46 results about "Cross breeding" patented technology

The invention relates to an artificial cross breeding method of Jinlong hybrid spot new germplasm, which belongs to the technical field of aquatic product distant cross breeding, and comprises the following steps: (1) parent breeding; (2) collecting and storing sperms of epinephelus lanceolatus; (3) carrying out reproductive regulation and ovum acquisition on the epinephelus coioides; (4) artificial preparation and incubation of hybrid fertilized eggs; according to the method, the artificial cross breeding of the female epinephelus coioides and the male epinephelus lanceolatus is realized for the first time, and the new germplasm Jinlong hybrid spots of the epinephelus coioides, which grow fast and are high in survival rate, are cultivated. The body weight of the Jinlong hybrid Epinephelus coioides of 3 months old is increased by 46.45% compared with that of pure Epinephelus coioides; and the survival rate of the new germplasm is increased by 62.50% compared with that of the epinephelus coioides. The industrial problems that artificial spawning of large groupers is difficult, the survival rate of fries is low, and fast-growing new germplasm lacks are effectively solved.

The application discloses a method for breeding Ptereleotris radiata by using siRNA molecules interfering with melanin tyr gene expression, wherein the sequence of the siRNA molecules is shown as SEQ ID NO. 6-35, and the siRNA molecules can interfere with the expression of melanin tyr gene in the skin tissue of Ptereleotris radiata. The siRNA sequence is designed and synthesized by using the melanin gene of Ptereleotris radiata, and a powder injection preparation containing the siRNA is obtained. The powder injection preparation is dissolved and injected into the body of Ptereleotris radiata. The individuals with faded melanin in the skin tissue of Ptereleotris radiata are screened out for breeding of parent fish and cross breeding, and the offspring of Ptereleotris radiata is obtained, wherein the skin tissue traits of the offspring are the same as those of the parent fish. By using the breeding method provided by the application, the melanin in the skin tissue of Ptereleotris radiata can be reduced and stably inherited, a foundation for obtaining Ptereleotris radiata varieties is laid, and the economic value of Ptereleotris radiata is improved.

The utility model belongs to the field of breeding utensils, and particularly relates to a peanut crossbreeding utensil which comprises a utensil box. The top end of the vessel box is sleeved with a vessel cover, the vessel box is in threaded connection with the vessel cover, and a cleaning and draining mechanism is arranged in the vessel box. The cleaning and draining mechanism comprises a groove, and the groove is formed in the bottom end of the vessel box; through the design of the cleaning drainage mechanism, the function of cleaning water drops on the inner wall of the culture vessel is achieved, and the problems that an existing breeding vessel is not provided with an assembly capable of being used for scraping the wall, when many water drops are accumulated on the inner wall of the culture vessel due to the influence of the environment temperature, observation of seedlings by observers is affected, and the survival rate of the seedlings is affected are solved. The problems that when an existing device is used for cleaning water drops on the inner wall, a vessel needs to be manually opened for manual wiping, but seedlings are extremely prone to being damaged in the process are solved, and convenience is improved.

The application belongs to the technical field of agricultural hybrid breeding, and discloses a pumpkin hybrid seed production method using rubber bands to bind and isolate pollination. The application aims to solve the problems of complicated isolation operation, easy flower damage, low efficiency and environmental pollution in the existing pumpkin seed production. The method comprises the following steps: in the afternoon of the day before pollination, a ring-shaped closed loop structure elastic rubber band is stretched and sleeved on the upper part of the female flower corolla of the female pumpkin, the elastic contraction force of the rubber band is used to close the front end of the corolla, and pre-binding isolation is realized; on the day of pollination, the rubber band on the female flower is removed to expose the stigma, and artificial pollination is performed; after the pollination is completed, the same rubber band is sleeved back to the original position immediately to tighten and isolate. Through the standardized process of pre-binding-unbinding-pollination-rebinding, the application has the advantages of simple operation, no flower damage, good isolation effect, single flower operation time of not more than 20 seconds, corolla damage rate of less than 2%, hybridization rate of 99%, low cost of rubber band, less pollution, and significant improvement of pumpkin hybrid seed production efficiency and seed purity.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to sexual gland weight traits of fugu rubripes and application of the SNP molecular marker, and relates to the technical field of biology. According to the invention, association analysis is carried out in combination with the sexual gland heavy character of the fugu rubripes, and the SNP molecular marker significantly related to the sexual gland heavy character of the fugu rubripes is successfully excavated. The SNP molecular marker is used for breeding of improved gonad breeds of fugu rubripes, and compared with traditional phenotype-based selective breeding and cross breeding means, the SNP molecular marker has higher accuracy and detection efficiency and has a wide application prospect.

