233 results about "Intramuscular fat" patented technology

The present invention provides a synthetic, poorly crystalline apatite (PCA) calcium phosphate containing a biologically active agent and / or cells (preferably tissue-forming or tissue-degrading cells). The compositions provided by the present invention are useful for a variety of in vivo and in vitro applications, including drug delivery (for example, to bony sites, the central nervous system, intramuscular sites, subcutaneous sites, interperitoneal sites, and occular sites) tissue growth (preferably bone or cartilage) osseous augmentation, and methods of diagnosing disease states by assaying tissue forming potential of cells isolated from a host. The invention also provides methods of preparing delivery vehicles, of altering delivery vehicle characteristics, and of delivering biologically active agents to a site. The invention further provides in vitro cell culture systems and cell encapsulation materials. The invention is useful for both medical and veterinary applications.

The invention discloses a method for breeding a new species of lean meat type pigs-Shennong No. 1, which includes: A) the erhualian sow as female parent and the yorkshire boar as male parent are in cross breeding to obtain the yorkshire*erhualian cross bred sow; then, cross breeding female parent is in cross breeding with the danish landrace boar to obtain danish*( yorkshire*erhualian) ternary hybrid pigs; B) a fundamental selective breeding group of danish*( yorkshire*erhualian) ternary hybrid pig is formed and selected as zero-generation of Shennong No. 1; C) the boars and sows are mated to obtain piglets, good piglets are selected gradually for seed reservation; D) the good piglets are continuously cultivated until sexual maturity, the outstanding individual female and male pig are reserved as the next generation of swine respectively according to reproductive performance and meat quality as well as carcass performance and growth performance; E) the step C) and D) are repeatedly operated for 4-15 times. The main selective breeding indexes of the Shennong No. 1 pig are based on the reproductive performance and meat quality, the Shennong No. 1 pigs have the advantages of good meat quality, which is manifested in red meat color, good water holding capacity, high intramuscular fat, and good tenderness and the like. Referring to the reproductive performance, the number of the first-born Shennong No. 1 sow is more than ten.

The invention discloses a method for reducing production cost of high-end beef, which belongs to the technical field of feed nutriology in animal husbandry. During meat improvement and fishing of high-end beef cattle, part of barley and part of soybean meal in concentrated feed are replaced with corn, pomace, cottonseed meal and corn distillers grains, and rice straw in coarse feed is replaced with dried corn stalks or dried wheat straws, so that feed sources can be effectively expanded during meat improvement and finishing of high-end beef cattle, and production cost can be lowered by more than 10%. In addition, significant effects on beef cattle weight increment, feed conversion rate, beef color, beef nutrients, intramuscular fat content, variety and content of unsaturated fatty acids, beef tenderness, marbling level and the like are absent. The breeding cost can be saved distinctly, local feed sources can be fully utilized, and accordingly the method is widely applicable to high-end beef fattening.

The invention belongs to the fields of molecular biological technology and molecular marker technology and relates to an SNP molecular marker related to intramuscular fat content of a Duroc boar. Thesite of the SNP marker is the 117427086th nucleotide site on the chromosome 7 of the International Pig Reference Genome 11.1 version, and the basic group of the site is G or A. By preferably selectingthe dominant allele of the SNP, the frequency of the dominant allele can be increased generation by generation, the intramuscular fat content of the boar can be increased, and the genetic improvementprogress of the boar can be accelerated, thereby effectively improving the economic benefit of boar breeding.

Dual-energy absorptiometry is used to estimate intramuscular adipose tissue metrics and display results, preferably as related to normative data. The process involves deriving x-ray measurements for respective pixel positions related to a two-dimensional projection image of a body slice containing intramuscular adipose tissue as well as subcutaneous adipose tissue, at least some of the measurements being dual-energy x-ray measurements, processing the measurements to derive estimates of metrics related to the intramuscular adipose tissue in the slice, and using the resulting estimates. Processing the measurements includes an algorithm which places boundaries of regions, e.g., a large region and a smaller region. The regions are combined in an equation that is highly correlated with intramuscular adipose tissue measured by quantitative computed tomography in order to estimate intramuscular adipose tissue.

