137 results about "Large white" patented technology

The invention discloses an SNP (single-nucleotide polymorphism) marker set for identifying pig breeds. The application method mainly comprises the following steps: by using 200 purebred Large White and Landrace individuals as study objects, carrying out genome SNP 60K chip scanning typing on all the samples of the two breeds; after carrying out quality control on all the data according to the requirements, screening out SNP marker sites capable of identifying Landrace and Large White, and carrying out genome correlation analysis to screen out 16 SNP sites for identifying Landrace and Large White; and carrying out verification on another 500 individuals by principal component analysis and cluster analysis, wherein the accuracy of the 16 SNP sites reaches 99%, which indicates that the 16 SNP sites can be used for identifying Large White and Landrace.

The invention discloses a white balance adjusting method, belongs to the technical field of white balance adjustment, and aims at solving the problems of large white point selection error, easy missing and accurate selection. The method comprises the steps of (1) acquiring a Bayer template RGB interpolation image, and converting the RGB image into a YCbCb image; (2) dividing the YCbCb image into a plurality of brightness level areas in a YCbCb space according to a brightness component Y, and counting Y, Cb and Cr components; (3) counting the statistic value of the candidate white point in each brightness level area and the quantity of the candidate white points falling in the area according to the counted Y, Cb and Cr components; (4) selecting white points from the top brightness level area by the double-threshold method; (5) acquiring average number of YCbCb through the selected white points; (6) converting the acquired YCbCb average number into R, G and B average values of the RGB space; (7) obtaining the channel gain for white balance adjustment according to the R, G and B average values so as to achieve the white balance processing of a colored image. With the adoption of the method, the stability and accuracy of statistics of the white points can be improved.

The invention discloses a structural variation 177 (SV177) for distinguishing varieties of large white pigs and Chinese indigenous pigs. The SV177 is characterized in that the SV177 is positioned at chrX: 100561156-100562159 of a pig reference genome Sscrofa 10.2, missing genes of the SV177 are determined as D, and normal genes which are not missed are determined as A. When a detection technology provided by the invention is used for detecting the SV177 genotype of a sample, the large white pigs have three genotypes and give priority to the genes A which are not missed, and the Chinese indigenous pigs are free from a heterozygous genotype (DA) and give priority to the missing genes D; therefore, the SV177 can be used for distinguishing the varieties of the large white pigs and the Chinese indigenous pigs as a molecular marker.

The invention belongs to the technical field of porcine molecular marker screening, and in particular relates to porcine NTF3 promoter region SNP as a molecular marker for the boar breeding traits and applications of the porcine NTF3 promoter region SNP. The molecular marker is cloned from the 5' flank promoter fragment of the porcine NTF3 gene, the nucleotide sequences of the molecular marker are shown as SEQ ID NO:1 and SEQ NO:2, allele mutation of A/G and G/A exists at the 92nd basic group site of the sequence shown as SEQ ID NO:1 and SEQ NO:2, and the allele mutation causes the polymorphism of MvaI-RFLP. The marker is utilized for carrying out correlation analysis on the boar breeding traits of large white pigs and duroc pigs. The breeding traits comprise the boar sperm motility and the sperm density trait. The invention further discloses a typing detection method of the marker, and therefore, a novel marker is provided for selecting the boar breeding traits. The marker can be used for evaluating the boar breeding traits and researching the genetic improvement on the breeding boar breeding performance.

The invention aims to provide a detection method for searching the genetic mutation sites of porcine MMP23 and the genetic polymorphism, namely, the molecular marking method for speculating and identifying the litter size with MMP23. The invention provides the sequence of genomic nucleotides with a length of 482bp including the exons 2, 3 and 4 and the introns 2 and 3 of the porcine MMP23 gene of large white sows, Meishan pigs and Qingping pigs, and provides the single nucleotide polymorphism (SNP) of at two sites situating in the interior of the proliferated 482bp-long segment. The method provided by the invention can be used for developing diagnostic procedures or kits used for selecting the pigs carrying the advantageous alleles in breeding programs with better selective outcome.

