114 results about "Intramuscular fat" patented technology

The invention discloses a method for improving beef quality, an action mechanism and an experimental method, in a cattle body, vitamin A can activate the expression of EBF2 through an active metabolite RA of the vitamin A, and the EBF2 can inhibit the transcription process of CYP26B1 in a targeted manner to maintain the activity of a retinol signal channel, so that PPAR gamma and downstream lipid metabolism related genes thereof are activated, and the activity of the retinol signal channel is improved. Fatty acid transport and lipid accumulation in fat cells in the cattle muscle are promoted, fat deposition in the cattle muscle is promoted, and the beef quality is improved. By constructing the molecular network for regulating and controlling the formation of the fat in the cattle muscle, a complex regulation and control mechanism for controlling the fat deposition in the cattle muscle can be understood more deeply, a method for improving the beef quality based on regulating and controlling the vitamin A mediated key factor EBF2 is provided, and a new way is provided for improving the beef quality in a targeted manner.

The invention relates to a biological preparation for improving quality of weever, the biological preparation comprises an injection and an edible agent, the injection comprises chemically modified chemically synthesized single-stranded small RNA, Rev-erbalpha protein and Per1 protein, and the edible agent comprises Clock core peptide 1, Clock core peptide 2 and taurine. Skeletal muscle development, liver lipid metabolism and generation of cell inflammatory factors of the weever are influenced by adding and injecting the feed, so that the quality of the weever is improved, intramuscular fat deposition is promoted, the taste and flavor of fish meat are improved, the energy reserve of the weever can be improved, and the culture cycle is shortened. The improvement of cell inflammatory factors shows that the disease resistance of the weever is improved, the survival rate in the breeding process is further improved, and the use amount of antibiotics is reduced. The method provides an important theoretical basis for improving fish meat quality in China and promoting high-quality, efficient and healthy development of aquaculture, and has a wide market prospect.

The invention provides a Jinhua pork quality improvement method and system based on data mining, and relates to the technical field of intelligent breeding, and the method comprises the steps: obtaining an original data set; performing data fusion according to the original data set, and obtaining a multi-modal association graph by constructing an entity-relation network topology structure; performing potential influence path extraction according to the multi-modal association map to obtain an influence path set; performing interaction effect modeling according to the influence path set to obtain a dynamic effect model; performing meat defect tracing according to the dynamic action model to obtain a defect driving factor set; and generating an optimization strategy according to the defect driving factor set to obtain a meat quality improvement decision scheme. According to the method, the multi-factor relation network is constructed by integrating Jinhua pig full-period multi-source data, the key conduction path and the dynamic interaction model influencing meat quality formation are accurately extracted, and the nonlinear antagonism relation between the leaf fat deposition tendency and intramuscular fat accumulation under the specific physiological mechanism of the Jinhua pigs is systematically quantified.

The invention discloses a method for producing snowflake beef through yak seasonal barn feeding and short-term fattening. Belongs to the field of animal husbandry, and achieves efficient output of yak snowflake beef through short-period seasonal fattening by selecting fattening yaks, selecting fattening seasons and time and cooperating with daily ration in the fattening early stage and the fattening later stage. In the late fattening period, soybean-meal-free low-protein daily ration is adopted to synergistically promote fat deposition in yak muscles, so that efficient output of the yak snowflake beef is achieved, and the method for producing the snowflake beef by using the yaks has important significance for improving the economic benefits of yak breeding in plateau pasturing areas and meeting the requirements of the market in China for high-quality beef.

The invention belongs to the technical field of animal breeding, and particularly relates to a molecular marker closely linked with beef quality characters, a primer pair, a detection reagent and application. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, the nucleotide shown as SEQ ID NO.1 has G / A polymorphism at the 831th bp, when the genotype of the 831th site from the 5'end of the molecular marker is GG, the meat color brightness and intramuscular fat content of an individual are higher than those of a GA genotype individual, and the cooking loss of the individual is lower than that of the GA genotype individual, and the molecular marker can be used for selective breeding of high-quality beef cattle.

