190 results about "Ciliate" patented technology

The invention provides a phytoremediation method for managing composite cadmium-lead-arsenic contaminated soil. The method comprises the following steps: step S1: selecting a cadmium-enriched castor-oil plant variety; step S2: obtaining cadmium-lead-arsenic composite contaminated soil with neutral acidity and alkalinity; step S3: planting cadmium-enriched castor-oil plants and ciliate desert-grass in an intercropping manner in the cadmium-lead-arsenic composite contaminated soil with neutral acidity and alkalinity for surviving and growth, and harvesting matured castor-oil plants at regular intervals, and carrying out step S5; step S4: harvesting the upper part of the ciliate desert-grass according to the growth height of the ciliate desert-grass, and carrying out the step S5; retaining stubble of the ciliate desert-grass on the soil surface, and carrying out the step S3; step S5: feeding the harvested and matured castor-oil plants and ciliate desert-grass to an exclusive place for concentration and carrying out innocent treatment. The invention further provides a phytoremediation method for managing cadmium contaminated soil. The phytoremediation method is suitable for managing and repairing cadmium-lead-arsenic moderately and slightly contaminated soil, and has good practical application prospect.

The present invention is related to a system for the heterologous expression of a monoclonal Antibody (mAb) or a fragment or derivative thereof, said system comprising at least one ciliate host cell, and incorporated, into said ciliate host cell, at least one heterologous nucleic acid molecule encoding for said monoclonal Antibody, or a fragment or derivative thereof.

The invention provides a microorganism bactericide capable of reinforcing plants to restore fields polluted by arsenic and preparation and application methods thereof, belonging to the technical field of environment restoration engineering. The strain utilized by the bactericide is Agrobacteriumsp. C13. The bactericide adopts a composite carrier which comprises turfy soil, saw dust, rice hulls and active carbon with the mass percentage being 30:25-30:25-30:15; the microorganism fermentation liquor is mixed with the composite carrier according to the mass ratio of 0.5-1:1; and the adsorption amount of the microorganisms of the composite carrier reaches 109-1012 per gram. The prepared bactericide is applied to the arsenic-polluted fields restored by ciliate desert-grass according to the use amount of 5g/kg, the biomass liveweight of the overground part of the restored plants is improved by 16-17%, and the removal rate of arsenic in the fields is improved by 40-41% in comparison. The carrier of the bactericide in the invention has rich raw materials, simple preparation method and easy realization of large-scale production.

The invention discloses a multi-nutrient block for promoting fattening of cattle and sheep and breeding stock health. The brick comprises the following components: salt, mixed vitamins for cattle and sheep, gypsum, calcium hydrophosphate, anhydrous magnesium sulfate, honey, organic copper, organic iron, zinc methionine, organic zinc, anhydrous manganous sulfate, calcium iodate, sodium selenite, cobalt chloride, a rumen microbial nutrition agent, bentonite and a food pigment. The components adopted by the brick are easily absorbed by the cattle and sheep, and meanwhile, the rumen microbial nutrition agent is added, so that the environment of microbial synthetic protein in rumen can be better ensured, and meanwhile, required nutrients for bacteria and ciliates in the rumen are also provided. By adopting the brick, the digestibility of energy and protein can be greatly improved, the digestibility of the rumen is improved, the biological value of a feed is improved, and the multi-nutrient block is abundant in nutrient, and applicable to a plurality of stages of cattle and sheep with different growth conditions and different physiological regions.

Disclosed is a Chinese herbal composite prescription for controlling sea fish paralembus digitiformis, wherein the medicinal constituents include (by weight ratio) European verbena 40%, Kulian 30%, baikal skullcap root 30%, the preparing process consists of disintegrating, passing through 100 mesh sieve, and mixing homogeneously.

The invention discloses a method for processing wastewater containing arsenic by using the ciliate desert grass artificial wetland. Super concentration plant ciliate desert grass which is planted in a drowned flow wetland system is utilized to process wastewater containing arsenic, cobblestones are used as the loading material of the drowned flow wetland system, soil with a certain thickness is covered on the groundmass of the drowned flow wetland system, and the arsenic in water body is continuously extracted till the content of arsenic in the water body reach the corresponding state environmental standard. With the advantages of high efficiency, low investment, low running, low maintenance technology, easy popularization and application, etc., the method has no secondary pollution, and meanwhile the ecologic system can not be damaged during recondition process, the method also can facilitate the soil or the water degradation and the falling of the productivity caused by the arsenic pollution of the soil and the water, and recover and improve the diversity of the soil or the water life-forms.

