105 results about "Gold core" patented technology

The invention relates to an island-shaped porous tri-metal nano rod with a gold core / silver-platinum alloy shell structure, which adopts the gold core / silver-platinum alloy shell structure formed by a cylindrical gold nano rod core and an island-shaped porous silver-platinum alloy shell coated on the outer surface of the cylindrical gold nano rod core. The preparation method comprises the following steps of preparation of gold crystal seed solution, preparation and purification of gold nano rod solution, preparation of solution of a platinum coated gold core / platinum shell nano rod, preparation of island-shaped porous tri-metal nano rod with the gold core / silver-platinum alloy shell structure and the like. The tri-metal nano rod has the advantages of stronger ability of catalyzing the methanol oxidation, stronger CO poisoning resistant ability, lower cost and the like, and can be widely used for preparing a methanol fuel battery catalyst; and the preparation method has the advantages of simplicity, low consumption, environmental protection, and high efficiency, and by the method, the island-shaped porous tri-metal nano rod with the gold core / silver-platinum alloy shell structure with high yield and narrow size distribution can be obtained.

The invention provides a dendritic gold-core / platinum-shell bimetal nanometer rod and a preparation method thereof. The dendritic gold-core / platinum-shell bimetal nanometer rod comprises a cylindrical gold nanometer-rod core and a porous platinum-shell layer covering the outer surface of the cylindrical gold nanometer-rod core; the diameter of the cylindrical gold nanometer-rod core is 10 to 15 nanometers; the length of the cylindrical gold nanometer-rod core is 55 to 60 nanometers; the thickness of the porous platinum-shell layer is 0.4 to 8 nanometers; and the length of platinum grains covering the outside of a nanometer rod is 2 to 4 nanometers. The preparation method provided by the invention has the advantages of simplicity, flexibility, environmental protection, green chemistry and low energy consumption. In addition, the obtained bimetal nanometer rod with a gold-core / platinum-shell structure has the characteristics of high yield, narrow size distribution, stable structure and adjustable optical properties.

The present invention provides a rectangular bimetallic nano rod monocrystal with gold-core / palladium shell and its preparation method. It includes a cylindrical gold nano rod internal core and a rectangular palladium shell layer which can be used for covering external surface of said cylindrical gold nano rod internal core. The diameter of the described cylindrical gold nano rod internal core is 10-15nm, and its length is 45-55nm; and the length of said rectangular palladium shell layer is 50-62nm, its width is 14-24nm and its height is 14-24nm.

Magnetic-optical iron oxide-gold core-shell nanoparticles are disclosed. Methods for making and using the nanoparticles are also disclosed.

The making of titania hollow ball with nanometer grains features int eh making of carbon balls with gold core through high temperature seal conditions, picking out 1 g of the said carbon ball to scatter inside the water free ethandiol or glycerin in 2.5-20g / L, dropping 30-500 micro litter aqua fortis of 0.01-2 m / l, then butyl titanate 500-5000 micro litter in 0.1-3m / l in the speed of 10-100 micro litter per minute, flowing in the silicon oil for 60-180 minutes, depositing in 600-800deg.C and calcining for 60-200 mintues. The sealed hollow ball has seal volume providing the movement of the gold core. Size of the hollow ball inside gold core and shell thickness can be adjusted.

The invention discloses a beneficiation method for high-carbon micro-fine particle sub-microscopic gold ore, and belongs to the technical field of mineral processing. Roasted ore, namely, concentrated gold ore, is obtained by performing flotation after gold ore is ground, grinding and selecting the floated concentrated gold ore again, and performing oxidizing roasting on the floated concentrated gold ore. The content of carbon and sulphur in the floated concentrated gold ore is high; and when iron pyrite in the floated concentrated gold ore is enriched to a certain degree, the gold grade is difficult to improve. The concentrated gold core with high quality can only be obtained through the oxidizing roasting. Two-section oxidizing roasting is performed on floated tailings so as to prevent over-roasting or under-roasting, and a sulphide which coats the gold is damaged through roasting so that cracks appear on a gold-loaded mineral structure to inhibit negative impact of organic carbon on subsequent cyaniding gold extracting process; and therefore, the purpose of remarkably improving the leading rate of gold is achieved. The method has the characteristics of low production cost, high gold recovery rate, high adaptability, wide application range and the like; and recycling and utilization of the ore are realized.

