117 results about "Epinephelus" patented technology

The invention relates to an industrial artificial breeding method of epinephelus lanceolatus. The breeding method comprises the following steps of: (1) preparing a breeding environment condition; (2) doing breeding preparation works; (3) controlling the spawn planting density; (4) carrying out feed management; and (5) controlling the water quality. The breeding method of the epinephelus lanceolatus has the advantages of quick fry growth, high survival rate, small influence of exterior natural conditions, and the like.

The invention relates to a method for industrialized seedling raising of saddle grouper, which is suitable for saddle grouper (Epinepheluslanceolatus). The steps taken by the method include: 1. seedling raising workshop, seedling pond and seedling water treatment; 2. preparation before seedling raising; 3. Putting fertilized eggs or larvae of saddle grouper; 4. Water quality control of nursery water; 5. Bait and feeding methods. The method of the present invention carries out industrialized seedling raising of saddle grouper, and has the advantages of stable water quality conditions for seedling raising, high seedling output per unit of water, neat specifications of the bred fish fingerlings, no use of any antibacterial drugs, and no additional preparation of larva opening bait during the seedling raising process 1. The nursery does not need to cultivate single-cell algae alone, and the whole process of seedling cultivation does not suck the bottom, which greatly simplifies the operation process.

A microgranular bait for the fry of Epinephelus is prepared from crude protein, crude fat, phosphatide, ash, n-3HUFA, DHA / EPA, DHA, and EPA according to a certain proportion through enzymolyzing by proteinase, further adding antioxidizing agent, filtering, and spray drying. Its advantages are rich nutrients, and low settling speed.

The invention discloses a method for culturing Epinephelus coioides at a low salt degree, and relates to a culturing method for aquatic livestock, in particular to a method for culturing the Epinephelus coioides. The method comprises the following steps of: desalting sea water for culturing healthy Epinephelus coioides at a rate of decreasing 0.1 to 0.2 percent of salt degree per 24 hours; and when the salt degree is desalted to 0.8 percent, putting the Epinephelus coioides into a pond in which the salt degree of the water body is 0.8 percent for culturing, wherein the salt degree of the sea water is greater than 2.0 percent. By manually desalting and domesticating gradually, a pond culturing mode of the Epinephelus coioides is optimized so that the survival rate reaches over 70 percent. The yield of the Epinephelus coioides in each pot is over 150 kilograms; and the yield of the Epinephelus coioides of each mu of the pond is 500 kilograms.

The invention discloses a compound granulated feed for young epinephelus, which is suitable for feeding young epinephelus, i.e., the young epinephelus with the weight ranging from 50g to 500g. The compound granulated feed comprises the following components by weight percent: fish meal, flour, extruded soybeans, bean pulps, wheat gluten flour, fish oil, beer yeast, mineral substances, monocalcium phosphate, choline chloride, seaweed powders, an emulsifying agent, various vitamins and the like. The compound feed provided by the invention has the advantages that various nutrients are provided: the compound granulated feed is rich in various necessary proteins, trace elements, special mineral substances, vitamins and the like, so that the requirement of the young epinephelus for various nutritional ingredients can be met, and the conversion ratio of the feed is high; the compound granulated feed has good stability in water and little pollutes a cultivation water body: natural raw materials such as the wheat gluten flour and the like which are beneficial to the formation and the stabilization of a feed product are added, so that the compound granulated feed can be soaked in water for over 3h without dispersing, and the pollution to the cultivation water body is reduced; and the emulsifying agent is additionally provided so that the utilization efficiency of the young epinephelus to fat is improved.

The invention relates to an artificial forced mature method to girth rockfishes, characterized in that adding yeast cell wall, mixture with abio salinous, phytic acid enzyme and methyl testis ketone, etc, into bait to force male parent fish to mature in web box on sea and enhanced feed in doors' pool, then by injecting DOM and ecology stimulus forces parent fish gonad to mature and oviposit naturally. This invention can achieve artificial control on parent fish sex of girth rockfishes with at least 70% triumph rate on sex adjustment. Meanwhile young functional male fish gained can be also used to production, which makes more than 50% parent fish come to sex mature and oviposit naturally and obtains mass high class gamete, thereby improve utilization rate of parent fish and productive capacity.

