The invention discloses an
aroma enhancing method for deamidating
micelle casein based on
protein glutaminase. The method comprises the following steps: adjusting the pH value of a
micelle casein system to 6.0-8.0, reacting the
micelle casein system with
protein glutaminase at 45-55 DEG C for 5-40 minutes, and then carrying out heat treatment to terminate the reaction. The
deamidation treatment can obviously improve the
deamidation degree of micelle casein from about 4% to 48% or above, and
glutamine residues are converted into
glutamic acid, so that the physicochemical properties of
protein are effectively changed, the binding capacity of the protein and
flavor molecules is weakened, and the release and retention of fragrance are promoted. Experiments show that the headspace concentration of the
total volatile aroma substances of the treated sample is remarkably increased, the
aroma profile is optimized, specifically, the relative proportion of esters and lactones with sweet aroma,
frankincense and fruit aroma is greatly increased, and meanwhile, the unpleasant
odor is weakened. The method is simple in process, mild in condition and suitable for
flavor quality improvement of various dairy products.