The ore separating method for raising the recovering rate of low-grade oxide gold and with high mud content and containnig coarse gold grain has the three kinds of technological process of heap leaching, gravitational separation and charcoal leaching combined. After crushing, washing ore, sieving, secondary crushing and twice grading, the coarse one grains enter heap leaching system directly white the fine ore grains enter the charcoal leaching system; preliminary vibration chute is set before the grading machine to retain coarse gold grains via gravitational separation. The said method has gold core recovering rate obviously higher than that of single heap leaching method and can utilize the precious resource fully.

The invention relates to a stabilized and ecological remediation comprehensive treatment method of slag heap of acidified gold ore containing heavy metal, and belongs to the technical field of heavy metal pollution treatment in an ore field. The method comprises the following steps: adding a certain amount of lime, coal ash and organic sludge compost into 0-20cm soil at the upper layer of the slag heap of the gold ore containing heavy metal, mixing the ore slag and the additive according to a certain ratio and stirring so as to mix evenly, adding water into the ore slag, pouring, maintaining at the natural condition for 15-30 days so as to stabilize the slag heap of the gold core containing heavy metal; then, planting heavy metal tolerant plants including ciliate desert-grass, ramie and vetiver grass, so as to further remediate the heavy metal polluted slag heap ecologically. The method can effectively stabilize heavy metal, such as arsenic and cadmium in the gold ore slag, neutralize acid in the ore slag, improve the pH of soil in the ore field, reduce the leaching of heavy metal, enhance the soil structure, increase the soil fertility, and achieve the ecological recovery of the heavy metal polluted ore slag field.

The invention discloses a surface plasma resonance probe with a silver-gold core-satellite structure and a preparation method thereof. The preparation method comprises the steps of: firstly, sequentially and quickly adding a glucose solution and a hexadecyl trimethyl ammonium bromide solution into a quantitative silver ammonia solution for reacting to prepare a nano silver cube solution; and then, standing a clean glass substrate in the nano silver cube solution to prepare a nano silver cube glass substrate; fixing single stranded DNA molecules on the prepared nano silver cube glass substrate; hybridizing the single stranded DNA with a miRNA molecule; and finally, preparing gold nanoparticles and decorating the gold nanoparticles at one end of the miRNA molecule to prepare the surface plasma resonance probe with a silver-gold core-satellite structure and capable of being applied in early detection of lung cancer. The particle diameter of the prepared surface plasma resonance probe is 50-70nm, the scattered spectrum peak is located at 450-650nm and the detection sensitivity is as high as 10nM.

The invention discloses an ultra-sensitive detection method of ochratoxin A based on a gold core-silver satellite chiral assembly body, and belongs to the field of analytical chemistry. The method mainly comprises the following steps: (1) synthesizing gold nanoparticles with the particle size of 35 nm; (2) synthesizing silver nanoparticles with the particle size of 8 nm; (3) modifying aptamers on the gold nanoparticles; (4) modifying part of complementary sequences of aptamers on the silver nanoparticles; (5) assembling a gold core silver satellite structure; (6) performing circular dichroism spectrum detection, and creating a standard curve of gold and silver chiral signal superposition and ochratoxin A concentration. The invention provides a method for detecting ochratoxin A by a chiral signal of the gold core silver satellite structure. Compared with a conventional detection method, the method provided by the invention is low in cost, high in sensitivity, convenient and quick, and has a very good practical application prospect.

The invention discloses a method for preparing magnetic metal-metal core-shell nano particles, which relates to metal nano particles. The method comprises: dissolving chloroauric acid and triphenylphosphine in ethanol respectively, filtering, cleaning, drying, obtaining an organic compound formed by gold and triphenylphosphine, mixing the organic compound with alkylamine, obtaining solution A, mixing an organic solvent A with a noble metal salt, adding mixed solution A, rising the temperature to perform a reaction, cooling and obtaining magnetic metal nano particles; dissolving the obtained organic compound formed by gold and triphenylphosphine in an organic solvent B, injecting obtained magnetic metal nano particles, keeping the temperature between 120 and 160 DEG C for 20 minutes to 1 hour, reducing to obtain gold and wrapping gold on the formed magnetic metal nano particles and obtaining nano particles in which gold and magnetic metal are in a core-shell structure; and washing the nano particles in which gold and magnetic metal are in a core-shell structure, performing centrifugal separation, drying and obtaining powder product, namely magnetic metal-gold core-shell nano particles.

