98 results about "Large white pig" patented technology

The invention relates to the technical field of molecular markers, and discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the litter size character of pigs and application of the SNP molecular marker. The SNP molecular marker is located at a 34977540bp site of a chromosome 2 of an international pig reference genome Sscrofa11.1 version, and the nucleotide sequence of the site is G or A and is located in an exon region of an LUZP2 gene. The invention also discloses an application of the SNP molecular marker in breeding of the litter size character of the boar, and provides a method for breeding or auxiliary breeding of the boar with high / low litter size based on the marker. The invention also provides a specific primer for identifying the SNP site. By applying the technologies, accurate prediction and efficient screening of the litter size of the large white pigs can be achieved, the breeding efficiency of excellent breeding pigs is remarkably improved, and the population improvement process is accelerated. The invention also provides important technical support for sustainable development of pig raising industry, also provides theoretical basis for research in related fields, and is expected to promote further development of pig genetic improvement work.

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP molecular marker for the birth litter weight character of a large white pig and application of the SNP molecular marker, an SNP site is located at the 31744436th site of a 10th chromosome of a pig genome Sus scrofa 11.1 version, and nucleotide at the SNP site is A / G; the SNP site is located at the 31744633th site of the 10th chromosome of a pig genome Sus scrofa 11.1 edition, and the nucleotide at the SNP site is A / G. The two SNP molecular markers can serve as effective molecular markers to be applied to large white pig newborn litter weight character molecular marker-assisted selection breeding, high accuracy is achieved for rapidly and accurately screening large white pigs with newborn litter weights, and the breeding efficiency of the large white pigs can be remarkably improved; the method has potential application value in large-scale molecular precise breeding of large white pigs.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker influencing the backfat thickness character of a large white pig and application of the SNP molecular marker, and belongs to the technical field of pig character genetic improvement, and the SNP molecular marker is located at a basic group of a 66584554 site of a fifth chromosome of a pig genome Sus scrofa 11.1 version. The SNP site related to the backfat thickness character of the large white pig, provided by the invention, is detected and can be used for breeding pig early-stage selection and remaining, that is, specific genotype individuals under the site are selected for breeding remaining in actual breeding work, so that the accuracy and breeding efficiency of large white pig meat character selection are improved, and the breeding efficiency is improved. Meanwhile, the gene frequency related to excellent characters in the population can be improved, and the backfat thickness character of the population is improved in a targeted manner, so that the production performance and the economic benefit of the whole population are improved.

The invention belongs to the technical field of molecular biology, and relates to a large white pig backfat thickness character related SNP (Single Nucleotide Polymorphism) marker as well as a detection method and application thereof. The SNP marker is located on a nucleotide sequence of a melanocortin-4 receptor gene (MC4R) on a pig chromosome 1, the site of the SNP marker is a g.160773437 nucleotide site on the pig chromosome 1 of a reference sequence of an international pig genome version 11.1, the SNP marker has G / A polymorphism, and the SNP marker is significantly related to the backfat thickness character of the large white pig. The SNP marker provided by the invention is related to the pig backfat thickness character, a pig strain with low backfat thickness can be screened by identifying the SNP marker, and the obtained pig strain with high lean meat percentage has important economic benefits and social values.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP marker influencing the content of porcine inosine and application of the SNP marker. The 10 SNP molecular markers closely related to the porcine inosine content are researched and determined on the basis of American-series purebred landrace, American-series purebred large white pigs and American-series purebred duroc, the dominant allele frequency can be increased generation by generation by optimizing the dominant allele of the SNP, the inosine content can be reduced, and the molecular markers can be used for identifying the porcine inosine content. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP marker influencing pig UMP content and application thereof. Whole genome re-sequencing and GWAS analysis are adopted, American-series purebred landrace pigs, American-series purebred large white pigs and American-series purebred duroc are used as research objects, seven SNP markers related to the pig UMP content are researched and determined, by optimizing dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the UMP content can be increased, and the pig UMP content can be improved. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention belongs to the technical field of molecular biology, and discloses a large white pig residual feed intake character related PPIL4 gene molecular marker as well as a detection method and application thereof. The SNP marker is located on a nucleotide sequence of a peptidyl proline isomerase-like protein-4 gene (PPIL4) on a pig chromosome 1, the site is a g.16442886 nucleotide site on the pig chromosome 1 of a reference sequence of an international pig genome version 11.1, the SNP marker has C / T polymorphism, and the SNP marker is significantly related to the residual feed intake character of the large white pig. The SNP marker provided by the invention is related to the residual feed intake character of the pig, the pig strain with low residual feed intake can be screened by identifying the SNP marker, and the obtained pig strain with low feed conversion ratio and high growth speed has important economic benefits and social values.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker associated with a pig backfat thickness character and application. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO: 1; one SNP molecular marker site A / G is arranged at the 254bp position of the sequence of the SNP molecular marker. Genetic typing is carried out by utilizing a gene chip technology, whole genome association analysis is carried out in combination with the character of correcting the 100 kg backfat thickness of a large white pig, one SNP molecular marker remarkably related to the character is screened out, and a new marker resource is provided for molecular breeding of the growth character of the breeding pig.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the feed conversion rate of large white pigs and application thereof, and belongs to the technical field of molecular marker-assisted selection. According to the SNP molecular marker related to the feed conversion rate of the large white pigs disclosed by the invention, DNA samples of 5256 large white pigs are subjected to genetic typing by utilizing a Porcine 80K SNP high-density chip, and an SNP site remarkably related to the feed conversion rate of the pigs is finally screened out through genome-wide association analysis (GWAS). A site genome version of the SNP molecular marker corresponds to a 263045504 site on a No.1 chromosome of an Enmbl Sscrofa 11.1 reference genome, a polymorphic site of the SNP molecular marker is G or A, and when the genotype of the site is G / G, the pig has a relatively high feed utilization rate.