The invention belongs to the technical field of animal breeding, and particularly relates to application of a detection reagent of an SNP (Single Nucleotide Polymorphism) molecular marker related to pork quality traits. Basic groups at 208bp and 217bp from the 5'end of the sequence have synchronous C / T mutation, the sequence shows three genotypes of CC, CT or TT, T allele is dominant allele, and the intramuscular fat content of the TT type pig is greater than that of the CC type pig. The invention further develops a reagent for detecting the SNP molecular marker, the reagent can be used for pig assisted breeding, pig breeding, pork quality character identification or pork quality character screening, and a method for identifying the intramuscular fat content of pork and screening pork with rich intramuscular fat is developed.

The present invention discloses the application of the MYLK gene and the CPSF2 gene in regulating beef intramuscular fat deposition, belonging to the field of genetic engineering technology. By studying the APA mechanism during cattle intramuscular fat deposition, the present invention found that during the IMF deposition process, the 3′UTR of the MYLK gene shortened and the expression level of the MYLK gene increased. During the shortening process, the miRNA involved in IMF deposition was lost, thereby regulating the reduction of IMF deposition; after the 3′UTR of the CPSF2 gene was shortened, its expression was also upregulated, regulating the reduction of IMF deposition. The research results of the present invention provide new theoretical support and technical guidance for the prediction of beef quality and the breeding of high-quality beef cattle, which is of great significance to promoting the development of animal husbandry and improving the production efficiency of animal husbandry.

The invention relates to the technical field of molecular biology, and discloses a miRNA molecule for regulating and controlling fat deposition in pig muscle, one of the purposes of the invention is to provide a miRNA molecule for regulating and controlling fat deposition in pig muscle, the miRNA is SSC-miR-874, and the mature sequence of the miRNA is shown as SEQ ID NO1: CUGCCCUGGCCGAGGGACCGAC. The second purpose of the invention is to provide a product, and the product comprises SSC-miR-874 mimics or SSC-miR-874 inhibitor, and is characterized in that the SSC-miR-874 mimics and the SSC-miR-874 inhibitor are added into the product; wherein the sequences of the SSC-miR-874 mimics are as shown in SEQ ID NO1 (sequence identifier number 1) and SEQ ID NO2 (sequence identifier number 2), and the sequence of the SSC-miR-874 inhibitor is as shown in SEQ ID NO3 (sequence identifier number 3). The application reveals that the SSC-miR-874 can negatively regulate and control the proliferation and adipogenic differentiation of the precursor fat cells in the pig muscle, provides a new molecular target for deeply understanding the fat deposition mechanism in the pig muscle, provides an effective miRNA intervention strategy for optimizing the pork quality and increasing the fat content in the muscle, and has important breeding application value and industrialization prospect.

The present disclosure relates generally to methods and test kits for identifying SNPs associated with desirable meat eating quality traits in ovine animals. In particular, the present disclosure relates to a SNP-based diagnostic test and method for identifying ovine animals with desirable fat melting point (FMP), intramuscular fat (IMF) and omega-3 long chain polyunsaturated fatty acids (n-3 LC PUFAs) which are characteristic of Australian White sheep or Lamb (e.g., Tattykeel Australian White Lamb), and the use of those tests and methods in animal breeding programs.

The invention belongs to the technical field of animal breeding, and particularly relates to a molecular marker closely linked with beef intramuscular fat traits, a primer pair, a kit and application. Nucleotide sequences of the molecular marker are shown as SEQ ID NO.1 and SEQ ID NO.2, the molecular marker has T / C polymorphism from the 76th basic group of the 5'end, and when the genotype of the locus is TC, the intramuscular fat content of beef of an individual is higher than that of the individual with the genotype of TT. The beef cattle with the high intramuscular fat content character can be screened or cultivated by utilizing the molecular marker.

The invention discloses a multi-mode-based sheep non-injury intramuscular fat content detection method, system and device and a medium, and relates to the field of intramuscular fat evaluation.The method comprises the steps that an ultrasonic image of to-be-detected sheep is obtained; extracting physiological attribute characteristics of the sheep to be detected based on the ultrasonic image; a DB-KAN dual-branch network is constructed and trained; the DB-KAN double-branch network comprises a CNN (Convolutional Neural Network) branch, a Transform branch and a regression head based on the KAN; and inputting the ultrasonic image and the physiological attribute characteristics into a trained DB-KAN double-branch network to obtain the intramuscular fat content of the sheep to be detected. The intramuscular fat content can be rapidly and accurately detected in a non-invasive manner.

