184 results about "Intramuscular fat" patented technology

The invention relates to a molecular marker associated with pork intramuscular fat and application. The SNP is located at a single nucleotide Ggt occurring at the 6114468bp position of a first chromosome of a pig genome; the A polymorphic site is shown in the specification. A target sequence containing the mononucleotide mutation site is subjected to PCR (Polymerase Chain Reaction) amplification by utilizing a primer, and the PRKN genotype can be quickly identified by sequencing an amplification product, so that the difference of pork quality is detected, and guidance is provided for breeding of pig varieties.

The invention discloses a method for improving beef quality, an action mechanism and an experimental method, in a cattle body, vitamin A can activate the expression of EBF2 through an active metabolite RA of the vitamin A, and the EBF2 can inhibit the transcription process of CYP26B1 in a targeted manner to maintain the activity of a retinol signal channel, so that PPAR gamma and downstream lipid metabolism related genes thereof are activated, and the activity of the retinol signal channel is improved. Fatty acid transport and lipid accumulation in fat cells in the cattle muscle are promoted, fat deposition in the cattle muscle is promoted, and the beef quality is improved. By constructing the molecular network for regulating and controlling the formation of the fat in the cattle muscle, a complex regulation and control mechanism for controlling the fat deposition in the cattle muscle can be understood more deeply, a method for improving the beef quality based on regulating and controlling the vitamin A mediated key factor EBF2 is provided, and a new way is provided for improving the beef quality in a targeted manner.

The invention belongs to the field of agricultural automation and intelligent equipment, and discloses a portable measuring method and device for pork intramuscular fat content. By screening the characteristic wave bands related to the fat content and selecting the corresponding narrow-band LEDs, the accuracy and effectiveness of the multispectral image are improved. And meanwhile, through multi-modal image fusion analysis, in combination with the RGB image, the black-and-white image and the multi-spectral image, the precision of target segmentation and content prediction is improved. And establishing an end-to-end content prediction model by adopting a mode of combining a CNN and an MLP network. Meanwhile, the FCNN network is used for processing the spectral data, the tedious spectral data preprocessing process is omitted, the advantages of the spectral data and the RGB image are fully played, and a new thought and method are provided for the field of spectral modeling. The technical scheme has the advantages of portability, high efficiency, accuracy and the like, provides powerful support for pork nutrition evaluation, and can provide reference for determination of the fat content of other livestock and poultry meat.

The invention relates to a biological preparation for improving quality of weever, the biological preparation comprises an injection and an edible agent, the injection comprises chemically modified chemically synthesized single-stranded small RNA, Rev-erbalpha protein and Per1 protein, and the edible agent comprises Clock core peptide 1, Clock core peptide 2 and taurine. Skeletal muscle development, liver lipid metabolism and generation of cell inflammatory factors of the weever are influenced by adding and injecting the feed, so that the quality of the weever is improved, intramuscular fat deposition is promoted, the taste and flavor of fish meat are improved, the energy reserve of the weever can be improved, and the culture cycle is shortened. The improvement of cell inflammatory factors shows that the disease resistance of the weever is improved, the survival rate in the breeding process is further improved, and the use amount of antibiotics is reduced. The method provides an important theoretical basis for improving fish meat quality in China and promoting high-quality, efficient and healthy development of aquaculture, and has a wide market prospect.

The invention belongs to the technical field of molecular biotechnology and molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) site related to pig intramuscular fat content, a related molecular marker and application of the SNP site. The SNP site corresponds to the 151330919bp site of a No.6 chromosome of an international pig reference genome version 11.1, the polymorphism of a base of the site affects the intramuscular fat content of the pig, the related index of a TT genotype is greater than that of a pig individual with TG and GG genotypes, and the related index of the TG genotype is greater than that of a pig individual with GG genotype. According to the invention, a molecular breeding means is utilized to solve the problem of improving the character expression of the intramuscular fat content of the pig, and by optimizing the dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the intramuscular fat content of the pig can be improved, the pork quality can be improved, the genetic modification breeding of the pig can be accelerated, and the economic benefit of a pig farm can be improved.