The invention discloses an electrochemiluminescence immunoassay sensor for detecting a tumor marker. A preparation method of the sensor comprises the following steps: synthesizing polyaniline on conducting glass by an electropolymerization method, preparing g-C3N4, a silver-gold core shell nanosphere or copper oxide or manganese dioxide by the existing method, connecting the g-C3N4 on the polyaniline by 1-(3-dimethylamino propyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide mixed solution used as coupling agent, modifying the primary antibody on the prepared conducting glass, coating the surface of zinc oxide by the silver-gold core shell nanosphere, modifying second antibody on the silver-gold core shell nanosphere coating the surface of the zinc oxide, and detecting by electrochemiluminescence. The sensor has strong specificity, high sensitivity, simplicity in operation, wide linearity range and low detection limit.

The synthesis process of hollow silica ball including nanometer noble metal particles includes the following steps: dropping water solution of chloroauric acid into water solution of saccharide to react at 160-200 deg.C for 4-20 hr to obtain 0.01-2.5g / L water solution of carbon ball with gold core; mixing with 12.5-312.5 mg / L water solution of chloroauric acid, chloroplatinic acid or palladium chloride; reflexing the mixture solution at 100-160 deg.C for 3-10; centrifugal drying to obtain carbon ball with 5-60 nm noble metal core; dispersing the carbon ball in mixture solution of alcohol water solution and ammonia water solution in certain ratio; dropping alcohol solution of ethyl silicate to obtain precipitate; and calcining the precipitate at 600-800 deg.C for over 60 min. The present invention has easy control of the size of metal core in the hollow ball structure.

The present invention relates to gold@zinc oxide core-shell heterogeneous nanoparticles having strong sunlight absorption property, and a preparation method thereof, wherein the core of the nanoparticle is octahedral gold nanoparticles, the shell layer component is polycrystalline zinc oxide nanoparticles, and the structure parameters of the scale, the zinc oxide shell layer thickness, the gold core size and the like can be effectively regulated respectively within 30-160 nm, 10-30 nm and 50-200 nm. The preparation steps comprise: sequentially adding a proper amount of deionized water, sodium borohydride, zinc nitrate and a sodium hydroxide aqueous solution to a pre-prepared octahedral gold nanoparticle colloid solution, carrying out a reaction for 5-100 min at a temperature of 0-100 DEG C, and centrifugating at a rotation speed of 3000-14500 rotation / min to obtain the gold@zinc oxide nanoparticles. According to the present invention, the gold@zinc oxide nanoparticles have important application valus in the fields of photocatalysis, pollutant degradation, environmental governance, and the like.

The invention discloses a method of constructing an SERS spectral probe for detecting a breast cancer marker EGFR phosphorylated tyrosine. A gold plate is adopted as a base plate, a nanoparticle of acore-shell structure is adopted as a substrate for SERS detection, and a sandwich-configuration probe molecule with high selectivity and sensitivity is developed to detect phosphorylation of the breast cancer marker EGFR tyrosine. The spectral intensity of an SERS at a biomolecule Raman quiet region peak position of 2221cm<-1> in the 4-MB Raman spectrum is adopted as an internal standard, intensity normalization treatment is carried out on a spectral signal of the labeled molecule 4-MBA, and the influence of a Raman signal in a biomolecule fingerprint region on quantitative detection can be avoided, so that a linear relationship between the concentration of an EGFR phosphorylated tyrosine solution and the SERS intensity is greatly improved, and the detection sensitivity of the SERS spectral probe is further improved. The 4-MB and the 4-MBA are located in a middle layer of a gold core and a silver shell, it is ensured that the nature, spatial structure, quantity and the like of the SERSspectral probe are not changed, and thus the reliability of data is ensured. The method has a great potential in the terms of ultrasensitive detection of cancer markers.

