Cell activation dressing with active cell cabin and nutrient communicating channel

Abstract

The invention discloses a cell activation dressing with an active cell cabin and a nutrient communicating channel, and relates to the technical field of focus tissue repair, the cell activation dressing comprises: a patch applied to focus tissue; the cell treatment nursing carrying table is arranged on the patch and acts on the focus tissue; the bionic structure is arranged in the cell treatment care platform deck and is used for guiding and stimulating neogenesis cell proliferation; the activity adjusting input device is communicated with the cell treatment nursing platform through a conduit and is used for providing nutrients for rapid tissue repair and healing in the basic cell therapy; the cell treatment and care platform deck is used as a carrier of a bionic structure, and the activity regulation input device and the metabolic balance output device are used for providing an effective migration and proliferation space for cells, promoting culture solution drainage supply and metabolism-generated waste liquid drainage and removal, effectively guaranteeing activity regulation and metabolic balance, and improving the treatment effect of the cells. And improvement of the chronic / large-area tissue repair efficiency is facilitated.

Description

Technical Field

[0001] The present invention relates to the technical field of lesion tissue repair, and particularly relates to a cell activation dressing having an active cell chamber and a nutrient connection channel. Background Art

[0002] Cell therapy is the most discussed emerging medical treatment in recent years, and the emergence of emerging medical treatments is to solve the limitations of existing treatments.

[0003] Cell therapy is a very broad concept. Common blood transfusions and bone marrow transplants are a type of cell therapy. Simply put, the human body receives blood cells, bone marrow and other human cells into the body to achieve the effect of treating diseases and tissue repair.

[0004] The principle of cell therapy is to take one's own cells (also known as autologous cells) or someone else's cells (also known as allogeneic cells), and after laboratory culture or processing technology, infuse them into the patient's body to achieve the purpose of treating or preventing diseases.

[0005] In cell therapy, autologous cell therapy has a high safety level and a relatively low risk level. That is, cells are taken from the patient himself, and then the cells cultured in a qualified GTP laboratory are re-infused or re-implanted into his own body. The entire preparation process is quite rigorous and must pass the detection of viable bacteria counts before it can be used. Therefore, it is relatively safe to use.

[0006] Currently, in many regions, cell therapy technologies such as autologous immune cells, autologous adipose stem cells, autologous bone marrow mesenchymal stem cells, autologous fibroblasts, and autologous chondrocytes have been clinically available.

[0007] The materials for cell culture and clinical application of "cell therapy metabolic regulation cell platform / system" need to have the following characteristics:

[0008] High biocompatibility, hydration / hydrophilicity, high anti-infectivity, micro-region tissue culture solution / metabolic waste liquid drainage, thermal and structural stability, easy structural design, low pollutant residue, cell migration and proliferation. Currently, it is impossible to meet all these requirements simultaneously, especially:

[0009] 1. Biological materials: Difficult to design the structure, poor mechanical properties, and easy to be infected by biological sources;

[0010] 2. Synthetic materials: Poor biocompatibility of multi-functional groups, high chemical residue toxicity, and high pollution.

[0011] Currently, the clinical bottlenecks in the repair of chronic or large-area tissue (pressure ulcers / diabetic wounds / war wounds) lesion tissues and the use of cell therapy are:

[0012] 1. Chronic / large-area tissue repair: Facing bottlenecks in efficiency / rapid healing.

[0013] 2. The problem of slow healing of chronic / large-area tissue repair can be overcome through the concept of cell therapy.

[0014] 3. In biomedical and clinical tissue healing applications, the need for effective cell migration and proliferation must be faced.

[0015] 4. Requirements for effective cell migration and proliferation: space for effective cell migration and proliferation, drainage and supply of culture medium, drainage and removal of waste liquid generated by metabolism, activity regulation, and metabolic balance.

[0016] At the same time, the soft biomedical materials currently used in clinics can only form a collapsed structure or a non-connected and non-collapsing structure. The collapsed structure or the non-connected and non-collapsing structure cannot provide a suitable space for cell migration and proliferation, and is not conducive to the drainage and supply of culture medium and the drainage and removal of waste liquid generated by metabolism.

[0017] Therefore, in the face of the current technical problems, an active cell therapy care system for the repair of pathological tissues for chronic or large-area tissue repair is applied for in this application. Summary of the Invention

[0018] The object of the present invention is to propose a cell activation dressing with an active cell chamber and a nutrient connection channel in view of the deficiencies in the above technologies, aiming to solve the above problems.

