25 results about "Cross breeding" patented technology

The application belongs to the technical field of agricultural hybrid breeding, and discloses a pumpkin hybrid seed production method using rubber bands to bind and isolate pollination. The application aims to solve the problems of complicated isolation operation, easy flower damage, low efficiency and environmental pollution in the existing pumpkin seed production. The method comprises the following steps: in the afternoon of the day before pollination, a ring-shaped closed loop structure elastic rubber band is stretched and sleeved on the upper part of the female flower corolla of the female pumpkin, the elastic contraction force of the rubber band is used to close the front end of the corolla, and pre-binding isolation is realized; on the day of pollination, the rubber band on the female flower is removed to expose the stigma, and artificial pollination is performed; after the pollination is completed, the same rubber band is sleeved back to the original position immediately to tighten and isolate. Through the standardized process of pre-binding-unbinding-pollination-rebinding, the application has the advantages of simple operation, no flower damage, good isolation effect, single flower operation time of not more than 20 seconds, corolla damage rate of less than 2%, hybridization rate of 99%, low cost of rubber band, less pollution, and significant improvement of pumpkin hybrid seed production efficiency and seed purity.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to sexual gland weight traits of fugu rubripes and application of the SNP molecular marker, and relates to the technical field of biology. According to the invention, association analysis is carried out in combination with the sexual gland heavy character of the fugu rubripes, and the SNP molecular marker significantly related to the sexual gland heavy character of the fugu rubripes is successfully excavated. The SNP molecular marker is used for breeding of improved gonad breeds of fugu rubripes, and compared with traditional phenotype-based selective breeding and cross breeding means, the SNP molecular marker has higher accuracy and detection efficiency and has a wide application prospect.

The invention relates to the technical field of sunflower crossbreeding, and discloses a pollination auxiliary device for sunflower crossbreeding, which comprises a mounting mechanism, a barrel, a butt-joint disc fixed at the end part of the barrel, and a circular pollen brush arranged on the inner side of the butt-joint disc; the auxiliary mechanism comprises a rotating part used for driving the circular pollen brush to rotate at a constant speed to uniformly and lightly sweep the pollen on the female parent flower disc column head, a micro-vibration part used in cooperation with the rotating part to slightly vibrate the circular pollen brush to assist the pollen to fall off, and a filtering part used for filtering air entering the cylinder body. The pollination device has the beneficial effects that the rotating part can drive the circular pollen brush to rotate at a constant speed, the problem that the pollination effect is poor due to uneven smearing and unbalanced force during manual operation is avoided, the micro-vibration part can assist pollen to fall off, the pollen utilization rate is increased, the filtering part can filter air entering the cylinder, and the pollination effect is improved. Impurities, miscellaneous pollen and the like in the air are prevented from polluting target pollen, and the purity of cross breeding is guaranteed.

The invention discloses a crossbreeding method for breeding pseudosciaena crocea in spring and summer and breeding pseudosciaena crocea in autumn. The crossbreeding method comprises the following steps: 1) screening high-quality parents of a spring and summer breeding group and an autumn breeding group; (2) adjusting the temperature and the illumination period, simultaneously carrying out nutrient enrichment, and carrying out reproduction regulation on the parent fish so as to synchronize gonad development of the parent fish; 3) performing artificial spawning induction by utilizing exogenous hormone, and performing natural hybrid fertilization or artificial dry-method fertilization according to a hybrid combination of breeding female fish in spring and summer, breeding male fish in autumn and breeding female fish in autumn and breeding male fish in spring and summer; 4, the obtained fertilized eggs are hatched and subjected to fry rearing, and finally the hybrid F1 new germplasm is obtained.The directional controllable hybridization of the two breeding populations is achieved, the genetic diversity of the large yellow croaker is increased, the germplasm degeneration problem is solved, and the filial generation has remarkable advantages in the aspects of growth speed, gonad development and the like.

