51 results about "Flavonols" patented technology

The invention discloses a method for extracting eucommia ulmoides with an intestinal repair function, and relates to the technical field of natural product extraction.The method comprises the steps that a fresh eucommia ulmoides raw material is subjected to low-temperature drying and smashing, a polysaccharide water extract and filter residues are obtained through water extraction, and then the flavone residual quantity in the polysaccharide water extract is synchronously detected; carrying out enzymolysis on the polysaccharide water extract through beta-glucanase to obtain a small molecular polysaccharide solution, and carrying out alcohol extraction on filter residues to obtain a flavone alcohol extract; the preparation method comprises the following steps: mixing the two components according to a ratio, inoculating compound probiotics for anaerobic fermentation, regulating according to the residual quantity of flavone, regulating an alcohol extraction process by using short-chain fatty acid generated by fermentation when the residual quantity is high, supplementing flavone extract in an enzymolysis system when the residual quantity is low, and finally removing impurities, purifying, adapting intestinal tracts and drying to obtain the product. According to the method, regulation and control operation is correspondingly executed by detecting the residual quantity of flavone in the polysaccharide water extract, so that self-supply of a regulation and control reagent and high efficiency of active ingredients are realized, the conversion rate of small molecular polysaccharide and flavone aglycone is increased, and the intestinal tract tolerance of the product is optimized.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a glycyrrhiza uralensis n-butyl alcohol extract and application of effective components thereof. The invention relates to a glycyrrhiza uralensis n-butyl alcohol extract. The effective components of the glycyrrhiza uralensis n-butyl alcohol extract are prepared from glycyrrhiza uralensis isoflavanone, glycyrrhiza stem alcohol A, licorcidine, dibutyl phthalate, glycyrrhizin I, 3 '-Dimethylallylkievitone, lupinone, glycyrrhiza flavonol, edediol, 1-methoxy phaseolin and isoderrone. The glycyrrhiza uralensis n-butyl alcohol extract is prepared from the following raw materials: glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis and glycyrrhiza uralensis, the anti-streptococcus mutans activity of the glycyrrhiza uralensis n-butyl alcohol extract provided by the invention is superior to that of an extract extracted by ethanol in the same extraction method. The method has more advantages in the aspect of preventing streptococcus mutans infection and decayed teeth, and a theoretical basis is provided for research and development of new products.

The present invention provides a protective film agent and a method for processing a workpiece, the protective film agent being capable of forming a protective film suitable for processing with a laser beam. The protective film agent is coated on a workpiece when the workpiece is processed, the protective film agent comprising: a water-soluble resin; a light absorber having a flavone structure, a flavonol structure, or an isoflavone structure; and a solvent that dissolves the resin and the light absorber.

The reagent for detecting MHC-II expression of tumor cells in gastric cancer tissue comprises the following components in parts by mass: 10-30 parts of paraffin section preparation liquid, 2-10 parts of dewaxing liquid, 2-10 parts of sodium citrate antigen repair liquid, and 20-40 parts of immunohistochemical staining liquid; the dewaxing liquid comprises dimethylbenzene, and at least one of flavonol aminobenzoate derivative and sinapamide benzyl trimethylammonium benzoate salt; the flavonol aminobenzoate derivative has a nitrogen-containing group and a benzene ring, and the sinapamide benzyl trimethylammonium benzoate salt has a long-chain unsaturated alkane and a nitrogen-containing group. The reagent for detecting MHC-II expression of tumor cells in gastric cancer tissue can obtain high-quality immunohistochemical staining sections, thereby improving the sensitivity and specificity of MHC-II expression detection.

The application relates to a method for indoor cultivation of medicinal mulberry and application thereof in improving secondary metabolites of indoor cultivated medicinal mulberry, which comprises transplanting, illumination, fertilization and management steps. The application effectively improves the secondary metabolites of indoor cultivated medicinal mulberry by applying inorganic fertilizer during the growth period of the medicinal mulberry. Especially, high-potassium fertilizer is most obvious for improving the active substances of indoor medicinal mulberry. The high-potassium fertilizer can improve the content of flavones and flavonols in the medicinal mulberry by affecting the biosynthetic metabolic pathways of the flavones and the flavonols.

