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238 results about "Purebred" patented technology

Purebreds, also called purebreeds, are cultivated varieties or cultivars of an animal species, achieved through the process of selective breeding. When the lineage of a purebred animal is recorded, that animal is said to be pedigreed.

Production method for high-density pure arbuscular mycorrhizal fungal spore

The invention discloses a production method for high-density pure arbuscular mycorrhizal fungal spore, which belongs to the technical field of microorganism culturing, and comprises the following technical steps of: 1) culturing carrot root tissues converted from agrobacterium rhizogenes plasmid DNA; 2) culturing the carrot root tissues converted from agrobacterium rhizogenes plasmid DNA and glomus intraradices together in an improved synthetic culture medium; 3) taking a culture containing the root, hypha and spore infected by the arbuscular mycorrhizal fungus and transplanting the culture to a special culture box for culturing; 4) collecting the spore and mycelium; and 5) storing the arbuscular mycorrhizal fungal spore. Compared with the prior art, the production method for high-density pure arbuscular mycorrhizal fungal spore has the advantages that: 1, the culture medium is an asepsis environment with low cost, which not only can meet the growing of a host plant, but also is suitable for the growing of AM epiphyte; 2, the nutrition requirements for the growing of the host plant are low and the host plant can grow and be cultured on the synthetic culture medium; 3, the spore cultured by the method is a non-pollution pure culture; and 4, the produced spore is stored in liquids and the activity can be maintained by above 90 percent.
Owner:ZHEJIANG NORMAL UNIVERSITY

Method for breeding high-quality mite-resistant bee species

The invention discloses a method for breeding a high-quality mite-resistant bee species. The method is characterized by comprising the following steps of: (1) selecting breeding materials: screening a 'Jiangshan No.2' Italian bee with a high royal jelly yield, a French pure-bred Italian bee with excellent honey making performance and a healthy local Chinese bee species with excellent mite resistance for serving as an introduction object according to series indexes such as mite resistance, productivity, vitality and the like; (2) breeding a mite-resistant bee species: breeding a breeding male bee from Jiangshan No.2, breeding a Jiangshan No.2 virgin queen bee according to the requirements of bee biology and genetic breeding characteristic, and performing artificial fertilization hybridization with the bred breeding male bee to form a Jiangshan No.2 bee species with high production performance and disease resistance for serving as a male parent, wherein a pure-bred Italian bee with excellent honey making performance introduced from France serves as a female parent; and (3) checking and cutting a French Italian bee drone pupae every 7 days from breeding in spring till the end of breeding. The harm of bee mites to bee colonies can be avoided for beekeepers, the reproduction speed of the bee colonies is increased, and the yields and qualities of bee products are improved.
Owner:LANXI HONGXIANG BIO TECH

Method for cultivating seedlings of purebred lycium ruthenicum with high survival rate

The invention relates to a method for cultivating seedlings of purebred lycium ruthenicum with a high survival rate. The problem that the method for cultivating the seedlings of the purebred lycium ruthenicum with the high survival rate does not exist at present is solved. The method comprises the steps of firstly, treating seeds, secondly, selecting a seedling cultivation field and setting up a seedbed, thirdly, treating a matrix, fourthly, sowing the seeds and cultivating the seedlings, and fifthly planting the seedlings and conducting transplanting. According to the method for cultivating the seedlings, genes of the lycium ruthenicum are not changed, the aim that growth is promoted is finally achieved by cutting the seedlings to shorten the seedling slowing time when the seedlings are transplanted, and the two methods of seedling cutting, growth promotion and alternating seedling hardening are combined to be used for enabling the seedling quality to be high and the transplanting survival rate to be high. Before the seedlings are transplanted, the percent of pass of the planting seedbed can be 96.1%, the survival rate of the transplanted seedlings can be 95.2%, any addition agent is not required, the invested cost is low, the method is easy to master, the technology is convenient to popularize, and the method is particularly suitable for large-scale seedling cultivation of the lycium ruthenicum and can be applied to artificial seedling cultivation of plants in the desert area.
Owner:薛军

Interspecific hybridization and propagation method for deep sea scallops and chlamys farreri