A nano-hydrogel drug carrier material with photosensitivity and temperature sensibility relates to a kind of hydrogel. The invention provides the nano-hydrogel drug carrier material with photosensitivity and temperature sensibility and a preparation method thereof. The nano-hydrogel drug carrier material has core-shell structure, the core is nano-gold core and the shell is poly(N-isopropylacrylamide) shell. The preparation method comprises the following steps: dispersing nano-gold particles in water solution, adding acrylamide to obtain a suspending solution A, standing, centrifuging to collect precipitate, dispersing precipitate in deionized water again to obtain a suspending solution B, adding isopropylacrylamide, N,N'-methylenebisacrylamide, sodium lauryl sulfate and water-soluble drugto obtain a suspending solution C, stirring, adding ammonium persulphate to react and obtain a suspending solution D and finally collecting precipitate to obtain the nano-hydrogel drug carrier material with photosensitivity and temperature sensibility.

The invention relates to a preparation method of a hollow silicon-star gold core / shell nano material for wrapping adriamycin. The preparation method comprises the following steps of dissolving an HMSs (Hollow Mesoporous Silica Sphere), ultrasonically dispersing the dissolved HMSs, adding MPTMS (Mercaptopropyltrimethoxysilane) into the dispersed HMSs, introducing nitrogen into an obtained first mixture, refluxing the obtained first mixture in an oil bath, so as to obtain HMSs-SH (Hollow Mesoporous Silica Sphere-Sulfhydryl), afterwards, dissolving the HMSs-SH in water, adding an Au NPs (Aurum Nanoparticles) solution into an obtained second mixture, agitating the obtained second mixture, so as to obtain an HMSs / Au seed, dissolving the HMSs / Au seed in water, ultrasonically dispersing an obtained third mixture, adding a chloroauric acid solution into the dispersed third mixture, agitating an obtained fourth mixture, adding a silver nitrate solution and an ascorbic acid solution into the agitated fourth mixture, continuously agitating an obtained fifth mixture, so as to obtain an HMSs / Au NSs (Normal Saline Solution), dispersing the HMSs / Au NSs in water, adding an HS-PEG-RGD (Hassium-Polyethylene Glycol-Arginine-Glycine-Aspartic Acid) aqueous solution into an obtained sixth mixture, agitating an obtained seventh mixture, so as to obtain an HMSs / Au-PEG-RGD NSs, afterwards, dispersing the HMSs / Au-PEG-RGD NSs into ultrapure water, adding a DOX*HCL aqueous solution into an obtained eighth solution, agitating an obtained ninth mixture in a light-shielding manner, so that the hollow silicon-star gold core / shell nano material for wrapping the adriamycin is obtained. The nano material provided by the invention owns a specific targeting effect and excellent biocompatibility on a U87MG cell, has a synergistic enhancement effect on chemical therapy and photothermal therapy, and is wide in application prospect.

The invention provides a preparation method of a gold-copper sulfide nano material with a hollow core-shell structure and application thereof. The preparation method comprises the following steps of:mixing hexadecyl trimethyl ammonium bromide, chloroauric acid and sodium borohydride, and reacting to obtain gold seed solution; mixing the hexadecyl trimethyl ammonium bromide, silver nitrate, the chloroauric acid, ascorbic acid and the gold seed solution, and standing for growth to obtain a gold-core nano material; mixing polyvinylpyrrolidone, water, copper chloride and the gold-core nano material, and reacting to obtain a first reaction product; reacting the first reaction product with sodium hydroxide, reacting the obtained second reaction product with hydroxylamine hydrochloride, then reacting with sodium sulfide, centrifuging, cleaning obtained precipitates and then drying to obtain the gold-copper sulfide nano material with the hollow core-shell structure. The gold-copper sulfide nano material prepared by the preparation method has the advantages that under the irradiation of near-infrared light, the photothermal property of the copper sulfide is obviously improved by the plasmaresonance energy transfer mechanism of gold nano particles; the photodynamic property is higher; chemotherapeutic drugs also can be carried, and the tumor-cell killing efficiency is higher.