The invention discloses a feedstuff for pigs in the later period of fattening and belongs to the technical field of cultivation; the ingredients and the percentage of the feedstuff for pigs in the later period of fattening are: 57 to 60 percent of corn, 13 to 14 percent of soybean pulp, 22 to 24 percent of bran, 0.5 to 1.5 percent of fish meal, 0.4 to 0.5 percent of salt, 2.7 to 3.2 percent of zeolite and 0.0075 to 0.009 percent of compound vitamins; experiment proves that in the later 10 to 20 days of fattening, the use of the feedstuff for pigs of the invention has no effect on the growth performance and carcass composition, the quality of pork is better with better index of pork color, marbling, pH, intramuscular fat content, rate of cooked pork and diameter of muscle fiber, etc., and the quality of the muscle of pigs has a certain improving effect; the contents of calcium, phosphorus and copper in liver and muscle are reduced with different degrees, especially the content of the copper in the muscle is reduced by 25.17 to 40.21 percent obviously, and the cost of the feedstuff is low.

The invention discloses an antibiotic-free feed for fattening pigs, belonging to the technical field of feeds. The antibiotic-free feed comprises a feed used during a fattening period and a feed used before slaughter, wherein the feed used during the fattening period is prepared from the following materials by weight percent: 54-58 percent of corn, 17-19 percent of soybean meal, 10-14 percent of wheat germ, 8-10 percent of wheat middling, 1 percent of composite additive, 3 percent of premixed materials and 1 percent of trace elements; and the feed used before slaughter is the feed used during the fattening period without adding the trace elements. By adopting the feed, the application of antibiotics can be avoided, the metal level in the produced pork is greatly reduced, the meat color score is improved by 14.55 percent, the marbling score is improved by 9.09 percent, the fat content in muscle is improved by 21.05 percent, and the rate of water loss is reduced by 32.98 percent. By using the feed provided in the invention, the daily gain and the survival rate of the fattening pigs are significantly increased, the feed conversion ratio is reduced, the body weight for slaughter is increased, and feeding days and age in days for slaughter are significantly reduced. In addition, the preparation method disclosed in the invention is simple, and the production cost is low.

The apparatus for measuring the intra-muscular fat in carcasses or parts of carcasses or the total content of fat in minced meat has a first pair of electrodes for insertion into a carcass or part of a carcass or into minced meat. An alternating current (AC) generator is connected to these electrodes. Second and third pairs of electrodes are inserted parallel to the first pair. Both the second and third pairs have measurement circuits to measure the voltage and phase between the electrode pairs after insertion. The needle or bar shaped electrodes are tapered and insulated apart from 5-20 mm long free end sections. Electrodes are separated by 10-100 mm with each electrode 10-50 mm from the nearest of the first electrode pair. The portable unit for on-line use has a display output. The AC frequency applied to the first electrode pair may be changed in an exponential series to give different measurements.

The invention belongs to the technical field of preparation of molecular markers of livestock and in particular relates to location of an SNP molecular marker related to the intramuscular fat content of pigs and application of the SNP molecular marker. The SNP molecular marker is obtained from whole genome correlation analysis, and the nucleotide sequence of the SNP molecular marker is as shown in the sequence table SEQ ID NO:1 and figure 2. The marker can be used in the correlation detection of the intramuscular fat content characters of the pigs.

The invention discloses a molecular marker influencing the intramuscular fat character of a pig and application, and belongs to the field of molecular biological technologies and genetic breeding. The molecular marker is located at the 23147683rd locus of the nucleotide sequence of the eighth chromosome of the pig, the nucleotide sequence of the molecular marker is shown in SEQ IDNO:1, and the G>A allelic mutant is located at the 400th nucleotide of the genomic sequence of the pig. The new marker is provided for the genetic improvement of the pig, and the genetic breeding work of the pig of the good strain is facilitated.

The invention discloses a mating system selective breeding method for producing a high-quality commercial pig, comprising the following steps: A) taking halothane-free recessive gene Pietrain as a first male parent of a mating system, taking Duroc as a first female parent of the mating system, and hybridizing to obtain Pierre cross-bred pig; and B) selecting the Pierre cross-bred pig as a second male parent of the mating system, taking Shannon No. I pig as a terminal female parent, and hybridizing to obtain the high-quality commercial pig. The average litter size of the pluriparous sows of the mating system terminal female parent of the invention is not less than 12, the growth speed of the high-quality commercial pig is up to above 700 g / d, lean meat percentage is above 62%, feed conversion ratio is not higher than 3.2, intramuscular fat content is not lower than 2.5%, and no PSE meat exists.