The invention discloses an interspecific hybridization and propagation method for deep sea scallops and chlamys farreri. The interspecific hybridization and propagation method comprises the following steps: a first step of breeding parent scallops: selecting deep sea scallops and chlamys farreri, separating the male and the female of the two scallops respectively, promoting maturity according to a normal parent scallop breeding method and using the mature scallops for cross experiment after synchronously maturing the mature scallops; a second step of laying eggs and performing hybridization: inducing spawning of the male and female groups of the two scallops respectively, fertilizing obtained eggs with conspecific or heterogeneous sperms respectively to obtain pure-bred self-bred progenies of the deep sea scallops and chlamys farreri as well as two hybrid fertilized eggs of female deep sea scallops and male chlamys farreri, female chlamys farreri and male deep sea scallops; hatching the obtained fertilized eggs at 13 to 14 DEG C, observing and measuring the proportion of D type larvas at regular times after the D type larvas appear until the maximum hatching rate is reached; a third step of cultivating scallops, wherein at a larval phase, the average shell height growth speed of the hybrid scallops is 6 to 6.3m per day, and the shell height, the shell length, the shell width and the weight are increased in a cultivating phase. According to the interspecific hybridization and propagation method disclosed by the invention, the deep sea scallops are effectively utilized through interspecific hybridization, so that the scallop breeding species are increased.
Owner:DALIAN CHANGHAI ZHENLU AQUATIC PROD

Method for breeding high-quality anti-mite bee variety

The invention discloses a method for breeding a high-quality anti-mite bee variety. The method is characterized by comprising the following steps of: 1) selecting breeding materials, wherein 'jiangshan No.2' Italian bees with relatively high royal jelly yield, France pure Italian bees with high honey production performance and healthy local Chinese bees with high anti-mite performance are selected as species introduction objects according to a series of indexes such as anti-mite performance, productivity and vitality; 2) breeding the anti-mite bee variety, wherein breeding drones are selected from the jiangshan No.2, selecting a bred jiangshan No.2 virgin queen bee according to the requirements of honey bee biology and inheritance breeding characteristics, and performing the artificial fertilization and cross breeding between the breeding drones and the bred jiangshan No.2 virgin queen bee so as to obtain a jiangshan No.2 bee variety with high production performance and disease resistance, using the jiangshan No.2 bee variety as male parents and using the pure Italian bees which are introduced from France and have high honey production performance as female parents; and 3) since breeding starts in spring, detecting and cutting off pupae of the France Italian bees every other 7 days till the breeding ends. The method can solve the problem that bee colonies are jeopardized by bee mites for beekeepers and improve breeding speed of the bee colonies and the yield and the quality of bee products.
Owner:LANXI HONGXIANG BIO TECH +1

Recessive white feather Beijing fatty chicken breeding method

The invention discloses a recessive white feather Beijing fatty chicken breeding method. The recessive white feather Beijing fatty chicken breeding method comprises the following steps: firstly, hybridizing purebred Beijing fatty female chickens with recessive white feather male chickens to obtain an F1 generation, secondly, crossing the F1 generation to obtain an F2 generation, thirdly, selecting fine quality white feather female chickens from the F2 generation to backcross the fine quality white feather female chickens with the purebred Beijing fatty male chickens to obtain an F3 generation, fourthly, crossing the F3 generation lou to obtain an F4 generation lou, fifthly, selecting a white feather crested F4 sub lou from the F4 generation lou and conducting crossing to obtain an F5 generation crested white feather Beijing fatty chicken sub lou, meanwhile, selecting a white feather bearded F'4 sub lou from the F4 generation lou and conducting crossing to obtain an F'5 generation bearded white feather Beijing fatty chicken sub lou, sixthly, conducting reciprocal hybridization on the F5 generation sub lou with the F'5 generation sub lou to obtain an F6 generation lou, seventhly, picking white feather chickens with all crested, breaded, feather-legged and five-toed features, persevering breeds of the picked chickens and conducting crossing to obtain an F7 generation, eighthly, close-breeding the F7 generation to obtain recessive white feather Beijing fatty chickens. The recessive white feather Beijing fatty chickens are high in growth speed and good in meat quality.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES

Method for cultivating green-shell-egg black-feather black-bone chicken

The invention provides a method for cultivating green-shell-egg black-feather black-bone chicken. The method includes the following steps that 1, purebred Dongxiang black-feather green-shell egg chicken serve as female parents, purebred Taihe silky black-bone chicken serve as male parents, the female parents and the male parents are hybridized to generate F1 generations, full-black male and female individuals with the full-black feathers and the black-color characteristics in the F1 generations are selected and remained; 2, the male and female individuals selected and remained in the step 1 are transversely mated and propagated, and individual recording and family breeding are permitted; 3, according to the compatriot selection method, the family selection method and the individual selection method, black-feather, black-color-characteristic and full-black hens generating green shell eggs at the same time are selected and remained in F2 generations generated in the step 2, and black-feather, black-color-characteristic and full-black cocks capable of allowing the hens to generate green shell eggs are selected and remained in the F2 generations; 4, the male and female individuals selected and remained in the step 3 are transversely mated and propagated and bred for 5 generations to 6 generations, and black-feather black-bone chicken generating green shell eggs are cultivated.
Owner:湖南文妹子现代农业科技生态园有限公司

Traditional Chinese medicine chicken feed and production method of black bone chicken egg with effects of regulating internal secretion of human body and removing spots and beautifying and protecting health

The invention discloses a production method of a traditional Chinese medicine chicken feed, which comprises the steps of: 1, preparing a base feed; 2, preparing Chinese medicinal herb powder or concentrated solution; and 3, adding the Chinese medicinal herb powder or concentrated solution into the base feed and uniformly stirring. Meanwhile, the invention also discloses a production method of a black bone chicken egg with effects of regulating internal secretion of human body and removing spots and beautifying and protecting health, which comprises the following steps of: 1, selecting a healthy and purebred Taihe original black bone chicken seedling, feeding with a common feed for 110-120 days to ensure that the black bone chicken seedling grows to a healthy expectant layer; 2, feeding with the traditional Chinese medicine chicken feed for three times of morning, noon and night, wherein the feeding amount of each chicken every day is 0.1-0.15kg; and 3, feeding until about 450 days andthen stopping feeding with the traditional Chinese medicine chicken feed, and discarding the chicken. The black bone chicken egg produced by adopting the method has effective components of rich protein and multiple amino acids and the like, especially rich in cysteine, and has the effects of regulating internal secretion of human body and removing spots and beautifying after eaten by people.
Owner:深圳市生物谷科技投资有限公司

Method for select-breeding anti-virosis Bombyx mori varieties by utilizing Thailand Bombyx mori disease-resistant gene

The invention discloses a method for select-breeding anti-virosis Bombyx mori varieties by using Thailand Bombyx mori disease-resistant gene. The method comprises the steps of: selecting the present Bombyx mori varieties of 871 and 872 used both in spring and autumn in China as female parent and using the introduced Thailand tropical disease-resistant variety NL as male parent; respectively hybridizing and leading-in the disease-resistance gene; hybridizing second generation and third generation silkworm and respectively adopting mixed batches rearing for breeding; hybridizing each generation beginning from a second age and screening by adopting 'population feeding' NPV (nuclear polyhedrosis virus) and a method with the concentration of virus feeding 'increasing age by age in a gradient way'; taking the Bombyx mori varieties 871 and 872 as recurrent parent, respectively adopting reserve seed antivirus silkworm individual to backcross, using a method of the combination of 'backcrossing introduction' and 'generation directed virus feeding screening breeding' to obtain an NL pure and mild system with comparatively stable genetic character by a plurality of backcrosses and virus feeding screening. In the process of select-breeding, various economic characters and production properties of the system are consolidated and improved and the objects of pure breeds of 871NL and 872NL are bred. The obtained antivirus Bombyx mori variety has good economic characters, is sturdy and easy to keep.
Owner:ANHUI AGRICULTURAL UNIVERSITY

Preparation method of hybrid hydrolase formulation for promoting methane yield from fermentation

InactiveCN1884509AIncrease fermentation gas productionPromote degradationHydrolasesMicroorganismsBiotechnologyMethane yield
The method for preparation of hydrolytic enzyme preparation used for improving gas-producing rate of marsh gas fermentation belongs to preparation of supplementary exoenzyme used for marsh gas fermentation. The invention is to provide a method for preparation of hydrolytic enzyme preparation used for improving gas-producing rate of marsh gas fermentation, which has the production bacteria with the following enzymatic vitality index: selecting wheat bran and rice bran as mixed culture medium, obtaining mixed bran seeds by pedigree breed two-stage enlargement culture of the bacteria with solid method, fermenting for 3-4 days at 28 DEG after evenly mixed, sampling and analyzing every enzymatic vitality index, adding commodity enzyme preparation to reach enzymatic vitality index, and airing until the humidity is lower than 5%. The enzymatic vitality index of the prolease in the said mixed bran seeds is not more than 2000+-5% mug tyrosine / g / min. The bacteria can be obtained by bacteria and fungus separated from different environment. The test proves that when hydrolytic enzyme preparation is input into the methane tank fermentation raw materials, the rate of dissolution and degradation of the marsh gas fermentation raw materials can be improve, and the gas-producing rate of marsh gas fermentation, TS and VS gas-production potential and methane content in marsh gas can be increased.
Owner:YUNNAN NORMAL UNIV

