78results about How to "With industrial implementation" patented technology

The invention relates to a preparation method of pegylation modified hyperbranched poly(ethylene imine) (PEI) coated nano-gold particles, which comprises the following steps: modifying PEI by using mPEG (polyethylene glycol)-COOH, and sequentially carrying out dialysis and freeze-drying on the obtained product so as to obtain PEI-mPEG; taking the solid, dissolving the solid by using water, adding a HAuCl4 solution into the dissolved solid, stirring the obtained product, adding a NaBH4 solution into the obtained product, and carrying out reaction on the obtained mixture at room temperature; and adding triethylamine and acetic anhydride into the obtained object, and after the reaction is completed, carrying out dialysis and freezing on the obtained product so as to obtain pegylation modified hyperbranched polymine coated nano-gold particles. According to the invention, the cheap and easily-obtained PEI is taken as a carrier, so that the cost of materials is reduced; the surface of PEI is modified by using mPEG-COOH, so that the biocompatibility of materials and the colloidal stability of nano-gold particles are improved, and the nano-gold particles are successfully applied to vivo CT (computed tomography) imaging. The method disclosed by the invention is simple in design, mild in reaction conditions and easy to operate, and has an industrialized implementation prospect.

The invention relates to an in-situ preparation method for a functional nullvalent nanometer iron/polyelectrolyte composite fibrofelt. The method comprises the following steps: (1) preparing polyelectrolyte mixed solution with concentration between 7 and 12 percent by polyacrylic acid PAA, and preparing a nanometer fibrofelt according to a principle of electrostatic spinning; (2) carrying out heat treatment on the nanometer fibrofelt to prepare a water-fast polyelectrolyte fibrofelt; (3) preparing soluble iron salt solution, and preparing reducing agent solution with concentration 4 to 6 times of the iron salt solution; (4) dipping the (2) in the iron salt solution; (5) rinsing; (6) dripping the reducing agent solution onto the complex iron ion fibrofelt; (7) rinsing by deionized water; (8) drying and storing. In the fibrofelt prepared by the method, nanometer iron particles are dispersed evenly without aggregation phenomenon, and the fibrofelt can effectively fix nanometer iron particles, thereby ensuring effective recycle of the nanometer iron particles in practical application without secondary pollution.

The invention relates to a preparation method of a CT/MR bimodal imaging nano contrast medium with a folate targeting function. The method comprises the following steps of: (1) preparing FA-PEG-COOH; (2) modifying the dendrimer by use of DOTA-NHS, COOH-PEG-FA and mPEG-COOH to obtain the pegylation dendrimer modified by DOTA and folate; and (3) adding chloroauric acid solution, NaBH4 solution and Gd(NO3)3.6H2O into the dendrimer solution obtained in the step (2), then adding triethylamine and acetic anhydride, and after reaction, performing dialysis and freeze-drying. The preparation method provided by the invention is simple and easy to operate, and has mild reaction conditions and prospects for industrial implementation; and the CT/MR bimodal imaging nano contrast medium has better water solubility and stability, realizes a specific targeting function for the folate receptor high-expression cancer cell, and is expected to be applied to early detection of tumor.

The invention relates to a preparation of a gold nanoparticle coated with a folic acid-modified pegylated dendrimer. The method comprises the following steps of: (1) preparing a folic acid-modified polyethylene glycol solid product FA-PEG-COOH; (2) modifying G5.NH2 with FA-PAG-COOH and mPEG-COOH in sequence to obtain solid G5.NH2-(PEG-FA)-mPEG; and (3) dissolving the solid G5.NH2-(PEG-FA)-mPEG obtained in the step (2), adding a chloroauric acid solution, stirring, adding a NaBH4 solution, reacting, adding triethylamine and acetic anhydride after reacting, dialyzing a reaction product after reacting, and performing freeze drying. The method disclosed by the invention has the advantages of easiness, mild reaction conditions, easiness in operating and industrial implementation prospect; and the gold nanoparticle prepared with the method has the advantages of high water solubility, high stability, high water dispersion and the application prospect in the field of early detection of tumors.

