Method for quickly tissue culture reproducing using ciliate desert-grass spore

A technology of centipede grass and grass spores is applied in the field of tissue culture and seedling propagation by using centipede grass spores, and can solve the problems of limited number of underground stems, death of centipede grass and the like

Inactive Publication Date: 2007-05-02
INST OF GEOGRAPHICAL SCI & NATURAL RESOURCE RES CAS
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Problems solved by technology

Although Yin Huaiyue ("Journal of Sichuan Normal University", 1991, the 12th volume 20-22 pages) has reported the tissue culture method of relevant centipede grass, we have carried out many experiments by the method of its report, but have not been successful all the time
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Method used

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  • Method for quickly tissue culture reproducing using ciliate desert-grass spore

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Experimental program
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Effect test

Embodiment 1

[0020] From the mature sporophyte of Centipede grass (Chenzhou, Hunan Province), the sporangia were brown, and the spores from the 6th to 41st pinnae at the top of the leaf were taken from top to bottom. A schematic diagram of the sampling location and photos of spores are shown in Figure 1.

[0021] Mature spores were sterilized with 70% ethanol for 1.5 min followed by 0.1% HgCl 2 The solution was sterilized for 3 minutes and rinsed 6 times with sterile water, and the spore microorganism contamination rate was less than 10%. Seed spores to 1 / 2 Knop + 0.5% CaCO 3 +1% sucrose medium, placed in a light incubator with a temperature of 25°C and a light intensity of 1000 lux, and the light time is 16 hours a day. After 60 days of culture, the spores germinate into prothallus and grow into the desired Aseptic sporophyte plants, the proportion of prothallus producing sporophyte plants can reach 56%. The spore plant production rate of other formula mediums is not as good as this fo...

Embodiment 2

[0032] The operation is the same as that of Example 1, and the medium for inducing callus in step 2 is MS+KT 4.6 μmol / L+NAA 0.1 μmol / L+0.7% agar+3% sucrose. The rooting medium used in step 4 was 1 / 2 MS+KT 0.5 μmol / L+NAA 2.7 μmol / L+0.7% agar+1% sucrose. 10 spores were inoculated, and after one subculture, 79 sporophyte plants were obtained, with an average of 7.9 sporophyte plants per spore.

Embodiment 3

[0034]The tissue culture seedlings bred in Example 1 were taken. After more than 200 days of storage, these seedlings still maintained vigorous vigor. Through a series of cultivation such as transfer and proliferation, the seedlings can be successfully transplanted into the field and survive after training. After being transplanted into the soil containing 507mg / kg of arsenic and cultivated for 100 days, the content of arsenic in the shoots can be as high as 3800mg / kg. The variation range of arsenic content in Centipede grass plants in the spore producing area is 3000-5000 mg / kg. This confirmed that the arsenic-accumulating ability of centipede grass was not degraded during long-term storage. This means that when the centipede grass is used to remediate large-area arsenic-contaminated soil, the present invention can be used to provide enough centipede grass seedling sources. In addition, the method of the present invention is not limited by natural conditions, regional diffe...

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Abstract

A tissue culture method of the spores of ciliate desert-grass for fast reproducing it in order to repair the As-polluted soil includes such steps as taking the mature spores of the ciliate desert-grass, culturing them in the culture medium prepared from MS, KT and NAA for inducing germination, secondary culturing in the culture medium prepared from MS, KT, NAA, agar and cane sugar, or from Knop and NAA for inducing rooting, pratising seedlings, and culturing the seedlings.

Description

technical field [0001] The invention relates to a method for plant tissue culture and germplasm preservation, and more particularly to a method for tissue culture and seedling propagation by utilizing spores of Centipede grass. Background technique [0002] Arsenic pollution is a very common and serious environmental problem at home and abroad. Chen Tongbin et al. found that the centipede grass (Pteris vittata L., Pteris genus) has a strong resistance to arsenic poisoning, and can enrich arsenic from arsenic-contaminated soil and accumulate it in the aboveground, so that it can be used to utilize its arsenic. Remediation and treatment of arsenic-contaminated soil (China Invention Patent Publication No.: CN1397390A, Chen Tongbin et al., "Science Bulletin", Vol. 47, No. 3, 2002, pp. 207-210). In addition, centipede grass can also be used as a flower crop for the production of fresh cut flowers. [0003] Centipede grass spores are very small, and their resistance to the outsi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈同斌莫良玉廖晓勇雷梅黄泽春
Owner INST OF GEOGRAPHICAL SCI & NATURAL RESOURCE RES CAS
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