The application discloses a SNP marker related to an intramuscular fat content character of Rongchang pigs, a detection primer and application, and belongs to the technical field of molecular biology. The application develops a SNP marker and a detection primer related to the intramuscular fat content character of Rongchang pigs based on a SNP marker technology. The SNP marker developed by the application is located at 121626638 bp of a No. 7 chromosome of Rongchang pigs, and relates to C / T mutation. When the genotype is CT, the intramuscular fat content of Rongchang pigs is significantly higher than that of the TT genotype. The SNP marker can screen out individuals with excellent intramuscular fat content, so that the level of the intramuscular fat content character of Rongchang pigs is improved, and the breeding process of new breeds is accelerated.

The application provides a method for optimizing the slaughter weight of Jinfen white pigs to produce high-quality flavor pork. Through systematic research on the influence of different slaughter weights on the pork quality and flavor of Jinfen white pigs, it is first determined that when the slaughter weight is 135.08±1.10 kg, the pork has the optimal comprehensive quality performance, which is specifically embodied in that the intramuscular fat content reaches (3.82±0.05)%, the shear force is (35.75±1.68) N, and the meat quality is excellent. At the same time, the pork of the weight group has the most total volatile substances, and the types of various volatile substances are relatively rich, and exhibits the best flavor characteristics. The application not only provides clear process parameters for the production of high-quality flavor pork of Jinfen white pigs, but also provides a reliable technical basis for the precise regulation of meat quality based on the slaughter weight.

This invention belongs to the field of molecular marker-assisted selection technology for pig breeding and pigs, specifically involving molecular markers related to pork quality traits and their applications. This invention will be located in... PPP3CB 5' flanking promoter region of the gene InDel Polymorphic sites are associated with pork quality traits including water loss rate, water holding capacity, and intramuscular fat. InDel When the genotype at the polymorphic locus is TT, the pork has a lower water loss rate, a higher water holding capacity, and a higher intramuscular fat content. InDel InDel When the genotype of a polymorphic locus is deleted, the pork has a higher water loss rate, lower water holding capacity, and lower intramuscular fat content. This invention enables early prediction and selection of meat quality traits that can only be measured after slaughter, during the early stages of pig growth and development. It not only allows for live detection and screening of pork quality traits but also significantly shortens the generation interval, accelerates the breeding process, and provides technical support for early pig breeding.

The application discloses a SNP marker combination for broiler breast intramuscular fat content prediction and assisted breeding and application thereof, and belongs to the technical field of assisted breeding.The application aims to provide a method for broiler breast intramuscular fat content prediction and assisted breeding.The application provides 331 SNP markers related to broiler breast intramuscular fat content, which are used for identifying broiler breast intramuscular fat content.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker of an eleventh intron of a pig ARRB1 gene, a primer pair and application of the SNP molecular marker and the primer pair in pork quality character breeding, and relates to the technical field of pig molecular markers. Wherein the pork quality character is intramuscular fat character; the SNP molecular marker is located in an eleventh intron of an ARRB1 gene of a ninth chromosome of a pig, the nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.1 in a sequence table, and g.130Cgt exists at the 130bp position of the sequence; the T basic group is mutated. The invention also provides a primer pair for amplifying the molecular marker and a method for breeding pigs by using the molecular marker. According to the invention, the correlation between one SNP molecular marker in the pig ARRB1 gene and the intramuscular fat character of the pig is explored for the first time, so that the SNP molecular marker can be used for marker-assisted selective breeding of the pig, that is, a new application of the SNP molecular marker in the pig ARRB1 gene in marker-assisted breeding of the pork quality character is provided, early screening of the breeding character of the pig is realized, and the breeding efficiency of the pig is improved. The screening method is simple and rapid.

