722 results about "Cross breeding" patented technology

The invention relates to a method for cross breeding and seedling propagation of paphiopedilum, which comprises the steps of artificial pollination, sterile seeding, strong seedling culture, and test-tube plantlet transplant. After the artificial pollination of paphiopedilum coenospecies, a powdery embryo in hybrid fruits is inoculated to a specially prepared seed germination culture medium for culture; plantlets obtained by the sterile seeding are inoculated to a specially prepared strong seedling culture medium for strong seedling culture; and finally, the test-tube plantlet transplant is performed. The method uses unique culture media, ensures the high germination rate, quick seedling and high seedling quality of paphiopedilum hybrid seeds, has the advantages of high operability, high application value and the like, and overcomes the difficulty that the paphiopedilum hybrid seeds are extremely difficult to germinate in a natural state due to the incomplete development of embryo and other difficulties.

The invention discloses a crossing breeding and feeding method of wild boars, domestic pigs and hybrid pigs. The wild boars are not fit for feeding and popularization for the reasons that the number of the wild boars is limited, the meat is not fine and the skin is thick, and mouthfeel is worse. The wild boars are oestrous seasonably, produce fewer piglets, and are not easy to feed wild boars. However, the interest of people of pursuing venison and changing taste is increasing. The method for domesticating wild boars is a task that people pay attention to. The method includes: choosing long and white sows or big and white sows, carrying out crossing breeding through crossing generation I, backcrossing generation II, horizontal crossing generation I, and side crossing generation II of the sows and wild boars, total 6 generations. 12-16 months are required for one generation; or choosing duroc sows, carrying out crossing breeding through crossing generation I, backcrossing generation I, horizontal crossing generation II within lines, and horizontal crossing generation III among lines of the duroc sows and wild boars, total 7 generations, 12-14 months are required for each generation. The crossing breeding and feeding method of wild boars is used for crossing breeding and feeding.

The present invention relates to methods for determining the genetic causes of certain phenotypes. The present invention further relates to methods for predicting the phenotype of a organism from its genotype. In particular, the methods of the invention relate to the use of compendia of biological response profiles of cells having known genetic mutations for comparisons with the biological response profiles of cells having unknown phenotypes and genotypes. The methods of the present invention are particularly useful for monitoring the success of genetic engineering and cross-breeding of crops and livestock. The present invention further relates to a computer system for comparing biological response profiles to a compendium of biological response profiles and to kits for relating the phenotype of a cell type to its genotype or for predicting the phenotype of a cell type.

The invention discloses a method for breeding a mung bean flowering pollination mutant based on CRI SPR / Cas9 gene editing technology and a special gRNA. The method comprises the following steps of: transferring the CRISPR / Cas9 plasmid of a plant containing a gRNA sequence into a mung bean, and thereby, editing the ORF region of the Cha gene of the mung bean shown in SEQ ID NO. 1, wherein the DNA sequence corresponding to the gRNA is shown in any one of SEQ ID NO. 2 to SEQ NO. 21. According to the invention, a gene for coding the YUCCA protein of the mung bean is edited by using the CRISPR / Cas9gene editing technology, and a new mung bean germplasm with missing keel petals and exposed stigmas and anther is obtained, thus providing a basis for cross breeding of mung beans.

The invention belongs to the technical field of crop cultivation, and particularly relates to a cassava blossoming regulation and control technology. The cassava blossoming regulation and control technology is characterized by comprising selection and preparation of land, plantation management, regulation and control of the blooming period, flower retention, regulation and control of the ratio of female flowers to male flowers, measurement of the pollen activity, pollination experiment and cross breeding. Stable and superior hybrid seeds are obtained by adopting a chemical regulation and control method and a manual agricultural measure regulation and control method. The cassava blossoming regulation and control technology has the characteristics of early blossoming time, high blossoming rate and easiness in technical operation, and is suitable for cross breeding in most cassava planting areas in China.