The invention relates to a preparation method for chiral dendrimers/gold nanoparticles modified by mPEG (Methoxy Polyethylene Glycol), comprising the steps of: (1) modifying the chiral dendrimers by using mPEG-MAL (Maleimide) and dialyzing, freezing and drying to obtain solids; (2) dissolving the solids with methanol or water, and adding a chloroauric acid solution and then a NaBH4 solution after mixing, reacting at a room temperature; and then adding triethylamine and acetic anhydride, dialyzing a reaction product after the reaction is ended and freezing to obtain a final product by. The invention has the advantages of increasing molar ratio of the gold to the chiral dendrimers and reducing cost of materials, and at the same time enhancing stability of the gold nanoparticles and being successfully applied in the mice's in-vivo imaging; and the method has the advantages of simpleness, mild reaction conditions and easy operation, and has the prospect for the implementation of industrialization.

The invention relates to a polydopamine coated gold core/hollow silica shell nanometer material as well as preparation and application thereof. The nanometer material is prepared according to the following steps: coating gold nanoparticles and perfluorohexane (PFH) in the inner cavity of hollow mesoporous silica, and coating polydopamine on the surface of hollow mesoporous silica; stably reducingHAuCl4 with sodium citrate so as to obtain gold nanoparticles, taking ethyl orthosilicate as a silicon source, forming mesoporous solid silicon-coated gold nanoparticles in a mixed solution of ethanol, ultrapure water and ammonium hydroxide, and etching gold core/hollow silica shell nanoparticles with sodium carbonate; modifying amino on the surface of the nanoparticles, and coating perfluorohexane; finally, coating a polydopamine layer on the surface of the nanoparticles, thereby obtaining the product. The nano platform prepared by the method disclosed by the invention has good stability andexcellent biocompatibility, also has good US/CT/PA imaging and photothermal therapy effects, provides a novel method for development of the multimode imaging contrast agent and diagnosis and treatmentintegrated platform, and is wide in application prospects.

The invention relates to a preparation method of hollow mesoporous silica loaded by nanometer star-shaped gold particles. The method includes the steps of forming solid silicon wrapped by meso-porous silicon in an ethyl alcohol, ultrapure water and ammonia mixed solution with ethyl orthosilicate as a silicon source, etching the solid silicon through sodium carbonate to form hollow mesoporous silica, modifying the surface of the hollow mesoporous silica by sulfydryl and loading the surface of the hollow mesoporous silica with nanometer gold particles so that the hollow mesoporous silica can serve as seeds, making the seeds grow into the hollow mesoporous silica loaded by nanometer star-shaped gold particles in a chloroauric acid solution, and wrapping perfluorohexane and modifying polyethylene glycol with one end being -SH. The prepared nanometer particles have excellent biocompatibility and long in-vivo blood circulation time, have good US/CT/PA imaging and photo-thermal treatment effects and are wide in application prospects, and a new method is provided for development of multi-mode imaging contrast media and treatment integrated platforms.

The invention relates to a method for preparing a poly (amidoamine) dendritic polymer/carbon nanometer tube composite material for cancer cell targeting diagnosis. The method comprises that: a carbon nanometer tube CNT is subjected to acidizing treatment; fluorescein isothiocyanate FI and folic acid FA of the poly(amidoamine) dendritic polymer are modified; a CNT composite is obtained through an EDC chemical bonding method and a carboxyl reaction on the surface of a multi-walled carbon nanometer tube which is subjected to the acidizing treatment; terminal amino and acetyl oxide of the rest dendritic polymer on the surface of the composite are subjected to acetylation reaction to obtain a functional composite carbon nanometer tube with neutral surface charge. The functional carbon nanometer tube can be scattered in a solvent for a long time without agglomeration, and the functional multi-walled carbon nanometer tube has good biocompatibility, can be combined on the cancer cell in a targeting manner, and can be used for early-stage target diagnosis of the cancer cell; the method has the advantages of simple preparation method, mild reaction condition and easy operation, and the used polymer is an environment-friendly polymer material, and has industrial implementation prospect.

