37 results about "Gold core" patented technology

The invention belongs to the field of antibiotic detection and biosensors, and particularly relates to a colorimetric array sensor for distinguishing different types of antibiotics based on platinum-based nano-enzyme and a detection method of the colorimetric array sensor. The colorimetric array sensor is composed of platinum nano-enzymes with three different structures, namely platinum nano-particles PtNPs, platinum nano-flowers PtNFs and gold-core platinum-shell nano-particles Au (at) PtNPs. According to the antibiotic detection method based on the platinum nano-enzyme colorimetric array sensing system, the peroxidase-like activity of the antibiotics is regulated and controlled through the non-specific interaction of the three platinum nano-enzymes (PtNPs, PtNFs and Au-coated PtNPs) and the antibiotics, a unique'fingerprint 'map is generated in combination with TMB-H2O2 chromogenic reaction, and efficient distinguishing and concentration detection of various antibiotics are achieved.

The invention discloses a free testosterone detection kit based on SERS (Surface Enhanced Raman Scattering) as well as a preparation method and a detection method of the kit. The kit consists of an SERS nano-label based on a gold core and a gold shell and functionalized magnetic beads, the structure of the SERS nano-label based on the gold core and the gold shell comprises the gold core, a Raman dye modified on the surface of the gold core, the gold shell coated on the surface, a sulfydryl polyethylene glycol carboxyl (HS-PEG-COOH) and sulfydryl polyethylene glycol (PEG-SH) protective layer outside the gold shell, and a free testosterone antigen. The method is simple and convenient to operate, can be used for analyzing trace free testosterone in a sample, has the advantages in the aspects of simplicity and convenience in operation, detection period, analysis cost and the like, and has important significance on rapid diagnosis of diseases caused by abnormal content of free testosterone.

The invention relates to the field of processing of transparent conductive films, and particularly discloses a preparation method of an ultrahigh-electrical-stability silver-gold core-shell nanowire transparent conductive film, which comprises the following steps: preparing a precursor liquid A from a gold source aqueous solution and a sulfite solution; uniformly mixing water, polyvinylpyrrolidone, a reducing agent and the alcohol dispersion liquid of the silver nanowires to obtain a reaction solution B; mixing the precursor solution A with the reaction solution B to prepare a silver-gold core-shell nanowire dispersion liquid; uniformly mixing the silver-gold core-shell nanowire dispersion liquid, a binder, a leveling agent and a solvent to obtain silver-gold core-shell nanowire conductive ink; and uniformly coating a substrate with the silver-gold core-shell nanowire conductive ink to obtain the silver-gold core-shell nanowire conductive thin film. The silver-gold core-shell nanowire conductive film can be used for preparing a silver-gold core-shell nanowire conductive film conductive electrode.

The invention discloses a ferroferric oxide, gold, internal label and gold core-shell nanoparticle and a preparation method thereof. Ferroferric oxide is used as a main body, nano-particles which take 16-20 nm gold nano-particles as cores, take sulfydryl molecules with benzene rings as internal standards and have the gold shell thickness of 35-40 nm are adsorbed on the surface of the ferroferric oxide, the overall particle size of the ferroferric oxide nano-particles is 200-460 nm, and the particle size of ferroferric oxide balls is 160-400 nm; the method comprises the following steps: uniformly dissolving ferric chloride, sodium acetate and polyethylene glycol in ethylene glycol according to a mass ratio of (1-2.7): (0.15-0.72): (0.5-2.3), heating to 200 DEG C, keeping the temperature for 8-72 hours, and naturally cooling to room temperature; and putting the product into a drying box, and drying at 60 DEG C. The method comprises the following steps: firstly, preparing Fe3O4 nano-particles, then carrying out surface modification on the Fe3O4 nano-particles, adsorbing 16nm gold seeds to the surfaces of the Fe3O4 nano-particles, adding internal standard molecules, and finally adding ascorbic acid and chloroauric acid to realize gold shell growth on the surfaces of the 16nm gold seeds. The material has the advantages of magnetic aggregation, easiness in separation and the like, and can be applied to the fields of pesticide residue detection, catalytic degradation, environment detection and analysis and the like based on an SERS (Surface Enhanced Raman Scattering) technology.