The invention relates to a poultry breeding technology, in particular to a cultivation method of high-yield native laying chicken. The cultivation method comprises the following steps of: F-series cultivation, backcrossing royal chicken with the synthesis series of parental royal chicken and parental Loman laying chicken, obtaining an underlying group strain containing 75 percent of royal chicken consanguinity, and then carrying out continuous four-generation selective breeding; M-strain breeding cultivation: carrying out cross breeding on the parental royal chicken and the parental Loman laying chicken to obtain an underlying group strain containing 50 percent of the royal chicken consanguinity, and then carrying out continuous four-generation selective breeding; producing commercial generation in matching strains: by taking F strains as parental generation male parent and M strains as parental generation female parent, carrying out sex determination on filial generation by adopting an anal swelling method, wherein the female chicken are commercial-generation laying chicken. The cultivation method can cultivate new species (matching strains) of native laying chicken with high egg yield and excellent egg quality, the egg yield of one commercial chicken after entering into a pheasantry for 72 weeks reaches more than 235 and is improved by 50 percent compared with the egg yield of pure native laying chicken; and the commercial eggs have same appearance quality, protein quality and flavor characteristics with native eggs.

The invention discloses a method for breeding a new species of lean meat type pigs-Shennong No. 1, which includes: A) the erhualian sow as female parent and the yorkshire boar as male parent are in cross breeding to obtain the yorkshire*erhualian cross bred sow; then, cross breeding female parent is in cross breeding with the danish landrace boar to obtain danish*( yorkshire*erhualian) ternary hybrid pigs; B) a fundamental selective breeding group of danish*( yorkshire*erhualian) ternary hybrid pig is formed and selected as zero-generation of Shennong No. 1; C) the boars and sows are mated to obtain piglets, good piglets are selected gradually for seed reservation; D) the good piglets are continuously cultivated until sexual maturity, the outstanding individual female and male pig are reserved as the next generation of swine respectively according to reproductive performance and meat quality as well as carcass performance and growth performance; E) the step C) and D) are repeatedly operated for 4-15 times. The main selective breeding indexes of the Shennong No. 1 pig are based on the reproductive performance and meat quality, the Shennong No. 1 pigs have the advantages of good meat quality, which is manifested in red meat color, good water holding capacity, high intramuscular fat, and good tenderness and the like. Referring to the reproductive performance, the number of the first-born Shennong No. 1 sow is more than ten.

The invention is characterized by that on the basis of choice of parents of basswood generic cross and breeding of interspecfic hybridization, the immature embryo of limited quantities got respectively from normal, reciprocal an rotation cross is engaged in induction and cultivation of embryo somatric cell, produces large quantities of embryo of somatic cell in synchronizing development, and leads to cultivate embryo of somatic cell into whole tree characteristics.

The invention relates to a breeding method of a common mudflat shellfish with stress resistance and high yield, particularly a method of adopting second instar wild type mudflat shellfish as parent to establish a sib family and selecting one family with fastest growth, highest survival rate and highest rate from the first filial generation. By choosing excellent ones for breeding from the first filial generation, the groups of two families with fastest growth and highest survival rate are carried out cross-breeding, the fine strain with highest yield is chosen which is carried out the above breeding, and finally the fine variety with stress resistance and high yield is obtained. The advantage of the method is that the genes with economic character in the family can be purely combined fast, thus obtaining a new variety with high yield, stable yield and stress resistance. The family material can be used for study the hereditary property of mudflat shellfish so as to construct the gene linkage map of the mudflat shellfish.

The invention relates to a method for breeding and feeding a cross breeding pig. The adopted technical scheme is as follows: the method comprises the steps of: (1) selecting a breed or a strain with large number and strong adaptability in the local area as a female parent and selecting the breed or the strain with high growth speed and good carcass quality as a male parent; (2) carrying out cross combination by utilizing two-way crossing and two-way rotational crossing; (3) selecting backup sows in a brood of piglets when the piglets are 28-30 days old; (4) feeding and managing nonpregnant sows and pregnant sows; and (5) strictly preventing epidemic diseases. By utilizing the method provided by the invention, the bred breeding pigs show better heteroses and good piglets with high yield, high survival and high protein can be provided for peasant culturists.