The invention discloses a method for regulating and controlling synthesis of tobacco flavonol and anthocyanin, a target gene and application of the target gene, and aims to solve the technical problem that biosynthesis of anthocyanin and flavonol of a current plant is not easy to regulate and control. According to the invention, a gene AFB capable of regulating and controlling tobacco biosynthesis is cloned from tobacco, an overexpression vector and a CRISPR / Cas9 vector of the gene AFB are constructed, and corresponding overexpression strains and gene knockout plants are respectively obtained through transformation; phenotype and physiological index determination shows that AFB belongs to an HD-ZIP IV family member, can regulate and control development of corolla conical epidermis cells and biosynthesis of plant anthocyanin and flavonol so as to control flower color, and has important application value in the aspects of flower color regulation and control, construction or screening of high-resistance and high-quality plant varieties and the like.

The invention discloses a flavonol-based fluorescent probe for continuously detecting hydrogen sulfide and hypochlorous acid as well as a preparation method and application of the flavonol-based fluorescent probe. The flavonol-based fluorescent probe is 3-(2, 4-dinitrophenoxy)-7-(4-(diphenylamino) phenyl)-2-(4-(thiomorpholinophenyl) chromone, and the structural formula of the flavonol-based fluorescent probe is shown in the specification. According to the invention, 7-(4-(diphenylamino) phenyl)-3-hydroxy-2-(4-thiomorpholinophenyl) chromone is used as an initial raw material, and the 7-(4-(diphenylamino) phenyl)-3-hydroxy-2-(4-thiomorpholinophenyl) chromone and 2, 4-dinitrofluorobenzene are subjected to nucleophilic substitution reaction to obtain the compound 3-(2, 4-dinitrophenoxy)-7-(4-(diphenylamino) phenyl)-2-(4-(thiomorpholinophenyl) chromone. The compound is firstly subjected to selective thiolysis reaction under the action of hydrogen sulfide, the fluorescence color of a solution of the compound is changed from colorless to bright orange under the irradiation of ultraviolet light with the wavelength of 365 nm, then hypochlorous acid is continuously added into the solution, and the compound can be further subjected to specific oxidation reaction with the hypochlorous acid, so that the compound can be obtained. And the fluorescence color of the solution is changed from bright orange to bright cyan. Therefore, the compound can be used as a fluorescent probe for continuously detecting hydrogen sulfide and hypochlorous acid, has the advantages of low detection limit, high response speed, good light stability, wide pH application range and the like, and shows a good application prospect.

The application discloses application of a flavonoid metabolic pathway network key regulation gene GmCHR1 in Arabidopsis thaliana. Research shows that the expression amount of a soybean chalcone reductase (CHR) gene GmCHR1 in soybean roots is significantly up-regulated under low-phosphorus stress, and the low-phosphorus stress enhances the expression of GmCHR1 in the root system of Arabidopsis thaliana, and GmCHR1 influences the response of the root system of Arabidopsis thaliana to low-phosphorus stress. Through construction of a transgenic plant, overexpression of the GmCHR1 gene in Arabidopsis thaliana can promote the growth of Arabidopsis thaliana, significantly improve the biomass, total root length and total phosphorus content of the transgenic Arabidopsis thaliana under normal phosphorus conditions and low-phosphorus conditions; meanwhile, overexpression of GmCHR1 reduces the accumulation of flavonol in the root tip of the transgenic Arabidopsis thaliana, thereby enhancing the accumulation of auxin in the root tip of the transgenic Arabidopsis thaliana, positively regulating the synthesis of auxin in the root system of Arabidopsis thaliana and improving the content of auxin, and playing an important role in improving the growth and phosphorus efficiency of Arabidopsis thaliana.