The invention discloses an Aspergillus niger detection kit based on SERS (Surface Enhanced Raman Scattering) and a preparation and detection method thereof, the kit comprises an SERS nano-label, the structure of the SERS nano-label takes bionic gold nanoflower as a gold core, and the surface of the gold core is modified with a Raman reactive dye and an Aspergillus niger specific aptamer. The kit has high sensitivity, specificity, reproducibility, uniformity and stability, Raman spectrum can be collected through portable Raman spectrum equipment, the kit can be used for analysis of trace Aspergillus niger in a sample, and the detection limit is lower than 10 CFU / mL. Compared with a traditional bacterial culture method, the method has the advantages in the aspects of simplicity and convenience in operation, detection period, analysis cost and the like. The method is suitable for food safety monitoring.

The application discloses an aspergillus niger detection kit based on SERS and a preparation and detection method thereof. The kit comprises a SERS nanolabel, and the structure of the SERS nanolabel is that a bionic gold nanoflower is used as a gold core, and a Raman active dye and aspergillus niger specific aptamer are modified on the surface of the gold core. The kit has high sensitivity, specificity, reproducibility, uniformity and stability, can collect Raman spectrum through a portable Raman spectrum equipment, can be used for analyzing trace aspergillus niger in a sample, and the detection limit is lower than 10 CFU / mL. Compared with a traditional bacterial culture method, the kit has advantages in operation simplicity, detection period and analysis cost. The kit is suitable for food safety monitoring.

The invention discloses a preparation method of silicon dioxide-gold core-shell nanoparticles, and belongs to the field of nano material preparation. The method comprises the following steps: preparing aminated silicon dioxide nanoparticles; preparing small gold nanocrystal seeds and adsorbing the small gold nanocrystal seeds on the surface of silicon dioxide; ascorbic acid or a salt thereof is used as a weak reducing agent, a chloroauric acid solution is slowly added, uniform growth of a gold shell is induced on the surface of a gold seed, and finally a complete core-shell structure is formed. According to the method, surface modification and adsorption are optimized, a weak reduction growth system is adopted, and growth kinetics is accurately controlled, so that the problems of uneven gold shell coating, complex process and poor reproducibility in a traditional method are effectively solved. The nanoparticles prepared by the method have the advantages of complete and smooth gold shell, accurate and controllable thickness (5-50nm), adjustable plasma resonance absorption, good monodispersity and the like, and have important application value in the fields of biomedicine and photoelectricity.

The invention discloses a preparation method of a multistage micro-nano structure composite material for nuclear radiation detection, and relates to the technical field of nuclear radiation detection. The preparation method comprises the following steps: modifying boron-containing molecules on the surface of a nanogold core by using a gold-sulfur self-assembly technology to obtain a functionalized nanogold core; growing a nano-gold shell by taking the nano-gold shell as a seed and chloroauric acid as a gold source; and finally, silver nitrate is used as a silver source, a nano silver shell is deposited in situ, and the multi-stage micro-nano structure composite material is obtained. According to the multi-stage micro-nano structure, gold nanoparticles serve as an inner core, boron-containing molecules serve as a neutron detection medium, a gold nanoshell serves as a surface-enhanced Raman scattering carrier, and a silver nanoshell serves as a gamma ray detection medium. The material has neutron detection and gamma ray detection capabilities, neutron response of boron-containing molecules and gamma ray response of the nano-silver shell can be quantitatively measured through SERS spectrum and LSPR spectrum respectively, high-efficiency discrimination of the dose of a mixed radiation field can be realized, and the material has a wide application prospect in the field of nuclear radiation detection.

The application discloses a spectrum shift type hydrogen sensor and a preparation method thereof, and comprises a silicon substrate, a suspended micro-bridge formed by local etching and releasing of the silicon substrate, and a nanowire array arranged on the suspended micro-bridge, wherein the nanowire sensitive structure in the nanowire array comprises, from inside to outside, a gold core, a palladium shell layer coated on the periphery of the gold core, an air gap located outside the palladium shell layer, an annular gold shell layer arranged around the air gap, and a porous alumina filling layer reserved in the final device and filled outside the annular gold shell layer; a temperature control circuit is connected with the suspended micro-bridge and used for controlling the working temperature of the suspended micro-bridge; wherein the nanowire sensitive structure is subjected to plasmonic resonance spectrum peak displacement under the action of hydrogen, and the air gap and the annular gold shell layer form a radial coupling cavity for amplifying the readout amplitude of the spectrum peak displacement. The application can realize high-sensitivity, fast-recovery and high-stability hydrogen spectrum detection.

