110 results about "Synthetic protein" patented technology

The present invention relates to methods and compositions for modifying peptides, polypeptides and proteins with polymers, especially glyco-mimetic polymers, so as to improve their biological activity or pharmacokinetic properties. The invention further provides methods and uses for such polymer-modified peptides, polypeptides and proteins. The invention is particularly suitable for use in the synthesis of polymer-modified synthetic bioactive proteins (FIG. 1D), and of pharmaceutical compositions that contain such proteins.

A method and a device are disclosed for monitoring the synthesis of proteins by the ribosome in real time, in vivo as well as in in-vitro. The ribosome is engineered to carry a donor fluorophore, and tRNA and/or amino acids and/or some other part of the ribosome are either engineered to carry acceptor fluorophores or else their natural fluorescent properties are utilized as acceptors. As the ribosomes mechanism processed the mRNA and tRNA molecules and synthesizes a polypeptide chain, a light source illuminates the ribosome, exciting the donor fluorophores and thereby the acceptor fluorophores whenever these are in sufficient proximity to a donor. The resulting signals are detected and used as a key for real-time database searching and identification of the protein being synthesized. The resulting data can be tabulated and interpreted in different ways. FIG. (1) describes the properties of a FRET pair and the dependence of FRET on pair distance.

The invention provides a preparation method of a yeast cell extracting solution, the yeast cell extracting solution and a method for synthesizing protein in an in vitro system by virtue of the yeast cell extracting solution, as well as a kit containing the yeast cell extracting solution. The kit, which is more convenient than a conventional method, is applicable to protein synthesis in the in vitro system; transformation, culture and crushing can be prevented; a great amount of using time and cost can be saved; various types of proteins can be expressed, and influence of protein toxicity can be prevented; and a plurality of protein complexes can be expressed, without the use of a high temperature at 37 DEG C. In addition, as a raw material adopted by the invention, yeast cell is simple to cultivate, convenient to operate, rapid to propagate and relatively low in cost; a prepared yeast extract has a capacity of modifying translated protein, the yeast extract is suitable for large-scale preparation and has an advantage of industrial production; and the yeast cell extract, which is prepared into freeze-dried powder by virtue of a vacuum freeze-drying method and is preserved at low temperature or high temperature, is convenient to transport.

The invention provides a protein synthesis system for in-vitro protein synthesis, a kit and a preparation method for a protein through in-vitro synthesis. Specifically, the in-vitro cell-free expression system provided by the invention is capable of extremely efficiently synthesizing proteins and synthesizing complex proteins. Moreover, due to the in-vitro cell-free expression system disclosed bythe invention, the relative light unit value of the activity of the synthesized luciferase is higher than that of the conventional commercial system (such as a rabbit reticulocyte in-vitro expressionsystem) by at least one order of magnitude (more than or equal to 10 times or higher).

In an embodiment, a number of synthetic protein triblock copolymers are provided comprising first and second end hydrophobic blocks separated by a central hydrophilic block. In particular, the synthetic proteins are elastin-mimetic proteins having improved mechanical characteristics and related methods of making the proteins with the capability of providing precise control over the mechanical properties. Provided are proteins used in a number of medical devices such as artificial blood vessels, shunts, stents or as embolic agents in situations where it is desired to stop or reduce blood flow or pressure in a localized region.

The invention discloses a multi-nutrient block for promoting fattening of cattle and sheep and breeding stock health. The brick comprises the following components: salt, mixed vitamins for cattle and sheep, gypsum, calcium hydrophosphate, anhydrous magnesium sulfate, honey, organic copper, organic iron, zinc methionine, organic zinc, anhydrous manganous sulfate, calcium iodate, sodium selenite, cobalt chloride, a rumen microbial nutrition agent, bentonite and a food pigment. The components adopted by the brick are easily absorbed by the cattle and sheep, and meanwhile, the rumen microbial nutrition agent is added, so that the environment of microbial synthetic protein in rumen can be better ensured, and meanwhile, required nutrients for bacteria and ciliates in the rumen are also provided. By adopting the brick, the digestibility of energy and protein can be greatly improved, the digestibility of the rumen is improved, the biological value of a feed is improved, and the multi-nutrient block is abundant in nutrient, and applicable to a plurality of stages of cattle and sheep with different growth conditions and different physiological regions.