The invention discloses a tea tree MYB transcription factor CsMYB330 gene and application, the nucleotide sequence of the MYB transcription factor CsMYB330 gene is shown as SEQ ID NO: 1, the expression of the gene participates in regulating the flavonol content in plants, and the instantaneous silencing of the CsMYB330 gene in the tea tree can significantly reduce the flavonol content in the tea tree by 24.5% and reduce the bitter taste of the finished tea.

This invention provides a composition of Ginkgo biloba extract and its preparation method. The composition consists of Ginkgo biloba extract (calculated as total flavonol glycosides) and dipyridamole in a dosage ratio of 10:4. The preparation method can significantly improve product yield, reduce production costs, and significantly increase the content of the main components of the produced Ginkgo biloba extract, namely total flavonol glycosides and terpene lactones. It can also reduce the content of poorly water-soluble ginkgolides and harmful impurities such as ginkgolic acid, control its microbial level, and improve the stability and safety of the Ginkgo biloba extract preparation product of this invention.

The application discloses a thiazole-containing flavonol derivative and a preparation method and application thereof, and belongs to the technical field of pesticide synthesis. A thiazole-containing flavonol derivative is generated by substitution reaction of 3-(bromoalkyloxy)-7-substituent-2-(4-substituent phenyl)-4H-chromone-4-ketone and 5-substituent-2-thiol-1,3,4-thiadiazole, and a structural formula is shown as formula Y: wherein n is 3 or 4; R1 is hydrogen or an amino group; R2 is hydrogen, an alkyl group or an alkoxy group; and R3 is hydrogen, a halogen, an alkyl group or an alkoxy group. The thiazole-containing flavonol derivative has good bacteriostatic activity and can be applied to the preparation of bacteriostatic medicaments.

The invention provides a composition of ginkgo biloba extract and a preparation method thereof, the composition is composed of ginkgo biloba extract (based on total flavonol glycosides) and dipyridamole according to a dosage ratio of 10: 4, and the preparation method can significantly improve the product yield, reduce the production cost and improve the product quality. The ginkgo biloba extract prepared by the method has the advantages that the content of main components, namely total flavonol glycosides and terpene lactones, of the ginkgo biloba extract is obviously increased, the content of bilobalide with poor water solubility and the content of a harmful impurity, namely ginkgolic acid can be reduced, the microbial level of the ginkgo biloba extract is controlled, and the stability and safety of the ginkgo biloba extract preparation product are improved.

The invention relates to a flavonol compound dysosmaflavonoids I as well as a preparation method and application thereof, and can effectively solve the problems of preparation of the flavonol compound and preparation of antitumor drugs, the flavonol compound is dysosmaflavonoids I extracted and separated from dysosmaflavonoids I, has a growth inhibition effect on human liver cancer cells HepG2 and human colon cancer cells SW480, and can be used for preparing antitumor drugs. And the method has good practical application value.

The present application belongs to the technical field of quality control of traditional Chinese medicine, and particularly relates to a quality control method of Ledum palustre L. The quality control method provided by the present application comprises the following processes: S1, identification: thin layer chromatography identification is respectively performed by taking ursolic acid, hyperoside, rutin, aesculetin and aesculin as the control; S2, inspection: the moisture content, total ash content, acid insoluble ash content and alcohol-soluble extract content of the medicinal material are determined; S3, content determination: the total content of volatile oil, three five-ring triterpenes (betulinic acid, oleanolic acid and ursolic acid) and two flavonoid glycosides (hyperoside and preussin) in the medicinal material is determined by using high performance liquid chromatography. The present application combines multi-index component analysis and thin layer chromatography identification, has strong specificity and good reproducibility, can comprehensively and effectively control the quality of Ledum palustre L., and ensures the medicinal effectiveness and safety of Ledum palustre L.