The present invention relates to an excellent-quality chicken maternal strain, in particular to a method for the strain breeding of the dark-shank spotty plumage chicken with dwarf gene, which belongs to the technical field of the dark-shank spotty plumage chicken with dwarf gene. The breeding procedures of the present invention are (1) selecting a cock of Chongren chicken and a hen having the dark-shank partridge in the grandparent hen group of the dwarf yellow-feather chicken as the breeding materials and selecting a normal-shaped cock having the dark-shank partridge character in the F1 generation after the hybridization of the cock and the hen; (2) Selecting the dark-shank partridge normal-shaped cock to be mated with the hen of the dark-shank partridge dwarf yellow-feather chicken and selecting the dark-shank partridge dwarf cock and the dark-shank partridge dwarf hen in the F2 generation; (3) cross-breeding and locking the selected dark-shank partridge dwarf cock and the dark-shank partridge dwarf hen so as to get the present invention after the breeding from generation to generation. Since the body of the maternal-strain chicken is short cultivated through the present invention, the chicken has the outstanding advantages of precocious puberty, high-yield of eggs and less feed consumption and cultivated space saving, which greatly reduces the production cost of the commodity generation chicken and improves the comprehensive benefit of the cultivation.

The invention discloses a breeding method of high-quality double-low disease-resistant high-yield lodging-resistant rape cultivar 'Zhen 2405'. The bred high-quality double-low rape cultivar has the advantages of full and round seed grain, heavy thousand grain weight, black seed grain, good marketability, excellent disease resistance, stable yield, good lodging resistance, good plant-type trait, good maturity performance, consistent maturity and suitability for the mechanized harvest. The method comprises the following steps: A) selecting the early-mature high-yield rapeseed line 'Zhen 9701' as the maternal plant, which has high resistance to Sclerotinia sclerotiorum and lodging and has compact plant-type trait, selecting rape line F1 as the male parent, which has good agronomic characters and high-yield trait, and cross-breeding to obtain a rape line F0; and B) sowing the rape line F0, sowing the excellent individual plants after the two-row sowing of the rape line F0, and self-inbreeding for 6 generations to obtain the high-quality double-low high-yield rape cultivar (Brassica napus) 'Zhen 2405' with excellent resistance to Sclerotinia sclerotiorum and lodging.

The invention relates to the technical field of raise livestock breeding and cultivating, and in particular relates to a method for cross-breeding a special wild boar through a domestic boar and a wild boar. The method comprises the steps of patriarchal breeding, maternal breeding, patriarchal and maternal cross-breeding, and continuous progeny selection, wherein patriarchal breeding and maternal breeding involve first cross and first backcross; patriarchal secondary boar contains 75% of consanguinity of wild boar, and 25% of consanguinity of duroc and has the characteristics of being fast to grow and high in lean meat percentage; maternal secondary boar contains 75% of consanguinity of wild boar, and 25% of consanguinity of Jiangkou radish boar and has the characteristics of growing well on the roughest of forage, and being peaceful, and high in maternity; patriarchal secondary boar and maternal secondary boar which are gained after backcrossing are crossed to obtain first-generation special boar, and then the three-generation selection is performed for the first-generation special bar to obtain a special boar core group that contains 75% of consanguinity of wild boar, 1.25% of consanguinity of duroc, and 12.5% of consanguinity of Jiangkou radish boar. According to the method, the size and appearance of the special wild boar are close to that of the wild boar, and the special wild bar is peaceful and grows well on the roughest of forage; white doar and flower boar descendants nearly do not appear; the special wild boar grows fast, the meat is delicious, and the lean meat percentage reaches 66%; linoleic acid in meat accounts for 8% to the maximum based on the total fatty acid, which is 2 to 3 times that of the common domestic boar, and thus the benefit is brought to human health.

The invention relates to a cultivation method for a central plain table purpose mutton new strain. The method comprises a first step of cross breeding of a dorper ram and a small tailed han sheep ewe, cross breeding of an East Friesian ram and the small tailed han sheep ewe and cross breeding of the East Friesian ram and a dorper ewe to obtain F1 generations which are respectively called Doper-han, East-han and East-Doper, a second step of cross breeding of a East-han ram and a Doper-han ewe in the F1 generations to obtain an F2 generation, a third step of cross breeding of a Doper-han and East-Doper hybridization ram and an East-han and Doper-han hybridization ewe to obtain an F3 generation, a fourth step of athwart cross of the ram and the ewe, and a fifth step of carrying out shutting seed selection and propagation expanding on the athwart cross generation to obtain the central plain mutton new strain. The obtained central plain mutton is high in reproductive rate, and the reproductive rate can reach 220-240%. Growth rate in the early stage is fast, the weight of sheep with age of 0-3 months can reach 200-300g per day, and the weight of sheep with age of 3-6 months can reach 220-370g per day. Dressing percentage is 48-51%, carcass quality is good, and meat quality can reach a special grade. The central plain table purpose mutton new strain is high in adaptability to the central plain environment, and is suitable for barn feeding and lamb production.