The invention discloses a molecular marker SV193 for distinguishing Chinese local pig breeds from Large White pigs and an application technology of the molecular marker SV193. The molecular marker SV193 is characterized in that SV193 is located at chr8:142093174-142093446 of a pig reference genome Sscrofa 10.2, the deleted gene of SV193 is set to be D, and the normal undeleted gene is set to be A. With the application of the detection technology, the results that the Large White pigs mainly rely on the undeleted gene A while the Chinese local pig breeds mainly rely on the deleted gene D are obtained, and the Large White pigs can be distinguished from the Chinese local pig breeds by the application of the molecular marker SV193.

The invention belongs to the field of molecular biological technologies and molecular marker technologies, and particularly relates to an SNP (single nucleotide polymorphism) molecular marker with influence on birth weight characters of pigs and application of the SNP molecular marker. SNP loci of the SNP molecular marker with the influence on the birth weight characters of the pigs correspond tomutation of 138797629th nucleotide loci T C on eighth chromosomes in 10.2 versions of international pig reference genomes and are named g138797629 T C. The invention further provides a primer for identifying the SNP molecular marker. The SNP molecular marker, the application and the primer have the advantages that efficient and accurate molecular marker-assisted breeding technologies can be established by the aid of the SNP molecular marker and the primer, dominant allelomorphic genes of the SNP molecular marker can be optimized, genetic progress of the birth weight characters of the large white pigs can be improved, the survival rate of piglets can be increased, the breeding time for the birth weight characters of the large white pigs can be shortened, accordingly, the breeding pig breeding economic benefits can be effectively increased, the production costs of enterprises can be reduced, and the core competitiveness can be improved.

The invention belongs to the fields of a molecular biotechnology and a molecule marker technology, and particularly relates to application of a pig SNP molecular marker in pig daily gain characters study and/or pig breeding. A pig SNP molecular marker loci is as shown in SEQ ID NO.1, and the 321st nucleic acid single-base located at a sequence segment is mutated and is named as g15588826C>T. An SNP molecular marker is mutated corresponding to the 15588826th nucleotide site C>T on an international pig reference genome 10.2 version chromosome 3. According to the invention, through optimizing a predominant allele of the SNP molecular marker, the genetic progress of daily gain characters of large white can be increased, and the breeding time of the daily gain characters of the large white can be reduced, so that the economic benefit of pig breeding is effectively improved.

The invention relates to a method for breeding lean swine, and belongs to the field of genetic breeding of animals. Suzhong swine of series 1 and 2 is taken as a female parent, famous foreign British large white swine with high growth speed and high lean yield is taken as a male parent, and the lean swine is hybridized and synthesized by using a modern genetic breeding theory and an advanced breeding means. Breeding standards comprise white whole-body clothing hair, a littering size of more than 13, daily gain of 810g and carcass lean yield of over 60 percent. Compared with the Suzhong swine of the series 1 and 2, the growth speed, lean yield and the like of the bred lean swine are remarkably improved.

The invention discloses neodymium iron boron magnetic chalk. A formula of the chalk is as follows: 3-5 parts of neodymium iron boron magnetic material powder, 15-20 parts of burnt gypsum powder, 15-20 parts of large white powder, 15-20 parts of water, and 3-5 parts of color paste, wherein the color paste is an aqueous solution comprising 2-5 parts of polyvinyl acetate emulsion, 2-3 parts of polyvinyl alcohol and 50 parts of water. The chalk is matched with an iron blackboard for use, and has the advantages of fluent writing, clear handwriting, moderate hardness and no dust rise.

The invention discloses a method for identifying pig backfat thickness based on rs80995809 locus genotyping and its application. The method is used to detect whether the genotype of the rs80995809 locus in a pig individual is a GG genotype, a TT genotype or a GT genotype, according to the genotype of individual pigs, the backfat thickness of pigs is determined: the backfat thickness of the pigs with GG genotype is thicker than the backfat thickness of the pigs with GT genotype, and the backfat thickness of the pigs with GT genotype is greater than the backfat thickness of the pigs with TT genotype. The experiment proves that the rs80995809 locus has a significant effect on the pig backfat thickness, the selected SNP can be used as a molecular genetic marker, the numbers of trait loci thatare related or closely linked to the backfat thickness can be looked for, which can directly perform genotype selection or marker-assisted selection for large white pigs, and the breeding of dominantvarieties of the large white pigs can be accelerated.

