32results about How to "Shorten breeding time" patented technology

The invention discloses an Indel site simultaneously related to the pod number and the single plant yield of soybean, a molecular marker and application of the Indel site and the molecular marker, and belongs to the field of molecular marker-assisted breeding. The Indel site is located at 19bp, 855bp and 388bp of a soybean No.6 chromosome, and the polymorphism is an amplified fragment difference of 89bp. The detection method comprises the following steps: extracting a soybean genome DNA to be identified and carrying out PCR amplification by taking the soybean genome DNA as a template; detecting the size of a PCR amplification product fragment by 1% agarose gel electrophoresis; when the fragment of the amplification product is a small fragment, the soybean to be identified is judged to be a soybean line with multiple pods and high yield, and when the fragment of the amplification product is a large fragment, the soybean to be identified is judged to be a soybean line with small pods and low yield. According to the method, the detection and breeding cost is reduced, the cultivation effect of the soybean high-per-unit-yield variety is improved, the progress of soybean yield improvement is accelerated, and the soybean breeding time is shortened.

The invention discloses a 5-HT1B gene molecular marker related to growth of penaeus vannamei boone and application, and belongs to the technical field of molecular marker-assisted breeding of aquatic animals. The sequence of the molecular marker is shown as SEQ ID NO.3, the molecular marker comprises five sites of E3-157G / A, E3-454C / G, E3-469C / T, E3-478G / C and E3-514G / T, and the dominant genotypes are AA or AG, GG or CG, CT, CG and TT or GT in sequence. The invention further provides a haplotype molecular marker composed of the E3-157G / A and the E3-514G / T, and the haplotype molecular marker comprises the E3-157G / A and the E3-514G The penaeus vannamei feed can be used for culture and improvement of penaeus vannamei; the method is not limited by the development stage, and can provide technical means and theoretical support for early breeding of penaeus vannamei individuals / families / varieties; the method can further shorten the breeding time and reduce the breeding cost.

The invention belongs to the technical field of biology, and discloses a molecular marker closely linked with a snakehead sex gene, which can accurately identify the genetic sex of snakeheads. The invention also provides a specific KASP primer for amplifying the molecular marker closely linked with the snakehead sex gene, which can be used for detecting the genetic sex of snakeheads, is simple and easy to use, has high analysis flux and high accuracy, and is suitable for detecting a large number of samples. The molecular marker provided by the invention has important practical significance for quickly and accurately screening pseudo-female snakehead and super-male snakehead, shortening the breeding time and improving the breeding efficiency.

The invention discloses a detection reagent and method for identifying feather traits of chickens and application. It is found that when the feather phenotype of a chicken is a curled feather, an InDel site exists at 2040242-2040255bp of a chromosome 34 of an Ensembl chicken reference genome CRCg7b version, the deleted nucleotide sequence is shown as SEQ ID NO: 1, the site is a homozygous deletion type, and meanwhile, the SNP site is g.2040608Ggt; and the genotype of A is AA type. The combination of the two sites can be applied to early auxiliary selection of the curled chickens. When the detection reagent is used for detecting the two sites, the feather phenotype of the chicken can be quickly, effectively and accurately predicted, so that the breeding time is shortened, and the breeding process is accelerated.

The present invention belongs to the technical field of molecular genetics, and specifically relates to a SNP site related to the fine wool rate of fine wool sheep and its application. The SNP molecular marker described in the present invention is located at the A / T mutation of the 236304249th nucleotide site on chromosome 1 of the international sheep reference genome Oar_v4.0 version. The present invention also provides the use of a reagent for detecting the above-mentioned SNP site in detecting the fine wool rate trait of fine wool sheep or in molecular marker-assisted breeding of fine wool sheep, which has great application value in fine wool sheep variety screening, variety identification or molecular marker-assisted breeding; it is used for early breeding selection, shortening the generation interval, accelerating the breeding process, saving a lot of breeding costs, and has broad market prospects.