[0019] The present invention provides a cell activation dressing with an active cell chamber and a nutrient connection channel, including:

[0020] A patch, which is applied to the pathological tissue;

[0021] A cell therapy care platform, which is arranged on the patch and acts on the pathological tissue;

[0022] A bionic structure, which is arranged in the cell therapy care platform and is used to guide and stimulate the proliferation of new tissue cells;

[0023] An activity regulation input device, which is connected to the cell therapy care platform through a catheter and is used to provide nutrients for rapid tissue repair and healing of basic cell therapy;

[0024] A metabolic balance output device, which is connected to the catheter and is used for draining and removing the waste liquid generated by metabolism.

[0025] Preferably, the bionic structure includes a three-dimensional cell activation cultivation chamber and a metabolic regulation microchannel. The three-dimensional cell activation cultivation chamber includes an outer wall surface of the three-dimensional cell activation cultivation chamber and a chamber space of the three-dimensional cell activation cultivation chamber. The metabolic regulation microchannel is connected to the outer wall surface of the three-dimensional cell activation cultivation chamber and the chamber space of the three-dimensional cell activation cultivation chamber. An isolation film for isolating the cell therapy care platform from the outside is attached to the patch. A catheter is located between the isolation film and the cell therapy care platform and is connected to the cell therapy care platform.

[0026] Preferably, the nutrient is one or a mixture of more of physiological saline, growth factor solution, glucose solution, amino acid solution, high-oxygen water, high-hydrogen water, and plasma.

[0027] Preferably, it further includes a regulator provided on the catheter for regulating the nutrient flow rate in the catheter. The patch is made of silicone material.

[0028] Compared with the prior art, it has the following beneficial effects:

[0029] In the present invention, by using the cell therapy care platform as the carrier of the bionic structure and relying on the activity regulation input device and the metabolic balance output device, an effective space for cell migration and proliferation is provided, the drainage supply of the culture solution and the drainage and exclusion of the waste liquid generated by metabolism are promoted, the activity regulation and metabolic balance are effectively guaranteed, and it helps to improve the efficiency of chronic / large-area tissue repair. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the drawings in the following description are only the preferred embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0031] Figure 1 Schematic diagram of a cell activation dressing with an active cell chamber and a nutrient connection channel according to the present invention;

[0032] Figure 2 Schematic diagram of the structure of the cell activation dressing according to the present invention;

[0033] Figure 3 Internal schematic diagram of the cell activation dressing according to the present invention;

[0034] Figure 4 Schematic diagram of the preparation method of the cell activation dressing according to the present invention;

[0035] Figure 5 Electron microscope image of the three-dimensional activated cell cultivation chamber according to the present invention;

[0036] Figure 6Electron micrograph of the metabolic regulation microchannel of the present invention;

[0037] Figure 7 Electron micrograph of polyvinyl alcohol material;

[0038] Figure 8 Electron micrograph of HepG2 liver cells growing on polyvinyl butyral (biomimetic type);

[0039] Figure 9 Electron micrograph of HepG2 liver cells growing on polyvinyl butyral (biomimetic type) coated with decellularized collagen material;

[0040] Figure 10 Schematic diagram of the structural stability analysis of the active cell therapy care platform with a biomimetic and easily hydrated three-dimensional activated cell culture chamber;

[0041] Figure 11 Schematic diagram of the thermal analysis and structural stability / low pollutant residue of the active cell therapy care platform with a biomimetic and easily hydrated three-dimensional activated cell culture chamber;

[0042] Figure 12 High-magnification micrograph of residual pollution particles and starch residues caused by current domestic and foreign use processes of similar materials;

[0043] Figure 13 Test diagram of the hydrophilic communication space maintained after hydration of three-dimensional activated cell culture chambers with different pore sizes;

[0044] Figure 14 Diagram showing the culture situation of the cell therapy care platform in a high-concentration mold environment.