The invention discloses the technical field of pig eye muscle areas, and particularly relates to a molecular marker associated with the pig eye muscle area. The molecular marker comprises a nucleotide sequence formed by A / G single nucleotide polymorphism at the 3593429nt position of chromosome 10 of the international pig reference genome 11.1 version of pigs. The SNP marker provided by the invention is remarkably related to the eye muscle area of the large white pig muscle, a large white pig strain with the large eye muscle area can be screened by identifying the SNP marker, and the obtained large white pig strain with the large eye muscle area has important economic benefits, edible value and social value.

The invention is suitable for the technical field of molecular genetics, and provides an SNP molecular marker related to pig backfat thickness and application thereof. The invention studies and determines that the pig backfat thickness related molecular marker is located at the 95583333rd site (corresponding to the 701st basic group A / G mutation of SEQ ID NO: 1) of the No.18 chromosome of the international pig reference genome version 11.1, and verifies the influence effect of the molecular marker on the pig backfat thickness character. And when the basic group at the site is A, the pig has lower back fat thickness. On the basis, a molecular marker-assisted selection breeding technology for rapidly improving the backfat thickness character of the pig is established, the frequency can be increased generation by generation by optimizing the dominant allele (A), the backfat thickness of the breeding pig is remarkably reduced, the genetic improvement process of commercial pig herds containing landrace and large white pig blood lineage is accelerated, and the breeding efficiency is improved; the sales profit is increased for enterprises, the core competitiveness is improved, and the economic benefits of commercial pigs are improved.

The invention belongs to the technical field of animal genetic breeding, and provides an application of an SNP molecular marker related to a pig backfat thickness character. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 7, wherein the polymorphism of the eleventh basic group is C / T. The key gene SLC26A3 related to the backfat thickness of the large white pig is identified through whole genome deep sequencing, the genotype of the backfat thickness character of the large white pig is further detected through an SLC26A3 gene molecular marker, and the backfat thickness of the CC genotype large white pig is obviously higher than that of a CT / TT genotype pig. Therefore, the breeding of the CT / TT genotype individuals is beneficial to meeting the continuous demand of large-scale production on high-efficiency utilization of feed energy. The detection method provided by the invention is simple and easy to operate, can efficiently and quickly identify the back fat thickness character of the large white pig, improves the uniformity of the back fat thickness of the pig, and provides a scientific basis for early breeding of the pig.

The invention discloses an rs338618851 site SNP (Single Nucleotide Polymorphism) molecular marker related to the meat production efficiency of pigs and application of the rs338618851 site SNP molecular marker. The genetic marker is cloned from a 93991071th site of a pig chromosome 6. The method comprises the following steps: selecting American-series large white pigs as test materials, extracting whole genome DNA from pig blood, and designing primers according to a pig genome sequence NC010448.4 published by an NCBI (National Center of Biotechnology Information) database; a base substitution of T or C exists at the 172nd site of the primer amplification sequence, and the substitution causes the generation of polymorphism at the position. According to the present invention, the SNP site is subjected to typing through the direct sequencing technology, and the correlation analysis results show that compared with other gene individuals, the 172nd TT genotype individual has characteristics of major birth, large eye muscle area, short time of 100 kg day age, and the like; the invention provides a new marker for molecular breeding of pig meat traits.

