544results about How to "Shorten breeding time" patented technology

Disclosed is a method for super-male fish to mate physiological spawner to build super-male yellow catfish breeding groups, belonging to the fish physiological genetics; the method belongs to the technical field of controlling the sex of fish through artificial sex reversal, screening, test cross and group expanding. The technical problem to be solved is that issues are the super-male fish mates the sex-reversed physiological spawner; the gynogenesis process is eliminated; through the mating between the sex-reversed physiological spawner and the super-male fish or male fish, and the combination of the sex ratio analysis and the test cross technology, the breeding time is shortened. The invention adopts the following technical proposal: artificial spawning inducement and fertilization; sex reversal, namely, the male fish is sex-reversed into physiological spawner; test cross sex reversal, namely, the physiological spawner and the super-male or ordinary male fish are test-crossed, and the physiological spawner and the super-male fish are selected, and the test cross sex reversal comprises the artificial spawning inducement and fertilization; sex ratio analysis, namely, the sex of the candidate fish is confirmed; offspring determination, screening and identification; group expanding, that is, the breeding groups of the super-male fish and the super-female physiological spawner are expanded; and the breeding production of all-male fish, namely, ordinary spawner mates the super-male fish to produce the all-male fish. The method is used for the breeding and mass production of super-male yellow catfish breeding groups.

The invention belongs to the technical field of fruit trees and forestry cultivation, and discloses a method for promoting the lateral rooting of a annual carya illinoensis seed seedling, the root of a sprouting carya illinoensis seed seedling is mechanically cut, and a pair of scissors is utilized to cut the part below the fibrous root; and the seedling which is subjected to mechanical root cutting is transplanted into a nutrition pot, melon seed-shaped pebbles are laid on the bottom of the nutrition pot for 5 centimeters, and a nylon net is cushioned on the melon seed-shaped pebbles. A matrix is put in a container, the seedling is transplanted in a facility which is cold-proof and sun-proof for one month, a furrow which has the width of 13 centimeters and the depth of 20 centimeters is defined in a seedbed of a land, and the row spacing of the furrow is 30 centimeters. A layer of non-woven fabric is laid at the bottom of the furrow, a chemical rooting-resistant agent is coated on the non-woven fabric, and the nutrition pots which are planted with the seedlings are put on the non-woven fabric according to the planting distance of 20 centimeters, and are fixed finally. The water and fertilizer management period is normally carried out. The method can be used for effectively promoting the lateral rooting of the carya illinoensis seed seedling.

The invention discloses an ecological breeding method of a boer goat, and relates to the technical field of ecological breeding of goats. The method mainly comprises the following steps: (1) constructing a goat shed; (2) performing ranch management; (3) preparing a feed; (4) feeding pregnant ewes; (5) performing postnatal management on the pregnant ewes; (6) managing for the growing period; (7) managing the goat shed; (8) performing epidemic disease prevention and control. According to the growth characteristic of the boer goat, a scientific rational feeding scheme is made aiming at nutrition requirements of different growth periods, the investment cost is reduced, the growth speed is improved and the culture time is shortened on the basis of guaranteeing that the meat is fresh and qualified, and higher economic benefit is brought to farmers.

The invention discloses a rice blast resistance gene Pi7 and application thereof, and provides a nucleotide sequence of the rice blast resistance gene Pi7 and an amino acid polypeptide sequence coded thereby. The nucleotide sequence comprises two genes which belong to members of a coiled-coil nucleotide binding site leucine-rich repeat (CC-NBS-LRR) resistance gene family and are constitutive expression genes. The invention also provides application of the gene to the aspect of transforming rice or breeding disease-resistant varieties of other plants and application of a molecular marker produced according to the gene sequence to breeding.

The present invention discloses a method for artificial breeding and rearing frog in pens. Said method includes the following steps: selecting seed, egg deposition, incubation, tadpole culture, rearing froglet and adult frog. Besides, said invention also provides the concrete requirements of every step.

The invention relates to a technical method for high-efficient breeding of wheat, which comprises low-temperature vernalization, south breeding and generation adding, shuttle breeding, multi-point combined identification and the like. In particular, the technical method comprises the following steps of: treating the seeds obtained after the strong-winterness wheat blossoms for 15 to 20 days by hydrogen peroxide, washing the seeds by pure water, sprouting the seeds at the room temperature, putting the seedlings growing to 1 to 2 centimeters in an artificial weather room, performing the green-body vernalization of the seedlings for 45 and 50 days at a temperature of between 3 and 5 DEG C and a relative humidity of 70 percent and under a light intensity of 15001x; hardening the seedlings of the winter wheat undergoing the vernalization treatment at the normal temperature for 1 to 2 days, and transporting the seedlings to Yuanmou in Yunnan province; and preparing a rice seedling bed, watering and soaking the rice seedling bed, discharging visible water before transplanting, and transplanting the seedlings in mud at dusk. The disease resistance, stress resistance, adaptability and yieldstability selections of breeding materials are carried out by shuttle breeding and multi-point combined identification and the like. The technical method for breeding provides rich reserve of raw materials for the rapid improvement of the wheat variety, and also lays a sold foundation for demonstration and popularization of a new variety in large areas.