The invention relates to the technical field of biological breeding, and discloses a crossbreeding method of disease-resistant silkworms. The crossbreeding method comprises the following steps: screening crossbreeding parents of the disease-resistant silkworms, stabilizing the characters of the screened disease-resistant parents of Chinese and Japanese silkworms, and hybridizing the disease-resistant parents of the silkworms after the characters are stabilized, so that the stability of the midgut pyosis resistance characters of the silkworms is improved. According to the midgut-pyosis-resistant bombyx mori obtained by the crossbreeding method of the disease-resistant bombyx mori disclosed by the invention, the resistance of the bombyx mori to midgut-pyosis viruses is successfully improved on the basis of ensuring the practicability. In addition, the cross breeding method disclosed by the invention is simple and high in operability, and breeding can be realized only through hybridization.

The invention provides a breeding method of a triploid flowering Chinese cabbage variety, which comprises the following steps of: (1) hybridizing cabbage type rape serving as a female parent and flowering Chinese cabbage serving as a male parent to obtain a triploid hybrid F1 generation; (2) performing phenotype identification on the triploid hybrid F1 generation, and screening combinations which are early in bolting, synchronous in main stem and lateral branch development, obvious in subgenome heterosis and resistant to clubroot; (3) performing a multi-point planting test on the excellent triploid hybrid F1 to verify the yield and disease resistance of the flowering Chinese cabbage; and (4) breeding a special triploid flowering Chinese cabbage variety for seed production. The triploid flowering Chinese cabbage cultivated by the method has the following outstanding advantages of high yield, high economic value, strong disease resistance, multi-round picking and long market supply period. The method provided by the invention solves the problems of lack of special varieties of traditional brassica campestris and low yield of brassica campestris of a dual-purpose variety of brassica campestris, provides a new technical route for germplasm innovation of brassicaceous vegetables, and has important popularization and application values.

The invention provides an efficient rosa chinensis crossbreeding method based on floral organ characters. The method is characterized by comprising the following steps: (1) selection of breeding parents, (2) determination of the breeding parents, (3) planting management of breeding parent variety plants, (4) determination of breeding female parent variety hybrid pollination plants, (5) pollen collection and pollination, and (6) determination of parent varieties with excellent fertility. Finally, the female parent variety and the male parent variety with excellent fertility are subjected to cross breeding, so that the seed setting rate of cross breeding is increased to 90% or above, the cross breeding efficiency is remarkably improved, the breeding cost is reduced, the female parent variety and the male parent variety with excellent fertility can be successfully inherited and improved, and the economic benefit is increased. And an excellent Chinese rose variety with excellent ornamental value and economic value is provided for the market.

The invention relates to the technical field of sunflower crossbreeding, and discloses a pollination auxiliary device for sunflower crossbreeding, which comprises a mounting mechanism, a barrel, a butt-joint disc fixed at the end part of the barrel, and a circular pollen brush arranged on the inner side of the butt-joint disc; the auxiliary mechanism comprises a rotating part used for driving the circular pollen brush to rotate at a constant speed to uniformly and lightly sweep the pollen on the female parent flower disc column head, a micro-vibration part used in cooperation with the rotating part to slightly vibrate the circular pollen brush to assist the pollen to fall off, and a filtering part used for filtering air entering the cylinder body. The pollination device has the beneficial effects that the rotating part can drive the circular pollen brush to rotate at a constant speed, the problem that the pollination effect is poor due to uneven smearing and unbalanced force during manual operation is avoided, the micro-vibration part can assist pollen to fall off, the pollen utilization rate is increased, the filtering part can filter air entering the cylinder, and the pollination effect is improved. Impurities, miscellaneous pollen and the like in the air are prevented from polluting target pollen, and the purity of cross breeding is guaranteed.