The invention provides a Jinhua pork quality improvement method and system based on data mining, and relates to the technical field of intelligent breeding, and the method comprises the steps: obtaining an original data set; performing data fusion according to the original data set, and obtaining a multi-modal association graph by constructing an entity-relation network topology structure; performing potential influence path extraction according to the multi-modal association map to obtain an influence path set; performing interaction effect modeling according to the influence path set to obtain a dynamic effect model; performing meat defect tracing according to the dynamic action model to obtain a defect driving factor set; and generating an optimization strategy according to the defect driving factor set to obtain a meat quality improvement decision scheme. According to the method, the multi-factor relation network is constructed by integrating Jinhua pig full-period multi-source data, the key conduction path and the dynamic interaction model influencing meat quality formation are accurately extracted, and the nonlinear antagonism relation between the leaf fat deposition tendency and intramuscular fat accumulation under the specific physiological mechanism of the Jinhua pigs is systematically quantified.

The invention discloses a method for producing snowflake beef through yak seasonal barn feeding and short-term fattening. Belongs to the field of animal husbandry, and achieves efficient output of yak snowflake beef through short-period seasonal fattening by selecting fattening yaks, selecting fattening seasons and time and cooperating with daily ration in the fattening early stage and the fattening later stage. In the late fattening period, soybean-meal-free low-protein daily ration is adopted to synergistically promote fat deposition in yak muscles, so that efficient output of the yak snowflake beef is achieved, and the method for producing the snowflake beef by using the yaks has important significance for improving the economic benefits of yak breeding in plateau pasturing areas and meeting the requirements of the market in China for high-quality beef.

The invention provides a cattle FTO gene promoter mutation site and application thereof, and belongs to the field of biotechnology and genetic engineering, the mutation site is located in an FTO gene promoter region on a cattle genome 18 # chromosome, and the mutation site is specifically characterized in that an A base at a transcriptional start site upstream-33 base position is mutated into a G base (chromosome coordinate: Chr: 22040490); according to the mutation, the expression level of the FTO gene is remarkably improved, and the differentiation capacity of intramuscular fat precursor cells is remarkably enhanced; the FTO gene promoter mutation site provided by the invention not only can increase the gene expression quantity, but also can promote the differentiation of intramuscular fat precursor cells, and provides a solid technical basis for the cultivation of new beef cattle varieties and the increase of intramuscular fat content.

The invention relates to the technical field of pig breeding, in particular to an SNP molecular marker for identifying pork quality, a primer and application of the SNP molecular marker, the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, N at the 140th site of the sequence is an SNP site, and the polymorphism is T or C; the unfreezing water loss rate and intramuscular fat content of the pork with the CC genotype are higher than those of the pork with the TT genotype and CT genotype, and the molecular marker can be used for detecting the difference of pork quality and is applied to continuous breeding of the Songhua black pigs and early breeding in production.

The invention discloses a method and a device for predicting, regulating and controlling intramuscular fat of broiler chickens based on deep learning. The method comprises the following steps: acquiring broiler chicken growth condition data including temperature, humidity, food intake and exercise amount; a TCN-MAMBA fusion model is constructed through a time convolution network TCN and an MAMBA model; the method comprises the following steps of: improving a dung beetle optimization algorithm DBO by adopting Piecewise chaotic mapping and an eagle algorithm OOA, and optimizing hyper-parameters of a TCN-MAMBA joint model by utilizing the improved ODBO; establishing a broiler growth prediction model, and taking the broiler growth condition parameters as input of the ODBO-TCN-MAMBA prediction model to obtain a broiler growth period prediction result; based on the established broiler growth prediction model, optimal broiler growth condition parameters are generated through an ODBO algorithm, and the broiler growth environment is accurately adjusted. Compared with the prior art, the broiler chicken growth conditions can be accurately regulated and controlled, broiler chicken intermuscular fat is controlled to be in the optimal state, and the flavor and taste of broiler chicken meat are improved.

The invention belongs to the technical field of animal breeding, and particularly relates to a molecular marker closely linked with beef quality characters, a primer pair, a detection reagent and application. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, the nucleotide shown as SEQ ID NO.1 has G / A polymorphism at the 831th bp, when the genotype of the 831th site from the 5'end of the molecular marker is GG, the meat color brightness and intramuscular fat content of an individual are higher than those of a GA genotype individual, and the cooking loss of the individual is lower than that of the GA genotype individual, and the molecular marker can be used for selective breeding of high-quality beef cattle.

The invention discloses a molecular marker related to pig intramuscular fat content and meat color character and application, and belongs to the technical field of molecular markers. A section of 2279bp insertion structure variation exists in a ninth exon region of the FMO3 gene, and polymorphism detection in a Beijing black pig population finds that three types of genotypes, namely homozygote insertion (AA), homozygote insertion-free (WW) and heterozygote (AW), exist on the locus. The three genotypes and Beijing black pig meat quality characters are subjected to correlation analysis, and the result shows that the intramuscular fat content of the WW genotype is remarkably higher than that of the AA genotype and the AW genotype; the meat color character of the AA genotype is obviously higher than that of the AW genotype. The invention provides a new thought for further researching the effect of the FMO3 gene on the pork quality character, and provides a theoretical reference for the breeding work related to the pork quality.