The invention provides a microbial preparation for fermentation production of a fodder. The microbial preparation comprises the components, in parts by weight: 2-20 parts by weight of yeast, 15-60 parts by weight of bacillus, and 30-60 parts by weight of lactic acid bacteria. The microbial preparation provided by the invention acts on a fodder raw material and performs solid fermentation to prepare a microbial fermented fodder which can improve the quality of mutton, the microbial fermented fodder is more easily absorbed and utilized by stomach, so that ultimately the growth rate of meat sheep can be accelerated, the sheep can be slaughtered early, the water loss rate of the mutton is obviously reduced, and the intramuscular fat content and the intramuscular amino acid content are significantly increased.

The invention provides a feed for pork rich in omega-3 fatty acid and a preparation method thereof, which is characterized in that the feed improves omega-3 fatty acid content ratio in pig intramuscular fat, back fat, abdomen fat as well as heart and liver fat for 3-7 times, and pork rich in omega-3 fatty acid is not easy to oxidize and has long storage time. The feed is prepared by the following componets 10% of flaxseed, VE 200 / kg diet and 1% of Chinese herbal medicine are added in formulating diet, wherein the Chinese herbal medicine is prepared by fructus ligustri lucidi and Astragalus mongholicus at the ratio of 1: 1. The method comprises the following steps: screening flaxseed, selecting VE, preparing compound Chinese herbal medicine, providing a diet formula, and preparing diet. The feed disclosed by the invention can improve omega-3 fatty acid content ratio in pig intramuscular fat, back fat, abdomen fat as well as heart and liver fat for 3-7 times, and pork rich in omega-3 fatty acid is not easy to be oxidized and has long storage time.

The invention relates to a breeding method for improving the lean meat percentage of Meishan pigs and belongs to the field of animal genetic breeding. A Danish landraces as a male parent and a Meishan pigs as a female parent are hybridized in a three-way mode. Offspring breeding standards comprise the following standards: clothing hair is white, the total number born is larger than 14, the lean meat percentage is larger than 55%, and the daily gain is more than 630g. Suzhong pig generation II bred by the breeding method retains the characteristics of high total number born, good meat quality (intramuscular fat is larger than 3%), coarse feed resistance and easy feed of the Meishan pigs, the daily gain is increased to 634.74 grams from 439 grams and is increased by 44.59%, and the lean meat percentage is increased to 54.49% from 42.8% and is increased by 27.31%. The characteristics of fast growth, high lean meat percentage, and the like of the landraces are also retained, the total number born is increased to 14.24 from 11 and is improved by 29.5%, and the meat quality is also improved obviously.

The invention relates to a feed additive capable of improving the flavor of pork as well as a preparation method and application of the feed additive. The feed additive comprises the following components of phytosterin, a plant extract, selenium yeast, probiotics powder, conjugated linoleic acid, saccharicterpenin and a carrier substrate. The preparation method comprises the following steps of weighing the components in parts by weight, firstly screening the plant extract, and performing uniform mixing; then mixing the phytosterin with a part of the carrier substrate; and finally, adding the remaining components, performing sufficient and uniform mixing, performing granulation, and performing screening. Compared with the prior art, the feed additive disclosed by the invention has the advantages that after the feed additive is added to a pig basic ration, the growth of pigs can be promoted, the feed utilization rate can be increased, the eye muscle area and the lean meat percentage canbe increased, the water drop losses can be reduced, and the content of intramuscular fat of the pork can be adjusted and controlled, so that the intramuscular fat is rich and uniform, the compositionstructure and the balanced proportion of fatty acid in the pork are improved, the fat percentage is reduced, the content of flavored precursor substances of the pork is increased, the tenderness and the juicy nature of the pork are improved, and the pork is delicious in flavor, delicate in mouth feel, fresh and juicy, free from drug residues and healthy.

