678 results about "Japonica" patented technology

The invention relates to a method for preparing plant extract from Chinese traditional medicine honeysuckle (Lonicera japonica Thunb.) or honeysuckle or other plants of same congenic strains, the honeysuckle extract and medical compound containing said extract. The effective component of said compound is secologanic acid and its derivants. The method comprises following steps: extracting with water or alcohol, depositing extract, refining through chromatogram, purifying with chromatogram if needed, getting extract with secologanic acid and its derivants concentration being more than 50%, or about 70% if better. The honeysuckle extract is characterized by analgesic, analgesic, analgesic, anti-biotic and antiviral function, and can be used to treat analgesic, pain, infectious disease, especially to treat atypical pneumonia, viral influenza and AIDS.

The invention discloses a traditional Chinese medicine pesticide and belongs to the technical field of pesticides. The traditional Chinese medicine pesticide is prepared from the following crude drugs in parts by weight: 9-11 parts of anemarrhena asphodeloides, 9-11 parts of gardenia jasminoides, 9-11 parts of forsythia suspensa, 9-11 parts of radix stemonae, 4-6 parts of lonicera japonica, 2-4 parts of sophora flavescens, 2-4 parts of giant typhonium rhizome, 2-4 parts of illicium verum, 2-4 parts of common carpesium fruit, 2-4 parts of ulmus macrocarpa hance, 4-6 parts of rhizoma cimicifugae, 2-4 parts of folium artemisiae argyi, 4-6 parts of radix adenophorae, 2-4 parts of radix lithospermi, 4-6 parts of bunge corydalis herb, 2-4 parts of acorus calamus, 4-6 parts of garden balsam stem and 4-6 parts of radix isatidis. The invention also discloses a preparation method of the traditional Chinese medicine pesticide and an application of the traditional Chinese medicine pesticide to prevention of crop aphids, mites, viral diseases and cabbage worms. The crude drugs of the traditional Chinese medicine pesticide have different pharmacological characteristics; good insect killing, insect expelling and sterilization effects are achieved after the compatibility of the crude drugs; diseases can be prevented and cured; the application range is quite wide.

The invention relates to plant wound liquid containing chitosan oligosaccharide, which is characterized by comprising a plant growth regulator, wherein the regulator is one or more of naphthalene acetic acid and humic acid and accounts for 1 to 50 percent of the total weight of the liquid; and the chitosan oligosaccharide accounts for 0.0015 weight percent to 0.03 weight percent of the total weight of the liquid. The liquid can be prepared into any liquid preparation for spraying. One to five days before plants are trimmed, grafted or girdled, the liquid is used for spraying a wound. The liquid is used for apple trees, pear trees, peach trees, jujube trees, grape vines, mango trees, longan trees, lychee trees, mandarin trees, watermelons, gingko trees, lonicera japonica, jasmine, Chinese rose and the like.

A preparation method of traditional Chinese medicine lotion for treating carrion type cellulitis belongs to the technical field of traditional Chinese medicine preparation methods. Currently, antibiotic medicaments are generally used for the treatment of carrion type cellulitis, and have the disadvantage of great side effect. The technical scheme of the invention is that the preparation method comprises the following steps: soaking the following 37 herbs of herba violae, honeysuckle flower, fig, Chinese soapberry, semen calonyctionis muricati, malt culms, dandelion, dysosma versipellis, wikstroemia indica, herba saururi, radix berberidis, folium isatidis, big garden euphorbia herb, big herba lysimachiae, rhodea japonica root, aster ageratoides, subprostrate sophora, rhizoma pleionis, groundsel, lycopodium serratum, stephania, small garden euphorbia herb, meadowrue root and rhizome, purslane, leaf of Chinese coriaria, coptis, water pepper, sugarcane, cotton rose, radix sophorae flavescentis, bitter gourd, folium llicis latifoliae, elephantopus scaber, gentrin knotweed, saxifrage, radix scutellariae and licorice in water, decocting with mild fire, filtering to remove residues so as to obtain the medicinal liquid which is the traditional Chinese medicine lotion for treating carrion type cellulitis. The advantages are that the prepared traditional Chinese medicine is less in toxicand side effect, short in treatment course, and high in cure rate.