The invention relates to the technical field of pigs, in particular to a molecular marker associated with pork color and intramuscular fat content and application. The molecular marker is an SNP (Single Nucleotide Polymorphism) site of nucleotide at a position 7314350 of a chromosome 1 NC010443.5 of a pig reference genome GCF 000003025.6. The molecular marker is a molecular marker of a pig genome GCF 000003025.6 The molecular marker is closely associated with pork color and intramuscular fat content characters. The molecular marker can be applied to molecular-assisted selective breeding of high-quality pork quality characters.

The invention discloses lactobacillus plantarum YBE2 and application thereof.According to the lactobacillus plantarum YBE2 for high yield of extracellular polysaccharide mainly containing rhamnose, different cholate types can be degraded at the same time, glycocholic acid is specifically hydrolyzed, and the lactobacillus plantarum YBE2 has the effects of lowering cholesterol and relieving acute inflammation at the same time. Meanwhile, the plant lactobacillus YBE2 can reduce the deposition of fat in the belly of the cage rearing broiler chicken, improve intramuscular fat, promote the deposition of fatty acid in muscles, reduce the total bile acid of plasma, triglyceride and low-density lipoprotein cholesterol, and remarkably improve the lipid metabolism and muscle quality of meat poultry, and can be widely applied to the breeding of the cage rearing broiler chicken, promote the healthy breeding of the meat poultry and improve the quality of the meat poultry. The culture benefit is improved.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to low backfat thickness and high intramuscular fat content of a pig on an IL-15 (Interleukin-15) gene and application of the SNP molecular marker, and belongs to the field of molecular biology. According to the invention, an SNP site is screened, and the SNP site is located at the upstream 334bp of an initiation codon of an IL-15 gene, specifically G / A mutation. The research finds that the genotype of the SNP site is closely related to the backfat thickness and intramuscular fat content at the same time; the intramuscular fat content of GG genotype and GA genotype individuals is remarkably higher than that of AA genotype individuals, and the backfat thickness of the GG genotype individuals is remarkably lower than that of the AA genotype individuals. The invention provides an important molecular marker for the improvement and breeding of high-yield and high-quality pig breeds by using the SNP of the IL-15 gene, and provides certain help for the research of pig carcass traits and meat quality traits.

The invention discloses an SNP molecular marker related to a pork quality character gene NRG2, a primer pair and application of the SNP molecular marker and the primer pair, and relates to the technical field of pig molecular markers. Wherein the meat quality character is an intramuscular fat content character; the molecular marker is located in a first intron of an NRG2 gene of a second chromosome of a pig, the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table, and g.86Cgt exists at the 86bp position of the sequence; the T basic group is mutated. The invention also provides a primer pair for amplifying the molecular marker and a method for breeding by using the molecular marker. According to the invention, the correlation between one SNP molecular marker in the pig NRG2 gene and the intramuscular fat content character of the pig is explored for the first time, so that the SNP molecular marker can be used for marker-assisted selective breeding of the pig, namely a new application of the SNP molecular marker in the pig NRG2 gene in marker-assisted breeding of the pork quality character is provided, the early screening of the breeding character of the pig is realized, and the breeding efficiency of the pig is improved. The screening method is simple and rapid.

The invention belongs to the technical field of animal breeding, and particularly relates to application of a detection reagent of an SNP (Single Nucleotide Polymorphism) molecular marker related to pork quality traits. Basic groups at 208bp and 217bp from the 5'end of the sequence have synchronous C / T mutation, the sequence shows three genotypes of CC, CT or TT, T allele is dominant allele, and the intramuscular fat content of the TT type pig is greater than that of the CC type pig. The invention further develops a reagent for detecting the SNP molecular marker, the reagent can be used for pig assisted breeding, pig breeding, pork quality character identification or pork quality character screening, and a method for identifying the intramuscular fat content of pork and screening pork with rich intramuscular fat is developed.

