The application provides a
gene structure variation combination, primers and application thereof significantly related to a
Brassica napus growth period, belongs to the field of
plant molecular breeding and
gene editing technology, and the
gene structure variation combination comprises one or more of "DEL00146035 gene structure variation + INS00146036 gene structure variation", DEL00006200 gene structure variation, "DEL00018831 gene structure variation + INS00018834 gene structure variation". BnaC6.MED13 As a major genetic locus (initial
flowering time, final
flowering time), the gene structure variation combination is respectively composed of BnaA2.GA20ox2 , BnaA4.SPL15 A gene structure variation combination of a genetic locus (
silique maturation time) can accurately and quickly and accurately identify a short growth period of a
rapeseed variety.