The invention relates to the technical field of coreopsis tinctoria refined components and preparation and application, and provides a coreopsis tinctoria refined component and a preparation method and application thereof.The coreopsis tinctoria refined component comprises, by mass, 11 specific components of phenolic acids, flavonol, flavanol and flavonoid, and the 11 specific components comprise, by mass, 2 parts of gallic acid, 1 part of potassium sorbate, 1 part of potassium sorbate, 1 part of potassium sorbate and 1 part of potassium sorbate. The health-care food is prepared from the following raw materials in parts by weight: 3 parts of chlorogenic acid, 14 parts of quercetin, 18 parts of catechin, 5 parts of citric acid, 2-3 parts of procyanidine B, 3 parts of (+)-epicatechin, 1-31 parts of 31 parts of procyanidine C, 14 parts of rutin, 4 parts of camphanol-3-O-rutinoside and 2 parts of isorhamnetin-3-O-rutinoside. The snow chrysanthemum is Changbai Mountain snow chrysanthemum. Animal experiments show that the refined component can significantly reduce the content of total cholesterol and triglyceride in serum of a high-fat model rat, the specific component with blood fat regulating activity is prepared from Changbai Mountain snow chrysanthemum, and a new choice is provided for non-drug intervention of dyslipidemia.

The application discloses a method for catalyzing sodium borohydride to produce hydrogen at low temperature, which is characterized by using natural flavonoids (including flavone, flavonol, isoflavone and the like) as a catalyst, using methanol or a mixture of methanol and water as a reaction solvent, using NaBH4 as a raw material for hydrogen production, and performing alcoholysis of NaBH4 to produce hydrogen. The catalyst used in the application is abundant in source and low in price, can catalyze sodium borohydride to produce hydrogen through alcoholysis at a wide reaction temperature range, has stable catalytic performance, high hydrogen production rate, good catalytic durability and reusability, and has important significance for the application and popularization of sodium borohydride in the field of hydrogen production.

This invention belongs to the field of pharmaceutical technology and relates to a preparation process of a modified Huoxiang Zhengqi compound. The preparation process is as follows: Pinellia ternata and Angelica dahurica are percolated; Pogostemon cablin, Perilla frutescens, Citrus reticulata peel, Magnolia officinalis, and Atractylodes macrocephala are extracted in groups for 2-5 hours, and the total volatile oil is collected. The residues of each group are decocted with water, and the decoctions of each group are collected; Poria cocos, Platycodon grandiflorus, Glycyrrhiza uralensis, Areca catechu peel, Ziziphus jujuba, and Zingiber officinale are decocted with water to obtain a decoction; the total volatile oil is added to an inclusion material, and inclusion is obtained using an ultrasonic-assisted method; the percolation and decoctions are combined, concentrated, and centrifuged to obtain a supernatant; a preservative is added to the supernatant and the inclusion material to obtain the final product. This invention extracts medicinal materials containing volatile oils in groups and uses a cyclodextrin inclusion complexation process, which not only improves the total extraction rate of volatile oils, but also increases the content of hesperidin, hydrated oxypenicillin, magnolol, honokiol, patchouli flavonol, and baicalein, while improving the taste and flavor of the flavored patchouli flavonoid compound.

To provide a polyphenol composition allowing specific polyphenols, being water-poorly-soluble compounds, to be dissolved at high concentration in a water-based solvent and enabling easy preparation of a highly transparent solution, and a method for producing the same.SOLUTION: The polyphenol composition of the present invention includes a water-poorly-soluble compound and a sucrose fatty acid ester, wherein the water-poorly-soluble compound is one polyphenol selected from the group consisting of flavones, flavanones, isoflavones, and flavonols, and the sucrose fatty acid ester has an average degree of esterification of 1.01 or more and 1.1 or less.SELECTED DRAWING: None

This application belongs to the field of food processing technology, and particularly relates to a preparation technology and application of fruit powder products with high citrus flavonoid content. The preparation technology of fruit powder products with high citrus flavonoid content provided by this application first extracts blood orange peel through supercritical CO2 combined with ethanol extraction. The obtained blood orange peel flavonoid extract contains rich polymethoxylated flavonoid active ingredients, flavanones, flavonols, and flavonoid active ingredients. After being added to fruit powder, the types of flavonoids and the total flavonoid content are greatly increased, and the antioxidant and other biological activities are improved, thereby solving the problem of the lack of deep processing technology for whole blood oranges in the prior art.