The invention discloses a breeding method for producing high-quality synthetic-line commercial pigs by utilizing Luchuan pigs. The breeding method includes that male parents selected from large white pigs hybridize with female parents which are also selected from the large white pigs, and high-quality large white male pigs are selected from the produced hybrid generations to serve as final male parent breeds; the local Luchuan pigs are selected as female parents of the synthetic-line pig parent generation and hybridize with landrace serving as the male parents of the synthetic-line pig parent generation, and high-quality female Changlu pigs are selected from the produced hybrid generations to serve as female parent breeds; the selected male parents and the selected female parents are subjected to hybridization, and the hybrid generations serve as the high-quality synthetic-line commercial pigs which are then put into production. The breeding method for producing the high-quality commercial-line commercial pigs by utilizing the Luchuan pigs has the advantages that the high-quality local pig breeds, namely the Luchuan pigs, of Guangxi are utilized for breeding a special strain of the Luchuan pigs, and the large white pigs, the landrace and the Luchuan pigs are subjected to three way hybridization to form a complete set for producing high-quality commercial pork pigs, wherein the Luchuan pigs serve as the female parents in the synthetic-line pig parent generation, the landrace serves as the male parents in the synthetic-line pig parent generation, the Changlu female parent pigs are obtained by means of hybridization, and the large white pigs serving as the synthetic-line final male parents hybridize with the Chuanglu female parent pigs so as to produce high-quality Landrace Luchuan commercial pigs.

The invention discloses an SNP molecular marker relevant to growth traits of a large white pig and application of the SNP molecular marker. The invention belongs to the molecular marker breeding technical field. The SNP molecular marker is located on the locus rs701332503 of the gene DHRS4 of the large white pig. The nucleotide sequence of the gene DHRS4 in the large white pig is relevant to the growth traits of the large white pig from the 5' end locus rs701332503, that is to say, the SNP molecular marker on the locus rs701332503 of the gene DHRS4 of the large white pig is relevant to the growth traits like the feed conversion ratio of the large white pig and is a new molecular marker, by determining the genotype of the SNP locus of the to-be-tested large white pig, the growth traits likethe feed conversion ratio of the large white pig are subjected to breeding pig sifting, the production cost can be effectively reduced, genetic progress can be accelerated, sifting of large white breeding pigs is better served, the huge market requirement can be met, and the SNP molecular marker also has high economic application value and scientific research value.

The invention relates to the technical field of hybrid breeding, and discloses a hybrid breeding method for increasing fat content in pig muscle. The specific technical solution is to select Xinmei Duroc (EB5) as the male parent, and Large White, Landrace or both hybrid pigs as the female. Originally, crossbreeding was carried out, and the first-generation hybrid sows (50% EB5) were selected and then crossed with Xinmei Duroc (EB5) boars. The offspring produced were commercial pigs (75% EB5) with high intramuscular fat content. The offspring are fed with targeted feed for fattening. The intramuscular fat content is not significantly different from that of Xinmei Duroc, but the growth cycle is shorter than that of Xinmei Duroc, the feed remuneration is high, and the meat quality and flavor are unique. The breeding procedure of the invention is simple, the economic benefit is improved, the breeding cost is reduced, and the utilization and popularization are convenient.

A SNP marker affecting pig daily gain traits and its application. The invention belongs to the field of molecular biotechnology and molecular marker technology, and particularly relates to the application of a porcine SNP molecular marker in the study of pig daily gain traits and pig breeding. The porcine SNP molecular marker site is shown in SEQ ID NO.1, located in the 321st nucleic acid single base mutation of the sequence fragment, named: g15588826C>T. The SNP molecular marker corresponds to the C>T mutation at the 15588826th nucleotide site on chromosome 3 of the International Porcine Reference Genome Version 10.2. By optimizing the dominant allele of the SNP molecular marker, the invention can increase the genetic progress of the daily gain trait of the Large White, reduce the breeding time for the daily gain trait of the Large White, thereby effectively improving the economic benefits of breeding pigs.