The invention relates to the technical field of garden bonsai manufacturing, in particular to a fraxinus hupehensis branch and trunk pile cultivating method. Comprising the following steps: (1) cutting a pile material; (2) soaking the pile material in water; (3) rooting treatment; (4) arranging piles; (5) shading and moisturizing; (6) on-site maintenance; and (7) shaping and potting. The method is suitable for intervention of the fraxinus hupehensis thick branch piles with the diameter larger than 10 cm, after fraxinus hupehensis piles are soaked in water, the bases of the piles are treated with a rooting agent, and the piles are cut in rows in a culture medium with a certain depth; fraxinus hupehensis stump seedlings which have the branch diameter larger than 10 cm and can be used for manufacturing large stump landscapes and landscape trees are obtained through specific maintenance for one year, the survival rate of the fraxinus hupehensis stump seedlings is increased, the breeding time of fraxinus hupehensis bonsai is greatly shortened, technical guarantee and technical support are provided for rapid forming of the fraxinus hupehensis large stump bonsai and the landscape trees, and the method is suitable for large-scale production of the fraxinus hupehensis large stump bonsai and the landscape trees. The economic benefit of the fraxinus hupehensis is improved.

The invention belongs to the technical field of biological genetic genes and aquatic organisms, and discloses an SNP (Single Nucleotide Polymorphism) marker related to rapid growth traits of Mastacembelus armatus and application of the SNP marker, the SNP marker is shown as SEQ ID NO: 1, and the base site at the 1305th site in a palmitoyl protein thioesterase 1 gene sequence is C or G. The invention also provides a primer pair and a kit. According to the invention, growth traits of a mastacembelus armatus mixed family group are analyzed through whole genome association analysis, an SNP allele related to rapid growth of mastacembelus armatus is determined, and a method for rapidly, accurately and effectively detecting rapid-growing individuals of mastacembelus armatus by using the SNP marker is established. The method can be used for identifying the mastacembelus armatus individuals with excellent growth traits in the early stage so as to shorten the breeding period of the mastacembelus armatus, and is of great significance to industrial screening and cultivation of mastacembelus armatus varieties which grow quickly.

The invention discloses a method for rapidly breeding a new variety of medicinal locust tree, which comprises the following steps: (1) collecting locust tree branches in different regions, grafting in the same region, and keeping the stock age and the later-period management measure consistent; (2) performing continuous regular monitoring, adaptive observation and botanical shape continuous observation after grafting survival; (3) according to monitoring and observation results of 3-5 years, excellent plants with fast growth vigor, strong resistance and obvious biological characters are screened out; (4) determining the rutin content in the sophora flowers of the screened plants, and screening out the plants with high rutin content; and (5) detecting the expression levels of rutin synthesis related genes and degradation genes in the sophora flower buds of the screened plants, and finally determining the sophora flower buds with high synthetic gene expression quantity and low degradation gene expression quantity as excellent varieties. According to the method for rapidly breeding the new variety of the medicinal locust tree, evaluation is more comprehensive, excellent varieties can be screened out more easily, and the breeding time is greatly shortened.

The invention relates to the technical field of fruit tree cultivation, and particularly discloses a cultivation method for improving cold resistance of apple plants, which comprises the following steps: after apples grow to a dormancy period, spraying 80-120mg / L of inositol galactoside solution on the whole tree for 3-4 times every 10-11 days. According to the method provided by the invention, the cold resistance of the apple plants is remarkably improved.

The invention relates to the technical field of plant polyploidy breeding, and provides a method for inducing dandelion chromosome doubling by colchicine, which comprises the following steps: sowing and germinating to obtain seedlings, and coating plant growing points of the seedlings with a colchicine solution for induction treatment to obtain dandelion with doubled chromosomes. According to the technical scheme, the problems that traditional breeding of the alkaline land dandelions is difficult, novel breeding technologies are deficient, good varieties are few, biomass of some high-quality varieties is small, and industrial development of the alkaline land dandelions is affected in the prior art are solved.

