8results about How to "Shorten breeding time" patented technology

The invention discloses an SNP molecular marker related to grass carp feed utilization rate and application thereof, and belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding. According to the invention, an SNP (Single Nucleotide Polymorphism) site obviously related to the feed utilization rate of the grass carp is screened out, the SNP molecular marker is positioned at the 51653399 site on the chromosome 2 of the grass carp, the polymorphic site of the SNP molecular marker is G / T, and when the genotype of the site is GG, the feed utilization rate of the grass carp is relatively high. The invention also discloses application of the SNP molecular marker, the amplification primer or the kit in breeding of grass carp with high feed utilization rate. The SNP molecular marker provided by the invention can be used for early breeding of grass carp with high feed utilization rate, greatly reduces the breeding workload, can significantly shorten the breeding time, accelerates the breeding process, improves the breeding efficiency, and reduces the breeding cost.

This invention discloses a method for promoting somatic embryogenesis and maturation in Cryptomeria japonica, belonging to the field of somatic embryogenesis technology. The method involves using immature seed embryos as explants to induce embryogenic callus; transferring the proliferated embryogenic callus to a somatic embryogenesis induction medium for further culture to obtain somatic embryos; transferring the somatic embryos to a somatic embryogenesis maturation medium for dark culture to obtain mature cotyledonary embryos; transferring the mature cotyledonary embryos to a somatic embryogenesis basal medium for light culture to obtain somatic embryonic seedlings; and hardening off the seedlings before transplanting to obtain regenerated plants. The results of this application show that embryogenic callus can be stably obtained from different families, with high somatic embryogenesis maturation capacity and rate, enabling the cultivation of a large number of high-quality seedlings with stable genetic traits in a short time; avoiding the phenotypic segregation problem caused by gene recombination during seed propagation, and ensuring that offspring plants inherit the superior characteristics of the parents.

The invention discloses a plant gene single base editing mutant genetic transformation efficient screening method, which realizes co-expression through P2A self-cleavage peptide fusion screening gene and base editing effector fusion gene, prolongs the window for playing the role, improves the base editing efficiency, and further obtains more target base editing mutant plants. Besides, a reconstructed vector skeleton contains a newly added screening gene and an original screening gene, and corresponding screening agents are sequentially used in a plant tissue culture process, so that the tissue culture burden caused by screening of a base editing effector protein high-expression transformation material is overcome.

This invention discloses a specific DNA fragment, primers, identification method, and application for sex identification in yellowfin tuna. The specific DNA fragment has the following gene sequence: male as shown in SEQ ID NO.1 and female as shown in SEQ ID NO.2. Primers were designed based on the specific DNA fragment sequence for PCR amplification and Sanger sequencing analysis. The genotype at position 219 of the male yellowfin tuna DNA fragment is T / G heterozygous, and at position 292 it is A / G heterozygous. The genotype at position 219 of the female yellowfin tuna DNA fragment is T / T homozygous, and at position 292 it is A / A homozygous. This invention successfully achieves non-lethal sex identification during the fry or juvenile stage, which is beneficial for the implementation of asexual reproduction and seedling selection in yellowfin tuna, and has significant practical value and application potential.

The present application relates to rubber tree seedling breeding field, specifically to a kind of in rubber tree seedling high-efficiency rapid selection high-yield single plant method, comprising the following steps: S1: 2 age rubber tree seedling is sawed, according to the amount of latex flowing out of horizontal sawing surface, preliminary selection is carried out, and preliminary selection seedling is selected;S2: preliminary selection seedling is continued to cultivate 24-48 hours, and on the basis of horizontal sawing surface, continue to cut off 0.2-0.4 cm, according to the amount of latex flowing out of horizontal sawing surface, reselecting is carried out, and reselected seedling is selected;S3: reselected seedling is continued to cultivate 24-48 hours, and on the basis of horizontal sawing surface, continue to cut off 0.2-0.4 cm, according to the amount of latex flowing out of horizontal sawing surface, approval selection is carried out, and approved seedling is obtained;S4: approved seedling is continued to cultivate 20-30 days, and latex is collected for detection, according to the sucrose content of obtained latex, final selection is carried out, and high-yield single plant is obtained.The present application method can be screened at 2-3 age in seedling stage, and the method steps are simple, low in cost, high in screening accuracy.

The present application relates to the technical field of molecular markers, in particular to a grape seed germination rate related SNP marker and application thereof. The SNP marker is located at the 482011th (base G or A), 485116th (base C or T), 489064th (base C or T) and 496078th (base A or G) positions of the 4th chromosome gene VIT_04s0008g00560 in the grape reference genome version 23; the dominant haplotype is HAP4. Through the haplotype of the SNP marker site, the high and low of the grape seed germination rate can be accurately and quickly judged at the seedling stage, and the plant does not need to grow fruits. The present application can effectively improve the grape hybrid breeding efficiency, shorten the breeding time, and is suitable for grape genetic breeding work.

The invention discloses an SNP molecular marker related to grass carp feed utilization rate and application thereof, and belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding. According to the invention, an SNP (Single Nucleotide Polymorphism) site obviously related to the feed utilization rate of the grass carp is screened out, the SNP molecular marker is positioned at the 51653399 site on the chromosome 2 of the grass carp, the polymorphic site of the SNP molecular marker is G / T, and when the genotype of the site is GG, the feed utilization rate of the grass carp is relatively high. The invention also discloses application of the SNP molecular marker, the amplification primer or the kit in breeding of grass carp with high feed utilization rate. The SNP molecular marker provided by the invention can be used for early breeding of grass carp with high feed utilization rate, greatly reduces the breeding workload, can significantly shorten the breeding time, accelerates the breeding process, improves the breeding efficiency, and reduces the breeding cost.

This invention belongs to the field of molecular biology and discloses a SNP marker associated with salt tolerance in largemouth bass and its application. This invention obtains an SNP molecular marker related to the strength of salt tolerance in largemouth bass by performing correlation analysis on the salt tolerance of extreme and sensitive individuals. The SNP molecular marker, as shown in SEQ ID NO:1, is located at position 501 and has three genotypes: TT, TG, and GG. The GG genotype largemouth bass exhibits a significantly longer survival time under salinity stress than the TG and TT genotypes. By detecting the genotype of this SNP molecular marker, largemouth bass parents with strong salt tolerance can be accurately selected, which can be effectively used for marker-assisted breeding of largemouth bass.