The invention discloses a bryophyte reversal-resistant protein PpLEA3-20 and an encoding gene and an application thereof. A plant resistance-related protein provided by the invention is named PpLEA3-20, is derived from physcomitrellapatens, and is a protein shown as (1) or (2), wherein (1) is constituted by an amino acid sequence shown as a sequence 2 in a sequence table, or (2) is obtained by performing substitution and/or deletion and/or addition of one or more amino acid residues on the amino acid residue sequence shown as the sequence 2 in the sequence table, is relevant to plant resistance and is derived from (1). As proved by an experiment for introducing the encoding gene of PpLEA3-20 into oryza sativassp.japonica, the survival rates (44 percent, 36 percent, 60 percent and 72 percent) and growth states of four obtained T2-generation PpLEA3-20 transgenic plants (20-1, 20-2, 20-3 and 20-4) in a drought stress experiment are remarkably better than those of a check plant.

The invention provides a method for preparing a formula feed for broiler chicken in the early stage. The formula feed is prepared from the following raw materials in parts by weight: 50-58 percent of corn, 8-12 percent of bran, 25-28 percent of soybean meal, 1-3 percent of DDGS, 0.5-1 percent of plant oil, 1-1.5 percent of shell powder, 0.3-0.6 percent of edible salt and 4-6 percent of mixed batch, wherein the mixed batch comprises astragalus membranaceus, lonicera japonica, hypericum monogynum, coptis chinensis, fructus forsythiae, radix isatidis, cyrtomium fortunei, pine needle meal, codonopsis pilosula and licorice root. The method comprises the following steps of conducting superfine grinding on the traditional Chinese medicinal materials, and adding an ethanol solution to the mixture; boiling the mixture with strong fire, conducting insulation with slow fire, conducting cooling and filtering, and concentrating the filtrate; adding white granulated sugar, and conducting boiling, cooling the mixture to a normal temperature, and inoculating lactobacillus, tea fungus and bifidobacterium; conducting fermentation for 96-120 hours until white fungal mass grows out, concentrating and drying the bacterial liquid at a low temperature, conducting embedding, and conducting granulation and cooling at a high temperature to prepare a granular feed which is the formula feed for broiler chicken in the early stage. The formula feed can be used for supplying amino acids and trace elements for growth of broiler chicken, has the effects of increasing the number of effective microbial communities in intestinal tracts, improving immunity, enhancing disease resistance of chicken bodies and promoting healthy growth of broiler chicken, and has the advantages of high content of viable organisms and no residue.

The invention discloses a method for processing lonicera japonica tea by reducing loss of chlorogenic acid. The concrete scheme comprises: directly processing or storing in a controlled atmosphere cold storage at the temperature range of 0 DEG C - 5 DEG C after plucking, then directly and quickly removing green with a high temperature steam, wherein the steam temperature range is 90 DEG C - 100 DEG C, the green removing time range is 88 - 92 seconds; then quickly cooling with cold air, wherein the cold air temperature is 3 DEG C - 7 DEG C, and the time for cooling is for 88 - 92 seconds; rapidly drying with high temperature and hot air, wherein the hot air temperature is 90 DEG C - 110 DEG C, and the time for drying is for 40 - 45 minutes; finally cooling and sealing. Therefore, before polyphenoloxidase is failed to decompose chlorogenic acid, the activity is destroyed due to high temperature and dehydration, then a rapid cryogenic cooling and drying with a high temperature are performed, so that a temperature range of effective activity can be avoided to converge the activity of polyphenoloxidase, thereby the effect of reducing loss of chlorogenic acid can be achieved as well as the product quality can be substantially improved.

The invention relates to a method for preparing short necked clam concentrated solution and a technical process thereof. The preparing technical process comprises the steps as follows: step 1: short necked clams spit sands and is cleaned; step 2: the short necked clams are selected and washed; step 3: the short necked clams are steamed and boiled into cooking liquor; step 4: the cooking liquor is filtered; step 5: the cooking liquor is sterilized under a high temperature; step 6: the cooking liquor is filtered again; step 7: the cooking liquor is concentrated in vacuum; and step 8: the cooking liquor is canned. The method for preparing the short necked clam concentrated solution and the technical process thereof have simple technology, and the produced short necked clam concentrated solution has fresh and delicious taste and abundant nutrition and long shelf life.

