16 results about "Purebred" patented technology

The present application relates to the field of molecular markers and animal genetic breeding technology, and particularly relates to a SNP marker affecting pig purine base content and application thereof. Based on three test pig populations of American purebred Landrace, American purebred Large White and American purebred Duroc, the present application uses pig whole genome resequencing and GWAS analysis to research and determine 15 SNP markers significantly affecting pig purine base content. By selecting the advantageous alleles of the above SNP markers, the frequency of the advantageous alleles can be increased generation by generation, the corresponding purine content can be reduced or increased, the progress of pig genetic improvement can be accelerated, and the economic benefits of pig breeding can be effectively improved.

The invention provides a Taizhou goose germplasm analysis and identification method based on whole genome re-sequencing. The Taizhou goose germplasm analysis and identification method is used for accurately identifying and identifying different varieties of geese. Rapid identification and identity traceability of local goose germplasm are realized by constructing a local purebred goose germplasm reference library and combining with a lineage analysis and molecular identity card technology.

The application discloses a kind of integrated hybrid information purebred genome prediction method, belong to animal genetic breeding and propagation technical field.The population genetic dataset comprising purebred population A, B and hybrid population CB is constructed, and the population genetic differentiation is verified by principal component analysis and genetic differentiation index;After the genotypes of purebred and hybrid population CB are phased, the ancestral information inference method is used to determine the origin information of each allele and process missing sites;Based on the origin information of allele, additive effect partial kinship correlation matrix and dominant effect partial kinship correlation matrix are constructed;The above matrix is integrated into three-trait mixed linear model, forming the prediction model that simultaneously incorporates purebred and hybrid population genetic information;The application distinguishes the breed origin of allele and quantifies its additive and dominant effect, significantly improves the prediction accuracy of purebred genome breeding value, is suitable for the traits difficult to directly determine in purebred population, and provides reliable technical support for animal breeding.

This invention discloses a method for accurately assessing the germplasm purity of Fujian oysters, comprising the following steps: Step 1, acquiring high-coverage whole-genome resequencing data of the oyster individual to be tested; Step 2, comparing the resequencing data with the Fujian oyster reference genome and identifying the nucleotide base types of the individual to be tested; Step 3, assigning a score to each specific single nucleotide polymorphism site and accumulating the scores of all sites to obtain a total score; Step 4, calculating the Fujian oyster germplasm purity value of the individual to be tested based on the total score. This invention utilizes high-coverage genome resequencing to screen for single nucleotide polymorphism sites throughout the Fujian oyster genome and compares them with the genome sequence of Pacific oyster to identify highly reliable Fujian oyster genome-specific nucleotide bases, constructing a purebred Fujian oyster genome sequence site set, and achieving efficient and accurate quantitative assessment of Fujian oyster germplasm purity by calculating the percentage of purebred sites in the Fujian oyster genome.

This invention relates to the fields of molecular markers and animal genetic breeding technology, and particularly to a SNP marker affecting the total flavor nucleotide content in pigs and its application. Based on three experimental pig populations—purebred American Landrace, purebred American Large White, and purebred American Duroc—this invention employs whole-genome resequencing and GWAS analysis to study and identify nine SNP markers associated with total flavor nucleotides. By optimizing the dominant alleles of these SNPs, the frequency of dominant alleles can be increased generation by generation, thereby increasing the total flavor nucleotide content, improving pork quality, accelerating the progress of pig genetic improvement, and effectively improving the economic benefits of pig breeding.

The invention relates to the technical field of biology, in particular to a primer group for identifying Hangzhou pig breeds, a detection method and application. Four specific SNP molecular markers SNP1-SNP4 are obtained through whole genome screening, the upstream and downstream sequences of the molecular markers are shown as SEQ ID NO.1-4, four pairs of specific PCR primers are designed and developed based on the sites, and the primer group and the detection method are applied to the identification of Hangzhou pig breeds. The nucleotide sequence of the gene is as shown in SEQ ID NO.5-12. And when the genotypes of the to-be-detected sample at the SNP1-SNP4 sites are CC, CC, TT and CC respectively, determining that the to-be-detected sample is a Hangzhou pig purebred individual. Compared with a traditional phenotype identification method, the molecular marker detection system established by the invention has the advantages of high detection efficiency, good accuracy and flux. The technical scheme provided by the invention provides important technical support for germplasm resource protection, variety purity identification and genetic improvement of Hangzhou pigs, and has remarkable application value in the field of livestock breeding.