The invention discloses a seedling culturing and planting technology of carya illinoensis and relates to the field of planting of fruit trees. The planting method comprises the following steps: (1) constructing a foundation; (2) accelerating germination and breeding; (3) grafting; (4) managing grafted seedlings; and (5) carrying out field management. By reasonably selecting carya illinoensis breeding, grafting and after-survival management methods, a lot of high-quality seedlings are obtained and sufficient nutrient supply of stocks is guaranteed; the time of breeding nursery stocks through a grafting technology is greatly shortened; dwarfing and shorted juvenile phases of walnut trees are realized and the walnut trees are fruited in advance.

The invention discloses a selective breeding method of a high-resistance tobacco variety and belongs to the technical field of resistive breeding of tobacco. According to the selective breeding method, the tobacco high-yield varieties Yunyan 85, Yunyan 87 and Zhongyan 100 or K326 are taken as recurrent parents, and the cold-resistant variety Honghua Dajinyuan, the drought-resistant variety Zhongyan 14, the brown spot and blackleg resistant variety Zhongyan 86, the root-knot nematode-resistant variety TI1706, the common mosaic virus-resistant variety Taiyan No.7 and the tobacco bacterial wilt-resistant variety D101 as resistant resource parents, and the seven major drought, cold and disease resistant resource genes of the tobacco are pyramided and inserted into the good-quality high-yield tobacco variety with the same genetic background by use of a synchronous backcross and convergent cross method, so that a new high-resistance, high-yield and good-quality tobacco variety, which is highly resistant to 7 drought, cold and disease damage factors and highly consistent with the original recurrent parents in other agricultural and yield traits, is obtained.

The invention relates to a tissue culture, rapid seedling formation and ecological application method for Louisiana iris, which comprises the following steps: 1) material selection: flowers, flower buds, floral axis, blades, roots and other vegetative organs of Louisiana iris are selected as explants; inoculation is carried out after routine disinfection; 2) bud induction and proliferation culture: the sterilized vegetative organs in 1) are inoculated on an induction medium; when callus has an area of 1 to 2 cm2, and forms a 0.5 to 0.8cm green convex block, the vegetative organs are transplanted in a differentiation medium; when the length of the vegetative organs reaches 5 to 8cm, the vegetative organs are transplanted in a rooting medium; 3) root induction: rootless seedlings with consistent growth are transplanted to a root induction medium; when the length of the rootless seedlings reaches 12 to 15cm, seedling adaptation is performed; 4) seedling adaptation: seedling adaptation is carried out for 6 to 8 days under natural light, then seedling emergence can be realized; culture medium at the root section of the seedlings is washed off; the seedlings are transplanted to a sterilized seedling container equipped with mixed substrate; after watering, shading and heat insulation, seedling emergence can be realized. The method has the advantages of fast seedling formation and good quality, and the seedlings can be directly applied to ecology.

The invention discloses a high strength selecting and cultivating method of excellent stichopus japonicus seeds. The high strength selecting and cultivating method comprises the steps of carrying out cultivation, spawning or spermiation promotion, and hybridization on parent stichopus japonicuses from at least four different geographical groups, and carrying out selective breeding at different degrees in different periods according to a quantitative genetics method. By combining two traditional and effective methods of hybridization breeding and selective breeding, the invention preserves excellent properties of the stichopus japonicus and also avoids inbreeding; because the cultivated stichopus japonicuses have excellent properties of different groups, the cultivated stichopus japonicuses have the advantages of fast growth speed, strong stress resistance and the like; and the invention not only realizes seedling pre-culturing of the stichopus japonicus to meet market demand in advance, but also prolongs an effective growth period of the stichopus japonicus within a year, shortens cultivation time, and improves economic benefit; therefore, the inveniton is suitable for large-scaled factory production.