The invention relates to a method for preparing and culturing a culture medium for the supravital culture of seawater scuticociliatida ciliates, and relates to the field of the study of aquatic animal diseases. The method for preparing the in-vitro culture medium comprises the following steps of: mixing fresh brain tissue and muscular tissue of paralichthys olivaceus or turbot fish in a mass ratio of (1:1)-(3:1), and homogenizing to form muddy flesh; adding sterilized seawater into the muddy flesh in a mass/volume ratio of the muddy flesh to the seawater of (1:45)-(1:55), and performing waterbath at the temperature of between 65 and 70 DEG C for 2 hours to prepare the in-vitro culture medium, wherein the prepared culture medium is stored at the temperature of 4 DEG C for a long time. Themethod for preparing the culture medium is simple, and complex components are not needed to be added; and the seawater scuticociliatida ciliates are subjected to the supravital culture by utilizing the culture medium prepared by the method, and the scuticociliatida ciliates can survive for over 30 days, so that the foundation is established for the further study of the pathogenesis and preventionand control technology of the scuticociliatida ciliates.

The invention discloses a method for culturing the green spheroids of a pteris vittata and a special medium thereof. In the method, the rhizomes of ferns are used as explants and are cultured by a plant tissue medium to obtain the green spheroids; the plant tissue medium is a medium obtained by adding cane sugar with a final concentration of 18 to 22g/L, agar with a final concentration of 4 to 6g/L, GA with a final concentration of 0.3 to 0.7g/L and 6-BA with a final concentration of 0.4 to 0.6g/L into a 1/2MS-MS medium; wherein, the final concentration refers to the concentration in the plant tissue medium. Experiments show that the induction rate of the green spheroids obtained by the medium and the culturing method can achieve more than 70 percent. The differentiation rate of the green spheroids to carry out bud initiation can achieve 98 percent. Moreover, the method has the characteristics of short time, low cost and simple operation.

The invention relates to a plant restoration method of tetracycline-polluted wastewater, which comprises the following steps: selecting flat foam plastic as a floating bed, making holes which are uniformly distributed in the floating bed, and growing 1-2 ciliate desert-grass plants in each hole, wherein the distance between every two adjacent holes is 10-15 cm; discharging tetracycline-polluted wastewater to be treated into a water storage tank; putting the floating bed in the water storage tank, wherein the root of the ciliate desert-grass is completely immersed in water, and the overground part of the ciliate desert-grass is above the water; and finishing water body restoration within 4-6 days. The invention does not have special requirements for the pH value of the polluted water body, has the advantages of high tetracycline removal efficiency, low investment, low maintenance cost, small engineering work load, low energy consumption and the like, is simple to operate, can be used repeatedly, does not generate secondary pollution, and is a green restoration technique with wide application range and favorable effect.

The invention relates to pesticide for controlling corythucha ciliate. The pesticide is characterized by comprising the following materials according to the volume ratio: 2 parts of 3% acetamiprid cream, 1-5 parts of 5% imidacloprid cream and 0.5-5 parts of 30% acephate. The invention also provides a use method of the pesticide. The pesticide has the advantages that the relative conduction velocity of the acephate is faster, marked pests are quickly killed, so that the pesticide is used as a main pesticide agent; after the pesticide is applied for 7 days, the content of the pesticide reaches to be highest; the pesticide effect of imidacloprid and acetamiprid at the early stage of application is not obvious, so that the imidacloprid and the acetamiprid are used as auxiliary pesticides matched with the main agent for use; the controlling effect on the corythucha ciliate is good, the lasting time is longer, and the preventing effect is more than 95% after the pesticide is applied for 40 days; and the pesticide has better lasting effect by dry-injection use.

The invention discloses a plant restoration method for a tailings ponds. The plant restoration method includes: 1), planting cattail, commelina and ciliate desert-grass to the tailings ponds by adopting an interplanting manner; 2), sowing moss in areas without clear water within the planting area; 3), planting duckweed in planting area with clear water; 4), harvesting cattail, commelina and ciliate desert-grass with harmless treatment. With the method, the content of heavy metal in the tailings ponds can be effectively reduced, the service life of the tailings ponds is prolonged, and the vegetation coverage of retired tailings ponds can be improved.

