9results about How to "Increase genetic diversity" patented technology

The invention belongs to the field of biological engineering and biological detection, discloses a rabbit-derived Abeta1-40 monoclonal antibody, and a pharmaceutical composition, a detection reagent or a kit containing the monoclonal antibody, and also discloses an application of the monoclonal antibody in preparation of the pharmaceutical composition, the detection reagent or the kit. The rabbit monoclonal antibody prepared by the invention can efficiently and specifically recognize human A beta 40. Compared with mice or rats, the rabbit-derived monoclonal antibody disclosed by the invention is simpler in structure, has higher affinity and is more easily humanized.

The invention discloses a method for creating a high-frequency introgression Chinese cabbage-cabbage introgression line, and relates to the technical field of introgression breeding. According to the method, tetraploid Chinese cabbage and diploid cabbage are subjected to interspecific hybridization to obtain an allotriploid hybrid (AAC), then the AAC is subjected to hybridization and selfing with diploid Chinese cabbage, and a Chinese cabbage-cabbage introgression line with a high-frequency introgression exogenous species genome sequence is created in combination with free microspore culture. According to the invention, the heterotriploid hybrid mediated genetic system is utilized to realize interspecific high introgression, and a new method is provided for breaking the bottleneck of low exogenous species introgression frequency.

According to the method, firstly, the Fujian oyster black shell diploid strain and the Fujian oyster gold shell diploid strain which are pure in shell color and fast in growth are obtained through population breeding, and then the new diploid Fujian oyster strain which is fast in growth speed and high in survival rate is obtained through cross breeding. Secondly, the improved tetraploid Fujian oyster shell black strain which is fast in growth, high in genetic diversity and high and stable in tetraploid multiplying power is obtained in a group breeding and family breeding mode. And finally, hybridizing the obtained diploid Fujian oyster new strain as a female parent and the tetraploid Fujian oyster shell black improved strain as a male parent to breed a fast-growing high-survival-rate triploid Fujian oyster improved strain. The method has the excellent characteristics of high survival rate, high growth speed, high yield and the like, is suitable for being popularized and cultured in southern coastal areas such as Fujian and Guangdong in China, and has a wide industrial application prospect.

The invention relates to an Ectoine-producing halophilic bacterium as well as a screening method and application thereof, and belongs to the technical field of microorganisms. According to the Ectoin-producing halophilic bacteria disclosed by the invention, the Ectoin-producing halophilic bacteria are Vreelandelassp., and the Ectoin-producing halophilic bacteria are Ectoin-producing halophilic bacteria. The 16S rRNA of the Ectoin-producing halophilic bacteria has at least 90% of nucleotide sequence consistency with that of SEQ ID NO. 1; the Ectoin halophilic bacteria are used for expressing 2-aminobutyric acid acetyltransferase, 2-aminobutyric acid transaminase and Ectoin synthetase. Through an efficient screening method and deep genome analysis, the novel halophilic strain capable of synthesizing the Ectoine is successfully screened out, and the gene cluster for synthesizing the Ectoine is excavated, so that the production efficiency and yield of the Ectoine can be improved, a microbial resource library is enriched, and the genetic diversity is increased.

The invention discloses an urban plain ecological environment change monitoring method based on small mammal survey. The method comprises the following steps: firstly, determining a long-eared owl pill collection point, and then obtaining the coverage area proportion, biomass and vascular plant species richness of forests, shrubs, grassland and cultivated land in a predation range area; then calculating biological diversity index measurement parameters based on the long-eared owl food pills: species richness of predated animals, occurrence frequency of the predated animals in the food pills, predation intensity of the predated animals and biomass proportion of the predated animals; and finally, calculating a comprehensive index of the ecological environment quality of the urban plain area based on the indexes to determine the ecological environment change condition of the urban plain area. By using the method, the problem that the biological diversity of the small mammals cannot be observed for a long time is solved, and a technical support is provided for observing and judging the comprehensive quality change condition of the urban ecological environment for a long time by virtue of the small mammals with a relatively good indication effect.

