93 results about "Large white pig" patented technology

The invention discloses a structural variation 177 (SV177) for distinguishing varieties of large white pigs and Chinese indigenous pigs. The SV177 is characterized in that the SV177 is positioned at chrX: 100561156-100562159 of a pig reference genome Sscrofa 10.2, missing genes of the SV177 are determined as D, and normal genes which are not missed are determined as A. When a detection technology provided by the invention is used for detecting the SV177 genotype of a sample, the large white pigs have three genotypes and give priority to the genes A which are not missed, and the Chinese indigenous pigs are free from a heterozygous genotype (DA) and give priority to the missing genes D; therefore, the SV177 can be used for distinguishing the varieties of the large white pigs and the Chinese indigenous pigs as a molecular marker.

The invention belongs to the technical field of porcine molecular marker screening, and in particular relates to porcine NTF3 promoter region SNP as a molecular marker for the boar breeding traits and applications of the porcine NTF3 promoter region SNP. The molecular marker is cloned from the 5' flank promoter fragment of the porcine NTF3 gene, the nucleotide sequences of the molecular marker are shown as SEQ ID NO:1 and SEQ NO:2, allele mutation of A/G and G/A exists at the 92nd basic group site of the sequence shown as SEQ ID NO:1 and SEQ NO:2, and the allele mutation causes the polymorphism of MvaI-RFLP. The marker is utilized for carrying out correlation analysis on the boar breeding traits of large white pigs and duroc pigs. The breeding traits comprise the boar sperm motility and the sperm density trait. The invention further discloses a typing detection method of the marker, and therefore, a novel marker is provided for selecting the boar breeding traits. The marker can be used for evaluating the boar breeding traits and researching the genetic improvement on the breeding boar breeding performance.

The invention discloses a molecular marker SV193 for distinguishing Chinese local pig breeds from Large White pigs and an application technology of the molecular marker SV193. The molecular marker SV193 is characterized in that SV193 is located at chr8:142093174-142093446 of a pig reference genome Sscrofa 10.2, the deleted gene of SV193 is set to be D, and the normal undeleted gene is set to be A. With the application of the detection technology, the results that the Large White pigs mainly rely on the undeleted gene A while the Chinese local pig breeds mainly rely on the deleted gene D are obtained, and the Large White pigs can be distinguished from the Chinese local pig breeds by the application of the molecular marker SV193.

The invention discloses a structural variation SV200 molecular marker for identifying local pig breeds. The structural variation SV200 molecular marker is characterized in that the structural variation SV200 is positioned at chr 6: 17953135-17953289 of a pig reference genome Sscrofa 10.2; a deletion fragment has the length of 155bp and is positioned in an intron 5 of an MMP-15 gene; the deletion gene of the structural variation SV200 is defined as D, a normal non-deletion gene is defined as A, and the frequency of a D gene in 5 local pig breeds is significantly higher than a corresponding value of a big white pig. The structural variation SV200 molecular marker can be used as the molecular marker for identifying the local pig breeds.

The invention discloses a method for identifying pig backfat thickness based on rs80995809 locus genotyping and its application. The method is used to detect whether the genotype of the rs80995809 locus in a pig individual is a GG genotype, a TT genotype or a GT genotype, according to the genotype of individual pigs, the backfat thickness of pigs is determined: the backfat thickness of the pigs with GG genotype is thicker than the backfat thickness of the pigs with GT genotype, and the backfat thickness of the pigs with GT genotype is greater than the backfat thickness of the pigs with TT genotype. The experiment proves that the rs80995809 locus has a significant effect on the pig backfat thickness, the selected SNP can be used as a molecular genetic marker, the numbers of trait loci thatare related or closely linked to the backfat thickness can be looked for, which can directly perform genotype selection or marker-assisted selection for large white pigs, and the breeding of dominantvarieties of the large white pigs can be accelerated.

