75 results about "Flavonols" patented technology

The invention discloses a carrier-free nano-particle with pH / ROS dual response as well as a preparation method and application of the carrier-free nano-particle. The preparation method comprises the following steps: enabling quinolizidine alkaloid and flavonol to be subjected to self-assembly, so as to prepare quinolizidine alkaloid-flavonol carrier-free nano particles; modifying the quinolizidine alkaloid-flavonol carrier-free nano particles by adopting a phenylboronic acid-hyaluronic acid conjugate, so as to prepare nano particles modified by the phenylboronic acid-hyaluronic acid conjugate; and coating the surfaces of the nano-particles modified by the phenylboronic acid-hyaluronic acid conjugate with a pH sensitive polymer to prepare the pH / ROS dual-response carrier-free nano-particles. The sophocarpidine-kaempferol carrier-free nanoparticles with pH / ROS dual response, prepared by the invention, have the advantages of good targeting property, high biocompatibility, small toxicity and adverse reaction and the like, meanwhile, the nanoparticles have a good anti-inflammatory effect, and a new choice is provided for the treatment of ulcerative colitis.

The invention discloses a method for extracting eucommia ulmoides with an intestinal repair function, and relates to the technical field of natural product extraction.The method comprises the steps that a fresh eucommia ulmoides raw material is subjected to low-temperature drying and smashing, a polysaccharide water extract and filter residues are obtained through water extraction, and then the flavone residual quantity in the polysaccharide water extract is synchronously detected; carrying out enzymolysis on the polysaccharide water extract through beta-glucanase to obtain a small molecular polysaccharide solution, and carrying out alcohol extraction on filter residues to obtain a flavone alcohol extract; the preparation method comprises the following steps: mixing the two components according to a ratio, inoculating compound probiotics for anaerobic fermentation, regulating according to the residual quantity of flavone, regulating an alcohol extraction process by using short-chain fatty acid generated by fermentation when the residual quantity is high, supplementing flavone extract in an enzymolysis system when the residual quantity is low, and finally removing impurities, purifying, adapting intestinal tracts and drying to obtain the product. According to the method, regulation and control operation is correspondingly executed by detecting the residual quantity of flavone in the polysaccharide water extract, so that self-supply of a regulation and control reagent and high efficiency of active ingredients are realized, the conversion rate of small molecular polysaccharide and flavone aglycone is increased, and the intestinal tract tolerance of the product is optimized.

The invention discloses cloning and functional identification of a liverwort flavone synthetase gene, and belongs to the technical field of gene engineering. According to the invention, one flavone synthase I (flavones synthase I, FNS I) is obtained through screening, and is named as MpFNS I; a prokaryotic expression system is utilized, an MpFNS I recombinant protein is obtained through purification, and the result of in-vitro enzyme activity identification shows that an MpFNS I enzyme substrate is relatively wide in selectivity, has a flavone synthetase function and also has a part of flavonol synthetase function. The liverwort flavonoid has a significant difference on affinity and catalytic efficiency of different substrates, the in-vivo function of the liverwort flavonoid is verified by utilizing heterologous expression of arabidopsis thaliana, experimental evidence is provided for comprehensively analyzing a biosynthetic pathway of the liverwort flavonoid, and meanwhile, a research target is provided for deeply researching a mechanism for synthesizing and responding to UV-B radiation of the liverwort flavonoid.

The present invention discloses the preparation of patchouli flavonoid nanoliposomes and their application in inhalation treatment of acute lung injury, and relates to the field of medical technology. The patchouli flavonoid nanoliposomes provided by the present invention prepare patchouli flavonoids into a liposome dosage form, which has good stability and biocompatibility. The drug is administered to mice with acute lung injury, so that patchouli flavonoids can act on the mouse lungs in a targeted manner, reduce the expression of inflammatory factors such as IL-6, IL-1β and TNF-α in the mouse lungs to relieve inflammation, improve pulmonary edema, and reduce lung tissue damage, while increasing the levels of tight junction factors such as ZO-1, VE-Cadherin, E-Cadherin and Claudin5 to repair lung barrier damage. The present invention is expected to provide an effective strategy for the treatment of acute lung injury.