The invention discloses a crossbreeding method for breeding pseudosciaena crocea in spring and summer and breeding pseudosciaena crocea in autumn. The crossbreeding method comprises the following steps: 1) screening high-quality parents of a spring and summer breeding group and an autumn breeding group; (2) adjusting the temperature and the illumination period, simultaneously carrying out nutrient enrichment, and carrying out reproduction regulation on the parent fish so as to synchronize gonad development of the parent fish; 3) performing artificial spawning induction by utilizing exogenous hormone, and performing natural hybrid fertilization or artificial dry-method fertilization according to a hybrid combination of breeding female fish in spring and summer, breeding male fish in autumn and breeding female fish in autumn and breeding male fish in spring and summer; 4, the obtained fertilized eggs are hatched and subjected to fry rearing, and finally the hybrid F1 new germplasm is obtained.The directional controllable hybridization of the two breeding populations is achieved, the genetic diversity of the large yellow croaker is increased, the germplasm degeneration problem is solved, and the filial generation has remarkable advantages in the aspects of growth speed, gonad development and the like.

The invention relates to the technical field of biology, and particularly provides a molecular marker primer pair for identifying a wild type or a mutant of a rice salt-tolerant gene OsSGR1 and application of the molecular marker primer pair. The molecular marker primer pair is composed of primer pairs as shown in SEQ ID NO: 6-8. The primer pair provided by the invention can be used for distinguishing a wild type or a mutant of the rice salt-tolerant gene OsSGR1, is applied to auxiliary breeding of a rice salt-tolerant group, can be used for identifying and accurately distinguishing heterozygous and homozygous genotypes in cross breeding, backcross separation improvement and the like and improving the breeding efficiency, and has important practical significance on high-yield, stable-yield and stress-resistant breeding of rice.

The invention provides a plant male gamete fertility control method and a method for obtaining a male sterile material, and belongs to the technical field of plant breeding. According to the invention, the PPCD1 is taken as a target gene, and the PPCD1 gene is subjected to expression inhibition or gene knockout, so that the PPCD1 gene loses functions, pollen development fails, and the purpose of fertility control is achieved. Therefore, the male sterility material can be created through the method, and the method can be used for creating male sterility materials in cross breeding, creating materials for preventing genetic escape of transgenic plants, creating plant materials without pollen pollution and the like, so that the production efficiency, the economic effect and the social and environmental effects are greatly improved.

The application provides a method for improving flowering uniformity of Calathea alliacea, and belongs to the technical field of agricultural plant cultivation. The method comprises the following steps: (1) culturing Calathea alliacea to a seedling height of 18-20 cm, performing first selection and reservation, and culturing the first selected and reserved plants to obtain first batch of flowering plants; (2) performing second selection 50-60 days after the first selected and reserved plants are started to be cultured, culturing the second selected and reserved plants to obtain second batch of flowering plants; and (3) performing first cross pollination after the first batch of flowering plants flowers, cutting off the tillers that have flowered but failed to successfully cross pollinate from the base, continuing to culture the second batch of flowering plants, and performing second cross pollination after the second batch of flowering plants flowers. The method can make the flowering be advanced by one month through winter management and flowering management in the second year, the uniformity rate reaches more than 90%, the average cross pollination fruit setting rate reaches 70%, and the efficiency and success rate of cross breeding are improved.

The utility model provides a novel crossbreeding device, which relates to the field of breeding, and comprises an incubator main body, three strip-shaped chutes are respectively arranged on the left side surface and the right side surface in the incubator main body, three placing plates are connected in the incubator main body in a sliding manner through six strip-shaped chutes, and a breeding disc is arranged at the upper part of each placing plate; a spraying mechanism and a reciprocating driving mechanism are arranged on the cultivation box main body, and a liquid storage box is mounted at the top of the cultivation box main body; and a liquid level monitoring mechanism is arranged in the liquid storage tank. According to the novel cross breeding device, the reciprocating driving mechanism is arranged, so that the uniformity of a sprayed nutrient solution can be effectively improved, and the breeding quality is improved; according to the novel cross breeding device, the liquid level monitoring mechanism is arranged, so that the service life of the water pump can be prolonged, the phenomenon that the amount of nutrient solution in the liquid storage tank is small can be found in time, and the breeding reliability is improved.