The invention provides a plant male gamete fertility control method and a method for obtaining a male sterile material, and belongs to the technical field of plant breeding. According to the invention, the PPCD1 is taken as a target gene, and the PPCD1 gene is subjected to expression inhibition or gene knockout, so that the PPCD1 gene loses functions, pollen development fails, and the purpose of fertility control is achieved. Therefore, the male sterility material can be created through the method, and the method can be used for creating male sterility materials in cross breeding, creating materials for preventing genetic escape of transgenic plants, creating plant materials without pollen pollution and the like, so that the production efficiency, the economic effect and the social and environmental effects are greatly improved.

The invention discloses an efficient and accurate wheat parent matching method and system based on the distance between a core germplasm and a genome, and belongs to the technical field of cross breeding. The matching method comprises the following steps: acquiring whole genome SNP genotype data of a breeding population, constructing a core germplasm of which the sample size is smaller than that of an original population but the original genetic diversity is greater than or equal to 95%, analyzing the population genetic structure of the core germplasm, calculating the genome distance between individuals of the core germplasm, and selecting the whole genome SNP genotype data of the breeding population. And screening Top N combinations with maximum and minimum genome distances by taking parents from different genetic subgroups as a primary condition and taking an interval from the top quartile to the maximum value of the genome distance as a primary screening range, and outputting a recommended hybrid combination list. According to the method, quantitative indexes of the genetic population structure and the genome distance are innovatively coupled through an algorithm model, double screening of subpopulation complementation and distance optimization is achieved, decision logic is more scientific, and prediction accuracy is higher.

The invention discloses a method for cross breeding of cedrela plants by using a dwarf seed orchard, which comprises the following steps: collecting germplasm resources of cedrela tree species, and selecting orchard building parents from the collected germplasm resources; using branches of the garden building parent as scions to be grafted to the rootstock, building a grafting clone, and cultivating the grafting clone into a parent tree body; establishing a dwarf seed orchard by using the parent tree bodies, and shaping and managing the parent tree bodies; carrying out artificial supplementary pollination on the flowering parent tree bodies so as to hybridize the parent tree bodies; managing the parent tree body after pollination until the parent tree body fruit. According to the method, a bearing mother branch at the top of a parent material with excellent characters is grafted to a stock, the stock is planted in a seed garden after survival, the parent tree body is managed, so that the parent tree body is dwarfed, the crown is developed to be heart-shaped, and the hybrid seed can be obtained more easily through artificial supplementary pollination.

The invention belongs to the technical field of forest tree genetic breeding, and particularly relates to application of ethylene glycol bis (2-aminoethyl ether) tetraacetic acid in populus euphratica distant hybridization breeding. The invention provides an application of ethylene glycol bis (2-aminoethyl ether) tetraacetic acid in cross breeding of populus euphratica and populus euphratica. The ethylene glycol bis (2-aminoethyl ether) tetraacetic acid is used for overcoming distant hybridization obstacles of populus euphratica. Compared with the application of exogenous plant growth regulators such as 1-naphthylacetic acid and gibberellin A3, the application method provided by the invention has the advantages that the pollination success rate can be obviously improved, distant hybridization obstacles are overcome, the operation is simple and convenient, and the application method is suitable for being popularized in forestry breeding.

A modified plant suitable for automated crossbreeding, a method of making the same and a corresponding automated crossbreeding method.

The present application relates to the technical field of alfalfa breeding, and particularly relates to a seed grading device for alfalfa cross breeding, which comprises a screen assembly, the screen assembly comprises a screen base and a first screen, one end of the first screen is a screen feeding part, the other end is a screen discharging part, and the part of the first screen close to the screen discharging part is rotationally connected to the screen base; the screen base is provided with an elastic assembly and a baffle, the elastic assembly is used to provide elastic force to support the part of the first screen close to the screen feeding part, so that an included angle alpha is formed between the first screen and the screen base, the first screen is provided with a through hole, the through hole is used for the baffle to pass through, and the part of the baffle on the first screen is used to hinder the flow of raw materials on the first screen. The seed grading device for alfalfa cross breeding can achieve the purpose of simultaneously breeding and removing impurities from a large amount of alfalfa seeds mixed with impurities, and improve the impurity removal effect of alfalfa seed cross breeding.