The invention belongs to the technical field of biotechnology, and discloses an establishment method and application of a mouse gastric cancer model, which not only can further reveal the biological mechanism of Tibetan in Qinghai to adapt to hypoxia, but also has important reference significance for developing therapeutic target drugs for preventing and treating digestive tract tumor diseases of plateau crowds. According to data provided by NCBI (National Center of Biotechnology Information), the HIF-1alpha gene is located on a chromosome 14, the human-derived HIF-1alpha and the mouse HIF-1alpha both have an encodable transcript, the corresponding protein transcript numbers are respectively NP001834.1 and NP031609.5, and the homology of the human-derived HIF-1alpha and the mouse HIF-1alpha is 95.3% through comparison. The amino acid at the human 582nd site is proline, and the target mutation site of the mouse HIF-1alpha gene is also located at the 582nd site, so that a corresponding gene editing mouse is designed on the site.

The invention discloses a molecular marker closely linked with a channa maculata sex gene, which can accurately identify the genetic sex of channa maculata. The invention further provides a specific KASP primer for amplifying the molecular marker closely linked with the channa maculata sex gene, the genetic sex of channa maculata can be detected, and the primer is simple and easy to use, high in analysis flux, high in accuracy and suitable for detection of a large number of samples. The molecular marker provided by the invention has important practical significance for quickly and accurately screening pseudo-female channa maculata and super-male channa maculata, shortening the breeding time and improving the breeding efficiency.

The invention discloses an SNP molecular marker related to grass carp feed utilization rate and application thereof, and belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding. According to the invention, an SNP (Single Nucleotide Polymorphism) site obviously related to the feed utilization rate of the grass carp is screened out, the SNP molecular marker is positioned at the 51653399 site on the chromosome 2 of the grass carp, the polymorphic site of the SNP molecular marker is G / T, and when the genotype of the site is GG, the feed utilization rate of the grass carp is relatively high. The invention also discloses application of the SNP molecular marker, the amplification primer or the kit in breeding of grass carp with high feed utilization rate. The SNP molecular marker provided by the invention can be used for early breeding of grass carp with high feed utilization rate, greatly reduces the breeding workload, can significantly shorten the breeding time, accelerates the breeding process, improves the breeding efficiency, and reduces the breeding cost.

The invention discloses a breeding method and application of morchella pinnata. According to the method, literature research and technical evaluation are firstly carried out to provide reference for follow-up work; then, research preparation is carried out on a growth site, and a wild morchella conestris collection area is determined; wild fungi are collected to obtain spore powder, quality detection is carried out, and then single conidium is obtained and subjected to pure culture and transfer amplification culture. And then carrying out primary culture test, screening a culture medium formula and culture conditions, producing a secondary strain, and carrying out quality evaluation. And finally obtaining a target strain through an artificial cultivation test. The breeding method has systematicness and scientificity, strictly controls the quality of each link and improves the breeding efficiency. By optimizing the culture medium and culture conditions, it is ensured that morchella conestris is high in growth speed, large in biomass, good in shape and high in stability. The bred morchella conestris is popularized and planted in a large area, and has wide market prospects and economic values.

This invention discloses a method for promoting somatic embryogenesis and maturation in Cryptomeria japonica, belonging to the field of somatic embryogenesis technology. The method involves using immature seed embryos as explants to induce embryogenic callus; transferring the proliferated embryogenic callus to a somatic embryogenesis induction medium for further culture to obtain somatic embryos; transferring the somatic embryos to a somatic embryogenesis maturation medium for dark culture to obtain mature cotyledonary embryos; transferring the mature cotyledonary embryos to a somatic embryogenesis basal medium for light culture to obtain somatic embryonic seedlings; and hardening off the seedlings before transplanting to obtain regenerated plants. The results of this application show that embryogenic callus can be stably obtained from different families, with high somatic embryogenesis maturation capacity and rate, enabling the cultivation of a large number of high-quality seedlings with stable genetic traits in a short time; avoiding the phenotypic segregation problem caused by gene recombination during seed propagation, and ensuring that offspring plants inherit the superior characteristics of the parents.