The invention discloses an efficient and accurate wheat parent matching method and system based on the distance between a core germplasm and a genome, and belongs to the technical field of cross breeding. The matching method comprises the following steps: acquiring whole genome SNP genotype data of a breeding population, constructing a core germplasm of which the sample size is smaller than that of an original population but the original genetic diversity is greater than or equal to 95%, analyzing the population genetic structure of the core germplasm, calculating the genome distance between individuals of the core germplasm, and selecting the whole genome SNP genotype data of the breeding population. And screening Top N combinations with maximum and minimum genome distances by taking parents from different genetic subgroups as a primary condition and taking an interval from the top quartile to the maximum value of the genome distance as a primary screening range, and outputting a recommended hybrid combination list. According to the method, quantitative indexes of the genetic population structure and the genome distance are innovatively coupled through an algorithm model, double screening of subpopulation complementation and distance optimization is achieved, decision logic is more scientific, and prediction accuracy is higher.

The utility model relates to an artificial pollination auxiliary device, in particular to a jerusalem artichoke cross breeding auxiliary pollination device which comprises a watering can, the watering can comprises a can body, a filter screen is transversely arranged in the can body, a through hole is formed in the filter screen, a water suction pipe of the watering can penetrates through the through hole, a feeding hole is further formed in the filter screen, and a round pipe is arranged in the feeding hole. The upper end of the circular pipe penetrates out of the kettle body and is communicated with the outside, the circular pipe is closed through a valve, the lower end of the circular pipe is located below the filter screen, and the portion of the circular pipe penetrating out of the kettle body is sealed. According to the application, the distance from pollen to the water inlet end of the water suction pipe of the watering can can be changed through the filter screen, so that more pollen can be sprayed out along with the watering can when the watering can sprays water, and pollination of the pollen is facilitated.

The invention provides an embryo rescue method for hybrid generations of chestnut plants and application of the embryo rescue method, and belongs to the technical field of plant breeding. According to the method, hybrid embryos obtained after hybridization pollination of the female parent Japanese chestnuts and the male parent Chinese chestnuts are subjected to in-vitro culture and rescue, the rescue success rate can be increased through the embryo rescue technology, and the abortion rate of interspecific filial generation embryos is effectively reduced. The invention further provides application of the embryo rescue method for the hybrid generation of the chestnut plants in hybrid breeding of the chestnut plants, a certain platform is provided for increasing germplasm resources of the chestnut plants, creating new varieties and transforming old varieties, and a certain theoretical basis is provided for screening the hybrid generation with excellent quality.

The invention discloses a method for cross breeding of cedrela plants by using a dwarf seed orchard, which comprises the following steps: collecting germplasm resources of cedrela tree species, and selecting orchard building parents from the collected germplasm resources; using branches of the garden building parent as scions to be grafted to the rootstock, building a grafting clone, and cultivating the grafting clone into a parent tree body; establishing a dwarf seed orchard by using the parent tree bodies, and shaping and managing the parent tree bodies; carrying out artificial supplementary pollination on the flowering parent tree bodies so as to hybridize the parent tree bodies; managing the parent tree body after pollination until the parent tree body fruit. According to the method, a bearing mother branch at the top of a parent material with excellent characters is grafted to a stock, the stock is planted in a seed garden after survival, the parent tree body is managed, so that the parent tree body is dwarfed, the crown is developed to be heart-shaped, and the hybrid seed can be obtained more easily through artificial supplementary pollination.