The present invention discloses the application of the MYLK gene and the CPSF2 gene in regulating beef intramuscular fat deposition, belonging to the field of genetic engineering technology. By studying the APA mechanism during cattle intramuscular fat deposition, the present invention found that during the IMF deposition process, the 3′UTR of the MYLK gene shortened and the expression level of the MYLK gene increased. During the shortening process, the miRNA involved in IMF deposition was lost, thereby regulating the reduction of IMF deposition; after the 3′UTR of the CPSF2 gene was shortened, its expression was also upregulated, regulating the reduction of IMF deposition. The research results of the present invention provide new theoretical support and technical guidance for the prediction of beef quality and the breeding of high-quality beef cattle, which is of great significance to promoting the development of animal husbandry and improving the production efficiency of animal husbandry.

The invention discloses a live pig intramuscular fat detection method and device based on multiple modes. The method comprises the following steps: acquiring growth data of a pig and a plurality of B ultrasonic image data of a preset part, and encoding the growth data to obtain a first feature; inputting the B-mode ultrasound image data into a pre-trained intramuscular fat region detection network in sequence, and obtaining an intramuscular fat region image of each B-mode ultrasound image; and extracting a second feature from all the intramuscular fat region images, splicing the second feature with the first feature, inputting the spliced second feature into a pre-trained multi-layer perceptron network, and obtaining an intramuscular fat detection result of the pig. Through the application, the equipment cost of pig intramuscular fat detection can be reduced, the flexibility of pig intramuscular fat detection is improved, and the accuracy of pig intramuscular fat detection is further improved through multi-modal data composed of the growth data and the B ultrasonic image data.

The invention relates to the technical field of molecular biology, and discloses a miRNA molecule for regulating and controlling fat deposition in pig muscle, one of the purposes of the invention is to provide a miRNA molecule for regulating and controlling fat deposition in pig muscle, the miRNA is SSC-miR-874, and the mature sequence of the miRNA is shown as SEQ ID NO1: CUGCCCUGGCCGAGGGACCGAC. The second purpose of the invention is to provide a product, and the product comprises SSC-miR-874 mimics or SSC-miR-874 inhibitor, and is characterized in that the SSC-miR-874 mimics and the SSC-miR-874 inhibitor are added into the product; wherein the sequences of the SSC-miR-874 mimics are as shown in SEQ ID NO1 (sequence identifier number 1) and SEQ ID NO2 (sequence identifier number 2), and the sequence of the SSC-miR-874 inhibitor is as shown in SEQ ID NO3 (sequence identifier number 3). The application reveals that the SSC-miR-874 can negatively regulate and control the proliferation and adipogenic differentiation of the precursor fat cells in the pig muscle, provides a new molecular target for deeply understanding the fat deposition mechanism in the pig muscle, provides an effective miRNA intervention strategy for optimizing the pork quality and increasing the fat content in the muscle, and has important breeding application value and industrialization prospect.

The invention provides a sheep intramuscular fat content estimation method and system based on an ultrasonic image. The method comprises the following steps: acquiring the ultrasonic image; performing target area segmentation and marking on the ultrasonic image to obtain a target area mask; splicing the ultrasonic image with the target area mask to obtain a feature image; extracting image features of the feature image through a convolutional neural network; and on the basis of the image features, predicting through an MLP model to obtain an intramuscular fat content value. The IMF content can be objectively analyzed, and the accuracy and reliability of IMF content prediction are improved.

The invention discloses a method for predicting intramuscular fat of a living body, a processing device and a computer readable storage medium. The method comprises the following steps: acquiring a target image of the living body by utilizing ultrasonic equipment; inputting the target image into a first model to determine the quality of the target image; and if the quality of the target image is qualified, inputting the target image into a second model to predict the intramuscular fat content of the living body. Through the scheme, accurate prediction of intramuscular fat of a living body can be realized.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to intramuscular fat content characters of Rongchang pigs, a detection primer and application, and belongs to the technical field of molecular biology. The invention develops an SNP marker related to the intramuscular fat content character of Rongchang pigs and a detection primer based on an SNP marker technology. The SNP marker developed by the invention is located at the 121626638bp position on the No.7 chromosome of the Rongchang pig and relates to C / T mutation, and when the genotype is CT, the intramuscular fat content of the Rongchang pig is obviously higher than that of the TT genotype. Individuals with excellent intramuscular fat content can be screened out through the SNP marker, so that the level of the intramuscular fat content character of the Rongchang pigs is improved, and the breeding process of new varieties is accelerated.