[0045] In the figure, 1 - patch; 2 - cell therapy care platform; 3 - biomimetic structure; 31 - three-dimensional cell activation culture chamber; 311 - outer wall surface of the three-dimensional cell activation culture chamber; 312 - chamber space of the three-dimensional cell activation culture chamber; 32 - metabolic regulation microchannel; 4 - active regulation input device; 5 - metabolic balance output device; 6 - catheter; 7 - regulator; 8 - isolation membrane; 9 - nutrient; 10 - lesion; 101 - newly formed tissue cells; 102 - autologous cells; 103 - repair surface; 104 - autologous cell extract; 105 - growth factor solution; 21 - culture dish; 22 - culture medium. Detailed implementation mode

[0046] For a better understanding of the structure of the present invention and the functional features and advantages that can be achieved, the preferred embodiments of the present invention will be described in detail below in conjunction with the drawings as follows:

[0047] Embodiment:

[0048] AsFigures 1 to 4 As shown in the figure, the present invention provides a cell activation dressing with an active cell compartment and a nutrient connection channel, including:

[0049] Patch 1, which is applied to the lesion 10 tissue; specifically, Patch 1 is made of silicone material and has adhesiveness to facilitate fixation on the lesion 10 tissue;

[0050] Cell therapy care platform 2, which is arranged on Patch 1 and acts on the lesion 10 tissue after being cleaned and hydrated, that is, covers the lesion 10 tissue;

[0051] Bionic structure 3, which is arranged inside the cell therapy care platform 2 and is used to guide and stimulate the proliferation of new tissue cells 101;

[0052] Active regulation input device 4, which is connected to the cell therapy care platform 2 through a catheter 6 and is used to provide nutrients 9 for rapid tissue repair and healing in basic cell therapy. The injected nutrients 9 can flush and accumulate new cells in the lesion 10, improving the repair efficiency;

[0053] Metabolic balance output device 5, which is connected to the catheter 6 and is used to drain and remove the waste liquid generated by metabolism. Draining and removing the waste liquid generated by metabolism can enable cells to grow effectively and healthily. The cell activation dressing of the present invention is a self - contained cell - type active cell activation dressing.

[0054] Clinical operation of the cell activation dressing of the present invention:

[0055] Pre - clean and disinfect the lesion 10 tissue (pressure ulcer / burn and scald / diabetic wound / war wound).

[0056] Combine the cell therapy care platform 2 with clean water and cover it on the lesion 10 tissue to provide a bionic and easily hydrated three - dimensional activated cell culture compartment, guiding and stimulating the proliferation of new tissue cells 101.

[0057] Use the active regulation input device 4 to provide nutrients 9 for rapid tissue repair and healing in basic cell therapy.

[0058] Use the metabolic balance output device 5 to regulate the oxygen, carbon dioxide, nutrients 9, metabolism in the cell growth micro - environment, and the drainage supply of the culture solution 22. Draining and removing the waste liquid generated by metabolism can enable cells to grow effectively and healthily.

[0059] Injecting normal saline through the active regulation input device 4 can flush and accumulate new cells in the lesion 10, improving the repair efficiency.

[0060] See Figure 5, showing an electron micrograph of a bionic hydrated three-dimensional activated cell culture chamber. To effectively repair and heal, it is also necessary to consider the delivery barrier, and efficiently drain the above-mentioned external nutrients 9 to the cell growth micro-region through the metabolic regulation microchannel 32. The metabolic regulation microchannel 32 can effectively and unobstructedly regulate the oxygen, carbon dioxide, nutrients, and metabolism in the cell growth microenvironment, that is, the drainage supply of the culture solution 22 and the drainage and exclusion of the waste liquid generated by metabolism are regulated by designing a metabolic balance output device 5. The structure of the cell therapy care platform 2 can enable cells to grow effectively and healthily. See Figure 6 , showing an electron micrograph of the metabolic regulation microchannel 32.

[0061] See Figure 7 , showing an electron micrograph of a polyvinyl alcohol material. It can be seen that the current process of polyvinyl alcohol results in insufficient structural support, which is not conducive to the migration and proliferation of HepG2 liver cells.

[0062] See Figure 8 , showing an electron micrograph of HepG2 liver cells growing on polyvinyl butyral (bionic type). It gives the proliferation and migration of HepG2 liver cells on the active cell therapy care platform 2 of the three-dimensional activated cell culture chamber containing bionic hydrated in the present invention. It can be seen that there is space for cell migration and proliferation, which is conducive to the drainage supply of the culture solution 22 and the drainage and exclusion of the waste liquid generated by metabolism.

[0063] Specifically, Figure 8 shows a material of the cell therapy care platform 2 of the present application. The cell therapy care platform 2 can also use a material with an opening diameter of 700 - 1200 microns and a pore wall not greater than 50 microns.

