19 results about "Activity regulation" patented technology

This invention relates to the fields of microbial genetic engineering and fermentation biomanufacturing technology, specifically disclosing a recombinant Corynebacterium glutamicum with high 5-aminolevulinic acid (ALA) production and its applications. Addressing the problems of existing strains such as crude enzyme activity regulation, inhibited cell growth, the need for external inhibitors, and low yield, this invention uses Corynebacterium glutamicum as a host and removes the gdhA gene without scarring to reduce precursor competitive metabolism. Site-directed mutations of the endogenous hemB gene (S288A, D128E, R231K) are performed to moderately reduce dehydratase activity. A high-expression plasmid pXMJ19-hemA containing the hemA gene from Rhodopsinus capsulatum is constructed and introduced into the modified strain to obtain a recombinant strain with optimized triple metabolism. This strain reduces product degradation without the need for external dehydratase inhibitors, does not affect cell growth, and achieves a yield up to 4.30 times higher than the control after 42 hours of fermentation, with ALA accumulation exceeding 8.9 g / L. The strain exhibits stable characteristics, the process is simple, and it has good industrial application value.

A system, vehicle, and method for controlling a vehicle using geo-fence-based activity regulations that change over time. The system includes processor(s) configured to receive a geo-fence identifier that uniquely identifies a geo-fence, obtain one or more activity regulations for the vehicle based on the geo-fence identifier, and control operation of the vehicle according to a set of activity regulations applicable based on a current time. The activity regulations change over time such that a first set of activity regulations applicable at a first time differs from a second set applicable at a second time. At least one of boundaries or restrictions associated with the geo-fence may change over time. The geo-fence may comprise a dynamic indicator that changes over time to reflect changes in the activity regulations. The activity regulations may change periodically, in accordance with a pre-set schedule, or in response to a detected event or condition.

The invention relates to the technical field of building materials, and discloses a regenerated micro powder preparation and activity regulation method, which comprises the following steps: raw material pretreatment, crushing and screening of building wastes, impurity adsorption treatment, removal of impurities such as clay in the raw materials through physical adsorption, and mechanical ball milling activation. And carrying out mechanical ball milling, surface modification treatment, thermal activation treatment and intelligent composite excitation on the pretreated recycled aggregate. Through an excitation mode of mixing sodium silicate and regenerated micro powder by a dry method, cracks on the surface of the micro powder are embedded by mechanical force in the ball milling process, so that the penetration is more uniform than that of a solution wet method, and the activity of the regenerated micro powder can be better excited. In addition, the activity of the regenerated micro-powder can be remarkably improved through innovation of the process sequence, the activity index of the regenerated micro-powder which is excited after ball milling is 65.1%, the activity index of the regenerated micro-powder which is subjected to ball milling, exciting agent adding and ball milling is 82.4%, and finally, solution evaporation energy consumption can be omitted, and the cost is reduced.

The invention provides a liquid organic fertilizer preparation method combining biological compost purification and activity regulation, and relates to the technical field of agricultural resource recycling and organic fertilizer preparation, and the method comprises the following steps: carrying out hydrothermal extraction treatment on a biological compost raw material to form a compost extraction liquid, carrying out solid-liquid separation treatment on the compost extraction liquid to form a primary filtrate, and carrying out solid-liquid separation treatment on the primary filtrate to form a secondary filtrate; the hydrothermal extraction treatment comprises temperature control, stirring homogenization and dissolution promotion. And carrying out enzymatic composite reaction treatment on the purified liquid to form an activated liquid, wherein the enzymatic composite reaction treatment comprises adding a specific enzyme system to carry out a catalytic reaction. According to the liquid organic fertilizer preparation method combining biological compost purification and activity regulation and control, efficient preparation of the liquid organic fertilizer is achieved by combining biological compost purification and activity regulation and control technologies. Effective components are extracted from biological compost raw materials through a hydrothermal extraction technology, multi-stage synergistic purification is carried out, impurities are removed, and it is ensured that the finally prepared liquid fertilizer has high purity and biological activity.