The invention discloses a data feature selection method and system based on an improved crossbreeding algorithm. Obtaining a high-dimensional data set; dividing a feature space in the data set into a plurality of subspaces; a sub-population initialization method based on feature space importance is adopted, a rice population is divided into sub-spaces, an improved cross breeding algorithm is adopted in the sub-spaces for feature selection, and an optimal feature subset is output. Specifically, a subspace division strategy based on symmetric uncertainty is adopted, the importance of the features in the subspace can be rapidly evaluated, and the processing efficiency is improved. In the global search stage, the variation amplitude is adjusted according to the fitness value of the individual, the diversity of the population is enhanced, the global search ability is improved, and it is ensured that the population can be widely explored in the solution space and can fully utilize the existing excellent solutions. In the local search stage, local search is prevented from excessively depending on gradient information by combining a simulated annealing mechanism, and it is ensured that the search process is not prone to falling into a local optimal solution.

The invention belongs to the technical field of forest tree genetic breeding, and particularly relates to application of ethylene glycol bis (2-aminoethyl ether) tetraacetic acid in populus euphratica distant hybridization breeding. The invention provides an application of ethylene glycol bis (2-aminoethyl ether) tetraacetic acid in cross breeding of populus euphratica and populus euphratica. The ethylene glycol bis (2-aminoethyl ether) tetraacetic acid is used for overcoming distant hybridization obstacles of populus euphratica. Compared with the application of exogenous plant growth regulators such as 1-naphthylacetic acid and gibberellin A3, the application method provided by the invention has the advantages that the pollination success rate can be obviously improved, distant hybridization obstacles are overcome, the operation is simple and convenient, and the application method is suitable for being popularized in forestry breeding.

A modified plant suitable for automated crossbreeding, a method of making the same and a corresponding automated crossbreeding method.

The present application relates to the technical field of alfalfa breeding, and particularly relates to a seed grading device for alfalfa cross breeding, which comprises a screen assembly, the screen assembly comprises a screen base and a first screen, one end of the first screen is a screen feeding part, the other end is a screen discharging part, and the part of the first screen close to the screen discharging part is rotationally connected to the screen base; the screen base is provided with an elastic assembly and a baffle, the elastic assembly is used to provide elastic force to support the part of the first screen close to the screen feeding part, so that an included angle alpha is formed between the first screen and the screen base, the first screen is provided with a through hole, the through hole is used for the baffle to pass through, and the part of the baffle on the first screen is used to hinder the flow of raw materials on the first screen. The seed grading device for alfalfa cross breeding can achieve the purpose of simultaneously breeding and removing impurities from a large amount of alfalfa seeds mixed with impurities, and improve the impurity removal effect of alfalfa seed cross breeding.

The invention belongs to the technical field of pineapple crossbreeding, and particularly relates to a molecular marker, a primer and a method for identifying the authenticity of a pineapple YLL and MD2 hybrid. The SNP marker is located at 12504101 of an LG7 chromosome, and the genotype of the SNP marker is A / C. A primer group designed on the basis of the SNP marker can quickly and effectively identify the authenticity of YLL and MD2 hybrids of pineapples, the identification result is accurate and reliable, and genetic breeding of pineapples is facilitated.

A spinacia plant with a low oxalic acid content, in particular a low oxalic acid content that is stable and unaffected by environmental conditions. A method for producing the same, a screening method, and a screening kit. A spinacia plant with a low oxalic acid content having at least one low oxalic acid locus located in a chromosome 4 region; a method for producing the spinacia plant having the low oxalic acid content through cross breeding the spinacia plant having the low oxalic acid content with another spinacia plant. A method for screening spinacia plants with low oxalic acid contents by selecting the spinacia plant having at least one low oxalic acid locus located in the chromosome 4 region. A kit for screening spinacia plants with low oxalic acid contents having a primer or a probe that is specifically bound to the low oxalic acid locus.

The invention belongs to the technical field of corn breeding, and particularly relates to a breeding method of puffed corn for Korean corn popcorn, which comprises the following steps: selecting a parent of a burst type corn single cross breed and a parent of a hard grain type corn or semi-purslane type corn single cross breed, and hybridizing in a female parent-female parent form and a male parent-male parent form; performing backcross by taking the parent of the burst type corn as a male parent, and performing diploid induction to obtain a female parent line induced single grain and a male parent line induced single grain, thereby correspondingly inducing a single plant and performing selfing to obtain a single ear; the method comprises the following steps: sowing seeds into ear rows, carrying out field phenotype identification and screening, selfing, harvesting and testing seeds, carrying out popcorn detection and screening on selected ear row seeds, finally screening out a female parent line selfing line and a male parent line selfing line, and hybridizing to obtain hybrids; hybrid seeds are sown, one hybrid seed is selected, and the puffed corn more suitable for the Korean corn flowers is cultivated.