The invention discloses intramuscular fat related lncRNA (long non-coding Ribonucleic Acid) and application thereof. The invention finds out XLOC_004398 related to intramuscular fat of pork; a targetgene NAP1L3 for differentially expressing the lncRNA is analyzed and predicated through co-expression network analysis and a trans regulation effect and is verified. The invention provides certain evidences for breeding high-meat-quality livestock and poultry varieties and treating and preventing fat metabolism related diseases, and a new target is explored.

The invention relates to a primer pair for an SNP marker related to content of intramuscular fat of a Suhuai pig and an application thereof. The SNP marker is located on a nucleotide sequence of an SCD gene on a chromosome 14 of the pig; a site of the SNP marker is a molecular marker of a rs80912566 nucleotide site contained in the chromosome 14 of the pig in a reference sequence of an international pig genome 11.1 version, and the rs80912566 nucleotide site has C / T polymorphism; and the SNP marker is significantly correlated with the content of the intramuscular fat of the Suhuai pig (P<0.05). and the primer pair is used for detecting the SNP marker, an upstream primer is SEQ ID NO:2, and a downstream primer is SEQ ID NO:3. The SNP marker is related to the intramuscular fat of the Suhuaipig; the SNP marker can be used to screen Suhuai pig strains with high intramuscular fat; and the obtained Suhuai pig strains with the high intramuscular fat has important economic and social values.

The invention discloses a biological feed product for improving the content of intramuscular fats in pork, and a formula thereof. The above biological compound feed comprises 65-75wt% of corn, 10-20wt% of soybean meal, 8-15wt% of fermented bioprotein and 3-8wt% of a feed additive. A production method of the compound feed comprises the following steps: fully mixing all the above materials according to the above weight percentage proportions, and carrying out metrological packaging. The biological feed prepared through the formula contains no antibiotics, can substantially improve the content of intramuscular fats in pork, improves the quality of pork products, and improves the growth speed of pigs and the stress resistance of pig groups. The method has the advantages of simplicity, no special equipment, and suitableness for industrialized production.

The invention discloses a making method of mastic steamed meat, and belongs to of the technical field of food processing. Powder steamed meat is a traditional famous dish in China, which is prepared from meat with skin, rice and other auxiliary materials in parts by weight as follows: 100-150 of intramuscular fat, 40-60 of rice meal, 0.4-0.6 of salt, 0.3-0.5 of gourmet powder, 0.4-0.6 of white sugar, 0.3-0.5 of pepper, 0.4-0.6 of garlic powder, 0.3-0.5 of ginger powder, 0.2-0.3 of prickly ash powder, 0.2-0.3 of star aniseed powder, 0.3-0.5 of powdered cinnamon, 0.3-0.5 of chili powder, 7-10 of fermented bean curd, 0.4-0.5 of recombination phosphate, 1.2-1.8 of soy, 0.02-0.03 of monoscus red and 30-50 of ice water. According to the invention, the selected high quality marbled meat is cut into slices, the sliced marbled meat is put in a tumbling machine so as to be kneaded with the other auxiliary materials according to the weight ratio in a rolling manner, the pickle is carried out for 2-3 hours, then the packaging is performed, the powder steamed meat is fed into a refrigerator to preserve. The production process is simple, and the powder steamed meat is better applied to mass production; and a freezing storage method is adopted to greatly prolong a quality guarantee period, consumers only need to defrost and steam to eat after buying, the taste is delicious, the meat incense assails the nostrils, and the powder steamed meat is popular with the consumers.

The invention belongs to the technical field of pig genetic marker preparation and particularly relates to a genetic marker associated with pig meat quality and carcass characters, wherein the marker is obtained by screening the pig BMP-3b gene, and the sequence is shown by SEQ ID NO:1 and figure 4; and A / G base mutation happens at the 425th-site base of the sequence and causes AlwNI-RFLP polymorphism. The screening method comprises the following steps: extracting genome DNA from pig blood; designing a primer; performing PCR amplification; cloning the PCR product and sequencing; comparing and analyzing the sequence; performing SNP detection; and analyzing the correlation between the marker and the meat quality characters. The invention discloses an SNP typing detection technology for the CDS area of the pig BMP-3b gene, and significant correlation is discovered between the A / G mutation site and the meat quality characters such as marbling, color value and intramuscular fat content and between the A / G mutation site and the carcass characters such as average back-fat thickness, eye muscle height and leaf fat. The invention provides a new marker resource for pig marker assisted selection.