[0064] See Figure 9 , showing an electron micrograph of HepG2 liver cells growing on polyvinyl butyral (bionic type) coated with decellularized collagen material, that is, the proliferation and migration of HepG2 liver cells on the active cell therapy care platform 2 of the three-dimensional activated cell culture chamber containing bionic hydrated in the present invention in combination with an aid.

[0065] See Figure 10 , showing the structural stability analysis of the active cell therapy care platform 2 of the three-dimensional activated cell culture chamber containing bionic hydrated in the present invention. It is found from the double identification thermal analysis results that there are only weight and energy changes due to the evaporation of water molecules alone in the bionic active cell therapy platform of this case at temperatures below 100 degrees Celsius. No residual substances or decomposition products that would decompose were found at temperatures below 300 degrees Celsius, indicating that the structure of the present invention is clean, safe, and stable, with a high level of clinical safety. The clean, safe, and stable structural space is conducive to cell growth and improves the efficiency of cell therapy.

[0066] See Figure 11, showing the thermal analysis, structural stability, and low pollutant residue of the active cell treatment care platform 2 of the three-dimensional activated cell culture chamber with bionic hydration. A comparison between the current domestic and foreign use process technologies of similar materials and the present invention is given. It can be seen that there are still high-process pollutants residues (a large amount of starch and reactant residues) in the domestic and foreign process technology medical device products, resulting in an increase in defective product recalls (toxicity) and a high risk of clinical infections, and causing mold contamination during storage, greatly increasing the management cost.

[0067] See Figure 12 , showing the high-magnification microscopic observation images of the residual pollution particles and starch residue pollution caused by the current domestic and foreign use process technologies of similar materials. It can be seen that there are relatively many residual pollution particles and starch residue pollution.

[0068] See Figure 13 , showing the test images of the hydrophilic communication space maintained after hydration of the three-dimensional activated cell culture chamber with different pore sizes. After hydration, the metabolic regulation microchannels 32 amplify the siphon and capillary effects (CO3, CO2, nutrients, metabolism), where:

[0069] (A) The diameter is about 300um:

[0070] (A1) Dry type;

[0071] (A2) Semi-wet type;

[0072] (A3) Wet type;

[0073] (B) The diameter is about 500um:

[0074] (B1) Dry type;

[0075] (B2) Semi-wet type;

[0076] (B3) Damp type;

[0077] (C) The diameter is about 900um:

[0078] (C1) Dry type;

[0079] (C2) Semi-wet type;

[0080] (C3) Damp type.

[0081] See Figure 14, which shows the cultivation of the cell therapy care platform 2 of the present invention in a high-concentration mold environment. It can be seen that in a high-concentration mold environment, it can still be maintained without being contaminated for more than 30 days. The molecular structure of the material is simple, reducing reaction residues and having good mold resistance. Comparing with domestic and foreign process technology medical device products in the comparative experiment, it is found that serious black mold contamination occurs within 3 days to one week, which will cause storage mold contamination and greatly increase the management cost. The main reason is that there are still high-process contaminants remaining (a large amount of starch and reactant residues) (see Figure 11 's thermal analysis and Figure 12 's high-power microscopic observation diagram results).

[0082] See Figure 2 and Figure 3 , the bionic structure 3 of the present application includes a three-dimensional cell activation cultivation chamber 31 and a metabolic regulation microchannel 32. Among them, the metabolic regulation microchannel 32 can effectively and unobstructedly regulate the oxygen, carbon dioxide, nutrients and metabolism of the cell growth microenvironment, and can realize the drainage supply of nutrient 9. The three-dimensional cell activation cultivation chamber 31 includes a three-dimensional cell activation cultivation chamber outer wall surface 311 and a three-dimensional cell activation cultivation chamber body space 312. The metabolic regulation microchannel 32 is connected to the three-dimensional cell activation cultivation chamber outer wall surface 311 and the three-dimensional cell activation cultivation chamber body space 312.

[0083] See Figure 1 and Figure 2 , an isolation film 8 for isolating the cell therapy care platform 2 from the outside is attached to the patch 1 of the present application.

[0084] See Figure 1 , the catheter 6 of the present application is located between the isolation film 8 and the cell therapy care platform 2 and is connected to the cell therapy care platform 2.