The invention provides saccharomyces cerevisiae with high freeze-thaw tolerance as well as a screening method and application thereof, and relates to the technical field of microbial breeding. Two wild-type saccharomyces cerevisiae and a laboratory saccharomyces cerevisiae with a clear genetic background are selected as parent strains, and the parent strains are repeatedly subjected to the steps of spore production, random mating, repeated freeze thawing and the like to obtain the saccharomyces cerevisiae with high freeze thawing tolerance. The saccharomyces cerevisiae disclosed by the invention has high freeze-thaw tolerance, is intersected with a parent strain, the freeze-thaw tolerance is improved by 7.5 times, and the saccharomyces cerevisiae has ethanol tolerance and high-temperature tolerance. Through random mating of spores of different strains, the genetic diversity of the diploid is increased, and the probability of mutation of the diploid in the directional screening process is increased, so that compared with a parent strain, the freeze-thaw tolerance of the obtained strain is improved by 7.5 times, and the strain can tolerate ethanol with the concentration of 30% and a high-temperature environment of 50 DEG C.

The invention discloses a marker for efficiently identifying alfalfa chromosomes and application thereof, belongs to the field of bioinformatics and molecular cytogenetics, and solves the problems that alfalfa is high in ploidy, large in number of chromosomes, small in chromosomes, lack of obvious symbolic characteristics and high in identification difficulty. The marker for efficiently identifying the alfalfa chromosome is used for identifying chromosomes 1-8 of alfalfa, the marker of the alfalfa chromosome is located on the chromosomes 1-8 of the alfalfa, and the marker of the alfalfa chromosome is composed of six pairs of alfalfa specific sequence amplification primers; the marker for efficiently identifying the alfalfa chromosomes can efficiently and accurately identify the 1-8 chromosomes of the alfalfa, can identify the 1-8 chromosomes in the alfalfa at one time, can quickly distinguish different chromosomes, greatly shortens the identification time, improves the working efficiency, is beneficial to understanding the genetic diversity of the alfalfa, and has good application prospects. Individuals with excellent characters can be conveniently selected for hybridization.

The invention discloses an automatic pollination system for factory strawberry production, which comprises a main controller, a timing control module, a temperature and humidity sensor and a plurality of groups of pollination units, each pollination unit comprises a rack and an air blower, the air blower is connected with a blast pipe, a plurality of cultivation grooves are arranged on the rack side by side, and the cultivation grooves are communicated with the main controller. An air outlet hole is formed in the bottom of a blast pipe installed above the cultivation groove and connected with a short pipe and a hose, a reset mechanism is arranged on one side of the short pipe, a plunger rod in the reset mechanism slides in a reciprocating mode to control opening and closing of the air outlet hole, a cleaning mechanism is arranged on one side of the plunger rod, and when the cleaning mechanism is started, collected objects in the blast pipe are discharged. Meanwhile, the plunger rod is pushed to enter the short pipe to slowly close the air outlet hole; through the linkage design of the cleaning mechanism and the reset mechanism, automatic cleaning of the air blowing pipeline and intelligent control of the air outlet holes are achieved, collected pollen is discharged through the cleaning opening, natural pollination is achieved, it is avoided that ventilation is hindered due to accumulation of the air blowing pipe, and the pollination efficiency and the fruit quality are improved.

The application provides a gene structure variation combination, primers and application thereof significantly related to a Brassica napus growth period, belongs to the field of plant molecular breeding and gene editing technology, and the gene structure variation combination comprises one or more of "DEL00146035 gene structure variation + INS00146036 gene structure variation", DEL00006200 gene structure variation, "DEL00018831 gene structure variation + INS00018834 gene structure variation". BnaC6.MED13 As a major genetic locus (initial flowering time, final flowering time), the gene structure variation combination is respectively composed of BnaA2.GA20ox2 , BnaA4.SPL15 A gene structure variation combination of a genetic locus (silique maturation time) can accurately and quickly and accurately identify a short growth period of a rapeseed variety.