The invention belongs to the technical field of microorganisms, and discloses clostridium butyricum ML5358 and application thereof.The clostridium butyricum ML5358 provided by the invention has the preservation number of CGMCC No.37000. The strain can effectively relieve mouse colitis induced by DSS and remarkably reduce expression of colon tissue inflammatory factors Il-6 and Il-1beta, and the preservation number of the clostridium butyricum ML5358 is CGMCC No.37000. The clostridium butyricum ML5358 is CGMCC No.37000. The clostridium butyricum ML5358 is CGMCC No.37000. In addition, skeletal muscle lipid metabolism can be regulated and controlled through the intestine-muscle axis, expression of fat synthesis and uptake related genes Srebf1, Acc1 and Fabp4 is specifically up-regulated, the content of intramuscular triglyceride is remarkably increased, and intramuscular fat deposition is promoted. According to the clostridium butyricum ML5358 and the application thereof, the functional limitation of traditional clostridium butyricum is broken through, and a new strain resource is provided for developing a novel microbial preparation or a feed additive capable of improving intestinal health and improving livestock and poultry meat quality.

The invention discloses a compound enzyme preparation-containing nutritional feed for regulating and controlling intramuscular fat of black pigs and a preparation method of the compound enzyme preparation-containing nutritional feed, and relates to the technical field of livestock and poultry breeding feeds. The invention discloses a black pig skeletal muscle cell fat directional transport feed which is prepared from the following components in parts by weight: 850-920 parts of basal feed, 5-12 parts of fat directional deposition compound enzyme group, 25-40 parts of amino acid-enzymatic synthesis substrate combination and 2-6 parts of lipolytic enzyme specific inhibitor. According to the black pig skeletal muscle cell fat directional transport feed, fat precursor directional transport is realized through the compounded fat directional deposition compound enzyme group, a fat synthesis pathway targeting black pig skeletal muscle cells and lipoprotein lipase; acetyl-coenzyme A carboxylase and fatty acid synthetase catalyze fat synthesis step by step, only intramuscular fat generation pathways are activated, subcutaneous and abdominal fat deposition paths are blocked, the technical defects that fat deposition of an existing single enzyme preparation is non-directional, and the body fat rate is caused to be abnormally increased are overcome, meanwhile, an exclusive amino acid-enzymatic synthesis substrate combination is matched, and the body fat synthesis efficiency is improved. Sufficient adaptive substrates are supplied to enzyme group catalytic reaction, and the problem that a single enzyme preparation is insufficient in catalytic efficiency is solved.

The invention belongs to the technical field of veterinary feed, and particularly relates to a feed additive for improving pork quality and a preparation method thereof. The composition is prepared from the following raw materials in parts by weight: 30-40 parts of conjugated linoleic acid, 12-15 parts of gelatin, 10-15 parts of whey protein, 12-15 parts of yeast beta-glucan, 8-12 parts of butyrin, 6-7 parts of an antioxidant, 8-10 parts of chitosan and 40-60 parts of maltodextrin. According to the compound feed additive disclosed by the invention, through the synergistic effect of the conjugated linoleic acid microcapsules, the beta-glucan-butyrin compound and the tea polyphenol-chitosan compound, CLA slow release regulation and control of fat metabolism are realized, and the lean meat percentage and intramuscular fat are increased; beta-glucan can enhance intestinal immunity, and butyrin can promote nutrient absorption; the tea polyphenol-chitosan compound resists oxidation and improves the meat color and the water-retaining property, conventional methods such as spray drying and low-temperature mixing are adopted in the process, and the cost is effectively reduced.

The invention discloses a synthetic microbial flora for improving pork quality and application thereof. The synthetic microbial flora comprises two kinds of probiotics, namely lactobacillus plantarum WY2401 with the preservation number of CCTCC (China Center For Type Culture Collection) NO. The invention relates to ackermann muciniophilic bacteria. The synthetic microbial flora is prepared by compounding lactobacillus plantarum and ackermann mucophile according to a colony formation unit number ratio of 1: 1, concentrating and freeze-drying. Experimental results show that the synthetic microbial flora is added into the basic feed of the fattening pigs, so that the carcass traits are improved, the intramuscular fat content is increased, the conversion of muscle fiber types and the development of muscle fibers are promoted, and the meat quality is improved.