This invention relates to the field of traditional Chinese medicine extraction technology, specifically disclosing a method for ultrasonic extraction of flavonol glycosides from Epimedium using a eutectic solvent-assisted process. The method involves mixing choline chloride and glycerol, heating to prepare a eutectic solvent; mixing Epimedium leaf powder with a mixture of 70% ethanol and the eutectic solvent; ultrasonic extraction followed by centrifugation and filtration to obtain the Epimedium flavonol glycoside extract. The extraction method provided by this invention is green, efficient, and operates under mild conditions, resulting in good extraction effects. Compared with conventional ethanol ultrasonic extraction, the extracted icariin content is increased by 37.93%, and the total content of the three monomers is increased by 22.99%, providing technical support for the large-scale preparation and further development and utilization of Epimedium flavonol glycosides.

The application discloses a flavonol derivative containing piperazine-isopropyl alcohol amine and a preparation method and application thereof, and belongs to the technical field of pesticide synthesis. The application introduces piperazine-isopropyl alcohol amine with excellent activity into the structure of flavonol, and synthesizes a series of flavonol derivatives containing piperazine-isopropyl alcohol amine. Through activity test of the synthesized compounds on inhibition of plant pathogenic bacteria, it is found that the synthesized flavonol derivatives containing piperazine-isopropyl alcohol amine can effectively inhibit plant pathogenic bacteria, especially pepper phytophthora blight bacteria ( P.c ) and rice bacterial leaf blight bacteria ( Xoo ).

The invention discloses recombinant escherichia coli with high yield of cyanidin-3-O-glucoside as well as a construction method and application of the recombinant escherichia coli. The method comprises the following steps: by taking escherichia coli BL21 (DE3) engineering bacteria as a host, integrating flavanonol 4-reductase, anthocyanin synthetase and flavonoid 3-O-glucosyltransferase from different sources onto a plasmid vector pRSFDuet, and constructing to obtain a chassis cell for producing C3G; meanwhile, glutathione transferase is overexpressed, dissolution-promoting tag small ubiquitin-like modified protein is added, FaDFR is modified through enzyme engineering, FaDFR catalytic efficiency is improved through artificial protein cage space combination pathway enzyme, key precursor UDP-glucose is synthesized in an in-situ intensified mode and cofactor engineering, the cell factory for efficiently synthesizing C3G is constructed based on the combination strategy, the yield of C3G is increased, and the method has the advantages that the method is simple and convenient to operate, and the cost is low. The titer reaches 797.20 mg / L. The method provided by the invention has the characteristics of simplicity, economy and eco-friendliness.

This invention relates to compounds that are 4′-substituted analogues of the flavonol Fisetin. In particular, the invention relates to such compounds wherein the 4′ position on the B-ring is substituted with a ring deactivating group which has a para-Hammett constant greater than zero, and the use of these compounds in the treatment of cancer, including epithelial cancers.

This invention discloses a method for preparing dihydroisoflavone derivatives. Specifically, this invention provides a method for preparing compound 16, which includes the following steps: in an organic solvent, in the presence of an alkoxide, a catalyst, and a ligand, compound 12 and compound 15 undergo a Suzuki-Miyaura reaction to obtain compound 16; the catalyst is Pd(OAc)2; and the ligand is PPh3. This application designs and synthesizes the target natural product (compound 1) through a total synthesis method. This research provides a basis for the total synthesis of dihydroisoflavone alcohol compounds containing chiral centers.