The invention discloses a detection reagent for detecting MHC-II expression of gastric cancer tissue tumor cells. The detection reagent comprises the following components in parts by mass: 10-30 parts of a paraffin section preparation solution, 2-10 parts of a dewaxing solution, 2-10 parts of a sodium citrate antigen repair solution and 20-40 parts of an immunohistochemical staining solution, the dewaxing liquid comprises xylene and at least one of a flavonol aminobenzoate derivative and erucyl amide benzoic acid benzyl trimethyl ammonium salt, the flavonol aminobenzoate derivative has a nitrogen-containing group and a benzene ring, and the erucyl amide benzoic acid benzyl trimethyl ammonium salt has long-chain unsaturated alkane and a nitrogen-containing group. The invention provides a detection reagent for detecting MHC-II expression of gastric cancer tissue tumor cells, and high-quality immunohistochemical staining sections can be obtained, so that the sensitivity and specificity of MHC-II expression detection are improved.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a glycyrrhiza uralensis n-butyl alcohol extract and application of effective components thereof. The invention relates to a glycyrrhiza uralensis n-butyl alcohol extract. The effective components of the glycyrrhiza uralensis n-butyl alcohol extract are prepared from glycyrrhiza uralensis isoflavanone, glycyrrhiza stem alcohol A, licorcidine, dibutyl phthalate, glycyrrhizin I, 3 '-Dimethylallylkievitone, lupinone, glycyrrhiza flavonol, edediol, 1-methoxy phaseolin and isoderrone. The glycyrrhiza uralensis n-butyl alcohol extract is prepared from the following raw materials: glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis and glycyrrhiza uralensis, the anti-streptococcus mutans activity of the glycyrrhiza uralensis n-butyl alcohol extract provided by the invention is superior to that of an extract extracted by ethanol in the same extraction method. The method has more advantages in the aspect of preventing streptococcus mutans infection and decayed teeth, and a theoretical basis is provided for research and development of new products.

The present invention provides a protective film agent and a method for processing a workpiece, the protective film agent being capable of forming a protective film suitable for processing with a laser beam. The protective film agent is coated on a workpiece when the workpiece is processed, the protective film agent comprising: a water-soluble resin; a light absorber having a flavone structure, a flavonol structure, or an isoflavone structure; and a solvent that dissolves the resin and the light absorber.

The reagent for detecting MHC-II expression of tumor cells in gastric cancer tissue comprises the following components in parts by mass: 10-30 parts of paraffin section preparation liquid, 2-10 parts of dewaxing liquid, 2-10 parts of sodium citrate antigen repair liquid, and 20-40 parts of immunohistochemical staining liquid; the dewaxing liquid comprises dimethylbenzene, and at least one of flavonol aminobenzoate derivative and sinapamide benzyl trimethylammonium benzoate salt; the flavonol aminobenzoate derivative has a nitrogen-containing group and a benzene ring, and the sinapamide benzyl trimethylammonium benzoate salt has a long-chain unsaturated alkane and a nitrogen-containing group. The reagent for detecting MHC-II expression of tumor cells in gastric cancer tissue can obtain high-quality immunohistochemical staining sections, thereby improving the sensitivity and specificity of MHC-II expression detection.

The application relates to a method for indoor cultivation of medicinal mulberry and application thereof in improving secondary metabolites of indoor cultivated medicinal mulberry, which comprises transplanting, illumination, fertilization and management steps. The application effectively improves the secondary metabolites of indoor cultivated medicinal mulberry by applying inorganic fertilizer during the growth period of the medicinal mulberry. Especially, high-potassium fertilizer is most obvious for improving the active substances of indoor medicinal mulberry. The high-potassium fertilizer can improve the content of flavones and flavonols in the medicinal mulberry by affecting the biosynthetic metabolic pathways of the flavones and the flavonols.

