The invention discloses a primer, a kit and a detection method for detecting a recessive white feather locus genotype of a chicken, belonging to the technical field of biology. According to the invention, a specific primer P1 of an allelic gene c is designed according to the fact that 7.7kb insertion (ev1) exists in a fourth intron of a TYR gene, a specific primer P2 of the allelic gene c is designed according to the fact that 7.7kb insertion (ev1) does not exist in a fourth intron of a PMEL17 gene, a primer P3 is a common downstream primer of the primer P1 and the primer P2, a whole genome of a to-be-detected chicken containing the TYR gene is taken as a template, the primers P1, P2 and P3 are put in the same PCR (Polymerase Chain Reaction), an amplified fragment of the PCR is subjected to agarose gel electrophoresis after once PCR, and the recessive white feather locus genotype of the TYR gene of the chicken is identified according to the result of the agarose gel electrophoresis. The method greatly improves the genotype judging efficiency and accuracy, the method is simple, the genotype judging time is short, no sequencing or restriction enzyme is needed, no special instrument is required, the cost is low, and the method is easy to popularize.