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Primer pair for SNP marker related to content of intramuscular fat of Suhuai pig and application thereof

A Suhuai and pig muscle technology, applied in the field of SNP marker primer pairs, can solve the problem of inability to determine the main gene and its key mutation sites.

Inactive Publication Date: 2018-12-07
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] According to the porcine QTL database website (http: / / www.animalgenome.org / cgi-bin / QTLdb / SS / index), there is currently no QTL that affects intramuscular fat content on chromosome 16 in pigs, and other chromosomes All QTLs affecting intramuscular fat have been located, but most of them are QTLs located by microsatellite markers, and the confidence interval is mostly 10-20cM. It is impossible to determine the real main effect genes and their key mutation sites, so it is difficult to directly apply Pig Breeding Improvement

Method used

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  • Primer pair for SNP marker related to content of intramuscular fat of Suhuai pig and application thereof
  • Primer pair for SNP marker related to content of intramuscular fat of Suhuai pig and application thereof
  • Primer pair for SNP marker related to content of intramuscular fat of Suhuai pig and application thereof

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Embodiment 1

[0026] 1 Source of test animals

[0027] Huaiyin Pig Farm in Huai'an City, Jiangsu Province

[0028] 2 Extraction of Suhuai Pig Genomic DNA

[0029] 4 tissue samples were collected from 314 Suhuai pigs, one of which was used for individual DNA extraction;

[0030] Refer to the instructions of Tiangen Biotechnology Company Tissue DNA Extraction Kit, the extraction is performed in the following order:

[0031] ①Add 68mL and 200mL absolute ethanol to buffer GD and rinse solution PW respectively, and mix well.

[0032] ②Collect about 100 mg of tissue sample and place it in a 2 mL EP tube, cut it into pieces, add 200 μL of buffer GA, and oscillate until completely suspended.

[0033] ③ Add 20 μL of proteinase K solution, mix well, and place in a 56°C metal bath to digest overnight until the ear-like tissue dissolves, and briefly centrifuge to remove water droplets on the inner wall of the tube cap.

[0034] ④ Add 200 μL buffer GB, mix thoroughly by inversion, put in a metal bat...

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Abstract

The invention relates to a primer pair for an SNP marker related to content of intramuscular fat of a Suhuai pig and an application thereof. The SNP marker is located on a nucleotide sequence of an SCD gene on a chromosome 14 of the pig; a site of the SNP marker is a molecular marker of a rs80912566 nucleotide site contained in the chromosome 14 of the pig in a reference sequence of an international pig genome 11.1 version, and the rs80912566 nucleotide site has C / T polymorphism; and the SNP marker is significantly correlated with the content of the intramuscular fat of the Suhuai pig (P<0.05). and the primer pair is used for detecting the SNP marker, an upstream primer is SEQ ID NO:2, and a downstream primer is SEQ ID NO:3. The SNP marker is related to the intramuscular fat of the Suhuaipig; the SNP marker can be used to screen Suhuai pig strains with high intramuscular fat; and the obtained Suhuai pig strains with the high intramuscular fat has important economic and social values.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a pair of SNP marker primers related to intramuscular fat content of Suhuai pigs and an application thereof. Background technique [0002] Intramuscular fat (IMF) is an important indicator affecting meat quality. Studies have shown that intramuscular fat content is positively correlated with meat quality-related traits (extensibility, juiciness, taste, etc.). Pork is one of the main meats on people's table. Statistics show that in 2016, China's annual pork consumption ranked first in the world. It is evident that people love pork. Since the 1980s, in order to meet the demand for pork consumption in the domestic market, various places have widely promoted the use of Du long hybrid combinations to produce commercial lean meat pigs, such as Duroc pigs, Landrace pigs, and Large White pigs. However, while pursuing high yield, it has also led to a serious decline in pork qu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6806C12Q1/6888C12Q1/6869C12N15/11
CPCC12Q1/6806C12Q1/6869C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113
Inventor 黄瑞华王彬彬李平华周五朵蒲广王欢兰亭旭张倩高琛牛培培张总平李强
Owner NANJING AGRICULTURAL UNIVERSITY
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