The invention relates to a preparation method for a ferroferric oxide nano particle targeted MRI contrast agent. The method comprises the following steps: 1, utilizing a hydrothermal method to synthesize PEI coated Fe3O4 nano particles Fe3O4-PE1; 2, conducting surface modification on fluorescein isothiocyanate FI by the Fe3O4-PE1 nano particles; 3, connecting hyaluronic acids of different molecular weights with surfaces of the nano particles to obtain the ferroferric oxide nano particle targeted MRI contrast agent. The method has the advantages of mild reaction conditions, simple operation process, and easiness in separating and purification. The prepared Fe3O4 magnetic nano particles have good colloid stability, biocompatibility and tumor targeting, and underlying application value in the field of in-vivo tumor targeted MRI diagnoses.

The invention relates to a preparation method for adriamycin-loaded polyethyleneimine-hyaluronic acid-modified hectorite-coated gold nanoparticles. The preparation method comprises the steps that a PLA-PEG-COOH solution is dropwise added into an LAP solution, stirring and activating are conducted, the mixture is dropwise added into a PEI solution to obtain LAP-PLA-PEG-PEI, chloroauric acid and NaBH4 are added, and LAP-PLA-PEG-PEI-(Au<0>)50 is obtained through a reaction; an activated HA solution is dropwise added into a LAP-PLA-PEG-PEI-(Au<0>)50 aqueous solution, stirring and reacting are conducted, DOX is added, reacting under stirring is conducted, and then the adriamycin-loaded polyethyleneimine-hyaluronic acid-modified hectorite-coated gold nanoparticles are obtained. The preparation method is low in cost, simple, mild in condition and easy to operate and has good application prospects. The nanomaterial-loaded DOX has a good drug slow release effect by serving as a nano-drug slow release system and has the significant targeting property and obvious inhibiting effect on tumor cells expressed by a CD44 receptor.

The invention relates to a preparation method of a hollow silicon-star gold core/shell nano material for wrapping adriamycin. The preparation method comprises the following steps of dissolving an HMSs (Hollow Mesoporous Silica Sphere), ultrasonically dispersing the dissolved HMSs, adding MPTMS (Mercaptopropyltrimethoxysilane) into the dispersed HMSs, introducing nitrogen into an obtained first mixture, refluxing the obtained first mixture in an oil bath, so as to obtain HMSs-SH (Hollow Mesoporous Silica Sphere-Sulfhydryl), afterwards, dissolving the HMSs-SH in water, adding an Au NPs (Aurum Nanoparticles) solution into an obtained second mixture, agitating the obtained second mixture, so as to obtain an HMSs/Au seed, dissolving the HMSs/Au seed in water, ultrasonically dispersing an obtained third mixture, adding a chloroauric acid solution into the dispersed third mixture, agitating an obtained fourth mixture, adding a silver nitrate solution and an ascorbic acid solution into the agitated fourth mixture, continuously agitating an obtained fifth mixture, so as to obtain an HMSs/Au NSs (Normal Saline Solution), dispersing the HMSs/Au NSs in water, adding an HS-PEG-RGD (Hassium-Polyethylene Glycol-Arginine-Glycine-Aspartic Acid) aqueous solution into an obtained sixth mixture, agitating an obtained seventh mixture, so as to obtain an HMSs/Au-PEG-RGD NSs, afterwards, dispersing the HMSs/Au-PEG-RGD NSs into ultrapure water, adding a DOX*HCL aqueous solution into an obtained eighth solution, agitating an obtained ninth mixture in a light-shielding manner, so that the hollow silicon-star gold core/shell nano material for wrapping the adriamycin is obtained. The nano material provided by the invention owns a specific targeting effect and excellent biocompatibility on a U87MG cell, has a synergistic enhancement effect on chemical therapy and photothermal therapy, and is wide in application prospect.

The invention relates to a folic-acid-modified laponite nanometer particle, preparation thereof and applications thereof. Laponite is modified by utilization of a silane coupling agent. The weight ratio of the laponite (LAP) to the silane coupling agent is 5:1-5:4. The weight percentage of the silane coupling agent and folic acid is 3.01-3.34%. The preparation includes: a step of, under stirring, adding dropwise an aqueous solution of the silane coupling agent into an aqueous solution of the laponite (LAP), reacting at 45-60 DEG C for 12-16 h and dialyzing to obtain aminated laponite LM-NH2; a step of dissolving the folic acid into a solvent, adding N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride EDC, and uniformly stirring to obtain a mixed solution; and a step of adding dropwise the aqueous solution of the aminated laponite LM-NH2 into the mixed solution, stirring for 2-3 d, and dialyzing. The laponite nanometer particle is used for loading medicines. The preparation has characteristics of simple method, mild reaction conditions and easy operation and has good prospect of industrial implementation.