The invention relates to the technical field of crop breeding, in particular to a pollination method and device for breaking the self-incompatibility of isatis tinctoria, and the method comprises the following steps: selecting plants, trimming, installing an insect prevention cover, spraying, pollinating, removing an insect prevention device and harvesting. The invention provides the pollination method for breaking the self-incompatibility of the isatis tinctoria, the method breaks the self-incompatibility of the isatis tinctoria, realizes self-pollination, improves the breeding effect of the isatis tinctoria, has the characteristics of low production cost, good pollination effect and easiness in operation, can greatly improve the self-pollination efficiency of the isatis tinctoria and obtain a selfing line with higher purity, and can be applied to the field of breeding of the isatis tinctoria. The insect pollination hybridization can be well prevented, the isatis tinctoria breeding time can be saved, and the method has a very high application value in the field of isatis tinctoria breeding.

The present invention relates to the field of genetic engineering technology, and in particular to the application of RBL3 gene in regulating rice immunity level and / or rice disease resistance. The present invention identifies a broad-spectrum disease resistance gene RBL3, which belongs to a member of the CDS gene family and encodes a CDP-DAG synthase, which regulates rice growth and immunity level by regulating rice lipid metabolism activity. By gene editing technology, the RBL3 gene in rice is knocked out, and it is found that rice shows obvious broad-spectrum disease resistance to rice blast and bacterial blight. In addition, the gene is highly conserved in crops, and can be widely promoted in multiple species by means of gene editing. Compared with traditional overexpression of disease resistance genes, it can reduce breeding time, improve crop disease resistance level, yield and quality, break species boundaries, and have stronger universality. It has huge disease resistance breeding application potential and can be widely used in disease resistance research of various crops.

The invention discloses a plant gene single base editing mutant genetic transformation efficient screening method, which realizes co-expression through P2A self-cleavage peptide fusion screening gene and base editing effector fusion gene, prolongs the window for playing the role, improves the base editing efficiency, and further obtains more target base editing mutant plants. Besides, a reconstructed vector skeleton contains a newly added screening gene and an original screening gene, and corresponding screening agents are sequentially used in a plant tissue culture process, so that the tissue culture burden caused by screening of a base editing effector protein high-expression transformation material is overcome.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to rapid growth traits of Yellow River carps and application thereof. The SNP molecular marker is shown as 1), 2) or 3) as follows: 1) the SNP molecular marker is located at the 6206162 site of the 12th chromosome of the Yellow River carps and has a polymorphic base of A / G, 2) the SNP molecular marker is located at the 15000329 site of the 23rd chromosome of the Yellow River carps and has a polymorphic base of G / T, and 3) the SNP molecular marker is located at the 490045 site of the 32th chromosome of the Yellow River carps and has a polymorphic base of C / T; the growth character is the weight of the individual. The SNP molecular marker disclosed by the invention can be used for early breeding of the Yellow River carp with a rapid growth character, so that the breeding workload is greatly reduced, the breeding time can be remarkably shortened, the breeding process is accelerated, the breeding efficiency is improved, and the breeding cost is reduced; the method has important guiding significance for improving the production performance of the Yellow River carps, reducing the cultivation cost of the Yellow River carps and increasing the cultivation income, and is suitable for popularization and application.

This invention discloses a specific DNA fragment, primers, identification method, and application for sex identification in yellowfin tuna. The specific DNA fragment has the following gene sequence: male as shown in SEQ ID NO.1 and female as shown in SEQ ID NO.2. Primers were designed based on the specific DNA fragment sequence for PCR amplification and Sanger sequencing analysis. The genotype at position 219 of the male yellowfin tuna DNA fragment is T / G heterozygous, and at position 292 it is A / G heterozygous. The genotype at position 219 of the female yellowfin tuna DNA fragment is T / T homozygous, and at position 292 it is A / A homozygous. This invention successfully achieves non-lethal sex identification during the fry or juvenile stage, which is beneficial for the implementation of asexual reproduction and seedling selection in yellowfin tuna, and has significant practical value and application potential.