The invention relates to a DNA (deoxyribonucleic acid)-barcode-based eel species identification method, in particular to a DNA-barcode-based species identification method for anguilla japonica, anguilla rostrata and auguilla anguilla. The method comprises the following steps of performing eel DNA extraction, performing PCR (polymerase chain reaction) amplification to obtain a 244bp product by utilizing DNA barcode primers, and performing species identification according to nucleotide sequence characteristic variation sites of the species, wherein the DNA barcode primers are EEL244-F:CCTGTTCC TCGTGGGGGC TTTT and EEL244-R:ATGGCATAACGAGGGTTTAACTG. The method is low in time consumption, simple and convenient to operate and high in specificity, and can be used for identifying common processed eel products such as roasted eel which cannot be morphologically identified, and technical support is provided for the maintenance of enterprises and consumer benefits and normal market order.

The invention relates to the field of traditional Chinese medicine, and discloses a repair traditional Chinese medicine used for treating blood cell infected by flaviviridae. The traditional Chinese medicine is prepared from: by weight, herba tubocapsici anomali 30g-80g, cymbopogon citratus 20g-50g, camellia flower 10g-30g, citrus aurantium flower 10g-30g, bitter gourd 20g-50g, mint 20g-50g, phellodendron amurense 10g-30g, cypress leaf 10g-30g, salix leaf 10g-30g, persimmon leaf 15g-35g, perilla nankinensis 10g-20g, radix sophorae flavescentis 30g-60g, eleusine indica 25g-80g, syzygium aromaticum 10g-30g, lonicera japonica 30g-80g, agastache rugosus 20g-50g, portulaca oleracea 10g-30g, glycyrrhiza 5g-15g, chrysanthemum 10g-35g, and foeniculum vulgare 10g-35g. The traditional Chinese medicine has cure effect of the diseases such as dengue, Zika virus, encephalitis, hepatitis and liver cancer infected by flaviviridae spreading, can treat and repair the blood cell from the source, has prophylaxis and treatment effect on flaviviridae diseases, is without any toxicity and side effects, and can improve the cure rate and cure efficiency of patients.

The invention relates to a method for cultivating walnut shell powder bag material healthcare needle mushrooms. A cultivation medium is prepared by, by weight, 30-40 parts of bean stalk crumbs, 20-30 parts of rape stalk crumbs, 5-10 parts of bean meal, 6-8 parts of corn flour, 10-12 parts of bagasse, 1-2 parts of cicada powder, 1-2 parts of ginseng flower powder, 1-2 parts of polygala root powder, 2-3 parts of cortex albiziae powder, 2-3 parts of cherry stone powder, 1-2 parts of lxeris japonica powder, 2-3 parts of quicklime, 15-18 parts of bone soup, 1-2 parts of gypsum powder, 1-2 parts of walnut shell power, 0.5-1.0 part of silkworm excrement and 0.2-0.3 part of monopotassium phosphate. The method includes mixing the components according to a proportion, adding water, well mixing, bagging, sterilizing, grafting, producing bacteria in a bacterium producing chamber, replenishing nutrient solution and fruiting mushrooms. The needle mushroom cultivated by the method is nutrient-rich and high in content of trace elements, vitamins and calcium and has a walnut shell powder bag material healthcare function, anti-infection capability is high during needle mushroom fruiting, and needle mushroom yield is high.

A breeding method of a polyploid rice two-line restorer line includes determining hybrid parents for breeding the restorer line; breeding a restorer line of an indica sterile/japonica restorer type, or breeding a restorer line of a japonica sterile/indica restorer type, and carrying out backcrossing or composite hybridization after hybridization of the parents; selecting a single plant that meets the breeding goal, carrying out composite hybridization again and carrying out preliminary molecular marker detection; comparing, and selecting a single plant with good traits for continuous selfing until the line is basically stable; selecting a stable line with good traits and detection molecular markers; carrying out test-crossing on the preferred line as a male parent with different types of polyploid sterile lines; and selecting a good hybrid combination and a restorer line thereof.