The invention provides a molecular chiral self-assembly and chiral optical activity regulation and control method. The method comprises the following steps: dispersing a porphyrin raw material in an aqueous solution containing a first surfactant to prepare a porphyrin dispersion liquid; dispersing carbon nanotubes in an aqueous solution containing a second surfactant to prepare a carbon nanotube dispersion liquid; and mixing the porphyrin dispersion liquid and the carbon nanotube dispersion liquid, and standing to enable porphyrin molecules to spontaneously form chiral self-assembly on the surface of the carbon nanotube. By adjusting the mass ratio of the porphyrin to the carbon nanotubes, continuous regulation and control of the optical activity of the assembly can be realized. According to the method, chiral self-assembly of achiral molecules can be realized without a chiral substance, a carrier or a catalyst. The optical activity of porphyrin molecules can be accurately regulated and controlled by adjusting the proportion, the operation is simple and efficient, and an important foundation is laid for chiral optical activity system preparation, chiral recognition, sensing, mirror image body selective catalysis and other applications.

The present invention relates to a mutant LC3B allosteric switch, wherein three specific residues have been mutated to either reduce or enhance receptor binding, compared to the wild- type protein. In particular, the present invention provides a method for generating conformational ensemble of lipidated protein on the membrane and introducing specific mutations within the protein activation switch that is indirectly altering protein binding pocket without disrupting its functional domains. The identification of an activation site opens avenues for the development of small molecules or peptides to enhance or regulate protein activity, providing a new dimension for therapeutic interventions. Therefore, by linking the structural insights of lipidated forms to functional outcomes, the invention advances knowledge on LC3 activity regulation, which could further be utilized to modulate the autophagy pathways.

The application discloses an exonuclease fusing an NTPase domain and application thereof, and belongs to the technical field of bioengineering. The exonuclease is a Ppl protein, which comprises a PHP domain at the N terminal and an NTPase domain at the C terminal, wherein the PHP domain has 3'-5' ssDNA exonuclease activity. The NTPase domain of the application regulates the exonuclease activity of the PHP domain through conformational change. Under the condition of high NTP concentration, the NTPase domain inhibits the activity of the PHP domain; when the NTP concentration decreases, the inhibition is released, and the exonuclease activity is significantly activated. The activated PHP domain shows specific cleavage activity to DNA substrates with 3'-hydroxyl overhang. The unique 'NTP concentration sensing and enzyme activity regulation' characteristics make it a core module for developing biological sensors, gene circuits and controllable nucleic acid tools.

The invention discloses a fusion polypeptide, an anti-KIR2DL1 monoclonal antibody and an anti-aging composition containing the fusion polypeptide and the anti-KIR2DL1 monoclonal antibody. Wherein the amino acid sequence of the fusion polypeptide is SEQ ID NO: 1. A heavy chain variable region of the anti-KIR2DL1 monoclonal antibody is SEQ ID NO: 2, and a light chain variable region of the anti-KIR2DL1 monoclonal antibody is SEQ ID NO: 3. The composition composed of the two components regulates NK cells bidirectionally by enhancing an NKG2D activation pathway and blocking a KIR2DL1 inhibition pathway, so that the proportion of CD107a positive NK cells reaches 68.9%, the secretion amount of granzyme B reaches 465pg / mL, the survival rate of senescent cells is reduced to 39%, and the synergistic effect exceeds 89%. The composition can be prepared into intravenous injection drugs, oral health care products or external cosmetics, is safe and remarkable in synergistic effect, and solves the problems of insufficient curative effect of a single activator and poor specificity of a single inhibitor in the prior art.

The invention provides an enzyme activity regulation and control combined stepped temperature control shiitake mushroom drying method which comprises the following specific steps: shiitake mushrooms are placed in a composite enzymatic hydrolysate to obtain a first treated substance, and the composite enzymatic hydrolysate comprises coenzyme; placing the first treated object in an alkali metal cation mixed solution for ultrasonic treatment to obtain a second treated object; and carrying out stepped heating and drying on the third treated material to obtain the dried shiitake mushroom rich in sulfur flavor. By combining enzymolysis, coenzyme addition, ultrasound and programmed temperature control drying technologies, the production and retention conditions of flavor substances in the shiitake mushroom drying process are optimized, the sulfur flavor of shiitake mushrooms is remarkably improved, meanwhile, the drying efficiency is improved, and the nutritional value of the product is kept.