The present application relates to rubber tree seedling breeding field, specifically to a kind of in rubber tree seedling high-efficiency rapid selection high-yield single plant method, comprising the following steps: S1: 2 age rubber tree seedling is sawed, according to the amount of latex flowing out of horizontal sawing surface, preliminary selection is carried out, and preliminary selection seedling is selected;S2: preliminary selection seedling is continued to cultivate 24-48 hours, and on the basis of horizontal sawing surface, continue to cut off 0.2-0.4 cm, according to the amount of latex flowing out of horizontal sawing surface, reselecting is carried out, and reselected seedling is selected;S3: reselected seedling is continued to cultivate 24-48 hours, and on the basis of horizontal sawing surface, continue to cut off 0.2-0.4 cm, according to the amount of latex flowing out of horizontal sawing surface, approval selection is carried out, and approved seedling is obtained;S4: approved seedling is continued to cultivate 20-30 days, and latex is collected for detection, according to the sucrose content of obtained latex, final selection is carried out, and high-yield single plant is obtained.The present application method can be screened at 2-3 age in seedling stage, and the method steps are simple, low in cost, high in screening accuracy.

The invention discloses a method for culturing and transplanting corn transgenic tissue culture seedlings. The method for culturing and transplanting the corn transgenic tissue culture seedlings comprises the steps of immature embryo inoculation, II-type embryonic callus induction, adventitious bud induction, multiplication culture, rooting culture, culture in soil, seedling hardening and transplanting. By adopting the culture and transplanting method provided by the invention, the Zun-dan No.1 corn is used as the explant, and the multiplication culture is carried out under the sterile condition, so that the multiplication is fast, the induction rate is high, the breeding time can be greatly shortened, a large number of tissue culture seedlings can be obtained in a short time, the breeding time is saved, the transgenic breeding process is accelerated, and the method is easy to implement, convenient to operate and suitable for industrial production. A genetic transformation system is easy to establish, technical reference can be provided for exogenous gene transformation and transgenic breeding application of Zun-single No.1 corn, new corn germplasm with beneficial genes can be formed, corn transgenic breeding requirements can be met, the maturing rate of tissue culture seedlings and the seed number of a single plant can be increased, and accordingly planting benefits are improved.

The present application relates to the technical field of molecular markers, in particular to a grape seed germination rate related SNP marker and application thereof. The SNP marker is located at the 482011th (base G or A), 485116th (base C or T), 489064th (base C or T) and 496078th (base A or G) positions of the 4th chromosome gene VIT_04s0008g00560 in the grape reference genome version 23; the dominant haplotype is HAP4. Through the haplotype of the SNP marker site, the high and low of the grape seed germination rate can be accurately and quickly judged at the seedling stage, and the plant does not need to grow fruits. The present application can effectively improve the grape hybrid breeding efficiency, shorten the breeding time, and is suitable for grape genetic breeding work.

The present invention belongs to the field of animal molecular biology DNA marker technology and application technology, discloses molecular markers and applications related to the growth traits of Hehua carp, and specifically discloses SNP molecular markers related to the growth traits of Hehua carp, the sequence of the SNP molecular marker is shown in SEQ ID NO:1, the SNP site is located at the 1001st position from the 5' end of the sequence shown in SEQ ID NO:1, and its polymorphism is A / G. The present invention provides a SNP marker for Hehua carp, by detecting the SNP marker, Hehua carp with better growth traits can be effectively selected under the same breeding conditions, and can be effectively used for molecular marker-assisted breeding of Hehua carp. Then, the genotype of Hehua carp parents can be identified according to actual breeding needs, and the appropriate genotype Hehua carp parents can be selected for breeding, so that Hehua carp offspring (fry) with faster growth can be obtained, and the breeding process of Hehua carp can be accelerated.

The invention discloses a molecular marker of a female sturgeon egg laying amount related gene npffr1 and application of the molecular marker, and belongs to the field of sturgeon genetic breeding and sturgeon molecular marker-assisted breeding. The SNP molecular marker for identifying the egg laying amount of the sturgeons is located at the 78bp position of the npffr1 gene nucleotide sequence fragment; the polymorphic form is A / G, and the genotype of a base mutation site comprises AA, AG or GG. Wherein the female individuals with the genotype of AA belong to high-egg-laying-amount individuals, and the female individuals with the genotypes of AG and GG belong to low-egg-laying-amount individuals. The molecular marker provided by the invention can be used for early breeding of female sturgeon egg laying quantity characters, can be used for screening in multiple female sturgeon groups such as acipenser schustatus, Huso dauricus, acipenser sibiricum and hybrid sturgeon, and can remarkably accelerate the artificial breeding process of breeding of sturgeon varieties with high egg laying quantity.