The invention discloses a preparation method of a Ti3C2-PDDA-Au-coated AgNPs composite substrate material as well as a product and application of the Ti3C2-PDDA-Au-coated AgNPs composite substrate material, and belongs to the technical field of sensors. According to the invention, a Ti3C2 nanosheet and Au (at) AgNPs (gold core and silver shell nanoparticles) are prepared, and a Ti3C2-PDDA-Au (at) AgNPs composite substrate is constructed by using an electrostatic self-assembly method. The composite substrate can be assembled with probe molecules to form an SERS sensor, the sensor can rapidly and sensitively detect the plasticizer in the edible oil, the sensitivity is high, the detection limit is low, the linear relation is good, a novel efficient method is provided for detection of the plasticizer in the edible oil, and the SERS sensor has important practical value for guaranteeing food safety.

The invention relates to the technical field of medical detection, in particular to a nanoprobe for simultaneously detecting and inhibiting VCAM-1mRNA expression as well as a preparation method and application of the nanoprobe. The nano particle takes a nano Au particle as a core, and p-mercaptocyanophenyl (4-MBN) serving as a Raman signal internal standard molecule is modified on the surface of the Au core; the nano particle takes a gold layer as a shell, and a VCAM-1mRNA targeting recognition sequence segment is fixed on the Au shell; and the tail end of the VCAM-1 mRNA targeted recognition sequence segment is modified with a Raman reporter molecule Cy5. The recognition sequence segment on the nanoprobe can be specifically combined with VCAM-1mRNA to form a rigid double strand, so that Cy5 is far away from the surface of the gold core-shell nanoparticle, the SERS signal strength of Cy5 is weakened, the position of 4-MBN located on a spacing layer is unchanged, and the SERS signal of the 4-MBN is also unchanged. The expression of the VCAM-1 protein can be inhibited by the accompanying mRNA consumption in the detection process, so that the synchronous monitoring and treatment intervention of the atherosclerosis disease closely associated with the expression of the VCAM-1 are realized.

Disclosed are a pharmaceutical use of a gold cluster for the treatment of diabetes in a subject. The gold cluster comprises a gold core and a ligand modifying the gold core.

To provide fluorescence-enhancing nanoparticles that do not reduce the fluorescence intensity of fluorescent dyes, but rather further enhance the fluorescence intensity.SOLUTION: Provided are fluorescence-enhancing nanoparticles comprising core-shell nanoparticles formed of a core particle and a shell, at least one fluorescent dye, and a spacer located between the core-shell nanoparticles and the fluorescent dye, the fluorescent dye having an excitation wavelength and a fluorescence wavelength, the core-shell nanoparticles having a maximum wavelength of localized surface plasmon resonance, the core-shell nanoparticles being metal nanoparticles composed of a combination of a silver core particle and a gold shell or a combination of a gold core particle and a silver shell, and the spacer being single-stranded DNA.SELECTED DRAWING: Figure 1

The invention discloses a method for quantitatively detecting creatinine in serum by pH-assisted ratio type SERS (Surface Enhanced Raman Scattering), which comprises the following steps: preparing silver-coated gold core-shell nanoparticles Au (at) MPA (at) Ag NPs with internal standard thiohydracrylic acid as an SERS substrate, and normalizing an SERS signal of the creatinine by using an internal standard signal. In addition, the pH of the creatinine is adjusted to enhance the adsorption effect of the creatinine and the nanoparticles, so that a Raman signal is enhanced. The detection method disclosed by the invention has the advantages of effectively eliminating interference of environmental factors, non-uniformity of samples, high dependence on a substrate and the like. Moreover, 2.5 mol / L of creatinine can be detected by the creatinine detection method based on the pH-assisted ratio type SERS technology, the adding standard recovery rate in a serum sample is 98.1%-107.4%, the relative standard deviation is smaller than 5.8%, and the quantitative accuracy of creatinine detection is improved.

The invention provides a method for preparing inhalable targeted gold nanoparticles for accelerating pulmonary delivery and treating pulmonary inflammation. The method comprises the following steps: preparing plasmids; carrying out plasmid transformation; carrying out protein expression induction; carrying out protein purification; and the gold nanoparticles (Au (at) PEG-RBD NP) combined with the spikes RBD are prepared. Nanoparticles (NP) in air accumulate more readily in lungs with lipopolysaccharide-induced acute respiratory distress syndrome (ARDS) than in healthy lungs and enter alveolar epithelial cells that express angiotensin converting enzyme (ACE) 2 and L-SIGN (both are Spike receptors activated in ARDS hamsters). The nanoparticles (NP) treat tissue damage, oxidative stress, and inflammation more effectively than corticosteroids, and do not remain gold or create toxicity in the major organs one year after inhalation. The gold core can be used for inhibiting p38 alpha mitogen activated protein kinase and polo-like kinase 3. Similar therapeutic effects have been observed in hamsters suffering from hydrochloric acid induced ARDS. This self-therapeutic nanoparticle (NP), when combined with biomimetic and non-invasive delivery, provides a safe, efficient, and targeted treatment for lung inflammation.

