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Functional nano material drug delivery system for identifying, capturing and restraining circulating tumor cells

A drug-loading system and functional nanotechnology, which is applied in the fields of antineoplastic drugs, drug combinations, and pharmaceutical formulations, etc.

Inactive Publication Date: 2015-02-18
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current application of nanotechnology in cancer also mainly focuses on the combination of postoperatively transferred chemical drugs and monoantibody-coated nanomaterials, but this method cannot effectively intervene due to its toxicity and non-specificity. The process of cancer metastasis caused by circulating tumor cells

Method used

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  • Functional nano material drug delivery system for identifying, capturing and restraining circulating tumor cells
  • Functional nano material drug delivery system for identifying, capturing and restraining circulating tumor cells
  • Functional nano material drug delivery system for identifying, capturing and restraining circulating tumor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Preparation of polyamide-amine dendrimer complex coated with double antibody

[0046] Polyamidoamine dendrimer complexes coated with or without fluorescently labeled antibodies can be obtained by covalently linking antibodies to polyamidoamine dendrimers for specific recognition of CTCs in vitro , capture and activity regulation, the specific preparation process is as follows figure 1 shown.

[0047] (1) Complete carboxylation modification of polyamide-amine dendrimers:

[0048] Take 60 mg of the 6th generation polyamide-amine dendrimers (G6 PAMAM dendrimers) dissolved in methanol containing 60 mg of amino-terminated dendrimers. After rotary evaporating the methanol solution, dissolve it completely with 2 ml of DMSO solution, and then add 246 mg of succinyl Anhydrides were reacted under strong magnetic stirring at room temperature for 24 h, and then the reaction product was dialyzed in 500 ml×3 ultrapure water with a cellulose dialysis membrane with a mole...

Embodiment 2

[0059] Example 2: Characterization of polyamide-amine dendrimer complexes coated with double antibodies

[0060] (1) Take a certain amount of newly prepared CC G6, G6-5aEpCAM-5aSlex substances, and use 700 μl of D 2 After the O is fully dissolved, place it in an NMR tube, and then use a 400 MHz superconducting NMR instrument to perform 1 H NMR analysis. (2) Take a certain amount of newly prepared CC G6 and G6-5aEpCAM-5aSlex substances, fully dissolve them in PBS (pH 7.4), treat them with ultrasonic waves for 5 min, filter them through a 0.22 μm disposable water-soluble filter, and put them in DLS / Zeta potential instrument was used to measure the respective water-soluble particle size and potential distribution. (3) Take a certain amount of newly prepared CC G6, G6-5aEpCAM-5aSlex substances, mix them with an appropriate amount of KBr powder, press them into tablets, and test their FTIR spectra in an infrared analyzer.

[0061] (4) Take a certain amount of newly prepared CC G...

Embodiment 3

[0066] Example 3: In vitro recognition and capture analysis of double-antibody-coated polyamide-amine dendrimer complexes on CTCs model (HT29 cells) (1) HT29 cells in logarithmic growth phase were treated with 10 5 Inoculated on a 35 mm laser confocal special dish at a density of 35 mm per ml. After the cells adhered to the wall, they were suspended and blocked with PBS containing 1% BSA for 30 min. After repeated washing, they were mixed with fluorescently labeled Double antibody complex (PE-5aEpCAM-G6-3aSlex-FITC) at 37 °C, 5% CO 2 co-cultivated in an incubator for 1 h. Finally, unbound complexes or antibodies were washed away, fixed with a fixative, and stained in PBS solution containing nuclear staining agent DAPI for 15 min in the dark. After staining, wash repeatedly, and finally cover with serum-free and phenol-red-free medium and perform laser confocal microscope DAPI λ ex 405 nm, lambda em 425-475 nm, FITC lambda ex 488 nm, lambda em 500-535 nm, PE λ ex 550 ...

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Abstract

The invention belongs to the field of application of a nano material coating technology in identifying, capturing and activity regulating of circulating tumor cells. For pre-warning and preventing of cancer metastasis, particularly, the invention relates to a functional nano material drug delivery system for identifying, capturing and restraining circulating tumor cells. The functional nano material drug delivery system consists of a central nano material carrier, a surface targeted antibody or an aptamer, and a drug for resisting cancers or preventing the cancer metastasis. The functional nano material drug delivery system disclosed by the invention can be used for in-vitro simulation or specific identification and capturing research of trace circulating tumor cells of a blood sample of a clinical patient, and also can be used for regulating the activities of the captured circulating tumor cells, so that the application prospect in pre-warning and preventing of the cancer metastasis is expanded.

Description

technical field [0001] The invention belongs to the application field of nanomaterial coating technology in the recognition, capture and activity regulation of circulating tumor cells, especially for the early warning and prevention of cancer metastasis, and in particular relates to a functional nanometer that specifically recognizes, captures and inhibits circulating tumor cells. Material drug delivery system. Background technique [0002] Over the past 50 years, the mortality rate of many major diseases (such as heart disease) has decreased by >60%, but the cancer mortality rate has only decreased by about 5%, and most of them died of tumor re-metastasis after surgery. Traditional "anticancer drugs" mainly target those malignantly proliferating tumor cells rather than dormant circulating tumor cells, so not only cannot selectively kill circulating tumor cells, but also defeat the body's immune system, making "disease - The balance between the human body's own disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K47/42A61K47/34A61K47/04A61K9/14A61P35/00A61K31/56A61K31/567A61K31/401A61K31/122
Inventor 贾力谢静静陈红宁
Owner FUZHOU UNIV
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