The invention discloses a breeding method for producing high-quality synthetic-line commercial pigs by utilizing Luchuan pigs. The breeding method includes that male parents selected from large white pigs hybridize with female parents which are also selected from the large white pigs, and high-quality large white male pigs are selected from the produced hybrid generations to serve as final male parent breeds; the local Luchuan pigs are selected as female parents of the synthetic-line pig parent generation and hybridize with landrace serving as the male parents of the synthetic-line pig parent generation, and high-quality female Changlu pigs are selected from the produced hybrid generations to serve as female parent breeds; the selected male parents and the selected female parents are subjected to hybridization, and the hybrid generations serve as the high-quality synthetic-line commercial pigs which are then put into production. The breeding method for producing the high-quality commercial-line commercial pigs by utilizing the Luchuan pigs has the advantages that the high-quality local pig breeds, namely the Luchuan pigs, of Guangxi are utilized for breeding a special strain of the Luchuan pigs, and the large white pigs, the landrace and the Luchuan pigs are subjected to three way hybridization to form a complete set for producing high-quality commercial pork pigs, wherein the Luchuan pigs serve as the female parents in the synthetic-line pig parent generation, the landrace serves as the male parents in the synthetic-line pig parent generation, the Changlu female parent pigs are obtained by means of hybridization, and the large white pigs serving as the synthetic-line final male parents hybridize with the Chuanglu female parent pigs so as to produce high-quality Landrace Luchuan commercial pigs.

The invention discloses an SNP molecular marker relevant to growth traits of a large white pig and application of the SNP molecular marker. The invention belongs to the molecular marker breeding technical field. The SNP molecular marker is located on the locus rs701332503 of the gene DHRS4 of the large white pig. The nucleotide sequence of the gene DHRS4 in the large white pig is relevant to the growth traits of the large white pig from the 5' end locus rs701332503, that is to say, the SNP molecular marker on the locus rs701332503 of the gene DHRS4 of the large white pig is relevant to the growth traits like the feed conversion ratio of the large white pig and is a new molecular marker, by determining the genotype of the SNP locus of the to-be-tested large white pig, the growth traits likethe feed conversion ratio of the large white pig are subjected to breeding pig sifting, the production cost can be effectively reduced, genetic progress can be accelerated, sifting of large white breeding pigs is better served, the huge market requirement can be met, and the SNP molecular marker also has high economic application value and scientific research value.

The invention relates to a method for culturing a high-quality efficient stress-resistant complete set line of large white pigs, which comprises the following steps of: building a basic group based on gene identification and disease resistance verification of F18 colibacillosis resistant major genes FUT1M307 of a core group, and culturing the high-quality efficient stress-resistant complete set line of the large white pigs by combining immunology principle, molecular breeding technology and conventional breeding means and using a candidate gene strategy. The complete set line pigs have the advantages of stress resistance, strong environmental adaptability, weaned piglet diarrhea and edema disease resistance, high lean meat rate, excellent meat quality, high feed reward and high birth number.

The invention belongs to the technical field of pig molecular marker screening, and particularly relates to a SNP (single nucleotide polymorphism) molecular marker related to the primary mating day age of a sow. The accession number of the SNP molecular marker is rs81253440, a Danish landrace sow ear sample from a certain piggery in the South China is collected, genome DNA is extracted, quality detection is carried out, and a GeneSeek Porcine50K SNP superchip is used for carrying out genetic typing to obtain SNP typing data so as to obtain the SNP molecular marker related to the primary matingday-age characters of the sow. The nucleotide sequence of the SNP molecular marker is disclosed in SEQ ID NO:1, the allele mutation of a G/T is in the presence in the 51st basic group of the sequence, and the mutation causes the nucleotide of the disclosed sequence to generate polymorphism; and when the 51st nucleotide on the nucleotide sequence is T, the primary mating day age of the sow is judged to be increased.

