10 results about "Activity regulation" patented technology

This invention relates to the fields of microbial genetic engineering and fermentation biomanufacturing technology, specifically disclosing a recombinant Corynebacterium glutamicum with high 5-aminolevulinic acid (ALA) production and its applications. Addressing the problems of existing strains such as crude enzyme activity regulation, inhibited cell growth, the need for external inhibitors, and low yield, this invention uses Corynebacterium glutamicum as a host and removes the gdhA gene without scarring to reduce precursor competitive metabolism. Site-directed mutations of the endogenous hemB gene (S288A, D128E, R231K) are performed to moderately reduce dehydratase activity. A high-expression plasmid pXMJ19-hemA containing the hemA gene from Rhodopsinus capsulatum is constructed and introduced into the modified strain to obtain a recombinant strain with optimized triple metabolism. This strain reduces product degradation without the need for external dehydratase inhibitors, does not affect cell growth, and achieves a yield up to 4.30 times higher than the control after 42 hours of fermentation, with ALA accumulation exceeding 8.9 g / L. The strain exhibits stable characteristics, the process is simple, and it has good industrial application value.

The invention provides a liquid organic fertilizer preparation method combining biological compost purification and activity regulation, and relates to the technical field of agricultural resource recycling and organic fertilizer preparation, and the method comprises the following steps: carrying out hydrothermal extraction treatment on a biological compost raw material to form a compost extraction liquid, carrying out solid-liquid separation treatment on the compost extraction liquid to form a primary filtrate, and carrying out solid-liquid separation treatment on the primary filtrate to form a secondary filtrate; the hydrothermal extraction treatment comprises temperature control, stirring homogenization and dissolution promotion. And carrying out enzymatic composite reaction treatment on the purified liquid to form an activated liquid, wherein the enzymatic composite reaction treatment comprises adding a specific enzyme system to carry out a catalytic reaction. According to the liquid organic fertilizer preparation method combining biological compost purification and activity regulation and control, efficient preparation of the liquid organic fertilizer is achieved by combining biological compost purification and activity regulation and control technologies. Effective components are extracted from biological compost raw materials through a hydrothermal extraction technology, multi-stage synergistic purification is carried out, impurities are removed, and it is ensured that the finally prepared liquid fertilizer has high purity and biological activity.

The application discloses an exonuclease fusing an NTPase domain and application thereof, and belongs to the technical field of bioengineering. The exonuclease is a Ppl protein, which comprises a PHP domain at the N terminal and an NTPase domain at the C terminal, wherein the PHP domain has 3'-5' ssDNA exonuclease activity. The NTPase domain of the application regulates the exonuclease activity of the PHP domain through conformational change. Under the condition of high NTP concentration, the NTPase domain inhibits the activity of the PHP domain; when the NTP concentration decreases, the inhibition is released, and the exonuclease activity is significantly activated. The activated PHP domain shows specific cleavage activity to DNA substrates with 3'-hydroxyl overhang. The unique 'NTP concentration sensing and enzyme activity regulation' characteristics make it a core module for developing biological sensors, gene circuits and controllable nucleic acid tools.

The invention discloses a fusion polypeptide, an anti-KIR2DL1 monoclonal antibody and an anti-aging composition containing the fusion polypeptide and the anti-KIR2DL1 monoclonal antibody. Wherein the amino acid sequence of the fusion polypeptide is SEQ ID NO: 1. A heavy chain variable region of the anti-KIR2DL1 monoclonal antibody is SEQ ID NO: 2, and a light chain variable region of the anti-KIR2DL1 monoclonal antibody is SEQ ID NO: 3. The composition composed of the two components regulates NK cells bidirectionally by enhancing an NKG2D activation pathway and blocking a KIR2DL1 inhibition pathway, so that the proportion of CD107a positive NK cells reaches 68.9%, the secretion amount of granzyme B reaches 465pg / mL, the survival rate of senescent cells is reduced to 39%, and the synergistic effect exceeds 89%. The composition can be prepared into intravenous injection drugs, oral health care products or external cosmetics, is safe and remarkable in synergistic effect, and solves the problems of insufficient curative effect of a single activator and poor specificity of a single inhibitor in the prior art.