The invention belongs to the technical field of molecular biotechnology and molecular markers, and discloses a pig nipple number character related SNP molecular marker and application thereof, the site of the SNP molecular marker corresponds to the nucleic acid site at the 103001352bp site of the No.7 chromosome of the international pig reference genome Sscrofa11.1 version sequence, and the site is Ggt; and A basic group mutation. The SNP molecular marker associated with the nipple number character of the large white pig can be applied to genetic improvement of the growth and reproduction character of the large white pig, provides a new genetic marker resource for molecular marker-assisted selection of the nipple number character of the large white pig, can be applied to breeding of sows with high lactation capacity and high piglet survival rate, accelerates the breeding process, and improves the breeding efficiency. The reproductive performance of offspring large white pigs is improved.

The invention belongs to the technical field of molecular biology, particularly relates to a pig chromosome 3 TMC7 gene as a meat-producing character molecular breeding marker and application, and aims to provide a new marker and technical support for meat-producing character molecular breeding of pigs. The molecular marker is located at the 26346502 base in a pig chromosome 3 TMC7 gene, and allele G / T polymorphism exists at the site. A correlation analysis result shows that in American and French large white pig populations, the eye muscle area of a GG genotype individual is remarkably higher than that of other genotype individuals (plt; 0.05), and it is indicated that the genotype is a favorable genotype of the eye muscle area character. The SNP marker provided by the invention provides a new molecular tool for genetic breeding of pig eye muscle area characters, and can be used for auxiliary screening of breeding pigs with excellent meat production performance, so that the breeding process of pig breeds with high lean meat percentage is accelerated.

The invention discloses a CHRM3 gene InDel molecular marker associated with growth and reproduction traits of a large white pig and application of the CHRM3 gene InDel molecular marker, a 62bp deletion variation is identified in a second intron of the CHRM3 gene, and a breakpoint is positioned in a second intron 521426525142713bp (a pig chromosome 14 reference sequence NC010456.5) of ENSSSCT00000082112.1. The application for detecting / identifying the growth and reproduction traits of the large white pigs is developed on the basis of the molecular marker, and the detection method is simple, rapid and low in cost and can be used for accurately screening individuals with better traits such as backfat thickness, primary birth litter weight, total multiparous litter size and gestation period of multiparous sows in the early stage, so that genetic improvement is carried out on the growth and reproduction traits of the pigs, and the economic benefit is increased. Multiple characters such as growth and reproduction can be selected at the same time, the breeding period of excellent boars can be shortened, and the breeding efficiency and progress are accelerated.

The invention belongs to the technical field of pig molecular markers, and particularly discloses an SNP molecular marker related to the character of the number of healthy piglets born by a sow and application of the SNP molecular marker. Genetic typing and whole genome association analysis are carried out on 732 large white sows by using second-generation gene sequencing and gene chip technologies, an SNP site significantly related to the character of the number of healthy piglets born by the large white sows is screened out, the SNP site is located at the upstream of an SLC22A12 gene of a pig chromosome 2 and corresponds to the 7565970th nucleotide of a chromosome 2 of an international pig 11.1 version reference genome, and the SNP site is located at the upstream of the SLC22A12 gene of the chromosome 2 of the pig 11.1 version reference genome and is located at the upstream of the SLC22A12 gene of the chromosome 2 of the pig 11.1 version reference genome. The method can be used for character prediction of healthy litter size of sows or auxiliary breeding.

The application belongs to the technical field of pig molecular marker, and particularly discloses a SNP molecular marker related to the litter size of sows and application thereof. SLC22A12 A SNP site significantly related to the litter size of Landrace sows is screened out by using second-generation gene sequencing and gene chip technology for genotyping and whole genome association analysis of 732 Landrace sows, the SNP site is located on the upstream of a gene on chromosome 2 of pigs, corresponding to the 7565970th nucleotide on chromosome 2 of the international pig 11.1 version reference genome, and can be used for predicting or assisting breeding of the litter size of sows.