The invention relates to method of crossbreeding the exotic fresh water crayfish, especially to method of crossbreeding the Luo shi swamp shrimp. The invention introduces the distant Luo shi swamp shrimp sprout A, mating the female parent shrimp and male parent shrimp of the distant Luo shi swamp shrimp sprout A with that of native breed and getting the cross cut B1+ and negative cross B1-; mating the cross cut B1+ and negative cross B1- with the native breed and getting the backcross B2++ of B1+, negative back cross B2+- of B1+, negative back cross B2-- of B1- and back cross B2-+ of B1-; breeding the backcross B2++ of B1+, negative back cross B2+- of B1+, negative back cross B2-- of B1- and back cross B2-+ of B1-. The survival rate and seed-accumulating rate of the four kinks of sprout is all good, the composition of water, coarse ash content, coarse protein, coarse fat content, amino acid and aliphatic acid is higher than that of native obviously by applying the technique in this invention, especially the breed-accumulating rate of the cross B2-+ of B1- is increased to 67.25%, the rate of emergence to 94.56%.

The invention provides a method for performing mating type gene identification on a basidiospore monocaryon strain which is separated from different hypsizigus marmoreus strains. In the identifying process, in particular application of a flushing reaction, a palisade reaction and transplanting, the method can primarily identify the mating type gene of the hypsizigus marmoreus intuitively and quickly without other instruments and equipment. When the method is applied in cross breeding, the mating type gene of basidiospore monocaryon parent and progeny strains can be identified conveniently so as to improve the purposiveness and the effectiveness of the cross breeding process. The adopted method can shorten the time for identifying the mating type gene for 5 to 10 days, reduce the number of comparing-screening strains by 75 percent theoretically in cross breeding, improve the fecundity, and is more convenient and efficient particularly in application of multiple cross breeding of multiple strains. Then, the method selects the compatible basidiospore monocaryon strain having clamp connection according to the identification result to carry out biparental crossing, and screens hybrid strains according to growth speed, growth potential and economical character index, thereby obtaining stable, high yield and high quality hybrid strains.

The invention belongs to a freshwater fish cross breeding technology in the culture field, and in particular relates to a breeding and culturing method of hybridized crucian carp, which comprises the following steps: (1) selecting parent fish: selecting Pengze crucian carp as a female parent and Ictiobus cyprinellus as a male parent; (2) culturing the parent fish: culturing the female parent and the male parent in separate pools; (3) injecting hormone for induced spawning; (4) carrying out artificial insemination; (5) carrying out abhesion treatment on the oosperm; (6) hatching the oosperm; and (7) culturing fish fry. The improved crucian carp bred and obtained by the method provided by the invention has fast growth, strong stress resistance and good meat quality, the improved crucian carp with good quality can be stably obtained, and the germplasm can not degenerate easily.

The present invention relates to a high-efficient breeding technique of Eurasian raisin grape. After the raisin grape has flowered for 30-50d, the young fruit of the grape is picked. The ovule is taken out in a sterilized state. The embryo is inoculated to an MS culture medium containing different levels of plant growth regulators of gibberellic acid (GA3), indoleacetic acid (IAA) and 6-benzylaminopurine (6-BA) for executing embryo rescue. The average embryo development rate using the breeding technique of the invention is increased from 8.3-11.9% of a patient disclosed in the background art to 84%. The germination rate obtains 75.33% and the seedling rate obtains 74.17%. Furthermore the embryo rescue technique of the raisin grape obtained through the technique of the invention has the advantages of increased seedling rate of offspring, easier obtainment of more filial generation, increased parent selection range of raisin grape cross breeding, accelerated breeding process of new species of the raisin grape and increased breeding efficiency of the raisin grape through increasing the embryo development rate and the germination rate of the raisin grape.