A disclosed special feed for E.malabaricus is prepared by the following raw materials by weight ratio: 40-50 parts of fish meal, 10-15 parts of soybean meal, 6-10 parts of small peptide, 4-8 parts of shrimp meal, 4-8 parts of yeast powder, 12-16 parts of strong flour, 2-4 parts of fish oil, 1-2 parts of soya-bean oil, 1 part of choline chloride with a mass percent of 50%, 1 part of decavitamin, and 4 parts of composite minerals. After the special feed is added with small peptide, the yield of E.malabaricus in a cubic water body is improved by about 15 percent, the bait coefficient is reduced to less than 1.5, and the utilization rate of baits is substantially improved. Research shows that small peptide can be directly absorbed by animals and is taken as a substrate for synthesis of protein in cells, and plays an important role in digestion, absorption and metabolism of amino acid; also small peptide is capable of improving utilization rate of trace elements in the feed, promoting growth of fish bodies and enhancing immunity of the fish bodies. The special feed for E.malabaricus not only helps to enrich and develop theories of aquatic animal nutrition and feed science, but also helps culturists to acquire substantial economical benefit and social benefit.

The invention provides a GMP compatible method to chemically synthesize proteins which may be advantageously used in compositions for vaccination that are free of biological contaminants. The method uses conventional synthesis of peptides and linking these to yield synthetic proteins that preferably comprise all T cell epitopes for an antigen. Preferably an adjuvant is covalently attached to a synthetic protein to yield a fully synthetic vaccine. The invention is illustrated mainly by using HPV protein directed immunity as a model.

Compositions and methods effective in inhibiting abnormal or undesirable cell proliferation, particularly endothelial cell proliferation and angiogenesis related to neovascularization and tumor growth are provided. The compositions comprise a naturally occurring or synthetic protein, peptide, or protein fragment containing all or an active portion of the C-terminal portion of proteinase inhibitors such as TFPI. The methods involve administering to a human or animal the composition described herein in a dosage sufficient to inhibit cell proliferation, particularly endothelial cell proliferation. The methods are useful for treating diseases and processes mediated by undesired and uncontrolled cell proliferation, such as cancer, particularly by inhibiting angiogenesis. Administration of the composition to a human or animal having prevascularized, metastasized tumors is useful for preventing the growth or expansion of such tumors.

A cold fusion concrete formulation including a mixture of water, silicon based mineral aggregates acting as a filler material; sodium or potassium metasilicate/pentahydrate acting as an activator; waste from steel production including Granulated Ground Blast Slag acting as a cementitious ingredient; high calcium or low calcium waste from coal combustion (fly ash or bottom ash) acting as a cementitious ingredient; sodium tetraborate, sodium citrate dihydrate, citric acid, or boric acid acting as set-time retarders; strengthening agents including including calcium, potassium, magnesium, sodium, or aluminium hydroxides; attapulgite, kaolin, red, or other fine grained, high alumino silicate containing clay, for increasing the silicon and alumino-silicate concentration and associated strength; a protein or synthetic protein material to form a weak covalent bond with the hydroxides and silicates, for the purpose of maintaining a consistent volume during the curing process; and a pollinated fern oil to reduce water content of the mixture and decrease viscosity.

The invention belongs to the technical field of immune protein preparation and applications thereof, and particularly relates to a test paper for detecting African swine fever virus antibody. The invention provides a gene sequence E183L-1 for encoding an extracellular region of an African swine fever virus p54 protein. Based on a general inventive concept, the invention also proposes a primer pairfor amplifying the gene sequence and a synthetic protein encoded by the gene sequence. In order to solve the problems existing in the detection of the African swine fever virus, the invention prepares the test paper for detecting the antibody of the African swine fever virus by utilizing the synthetic protein encoded by the gene. The test paper can rapidly and accurately detect the antibody of the African swine fever virus, and is very suitable for basic-level and on-site rapid detection and diagnosis.

The invention discloses a pyrroloquiniline quinone (PQQ) synthetic gene cluster and a coded protein system thereof. The protein system consists of the following proteins: 1) proteins shown in SEQ ID No.3, SEQ ID No.5, SEQ ID No.7, SEQ ID No.9 and SEQ ID No.11; or 2) proteins which are derived from the proteins 1) with the amino acid sequences shown in SEQ ID No.3, SEQ ID No.5, SEQ ID No.7, SEQ ID No.9 and SEQ ID No.11 through replacing, deleting or adding one or more of amino acid residues, and have the same activities. The invention also provides the gene cluster for coding the protein system, wherein the sequence of the gene cluster is shown in SEQ ID No.1. The PQQ synthetic gene cluster and coded protein system provided by the invention play important roles in the PQQ biosynthesis, the improvement and construction of PQQ producing strains, the increase of small bacterium saccharic acid conversion efficiency and the construction of alcohol saccharic acid one-step conversion engineering bacteria.

