1, carrying out two-step cooling treatment, carrying out one-step balancing, adding to liquid nitrogen (having a temperature of -196DEG C), and preserving; and the antifreeze liquid is composed of a diluent, an antifreeze agent and an additive. High-quality frozen sperms obtained through the method for the efficient ultralow temperature cryopreservation of turbot sperms are of great significance of the artificial breeding, germplasm preservation, heredity diversity and sustainable culturing of the turbots.

The invention discloses a turbot cultivation net cage having an anti-pollution function, and relates to a turbot cultivation net cage. The turbot cultivation net cage uses a hot-dip galvanized iron pipe, a cylindrical plastic foam floating ball, a high-strength single fast knot polyethylene net, a high-strength nylon warp knitting net, an anti-pollution copper alloy rhombus net, a high-strength fishing polyethylene rope, an ultrahigh-strength rope and high-strength fishing lines and is characterized in that: a net cage floating support is welded into a top frame and a bottom frame by using the hot-dip galvanized iron pipe; the cylinder plastic foam floating ball under the lower surface of the bottom frame of the floating support is coated with waterproof cloth and a small-mesh waste netting; a netting system for the net cage consists of a double-layer synthetic fiber netting serving as a first netting and the anti-pollution copper alloy rhombus net serving as a second netting; the first netting and the second netting are connected in a vertical half-soft mode; the net bottom of the net cage is hung with a square galvanized iron pipe framework with edges of 5.0 meters; and the net cage floating support is connected with a floating rope frame through a cable and tied on a berth fixing system.

The invention relates to a detection method of a bastard halibut rhabdovirus in the aquatic livestock pathogen detection and diagnosis technical field. A real-time quantitative reverse transcription-polymerase chain reaction technology is adopted to detect the bastard halibut rhabdovirus of fish. A specific primer sequence and a fluorescence probe sequence are designed firstly and then nucleic acid of a sample to be detected is extracted; secondly, the real-time quantum reverse transcription-polymerase chain reaction is carried out, and ribonucleic acid containing target amplified fragments by in vitro transcription is used as a standard substance to draft a standard curve; finally, whether the sample to be detected contains the bastard halibut rhabdovirus is judged according to circulating valve value of the sample to be detected calculated by an apparatus software. The method of the invention can be widely applied to epidemic disease monitoring over aquatic plants of bastard halibut, weever, turbot, snapper sea bream, etc. and detection of the bastard halibut bullet-shaped virus in import and export trade. Compared with the prior art, the method of the invention is characterized by rapid detection, high efficiency, accurate quantum, strong specificity, wide detection scope, high sensitivity, etc.

A turbot parent fish feed belongs to the field of aquatic animal nutrition and feed. A basal feed comprises the following materials by mass percentage: 70% of fish meal, 4% of casein, 15% of high-gluten flour, 2% of phospholipids, 1% of choline, 0.5% of vitamin mixture, 0.5% of mineral mixture, 1.5% of dicalcium phosphate and 5.5% of fish oil; then 4800 mg/kg of vitamin C is added to the basal feed; and the added vitamin C is vitamin C poly-phosphate with 30% of effective content. The vitamin C of polyphosphate is added in the turbot parent fish feed to make the effective content of the vitamin C reach 4800mg/kg, so that the turbot spawning rate can be improved, the fecundity can be improved, the egg quality is improved, and the survival rate and the growth rate of the postlarva can be improved.

The invention discloses an artificial mixed fodder and its preparation method for turbot albation fry skin color improvement and restoration, wherein the ingredient comprises fish meal, yeasts, flour, soya bean waste flour, groundnut meal, shrimp husk, sea weed powder, KH2PO4, wheat bran, multivitamin, minerals, glucose, fish oil and phosphatidy lcholine.

The invention discloses a bacterial inhibition type water purifier for marine aquaculture and a preparation method thereof. The bacterial inhibition type water purifier is prepared from filtering and drying a fermentation solution containing bacillus subtilis YB-01 CGMCC-No.9756 and brevibacillus laterosporus YB-02 CGMCC-No.9757. The strain of the water purifier is bacillus separated from a seawater source and has an excellent water purification effect and bacterial inhibition effect; the water purifier is a mixture containing bacteria and spores; the water purifier is long in preservation period and can quickly grow after entering the water; especially, the water purifier is regularly sprayed in seedling periods and developing periods of stichopus japonicas, turbot and fugu rubripes; the harmful materials such as ammonia nitrogen, nitrite and hydrogen sulfide in the marine aquaculture water can be quickly degraded; the heavy metal ions in bottom mud can be efficiently adsorbed; deterioration, acidification and smelly bottom mud of the water can be avoided; the growth of pathogenic bacteria in the marine aquaculture can be inhibited; diseases can be effectively prevented.