[0085] See Figure 2 , in order to effectively repair and heal, it is necessary to consider the delivery barrier. Through the metabolic regulation microchannel 32, the external nutrient 9 is efficiently drained to the cell growth micro-region to promote cell growth. Among them, the above-mentioned nutrient 9 is one or more mixtures of physiological saline, growth factor solution 105, glucose solution, amino acid solution, hyperoxic water, hydrogen-rich water and plasma; it can also be autologous plasma and peptide solution of high-order cell therapy, quickly providing hydratable three-dimensional activated cell cultivation chamber newborn tissue cells 101.

[0086] See Figure 1 , the present application further includes a regulator 7 provided on the catheter 6 for regulating the flow rate of the nutrient 9 in the catheter 6.

[0087] As Figure 4 shown, the present invention also discloses a preparation method of a self-contained cell type active cell activation dressing with an active cell chamber and a nutrient 9 connection channel: including the following steps:

[0088] Place the cell therapy care platform 2 carrying the three-dimensional cell activation cultivation chamber 31 and the metabolic regulation microchannel 32 in a culture dish 21 filled with a culture medium 22. Extract the autologous cell extract 104 and apply it to the cell therapy care platform 2, and immerse it in the culture medium 22. Input the growth factor solution 105 into the cell therapy care platform 2, and use the three-dimensional cell activation cultivation chamber 31 as a space for rapid and large-scale cell growth; transform the two-dimensional cell proliferation environment of the culture dish 21 into a three-dimensional cell proliferation environment. Cover the repair surface 103 of the lesion 10 with the cultured active cell dressing; wash the cells proliferating and adhering in the space 312 of the three-dimensional cell activation cultivation chamber body with physiological saline or glucose solution to the repair surface 103 of the lesion 10 to accelerate the repair efficiency.

[0089] The above is only a preferred embodiment of the present invention, and does not impose any form of limitation on the present invention. Any person skilled in the art can make many possible changes and modifications to the technical solution of the present invention by using the above technical content, or modify it into an equivalent embodiment with equivalent changes. Therefore, any changes, modifications, equivalent changes and modifications made to the above embodiments based on the technology of the present invention without departing from the technical solution of the present invention all fall within the protection scope of this technical solution.

Claims

1. A cell activation dressing having an active cell compartment and a nutrient connection channel, characterized in that: include: A patch (1) is applied to the lesion (10) tissue; A cell therapy care carrier (2) is disposed on the patch (1) and acts on the lesion (10) tissue; A bionic structure (3) is disposed in the cell therapy care carrier (2) and is used to guide and stimulate the proliferation of new tissue cells (101); An activity regulating input device (4) is connected to the cell therapy care carrier (2) via a conduit (6) and is used to provide nutrients (9) for rapid tissue repair and healing in basic cell therapy; The metabolic balance output device (5) is connected to the conduit (6) and is used for draining and removing waste liquid generated by metabolism.

2. The cell activation dressing having an active cell compartment and a nutrient connection channel according to claim 1, characterized in that: The bionic structure (3) comprises a three-dimensional cell activation culture chamber (31) and a metabolic regulation microchannel (32); the three-dimensional cell activation culture chamber (31) comprises a three-dimensional cell activation culture chamber outer wall surface (311) and a three-dimensional cell activation culture chamber body space (312); and the metabolic regulation microchannel (32) is connected to the three-dimensional cell activation culture chamber outer wall surface (311) and the three-dimensional cell activation culture chamber body space (312).

3. The cell activation dressing having an active cell compartment and a nutrient connection channel according to claim 2, characterized in that: The nutrient (9) is a mixture of one or more of physiological saline, growth factor solution (105), glucose solution, amino acid solution, high oxygen water, high hydrogen water and blood plasma.

4. The cell activation dressing having an active cell compartment and a nutrient connection channel according to claim 1, characterized in that: It also includes a regulator (7) which is arranged on the conduit (6) and is used to adjust the flow rate of nutrients (9) in the conduit (6).

5. The cell activation dressing having an active cell compartment and a nutrient connection channel according to claim 1, characterized in that: The patch (1) is made of silicone material.

6. The cell activation dressing having an active cell compartment and a nutrient connection channel according to claim 1, characterized in that: The patch (1) is bonded with an isolation membrane (8) for isolating the cell therapy care carrier (2) from the outside.

7. The cell activation dressing having an active cell compartment and a nutrient connection channel according to claim 1, characterized in that: The conduit (6) is located between the isolation membrane (8) and the cell therapy care platform (2), and is connected to the cell therapy care platform (2).

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