A spinacia plant with a low oxalic acid content, in particular a low oxalic acid content that is stable and unaffected by environmental conditions. A method for producing the same, a screening method, and a screening kit. A spinacia plant with a low oxalic acid content having at least one low oxalic acid locus located in a chromosome 4 region; a method for producing the spinacia plant having the low oxalic acid content through cross breeding the spinacia plant having the low oxalic acid content with another spinacia plant. A method for screening spinacia plants with low oxalic acid contents by selecting the spinacia plant having at least one low oxalic acid locus located in the chromosome 4 region. A kit for screening spinacia plants with low oxalic acid contents having a primer or a probe that is specifically bound to the low oxalic acid locus.

The invention belongs to the technical field of corn breeding, and particularly relates to a breeding method of puffed corn for Korean corn popcorn, which comprises the following steps: selecting a parent of a burst type corn single cross breed and a parent of a hard grain type corn or semi-purslane type corn single cross breed, and hybridizing in a female parent-female parent form and a male parent-male parent form; performing backcross by taking the parent of the burst type corn as a male parent, and performing diploid induction to obtain a female parent line induced single grain and a male parent line induced single grain, thereby correspondingly inducing a single plant and performing selfing to obtain a single ear; the method comprises the following steps: sowing seeds into ear rows, carrying out field phenotype identification and screening, selfing, harvesting and testing seeds, carrying out popcorn detection and screening on selected ear row seeds, finally screening out a female parent line selfing line and a male parent line selfing line, and hybridizing to obtain hybrids; hybrid seeds are sown, one hybrid seed is selected, and the puffed corn more suitable for the Korean corn flowers is cultivated.

The invention provides a tremella aurantialba spore cultivation method based on single colony streak cross breeding, and relates to the technical field of edible fungus cultivation. The breeding method comprises the following steps: carrying out suspension hook culture on a tremella aurantialba sporocarp, after spores in the sporocarp are separated out, transferring the spores into a plate culture medium to culture tremella aurantialba single colonies, randomly selecting two tremella aurantialba single colonies, marking out an intersection line on the same clean plate culture medium to carry out cross culture, and inoculating a stereum hirsutum liquid strain as a spawn running assistance, and uniformly smearing on a culture medium, and inducing to produce the agaric. The breeding method provided by the invention solves the technical problems of complex operation, tedious steps, high requirements on equipment and long cultivation time of the existing cultivation method, and realizes the technical effects of high spore cultivation speed, simple operation, low requirements on equipment and capability of meeting the requirements of industrialized production.

The invention discloses an InDel marker located on a chromosome 9 of a pig and capable of regulating and controlling the papillary number heterosis of the pig and application of the InDel marker. The nucleotide sequence of the InDel marker is shown as SEQ ID NO: 1 or SEQ ID NO: 2; the site is located between the 79th site and the 80th site from the 5'end of the nucleotide sequence shown in SEQ ID NO: 1, and corresponds to insertion mutation of C between the 11197897 bp and the 11197898 bp on the No.9 chromosome of the international pig reference genome version 11.1. The InDel marker provided by the invention can be applied to crossbreeding of pigs so as to improve the nipple number heterosis of offspring pigs and realize genetic improvement of the pigs, and by breeding the pigs with the InDel marker genotype of T / T, the reproductive capacity of sows can be remarkably improved, and the number of weaned piglets can be increased.