The invention relates to the related technical field of pig feeds, in particular to a soybean-meal-free feed for increasing the intramuscular fat content of pork and application of the soybean-meal-free feed. The feed comprises the following raw materials: corn, fermented rapeseed meal, wheat bran, rice bran and hull, soybean oil, sodium chloride, calcium hydrophosphate, mountain flour, L-lysine hydrochloride, DL-methionine, L-threonine, L-tryptophan, choline chloride, a trace element premix and a vitamin premix. The feed is suitable for growing-finishing pigs with the weight ranging from 30 kg to the market (the weight ranges from 125 kg to 130 kg). The fermented rapeseed meal contained in the feed disclosed by the invention is produced by treating high-temperature hot-pressed rapeseed meal through a special bacterium-enzyme synergistic fermentation process. Tests prove that the feed does not use soybean meal, but the feed intake, the weight gain, the intramuscular fat content of pork and the economic benefit of pigs are remarkably improved.

The application belongs to the technical field of pig molecular marker, and particularly discloses a SNP molecular marker related to a pig intramuscular fat content character and application thereof. Based on SNP molecular marker, whole genome correlation analysis and meta-analysis are carried out, and two SNP molecular markers related to the pig intramuscular fat content character are screened out. The SNP molecular marker is located on chromosome 5 of the pig, and can be used for marker-assisted selection of the pig intramuscular fat content character.

The invention provides a feed additive and compound feed for improving intramuscular fat of pork, and belongs to the technical field of animal feed. The additive consists of the following components in parts by mass: 30-40 parts of codonopsis pilosula, 20-25 parts of rhizoma atractylodis, 12-16 parts of folium eriobotryae, 10-15 parts of bighead atractylodes rhizome, 10-15 parts of poria cocos, 8-12 parts of hawthorn, 18-22 parts of pomegranate rind and 5-10 parts of liquorice. The feed additive disclosed by the invention can effectively solve the problem that the meat quality and flavor of modern commercial pigs are reduced due to high lean meat percentage breeding through the synergistic effects of tonifying spleen, promoting appetite, promoting nutrient absorption and regulating metabolism. When the feed additive is applied to live pig feeding, the average daily gain and the meat-feed ratio of pigs can be remarkably increased on the premise that the thickness of back fat is not increased, meanwhile, the carcass lean meat percentage, the marbling score and the intramuscular fat content are increased, and the yield and the edible flavor of pork are comprehensively improved.

The invention relates to the technical field of livestock breeding improvement, and discloses a beef cattle high intramuscular fat content prediction method and application. The beef cattle high intramuscular fat content prediction method comprises the following steps: step 1, obtaining a rumen fluid sample of beef cattle to be detected; step 2, detecting the contents of myristic oleic acid, citric acid and salicylate in the rumen fluid sample; and step 3, comparing the content with a preset threshold value, and if the content of at least one metabolite is higher than the corresponding preset threshold value, predicting that the beef cattle has high intramuscular fat deposition potential or high intramuscular fat content. According to the method, early-stage, rapid and accurate prediction of the high intramuscular fat deposition potential of the individual cattle can be realized, the limitation that the traditional method depends on post-slaughter phenotype determination or is long in gene breeding period and high in cost is overcome, and a brand new technical means is provided for early-stage seed selection and precise breeding.

This invention belongs to the field of black pig breeding technology, specifically relating to a method for breeding large-sized, high-quality black pigs. The method involves crossing purebred Songliao black pigs with purebred Berkshire pigs to obtain F1 generation hybrid pigs. The F1 generation hybrid pigs are backcrossed twice with purebred Songliao black pigs to obtain B2 generation hybrid pigs. The B2 generation hybrid pigs are then crossbred to obtain a basic breeding herd of black pigs. This basic breeding herd is then subjected to closed breeding and successive selection for at least six generations to obtain large-sized, high-quality black pigs. The large-sized, high-quality black pigs of this invention have a Berkshire bloodline content of 12.5%, retaining the high fertility and excellent meat quality of Songliao black pigs, while effectively introducing the growth rate and intramuscular fat deposition advantages of Berkshire pigs. The large-sized, high-quality black pigs of this invention have all-black coats, reach 100kg at ≤175 days of age, have a live intramuscular fat content ≥3.5%, and a litter size ≥10.5 piglets, and can be self-breeding.