The present invention relates to the husbandry technology field, and especially relates to a method for hybridizing a high-quality boar commercial strain with a commercial pig. The method comprises the steps of (A) using a fluothane-free recessive gene berkshire as a first male parent of the commercial strain, using a duroc as a first female parent of the commercial strain, and hybridizing and obtaining a badu hybrid pig; (B) using a landrace as a second male parent of the commercial strain, using a large white pig as a second female parent of the commercial strain, and hybridizing and obtaining a grown binary pig; and (C) selecting the badu hybrid pig obtained by hybridizing in the step (A) as a terminal male parent of the commercial strain, using the grown binary pig obtained by hybridizing in the step (B) as a terminal female parent of the commercial strain, and hybridizing and obtaining a high-quality commercial pig. The high-quality commercial pig grows fast with a speed of 726g/d, has uniform fat and lean meat without PSE meat, and can mature in advance for about 10-15 days, meat quality trait is good, product quality is reliable, market prospect is good, and production efficiency can be improved.

The invention relates to the field of livestock breeding, in particular to a breeding method for five-line crossbreeding mating lean type boars. The method includes the following steps that S1, Pietrain pigs are selected as paternal line male parents, Duroc pigs are selected as maternal line female parents, and Pietrain*Duroc pigs are obtained through crossbreeding to be used as terminal male parents; S2, Landrace pigs are selected as maternal line male parents, large white pigs are selected as maternal line female parents, and Landrace*large white pigs are obtained through crossbreeding to beused as maternal line progenitor female parents; S3, another Landrace pigs are selected as progenitor male parents to be crossbred with the Landrace*large white pigs obtained the second step, so thatLandrace*(Landrace*large white) pigs are obtained and used as terminal female parents; S4, the Landrace*large white pigs are crossbred with the Landrace*(Landrace*large white) pigs are crossbred, sothat five-line crossbreeding mating lean type boars are obtained. The commercial pork pigs bred through the method are uniform in appearance and high and long in body; the heads of the pigs are in a medium size, and the mouths of the pigs have a medium length and are wide; the backs and waists are flat and straight, bellies are slightly curled, and four limbs are stout; the economic body weight islarge in the market, and meat quality is excellent; production efficiency is high.

The invention discloses an SNP site combination and a method for predicting alive litter size genetic performance of to-be-tested pigs. The SNP site combination is composed of the 501st nucleotide from the 5'-terminal in the sequence 1, the 501st nucleotide from the 5'-terminal in the sequence 2, the 501st nucleotide from the 5'-terminal in the sequence 3, the 501st nucleotide from the 5'-terminal in the sequence 4 and the 501st nucleotide from the 5'-terminal in the sequence 5 in the genome of pigs. A test proves that prediction reliability value is 0.081 by means of the five SNP sites screened in the invention, which is increased by 13.8% than a BLUP method, so that by means of the five SNP sites, the genetic value of alive litter size of to-be-tested large white pigs can be predicted. The SNP site combination can accelerate genetic progress, brings economic benefit to breeding farmers, and has important application value.

The invention belongs to the field of animal genetic engineering and is characterized in that: six different promoter deletion segments, which are MSTN-P1, MSTN-P2, MSTN-P3, PSTN-P4, MSTN-P5 and MSTN-P6 respectively, at the upstream of an MSTN gene are cloned from a large white pig; the segments are established in pGL3-Basic vectors to determine activities thereof; the MSTN-P2 has stronger activity and the nucleotide sequence thereof is shown as a sequence table SEQ ID NO:2. By adding SV40 enhancer segments into the MSTN-P2, the nucleotide sequence of the MSTN-P2 is shown as a sequence table SEQ ID NO:3. The promoter segments are found to be greatly enhanced in activities through transfected muscle-derived cells and non-muscle-derived cells. The invention discloses the establishment progress of the fusion promoters, thus providing a new genetic resource for genetically modified animals or induced foreign genes for establishing muscle specificity in high-efficiency expression in muscular tissues.