The invention discloses a method used for quickly breaking dormancy of a peony seed epicotyl. With the method, the dormancy of the peony seed epicotyl supposed to naturally germinate in two years can be quickly broken, and the peony seed can germinate smoothly after being processed for three months; under the isolated culture condition, the dormancy characteristic of the peony seed epicotyl can be broken after the processing period of two weeks. With the adoption of the two methods disclosed by the invention, high-quality peony seedlings can be obtained; the peony nursery stock germinating quickly after processing is highly consistent with the phenotype of peony seedlings obtained by the traditional method, in other words, the parental genetic phenotype is maintained, and the seed germination, seedling and flow formation processes are greatly shortened; besides, the method can be used for creating heterogeneous parental new germplasm, so that the efficiency of traditional breeding and cross breeding of peony is improved, and the breeding and genetic improvement processes of peony are shortened.

The invention relates to a new strain of edible fungus, belonging to the technical field of edible fungus development and utilization. The new grey variant strain of edible fungus with red pleurotus: Pleurotus djamor (Rumph.: Fr.)Boedijn, is the new strain of edible fungus selectively bred through single spore cross breeding and has the number No.1933 in CGMCC (China General Microbiological Culture Collection Center). The strain is characterized in that the color of sporophore is stable grey white, the whole sporophore is pure and transparent, the cellulose content is 5-12%, and the strain has favorable mouth feel and is used for producing the edible fungus in the high temperature season. Mycelium grows very fast and has strong ability in fighting bacteria and also has developed aerial hyphae. The hypha has clamp connection and the growth temperature of 3-45 DEG C; the temperature of sporophore differentiation is 18-35 DEG C, and the new grey variant strain is typical high temperature edible fungus. Straws, saw dust, bran, and the like are selected as cultivation materials. The edible fungus is cultivated in March to November. Cultivation materials produced in scale do not need high pressure or normal pressure sterilization, and the sporophore output is 15-25%. The strain is newly selected and bred edible fungus and has application value in the edible fungus field and ornamental fungus field.

The invention discloses a method for breeding a high-quality anti-mite bee variety. The method is characterized by comprising the following steps of: 1) selecting breeding materials, wherein 'jiangshan No.2' Italian bees with relatively high royal jelly yield, France pure Italian bees with high honey production performance and healthy local Chinese bees with high anti-mite performance are selected as species introduction objects according to a series of indexes such as anti-mite performance, productivity and vitality; 2) breeding the anti-mite bee variety, wherein breeding drones are selected from the jiangshan No.2, selecting a bred jiangshan No.2 virgin queen bee according to the requirements of honey bee biology and inheritance breeding characteristics, and performing the artificial fertilization and cross breeding between the breeding drones and the bred jiangshan No.2 virgin queen bee so as to obtain a jiangshan No.2 bee variety with high production performance and disease resistance, using the jiangshan No.2 bee variety as male parents and using the pure Italian bees which are introduced from France and have high honey production performance as female parents; and 3) since breeding starts in spring, detecting and cutting off pupae of the France Italian bees every other 7 days till the breeding ends. The method can solve the problem that bee colonies are jeopardized by bee mites for beekeepers and improve breeding speed of the bee colonies and the yield and the quality of bee products.

The invention provides a Brassica napus annual multi-generation generation adding method. Four stages including seed pretreatment, transplanting and seedling management, flowering phase management and pod development period management are effectively controlled, and one-year three-cropping or four-cropping (ie, annual three-generation or four-generation generation adding) of the Brassica napus is realized, the seed breeding process is effectively accelerated, and the seed breeding year limit is shortened. The controllable greenhouse cultivation technology is adopted, so that the running around at different places are avoided, the time is saved, and manpower and material resources are saved. The Brassica napus annual multi-generation generation adding method is applied to backcross or cross breeding, and seed breeding of novel high-quality and high-yield disease-resistant varieties of Brassica napus can be accelerated.

The invention belongs to the technical field of poultry breeding. A method for breeding a new strain of black-bone chicken with higher production performance comprises the following steps of: step 1, taking parents of foreign broiler chicken as female parents instead of hen CD, and the black-bone chicken with jute feather inland as male parents, and generating a generation F1 through cross breeding, selecting and leaving individuals with jute feather, black bone, and black skin in the generation F1; step 2, crossing and propagating the female individuals and the male individuals obtained in the step 1, and separating out jute feather, recessive white feather, black feather, and reed catkins, and removing recessive white feather, from the colored feather colony through crossing, and purifying and fixing the hair color, black bone, black shin, sexual maturity and other appearance characters according to the family and individual selective method, improving the growing speed, reproductive performance and other economic characters; step 3, through the breeding of four or more generations, successfully breeding the new strain of the black-bone chicken with relatively higher production performance in jute feather, recessive white feather, black feather, and reed catkins. The method improves the early growing speed and egg laying performance of the black-bone chicken in the nation, and obviously reduces the feed / gain ratio.