The invention discloses a compound granulated feed for adult epinephelus, which is suitable for feeding adult epinephelus, i.e., the adult epinephelus with the weight more than 500g. The compound granulated feed comprises the following components by weight percent: fish meal, flour, extruded soybeans, bean pulps, wheat gluten flour, fish oil, beer yeast, mineral substances, monocalcium phosphate, choline chloride, seaweed powder, an emulsifying agent, various vitamins and the like. The compound feed provided by the invention has the advantages that various nutrients are provided: the compound granulated feed is rich in various necessary proteins, trace elements, special mineral substances, vitamins and the like, so that the requirement of the adult epinephelus for various nutritional ingredients can be met, and the conversion ratio of the feed is high; the compound granulated feed has good stability in water and little pollutes a cultivation water body: natural raw materials such as the wheat gluten flour and the like which are beneficial to the formation and the stabilization of a feed product are added, so that the product can be soaked in water for over 3h without dispersing, and the pollution to the cultivation water body is reduced; and the emulsifying agent is additionally provided so that the utilization efficiency of the adult epinephelus to fat is improved.

The invention discloses a method for cyclically cultivating Epinephelus coioides with artificial seawater indoors, which comprises the following steps of preparation of artificial seawater, preparation of cultivation water, selection of fry, regulation and control of water quality, selection of feed, daily sewage suction and water change, and cleaness maintenance of the bottom and walls of a pond. The method is efficient and environment-friendly; Epinephelus coioides obtained according to the method can be high in quality and yield, and healthy, in addition the cultivating survival rate is high, and the feed coefficient is low.

The invention discloses an SNP (single nucleotide polymorphism) marker and application thereof. The SNP marker is the 381st base T or C from the 5' terminal of a nucleotide sequence as shown in the SEQ ID NO:1. The SNP marker is closely related to the weight trait of epinephelus coioides, and can be effectively used for molecular mark assisted breeding of epinephelus coioides.

The present invention provides an industrial aquaculture illumination system of promoting the epinephelus fry growth and improving a survival rate and an application thereof. The illumination system comprises a composite LED lamp set composed of the cold white, green and blue monochromatic LED light emitting chips, a light source control system and a waterproof shell, wherein the light source control system comprises a power supply module and a circuit control module and can control the illumination intensity and illumination time of the composite LED lamp set, and the waterproof shell uses an anticorrosion material and carries out the waterproof processing by a sealant. The system and application of the present invention are developed and set according to the hobbies of the epinephelus juvenile fishes to the specific spectrum, the light intensity and the illumination cycle, can satisfy the demands of the industrial aquaculture epinephelus juvenile fishes on the light colors, the light intensity and the light cycle, promote the healthy growth of the epinephelus juvenile fishes and improve the survival rate, and further enable the economic benefits of the epinephelus industrial aquaculture to be improved.

The present invention discloses one kind of Epinephelus coioide culturing method and aims at provides Epinephelus coioide culturing method with high efficiency, no environmental pollution and adult fish eating safety. The method includes constructing indoor culture pond and biological filtering pond, throwing Epinephelus coioide fry of 50 g weight into the culture pond and raising with bait while controlling the conditions essential for Epinephelus coioide to grow. The indoor raising method has no escape of Epinephelus coioide, fast growth and high raising efficiency. The communicated indoor culture pond and biological filtering pond can ensure high water quality, less fish disease, no environmental pollution and adult fish eating safety. The technology of the present invention is applicable in culture of other fish.

The invention discloses an SNP marker relevant to ammonia endurance capacity of epinephelus coioides and the use of the SNP marker. The nucleotide sequence of the SNP marker is as shown in SEQ ID NO.1, and the 501st basic group from 5' end in the sequence is G or T. The SNP marker is used for molecular marker auxiliary breeding of the epinephelus coioides. The invention further provides a primer and reagent kit for detecting the SNP marker relevant to the ammonia endurance capacity of epinephelus coioides, and the use of the primer and the reagent kit to breeding of the epinephelus coioides. The invention also provides a method for detecting the ammonia endurance capacity of the epinephelus coioides through the SNP marker, the primer and the reagent kit. Through the adoption of the SNP marker disclosed by the invention, the ammonia endurance capacity of the epinephelus coioides can be detected quickly, efficiently and accurately, and further the SNP marker can be effectively used for molecular marker auxiliary breeding of the epinephelus coioides, so that short-time low-cost high-accuracy breeding of the epinephelus coioides of good varieties can be early realized in an auxiliary manner.