The invention discloses a nucleotide sequence or an amino acid sequence of a PHKG1 (phosphorylase kinase, gamma 1) gene located on pig chromosome 3, wherein a mutation site of the PHKG1 gene is the C-A mutual mutation of a mononucleotide at the 8118th site of a sequence shown in SEQ ID NO: 1; when C is mutated to A, a normal transcript generated by the PHKG1 gene in a transcription process deletes abnormal transcripts of 32 bp fragments, and the deleted 32 bp fragments are located at the 8123rd-8154th base of 5' end of the sequence shown in SEQ ID NO: 1; on the basis, disadvantageous genotype individuals are eliminated by selecting advantageous genotype individual reserve breeding pigs, so that population meat quality traits can be remarkably improved, especially, muscle glycogen content can be reduced, drip loss and processing loss can be reduced, and pH value and intramuscular fat content can be increased, thus the pig meat quality is improved.

The invention discloses a commercial strain method utilizing local pig breeds to breed high-quality commercial pigs, comprising the following steps: A) utilizing a freon-free recessive gene Pietran as a first male parent of the commercial strain and Fengjing pig as a first female parent of the commercial strain, hybriding to obtain a Pietran-Fengjing cross-bred pig; and B) selecting a Duroc as a second male parent of the commercial strain and the Pietran-Fengjing cross-bred pig as a terminal female parent, hybriding to obtain the high-quality commercial pig. The average farrowing number of the first female parent of the commercial strain is not less than 14, the growth rate of the high-quality commercial pig can be up to more than 600 g / d, the lean meat percentage is more than 60%, the feed conversion rate is not more than 3.5, the intramuscular fat content is more than 2.5%, and the high-quality commercial pig has no PSE meat.

The present invention relates to a pork intramuscular fat content nondestructive testing method based on computer vision. The method comprises the steps of adopting a camera calibration method to calibrate a CCD digital camera, selecting the pork musculi oculi just now purchased from a slaughter house as an experiment sample, shooting and sampling the cross-section of the pork musculi oculi, carrying out the pre-processing on an obtained sample image via an improved sample block repair method, and recovering the original information of a light reflection area of the image; combining a maximum entropy method and an iteration method to carry out the image segmentation on the pre-processed musculi oculi image, and extracting the pork marbling; extracting 291 characteristic values, such as a fat number index, a fat distribution index, a fat texture index, etc., from an obtained marbling image, establishing a pork intramuscular fat content prediction model according to the characteristic values and a chemical method detection result, and finally predicting the pork intramuscular fat content via the model. By utilizing the method of the present invention, the pork intramuscular fat content can be predicted very well, and the pork nutrition detection is more objective, more accurate and more efficient.

Aspects of the present invention also provide novel compositions and methods based on novel PAPD1 single nucleotide polymorphisms selected from the group consisting of AAFC02034082.1:g.9224G>A, g.9330A>C, g.9367G>C, g.9419A>C, g.9435T>G, g.9409[(G)4]+[(G)5], g.10084T>C, g.10228G>T, c.10326C>T and c.10364A>G, which may provide novel markers for intramuscular fat deposition. Additional aspects provide for novel methods which may comprise marker-assisted selection or marker-assisted management to improve intramuscular fat deposition in cattle.

The invention discloses a cross-breeding method for commercial pigs possessing Meishan pig blood lineage and Berkshire pig lineage. The cross-breeding method comprises the steps of cross-breeding Meishan pigs with Berkshire pigs to breed commercial pigs, or cross-breeding later generations bred by Berkshire boars and Meishan sows with the Meishan boars to breed commercial pigs. The Berkshire pig lineage of foreign pig variety is introduced to appropriately improve the growth speed and lean meat percentage of the cross-bred commercial pigs while an excellent meat quality characteristic and a reproductive characteristic of the Meishan pigs are kept. The growth speed of the commercial pigs is 480 g-600 g every day, the lean meat percentage of each slaughtered pig which is about 80 kilogram ranges from 45% to 55%, the feed conversion rate is 3.3-3.8: 1, the meat color grade ranges from 3.0 to 4.5, and the intramscular fat content ranges from 3.5% to 5.0%.

