Gene QDTY 2.9IR66897B capable of obviously increasing rice reproductive-stage drought tolerance and molecular marker method thereof

A technology of IR66897B and molecular markers, applied in biochemical equipment and methods, DNA/RNA fragments, genetic engineering, etc., can solve the problem of lack of molecular markers for drought-tolerant molecular assisted selection breeding, etc., so as to facilitate hybridization and transfer, and accelerate The effect of the breeding process

Inactive Publication Date: 2016-10-26
AGRI GENOME INST OF SHENZHEN CHINESE ACADEMY OF AGRI SCI +1
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Problems solved by technology

However, OsPIP1-3 was cloned by reverse genetics, and no molecular markers have been developed that can be directly used in molecular-assisted selection breeding for drought tolerance.

Method used

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  • Gene QDTY 2.9IR66897B capable of obviously increasing rice reproductive-stage drought tolerance and molecular marker method thereof
  • Gene QDTY 2.9IR66897B capable of obviously increasing rice reproductive-stage drought tolerance and molecular marker method thereof
  • Gene QDTY 2.9IR66897B capable of obviously increasing rice reproductive-stage drought tolerance and molecular marker method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Mapping of Drought Tolerance QTL

[0017] 1. Test materials

[0018] Firstly, three Southeast Asian landraces IR66897B (P1 population male parent), MR77 (P2 population male parent) and MR167 (P3 population male parent) screened from the germplasm resource bank were constructed with the Chinese high-yield, high-quality, drought-tolerant japonica rice Jijing 88, respectively. 3 high-generation backcross introduction line groups. After two rounds of drought stress selection in the reproductive period, 72 drought-tolerant lines from 3 families were selected to form a selective breeding population (Table 1).

[0019] Table 1. Composition information of 3 selective breeding populations

[0020]

[0021] N 1 is the original BC without drought stress 2 f 2 Group size; N 2 is the coefficient of drought-tolerant plants selected after the first round of drought stress treatment; N 3 is the coefficient of drought-tolerant plants selected after two rounds of drought st...

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Abstract

The invention relates to a gene QDTY 2.9IR66897B capable of obviously increasing rice reproductive-stage drought tolerance and a molecular marker method thereof, belonging to the fields of rice high-yield stress-tolerant breeding and molecular genetics. By utilizing Jijing 88 and 3 donor-derived breeding population selection, a novel segregation distortion locating process suitable for breeding population selection is adopted to locate drought-tolerance-related QTL (quantitative trait loci); and random population selection is utilized to verify the authenticity of the drought-tolerant QTL and the reliability of the close linkage marker. When being applied to auxiliary selective breeding and polymerization breeding of drought-tolerant rice, the gene can effectively overcome the defects in the past spontaneous mutant genes, can obviously lower the negative influence on the auxiliary breeding due to the development of the initial locating result, and can carry out genotype selection on the lower-generation breeding populations in the seedling stage to obtain the drought-tolerance individuals, thereby facilitating the timely hybrid transformation, omitting the drought resistance identification process in the adult-plant stage, enhancing the breeding efficiency and accelerating the breeding progress.

Description

technical field [0001] The invention relates to a method of significantly increasing drought tolerance gene QDTY 2.9 in rice reproductive stage IR66897B and its molecular marker method, the gene significantly reduces the yield loss of rice under drought stress, belongs to the field of rice high-yield stress-resistant breeding and molecular genetics, and is suitable for introducing the drought-tolerant gene QDTY 2.9 in the reproductive period in rice high-yield stress-resistant breeding IR66897B , and use molecular markers for assisted selection breeding of the gene. Background technique [0002] Drought is one of the most important abiotic stress factors that cause rice yield loss and limit the distribution of rice planting. Currently, droughts occur frequently, causing huge losses to the world's rice production every year. However, the world's population is growing rapidly, and there is a huge gap in food demand. Therefore, it is a very urgent and major scientific issue t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12Q1/68C12N15/11
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 徐建龙黎志康朱亚军陈凯孟丽君崔彦茹
Owner AGRI GENOME INST OF SHENZHEN CHINESE ACADEMY OF AGRI SCI
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