The invention discloses a culturing method for goats. The method is characterized by comprising goat cot preparation, feeding and stocking, epidemic disease control, and goat cot management, wherein the goat cot preparation includes building a goat cot and a playground on a high-terrain and dry place with good ventilation and drainage; feeding and stocking includes respectively feeding goats in morning and evening every day, and breeding the goats outside in the afternoon; the epidemic disease control includes disinfecting the goat cot and the playground by quick lime or plant ashes at regular intervals, to prevent various diseases; the goat cot management includes cleaning the goat cot frequently, eliminating droppings, and paying attention to ventilation, to keep the cot clean and dry, and eliminating various mosquitoes and other insects. The method reduces investment cost, improves growing speed and shortens breeding time on the basis of ensuring delicious and qualified meat quality, and brings about better economical benefits for farmers. In addition, Chinese medicine agentia in fodder can prevent and control epidemic diseases, and security is high, and is beneficial for improving quality of mutton.

The invention discloses a stage pig raising method. The method comprises the following stages of roasters, piglet conservation, growth and fattening. Feeding is performed according to the requirement for nutrients of the stages of pig breeding, the suitable growth environment is provided, the body quality and immunity of pigs are enhanced, the appetite of the pigs is improved, the meat factor of the pig meat quality is improved, and the breeding time is shortened.

The invention discloses a seedless grosvenor momordica with high mogroside content, high whole fruit utilization rate and good taste, and a cultivating method for the seedless grosvenor momordica. The steps of the method are as follows: 1) cultivating and planting the diploid female and male seedlings of the grosvenor momordica; 2) when a male plant emerges a flower bud, inducing the formation of diploid big pollen; 3) picking the male flower that is induced to bloom and screen a diploid big pollen therein; 4) hybridizing the haploid oocyte of the diploid female plant after treating the diploid big pollen in the pollen germination solution, acquire the hybrid seeds after the female plant fruits; 5) propagating the hybrid seed into whole plant and then screening a triploid plant with the Chromosome number inspection; 6) planting the triploid plant and the diploid male plant, and carrying out artificial pollination to the male triploid plant with the male diploid plant when the plant is blooming, thus acquiring the seedless grosvenor momordica when the female plant fruits.

The invention discloses a method for building a high-efficiency regeneration system of superior corn self-bred line agriculture line 531, belonging to the field of plant genetic engineering and transgenosis breeding. The invention takes an agriculture line 531 rataria as an explant, induces in a callus induction medium and produces an II-type embryonic callus; the II-type embryonic callus is subjected to embryoid induction under light in an embryoid induction medium to produce a green embryoid; then, the green embryoid is transported to a regeneration medium and is cultured into a regeneration plant under light; root induction is carried out in a rooting medium, and acclimatization is carried out in Hogland nutrient solution to ensure that a new thick root grows on the root of the regeneration plant; the root is transplanted to nutritional soil for rejuvenation culture; and finally, the root is transplanted to a land for growing field crops to normally grow and seed. The regeneration technology is suitable for the superior corn self-bred line agriculture line 531 with high application value, can ensure that the superior quality of agriculture line 531 corn can be inherited in the corn transgenosis breeding process, and has an important meaning for functional genome group research.

The invention relates to a breeding method of hybrid cabbage, in particular to a simplified breeding method for preparing hybrid cabbage by using cytoplasmic male sterile lines, which belongs to the technical field of plant culture. The invention directly adopts the cabbage varieties of Zhonggan No.22 and Hugan No.2 prepared by cytoplasmic male sterile lines sold in the market as maternal plants, and adopts cabbage inbred lines (with the same ball shape as the maternal plants, the same type of leaf ball mature performance as the maternal plants and stable botanical characters) as pollen suppliers for preparing good coenospecies. The invention has the advantages that when the advantages of using the cytoplasmic male sterile lines for preparing the cabbage coenospecies are maintained, good cabbage inbred lines do not need to be used as recurrent parents for fifth to seventh generation backcrossing and transformation with male sterilant materials, the labor is saved, the cost is saved, the breeding time is greatly shortened, and in addition, the hybrid vigour parts of the original varieties can be maintained into the new hybrid varieties.

The invention discloses a method for continuously breeding artificially-collected larvae of ruditapes philippinarum. The method mainly includes the following steps: (1) a natural sea beach is selected to serve as a continuous breeding field; (2) a breeding beach range is separated from the periphery through plastic boards to prevent the ruditapes philippinarum from escaping and facilitate feeding; (3) pre-disinfection treatment is carried out on the breeding beach; (4) the collected or purchased larvae of the ruditapes philippinarum are evenly put into the breeding field in a high-density mode; (5) feed is immediately put after the ebb, and a thin water layer is kept for five hours; (6) inner-field surface residuals should be completely washed away through seawater; (7) when the adult ruditapes philippinarum is harvested, the ruditapes philippinarum in the sizes meeting the requirement is screened through a screen, and larvae, with the number the same as the number of the screened-out adult ruditapes philippinarum, of the ruditapes philippinarum are additionally put into the field. By means of the method for continuously breeding the artificially-collected larvae of the ruditapes philippinarum, continuous breeding is carried out through the artificially-collected larvae, the natural larvae of the ruditapes philippinarum can obtain sufficient feed and the natural environment, growing is rapid, the breeding time is greatly shortened, and the ruditapes philippinarum can be rapidly supplied to the market.