The invention relates to the technical field of pollen preservation, in particular to a method for preserving kiwi pollen for a long time, which comprises the following steps: (1) collecting and treating flowers; (2) collecting and dehydrating the pollen; (3) sub-packaging and sealing the pollen; (4) low-temperature cryopreservation; and (5) unfreezing for later use. The kiwifruit pollen can be stably preserved for more than 400 days for a long time and can keep high activity of more than 70%, and the limitation that the preservation period is short and the activity attenuation is fast in the prior art is broken through; meanwhile, the method is easy and convenient to operate, low in equipment requirement and suitable for multiple production scenes, the application cost is remarkably reduced, and reliable pollen guarantee is provided for large-scale cultivation and cross breeding of kiwi fruits.

The present application belongs to the technical field of biological breeding, and provides a distant cross breeding method of a purple mustard line. The present application prepares a new type of purple mustard line through distant cross, introduces the purple trait of Brassica napus into Guangning large mustard, and cultivates a purple leaf and stem type large mustard, thereby obtaining an excellent strain with deep purple leaves, and making up for the shortage of purple mustard variety resources on the market.

The invention discloses a distant hybridization cultivation method of a hexaploid common wheat disease-resistant and stress-resistant variety. The method comprises the following steps: parent screening and determination, cross pollination and fruiting promotion, hybrid immature embryo rescue and test-tube plantlet cultivation, molecular marker-assisted screening, and homozygous stability and comprehensive character identification. The invention relates to the technical field of agricultural crop breeding. The distant hybridization cultivation method of the hexaploid common wheat disease-resistant and stress-resistant variety reaches the parent screening standard, hybridization and embryo rescue parameters, a molecular marker screening system and the like are clear and controllable, and the distant hybridization cultivation method can be popularized to other wheat distant hybridization breeding scenes; a standardized technical normal form is provided for improving wheat characters by using excellent genes of related species such as rye, and the genetic basis and the resource utilization range of wheat breeding are widened.

The invention discloses a cross breeding method and system for tilapia mossambica, and belongs to the technical field of aquatic genetic breeding, the method comprises the following steps: S1, parent pure line breeding; s2, hybrid seed production; s3, fry incubation and cultivation; according to the method, the positive synergistic effect between cold-resistant breeding and sex gene homozygous degree breeding is found and utilized for the first time, and the industrial problem that in traditional tilapia crossbreeding, the male rate is unstable, and stress resistance is difficult to consider at the same time is solved. The male rate of the bred new hybrid line is stably higher than 95%, the 96-hour survival rate in a challenge test at 22 DEG C exceeds 90%, the growth speed is increased by 15% or above, and the three excellent characters are stably and cooperatively expressed in the same hybrid line for the first time.

The present application belongs to the technical field of preparation of excellent commercial seed of economic marine shellfish, and particularly relates to a high-efficiency method for preparing interspecific three-way hybrid of Argopecten genus scallop. The method uses a selected and purified male sterile and female fertile hybrid Argopecten mactrolineatus as a female parent, and a restocked Argopecten purpuratus as a male parent, to produce a "Argopecten mactrolineatus Argopecten purpuratus" three-way hybrid scallop commercial seed with hybrid vigor and outstanding stress resistance. The method can realize large-scale, continuous and efficient hybrid seed production, and has the advantages of simple operation, labor saving, low dependence on personnel, and the like. The hybridization rate is increased from less than 30% in the prior art to more than 90%, and the efficiency of interspecific hybrid seed production and the purity of the seed of the protandrous Argopecten genus scallop are significantly improved.

The application discloses a high-oil peanut breeding method and a molecular marker screening method, and comprises the following steps: preparing a combination by performing distant cross breeding of a female parent of a peanut cultivation material of Yueyou 551 and a wild peanut germplasm PI210553, performing data processing on the basis of obtained offspring materials and taking high-oil germplasms as data reference indexes, and obtaining a series of semi-wild blood high-oil germplasms or super high-oil germplasms. Quality trait QTL analysis and positioning are performed on the basis of the semi-wild blood high-oil germplasms or super high-oil germplasms and a construction process thereof, and the semi-wild blood high-oil germplasms or super high-oil germplasms can be used for widening a genetic basis of cultivated peanuts, improving genetic diversity of the cultivated peanuts and increasing a gene resource library of peanut breeding. Development of marker primers is performed on the basis of related sites, and the marker primers can be used for molecular marker assisted selection breeding. The technical scheme of the application has double important values in theory and application.