The invention relates to artificial pellet feed for adult turbot. The artificial pellet feed is characterized by comprising white fish meal, red fish meal, whey protein, soybean protein concentrate, soybean meal hydrolysate small peptide, scallop powder, chicken meal, high protein flour, alpha-starch, L-alanyl-L-glutamine, choline chloride, sea fish oil, soybean lecithin, krill meal, ocean red yeast, photosynthetic bacteria, Schizochytrium limacinum powder, haematococcus pluvialis powder, multivitamins, composite mineral substances, Chinese herbal medicines and sodium alginate. According to the feed, a plurality of kinds of raw materials are added into the feed, so that the comprehensiveness of nutrition is guaranteed. During actual culture of the turbot, the pellet feed for the adult turbot has the advantages that the feed is good in feeding promoting performance, fishes like the feed, are full easily and do not fall ill easily, the survival rate is high, and the feed coefficient is low; the digestibility and the feed conversion ratio are high; the growth rate of the fishes is high, and the culture cycle is short.

The invention relates to feeding attractant used for turbot. The feeding attractant is characterized by consisting of trimethylamine hydrochloride, betaine, dimethyl-Beta-propiothetin, citric acid, taurine, sodium glutamate and aminoacetic acid; the weight percent (w/w) ranges of the components are as follow: 25 to 35, 25 to 35, 10 to 20, 5 to 10, 5 to 10 and 5 to 10. The feeding attractant used for turbot is used as additive in turbot feed, and only 0.5 percent to 1.5 percent of the feeding attractant is added. The feeding attractant used for turbot has the advantages that: (1) the feeding attractant is used in the turbot feed, and only a small amount of the feeding attractant is added; (2) the feeding attractant can obviously promote the ingestion and the growth of the turbot; meanwhile, can reduce residual bait, and is good for environment protection; (3) the feeding attractant can greatly reduced the consumption of fish meal in the feed, and reduce the feed cost; (4) the raw materials of the invention have stable sources, low cost and simple production process.

The invention relates to the technology of chromosome operation, in particular to a method for inducing turbot homogenesis gynogenesis fry by using the theory that heredity inactivated sperms are used to perform artificial insemination and a fertilized ovum is restrained from cleavage through hydrostatic pressure. Sexually mature turbot parent fishes are selected, seminal fluid and spawn are collected respectively, insemination is performed on the seminal fluid and the spawn in a volume ratio of 1:100-200, and a control group is formed. The surplus seminal fluid is diluted by a precooled Ringer solution, and sperms are inactivated by ultraviolet rays. Insemination is performed on the inactivated seminal fluid and ova in a volume ratio of 1:10-20. In the 10-20 min before the first fertilized ovum crack appears, the fertilized ovum is placed in a hydrostatic press pressurizing vessel, and processing is performed by the hydrostatic pressure of 55-75 MPa for 6-8 min. Through the method, the 100% turbot homogenesis gynogenesis fry can be inducing obtained efficiently, stably and reliably.

The invention discloses a detection method of a turbot FF0901 microsatellite marker by utilizing specific primers, comprising the following steps: firstly extracting turbot genomic DNA; then utilizing a sequence containing a microsatellite in an enrichment library of the turbot microsatellite; designing specific primers at two ends of the sequence as a positive strand 5'-TTACGCTTCGCATCGCTCAT-3', and a negative strand 5'-TGCCAGGCCACCAGACG-3'; carrying out PCR amplification on the turbot genomic DNA; separating the obtained PCR product through modified polyacrylamide gel electrophoresis; analyzing strips generated on the product to obtain a turbot genetic polymorphism map. The method of the invention is suitable for the detection technologies of turbot population genetic marker, genealogy authentication, genetic linkage map construction and the like; the PCR amplification product presents better polymorphism in turbot population detection; and the method of the invention can rapidly acquire a genetic variation map of the turbot FF0901 microsatellite marker, and conveniently, rapidly, accurately and intuitively detect each individual genotype of the turbot.

The invention relates to an artificial microparticle feed of turbot postlarva, and the artificial microparticle feed is characterized by including the following feed components: white fish meal, krill meal, squid meal, brewer yeast, starch, cod liver oil, egg yolk powder, whey powder, L-alanyl-L-glutamine, choline chloride and soy lecithin. The artificial microparticle feed may also include the following feed components: schizochytrium aggregatum powder, a compound vitamin, a compound mineral, gelatin and sodium alginate. A plurality of raw materials are added into the artificial microparticle feed to ensure comprehensive nutrition, immunopotentiators are added into the artificial microparticle feed to enhance fish-body non-specific immunity, the artificial microparticle feed is pollution-free and green, and the schizochytrium aggregatum powder is added into the artificial microparticle feed for effective reduction of the fry albino rate.

The invention aims at providing an evaluation method of individual breeding values of turbot to solve the problems that a using background is not clear in source, a physical pedigree is incomplete or the individual kinship coefficient can not be calculated by utilizing physical pedigree information when an introduced turbot colony is used for breeding improved varieties, further solve the problem that evaluation of individual genetic parameters can not be performed and thus make up for deficiencies in the prior art. Compared with a method for evaluating the breeding value by relying on physical pedigree, the method provided by the invention can greatly reduce the time period for evaluation of the individual breeding values, namely that the evaluation of the individual breeding values can be completed by only one generation without performing multi-generation of culture of pedigree materials and other tedious steps.