The invention relates to a method for preparing a gold/silver nano particle with stable hydroxylated dendritic polymers, comprising the following steps: (1) using 50 ml of methyl alcohol to dissolve dendritic polymers, dropping epoxy reagent, diluting and dialyzing the solution, and mixing the left solution with water, freezing and drying; (2) dissolving the solid dried in the step (1) in methyl alcohol or water, and mixing HAuCl4 methanol solution or HAuCl4 water solution, and stirring the solution till the solution changes into deep red; or dissolving the solid dried in the step (1) in methyl alcohol or water, mixing AgNO3 dissolved in methyl alcohol/water mixed solution (v/v =1:1), and stirring till the solution changes into dark brown. The invention has the advantages of simple method, gentle reaction condition, easy operation and prospect of industrializing implementation.

The invention relates to an RGD peptide modified boron drug loading system, preparation thereof and an application of the system. An RGD peptide modified boron composite material serves as a drug carrier for loading drugs. Experimental conditions are easily controlled, operation is simple, and an obtained drug loading compound has good biocompatibility, can be slowly released in a sustained manner, has pH (potential of hydrogen) and NIR (near-infrared light) double sensitive drug releasing properties, is high in release rate in low-pH value and near-infrared light irradiation environments andsuitable for microenvironments of tumor tissues, can be used for achieving synergistic effects of low-temperature photothermal therapy and chemotherapy and has application prospects in preparation oftumor targeted, imaging and synergistic therapy drugs.

The invention relates to lithium amide soapstone nano-particles modified by polyethylene glycol-folic acid as well as preparation and application of the lithium amide soapstone nano particles. Lithium amide soapstone is lithium soapstone modified by (3-aminopropyl) ethoxydimethyl-silane APMES; the mol ratio of polyethylene glycol to folic acid is (1:0.4)-(1:0.8); and the mass percentage of polyethylene glycol-folic acid is 45-55%. The preparation disclosed by the invention comprises the following steps of dissolving folic acid in a solvent, adding 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride EDC, stirring to obtain mixed solution, adding the mixed solution into polyethylene glycol solution drop by drop, stirring in 2-3 d, dialyzing, freezing, drying, and obtaining polyethylene glycol-folic acid PEG-FA; and adding EDC into PEG-FA solution, stirring for 2-4 h, adding into aqueous solution of the lithium amide soapstone nano particles drop by drop, magnetically stirring in 2-3 d and dialyzing, thereby obtaining the lithium amide soapstone nano particles modified by polyethylene glycol-folic acid. The lithium amide soapstone nano particles modified by polyethylene glycol-folic acid are applied to drug load, have the advantages of simple preparation method, moderate condition and wide application range, and have good market prospect.

The invention relates to a hyaluronic acid-modified amide hectorite nanoparticle and a preparation and an application of the hyaluronic acid modified hectorite amide nanoparticle. The hyaluronic acid modified hectorite amide nanoparticle is (3-amino propyl) ethoxydimethylsilane modified hectorite; and the mass fraction of the hyaluronic acid in the nanoparticle is 17.17%-19.09%. The preparation method comprises the following steps: modifying a hectorite surface with (3-amino propyl) ethoxydimethylsilane on to obtain hectorite amide; and through covalent action of amino and carboxyl, grafting the hectorite amide surface with hyaluronic acid, thus obtaining the hyaluronic acid modified hectorite amide nanoparticle. The stability and the biocompatibility of the nanoparticle are improved; meanwhile, the hyaluronic acid-modified hectorite amide nanoparticle has specific targeting action on cancer cells with high expression for a CD44 receptor, and can be applied to targeted delivery of anti-cancer drugs; and the hyaluronic acid-modified hectorite amide nanoparticle is simple in preparation method, mild in reaction condition and easy to operate, and has an industrial implementation prospect.