The invention discloses a method for preparing a carbon-supported high-activity gold or gold-platinum alloy or gold-core platinum-shell structural nano catalyst. The supporter of the catalyst is carbon black, and the active ingredient is gold or gold-platinum alloy or gold-core platinum-shell structural nano particles. The method is characterized by comprising the following steps of: modifying porous carbon by using a self-assembly method and using phi-phi bonds of a modifying chemical reagent to obtain a mercapto functionalized carbon black supporter, and depositing the gold or gold-platinum alloy or gold-core platinum-shell structural nano particles on the surface of the mercapto functionalized carbon black to obtain the gold or gold-platinum alloy or gold-core platinum-shell structural nano carbon-supported catalyst with high activity. The catalyst obtained by the method has high gold load factor; the load of the gold is controllable; the gold-carbon catalyst containing 1 to 64 mass percent of gold can be prepared; the catalyst has high activity, the size distribution of the gold nano particles is uniform and can be controlled, and the gold nano particles are connected with the porous carbon through the chemical bonds containing conductive benzene rings so as to enhance the interaction of the gold nano particles and the carbon; and the method has the advantages of low cost, simple process and low equipment requirement, and has good industrial application prospect.

The invention discloses a detection method for organophosphorus pesticides. The detection method comprises the following steps: fully mixing a test sample which may contain organophosphorus pesticides (such as thimet, omethoate and rogor) with gold-core platinum-shell nanoparticles and polyacrylic acid in a phosphate buffer solution so as to form a first mixed system; and then adding a sodium citrate buffer solution and the characteristic substrate of peroxidase, carrying out uniform mixing so as to obtain a second mixed system and determining the light absorption value of the obtained mixture in the range of visible-light wavebands so as to realize detection of the organophosphorus pesticides in the test sample. The invention also discloses a detection kit for the organophosphorus pesticides. The detection kit comprises the characteristic substrate of peroxidase, the gold-core platinum-shell nanoparticles, polyacrylic acid, auxiliary reagents and the like. The detection method for the organophosphorus pesticides in the invention is simple, convenient, rapid, low in cost, high in stability and applicable to detection of residual organophosphorus pesticides in environment samples, food samples and the like.

The invention provides a nano enzyme with a nanometer cage core of a hollow gold-platinum alloy and a shell of a porous silicon dioxide, a preparation method and an application thereof, and belongs tothe field of nanometer materials. The preparation method comprises the following steps: etching a gold core platinum shell nanostructure through potassium hexachloroplatinate to obtain a nanocage ofhollow gold-platinum alloy; coating silicon dioxide on the surface of the nanocage through tetraethyl silicate; and obtaining the nano enzyme taking the nanometer cage, in a round rod shape, of the hollow gold-platinum alloy as a core, and a silicon dioxide shell as the periphery of the core. The length of the core is 80 to 100 nm, and the diameter is 20 to 25 nm. The shell is provided with a channel of 3 to 5 nm. The nano enzyme is negatively charged. The antigen can be bound by an attractive effect of positive and negative electric charges to form a nano enzyme label antigen complex, which is used for performing enzyme linked immunoassay. According to the nano enzyme with the nanometer cage core of the hollow gold-platinum alloy and the shell of the porous silicon dioxide, the preparation method and the application thereof, the cost of a reagent of the natural enzyme label antigen complex is reduced, the working environment of enzyme linked immunodetection is extended, the defects that the natural enzyme is easy to deactivate and denature, etc., are overcame, and the nano enzyme can be widely applied to various enzyme linked immunodetection.

The invention discloses an Au@Au / Pt core-shell structure nano catalyst applied to alcohol fuel cells. The nano catalyst is in a concave cube shape, and the size is 29+ / -4nm; the inside of the nano catalyst is a gold core, and the outside of the nano catalyst is a gold / platinum shell. The catalyst is prepared by the following method which comprises the steps: firstly, preparing gold nanoparticles;then depositing silver on the surfaces of the gold nanoparticles to form an Au@Ag core-shell structure nano cube; then utilizing an in-situ growth method to prepare the Au@Ag / Au core-shell structure nano cube; finally, adding chloroplatinic acid to displace silver out to obtain a product. According to the catalyst disclosed by the invention, a pure platinum shell is changed into a shell prepared from platinum and gold jointly, so that platinum loading capacity is further reduced, and cost is reduced; meanwhile, the catalyst has an unique core-shell structure, has richer atom distribution and larger surface area, further interacts with the gold core to achieve a Plasmon resonance effect and can effectively enhance catalytic activity and weaken a poisonous effect; to alcohol oxidization reaction under the alkali condition, the nano catalyst has the advantages of better stability and catalytic activity.