The invention provides a high active induction culture method for lily bulbil which is characterized in that: through an induction culture of embryoid and tissue enrichment culture of embryoid tissue, a relative high rapid propagation velocity is obtained; then through an differentiation culture of bulb-lets and an intumescence culture of bulb-lets, transplanting bulb-lets of a circumferential diameter bigger than 3cm are obtained. The growth rate of the bulb-lets of the invention can reach more than one million grains per years which is ten times that of conventional methods. With a large number of small bulb-lets obtained, the ratio of bulb-lets with large specification and large grain size is increased. The invention optimizes the culture procedures of preferential lily seedball, shortens the propagation time of preferential lily seedball; furthermore, the field planting survival rate of bulb-lets is high and the production cost is low; the invention is suitable for the scale rapid propagation production of cut lily elite.

The invention relates to a breeding method for a rapid growing line of seawater pearl shells, which comprises the steps of: taking a certain population of Pinctada martensii Dunker as a basic population, firstly selecting individuals with shell width being 10-40 percent higher than average value from the basis population by taking shell width as a selection index, then selecting individuals with shell height or weight being 10-60 percent higher than average value as breeding parents, dissecting the breeding parents, taking sexual glands to conduct artificial insemination, selecting and reserving preparative parents at a young shell stage according to a proportion of 20-30 percent, and finally conducting more than three generations of continuous selection to obtain the rapid-growing large excellent Pinctada martensii Dunker line (variety). The breeding method has the advantages that the method is simple, convenient and feasible, the cost for nursing the parents can be reduced, the growth speed and the individual size of the Pinctada martensii Dunker can be effectively improved and the pearl producing effect is improved.

The invention discloses a buoyant-raft net cage sea cucumber culture device and a sea cucumber culture method. The device comprises a floating ball, a buoyant raft hanging rope and a net cage, wherein two ends of the buoyant raft hanging rope are connected with the floating ball; the net cage is connected with the buoyant raft hanging rope via a lifting rope; the floating ball is connected with a wood pile fixed on the seabed respectively by steady lines; and the buoyant raft hanging rope is long enough to cause the net cage to touch the seabed. In the culture device, because the floating ball is fixed by the steady lines connected with the wood pile fixed on the seabed and the net cage is fixed on the seabed in the culture process, influence on the culture system because of ocean current and sea water fluctuation can be effectively lowered, which is more favourable for the sea cucumber to grow. In the culture process, technicians can pull or put down the net cage by the buoyant raft hanging rope; the buoyant-raft net cage sea cucumber culture device is convenient to operate, effectively lowers culture cost and improves economic benefit.

The invention discloses a method for quickly breeding fritillaria cirrhosa bulbs. The method mainly comprises the steps that fritillaria cirrhosa flakes are utilized for breeding, and fritillaria cirrhosa plants are cultivated quickly through dormancy break treatment, disinfection treatment, flake differentiation treatment and the like. The flakes are utilized for the breeding; the dormancy break treatment is conducted by hormones in a breeding process; the cultivation time is shortened; a tissue culture method is not adopted; in this case, requirements on a technology and an environment are reduced greatly; the cost is lowered; in the breeding process, each small flake can differentiate 4-12 small bulbs; in this case, use ratios of the two-stage and three-stage fritillaria cirrhosa are increased greatly; and the breeding efficiency of the fritillaria cirrhosa is improved.

The invention discloses a PCR primer pair used for identifying the genetic sex of Pseudobagrus ussuriensis, and a rapid identification method thereof. The identification method comprises the steps of examined individual tissue drawing, genome DNA extraction, sex specific DNA fragment PCR amplification, agarose gel electrophoresis, ultraviolet light detection shooting and examined individual genetic sex determination. High-quality genome DNA is extracted through an optimized process, a sex specific DNA fragment is amplified, and the existence or not and the existence characteristic of the sex specific fragment are detected under ultraviolet lights, so the genetic constitution of Pseudobagrus ussuriensis is identified, and the genetic sex is discriminated in 1-2d. The PCR primer pair and the rapid identification method provide a rapid identification method for identifying sex reversal fish and super-male fish, provide technical support for breeding all-male fish, and have the advantages of breeding time shortening, great increase of the output, breeding cost reduction, and profound economic and social values.