The invention discloses a traditional Chinese medicament composition, which consists of the following components in parts by weight: 50 to 100 parts of rhizoma atractylodis macrocephalae, 50 to 150 parts of radix scutellariae, 20 to 40 parts of sophora flavescens, 60 to 150 parts of patchouli, 60 to 120 parts of astragalus, 60 to 100 parts of Chinese pulsatilla root, 25 to 60 parts of gardenia and 60 to 140 parts of lonicera japonica. The traditional Chinese medicament composition has reasonable proportion, and the effects of strengthening body resistance and eliminating evil, invigorating the spleen and eliminating dampness and clearing heat and stopping dysentery. The invention further discloses a preparation containing the traditional Chinese medicament composition, and the preparation is convenient to use and simple to prepare, and can be used for treating moist heat dysentery of swine, transmissible gastroenteritis of swine, weakened immune system and other diseases, thereby having a good application prospect.

The invention relates to a breeding method for an improved variety of Lonicera japonica Thunb No.1, and is applicable to breeding of new variety of Lonicera japonica Thunb. Farmyard germplasm type Lonicera japonica Thunb branches from main producing area of pingyi county, ShanDong province, are collected; 15-25 Gy60Co-Gamma is taken for radiation induced mutation, and then cuttage and transplant are carried out; seed selection, cottage and transplant for variation individual plant are performed so as to form a strain; the Lonicera japonica Thunb No.1 obtained through variety comparison and production testing has thick and upright branches, oblong, linear oblong and oblong-ovate leaves, has dark green and thick leaves, and has more blossom buds; and flower buds are 4.5 cm in length, bracts are oval and oblong-ovate, the flower season comes earlier, saline-alkaline tolerance and barren resistance and strong adaptability are achieved, and plant diseases and insect pests are less. Compared with common Lonicera japonica Thunb, the Lonicera japonica Thunb No.1 has the advantages that the flower season starts two to three days earlier, the flower bud yield is improved by 16.20 percent, the content of galuteolin of flower bud is 0.106 percent, and the content of chlorogenic acid accounts for 1.75 percent, which is higher than the quality standard of Pharmacopoeia of the People's Republic of China (2010).

The invention belongs to the technical field of traditional Chinese medicine and relates to a traditional Chinese medicine composite for treating haemorrhoids and the application thereof. The traditional Chinese medicine composite is prepared with portulaca oleracea, myrrh, panax pseudoginseng, mastix, rheum officinale, musk, dandelion, lonicera japonica, fresh rehmannia roots, honeysuckle stems, radix sanguisorbae, golden cypress, fructus forsythiae, pagoda tree fruits and liquorice. The traditional Chinese medicine composite is reasonable in medicine composition, remarkable effects of relieving swelling and pains, promoting blood circulation, removing blood stasis and clearing away heat and toxic materials in treating the haemorrhoids are achieved, the therapeutic effect of the composite is obvious, the medicine side effect is little, and the significant clinic promotion value is achieved.

The invention provides a molecular marking method for indica-japonica fragment substitution of fertility loci of an indica-japonica hybrid, which belongs to the field of molecular genetics. Molecular marker primers RMz 5, RMz7, RMz8 and RMz9 which are developed and designed by the invention amplify differential fragments at infertile loci of the indica-japonica hybrid, namely S5, S7, S8 and S9. Indica-japonica fragments can be substituted by molecular markers of the fertility gene loci; the required fragments can be precisely and quickly introduced; the selection efficiency of fragment substitution of the indica-japonica fertility loci is greatly improved, and a japonica type indica-compatible light and temperature-sensitive male sterile line 509S can be bred.