A nanotag includes a gold core, the gold core is sequentially coated with a silver shell and a gold shell, and a Raman dye is arranged between the silver shell and the gold shell; further it discloses a making method for a high-stability SERS nanotag that includes the following steps: a. adding tetrachloroauric acid into ultrapure water, after boiling, quickly adding trisodium citrate, continuously boiling for a few minutes, stopping heating, and cooling; b. adding hexadecyl trimethyl ammonium chloride (CTAC) into product obtained in the step a, stirring, and then adding ascorbic acid and AgNO3, and centrifugating and resuspending in the CTAC; c. adding CTAC into product obtained in the step b, stirring, adding a Raman dye, ascorbic acid and sodium hydroxide, injecting a mixture of tetrachloroauric acid and potassium iodide, and centrifuge the mixture and resuspending in the CTAC.

Disclosed are a pharmaceutical use of a gold cluster for the treatment of diabetes in a subject. The gold cluster comprises a gold core and a ligand modifying the gold core.

This invention discloses a method for preparing Au@1,4-BDT@Au@4-MBA core-shell structured nanoparticles with sub-nanometer gaps. The method primarily uses a thiol molecule with a benzene ring as an internal standard, specifically 1,4-benzenedithiophenol (1,4-BDT), precisely encapsulated within the gap between a gold core and a gold shell. The gold core has a diameter of 35–40 nm, and the gold shell has a diameter of 45–60 nm, resulting in an overall particle size of 80–100 nm. The internal standard molecule located in the sub-nanometer gap layer generates a very strong electromagnetic enhancement signal, and because it is physically isolated by the outer gold shell layer, its signal is unaffected by external pH environments or solution matrices, providing an excellent reference. An external standard molecule (4-MBA) is then used for the outermost coating, serving as an acid-base sensing unit. A ratiometric calibration model is established based on the ratio of its characteristic peak to the internal standard peak, effectively reducing errors caused by uneven hotspots and matrix interference. This material can be applied to pH detection of human sweat based on SERS technology.

The invention relates to a glycosylated luminous gold nanomaterial as well as a preparation method and application thereof. The preparation method comprises the following steps: performing in-situ reduction on chloroauric acid by taking thiomalic acid (MSA) as a protective agent to obtain a gold nano-cluster of which the gold core particle size is about 1.8 nm; and activating carboxyl on the surface of the gold nano-cluster (GlcN-MSA-AuNPs) by EDC / NHS, and coupling with glucosamine to obtain the glucosamine-modified gold nano-cluster (GlcN-MSA-AuNPs). The nano material can generate stable fluorescence in a near-infrared region I (about 786 nm) under the excitation of 600 nm, can effectively reduce normal tissue accumulation and non-specific adsorption, and has good biocompatibility and tumor active targeting property. The nano material prepared by the invention can be used for rapid detection and near-infrared imaging of subcutaneous transplanted tumors of small animals.

The invention provides a nanoprobe with a polydopamine-gold core-shell structure as well as a preparation method and application of the nanoprobe, and relates to the technical field of lateral flow immunochromatography. The nanoprobe comprises a polydopamine inner core, a first gold shell layer and a second gold shell layer from inside to outside, wherein the surface of the polydopamine inner core is modified with polyethyleneimine; wherein the surface of the first gold shell layer is connected with a first Raman signal molecule DTNB; wherein the surface of the second gold shell layer is connected with a second Raman signal molecule DTNB, and the terminal carboxyl of the second Raman signal molecule DTNB is coupled with an anti-H1N1 monoclonal antibody. The nanoprobe with the polydopamine-gold core-shell structure has three functions of surface enhanced Raman scattering (SERS), photothermal conversion and visible signal output.