The present application relates to the field of pesticides, in particular to a flavonol derivative and application thereof.The flavonol derivative of the present application is shown as (I), wherein R1 is selected from hydrogen, halogen, alkyl or alkoxy; R2 is selected from hydrogen, alkyl or alkoxy.The flavonol derivative of the present application has good bacteriostatic effect on rice sheath blight fungus, cucumber anthracnose fungus and strawberry botrytis cinerea, can be used for preventing and treating plant fungal diseases, and has low cost of raw material for synthesis and simple synthesis method.

The application discloses Fangcheng tea high-dihydroxyl catechin related molecular markers based on a liquid phase chip and application thereof, and belongs to the technical field of biology. The application identifies 17 SNP mutation sites distributed on flavonoid 3'5' hydroxylase, flavonoid 3' hydroxylase, leucoanthocyanidin reductase and dihydroxy flavonol reductase coding genes of Fangcheng tea by using a liquid phase chip and a large amount of transcriptome data developed in an early stage. The mutation sites can significantly affect the expression of related genes, thereby regulating the distribution ratio of dihydroxyl catechin and trihydroxyl catechin of Fangcheng tea. According to the genotypic characteristics, high-dihydroxyl catechin Fangcheng tea germplasm resources can be quickly screened and identified. The application fills the technical blank of accurately, efficiently and low-costly identifying high-dihydroxyl Fangcheng tea resources, and also provides a theoretical basis for researching high-dihydroxyl catechin molecular mechanisms.

The application discloses a neutral bright tin plating solution and a preparation method and application thereof. The plating solution is composed of tin salt, tin complexing agent, brightener, dispersant and pH buffer. The molar concentration of the tin salt is 0.10-0.40 mol / L. The tin complexing agent is mercaptoacetic acid and its salt. The brightener is flavonol substance. The dispersant is nonylphenol polyoxyethylene ether substance. The pH of the neutral bright tin plating solution is 6.0-8.0. The plating solution has high stability and service life, small hydrogen evolution phenomenon, simple components and easy management. The synergistic effect among the components improves the dispersion capacity and covering capacity of the plating solution, thereby effectively ensuring the performance of the tin-plated parts prepared by plating, and the tin-plated parts have good combination, low porosity, uniformity, compactness and bright appearance, and are beneficial to application in electronic components or mechanical manufacturing technology.

The invention belongs to the technical field of extraction and separation, and particularly relates to a preparation method and application of rhizoma polygonati total flavonoids. According to the preparation method provided by the invention, the extraction rate of the total flavonoids of the rhizoma polygonati can be increased, 324 flavonoid components are analyzed and identified in the extracted flavonoid extract and mainly contain flavones, flavanone, flavonol, isoflavone and other flavonoids, the content of effective components is high, the preparation process is simple and controllable, and the method is suitable for large-scale production; the total flavonoids of polygonatum sibiricum provided by the invention can effectively relieve oxidative stress of HK-2 cells induced by uric acid and improve the oxidation resistance of the cells, so that the toxic effect of a hyperuricemia environment on kidney cells is relieved; and further research shows that by adjusting the expression of a kidney uric acid transporter URAT1, uric acid reabsorption can be reduced, kidney uric acid excretion can be promoted, and the compound has a good application prospect in preparation of uric acid lowering drugs, anti-hyperuricemia drugs and the like.

The application discloses a benzothiazole-containing flavonol derivative, characterized by a structural formula as shown in the formula L, wherein when R1 is not a hydrogen atom, R1 is a methyl group, a methoxy group, a tert-butyl group or a halogen atom at an ortho position, a para position or a meta position, and R2 is a methyl group, a methoxy group or a halogen atom. The benzothiazole-containing flavonol derivative is subjected to an anti-virus activity test, and it is proved that the benzothiazole-containing flavonol derivative has good anti-virus activity and can be applied to the preparation of a plant virus inhibiting agent.