The application discloses a high-quality pig creating and breeding method, which comprises the following steps: 1) a certain blood structure of purebred Luchuan pigs is screened through molecular pedigree construction based on genome information, and the screened Luchuan pigs are used as female parents to cross with Duroc boars to generate Duroc-Luchuan crossbred pigs; 2) the Duroc-Luchuan crossbred pigs are used as female parents to cross with Barcshires boars to generate Bar-Duroc-Luchuan crossbred pigs; 3) the Bar-Duroc-Luchuan crossbred pigs are used as female parents to cross with Duroc boars to generate Duroc-Bar-Duroc-Luchuan crossbred pigs; 4) based on the Duroc-Bar-Duroc-Luchuan crossbred pigs, male and female pigs meeting the breeding target are selected according to a selection index to realize horizontal cross fixation, and an excellent horizontal cross offspring is selected to form a breeding core group; and 5) the core group in the step 4) is used as a zero generation group, a group succession breeding method is adopted, whole genome selection and genome prediction and the like are used to estimate a genome breeding value of breeding pigs and predict a phenotype, and precise intelligent breeding selection and matching are realized.

The application discloses a breeding method of a green-shell egg producing black-bone chicken with seven-features and yellow-feather and dual-purpose, which comprises the following steps: taking yellow-feather line Xuefeng black-bone chicken as a KH line material, and then performing subline pure line breeding to obtain yellow-feather male chickens as a first male parent; taking pure line yellow-feather Xuefeng black-bone chicken as an H line material, and then performing subline pure line breeding to obtain yellow-feather female chickens as a first female parent; performing cross breeding of the first male parent and the first female parent to obtain a terminal male parent F1; taking yellow-feather pure line green-shell egg producing Xuefeng black-bone chicken as a LH line material, and then performing subline pure line breeding to obtain yellow-feather green-shell egg producing female chickens as a terminal female parent; and performing cross breeding of the terminal male parent and the terminal female parent to obtain a commercial generation green-shell egg producing black-bone chicken with seven-features and yellow-feather and dual-purpose. The black-bone chicken obtained by the breeding method has the basic features, flavor and nutritional value of the local pure breed Xuefeng black-bone chicken, can produce green-shell eggs, has a high egg production rate, has a fast growth speed, has good meat performance, has a high survival rate, and the like.

The invention relates to the technical field of genome information analysis, in particular to a local pig optimal heterosis prediction method based on genome information. According to the method, pure parents with clear genetic backgrounds are accurately identified and screened out by utilizing high-density SNP marker information covering a whole genome, a reference population is constructed, large-scale hybridization propagation is simulated, structured virtual population data is formed, and multi-character phenotype data of filial generations are standardized and weighted, so that the genetic backgrounds of the filial generations are optimized, and the genetic backgrounds of the filial generations are optimized. The method comprises the following steps of: dividing a plurality of characteristic groups such as a production performance type and a reproductive performance type, and analyzing the correlation between phenotypic deviation and genotype in each group, so as to separate and quantify dominant effect caused by heterozygous genotype and recessive effect caused by homozygous genotype, construct explicit and recessive weight parameters, and determine the genetic relationship between the dominant effect and the recessive effect; finally, the non-additive effect parameters are superposed to a traditional additive breeding value model, direct quantitative prediction of heterosis is achieved, and therefore the accuracy and efficiency of hybrid combination matching are greatly improved.

This invention relates to the fields of molecular markers and animal genetic breeding technology, and particularly to a SNP marker that affects porcine inosine content and its application. Based on purebred American Landrace, purebred American Large White, and purebred American Duroc pigs, this invention studies and identifies 10 SNP molecular markers closely related to porcine inosine content. By optimizing the dominant alleles of these SNPs, the frequency of dominant alleles can be increased generation by generation, reducing inosine content, accelerating the progress of pig genetic improvement, and thus effectively improving the economic benefits of pig breeding.