The invention relates to a gold-core silver-shell nanometer composite material and preparation thereof. The composite material comprises bi-conical gold nanometer particles and rod-shaped silver nanometer particles wrapped at the external parts of the gold nanometer particles, and the mass ratio of the gold nanometer particles to the silver nanometer particles is (0.8-1):(0.77-3.75). Compared withthe prior art, according to the synthesized Au NBP@Ag NRs shell core nanometer material, the rod-shaped silver nanometer shells are directly grown on the mono-dispersed AU NBPs nanometer material with a tip, it is ensured that the synthesized nanometer composite material has good mono-dispersibility and adjustable diameter-length ratio, and the application range of the nanometer composite material is extended.

The invention discloses a beneficiation method to increase production indexes of carbonaceous gold ore and reduce influence of production backwater upon production indexes and belongs to the technicalfield of ore processing. The beneficiation method herein includes: performing wet grinding, adding the conditioner, sodium hydroxide, and the water purifier MFA, and performing pulping; adjusting post-grinding pulp to suitable concentration, adding an activator, a collector and a foamer to carry out quick floatation so as to obtain gold concentrate 1 and coarse tailings; adding the water purifierMFA to grind the coarse tailings again, and performing secondary floatation to obtain gold concentrate 2 and tailings, wherein the gold concentrate 1 and gold concentrate are used as qualified finished gold concentrate. The beneficiation method herein has the advantages that part of high-grade gold ore are beneficiated early and fast through quick floatation, the circulation quantity of middlingsis reduced, and the gold core can be maximally recycled during floatation; the beneficiation method has good technical flow and high applicability, the influence of backwater upon production indexesis relieved, and gold recovery rate is increased.

The invention discloses a graphene supported micron-gold core-shell structure and a preparation method thereof. The preparation method comprises the following steps: (1) obtaining polystyrene microsphere emulsion and adding a modified solution into the polystyrene microsphere emulsion; stirring to obtain surface modified polystyrene microsphere emulsion; (2) obtaining a gel solution of microsphere gold seeds; (3) obtaining a gel solution of polystyrene microspheres with gold shell layers on surfaces; and (4) drying the gel solution of the polystyrene microspheres with the gold shell layers on the surfaces to obtain a powdered sample, and immersing the sample in a tetrahydrofuran solution to form a plurality of layers of graphene on inner walls of the gold shell layers and remove the polystyrene microspheres, and drying to obtain the graphene supported micron-gold core-shell structure. According to the graphene supported micron-gold core-shell structure, the grain diameter of the polystyrene microspheres prepared by an improved mono-dispersion method can be controlled to be 4 microns to 10 microns, and the thickness of the graphene multilayered supported gold shell layers can be controlled to be dozens or hundreds nanometers.

The invention provides a colorimetric detection method of iodide ions and relates to iodide ion detection. The colorimetric detection method of the iodide ions comprises the following steps: dissolving HAuCl4 in water to prepare a HAuCl4 solution, and heating the HAuCl4 solution until boiling to obtain a solution A; dissolving sodium citrate in water and heating the solution until boiling to obtain a solution B; mixing the solution A with the solution B, heating the mixed solution so that the solution turns to wine red from light yellow, cooling until the temperature reaches a room temperature, thereby obtaining a gold nanoparticle solution, adding water to the gold nanoparticle solution and then orderly adding a silver-ammonia solution and formaldehyde to generate a reaction until the solution turns to yellow, thereby obtaining a gold-core and silver-shell nanoparticle solution; mixing the gold-core and silver-shell nanoparticle solution with a copper ion solution and applying to the mixed solution to the colorimetric detection of iodide ions. The colorimetric detection method of the iodide ions based on the gold-core and silver-shell nanoparticles and the copper ions as probes has the advantages of simplicity and convenience in operation, high reaction speed, high sensitivity, good specificity, fast semi-quantitative determination achievable by use of color change on the concentration of the iodide ions, and the like.