The invention relates to a preparation method of tree-like macromolecular and stable ultra-small ferroferric oxide/gold nano-flowers. The preparation method comprises the following steps: mixing FeCl3, sodium citrate and anhydrous sodium acetate and reacting to obtain ultra-small Fe3O4NPs; carrying out activation treatment to obtain activated ultra-small Fe3O4NPs; enabling a G5 solution to react with a chloroauric acid solution to obtain G5-Au NPs; enabling the G5-Au NPs to react with the ultra-small Fe3O4NPs, so as to obtain G5-Fe3O4-Au NPs; enabling the chloroauric acid solution, an AgNO3 solution and an ascorbic acid AA solution to react, so as to obtain a G5-Fe3O4-AuNFs solution; carrying out acetylation treatment to obtain a product. Nanoparticles prepared by the preparation method have excellent biocompatibility and a good MR/CT/PA imaging function; the nanoparticles can be used for photothermal therapy of tumors; a photothermal therapy effect is improved through radiotherapy andthe nanoparticles can be used for a potential tumor multi-modal imaging contrast agent and combined therapy and have a wide application prospect.

The invention relates to a preparation method of laponite nanoparticles ICG (Indocyanine Green)/LAP (Laponite) supportng the ICG. The preparation method comprises the following steps: dissolving the ICG into ultrapure water and carrying out light-avoiding stirring under the condition of normal temperature to obtain an aqueous solution of the ICG; dropwise adding the aqueous solution of the ICG into an aqueous solution of the LAP, and uniformly mixing; then carrying out room temperature light-avoiding stirring reaction under the condition of a buffer solution, and centrifugally purifying to obtain a finished product. According to the ICG/LAP nanoparticles, stability and light and heat stability of the ICG in the aqueous solution are improved, the photothermal conversion efficiency of the ICG is improved, and a new idea is provided for subsequent application of the ICG in the biological field.

The invention relates to a preparation method for a PEI-coated bimodal contrast agent, i.e., a ferriferrous oxide-gadolinium hydroxide magnetic nanoparticle. The preparation method comprises the following steps: (1) dissolving an Fe source in ultrapure water, then adding NH3.H2O, carrying out stirring in the air to realize oxidation, then adding an aqueous solution of polyethylene imine (PEI) and an aqueous solution of Gd(NO3)3 and carrying out natural cooling to room temperature after completion of a reaction so as to obtain Fe3O4-Gd(OH)3-PEI; and (2) subjecting Fe3O4-Gd(OH)3-PEI and methoxypolyethylene glycol carboxylic acid to pegylation. The preparation method provided by the invention has the advantages of simple process, mild reaction conditions and easy operation and separation; the prepared ferriferrous oxide-gadolinium hydroxide magnetic nanoparticle (Fe3O4-Gd(OH)3-PEI-PEG) has good biocompatibility, good relaxation effects at T1 and T2 and a latent application value in the field of MRI imaging diagnosis.

The invention relates to a polyethylene glycol-lactobionic acid modified aminated hectorite nano particle as well as a preparation method and application thereof. The preparation method of the polyethylene glycol-lactobionic acid modified aminated hectorite nano particle comprises the following steps: firstly, carrying out reaction on lactobionic acid with polyethylene glycol, wherein one end of the polyethylene glycol is an amino group, and the other end of the polyethylene glycol is a carboxyl, so that pegylated lactobionic acid is obtained; secondly, modifying hectorite with (3-aminopropyl) dimethyl ethoxy silane, so that a certain amount of amino groups are formed on the surface of the hectorite; thirdly, modifying nano clay hectorite with the pegylated lactobionic acid, so that a lactobionic acid modified functional hectorite nano composite material is obtained; the lactobionic acid modified functional hectorite nano composite material can be used for preparing a nano medicine carrying system with a lactobionic acid targeting function. The polyethylene glycol-lactobionic acid modified aminated hectorite nano particle has the advantages that stability and biological compatibility of a nano particle are improved, and a specific targeting effect on liver cancer cells highly expressed by an asialoglycoprotein receptor is realized, so that a synthesized nano medicine carrying composite material can be applied to targeted delivery of an anti-cancer drug. The preparation method of the polyethylene glycol-lactobionic acid modified aminated hectorite nano particle is simple, mild in reaction conditions and easy to operate and has an industrialization implementation prospect.