The invention relates to novel circRNA related to intramuscular fat and application thereof, in particular to circRNA_08840 related to intramuscular fat and new application of an interaction gene ssc-miR-339-3p of circRNA_08840. The two kinds of typical pigs which are large white pigs and Laiwu pigs are selected as research objects, a biological information science method is used for conducting sequencing and data analyzing on circRNA, miRNA and an interaction relation of circRNA and miRNA in the intramuscular fat of the two kinds of pigs, several quite good intramuscular fat content marks arefound, and experimental results show that circRNA_08840 and the interaction gene ssc-miR-339-3p of circRNA_08840 are remarkable in expression difference of intramuscular fat tissue of the two kinds of typical pigs, and novel circRNA is expected to breed pigs with different meat qualities as a mark in molecule auxiliary breeding.

The invention relates to novel application of lncRNA and a trans-regulation gene WNT11 thereof. The novel application is characterized in that adipose tissue gene expression profiles in large white pigs and Laiwu pigs are compared and analyzed by using a high-throughput sequencing technology, key differential expression lncRNA related with adipogenic differentiation and lipid metabolism is screened out, and expression of signaling pathway family member WNT11 of the lncRNA regulation gene Wnt is displayed by further analysis. According to the invention, comprehensive analysis on mRNA and lncRNAfor regulating procine intramuscular fat deposition is carried out, and certain reference for breeding porcine breeds with high meat quality as well as researching prevention and treatment of lipid metabolism-related diseases is provided.

The invention relates to the field of feeds, in particular to a large white pig feeding method. The method comprises a summer feeding method and a winter feeding method, wherein 1) the summer feeding method comprises the following steps: feeding the pigs with a feed which is composed of the following components in parts by weight: 35-40 parts of tuberous-rooted mustard, 25-30 parts of barley, 10-15 parts of oil cake, 8-10 parts of watermelon peel, 6-8 parts of silkworm excrement, and 3-5 parts of dandelions; 2) the winter feeding method comprises the following steps: feeding the pigs with a feed which is composed of the following components in parts by weight: 30-35 parts of corn, 15-20 parts of bran, 15-20 parts of barley, 10-15 parts of Shiitake mushroom, 5-8 parts of peanut leaves, 3-5 parts of earthworms and 2-5 parts of vinasse. The feed formulas in summer and winter are different, the temperature and the cleaning frequency of piggeries in summer and winter are controlled to optimize the feeding environment of the large white pigs, the quality and the nutritive value of the pork of the large white pigs are improved, the growth and development time is shortened; the feed has no additive and is environmentally friendly, the pollution is avoided and the breeding cost is low.

The invention discloses an SNP site combination and a method for predicting litter birth weight genetic performance of to-be-tested pigs. The SNP site combination is composed of the 501st nucleotide from the 5'-terminal in the sequence 1, the 501st nucleotide from the 5'-terminal in the sequence 2, the 501st nucleotide from the 5'-terminal in the sequence 3, the 501st nucleotide from the 5'-terminal in the sequence 4 and the 501st nucleotide from the 5'-terminal in the sequence 5 in the genome of pigs. A test proves that prediction reliability value is 0.033 by means of the five SNP sites screened in the invention, which is increased by 129.7% than a BLUP method, so that by means of the five SNP sites, the genetic value of litter birth weight of to-be-tested large white pigs can be predicted. The SNP site combination can accelerate genetic progress, brings economic benefit to breeding farmers, and has important application value.

The invention belongs to the field of preparation of molecular markers of pigs, and particularly relates to identification and application of SNP in fourth exon region of INCENP of pigs. An SNP molecular marker associated with the propagation property of boars is obtained, the nucleotide sequence of the SNP molecular marker is as shown in SEQID NO:1, or SEQID NO:2 or SEQID NO:3, C/T mutation, C/Tmutation and T/C mutation respectively exist at 327bp of the sequences as shown in the SEQID NO:1, the SEQID NO:2 and the SEQID NO:3, and the mutation of alleles cause polymorphism of Tsp45I-RFLP. Through the molecular marker, the analysis associated with the propagation property of the boars is performed in landrace and large white pigs. The invention also discloses a typing detection method of the molecular marker. A new marker is provided for the propagation property of the boars, and the molecular marker can be used for evaluating and detecting the propagation property of the boars and heredity improvement.