The invention discloses a starting method of a diesel hydrocracking unit, which comprises the following steps: after a catalyst is vulcanized, introducing starting-assisting oil into a liquid-phase material, heating to a reaction temperature to complete activity regulation and control at the initial stage of starting, and then switching to introduce a fresh raw material; the start-up assisting oil is a mixture of light naphtha and heavy naphtha. According to the start-up method, the property of a material contacted with the diesel hydrocracking catalyst after vulcanization is completed is controlled, so that the activity of the catalyst is regulated and controlled, the activity of the catalyst at the initial stage is kept at a high level and is stable and controllable, and the operation cycle of a device is further prolonged. And the risk of temperature runaway of the reaction temperature in the raw material switching link can be greatly reduced while the initial activity of the diesel oil hydrocracking catalyst is retained to the greatest extent.

The utility model discloses a cell activation dressing with an active cell cabin and a nutrient communicating channel, which relates to the technical field of focus tissue repair, and comprises a patch, an active cell cabin and a nutrient communicating channel, the cell treatment nursing carrying table is arranged on the patch and acts on the focus tissue; the bionic structure is arranged in the cell treatment care platform deck and is used for guiding and stimulating neogenesis cell proliferation; the activity adjusting input device is communicated with the cell treatment nursing platform through a conduit and is used for providing nutrients for rapid tissue repair and healing in the basic cell therapy; the cell treatment and care platform deck is used as a carrier of a bionic structure, and the activity regulation input device and the metabolic balance output device are used for providing an effective migration and proliferation space for cells, promoting culture solution drainage supply and metabolism-generated waste liquid drainage and removal, effectively guaranteeing activity regulation and metabolic balance, and improving the treatment effect of the cells. And improvement of the chronic / large-area tissue repair efficiency is facilitated.

The invention discloses application of an oat AsSAD8 gene in seed activity regulation and control, and belongs to the technical field of seed biology. The nucleotide sequence of the oat AsSAD8 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the oat AsSAD8 gene is as shown in SEQ ID NO. 2. The over-expression of the AsSAD8 gene significantly reduces the germination rate of the aged seeds of arabidopsis thaliana and rice, indicates the potential negative effect of the gene in the seed activity, and reveals that the expression of the AsSAD8 gene is inhibited, the seed aging is delayed, the seed life is prolonged, and the seed preservation quality is improved.

This invention relates to the field of food processing technology, providing a low-temperature preservation and synergistic pretreatment method adaptable to various protein sources. The method includes: subjecting the protein source raw material to hypertonic pretreatment, causing tissue fluid to migrate and precipitate off-odor substances; performing synergistic deodorization and activity regulation treatment at a temperature not exceeding 70°C; subsequently performing low-temperature enzyme inactivation and structural stabilization treatment, followed by sterilization using a low-temperature or heatless method; and finally processing it into a standardized intermediate suitable for food systems. This technical solution achieves effective removal of off-odors from protein sources at relatively low temperatures, while minimizing damage to protein structure and active ingredients, thus improving the nutritional retention and quality stability of the product. The method is applicable to animal-derived, plant-derived, and microbial-derived proteins, and can be widely used in various food systems, demonstrating good versatility and industrial application value.

The present invention relates to the application of PRSS56 protein expression and enzyme activity regulation in myopia treatment. The present invention uses a method for whole-genome linkage analysis, haplotype analysis, whole-genome sequencing combined with Sanger sequencing verification to detect and verify pathogenic sites for two large families with hereditary high myopia and 236 cases of early-onset high myopia in children. Combined with the family's genetic pattern, mutant gene frequency and animal model data, it is confirmed that mutations in the upstream promoter region of the PRSS56 gene translation start c.-187G>T, c.-187G>C, c.-297C>T, c.-378G>A, c.-382C>T and c.-421G>A can lead to the occurrence of hereditary high myopia, and the mutation is an autosomal heterozygous mutation. Based on this, a hereditary high myopia screening kit and a high myopia animal model for detecting the above mutation sites are provided. The application of the animal model and the kit of the present invention can provide a scientific basis for the early diagnosis, differential diagnosis and drug treatment of hereditary high myopia, which is conducive to early prevention and treatment and improves the quality of life of patients.