The invention relates to the technical field of pig breeding improvement, in particular to a method for evaluating backfat of a large white pig based on a USP16 gene, which is used for determining an SNP (Single Nucleotide Polymorphism) molecular marker of the large white pig and judging whether the SNP molecular marker is one of the following SNP molecular markers: g.192401821Ggt located in an intron region of the USP16 gene; site A; the g.192407026Ggt is positioned in a downstream regulation and control region of the USP16 gene; site A; the g.192407237Agt is positioned in a downstream regulation and control region of the USP16 gene; a G site; the g.192408287Tgt is positioned in a downstream regulation and control region of the USP16 gene; a C site; the g.192408499Cgt is positioned in a downstream regulation and control region of the USP16 gene; and a T site. The evaluation method provided by the scheme and the used SNP molecular marker provide good theoretical support and effective guidance for breeding and improvement of growth traits of pigs.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the back fat thickness character of a large white pig and application of the SNP molecular marker. The invention belongs to the technical field of pig molecular markers, and particularly discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a pig backfat thickness character. DNA (deoxyribonucleic acid) samples of 5253 large white pigs are collected, a pig 80K SNP (single nucleotide polymorphism) high-density functional site chip is used for genetic typing, a molecular marker related to the back fat thickness character of the pigs is obtained by means of whole genome association analysis and screening, the registration number of the marker in an Ensepbl database is rs332090971, a polymorphic site is located at the 7932236th site of a chromosome 11, an A / C allele mutation exists, and the molecular marker is a molecular marker related to the back fat thickness character of the pigs. When the polymorphic site is C, the pig has a thinner back fat thickness character.

The invention provides an SNP (Single Nucleotide Polymorphism) marker primer pair related to pig multirib traits and application of the SNP marker primer pair. The SNP site is a nucleotide site of chromosome 7 rs701714758 of a reference sequence of an international pig genome version 11.1, has G / A polymorphism, is located in a protein coding region of an ABCD4 gene, and is remarkably associated with rib number characters of Duroc pigs, landrace pigs, large white pigs and Pietrain pigs through verification of whole genome association analysis and meta analysis. The invention further provides a primer pair for detecting the SNP marker, an upstream primer of the primer pair is SEQ ID NO: 2, and a downstream primer of the primer pair is SEQ ID NO: 3. The SNP marker can be applied to marker-assisted selection and whole genome selection of rib number characters of multiple varieties of pigs, can accurately screen a multi-rib number pig group, improves character prediction accuracy, increases pork and rib row yield, meets consumption demands, and has both social and economic benefits.

The present invention discloses a SNP molecular marker related to the feed conversion rate of Large White pigs and its application, belonging to the technical field of molecular marker-assisted selection. The present invention discloses a SNP molecular marker related to the feed conversion rate of Large White pigs. The DNA samples of 5256 Large White pigs were genotyped using the Porcine 80K SNP high-density chip, and a SNP site significantly correlated with the feed conversion rate of pigs was finally screened out through genome-wide association analysis (GWAS). The site genomic version of the SNP molecular marker corresponds to position 263045504 on chromosome 1 of the Ensembl Sscrofa 11.1 reference genome. The polymorphic site is G or A. When the genotype of the site is G / G, the pig has a higher feed utilization rate.

The present invention belongs to the field of molecular biology technology and relates to a SNP marker of the FRMD4A gene associated with the teat number trait of Large White pigs, a detection method thereof, and an application thereof. The SNP marker is located on the nucleotide sequence of the FRMD4A gene on pig chromosome 10. The site of the SNP marker is the g.48019215 nucleotide site on pig chromosome 10 in the International Porcine Genome Version 11.1 reference sequence, and has a C / T polymorphism. The SNP marker is significantly associated with the teat number trait. The SNP marker provided by the present invention is associated with the teat number trait of pigs. By identifying the SNP marker, pig strains with a high teat number can be screened. The resulting high-fertility strains with a high teat number have important economic benefits and social value.

The invention belongs to the technical field of animal molecular breeding, and particularly relates to application of a haplotype molecular marker for improving the utilization efficiency of large white pig feed, and the haplotype molecular marker comprises a haplotype 1 and / or a haplotype 2; the haplotype 1 comprises 8 sites of SNP2-SNP9 in total, and the haplotype 2 comprises 26 sites of SNP10-SNP35 in total; the application refers to any one of the following steps: identifying the feed conversion rate of the large white pigs; the feed utilization rate of large white pig offspring is improved. The haplotype H002 individuals and the haplotype H004 individuals are individuals with low feed conversion rate in each LDblock haplotype module, and the haplotype H002 individuals and the haplotype H004 individuals are individuals with low feed conversion rate in each LDblock haplotype module. In the actual breeding process, H002 and H004 haplotype individuals are reserved, screening of individuals with high feed utilization efficiency and seed reserving are facilitated, the accuracy of early-stage seed selection of the feed utilization efficiency character of the large white pigs is improved, and good economic benefits and wide application prospects are achieved.