The invention provides a method and apparatus for extracting a target image, and relates to the technical field of image processing. The method comprises the steps of binarizing an infrared grayscale image to be processed by a preset way to obtain a binarized image; retaining the largest white communication region in the binarized image to obtain a first image containing the largest white communication region; retaining a plurality of black communication regions conforming to a preset condition in the first image to obtain a second image containing the plurality of black communication regions; and superimposing the infrared grayscale image and the second image to obtain a target grayscale image in which a target image is located in a region corresponding to the largest white communication region and background images located in regions corresponding to the black communication regions. The technical problem of interference of the background image in the infrared medical image with the target image is solved, and the technical effect of only retaining the target image while the background images in the infrared grayscale image are accurately, clearly and quickly eliminated, is achieved.

The invention relates to a method for culturing a high-quality efficient stress-resistant complete set line of large white pigs, which comprises the following steps of: building a basic group based on gene identification and disease resistance verification of F18 colibacillosis resistant major genes FUT1M307 of a core group, and culturing the high-quality efficient stress-resistant complete set line of the large white pigs by combining immunology principle, molecular breeding technology and conventional breeding means and using a candidate gene strategy. The complete set line pigs have the advantages of stress resistance, strong environmental adaptability, weaned piglet diarrhea and edema disease resistance, high lean meat rate, excellent meat quality, high feed reward and high birth number.

The accurately automatic human eye and face positioning process in digital grey scale image includes the steps of: setting distance between two eyes; performing 8 neighborhood comparison; changing too large white pixel block into black; changing the white pixel with too low neighborhood white pixel rate; changing the black pixel with too low neighborhood black pixel rate; change too large, too small or too perpendicular white pixel block into black one; repeated pairing white pixel blocks until two white pixel blocks meets the requirement as eye window; establishing two rectangular eye windows around two mass centers separately; calculating the mixed projection function of each windows; determining the position of eyes precisely with the mixed projection function; and scaling the originalimage and repeating the foregoing steps except the first one.

The invention discloses green, environment-friendly and dust-free chalk. A formula of the chalk is as follows: 15-20 parts of burnt gypsum powder, 15-20 parts of large white powder, 15-20 parts of water, and 3-5 parts of color paste, wherein the color paste is an aqueous solution comprising 2-5 parts of polyvinyl acetate emulsion, 2-3 parts of polyvinyl alcohol and 50 parts of water. The chalk has the characteristics that the chalk is fluent in writing, clear in handwriting, moderate in hardness and less in dust, and the dust does not rise easily.

The invention discloses a pig Oct4 gene insertion/lack detection method and application thereof. A boar genome DNA to be detected is used as a template; a primer pair P1 designed by referring to the pig genome is used as a primer; the Oct4 gene is amplified through a PCR (polymerase chain reaction) technology; then, agarose gel electrophoresis is performed. The insertion/lack polymorphism of the Oct4 gene in the NC_010449:g.2759-2760ins GGTTTTTGTCTA site is identified according to the electrophoresis result. The result shows that different types of the Oct4 gene 12-bp insertion/lack have the obvious relevance with the breeding properties of testicle weight, testicle long periphery length, testicle short periphery length and the like of 15-day-old large white boars; the result shows that different types of the Oct4 gene 12-bp insertion/lack can be used as DNA (deoxyribonucleic acid) marks of the boar breeding properties. The method is favorable for fast building the boar heredity resource groups with good breeding properties.

The invention relates to the field of animal breeding, in particular to a lean-meat-type pig selection method in a hybridization technique. The specific implementation steps comprise that first, local palm hair glutinous cereal pigs are selected as female parents which are crossed with boars which are landrace of an introduced variety and used as male parents, generated offspring boars are selected and are crossed with the offspring pigs generated by crossing of local glutinous cereal pigs used as male parents and large white sows of an introduced variety as female parents, excellent sows are selected as final female parents to be crossed with Duroc, and generated offspring pigs are high-quality lean-meat-type pork pigs. The novel-variety lean-meat-type pork pigs effectively overcome the shortcomings that outer-ternary piglets are high in diarrhea rate, high in meat moisture, low in unsaturated fattyacid, bad in meat flavor and the like.