2results about How to "Increase genetic diversity" patented technology

The invention relates to an Ectoine-producing halophilic bacterium as well as a screening method and application thereof, and belongs to the technical field of microorganisms. According to the Ectoin-producing halophilic bacteria disclosed by the invention, the Ectoin-producing halophilic bacteria are Vreelandelassp., and the Ectoin-producing halophilic bacteria are Ectoin-producing halophilic bacteria. The 16S rRNA of the Ectoin-producing halophilic bacteria has at least 90% of nucleotide sequence consistency with that of SEQ ID NO. 1; the Ectoin halophilic bacteria are used for expressing 2-aminobutyric acid acetyltransferase, 2-aminobutyric acid transaminase and Ectoin synthetase. Through an efficient screening method and deep genome analysis, the novel halophilic strain capable of synthesizing the Ectoine is successfully screened out, and the gene cluster for synthesizing the Ectoine is excavated, so that the production efficiency and yield of the Ectoine can be improved, a microbial resource library is enriched, and the genetic diversity is increased.

The application provides a gene structure variation combination, primers and application thereof significantly related to a Brassica napus growth period, belongs to the field of plant molecular breeding and gene editing technology, and the gene structure variation combination comprises one or more of "DEL00146035 gene structure variation + INS00146036 gene structure variation", DEL00006200 gene structure variation, "DEL00018831 gene structure variation + INS00018834 gene structure variation". BnaC6.MED13 As a major genetic locus (initial flowering time, final flowering time), the gene structure variation combination is respectively composed of BnaA2.GA20ox2 , BnaA4.SPL15 A gene structure variation combination of a genetic locus (silique maturation time) can accurately and quickly and accurately identify a short growth period of a rapeseed variety.