The invention relates to a manganese dioxide and doxorubicin supported nanometer hydrogel and preparation and application thereof. The manganese dioxide and doxorubicin supported nanometr hydrogel isprepared by supporting manganese dioxide and drugs to poly-N-vinyl-epsilon-caprolactam nano hydrogel. The preparation method is simple, the product is easy to purify, and the cost is low. The nanometer hydrogel prepared by using the method is low in particle diameter, is uniformly distributed, and is high in water solubility, colloidal stability and biocompatibility. The nanometer hydrogel prepared by using the method can be used as a magnetic resonance imaging contrast agent, in the combination of an ultrasound-targeted microbubble destruction technology, proliferation of cancer cells can besignificantly inhibited, and the manganese dioxide and doxorubicin supported nanometer hydrogel has a potential application value in the field of tumor diagnosis and treatment.

The invention relates to a preparation method of a pH-responsive core-shell structure dendrimer drug carrier. The fifth generation dentrimer modified by beta-cyclodextrin is taken as a core, the thirdgeneration dentrimer modified by benzimidazole-2-acetic acid is taken as a shell, and a pH-responsive core-shell super structure dendrimer is constructed by the self-assembly function of a supramolecule of host and guest molecules. When the prepared pH-responsive core-shell structure dendrimer is used as an anticancer drug carrier, the advantages of low toxicity, safety, high loading rate, intelligent release in tumor microenvironment and the like are achieved, and potential application prospects are achieved in aspects of chemotherapy and the like.

The invention relates to a drug-loaded fiber ring and a preparation method and application thereof. The method comprises preparing a DOX/PLGA drug-loaded nanofiber membrane by electrospinning, and homogenizing to obtain the DOX/PLGA drug-loaded fiber ring. The surface is subjected to hyperbranched PEI chelating Gd(III) for MR imaging, and a modified DNA aptamer acts as a targeting molecule to be tightly bound to cancer cells, which can effectively inhibit tumor metastasis and invasion. The preparation method has the advantages of simple preparation process and good material biosafety. The ringmaterial is more likely to anchor the cancer cells and reduce the shedding of tumor cells. An internally loaded drug can effectively act on the tumor tissues for a long time, and effectively inhibitthe metastasis while killing the cancer cells. The fiber ring has broad application prospects in the field of cancer treatment.

The invention relates to a preparation method of a polylactic acid hydroxyacetic acid based composite nano fiber membrane for high-efficiency adsorption of mercury ions, wherein the preparation method comprises the steps: modifying the surface of a fifth-generation polyamide-amine dendritic macromolecule G5.NH2 by PEGylated thymine PEG-T, to obtain G5-PEG-T, and then mixing the G5-PEG-T with polylactic acid hydroxyacetic acid PLGA, to obtain a PLGA/G5-PEG-T composite nano fiber membrane by an electrostatic spinning method. The used raw materials are relatively low in price; and the prepared polylactic acid hydroxyacetic acid based composite nano fiber membrane has excellent specificity and high-efficiency Hg<2+> adsorption effect, has simple preparation process, and can be used for treatment of Hg<2+>-containing wastewater in chlorine-alkali manufacturing, papermaking, oil refining, paints, pharmacy, battery manufacturing and other industrial production.

The invention relates to a surface modification method of nanometer hydroxyapatite mediated by APTS, which includes (1) mixing the nanometer hydroxyapatite n-HA with ethanol and dispersing the mixture through ultrasonic wave to obtain n-HA ethanol suspending liquid; (2) adding the APTS into the n-HA ethanol suspending liquid in dropwise mode and conducting centrifuging, washing, separating and precipitating on the suspending liquid after reaction is finished under room temperature condition to obtain n-HA-APTS nanometer particles; or further conducting acetylation or carboxylation on the n-HA-APTS nanometer particles. The surface modification method is mild in condition and simple in process. Products are easy to separate, and the used modification agent is environment-friendly silane materials. The obtained n-HA-APTS, n-HA-APTS.AC and n-HA-APTS.SAH nanometer particles have different active groups on the surfaces, have good biocompatibility and blood cell stability and are wide in application prospect.