The invention discloses a computer device and method for predicting animal sample ancestor bloodline and variety purity and application. Characteristic SNP marker sites for predicting pig varieties are obtained from local pig varieties through screening, a prediction model for predicting the varieties of to-be-detected pigs is constructed by using a machine algorithm on the basis of the sites, whether the to-be-detected pigs belong to known pig varieties or not can be predicted through the model, and for pigs predicted to belong to unknown varieties, the method has the advantages that the prediction accuracy is high, and the prediction cost is low. According to the method, a hybrid population data set and an unknown variety data set are constructed, and a classifier model for predicting whether an unknown variety pig belongs to an out-of-library variety or a hybrid individual is obtained through training. According to the method disclosed by the invention, the correlativity between different markers is considered, and the purebred and bloodline mixing of the animal to be detected can be accurately judged by using the computer device and the method disclosed by the invention.

The invention relates to the technical field of medicinal breeding of special insects, in particular to a method for ecologically and cyclically domesticating high-quality maggot and a domesticated population thereof, and the method comprises the following steps: seed source acquisition and identification: acquiring chrysomyia megacephala adults, performing purebred identification and health detection, and pre-screening an initial seed source; hatching eggs of the obtained provenance in an artificial environment, feeding larvae of 1-3 years old, applying primary environmental stress, and screening individuals; putting imagoes into the natural environment after emergence of the individuals, and recovering spawning blocks; the recovered egg masses are hatched and fed with larvae of 1-3 years old, composite environmental stress is applied, and individuals with tolerance reaching the standard are screened out; after the individuals emerge adults, putting the adults into a complex natural environment, and recovering the spawn blocks; the recovered egg masses are hatched and fed with larvae of 1-3 years old, ultimate environmental stress is applied, and domesticated populations with solidified characters are obtained through screening. Through three-generation directional domestication, artificial stress, natural energizing and fresh fish waste utilization, the effects of pure maggot variety, strong stress resistance, high activity and stable breeding are achieved.

This invention relates to the fields of molecular markers and animal genetic breeding technology, and particularly to a SNP marker that affects AMP content in pigs and its application. Based on three experimental pig populations—purebred American Landrace, purebred American Large White, and purebred American Duroc—this invention uses whole-genome resequencing and GWAS analysis to identify 14 SNP molecular markers significantly associated with AMP content. By optimizing the dominant alleles of these SNPs, the frequency of dominant alleles can be increased generation by generation, thereby increasing AMP content, accelerating the progress of pig genetic improvement, and effectively improving the economic benefits of pig breeding.

This invention relates to the fields of molecular markers and animal genetic breeding technology, and particularly to a SNP marker that affects the IMP or GMP content in pigs and its application. Based on three experimental pig populations—purebred American Landrace, purebred American Large White, and purebred American Duroc—this invention employs whole-genome resequencing and GWAS analysis to study and identify 15 SNP markers associated with IMP or GMP content. By optimizing the dominant alleles of these SNPs, the frequency of dominant alleles can be increased generation by generation, thereby increasing IMP or GMP content, accelerating the progress of pig genetic improvement, and effectively improving the economic benefits of pig breeding.

The invention discloses a method for accurately evaluating the germplasm purity of crassostrea gigas, and relates to the technical field of aquatic genetic breeding biology, the core of the method is to construct an index based on an NCBI (National Center of Biotechnology Information) crassostrea gigas genome sequence by virtue of a whole genome re-sequencing technology, collect 150 crassostrea gigas and 150 Fujian oysters in a natural population, extract mantle DNA (Deoxyribose Nucleic Acid) for sequencing, screen out 635 specific SNP (Single Nucleotide Polymorphism) sites, and obtain the purity of the crassostrea gigas. The method comprises the following steps: constructing a pure variety feature sequence, sequencing a to-be-detected sample, comparing two detection sequences of 635 sites with the feature sequence, and calculating a consistency average value according to the same base number / sequence length * 100%, namely the germplasm purity value. The method fills the blank that quantitative evaluation cannot be carried out in the prior art, is high in precision and reliability, and can provide key technical support for crassostrea gigas breeding improvement, germplasm protection, industrial standardization and the like.

This invention relates to the fields of molecular markers and animal genetic breeding technology, and particularly to a SNP marker affecting UMP content in pigs and its application. Using whole-genome resequencing and GWAS analysis, this invention studied purebred Landrace, Large White, and Duroc pigs as research subjects, identifying seven SNP markers associated with UMP content in pigs. By optimizing the dominant alleles of these SNPs, the frequency of dominant alleles can be increased generation by generation, thereby increasing UMP content, accelerating the progress of pig genetic improvement, and effectively improving the economic benefits of pig breeding.