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Preparation method of hollow silicon-star gold core/shell nano material for wrapping adriamycin

A technology of nanomaterials and doxorubicin, applied in medical preparations with non-active ingredients, medical preparations containing active ingredients, wave energy or particle radiation treatment materials, etc., can solve the problem that no hollow silicon-Venus core/shell has been found Nanomaterials report and other issues, to achieve good in vivo tumor treatment effect, simple preparation method, good in vivo targeted tumor treatment effect

Inactive Publication Date: 2016-12-07
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] A search of domestic and foreign literature and patents on nanomaterials for tumor combination therapy shows that there is no report on the preparation of DOX-wrapped hollow silicon-venus core / shell nanomaterials and its application in combination therapy of tumor chemotherapy and photothermal therapy.

Method used

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  • Preparation method of hollow silicon-star gold core/shell nano material for wrapping adriamycin
  • Preparation method of hollow silicon-star gold core/shell nano material for wrapping adriamycin
  • Preparation method of hollow silicon-star gold core/shell nano material for wrapping adriamycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The specific process of preparing HMSs / Au-PEG-RGD NSs / DOX nanomaterials is as follows figure 1 shown.

[0046] (1) Dissolve 100 mg of hollow mesoporous silica (HMSs) in 100 mL of isopropanol, and disperse it with ultrasound, and add 600 μL of (3-aminopropyl)dimethylethoxysilane (MPTMS) dropwise (concentration: 0.846g / mL), protected by nitrogen gas, refluxed in an oil bath at 70-80°C for 8h, centrifuged at 8500r / min, washed twice with ethanol and once with ultrapure water to obtain thiol-modified hollow mesoporous dioxide Silicon (HMSs-SH). DLS and TEM test results showed that the surface of hollow mesoporous silica was successfully modified with -SH( figure 2 ), the cavity diameter of HMSs-SH is 150mm, and the shell thickness is 20mm ( image 3 a 1 -a 3 ).

[0047] (2) Dissolve 30 mg of HMSs-SH obtained in (1) in 5 mL of water, and disperse with ultrasonic waves, add 1 mL of gold nanoparticle solution (concentration: 4 mg / mL) dropwise, stir at room temperature f...

Embodiment 2

[0052] Take the product obtained in (5) in Example 1 and configure it with a buffer solution of pH=7.4 and pH=5.0 to form a 1mg / mL solution, put 1mL of the solution into a dialysis bag and place it in a 50mL PE tube containing 9mL of the corresponding buffer solution , they were divided into two groups (one group was irradiated with 808nm laser for 5 min, and the other was not irradiated with laser), and then placed in a shaker at 37°C for oscillation. Then take 1mL of the liquid outside the dialysis bag after 1h, 2h, 3h, 4h, 5h, 6h and 8h respectively, measure its absorbance at 480nm with a UV-visible spectrophotometer, and then add 1mL of the corresponding solution to the outside of the dialysis bag. Buffer solution, wherein the test group (808nm laser irradiation group) needs to measure the absorbance at 480nm after 5 minutes of laser irradiation. The in vitro release curve test results of HMSs / Au NSs-PEG-RGD / DOX nanomaterials showed that under physiological environment (no...

Embodiment 3

[0054] Get the product obtained in (5) in Example 1 and use ultrapure water to prepare a mother solution with a gold concentration of 20mM, then gradiently dilute it to 10mM, 5mM and 1mM materials, and carry out photothermal conversion performance tests on a series of concentration materials under 808nm laser . Ultrapure water, HMSs and HMSs / Au seed were used as blank controls. The test results of the heating performance of HMSs / Au NSs-PEG-RGD / DOX nanomaterials show that: within the range of the test concentration, HMSs / Au NSs-PEG-RGD / DOX nanomaterials exhibit excellent photothermal conversion effect, and as the nanomaterials As the concentration increases, the temperature rises more obviously ( Figure 7 ).

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Abstract

The invention relates to a preparation method of a hollow silicon-star gold core / shell nano material for wrapping adriamycin. The preparation method comprises the following steps of dissolving an HMSs (Hollow Mesoporous Silica Sphere), ultrasonically dispersing the dissolved HMSs, adding MPTMS (Mercaptopropyltrimethoxysilane) into the dispersed HMSs, introducing nitrogen into an obtained first mixture, refluxing the obtained first mixture in an oil bath, so as to obtain HMSs-SH (Hollow Mesoporous Silica Sphere-Sulfhydryl), afterwards, dissolving the HMSs-SH in water, adding an Au NPs (Aurum Nanoparticles) solution into an obtained second mixture, agitating the obtained second mixture, so as to obtain an HMSs / Au seed, dissolving the HMSs / Au seed in water, ultrasonically dispersing an obtained third mixture, adding a chloroauric acid solution into the dispersed third mixture, agitating an obtained fourth mixture, adding a silver nitrate solution and an ascorbic acid solution into the agitated fourth mixture, continuously agitating an obtained fifth mixture, so as to obtain an HMSs / Au NSs (Normal Saline Solution), dispersing the HMSs / Au NSs in water, adding an HS-PEG-RGD (Hassium-Polyethylene Glycol-Arginine-Glycine-Aspartic Acid) aqueous solution into an obtained sixth mixture, agitating an obtained seventh mixture, so as to obtain an HMSs / Au-PEG-RGD NSs, afterwards, dispersing the HMSs / Au-PEG-RGD NSs into ultrapure water, adding a DOX*HCL aqueous solution into an obtained eighth solution, agitating an obtained ninth mixture in a light-shielding manner, so that the hollow silicon-star gold core / shell nano material for wrapping the adriamycin is obtained. The nano material provided by the invention owns a specific targeting effect and excellent biocompatibility on a U87MG cell, has a synergistic enhancement effect on chemical therapy and photothermal therapy, and is wide in application prospect.

Description

technical field [0001] The invention belongs to the field of preparation of core / shell structure nanomaterials, in particular to a preparation method of hollow silicon-venus core / shell nanomaterials wrapped with doxorubicin. Background technique [0002] At present, commonly used tumor treatment methods include chemotherapy, surgery, radiotherapy, photothermal therapy, biological immunotherapy and gene therapy. Among them, chemotherapy can treat more than ten kinds of tumors, and can kill not only some cancer cells but also metastatic cancer cells. It is a systemic therapeutic method. However, chemotherapy is non-specific, and while killing tumor cells, it also causes great damage to normal human cells. Photothermal therapy can achieve local tumor treatment, with good treatment specificity, short treatment time and obvious therapeutic effect, non-toxic and harmless, but photothermal therapy cannot go deep into the deep layer of the organism, and lacks effective means for in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/51A61K31/704A61K47/48A61K47/02A61K47/42A61K47/34A61P35/00
CPCA61K41/0052A61K9/5115A61K9/5146A61K9/5169A61K31/704
Inventor 史向阳李鑫韦平沈明武邢玲溪赵凌舟杜联芳
Owner DONGHUA UNIV
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