1. Name of the product in this design: Disposable sanitary substrate (gold core). 2. Application of this design: It can be used for disposable sanitary base sheets, such as the surface and back layers, core, front and back waistbands of products like baby diapers, adult diapers, baby panty-style diapers, adult panty-style diapers, incontinence pads, nursing pads, sanitary napkins, panty-style sanitary napkins, panty liners, maternity pads, and breast pads. It can also be used for products like wet wipes and pet pads. 3. The key design features of this product are the embossed pattern on the disposable sanitary substrate. 4. The image or photograph that best illustrates the design's key features: the front view. 5. This design is a two-dimensional product, and the bottom view, top view, left view, right view, and rear view are omitted. 6. This design is a planar product with continuous four sides and no defined boundaries.

The invention provides antibody functionalized Au and Raman signal molecule and Au nanoparticles, a sandwich structure formed by combining magnetic beads and nanoparticles and application of the sandwich structure, and belongs to the technical field of myocarditis related index detection. A Raman signal molecule is introduced into the surface of a gold nanoparticle, a gold shell layer is deposited, the surface of the gold shell layer is modified with an antibody, and the antibody functionalized Au and Raman signal molecule and Au nanoparticle is constructed. According to the invention, the silver-coated gold nanoparticles are prepared, silver on the outer layers of the silver-coated gold nanoparticles can be etched while the silver-coated gold nanoparticles and a second part of HAuCl4 are subjected to reduction reaction to coat a gold shell layer, and a nano cavity with extremely strong hot spots is formed between an inner-layer nano gold core and an outer-layer gold shell, so that amplification can be realized, and repeatable Raman signal reading can be realized; and accurate detection is promoted. The sandwich structure formed by further combining the magnetic beads and the nanoparticles can accurately and quickly realize Raman spectrum identification of five positive and negative samples of myocarditis.

A method for preparing inhalable targeted gold nanoparticles for accelerated lung delivery and treating lung inflammation is provided. The method includes preparing plasmids; performing plasmids transformation; performing protein expression induction; performing protein purification; and preparing spike RBD-conjugated gold nanoparticles (Au@PEG-RBD NPs). The airborne NP preferentially accumulates in lungs with lipopolysaccharides-induced ARDS to healthy lungs and enters alveolar epithelial cells that express angiotensin converting enzyme (ACE) 2 and L-SIGN, both Spike receptors activated in ARDS hamsters. The NP treats tissue injury, oxidative stress, and inflammation more effectively than corticosteroids, without gold retention in major organs or toxicity 1-year post-inhalation. The gold core blocks p38α mitogen-activated protein kinase and polo-like kinase 3. Similar efficacy is observed in hamsters with hydrochloric acid-induced ARDS. The self-therapeutic NPs, when combined with bioinspiration and non-invasive delivery, provide a safe, effective, and targeted treatment for lung inflammation.

The application discloses a kind of shell regulation discrete chiral gold core silver shell nanomaterial and its preparation method, method includes the following steps: using anisotropic gold nanorod material, into which adding surfactant, cysteine, ascorbic acid, chloroauric acid solution is reacted, discrete chiral gold nanorod with strong plasmonic circular dichroism response is obtained;Then after centrifugation and purification concentration, adding cysteine, silver nitrate, ascorbic acid, secondary reaction is carried out to chiral gold nanorod.The scheme reaction condition is mild, easy to operate;And the discrete chiral gold core silver shell nanoparticles obtained is very good, single nanoparticle, with strong PCD signal, and chiral silver shell can amplify and reverse the chirality of chiral gold core, very suitable for biological sensing, basic research (such as surface chemistry and PCD mechanism research) etc..

The application discloses a simple and rapid quantitative visual detection method for hexavalent chromium in water, which realizes etching reaction of gold core silver shell nanoparticles (Au@Ag NPs) and hexavalent chromium by adding hydrogen bromide solution and strictly controlling the amount of hydrogen bromide solution, and comprises the following steps: (1) preparing an Au@Ag NPs nanometer colorimetric probe solution; (2) configuring standard solutions of different concentrations of hexavalent chromium, adding the standard solutions of hexavalent chromium into a detection solution containing the Au@Ag NPs nanometer colorimetric probe solution and hydrogen bromide solution, and determining a blue channel intensity value I B of each standard solution according to a standard curve of hexavalent chromium and a detection limit; and (3) performing visual detection on actual water samples of hexavalent chromium. The detection method provided by the application has the advantages of mild detection conditions, good elimination of matrix interference, high stability and sensitivity, simple operation, rapid real-time quantification, low cost and wide application prospect.