The invention discloses a high-quality broiler chicken inosinic acid and intramuscular fat content related gene polymerization breeding method comprising the following steps: respectively amplifying a gene by using three pairs of primers under the condition of PCR (Polymerase Chain Reaction) by taking two-continuous-generation high-quality broiler chicken genome DNAs (Deoxyribose Nucleic Acids) as templates; judging the sizes of amplification products of the first primer, the second primer and the third primer according to agarose gel electrophoresis, and then, screening the mutation of three alkali bases by using a DNA sequencing technology, wherein the three alkali bases include an exon 9 of an adenylosuccinate lyase gene, a non-coding region of a phosphoribosylglycineamide synthetase-5-aminoimidazole ribonucleotide synthetase-phosphoribosylglycineamide transmethylase gene 5' and an intron 2 of an adipocyte fatty acid binding protein gene; and then, carrying out gene typing and gene frequency analysis on SNPs (Single Nucleotide Polymorphisms) of the three loci by using polyacrylamide gel electrophoresis. By using the high-quality broiler chicken inosinic acid and intramuscular fat content related gene polymerization breeding method, the favorable polymerization of a protogene type can be realized, and meanwhile, the genetic mechanisms of the chicken meat quality, flavor and character can be searched more comprehensively.

The invention discloses a method for breeding a high-quality pig complete set line by aid of Tibetan pigs. The method includes steps: (1) the Tibetan pigs and Meishan pigs serve as breeding materials to breed a synthetic line, namely a Tibetan-Meishan synthetic line, and the Tibetan-Meishan synthetic line serves as female parents of the complete set line; (2) Berkshire pigs serve as the first male parents of the complete set line, the Tibetan-Meishan synthetic line serves as the female parents, and sows of the parents' generation of the complete set line are obtained after hybridization; (3) durocs serve as terminal male parents to be hybridized with the sows of the parents' generation to obtain high-quality commercial pigs of the complete set line. Pigs in the complete set line bred in the method are identical in body forms and appearances, hair on the whole pig bodies is black, and brown pigs and white pigs are few; heads of the pigs are light, mouths of the pigs are straight, and ears of the pigs are median in size; backs and bellies of the pigs are flat and straight, body and limb combination is good, and legs and buttocks are well developed. The meat quality is good, production efficiency is high, weight of the pigs can reach 90 kg at 187.22 days of age, the feed conversion rate is 3.14, the lean meat percentage is 57.72%, and the intramscular fat content is 4.07%.

The invention discloses a commercial pork pig multiple crossbreeding method. The method comprises the following steps of A) taking Pietrain pig with non-halothane allogene as the first male parent of multiple crossbreeding and Meishan pig as the first female parent of multiple crossbreeding for hybridization to obtain Pietrain-Meishan-crossed hybrid pig; B) taking Duroc pig as the second male parent of multiple crossbreeding and the Pietrain-Meishan-crossed hybrid pig as the terminal female parent of multiple crossbreeding for hybridization to obtain commercial pork pig. According to the commercial pork pig multiple crossbreeding method, the average litter size of multiparity sow of the first female parent of multiple crossbreeding is not less than 14.5; the hybrid high-quality commercial pork pig obtains an average daily gain up to more than 680 g / d (gramme per day) and a lean meat rate up to more than 64% and a feed conversion rate as little as 2.75, avoids PSE (pale-soft-exudative) meat and obtains an intramuscular fat percentage up to 2.5%.

For the situation that intramuscular fat content is an important factor for influencing pork quality and flavor and an efficient and rapid living body determination method does not exist, the invention discloses a method for quickly determining intramuscular fat content of fattening duroc landrace big white. The method comprises the following steps: firstly, establishing a regression equation between back-fat thickness and intramuscular fat content of the fattening duroc landrace big white through the actual measured data; then, determining back-fat thickness of a living body to be measured, treating through the regression equation, and calculating the intramuscular fat content of the live pig. According to the invention, the regression equation between back-fat thickness and intramuscular fat content is established and is input into an intelligent processing device such as a mobile phone or a computer through one procedure, and then the intramuscular fat content is quickly estimated by determining back-fat thickness, so that a basis is provided for the fact that pigs become full grown and are ready for slaughter when the pigs are provided with proper intramuscular fat content and the pork quality is improved. Moreover, the technical scheme is low in implementation cost and quick determination is realized.