The invention relates to an optical fiber hydrogen sensor based on a core / shell nano periodic linear array plasma metamaterial. The sensor comprises an optical fiber and a gold core / palladium shell nanowire array patterned on an end surface of the optical fiber. The preparation method comprises steps that gold and aluminum are deposited on the end surface of the optical fiber, a porous aluminum oxide structure is formed through the anodic oxidation process, the diameter of a hole is corroded and enlarged, gold is filled, a gold nanowire is formed, an aluminum oxide gold nanowire composite filmis formed by the gold nanowire and the aluminum oxide structure, and palladium is plated around the gold nanowire or on an end surface of the gold nanowire to form a gold-palladium composite structure; a layer of periodically ordered polystyrene nano microsphere film is transferred on a surface of the palladium-plated aluminum oxide gold nanowire composite film, the gold-palladium composite nanowire structure which is not masked by the polystyrene nano microspheres is removed through ion etching, and the polystyrene nano microspheres are removed, and lastly, the alumina matrix is removed. Themethod is advantaged in that an optical fiber sensor is manufactured through the electrochemical technology, the method is simple and low in cost, and the optical fiber sensor has high sensitivity and high safety.

The invention discloses a visualized immunoassay method based on nanogold growth, which comprises the following steps: (1) adding antigens that have different concentrations and are capable of combining with specificity of an elisa plate into the elisa plate that is coated with a target acquisition antibody; (2) adding an enzyme labeled detection antibody into the elisa plate; (3) adding ascorbic acid ultraphosphate ester into the elisa plate, so that the ascorbic acid ultraphosphate ester reacts with labeled enzyme to generate ascorbic acid; (4) adding 20muL of the obtained reaction liquid to 220muL of gold rod growth liquid and then adding 10muL of gold core liquid, and after reaction, using a microplate reader for measuring absorption spectrum, and using a digital camera for recording the liquid color; (5) processing data and drawing a work curve; and (6) detecting and simulating a sample to be detected according to the above experimental steps. The method has the advantages that the operation is simple, the applicability is wide, the reaction is fast, various color changes can be generated, and the method can realize visualization quantitative determination for a target object.

The invention discloses a universal type multi-signal output biosensor, as well as a preparation method and application thereof. The universal type multi-signal output biosensor comprises two parts: a transcyclooctene modified vancomycin derivative used as a recognition molecule and functionalized gold and silver compound nanoparticles used as a signal conversion element, wherein the amino activated locus of the vancomycin molecule is covalently coupled with transcyclooctene; the gold and silver compound nanoparticles adopt silver shell-gold core compound nanoparticles; the surfaces of the gold and silver compound nanoparticles are simultaneously modified with a disulfide bond-containing tetrazine compound and a Raman microprobe molecule. The universal type multi-signal output biosensor can be applied to detection of Gram-positive pathogens, has the advantages of being high in efficiency, low in external environment influence, wide-spectral in targets and the like, and can meet the requirement of actual production and living.

The invention discloses a polypeptide nano hybrid as well as a preparation method and an application thereof; the general formula of the hybrid is [Au-S-S-peptide]n; the preparation method of the hybrid comprises the following steps: selecting a cysteine-modified polypeptide antagonist, copolymerizing sulfydryl-containing polypeptide and Au ions through domino reaction to generate an Au-polypeptide precursor, and then adding a prefabricated ultra-small gold core, further performing surface reduction through a reduction environment of the solution, and obtaining the polypeptide nano hybrid after reaction equilibrium is achieved. The hybrid is used for inhibiting the growth of tumors; according to the polypeptide nano hybrid obtained by the invention, the interaction of intracellular proteins in tumor cellsand proteinscan be regulated by inhibiting or destroying the mutual combination between beta-catenin and Bcl9, and finally the growth of tumors is inhibited; and the polypeptide nano hybrid prepared by the preparation method disclosed by the invention has the advantages of excellent solution stability and large drug loading capacity.