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Breeding method of new disease-resistance large white pig strain

A breeding method and technology of Large White pigs, applied in the field of breeding new disease-resistant strains of Large White pigs, can solve the problems of disease resistance and fecundity decline, conception rate of sows, and the survival rate of weaned piglets. Reduce the incidence rate, increase the annual genetic progress, and the effect of a long cycle

Inactive Publication Date: 2017-07-25
TAICANG JINZHU AGRI DEV
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Problems solved by technology

Large white pigs play an extremely important role in the production of "three-way" hybrid commercial pigs in my country. The excellent characteristics of imported large white pigs are mainly high growth performance, but there are also problems and deficiencies such as disease resistance and reduced fecundity, especially in recent years. The incidence of diseases such as diarrheal disease in incoming piglets is high, which directly and indirectly leads to a decrease in the conception rate of sows, the number of piglets born alive and the survival rate of weaned piglets

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  • Breeding method of new disease-resistance large white pig strain
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  • Breeding method of new disease-resistance large white pig strain

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Embodiment

[0051] (1) Carry out F18 Escherichia coli (purchased by the China Veterinary Drug Administration) infection test to the large white pig core group by adding feed, according to the results of phenotypic identification, set up the large white pig F18 Escherichia coli disease-resistant breeding base group;

[0052] 1) Bacterial culture Inoculate a single colony of F18 Escherichia coli into 3-5 mL of LB medium, culture it with shaking at 37°C overnight (about 18 hours), wash the bacteria with PBS repeatedly for 3 times the next day, and inoculate the Escherichia coli strain Dilute with PBS at a ratio of 1:20, and adjust the bacterial concentration to 4.6×10 8 CFU / L.

[0053] 2) Feed preparation According to the nutritional requirements of weaned piglets, feed pellets containing 21.7% crude protein. The feed does not contain any antibiotics and probiotics. The main purpose of preparing feed that does not contain antibiotics and probiotics is to avoid antibiotics in the feed. Inter...

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Abstract

The invention discloses a breeding method of a new disease-resistance large white pig strain. The method includes the following steps that 1, a feed adding method is adopted to conduct an Escherichia coli infection experiment on weaned piglets of a core group in large white pigs, and an Escherichia coli resistant breeding basic group in the large white pigs is established; 2, concentration determination is conducted on cytokines of interleukin 1beta, interleukin 4, interleukin 6, interleukin 8, interleukin 10, transforming growth factor beta, tumor necrosis factor alpha and interferon gamma of an Escherichia coli resistant population in the basic group; reproductive performances are detected and selected, growth speeds, lean meat ratios and carcass quality characters, and a first generation is established; 3, a group subculture selective breeding method and a molecular-marker-assisted selection method are adopted. In this way, by the adoption of the breeding method of the new disease-resistance large white pig strain, after four to five generations, a vested breeding target is achieved gradually, a new-strain core group is established, and the morbidity of piglet diarrheal diseases is expected to be reduced.

Description

technical field [0001] The invention relates to the fields of animal genetics, breeding, reproduction and molecular biology, in particular to a method for cultivating a new disease-resistant strain of Large White pigs. Background technique [0002] my country's pig industry has a strong pig production system, which is often said that "my country is a big country for pig farming", but the top core breeding pig resources in the "pyramid" breeding system have long relied on imports. For a long period of time, my country has been in a vicious circle of "introduction → maintenance → degradation → re-introduction" for breeding pigs. After joining the WTO, this situation has become more serious. In short, the obvious dependence on the introduction of breeding pigs is very unfavorable to the sustainable development of my country's pig industry. Considering the long-term interests of the country, this phenomenon should not continue to develop. Faced with this situation, we must chan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A23K10/18A23K50/30A23K50/60G01N33/53
CPCA01K67/02G01N33/53
Inventor 王海斌蔡家嘉包文斌叶兰喻礼怀魏宗友高清清
Owner TAICANG JINZHU AGRI DEV
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