The invention discloses an epinephelus coioicles ecological feed formula and a preparation method. The epinephelus coioicles ecological feed is prepared from fish meal, tenebrio molitor powder, nereis powder, earthworm powder, silkworm chrysalis meal, shrimp head meal, blood meal, squid visceral powder, beer yeast, bean pulp, soybean phospholipid, alpha-starch, flour, alfalfa meal, moringa powder, macroalgae mixed powder, fish oil, shell mixed powder, dry ginger powder, choline chloride, composite multiple vitamin powder and a phytase preparation. The raw materials are weighed according the proportions, cleaned, dried, smashed and put into a vessel to be stirred to be uniform, granulation is conducted through fluidized bed spray, feed particles are cooled, subpackaged and sealed, and the target product is obtained. According to the epinephelus coioicles ecological feed, feeding is convenient, storing is convenient, the feed quality is high, the nutritional requirement of epinephelus coioicles is met, preparation is dry and thorough, long-term storing is convenient, the feed is not prone to deterioration, active substances in tenebrio molitor, nereides, silkworm chrysalises and fresh ginger can not be subjected to inactivation, the utilization rate of the epinephelus coioicles to the feed is increased, and the growth performance of the epinephelus coioicles is improved.

The invention belongs to the field of aquaculture and relates to a method for preventing and controlling prawn communicable diseases by Epinephelus coioids. The method comprises the steps of: releasing young prawns in high-salinity seawater, wherein the releasing amount is 80,000-10,0000 fish per mu (mu refers to a unit of area); when the prawn is cultivated to 0.3-0.7 gram, releasing the Epinephelus coioids into the seawater, wherein the weight of each Epinephelus coioids is 0.10-0.15 kg, and the releasing amount of the Epinephelus coioids is 15-20 fish per mu. Large amount of experiments prove that: the Epinephelus coioids about 0.10-0.15 kg in weight can eat ill and dead prawns in a culture pond, the probability of eating healthy prawns is very little due to the habit of the healthy prawns. According to the characteristic, the problem that the survival rate of the bred prawns is low because the healthy prawns are eaten is solved, and simultaneously, the prawn communicable diseases are effectively prevented and controlled.

The invention discloses a cloning vector containing a mature peptide gene GTHalpha or FSHbeta of an epinephelus coioids gonadotropin hormone, an eukaryotic expression vector, a recombinant strain, a recombinant protein and application of the recombinant protein to the preparation of a fish oxytocic. Epinephelus coioids belongs to a rare marine fish and has high economic value. The recombinant protein for recombining the epinephelus coioids gonadotropin hormone I is produced by adopting a gene-engineering technology and can be used for preparing the fish oxytocic with low preparation cost and remarkable effect.

The invention discloses an SNP (single nucleotide polymorphism) marker and application thereof. The SNP marker is the 251st base T or C from the 5' terminal of a nucleotide sequence as shown in the SEQ ID NO:1. The SNP marker is closely related to the overall length trait of epinephelus coioides, and can be effectively used for molecular mark assisted breeding of epinephelus coioides.

The invention relates to an epinephelus radiates female fish and epinephelus lanceolatus milter crossbreeding method, which comprises the steps of parent selection, parent water temperature regulation and control, parent nutrition enhancement, parent hasten parturition, artificial insemination and incubation; the epinephelus radiates female fish is warm middle-lower layer fish with large individual and high growth speed; the epinephelus lanceolatus fish is tropical and sub-tropical region warm water fish with high growth speed and high nutrition value; after the crossbreeding, the excellent later generations with high growth speed and wide temperature adaptation range can be obtained.