The invention discloses a method for regulating and controlling synthesis of tobacco flavonol and anthocyanin, a target gene and application of the target gene, and aims to solve the technical problem that biosynthesis of anthocyanin and flavonol of a current plant is not easy to regulate and control. According to the invention, a gene AFB capable of regulating and controlling tobacco biosynthesis is cloned from tobacco, an overexpression vector and a CRISPR / Cas9 vector of the gene AFB are constructed, and corresponding overexpression strains and gene knockout plants are respectively obtained through transformation; phenotype and physiological index determination shows that AFB belongs to an HD-ZIP IV family member, can regulate and control development of corolla conical epidermis cells and biosynthesis of plant anthocyanin and flavonol so as to control flower color, and has important application value in the aspects of flower color regulation and control, construction or screening of high-resistance and high-quality plant varieties and the like.

The invention discloses a flavonol-based fluorescent probe for continuously detecting hydrogen sulfide and hypochlorous acid as well as a preparation method and application of the flavonol-based fluorescent probe. The flavonol-based fluorescent probe is 3-(2, 4-dinitrophenoxy)-7-(4-(diphenylamino) phenyl)-2-(4-(thiomorpholinophenyl) chromone, and the structural formula of the flavonol-based fluorescent probe is shown in the specification. According to the invention, 7-(4-(diphenylamino) phenyl)-3-hydroxy-2-(4-thiomorpholinophenyl) chromone is used as an initial raw material, and the 7-(4-(diphenylamino) phenyl)-3-hydroxy-2-(4-thiomorpholinophenyl) chromone and 2, 4-dinitrofluorobenzene are subjected to nucleophilic substitution reaction to obtain the compound 3-(2, 4-dinitrophenoxy)-7-(4-(diphenylamino) phenyl)-2-(4-(thiomorpholinophenyl) chromone. The compound is firstly subjected to selective thiolysis reaction under the action of hydrogen sulfide, the fluorescence color of a solution of the compound is changed from colorless to bright orange under the irradiation of ultraviolet light with the wavelength of 365 nm, then hypochlorous acid is continuously added into the solution, and the compound can be further subjected to specific oxidation reaction with the hypochlorous acid, so that the compound can be obtained. And the fluorescence color of the solution is changed from bright orange to bright cyan. Therefore, the compound can be used as a fluorescent probe for continuously detecting hydrogen sulfide and hypochlorous acid, has the advantages of low detection limit, high response speed, good light stability, wide pH application range and the like, and shows a good application prospect.

Provided are a pharmaceutical composition for preventing, ameliorating or treating atopic diseases comprising a flavonoid derivative as an active ingredient, and a method for preparing the flavonoid derivative, and more specifically, provided are a pharmaceutical composition for preventing, ameliorating or treating atopic diseases comprising a flavonoid derivative 3, 3 ', 3', 3 ', 3', 3 ', 3', 3 ', 3', 3 ', 3', 3 ', 3', 3 ', 3', 3 ' The invention relates to a pharmaceutical composition containing 3, 4-dihydroxyflavonol as an active ingredient and a method for preparing the 3, 4-dihydroxyflavonol.

The invention discloses application of a QUE analogue in preparation of a medicine for relieving acute lung injury caused by bacterial LPS, and belongs to the technical field of biological medicine. The preparation method of the QUE analogue comprises the following steps: dissolving 5-bromo-2, 4-dihydroxybenzoic acid in an organic solvent, then adding EDC, flavonol and DMAP, and purifying after low-temperature reaction to obtain the QUE analogue. The QUE analogue (namely Q8) prepared by the invention can be used for reducing inflammatory response and lung pathological injury caused by bacterial LPS (Lipopolysaccharide).

The present invention relates to the field of traditional Chinese medicine processing technology. The present invention quantifies the three factors of frying temperature, frying time, and auxiliary material dosage, establishes an objective evaluation method, optimizes the processing technology of honey mulberry leaves using the Box-Behnken design-response surface method, detects the index components of honey mulberry leaves using modern instrumental analysis methods, calculates the weight coefficients of seven indicators including chlorogenic acid, rutin, isoquercetin, astragalin, total flavonoids, alcohol extract, and moisture using the CRITIC-AHP composite weighting method, and calculates the overall evaluation "normalization value" to obtain the optimal processing technology results. The final optimized processing process steps are as follows: take 7.5g of refined honey, dilute it with boiling water (mulberry leaf: boiling water = 1:2), pour it into 30g of clean mulberry leaf medicinal materials, mix well, simmer for 4 minutes, place it in a hot pot, fry it at 170°C for 10 minutes, remove it and cool it, and then seal it for storage.

