Breeding method of new disease-resistance large white pig strain
A breeding method and technology of Large White pigs, applied in the field of breeding new disease-resistant strains of Large White pigs, can solve the problems of disease resistance and fecundity decline, conception rate of sows, and the survival rate of weaned piglets. Reduce the incidence rate, increase the annual genetic progress, and the effect of a long cycle
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[0051] (1) Carry out F18 Escherichia coli (purchased by the China Veterinary Drug Administration) infection test to the large white pig core group by adding feed, according to the results of phenotypic identification, set up the large white pig F18 Escherichia coli disease-resistant breeding base group;
[0052] 1) Bacterial culture Inoculate a single colony of F18 Escherichia coli into 3-5 mL of LB medium, culture it with shaking at 37°C overnight (about 18 hours), wash the bacteria with PBS repeatedly for 3 times the next day, and inoculate the Escherichia coli strain Dilute with PBS at a ratio of 1:20, and adjust the bacterial concentration to 4.6×10 8 CFU / L.
[0053] 2) Feed preparation According to the nutritional requirements of weaned piglets, feed pellets containing 21.7% crude protein. The feed does not contain any antibiotics and probiotics. The main purpose of preparing feed that does not contain antibiotics and probiotics is to avoid antibiotics in the feed. Inter...
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