The application relates to a product of a SNP molecular marker related to the litter weight trait of Large White pigs, wherein the SNP molecular marker is an A>G base mutation at a nucleic acid site of 116767089 of chromosome 2 in the international pig genome Sscrofa 11.1 version, and the nucleotide sequence is shown as SEQ ID No. 1; the product at least comprises an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is shown as SEQ ID No. 2, and the nucleotide sequence of the downstream primer is shown as SEQ ID No. 3. The SNP molecular marker can be used for breeding, cultivation and evaluation of multi-species high-reproduction-performance sows.

The application belongs to the technical field of animal molecular breeding, and particularly relates to application of a SNP haplotype molecular marker related to average daily weight gain of Large White pigs, wherein the SNP haplotype molecular marker comprises 12 loci of SNP1-SNP12, when the combination of SNP1-SNP12 is TTGCCCCACAGT in turn, the average daily weight gain of the Large White pig is higher than that of the combination of TCATATTTTGGC; the application refers to any one of the following: identifying the average daily weight gain of the Large White pig; improving the average daily weight gain of offspring of the Large White pig. Based on the application, in the actual breeding process, the H001 (TTGCCCCACAGT) haplotype individual is reserved, which is beneficial to screening of the high average daily weight gain individual and breeding, and improves the accuracy of early selection of the average daily weight gain trait of the Large White pig, and has good economic benefits and wide application prospect.

The invention belongs to the technical field of molecular biology, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker of an HTR2A gene related to a pig backfat thickness character and application of the SNP molecular marker. The molecular marker is from the 20563279 basic group in a pig 11 # chromosome HTR2A gene. A large white pig is selected as a test material, whole genome DNA is extracted from pig blood, and primers are designed according to a pig genome sequence (NC010449.5) published by an NCBI database. And a C or T base substitution exists at the 316th site, so that polymorphism is caused. According to the present invention, the SNP site is subjected to typing, and the association analysis on the American line big white and the French line big white shows that the back fat thickness of the CC genotype individual is significantly thin compared with the back fat thickness of the other genotype individuals; the invention provides a new marker for pig molecular marker-assisted selection, and the new marker is applied to genetic improvement of pig backfat thickness to accelerate the breeding improvement process of high-quality pigs.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP (Single Nucleotide Polymorphism) marker capable of influencing the content of IMP or GMP (Good Manufacturing Practice) of pigs and application of the SNP marker. According to the invention, based on three test swinery of American-series purebred landrace, American-series purebred large white pig and American-series purebred Duroc, pig whole genome re-sequencing and GWAS analysis are adopted, 15 SNP markers related to the content of IMP or GMP are researched and determined, and by optimizing the dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the content of IMP or GMP can be increased, and the number of SNP markers related to the content of IMP or GMP can be increased. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

According to the application of the product of the SNP molecular marker related to the birth litter weight character of the large white pig, the SNP molecular marker is as follows: A > G base mutation located at the 116767089 nucleic acid site of the second chromosome of the international pig genome Sscrofa 11.1 version, and the nucleotide sequence of the SNP molecular marker is as shown in SEQ ID No.1; the product at least comprises an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is as shown in SEQ ID No.2, and the nucleotide sequence of the downstream primer is as shown in SEQ ID No.3. The SNP molecular marker provided by the invention can be used for breeding, breeding and evaluating multi-variety sows with high reproductive performance.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to pig fast growth character and application thereof, and relates to the technical field of molecular markers. The SNP molecular marker is located at a 55754471bp site of a No.17 chromosome of an international pig reference genome Sscrofa 10.2 version, and the nucleotide sequence of the site is C or T and is marked as Cgt; t is located in the SULF2 gene, and T is an excellent allele related to high growth speed. The SNP site provided by the invention is used for breeding pig breeds with high growth speed, and pigs with excellent growth performance can be effectively and accurately screened through combined use of primers and kits designed for the SNP molecular marker and a method for carrying out rapid-growth pig breeding or auxiliary breeding based on the marker. According to the method, accurate prediction and efficient screening of fast-growing individuals of large white pigs can be achieved, the breeding efficiency of excellent breeding pigs is remarkably improved, and the population improvement process is accelerated.