The invention discloses a culture medium for tissue culture of toona ciliate roem. The culture medium comprises a subculture medium and a rooting culture medium. A tissue culture manner is used for carrying out germchit vegetative propagation for the first time; compared with the prior art, the culture medium has the advantages that the seed propagation is rapid so that the good property of female parents can be effectively kept and the quality of germchits is guaranteed.

The invention provides a preparation method and application of biochar with ciliate desert-grass enriched with a copper element. The method comprises the steps that 1, screening and transplanting robust seedlings, irrigating the seedlings with a Cu<2+> metal solution once every 3-5 days to enrich copper for 1 month, harvesting the ciliate desert-grass to obtain the ciliate desert-grass rich in copper, wherein blank control is water irrigated by water; 2) digging up the copper-enriched ciliate desert-grass and blank control plants, washing away soil at the roots and cutting the roots of the plants into pieces, drying the pieces in a drying oven respectively, and storing the pieces for later use; 3) respectively taking the dried blank control ciliate desert-grass root system and the copper-enriched ciliate desert-grass root system in a crucible, exhausting air under a sealed condition, feeding nitrogen until the pressures inside and outside the crucible are balanced, and adjusting the nitrogen flow rate to 15-25 ml/min and sintering the materials at 500-900 DEG C to obtain biochar; 4) grinding the biochar, pouring the biochar into a 3-6 mol/L NaOH solution, stirring the liquid for 1-3 hours at 70-85 DEG C, filtering and water-washing the material, standing the material, removing floating dregs, and drying the material to obtain the biochar with ciliate desert-grass enriched witha copper element, which has a good catalytic degradation effect on methylene blue under the catalysis of visible light.

A tissue culture method of the spores of ciliate desert-grass for fast reproducing it in order to repair the As-polluted soil includes such steps as taking the mature spores of the ciliate desert-grass, culturing them in the culture medium prepared from MS, KT and NAA for inducing germination, secondary culturing in the culture medium prepared from MS, KT, NAA, agar and cane sugar, or from Knop and NAA for inducing rooting, pratising seedlings, and culturing the seedlings.

The invention provides four-season health-care tea which comprises the following components in part by weight: 1-5 parts of black tea, 0.1-1 part of lucid ganoderma, 2-8 parts of polygonum multiflorum, 0.2-0.9 part of sophora flower, 1-8 parts of lotus seed, 0.3-0.9 part of black sesame, 2-10 parts of raisin, 0.2-0.9 part of rose, 0.1-0.8 part of honeysuckle, 0.1-0.8 part of rhizoma alismatis, 0.5-5 parts of sweet-scented osmanthus, 0.2-0.9 part of hypochaeris ciliate, 0.1-0.8 part of dark plum, 0.1-0.7 part of longan pulp and 0.2-0.8 part of red date, and a preparation method of the four-season health-care tea. Through reasonable preparation, the four-season health-care tea sufficiently brings the synergistic effect of the components into play, has the functions of clearing the heat, expelling the toxin, beautifying and enhancing the resistance, and is simple in preparation process, low in cost and easy to produce.

The invention discloses a cultivation method for intercropping ciliate desert-grass and corn through recycling. By utilizing the characteristics of low accumulation of heavy metal arsenic in corn grains and high enrichment of heavy metal arsenic by perennial plants ciliate desert-grass, intercropping is conducted in cultivated land heavily polluted by heavy metal arsenic; the method comprises thefollowing specific steps of deep scarification of soil, ditching for application of corn base fertilizer, plant-shrinkage, closely-planting and optimized sowing of corn, intensive cultivation in a corn vegetative period, seedling-staggered method transplanting of the ciliate desert-grass, precise management of a ciliate desert-grass field, stubble-remaining cutting of the ciliate desert-grass andcoverage of corn straw, and circular planting of the corn and sustainable utilization of the ciliate desert-grass. Compared with a traditional habitual planting mode, the method has the advantages that through the specific steps, the yield of the corn grains can be significantly increased, the green-turning survival rate and biological yield of the ciliate desert-grass are significantly increased,and the absorption and treatment effect on heavy metal arsenic in the soil is significantly improved.