The invention relates to an application of camelliin B in inhibiting the activity of protein disulfide bond isomerase. The invention discloses a novel application of camellia glycoside B. On the basis of finding that a natural flavonoid compound camellia glycoside B has the activity of inhibiting protein disulfide isomerase (PDI), the application of the camellia glycoside B as an active component in the field of PDI inhibition is proposed for the first time. Experiments prove that low-dose camellia glycoside B can efficiently inhibit PDI activity, and the median inhibitory concentration (IC50) of the camellia glycoside B is 7.3 [mu] M. The discovery provides an important candidate compound for developing a novel PDI inhibitor, has key significance in research and development of drugs for PDI-related diseases such as thrombotic disease prevention and treatment, tumor intervention and the like, and has broad application prospects. Meanwhile, a new direction is opened up for application of natural products in enzyme activity regulation and control.

A system for controlling a vehicle, including one or more communication modules and one or more processors operably coupled to the communication modules. The one or more processors are configured to individually or collectively: receive a geo-fence identifier associated with geo-fence information, where the geo-fence identifier uniquely identifies the geo-fence from other geo-fences; obtain one or more activity regulations for the vehicle based on the geo-fence identifier; and control operation of the vehicle according to the one or more activity regulations.

The invention relates to the technical field of Korean pine nut processing, and provides a Korean pine nut low-temperature gradient humidity control natural opening method based on physiological activity regulation, which comprises the steps of activity retention picking, gradient airing pretreatment, low-damage shelling, double-stage activity drying, low-temperature gradient humidity control opening, physical synergistic opening promotion, natural opening judgment and low-temperature slow frying shaping. The natural opening of the Korean pine nuts in a low-temperature environment is realized through the technologies of segmented humidity control and sun shading, gibberellin-containing airflow drying, ultrasonic wave and magnetic field synergistic opening promotion and the like in combination with an image recognition system to monitor the opening rate. The system integrates the gradient airing module, the low-temperature gradient humidity control cabin and the intelligent control system, and parameters such as temperature, humidity and ultrasonic frequency can be accurately regulated and controlled. According to the method, the aperture opening ratio is larger than or equal to 90%, physiological activity and nutritional ingredients of seeds can be effectively reserved, and processing efficiency and product quality are improved.

A method for preparing and applying a double-sided dura mater sealant capable of regulating fibroblast activity, belonging to the field of medical biomaterials, is disclosed. The double-sided dura mater sealant consists of an A-side facing the dura mater layer and a B-side facing the muscle layer, wherein the A-side facing the dura mater layer comprises a central portion and a peripheral portion. The method includes the following steps: preparing the A-side facing the dura mater layer; preparing the B-side facing the muscle layer; and assembling the double-sided dura mater sealant. This invention possesses the ability to bidirectionally regulate fibroblast activity, both enhancing dura mater fibroblast activity and inhibiting extradura mater fibroblast activity, thus resolving the contradiction in fibroblast activity regulation during dura mater injury repair and extradura mater fibrosis prevention. Simultaneously, it provides reliable cerebrospinal fluid leakage sealing performance, solving the problem of simultaneously achieving dura mater injury repair and extradura mater fibrosis prevention.

The utility model discloses centrifugal equipment for cleaning agents, which relates to the technical field of chemical mechanical equipment and comprises a shell, a driving device and a separating component, a first cavity and a second cavity which are vertically through are arranged in the shell, and the first cavity is connected with the second cavity through a communicating pipe; a feeding hole is formed in the top of the first chamber, a slag discharge hole is formed in the bottom of the first chamber, and a liquid outlet is formed in the bottom of the second chamber. According to the centrifugal equipment for the cleaning agent, provided by the utility model, the efficient separation and purification of the cleaning agent are realized through the synergistic effect of the multi-stage filter screen, the dynamic stirring device, the annular heating plate, the filter element and the intelligent monitoring system. And the design and arrangement of each part are carried out by improving the separation efficiency, optimizing the activity regulation and control capability of the cleaning agent and enhancing the intelligent operation of the equipment, so that the equipment can meet the requirements of various complex working conditions in practical application.