The invention provides a tremella aurantialba spore cultivation method based on single colony streak cross breeding, and relates to the technical field of edible fungus cultivation. The breeding method comprises the following steps: carrying out suspension hook culture on a tremella aurantialba sporocarp, after spores in the sporocarp are separated out, transferring the spores into a plate culture medium to culture tremella aurantialba single colonies, randomly selecting two tremella aurantialba single colonies, marking out an intersection line on the same clean plate culture medium to carry out cross culture, and inoculating a stereum hirsutum liquid strain as a spawn running assistance, and uniformly smearing on a culture medium, and inducing to produce the agaric. The breeding method provided by the invention solves the technical problems of complex operation, tedious steps, high requirements on equipment and long cultivation time of the existing cultivation method, and realizes the technical effects of high spore cultivation speed, simple operation, low requirements on equipment and capability of meeting the requirements of industrialized production.

The invention relates to the technical field of crop breeding, and particularly discloses a water melon and towel gourd crossbreeding method which comprises the following steps: S1, parent selection and cultivation: taking a high-yield tender variety of water melons and a variety of towel gourds with strong disease resistance and obvious furrows as parents, and performing parent cultivation on the parents; s2, parent hybridization: performing hybrid pollination by taking the water melon as a female parent and the towel gourd as a male parent; or performing cross pollination by taking the towel gourd as a female parent and the water melon as a male parent; or carrying out selfing pollination on the watermelons or the towel gourds; s3, seed cultivation: S3-1, management after pollination; (S3-2) reserving hybrid fruits; and S3-3, collecting the hybridized and self-propagated seeds. According to the method, the feasibility of interspecific hybridization of luffa cylindrica (common luffa) and luffa cylindrica (luffa with ridges) is broken through, the limitation of reproductive isolation is broken through, two different hybrid F1-generation seeds are obtained, the excellent hereditary characteristics, such as high yield, disease resistance, stress resistance and high quality, of the luffa cylindrica and the luffa cylindrica are integrated, and a new variety with comprehensive advantages is cultivated.

The invention provides a cross breeding method for olea europaea, which belongs to the technical field of plant breeding, and ensures sperm motility by limiting the composition of premixed pollen; by coating conductive gel on a stigma and combining with a 20-40kHz ultrasonic field to generate a directional acoustic streaming effect, the growth speed of a pollen tube is increased by 2.1 times, and the pollen tube is accurately guided to an embryo sac to improve the fertilization efficiency; then smearing a sealing agent on the fertilized stigmas to improve the occurrence probability of the hybrid seeds; finally, female parents with infertility and high resistance and male parents with excellent characters are screened, and the occurrence probability of the high-resistance hybrid olive seeds is further increased. According to the hybridization method disclosed by the invention, the culture period of high-quality hybrid varieties is greatly shortened, and new varieties with high resistance can be obtained in a relatively short time.

The invention discloses an InDel marker located on a chromosome 9 of a pig and capable of regulating and controlling the papillary number heterosis of the pig and application of the InDel marker. The nucleotide sequence of the InDel marker is shown as SEQ ID NO: 1 or SEQ ID NO: 2; the site is located between the 79th site and the 80th site from the 5'end of the nucleotide sequence shown in SEQ ID NO: 1, and corresponds to insertion mutation of C between the 11197897 bp and the 11197898 bp on the No.9 chromosome of the international pig reference genome version 11.1. The InDel marker provided by the invention can be applied to crossbreeding of pigs so as to improve the nipple number heterosis of offspring pigs and realize genetic improvement of the pigs, and by breeding the pigs with the InDel marker genotype of T / T, the reproductive capacity of sows can be remarkably improved, and the number of weaned piglets can be increased.