The present disclosure relates generally to methods and test kits for identifying SNPs associated with desirable meat eating quality traits in ovine animals. In particular, the present disclosure relates to a SNP-based diagnostic test and method for identifying ovine animals with desirable fat melting point (FMP), intramuscular fat (IMF) and omega-3 long chain polyunsaturated fatty acids (n-3 LC PUFAs) which are characteristic of Australian White sheep or Lamb (e.g., Tattykeel Australian White Lamb), and the use of those tests and methods in animal breeding programs.

The invention belongs to the technical field of animal breeding, and particularly relates to a molecular marker closely linked with beef intramuscular fat traits, a primer pair, a kit and application. Nucleotide sequences of the molecular marker are shown as SEQ ID NO.1 and SEQ ID NO.2, the molecular marker has T / C polymorphism from the 76th basic group of the 5'end, and when the genotype of the locus is TC, the intramuscular fat content of beef of an individual is higher than that of the individual with the genotype of TT. The beef cattle with the high intramuscular fat content character can be screened or cultivated by utilizing the molecular marker.

The invention relates to the technical field of pigs, in particular to a molecular marker related to pig carcass length and intramuscular fat content characters and application. The molecular marker is an SNP (Single Nucleotide Polymorphism) site of nucleotide at a position 7311198 of a chromosome 1 NC010443.5 of a pig reference genome GCF 000003025.6. The molecular marker has the advantages that the molecular marker can be applied to pig breeding; the molecular marker is closely associated with the characteristics of pig carcass length and intramuscular fat content. The molecular marker can be applied to molecular-assisted selective breeding of high-quality pig carcass and meat quality traits.

The invention discloses a multi-mode-based sheep non-injury intramuscular fat content detection method, system and device and a medium, and relates to the field of intramuscular fat evaluation.The method comprises the steps that an ultrasonic image of to-be-detected sheep is obtained; extracting physiological attribute characteristics of the sheep to be detected based on the ultrasonic image; a DB-KAN dual-branch network is constructed and trained; the DB-KAN double-branch network comprises a CNN (Convolutional Neural Network) branch, a Transform branch and a regression head based on the KAN; and inputting the ultrasonic image and the physiological attribute characteristics into a trained DB-KAN double-branch network to obtain the intramuscular fat content of the sheep to be detected. The intramuscular fat content can be rapidly and accurately detected in a non-invasive manner.

The invention discloses a yak intramuscular precursor fat immortalized cell line and a preparation method thereof. The method comprises the following steps: (1) obtaining yak primary intramuscular precursor fat cells; (2) performing SV40T lentivirus infection on the precursor fat cells in the yak muscle and screening cell strains: performing SV40T lentivirus infection by utilizing a 1 / 2 small-volume infection method to obtain immortalized cells after the precursor fat cells in the yak primary muscle are infected with SV40T, namely immortalized cells for short; puromycin is adopted to screen immortalized cells; and diluting the screened cells by using a limited dilution method, and carrying out enlarged culture to obtain the immortalized cell line. The yak intramuscular precursor fat immortalized cell line constructed by the invention has genetic homogeneity, the heterogeneity influence of cell populations is eliminated, and an in-vitro cell model is provided for subsequent research related to yak intramuscular fat cells.

The application discloses a SNP marker related to an intramuscular fat content character of Rongchang pigs, a detection primer and application, and belongs to the technical field of molecular biology. The application develops a SNP marker and a detection primer related to the intramuscular fat content character of Rongchang pigs based on a SNP marker technology. The SNP marker developed by the application is located at 121626638 bp of a No. 7 chromosome of Rongchang pigs, and relates to C / T mutation. When the genotype is CT, the intramuscular fat content of Rongchang pigs is significantly higher than that of the TT genotype. The SNP marker can screen out individuals with excellent intramuscular fat content, so that the level of the intramuscular fat content character of Rongchang pigs is improved, and the breeding process of new breeds is accelerated.

The application provides a method for optimizing the slaughter weight of Jinfen white pigs to produce high-quality flavor pork. Through systematic research on the influence of different slaughter weights on the pork quality and flavor of Jinfen white pigs, it is first determined that when the slaughter weight is 135.08±1.10 kg, the pork has the optimal comprehensive quality performance, which is specifically embodied in that the intramuscular fat content reaches (3.82±0.05)%, the shear force is (35.75±1.68) N, and the meat quality is excellent. At the same time, the pork of the weight group has the most total volatile substances, and the types of various volatile substances are relatively rich, and exhibits the best flavor characteristics. The application not only provides clear process parameters for the production of high-quality flavor pork of Jinfen white pigs, but also provides a reliable technical basis for the precise regulation of meat quality based on the slaughter weight.