The invention provides a genetic algorithm by employing guided local search for a multi-objective optimization problem. The algorithm is used for the field of flexible job-shop scheduling. A flexible job-shop scheduling problem belongs to an NP-Hard problem, optimization of multiple objectives often needs to be faced in real production, and the objectives are in interaction and conflict. The genetic algorithm aims at solving the problems of too fast convergence, insufficient population diversity and over-high calculation cost of enumerating all neighborhood solutions caused by continuous cross breeding of close relatives in a genetic operation in the genetic algorithm in the prior art. According to the algorithm, a procedure of calculating a crossover rate and a mutation rate before genetic crossover and mutation and a procedure of searching a movable process and a feasible position by using the guided local search are designed for these problems; and through introduction of the two procedures, the calculation cost is reduced while algorithm premature is avoided. The algorithm is high in practicability and can be well used for actual job-shop scheduling.

The invention provides a cross breeding method for a Pelteobagrus fulvidraco variety namely Huangyou No. 1 with improved growth character. The cross breeding method comprises the following steps: 1, selection of rapidly-growing cross parents: 1.1, selection of a female parent, and 1.2, selection of a male parent; 2, strengthening breeding of parents; 3, artificial parturition hastening; 4, artificial cross fertilization; 5, artificial incubation; and 6, fry breeding. The cross breeding method provided by the invention breeds the first-filial generation of Huangyou No. 1 by cross breeding of female Pelteobagrus fulvidraco and pelteobagrus vachelli; and the first-filial generation of Huangyou No. 1 is tawny in body color, and has extremely significant growth vigor and strong stress resistance.

The invention discloses a gene MS33 related to maize male nuclear sterility and application thereof in cross breeding. The invention provides MS33 protein related to maize male nuclear sterility, which is shown in (a) or (b): (a) protein composed of an amino acid sequence shown in a sequence 1 in a sequence table; or (b) protein which is derived from the sequence 1 by substituting and / or deleting and / or adding one or more amino acid residues on the amino acid sequence of the sequence 1, and related to plant male fertility. MS33 gene mutation causes maize complete male sterility, characters are complete, and environmental influence is avoided. The gene has application potential in maize hybrid seeds.

The invention relates to a method for inducing a high-frequently generating of cross-breeding liriodendron liquid suspended somatic embryo by solid-liquid alternate culture and cross-breeding liriodendron regeneration. After an immature zygotic embryo from ball-shaped stage to cotyledon stage is obtained from separating, and goes through embryonic callus induction stage, embryonic callus propagation stage, the stage of the embryonic callus tissue suspension cell system establishing and pre-multiplication, embryonic suspension cell multiplication stage, the stage of stopping embryonic suspension cell multiplication and forming of embryo, somatic embryo development stage and somatic embryo plant regenerating stage, the regenerated plant of cross-breeding liriodendron can be obtained.

The invention belongs to the technical field of aquatic livestock cross breeding and particularly relates to a cross breeding method for fish of different categories of eleotris oxycephala and oxyeleotris marmoratus. The cross breeding method comprises the steps of selecting of parent fish, intensified breeding of the parent fish, sex discriminating and the sex ratio of the parent fish, artificial induced spawning and inseminating, incubating of fertilized eggs, breeding of fry of 1.2 cm, breeding of fry of the specification of 3 cm, breeding of the fry, breeding of commercial fish and the like. According to the cross breeding method, the artificial control measure is adopted, wide cross is carried out by selecting eleotris oxycephala female parents and oxyeleotris marmoratus male parents, the growth rate and the breeding performance of eleotris oxycephala coeno-species are improved through the cross breeding advantages, the cross-bred filial generation has the advantages of being good in body color, high in growing speed and high in temperature adaptability, and an effective way and the technological support are provided for the large-scale and industrialization development of the cultured eletridae fish new species.