The application discloses application of a flavonoid metabolic pathway network key regulation gene GmCHR1 in Arabidopsis thaliana. Research shows that the expression amount of a soybean chalcone reductase (CHR) gene GmCHR1 in soybean roots is significantly up-regulated under low-phosphorus stress, and the low-phosphorus stress enhances the expression of GmCHR1 in the root system of Arabidopsis thaliana, and GmCHR1 influences the response of the root system of Arabidopsis thaliana to low-phosphorus stress. Through construction of a transgenic plant, overexpression of the GmCHR1 gene in Arabidopsis thaliana can promote the growth of Arabidopsis thaliana, significantly improve the biomass, total root length and total phosphorus content of the transgenic Arabidopsis thaliana under normal phosphorus conditions and low-phosphorus conditions; meanwhile, overexpression of GmCHR1 reduces the accumulation of flavonol in the root tip of the transgenic Arabidopsis thaliana, thereby enhancing the accumulation of auxin in the root tip of the transgenic Arabidopsis thaliana, positively regulating the synthesis of auxin in the root system of Arabidopsis thaliana and improving the content of auxin, and playing an important role in improving the growth and phosphorus efficiency of Arabidopsis thaliana.

The invention discloses a tea tree MYB transcription factor CsMYB330 gene and application, the nucleotide sequence of the MYB transcription factor CsMYB330 gene is shown as SEQ ID NO: 1, the expression of the gene participates in regulating the flavonol content in plants, and the instantaneous silencing of the CsMYB330 gene in the tea tree can significantly reduce the flavonol content in the tea tree by 24.5% and reduce the bitter taste of the finished tea.

This invention relates to compounds that are 4'-substituted analogues of the flavonol Fisetin. In particular, the invention relates to such compounds wherein the 4' position on the B-ring is substitut

This invention provides a composition of Ginkgo biloba extract and its preparation method. The composition consists of Ginkgo biloba extract (calculated as total flavonol glycosides) and dipyridamole in a dosage ratio of 10:4. The preparation method can significantly improve product yield, reduce production costs, and significantly increase the content of the main components of the produced Ginkgo biloba extract, namely total flavonol glycosides and terpene lactones. It can also reduce the content of poorly water-soluble ginkgolides and harmful impurities such as ginkgolic acid, control its microbial level, and improve the stability and safety of the Ginkgo biloba extract preparation product of this invention.

The application discloses a thiazole-containing flavonol derivative and a preparation method and application thereof, and belongs to the technical field of pesticide synthesis. A thiazole-containing flavonol derivative is generated by substitution reaction of 3-(bromoalkyloxy)-7-substituent-2-(4-substituent phenyl)-4H-chromone-4-ketone and 5-substituent-2-thiol-1,3,4-thiadiazole, and a structural formula is shown as formula Y: wherein n is 3 or 4; R1 is hydrogen or an amino group; R2 is hydrogen, an alkyl group or an alkoxy group; and R3 is hydrogen, a halogen, an alkyl group or an alkoxy group. The thiazole-containing flavonol derivative has good bacteriostatic activity and can be applied to the preparation of bacteriostatic medicaments.

The invention provides a composition of ginkgo biloba extract and a preparation method thereof, the composition is composed of ginkgo biloba extract (based on total flavonol glycosides) and dipyridamole according to a dosage ratio of 10: 4, and the preparation method can significantly improve the product yield, reduce the production cost and improve the product quality. The ginkgo biloba extract prepared by the method has the advantages that the content of main components, namely total flavonol glycosides and terpene lactones, of the ginkgo biloba extract is obviously increased, the content of bilobalide with poor water solubility and the content of a harmful impurity, namely ginkgolic acid can be reduced, the microbial level of the ginkgo biloba extract is controlled, and the stability and safety of the ginkgo biloba extract preparation product are improved.