The application provides a golden core particle image generation method and system based on multi-scale feature comparison, acquires multiple images of the same type of core particles that may have defect information through random image acquisition; through pose alignment and brightness adjustment, the multiple core particles are kept consistent in the physical space; through a pre-trained convolutional neural network model, multi-scale features are extracted, difference information of the region position is judged, and no difference regions are selected for content filling on the basis thereof; pixel-level information of a plurality of no difference regions is fused to generate pixel content of the final golden core particle; specifically, on the basis of a plurality of images that may contain defective core particles, the standard morphology of each position without defects is determined through the mutual relationship between the images, so that pixel-level synthesis of the golden core particle is realized, and effective reference basis is provided for a wafer defect detection process with a pattern.

The invention belongs to the field of portable and trace detection of pollutants, and particularly relates to an aM-level detection method of perfluorooctanoic acid. The method comprises the following steps: firstly, triggering enzyme-free catalysis hairpin assembly by using a PFOA aptamer to generate double-stranded DNA to activate a CRISPR-Cas12a system, so as to realize target cyclic amplification and signal transduction; a DNA signal is converted into a copper element signal through a gold-core copper-shell nanoparticle labeling strategy, and multi-stage signal amplification is achieved; and finally, quantifying a Cu signal at 327 nm by adopting a capillary tube-micro plasma emission spectrometer driven by a self-developed lithium battery. The detection limit of the platform to PFOA reaches 0.50 aM, which is 4 orders of magnitude lower than the American EPA standard; the linear range spans 6 orders of magnitude (2.5 aM-2.5 pM); the standard addition recovery rate of 6 kinds of complex environment water samples (landfill leachate, breeding wastewater and the like) and 3 kinds of dairy products is 95.54%-108.63% (RSDlt; 5%).

The invention discloses a method for label-free detection of tryptophan and kynurenine in serum based on a surface enhanced Raman scattering technology, and belongs to the field of Raman spectrum molecule SERS detection. The invention aims to solve the problems that the traditional Trp molecule and Kyn molecule detection method is expensive in equipment, tedious in operation, time-consuming in analysis and difficult to meet clinical instant detection requirements, and a blank still exists in the research field of directly and synchronously detecting Trp and Kyn molecules in serum based on an unmarked SERS (Surface Enhanced Raman Scattering) technology at present. The method comprises the following steps: 1, preparing gold-core tellurium-shell nanoparticles; 2, preparing an Au (at) TeNPs SERS substrate by a self-assembly method; and 3, detecting tryptophan and kynurenine molecules by SERS (Surface Enhanced Raman Scattering Based on the Au-coated TeNPs SERS substrate, synchronous and rapid SERS detection on Trp and Kyn molecules in a physiological concentration range in a serum medium is achieved for the first time, and the detection method is rapid and economical and has remarkable clinical application potential.

The application discloses gold nanoclusters and application thereof in detection of organophosphorus pesticide residues, wherein the gold nanoclusters comprise a gold core center structure, a first layer of ligand structure formed by 6-azido-2-thiothymine combined on the surface of the gold core through a gold-sulfur bond, a second layer of ligand structure formed by L-arginine combined with the 6-azido-2-thiothymine through a hydrogen bond, and a third layer of ligand structure formed by tri-methylphenyl ammonium iodide combined with the L-arginine through electrostatic force and by tri-methylphenyl ammonium iodide molecules combined through a pi-pi interaction force. The gold nanoclusters have high sensitivity to mercapto compounds and pH, a detection limit of chlorpyrifos of 0.02 micrograms per liter is achieved by using the detection method, the detection method has high sensitivity, and the detection method is simple, economical and rapid.

The invention discloses a preparation method of a bimetallic dual-network hydrogel SERS (Surface Enhanced Raman Scattering) sensor and application of the bimetallic dual-network hydrogel SERS sensor in detection of antitumor drugs, and belongs to the technical field of biological medicine detection. The preparation method of the hydrogel SERS sensor comprises the following steps: firstly, preparing gold nanoparticles (Au NPs), and then preparing silver-coated gold core-shell nanoparticles (Au-coated Ag NPs); nano-particles are concentrated and then are loaded in the agarose (AG) and sodium alginate (SA) hydrogel in proportion; and carboxylated cellulose (C-CNF) is added to stabilize nano-particles in the hydrogel. The Au NPs and the Au-coated Ag NPs are added at the same time, so that the SERS signal intensity of the hydrogel is higher than that of a single nanoparticle, and the SERS detection sensitivity is improved. The method provided by the invention can realize rapid detection of the antitumor drug in blood, does not need complex and processing steps, successfully realizes accurate identification of the antitumor drug, and has a wide prospect in clinical detection of drugs in blood.