North China fermented soya bean series

InactiveCN101278720AThe taste is strong, mellow and uniqueUnique tasteFood preparationSnow moldFood flavor
The invention relates to a food for people living requirement, in particular to a north China lobster sauce series and a manufacturing method thereof, which is made from soybeans, peeled peanuts, almonds, fresh gingers, green peppers, various diced melons and vegetables, cooked diced meat (such as pork, beef, chicken, fish and shrimp meat, etc.), salty, sour and sweet flavorings, flour and pure mildew strain. When being manufactured, the soybeans are soaked and cooked; the flour with strain is mixed with the soybeans; natural bacterial colonies and the strain are compounded for producing leaven, the produced leaven is dried, and then spore mycelium is scoured off, and the natural bacterial colonies and the strain are poured into a jar; the mashed fresh gingers, the green peppers in small cluster and the cooked peanuts and the almonds are put into the jar, water and slat are added to be mixed uniformly, and the jar is sealed and put in a ferment room to be fermented under constant temperature for more than two months; after the jar is unsealed, a product is poured out and portioned, the sour and sweet flavorings, the diced melons and vegetables and the cooked diced meat are respectively added. The product is packed by soft and hard cans and is sterilized by high temperature, and the north China lobster sauce series with low salt, intense and delicious taste and multiple flavors are obtained.
Owner:李左棠

Method for breeding pig breeds of Meiling black pigs

The invention discloses a method for breeding pig breeds of Meiling black pigs. The method comprises the following steps that (1) excellent piglets are selected as reserved backup pigs on the 20th day after the birth of the piglets; (2) the secondary optimization screening is carried out after fourth month of the reserved backup pig selection, and high-quality stock pig breeds are selected; (3) when the body weight of the selected high-quality stock pig breeds is increased to 50kg, the selection remaining and the elimination are carried out by utilizing the integral selection indexes and referring to the genealogical ancestry and the somatotype appearance according to the individual and compatriot performance test results, and backup pig groups are selected and formed; (4) male pigs and female pigs in the backup pig groups can mate according to requirements, and pigs with poor growth and development conditions, genetic diseases and breeding difficulty are weeded out. The method for breeding pig breeds of Meiling black pigs is set out according to the actual characteristics of the pig breeds of the Meiling black pigs. When the method for breeding pig breeds of Meiling black pigs is adopted, the purebred pig breeds of Meiling black pigs with good properties and stable hereditary feature can be selected, and the social benefits and the economic benefits are obvious.
Owner:韦露

Method of making mature vinegar through solid state fermentation of intensified multi-microorganism mouldy bran and yeast for making hard liquor as mould starter

ActiveCN109486643AStrains with a wide range of enzymesSimple production processVinegar preparationSpore germinationChaff
The invention discloses a method of making mature vinegar through solid state fermentation of intensified multi-microorganism mouldy bran and yeast for making hard liquor as a mould starter. The method comprises the following steps of: step (1). firstly mixing the yeast for making hard liquor as the mould starter with a bran culture medium subjected to steaming treatment for accumulation, promoting the spore germination, then inoculating a culture medium with bran, rice hull, soybean meal, graham flour and water as raw materials, and producing the intensified mouldy bran through the starter propagation by mechanical ventilation; and step (2). carrying out alcoholic fermentation and high temperature solid acetic fermentation by using sorghum and bran as main raw materials, chaff and rice hull as auxiliary materials, and traditional yeast for making hard liquor, the intensified multi-microorganism mouldy bran and pure mouldy bran as leavening agents, smoking unstrained liquor, pouring vinegar and ageing to form the mature vinegar. The intensified multi-microorganism mouldy bran is simple in production process, is the same as the pure mouldy bran in terms of place and equipment and the like for production, and can be produced without additional equipment; and the production cycle of the intensified multi-microorganism mouldy bran is greatly shortened than that of the yeast for making hard liquor.
Owner:SHANXI ZILIN VINEGAR
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