The invention discloses an SNP molecular marker related to grass carp feed utilization rate and application thereof, and belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding. According to the invention, an SNP (Single Nucleotide Polymorphism) site obviously related to the feed utilization rate of the grass carp is screened out, the SNP molecular marker is positioned at the 51653399 site on the chromosome 2 of the grass carp, the polymorphic site of the SNP molecular marker is G / T, and when the genotype of the site is GG, the feed utilization rate of the grass carp is relatively high. The invention also discloses application of the SNP molecular marker, the amplification primer or the kit in breeding of grass carp with high feed utilization rate. The SNP molecular marker provided by the invention can be used for early breeding of grass carp with high feed utilization rate, greatly reduces the breeding workload, can significantly shorten the breeding time, accelerates the breeding process, improves the breeding efficiency, and reduces the breeding cost.

The present invention belongs to the technical field of molecular genetics, and specifically relates to a SNP site related to the fine wool rate of fine wool sheep and its application. The SNP molecular marker described in the present invention is located at the A / G mutation of the 60455332nd nucleotide site on chromosome 5 of the international sheep reference genome Oar_v4.0 version. The present invention also provides the use of a reagent for detecting the above-mentioned SNP site in detecting the fine wool rate trait of fine wool sheep or in molecular marker-assisted breeding of fine wool sheep, which has great application value in fine wool sheep variety screening, variety identification or molecular marker-assisted breeding; it is used for early breeding selection, shortening the generation interval, accelerating the breeding process, saving a lot of breeding costs, and has broad market prospects.

The present invention relates to the technical field of goat molecular marker screening, in particular to key genes JAK2 Insertion-deletion markers affecting goat growth traits and their applications. The insertion-deletion markers are located in goats JAK2 The second intron region of the gene, the nucleotide sequence is shown in SEQ ID NO.1, and there is a 5bp insertion or deletion of TCCAT at the 295bp from the 5' end. The results of association analysis show that the insertion and deletion marker is related to the chest depth, chest width, weight, body height, and chest circumference index of goats. The insertion and deletion marker can be used as a molecular genetic marker for goat growth traits, thereby accelerating the establishment of a goat population with excellent growth traits and improving the speed of breeding of fine varieties.

This invention belongs to the field of molecular biology and discloses a SNP marker associated with salt tolerance in largemouth bass and its application. This invention obtains an SNP molecular marker related to the strength of salt tolerance in largemouth bass by performing correlation analysis on the salt tolerance of extreme and sensitive individuals. The SNP molecular marker, as shown in SEQ ID NO:1, is located at position 501 and has three genotypes: TT, TG, and GG. The GG genotype largemouth bass exhibits a significantly longer survival time under salinity stress than the TG and TT genotypes. By detecting the genotype of this SNP molecular marker, largemouth bass parents with strong salt tolerance can be accurately selected, which can be effectively used for marker-assisted breeding of largemouth bass.

The invention discloses an STS molecular marker closely linked with mature characters of apple fruits and application of the STS molecular marker, and relates to the technical field of fruit tree molecular marker-assisted selective breeding. In early-maturing apples, the InDel variation site and the flanking nucleotide sequence are shown as SEQ ID NO.1, and in late-maturing apples, the InDel variation site and the flanking nucleotide sequence are shown as SEQ ID NO.2. A primer of the STS molecular marker is designed according to an InDel variation site flanking sequence and is used for marker-assisted selection in breeding, and the morning and evening of the mature period of apples can be predicted in the early stage of seedlings; the primer sequences of the molecular marker are as shown in SEQ ID NO. 3 and SEQ ID NO. 4. (1) InDel sequence variation identified in the invention and used for developing the STS marker is a key reason for causing difference in the mature period of the apple fruits; (2) the STS molecular marker can be used for predicting the morning and evening of the ripening period of the apple fruits; (3) the STS molecular marker found by the invention can guide crossbreeding of apples, screening can be carried out in advance, the breeding time is saved, and the breeding efficiency is improved.