This invention provides the application of a *Breago brevis* strain in the preparation of products that promote intramuscular fat deposition in livestock and poultry. The *Breago brevis* strain is *Breago brevis* JCM 32280. Under high-fiber dietary conditions, supplementation with *Breago brevis* JCM 32280 of this invention can efficiently degrade fiber to produce high concentrations of butyrate. Butyrate, as a key effector molecule, can directly bind to and activate PPARγ receptors in host cells, thereby upregulating the MOGAT2-mediated triglyceride synthesis pathway, significantly increasing body fat percentage in mammals and promoting intramuscular fat (marbling). Simultaneously, this strain can reshape the intestinal microecology, specifically increasing the abundance of beneficial intestinal lactobacilli and enhancing the intestinal mucus barrier, providing a safe and efficient microbial preparation strategy for improving the fat deposition capacity and meat flavor of livestock and poultry.

The invention discloses an intramuscular fat content prediction method based on a pig ultrasonic image. The intramuscular fat content prediction method comprises the following steps: acquiring the ultrasonic image of a pig; preprocessing the ultrasonic image and obtaining a muscle feature region image; wherein the muscle feature region image is processed to obtain a multi-scale fusion feature map; and performing regression prediction on the output multi-scale fusion feature map to obtain a predicted value of the intramuscular fat content. Compared with the prior art, the method has the advantages that the multi-scale fusion feature map can better represent the distribution features of fat and muscle, the processed result represents the fat content of the muscle more accurately, and the prediction accuracy of the intramuscular fat content is improved.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a pork quality trait gene THRSP, a primer pair and application of the SNP molecular marker and the primer pair, wherein the meat quality trait is intramuscular fat content; the molecular marker is located in a partial sequence of a pig THRSP gene, the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table, and a Ggt exists at the 141bp position of the sequence; and A basic group mutation. The invention also provides a primer pair for amplifying the molecular marker and a method for breeding by using the molecular marker. According to the invention, the correlation between one SNP molecular marker in the pig THRSP gene and the intramuscular fat content character of the pig is explored for the first time, so that the SNP molecular marker can be used for marker-assisted selective breeding of the pig, that is, the new application of the SNP molecular marker in the pig THRSP gene in marker-assisted breeding of the pork quality character is provided, the early screening of the pork quality character is realized, and the screening method is simple and rapid.

The invention discloses a method and device for evaluating intramuscular fat content of a sheep living body based on an ultrasonic image, and relates to the field of intramuscular fat prediction based on deep learning, and the method comprises the steps: obtaining a spinal ultrasonic image of a target sheep, inputting the spinal ultrasonic image into an image segmentation model, and obtaining an eye muscle region segmentation mask in the spinal ultrasonic image; and according to the eye muscle region segmentation mask and the spine ultrasonic image, obtaining an eye muscle region feature map, inputting the eye muscle region feature map into the intramuscular fat content prediction model, and predicting the corresponding intramuscular fat content. On one hand, an image segmentation model adopts an improved model based on a DeepLabV3 + architecture, an eye muscle region can be accurately segmented, and further the intramuscular fat content is accurately predicted, on the other hand, an intramuscular fat content prediction model adopts an architecture based on ConvNext-Tiny, and a global attention mechanism and multi-scale feature fusion are introduced into the architecture, so that the intramuscular fat content prediction accuracy is improved. And the prediction accuracy of the intramuscular fat content of the target sheep is improved.

The invention belongs to the technical field of molecular biology, and relates to miRNA for regulating and controlling the content of fat and / or unsaturated fatty acid in buffalo muscle and application of the miRNA. The miRNA is miR-30f (bta-miR-30f), and the fat deposition in the buffalo muscle and the composition of unsaturated fatty acid are influenced by regulating and controlling the expression of an RAD23B gene. The invention finds that transfection of a bta-miR-30f simulant in buffalo intramuscular fat cells can significantly improve intramuscular fat content and unsaturated fatty acid proportion, while transfection of an inhibitor shows a reverse effect. Further research finds that knock-down of the RAD23B gene can also increase the content of triglyceride and unsaturated fatty acid in buffalo muscle tissue. Important gene resources and molecular breeding technical means are provided for genetic improvement of buffalo meat quality characters (including flavor characteristics and nutritional value).