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A molecular identification method of chicken recessive white feather gene

A recessive white feather and molecular identification technology, applied in the field of animal genetics and breeding, can solve the problem of long time, and achieve the effect of expanding the size of the group and shortening the breeding time.

Inactive Publication Date: 2011-12-28
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the long time defects of traditional breeding methods, to find a fast and reliable molecular biological method for identification of chicken recessive white feather gene

Method used

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  • A molecular identification method of chicken recessive white feather gene
  • A molecular identification method of chicken recessive white feather gene
  • A molecular identification method of chicken recessive white feather gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Sample preparation and storage

[0019] Take 100ul blood of white-feathered silkies in Yunyang County, Hubei Province, China, containing anticoagulants, and use the commonly used phenol-chloroform crude extraction method (Sambrook J, Fritsch E F, Maniartis T. Molecular cloning Experiment Guide [M]. 2nd Edition. Jin Dongyan, Li Mengfeng. Beijing: Science Press, 1999.465-467) Extract blood DNA from white feather silky chicken, and store the DNA sample in a 4°C refrigerator for later use.

[0020] 2. Primer Design

[0021] The primer design of this example is shown in Table 1.

[0022] Table 1 PCR amplification primers designed by the present invention

[0023] Primer

effect

forward primer

reverse primer

size

primer pair 1

Check for retroviruses

Virus insertion

5'GTTGCCTCT

GGCTCTATT 3'

5' TTTCTGTGAGT

AAGGGTTG 3'

292bp

primer pair 2

Detect if a TYR base

due to control

5'TTGT...

Embodiment 2

[0032] 1. Sample preparation and storage

[0033] Take 100ul blood of white legion chickens containing anticoagulants, and use the commonly used phenol-chloroform crude extraction method (Sambrook J, Fritsch E F, Manny Artis T. Molecular Cloning Experiment Guide [M]. No. 2 Edition. Jin Dongyan, Li Mengfeng. Beijing: Science Press, 1999.465-467) extract blood DNA, and store the DNA sample in a 4°C refrigerator for later use.

[0034] 2. Primer Design

[0035] The primer design of this example is shown in Table 2.

[0036] Table 2 PCR amplification primers designed by the present invention

[0037] Primer

effect

Forward

reverse

size

primer pair 1

Check for retroviruses

Virus insertion

5'GTTGCCTCT

GGCTCTATT 3'

5' TTTCTGTGAGT

AAGGGTTG 3'

292bp

primer pair 2

Detect if a TYR base

due to control

5'TTGTTGGAG

GCTGGGTAT 3'

5'ATGGGCTTGCT

TGAGGTA3'

764bp

[0038...

Embodiment 3

[0046] 1. Sample preparation and storage

[0047] The blood was collected from Xinhua Chicken of Hubei Xinhua Ecological Livestock and Poultry Development Co., Ltd., and 100ul blood containing anticoagulant was used for crude extraction with phenol-chloroform (Sambrook J, Fritsch E F, Maniati Si T. Molecular Cloning Experiment Guide [M]. 2nd Edition. Jin Dongyan, Li Mengfeng. Beijing: Science Press, 1999.465-467) Extract blood DNA, and store the DNA sample in a 4°C refrigerator for later use.

[0048] 2. Primer Design

[0049] The primers used in this example are listed in Table 3.

[0050] Table 3 PCR amplification primers designed by the present invention

[0051] Primer

effect

forward primer

reverse primer

size

primer pair 1

Check for retroviruses

Virus insertion

5'GTTGCCTCT

GGCTCTATT 3'

5' TTTCTGTGAGT

AAGGGTTG 3'

292bp

primer pair 2

Detect if a TYR base

due to control

5'TTGTTG...

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Abstract

The invention belongs to the technical field of chicken molecular marker preparation. Specifically, the invention relates to a molecular identification method of a chicken recessive white feather gene. A molecular identification method for the chicken recessive white feather gene was established, and primer pair 1 and primer pair 2, wherein primer pair 1 is used to detect recessive white feather alleles, and primer pair 2 is used to detect colored feather alleles. The method is: extract the genomic DNA of the chicken to be identified, perform PCR amplification with primer pair 1 and primer pair 2; detect the amplified product by electrophoresis; judge the feather color genotype of the chicken according to the electrophoresis result: if only primer pair 1 amplifies the product , it is a recessive white feather homozygote (the phenotype is white feather); if only primer pair 2 amplifies the product, it is a non-recessive white feather homozygote (the phenotype may be colored or white feather); if both pairs of primers can amplify If the product is produced, it is a recessive white feather heterozygote (the phenotype may be colored or white feather). The invention provides a fast method for identifying the chicken recessive white feather genotype.

Description

technical field [0001] The invention relates to the field of animal genetics and breeding, in particular to a molecular identification method for a chicken recessive white feather gene. Background technique [0002] With the improvement of living standards, people's consumption concept has changed from the quantity type to the quality type. White-feathered chickens are one of the important symbols of imported broilers and some laying hens (such as white-shell hens). Broilers imported from abroad grow fast and have high feed efficiency, but their meat quality is worse than that of local chickens in China. The high-quality chickens (including broiler chickens and laying hens) produced by Chinese local chickens are more and more favored by consumers. Market demand has promoted the development of high-quality chicken breeding. A good breeding material is very important for breeding. Most chicken breeds imported from abroad are of dominant white feather type, and the offspring o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 龚炎长彭秀丽李世军俸艳萍杨桓陈忠
Owner HUAZHONG AGRI UNIV
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