The present invention discloses a flavonol aromatic ether light-up type fluorescent probe for detecting hydrogen sulfide and its preparation method and application. The present invention uses 7-(4-(diphenylamino)phenyl)-2-(furan-2-yl)-3-hydroxychromone as raw material, and carries out etherification reaction with 2,4-dinitrofluorobenzene to prepare a flavonol aromatic ether light-up type fluorescent probe. The flavonol aromatic ether light-up type fluorescent probe can react with hydrogen sulfide quickly and exclusively at room temperature. Under 365nm ultraviolet light, the solution fluorescence color changes from colorless to orange-red, the minimum detection limit is 96nM, and the response time is 3min. Therefore, the flavonol aromatic ether light-up type fluorescent probe has good application prospects in detecting hydrogen sulfide.

The invention relates to a flavonol compound dysosmaflavonoids I as well as a preparation method and application thereof, and can effectively solve the problems of preparation of the flavonol compound and preparation of antitumor drugs, the flavonol compound is dysosmaflavonoids I extracted and separated from dysosmaflavonoids I, has a growth inhibition effect on human liver cancer cells HepG2 and human colon cancer cells SW480, and can be used for preparing antitumor drugs. And the method has good practical application value.

The invention discloses a flavonol derivative containing a benzimidazole active fragment and application of the flavonol derivative. The flavonol derivative has the advantages of being low in raw material cost, simple and convenient in synthetic route and the like. The obtained flavonol derivative has an excellent bacteriostatic effect on botrytis cinerea and can be used for preventing and treating botrytis cinerea diseases.

The present application belongs to the technical field of quality control of traditional Chinese medicine, and particularly relates to a quality control method of Ledum palustre L. The quality control method provided by the present application comprises the following processes: S1, identification: thin layer chromatography identification is respectively performed by taking ursolic acid, hyperoside, rutin, aesculetin and aesculin as the control; S2, inspection: the moisture content, total ash content, acid insoluble ash content and alcohol-soluble extract content of the medicinal material are determined; S3, content determination: the total content of volatile oil, three five-ring triterpenes (betulinic acid, oleanolic acid and ursolic acid) and two flavonoid glycosides (hyperoside and preussin) in the medicinal material is determined by using high performance liquid chromatography. The present application combines multi-index component analysis and thin layer chromatography identification, has strong specificity and good reproducibility, can comprehensively and effectively control the quality of Ledum palustre L., and ensures the medicinal effectiveness and safety of Ledum palustre L.

The invention relates to the technical field of coreopsis tinctoria refined components and preparation and application, and provides a coreopsis tinctoria refined component and a preparation method and application thereof.The coreopsis tinctoria refined component comprises, by mass, 11 specific components of phenolic acids, flavonol, flavanol and flavonoid, and the 11 specific components comprise, by mass, 2 parts of gallic acid, 1 part of potassium sorbate, 1 part of potassium sorbate, 1 part of potassium sorbate and 1 part of potassium sorbate. The health-care food is prepared from the following raw materials in parts by weight: 3 parts of chlorogenic acid, 14 parts of quercetin, 18 parts of catechin, 5 parts of citric acid, 2-3 parts of procyanidine B, 3 parts of (+)-epicatechin, 1-31 parts of 31 parts of procyanidine C, 14 parts of rutin, 4 parts of camphanol-3-O-rutinoside and 2 parts of isorhamnetin-3-O-rutinoside. The snow chrysanthemum is Changbai Mountain snow chrysanthemum. Animal experiments show that the refined component can significantly reduce the content of total cholesterol and triglyceride in serum of a high-fat model rat, the specific component with blood fat regulating activity is prepared from Changbai Mountain snow chrysanthemum, and a new choice is provided for non-drug intervention of dyslipidemia.