The invention discloses a traditional Chinese medicine bath agent for treating pruritus cutanea after hematodialysis. The pruritus cutanea belongs to the domain of 'universal eczema' of the traditional Chinese medicine. The traditional Chinese medicine believes that skin malnutrition is mainly caused by deficient nutrient-blood, non-nourishing of the liver by blood, endogenous wind and blood depletion, resulting in itching, and 'deficiency, humidness, stagnation and wind' are taken as basic pathogeneses. The method for nourishing the blood, promoting blood circulation and eliminating dampness is commonly adopted in treatment of the traditional Chinese medicine. The traditional Chinese medicines such as pair vetch root or leaf, needle filings, autumn quince, snakeroot, Japanese conehead herb, pine needle, acanthopanax ricinifliux seem root, ombre manila flowers and ciliate leucas root are selected to be decocted, so as to prepare the bath agent. A clinic test proves that the total effective rate can be up to 88.9% and much better than that of a control group, and the curative effect is worthy of being affirmed.

The invention relates to a method for remediating heavy metal contaminated soil through combination of carex nubigena and ciliate desert-grass, and belongs to the technical field of environment protection plant remediation. The method for remediating heavy metal contaminated soil through combination of the carex nubigena and the ciliate desert-grass includes the four treatment steps of seedling culture, interplant, harvesting and incineration. Firstly, seedling culture of the ciliate desert-grass and collection of the carex nubigena are completed; then ciliate desert-grass seedlings growing to 40 cm are transplanted, the carex nubigena is transplanted between line spaces of the ciliate desert-grass, the carex nubigena and the ciliate desert-grass are cut 1-2 times every year, the carex nubigena and the ciliate desert-grass are reserved 3-8 cm on the ground during cutting for overwintering, and finally harvested carex nubigena and harvested ciliate desert-grass are both subjected to incineration in a metallurgical furnace for harmless treatment. The carex nubigena and the ciliate desert-grass are used for conducting combined remediation on the heavy metal contaminated soil due to the hyper-accumulation characteristic on lead, zinc, copper, cadmium and arsenic in the soil, the remediation effect is more obvious than that of a traditional soil remediation method, the method is clean and efficient, and the remediation cost is low.

The invention discloses a feed additive capable of improving anti-stress and immunity of ruminants and a preparation method thereof. The feed additive capable of improving the anti-stress capability and immunity of ruminants is prepared from the following raw materials: bacillus subtilis powder, lactobacillus plantarum powder, saccharomycete powder, catechuic acid, xylooligosaccharide, calcium fatty acids, chromium nicotinate, medical stone, vitamin C, vitamin B1, nicotinic acid, pine pollen, tricalcium phosphate, manganese sulfate, soy isoflavone, berberine, soya bean meal, versioolor swallowwort root, herba ephedra, futokadsura stem, herba potentillae chinensis, ford manglietia fruit, ciliate desert-grass, ajuga decumbens, tangerine leaves, cow soapwortseed and table salt. The feed additive capable of improving the anti-stress capability and immunity of ruminants contains multiplex vitamins, trace elements and Chinese medicinal herb ingredients, and all the ingredients achieve a synergistic effect, so that the intestinal micro-ecological environments of ruminants can be effectively improved, the quantity of pathogens is reduced, stress reaction is reduced to a great extent, the immunity is improved and thus the disease incidence of ruminants.

The invention discloses application of curcumin to preparation of drug for killing fish epizoic infusorians, particularly relates to application to preparation of drug for killing ichthyophthirius multifilis, and belongs to the technical field of drug for killing parasites. According to the application, the curcumin is applied to the preparation of drug for fish epizoic infusorians for the first time, particularly for killing the ichthyophthirius multifilis; the parasites can be completely killed in the effective dosage range; and the effect of preventing and controlling ichthyophthiriasis is achieved. The application has the advantages that the curcumin sources are wide; the cost is low; the raw materials are rich; the preparation process is industrialized; no residue or no pollution on environment exists; no poison or no harm is caused on the human body; the half lethal concentration (56.8mg/L) on fishes is 187.4 times of the medium effective concentration (0.303mg/L) for killing the ichthyophthirius multifilis; safety is achieved on the fish; huge potential for developing novel fish drug for preventing and treating the fish epizoic infusorians is realized; and the the application prospects are wide.