The invention discloses a hybrid breeding method for procypris merus and crucian carps, and relates to the technical field of freshwater fish hybridization. According to the method, experience-to-precision crossing of parent matching is realized, a parent combination with the best genetic complementarity and the highest hybridization success rate can be scientifically selected through the multi-character comprehensive scoring model and the hybridization affinity prediction model, the hybridization efficiency and the target character directional polymerization capability are improved from the source, and the method is suitable for large-scale popularization and application. The optimal insemination opportunity is accurately locked through an ovum quality evaluation algorithm, and an optimized insemination program is combined, so that the reproductive disorder of distant hybridization is effectively overcome, and then abnormal embryos are automatically removed in an early stage by utilizing an embryo health degree evaluation model, so that the labor burden is greatly reduced, and the efficiency is improved. And the quality of offspring seed groups is ensured.

The utility model relates to the technical field of fruit tree hybrid breeding, disclose a kind of fruit tree hybrid breeding artificial pollination device, including support box, the bottom of the support box is fixedly connected with main motor, the output of main motor is fixedly connected with second gear, the right side of second gear is fixedly connected with propelling connecting rod, the inner wall sliding connection of propelling connecting rod has rotating rod, the outer wall sliding connection of rotating rod has air pressure rod, the outer wall sliding connection of air pressure rod has air pressure cylinder, the outer wall of air pressure cylinder is fixedly connected with spring, the left side fixedly connected with control powder disc of spring, the top of spring is fixedly connected with powder bin, in the utility model, first by the manual rotation of rocker, under the action of support rod, driving rotating rod and slot gear rotate, and using gear slot drives the rotation of support box, and because main motor is fixed on the inner wall of support box, thereby driving the rotation of entire mechanism.

The application discloses a hybrid breeding method by means of virtual traits. In order to overcome the uncertainty and blindness of the prior art of directly selecting and matching hybrid combinations according to the parent performance, the application comprises the following steps: S1, establishing a basic database comprising a hybrid first generation and corresponding parent performance; constructing a basic trait set and establishing an operation method set; S2, randomly selecting a basic trait and an operation method from the basic trait set and the operation method set to combine and construct a selected virtual trait; S3, calculating the parameters and test results of the practical trait and the selected virtual trait by using the data in the basic database; S4, comparing the calculated parameters and test results with corresponding threshold values to select a target virtual trait for selection or matching of a hybrid combination. The application avoids the influence of hybrid advantage during the selection of parents and the matching of hybrid combinations, accelerates the selection process of parents, reduces the number of matched hybrid combinations, and accelerates the breeding process.

The invention provides a test box for paeonia lactiflora crossbreeding, which comprises a box body, a laminate is arranged in the box body, and the laminate divides the box body into a whole plant cabin and an in-vitro cabin; a motor is arranged on the top surface of the laminate, and a circular bearing platform is arranged at the top end of the motor; the motor drives the bearing platform to rotate; mounting shafts are arranged on the bearing platform, and are uniformly distributed on the bearing platform by taking the circle center of the bearing platform as the center; a fluted disc is rotationally mounted on the mounting shaft; a gear ring is further arranged on the top face of the layer plate, located on the periphery of the bearing platform and meshed with the fluted disc. An air inlet hole and an air outlet hole are formed in the laminate, and an air inlet fan is arranged outside the air inlet hole. By utilizing the test box, the growth of the whole Chinese herbaceous peony plant can be met, a real simulation environment can be provided for in-vitro ovary and immature embryo culture, and the pollen germination rate, the fertilization success rate and the immature embryo survival rate are increased.