The invention discloses a starting method of a diesel hydrocracking unit, which comprises the following steps: after a catalyst is vulcanized, introducing starting-assisting oil into a liquid-phase material, heating to a reaction temperature to complete activity regulation and control at the initial stage of starting, and then switching to introduce a fresh raw material; the start-up assisting oil is a mixture of light naphtha and heavy naphtha. According to the start-up method, the property of a material contacted with the diesel hydrocracking catalyst after vulcanization is completed is controlled, so that the activity of the catalyst is regulated and controlled, the activity of the catalyst at the initial stage is kept at a high level and is stable and controllable, and the operation cycle of a device is further prolonged. And the risk of temperature runaway of the reaction temperature in the raw material switching link can be greatly reduced while the initial activity of the diesel oil hydrocracking catalyst is retained to the greatest extent.

The utility model discloses a cell activation dressing with an active cell cabin and a nutrient communicating channel, which relates to the technical field of focus tissue repair, and comprises a patch, an active cell cabin and a nutrient communicating channel, the cell treatment nursing carrying table is arranged on the patch and acts on the focus tissue; the bionic structure is arranged in the cell treatment care platform deck and is used for guiding and stimulating neogenesis cell proliferation; the activity adjusting input device is communicated with the cell treatment nursing platform through a conduit and is used for providing nutrients for rapid tissue repair and healing in the basic cell therapy; the cell treatment and care platform deck is used as a carrier of a bionic structure, and the activity regulation input device and the metabolic balance output device are used for providing an effective migration and proliferation space for cells, promoting culture solution drainage supply and metabolism-generated waste liquid drainage and removal, effectively guaranteeing activity regulation and metabolic balance, and improving the treatment effect of the cells. And improvement of the chronic / large-area tissue repair efficiency is facilitated.

This invention relates to the field of food processing technology, providing a low-temperature preservation and synergistic pretreatment method adaptable to various protein sources. The method includes: subjecting the protein source raw material to hypertonic pretreatment, causing tissue fluid to migrate and precipitate off-odor substances; performing synergistic deodorization and activity regulation treatment at a temperature not exceeding 70°C; subsequently performing low-temperature enzyme inactivation and structural stabilization treatment, followed by sterilization using a low-temperature or heatless method; and finally processing it into a standardized intermediate suitable for food systems. This technical solution achieves effective removal of off-odors from protein sources at relatively low temperatures, while minimizing damage to protein structure and active ingredients, thus improving the nutritional retention and quality stability of the product. The method is applicable to animal-derived, plant-derived, and microbial-derived proteins, and can be widely used in various food systems, demonstrating good versatility and industrial application value.

A system for controlling a vehicle, including one or more communication modules and one or more processors operably coupled to the communication modules. The one or more processors are configured to individually or collectively: receive a geo-fence identifier associated with geo-fence information, where the geo-fence identifier uniquely identifies the geo-fence from other geo-fences; obtain one or more activity regulations for the vehicle based on the geo-fence identifier; and control operation of the vehicle according to the one or more activity regulations.

A method for preparing and applying a double-sided dura mater sealant capable of regulating fibroblast activity, belonging to the field of medical biomaterials, is disclosed. The double-sided dura mater sealant consists of an A-side facing the dura mater layer and a B-side facing the muscle layer, wherein the A-side facing the dura mater layer comprises a central portion and a peripheral portion. The method includes the following steps: preparing the A-side facing the dura mater layer; preparing the B-side facing the muscle layer; and assembling the double-sided dura mater sealant. This invention possesses the ability to bidirectionally regulate fibroblast activity, both enhancing dura mater fibroblast activity and inhibiting extradura mater fibroblast activity, thus resolving the contradiction in fibroblast activity regulation during dura mater injury repair and extradura mater fibrosis prevention. Simultaneously, it provides reliable cerebrospinal fluid leakage sealing performance, solving the problem of simultaneously achieving dura mater injury repair and extradura mater fibrosis prevention.

The utility model discloses centrifugal equipment for cleaning agents, which relates to the technical field of chemical mechanical equipment and comprises a shell, a driving device and a separating component, a first cavity and a second cavity which are vertically through are arranged in the shell, and the first cavity is connected with the second cavity through a communicating pipe; a feeding hole is formed in the top of the first chamber, a slag discharge hole is formed in the bottom of the first chamber, and a liquid outlet is formed in the bottom of the second chamber. According to the centrifugal equipment for the cleaning agent, provided by the utility model, the efficient separation and purification of the cleaning agent are realized through the synergistic effect of the multi-stage filter screen, the dynamic stirring device, the annular heating plate, the filter element and the intelligent monitoring system. And the design and arrangement of each part are carried out by improving the separation efficiency, optimizing the activity regulation and control capability of the cleaning agent and enhancing the intelligent operation of the equipment, so that the equipment can meet the requirements of various complex working conditions in practical application.