The invention provides meat goose feed and a preparation method thereof and belongs to the technical field of meat goose feed. The meat goose feed is composed of the following raw materials: corn, soybean meal, oat bran, wheat germ meal, expanding blood meal, bone meal, beer yeast powder, distillers' grains, alfalfa, Chinese prickly ash leaves, sweet potato leaves, fiveleaf gynostemma herb, silybum marianum, asparagus, ginseng leaves, pericarpium citri reticulatae, gingko, grifola frondosa, cattail pollen, Chinese wolfberry, longan, radix ophiopogonis, lonicera japonica, loaches, earthworms, table salt, monocalcium phosphate, compound enzyme, complex vitamins and compound trace elements. The preparation method for the feed comprises the following steps: 1), smashing and sterilizing; 2), culturing and pelletizing. The meat goose feed provided by the invention has the advantages that the weight of meat geese is increased quickly, the increase speed of lean meat of meat geese is increased, disgusting smell in the goose meat is reduced and the edible value of the meat geese is increased.

The invention introduces a production method of rice seeds. The production method is characterized by adopting rice variety seeds generated by intermediate indica japonica restorer R900. The intermediate indica and japonica restorer R900 is a high-yielding, high-quality and multi-resistant uniform new rice variety selected by breeding an intermediate material with big warehouse, sufficient sources, high setting percentage and good circularity by utilizing strong hybrid vigour of indica japonica subspecies through broad spectrum broad affinity genes, then selecting multi-resistant seeding head germplasm with polymerized indica japonica stress resistance genes by utilizing high pressure screening and finally a variety of rice which is high in yield, good in quality and uniform in multi-resistant performances can be obtained by inspecting the rice quality.

The invention discloses a Chinese medicinal preparation for treating cold stuffy nasal obstruction. The Chinese medicinal preparation is prepared from the following raw material medicaments in parts by weight: 3-15 parts of lonicera japonica, 3-15 parts of weeping forsythiae capsule, 5-10 parts of houttuynia cordata, 3-5 parts of ephedra herb, 2-6 parts of ginger, 8-12 parts of burdock, 5-15 parts of divaricate saposhnikovia root, 1-3 parts of Cherokee rose fruit, 1-3 parts of illicium verum, 3-8 parts of azalea, 10-40 parts of mung beans, and 2-4 parts of tuber onion seed. Through clinical application and observation, the Chinese medicinal preparation for treating the cold stuffy nasal obstruction has the advantages of quick response, good curative effective, complete cure and no toxic and side effects.

The invention belongs to the technical field of molecular mark assisted breeding, and particularly discloses a method for detecting genetic differentiation level of indica-japonica hybridizing parents and predicting the percentage of fructification of hybrid F1 by using InDel molecular markers. The hybridizing parents can be purposefully selected and the hybrid F1 with high percentage of fructification is prepared by detecting the genetic differentiation level of the indica-japonica hybridizing parents. The indica-japonica genetic differentiation coefficients (GDI) of indica-japonica hybridizing parents are determined by using 40 InDel molecular markers and a built calculation method, the correlation between the indica-japonica hybridizing parents GDI and a really measured mean value of the percentage of fructification (PF) of the hybrid F1 is really analyzed, so as to form a method for analyzing parents GDI to predict the percentage of fructification of the indica-japonica hybrid F1 by the molecular markers. The indica-japonica genetic differentiation coefficient detection on the parents can be carried out before hybridization, so as to predict the percentage of fructification of the hybrid F1. Thus, the indica-japonica hybridizing parents can be effectively selected.

The invention relates to Propylaea Japonica specific COI primers and a detection kit containing the primers. According to a unique mtDNA sequence of Propylaea Japonica, a pair of Propylaea Japonica specific COI primers, including PjF1 and PjR1 (SEQ ID No. 1 and 2), is designed, the pair of primers just has a specific amplification ability to Propylaea Japonica, the size of an amplification product is 256bp, the concentration detection limit of plasmids is 1.205943134 E+04, and the concentration detection limit of DNA is 0.00585938ng/mu L. According to the invention, a PCR technology is adopted, so that the detection accuracy is improved, and the detection time is saved, therefore, the Propylaea Japonica specific COI primers and detection kit containing the primers disclosed by the invention are suitable for popularization and application at the local level.