The application discloses a method for catalyzing sodium borohydride to produce hydrogen at low temperature, which is characterized by using natural flavonoids (including flavone, flavonol, isoflavone and the like) as a catalyst, using methanol or a mixture of methanol and water as a reaction solvent, using NaBH4 as a raw material for hydrogen production, and performing alcoholysis of NaBH4 to produce hydrogen. The catalyst used in the application is abundant in source and low in price, can catalyze sodium borohydride to produce hydrogen through alcoholysis at a wide reaction temperature range, has stable catalytic performance, high hydrogen production rate, good catalytic durability and reusability, and has important significance for the application and popularization of sodium borohydride in the field of hydrogen production.

This invention belongs to the field of pharmaceutical technology and relates to a preparation process of a modified Huoxiang Zhengqi compound. The preparation process is as follows: Pinellia ternata and Angelica dahurica are percolated; Pogostemon cablin, Perilla frutescens, Citrus reticulata peel, Magnolia officinalis, and Atractylodes macrocephala are extracted in groups for 2-5 hours, and the total volatile oil is collected. The residues of each group are decocted with water, and the decoctions of each group are collected; Poria cocos, Platycodon grandiflorus, Glycyrrhiza uralensis, Areca catechu peel, Ziziphus jujuba, and Zingiber officinale are decocted with water to obtain a decoction; the total volatile oil is added to an inclusion material, and inclusion is obtained using an ultrasonic-assisted method; the percolation and decoctions are combined, concentrated, and centrifuged to obtain a supernatant; a preservative is added to the supernatant and the inclusion material to obtain the final product. This invention extracts medicinal materials containing volatile oils in groups and uses a cyclodextrin inclusion complexation process, which not only improves the total extraction rate of volatile oils, but also increases the content of hesperidin, hydrated oxypenicillin, magnolol, honokiol, patchouli flavonol, and baicalein, while improving the taste and flavor of the flavored patchouli flavonoid compound.

The invention discloses a flavonol derivative photobase generator as well as a preparation method and application thereof, and belongs to the field of organic synthesis. The flavonol derivative photobase generator is high in base generation efficiency, ions are easily formed by the acidic group and halogenated benzyl and tertiary amine strong base such as DBN, then deionization is performed by using strong reducing agents such as lithium aluminum hydride, and due to the fact that the bond energy of a C-N bond on the benzyl is low, the high photobase generation capacity is guaranteed. The flavonol derivative photobase generator can be applied to a base-catalyzed cross-linking curing system, can remarkably reduce the curing temperature of cross-linking resin, and can be widely applied to the fields of photoetching, packaging, coating and the like.

The invention belongs to the field of fluorescent probes, and discloses a fluorescent probe for monitoring dynamic change of hydrogen peroxide in different subcellular organelles as well as a preparation method and application of the fluorescent probe, the probe takes flavonol as a mother nucleus, a hydrogen peroxide ratio type fluorescent probe PML targeting lysosome and a hydrogen peroxide ratio type fluorescent probe PZD targeting lipid droplets. The invention also provides a preparation method and application of the probe. The probe provided by the invention has the advantages of novel structure, simple synthesis method, good recognition effect on hydrogen peroxide, especially hydrogen peroxide in subcellular organelles, fast response speed, high sensitivity, reliable examination result, good biocompatibility, subcellular organelle positioning function, and wide application prospect. The probe can be applied to monitoring of hydrogen peroxide in a microenvironment in cells, and has great significance for applying a probe technology to an actual related pathological model to develop an action mechanism of hydrogen peroxide in pathology of the probe, especially in a ferroptosis process.

The invention discloses a mutant of flavanonol 4-reductase, recombinant escherichia coli for expressing the mutant and application of the mutant. According to the invention, flavanonol 4-reductase (FaDFR) from Fragaria x anassa is taken as an object, active sites and 12 sites near an active pocket are selected for saturated mutation, and a pore plate screening method is combined to finally obtain the mutant FaDFRG130C which is high in activity and can tolerate high substrate concentration. The invention aims to provide a flavanonol 4-reductase mutant, recombinant escherichia coli for expressing the mutant and application of the mutant, and provides a research basis for industrial production of anthocyanin.