The invention relates to a method for preparing smoked turbot fillets, which comprises the following steps of: cultivating food raw materials temporarily, sacrificing, peeling skin off, slicing, pickling, drying, smoking, drying again, finishing, freezing quickly, probing by using gold and boxing, wherein the temperature of the food which is dried twice and smoked is below 20 DEG C; performing special seasoning by using pickling solution of table salt, granulated sugar and water; and selecting a high-quality hardwood jujube sawdust smoking material with low resin content, and smoking at the cold smoking temperature of below 20 DEG C according to the conventional process for smoking fish. By the method, the primary taste essence of turbot can be kept, damage to nutritional ingredients is minimum, and the method is safe and sanitary; and characteristic smoked fish products which are delicious in taste, tender in meat quality and golden yellow in color and luster can be produced, wherein the content of benzopyrene (a) in the products is 0.1ug/kg and is far below the limit standard, which is specified by GB7104-94, of smoked fish, namely is less than or equal to 5ug/kg, and the smoked turbot fillets do not contain clostridium botulinum by detection.

An efficient cultivation method of a turbot family offspring comprises the following steps: parent fish selection, parent fish cultivation, artificial directional mating, hatching, offspring cultivation and getting of commercial offsprings. The parent fish is selected from germplasm parent fish of different batches from different countries; artificial fertilization is performed among different germplasm parent fish, and the same batch of the parent fish are not allowed to mate with each other; offspring cultivation is as follows: at the early period, the adopted facility is a 3m glass reinforced plastics barrel, and bait comprises chlorella, rotifer and artemia; at the middle period, the adopted facility is a 10m cement pond, and the bait is micro pellet bait; at the later period, the adopted facility is a 20-30m cement pond, and the bait is fish larvae pellet bait; and specification of the commercial offsprings is 5-6cm. The method is characterized by adopting the parent fish with different germplasm resources for the artificial directional mating, and seriously controlling inbreeding; and adopting different culturing facilities to cultivate the offspring by three stages, which causes the offspring emergence rate of the commercial offspring to reach 46.8%, and improves growth rate thereof by 36%, and lowers of etiolation rate thereof below 5%. The method can efficiently produce high-quality turbot offsprings, and is applicable to large-scale production.

The invention provides a method for improving the success rate of establishing father half- sib family through the selective breeding of turbot family and comprises the steps that first, parental turbots used for family selective breeding are cultured as parental fish and marked individually; second, the sex gland of the parental turbot can achieve synchronous maturity by reproductive control; third, the parental fish, the sex gland development of which is in the peak stage, is selected for artificial insemination; then the father half-sib family is established according to the method that one male fish is mated with 2-3 pieces of female fish. The establishment of the father half-sib family is carried out again if the initial establishment fails. The invention applied to the selective breeding of the turbot family has simple operation and remarkable effect and the success rate of the initial establishment of the father half-sib family can be remarkably improved to more than 33.3 percent; the established father half-sib families have good synchronism, which dramatically shortens the family establishment period and is beneficial for the standard culture of the turbot family.

The invention relates to a feed for fish, and in particular relates to a high-quality compound feed for adult turbots and a preparation method thereof. The compound feed is prepared from the following components in parts by weight: 20-30 parts of fish meal, 10-20 parts of bean pulp, 8-14 parts of clam worm powder, 8-14 parts of enteromorpha powder, 8-12 parts of bran, 6-12 parts of rice bran, 6-10 parts of sunflower seed kernel meal, 6-10 parts of cottonseed meal, 4-8 parts of corn flour, 4-8 parts of coix seed, 4-6 parts of mung bean flour, 4-6 parts of dried potato powder, 4-6 parts of cordate houttuynia, 3-5 parts of purslane, 3-5 parts of capsella bursa-pastoris, 1-3 parts of dried hawthorn powder, 2-4 parts of fish oil, and 2-4 parts of honey. The compound feed has the advantages that the feed coefficient is low, and the turbots fed by the feed are delicious in meat and high in muscle quality.

The invention discloses a low-fishmeal jewfish extruded compound feed for resource utilization of salicornia bigelovii. The disadvantage that existing feed fish meal is high in dosage is overcome, wastes are changed into treasures, and salicornia bigelovii resource is developed and utilized at high value. The feed product is mainly prepared from raw materials of salicornia bigelovii ferment powder, steam fish meal, corn starch, bean pulp, sesame seed meal, rapeseed dregs, molasses yeast, fish oil, animal fat, sodium alginate, soy bean lecithin, monocalcium phosphate, decavitamin, composite mineral substance, allicin, bacillus subtilis, lactobacillus, amylase and non-starch polysaccharide enzyme; the salicornia bigelovii ferment powder is prepared from the raw materials of astragalus membranaceus, imperata cylindrical, liquorice, schisandra chinensis, hawthorn powder and medicated leaven through a two-step fermentation technology. The product has the advantages that the fast growth of turbot is significantly promoted, the breeding survival rate is increased, the breeding cost is reduced, the breeding digestion and absorption rate is improved, nitrogen and phosphorus excretion is reduced, the breeding environment is improved, and the low-fishmeal jewfish extruded compound feed has relatively high cost performance and popularization and application value.