The invention relates to a detection technology for detecting paralytic shellfish poisoning, in particular to a method for preparing an anti-paralytic shellfish poisoning monoclonal antibody by using protein coupling to synthesize an antigen, and relates to the use of monoclonal antibody and gonylin GTX2/3 antigen Combination, and the preparation method of the quick detection kit that analyzes the content of gonylin GTX2/3 in the biological sample. An enzyme-linked immunoassay kit for rapid detection of the main component of paralytic shellfish poisoning, gonyellin GTX2/3, including a 96-well microtiter plate with detachable strips, enzyme-labeled secondary antibody, and buffer salt wash containing Tween-20 solution, substrate solution and stop solution, using the prepared monoclonal antibody containing specific anti-gonitoxin GTX2/3, on the 96-well ELISA plate coated with the coated antigen of the synthetic protein conjugate. The kit of the invention has the advantages of simple pretreatment, easy operation, and is suitable for on-site, fast, and large-volume sample detection. The kit has low cost and low price, and the detection limit of the kit is less than 15ng/ml.

A process for removing formaldehyde from synthetic protein fibres to prevent the damage of residual formaldehyde to human body includes such steps as preparing 40% aqueous solution of sodium hydrogensulfite, washing the acetalized protein fibres with water, immersing in said aqueous solution at 40-60 deg.C for 5-10 min, water washing and centrifugal drying. Its advantages are high effect, simpleprocess, and bleaching action.

The invention relates to antibacterial recombination polypeptide and an expression vector of the antibacterial recombination polypeptide. The problems of being trivial in production technology, high in cost, low in yield and not suitable for mass production in an existing method of artificially synthesizing protein peptide are solved. According to the existing method of artificially synthesizing protein peptide, by the adoption of the method, a chimeric-structure peptide chain (N-FKCRRWQWRMKKLG-K-RSKNKGFKEQAKSLLKWILD-N) of Lactoferampin (Lfcin) and Lactoferampin (Lfampin) is synthesized. The gene nucleotide sequence of the antibacterial recombination polypeptide is shown as SEQ ID NO: 1 in a sequence table, and the amino acid sequence of the antibacterial recombination polypeptide is shown as SEQ ID NO: 2 in the sequence table. The antibacterial recombination polypeptide and the expression vector of the antibacterial recombination polypeptide have the advantages of being good in antibacterial effect, simple in production technology, low in cost, high in yield and suitable for mass production.

The present invention discloses a nano-silver synthetic protein derived from lysinibacillus sphaericus, also discloses a specific sequence of the nano-silver synthetic protein, discloses a synthesis mechanism for preparing nano-silver from the lysinibacillus sphaericus, and successfully amplifies corresponding DNA. A large number of bacteria with capacity of efficiently synthesizing the biologicalnano-silver can be prepared through recombinant vectors and prokaryotic expression, and the nano-silver synthetic protein has extremely wide application prospects and industrial value.

The present invention provides a protein synthesis method for efficiently synthesizing a desired protein so that it is properly folded so as to demonstrate a function thereof, the method comprising contacting a translation system with a solid phase-immobilized mRNA in which mRNA encoding that protein is immobilized on a solid phase, a protein synthesis apparatus for the method or the like. The protein synthesis method, protein synthesis apparatus or the like of the present invention are useful for, for example, large-volume synthesis of useful proteins.

The invention relates to a detection technology of organochlorine pesticide DDT (dichlorodiphenyltrichloroethane) metabolites, specifically relates to an anti-organochlorine pesticide DDT metabolite 2,2-bis(4-chlorophenyl)acetic acid (DDA) polyclonal antiserum prepared by synthesizing an antigen through protein coupling, and relates to a preparation method of a rapid detection kit capable of analyzing the DDA content in an environmental sample by utilizing the binding of a polyclonal antibody and a DDA antigen. An enzyme-linked immunosorbent assay kit for the rapid detection of organochlorine pesticide DDT metabolite 2,2-bis (4-chlorophenyl)acetic acid comprises a 96-well enzyme label plate detachable slat, an enzyme-labeled second antibody, a Tween-20-containing buffer salt washing liquid, a substrate liquid and a stopping liquid. A synthetic protein conjugate coated antigen is coated on the 96-well enzyme label plate by utilizing the prepared anti-serum containing anti-DDA-specific polyclonal antibody. The kit provided by the invention has the advantages of simple pre-treatment, easy operation process, low cost and price and the like, is suitable for on-site, rapid and large-batch sample detection, and has a detection limit less than 21 ng/ml.

A method is provided for capillary stabilization and vascular regeneration in retinal tissue. Capillary regeneration is accomplished with a protein that is a truncated norrin protein (synthetic). The truncated norrin protein has a longer half-life in the eye than native wild norrin proteins. Specific versions of the truncated norrin protein lack a cleavage site that an enzyme in the eye use to cleave to native norrin proteins and thereby shorten the useful life of the protein. The provided method encourages vascular development with an exogenous treatment of truncated norrin that have been investigated in oxygen-induced retinopathy (OIR) mice. The therapeutic feasibility of intravitreal injection of the norrin protein and its effect on retinal development by activating Wnt-signaling has also been shown.