The invention discloses a compound feed with low content of fish meal for epinephelus coioides, and relates to the technical field of fish feeds. The compound feed consists of the following components in parts by weight: 12 to 18 parts of fish meal, 28 to 40 parts of casein, 7 to 10 parts of gelatin, 2 to 4 parts of fish oil, 2 to 4 parts of soybean oil, 1 to 3 parts of granulesten, 8 to 35 parts of corn starch, 1 to 2 parts of monocalcium phosphate, 0.2 to 0.6 part of compound vitamin premix, 0.2 to 0.6 part of compound trace element premix, 0.3 to 0.8 part of taurine, 1 to 2 parts of sodium alginate, 0.1 to 0.3 part of choline chloride, 2 to 12 parts of zeolite powder, and 2 to 12 parts of microcrystalline cellulose powder; the raw materials are crushed, screened, mixed and granulated, so as to prepare the compound feed for the epinephelus coioides. The compound feed has the advantages that the obtaining of raw materials is easy, the nutrients are balanced, the processing is simple and convenient, the usage ratio and amount of fish meal in the feed for the epinephelus coioide are reduced, the cost is low, and the compound feed is suitable for industrialized popularization.

A slow-releasing oxytocic for artificially inducing the maturation of epinephelus' sex gland and the ovulation or ejaculation by embedding it in body of epinephelus is prepared from luliberin like, 17 alpha-hydroxyprogesterone, cholesterin, cellulose and plant oil through proportional mixing and moulding.

The invention relates to a production method for cryptocaryon irritant prevention epinephelus coioides seedlings, and belongs to the field of disease resistance fry production, and specifically relates to the field of cryptocaryon irritant prevention fry production. A method of varying temperature infection and varying temperature culture is firstly used to produce disease resistance epinephelus coioides seedlings. Water temperature in infection and cultivation water temperature in 10 days after infection keep certain temperature difference, so as to enhance immune effect of cryptocaryon irritant live larva, to further combine with rotational culture. The method obtains cryptocaryon irritant prevention epinephelus coioides seedlings in a relatively long effective immune protection period. In the eighth month after immunity, the seedlings still have relative protection rate of 60%, and in the ninth month, the seedlings still have relative protection rate of 34%.

The invention discloses an artificial fry breeding method for epinephelus coioides. The breeding process includes the steps of roe laying and hatching, larva breeding, fry grading, and juvenile fish breeding. The method has the advantages that according to the development characteristics of feeding organs of epinephelus coioides larvae, a matched bait line for feeding oyster fertilized eggs, rotifers and artemia is formulated, the method is scientific and reasonable, and the growth speed of the larvae is high; the natural growth environment of the epinephelus coioides is simulated, the environment suitable for hatching of fertilized eggs of the epinephelus coioides and growth of the larvae is created, and the survival rate of the artificially bred epinephelus coioides is increased; in the larvae breeding stage, a chlorella solution containing active polypeptides is injected into pond water, so that the leukopoiesis capacity of the larvae is improved, the non-specific immunity of the larvae is enhanced, and the anti-stress capability of the larvae is improved.

The invention provides a preparation method and application of a polyclonal antibody against CCR12 of Epinephelus coioides. Currently, three XCR1-like chemokine receptors are discovered in fish, i.e., XCR1, XCR1L and CCR12, wherein XCR1L and CCR12 are only discovered in the genomes of fish, but little research is conducted on the functions of XCR1L and CCR12; and so far, in-vitro expression of CCR12 from fish has not been reported, no one has prepared a polyclonal antibody against CCR12 of Epinephelus coioides, and application of the polyclonal antibody against CCR12 has not been reported, either. To further reveal the migration or propagation of Epinephelus coioides CCR12 positive cells in tissue with parasitic infections and to identify the types of the CCR12 positive cells, a recombinant protein of the N-terminal region and three extracellular regions the CCR12 is expressed via a prokaryotic system and purified, and a rabbit anti-CCR12 recombinant protein polyclonal antibody is eventually prepared for the first time; and the antibody can specific recognize the CCR12 protein of Epinephelus coioides and mark cells in blood, so a foundation is laid for further marking of the CCR12 positive cells and research on parasitic infection resistance of the CCR12 positive cells.

The invention discloses a method for preventing and controlling shrimp communicable diseases through twice epinephelus coioids release. In the method, epinephelus coioids of two different size specifications are released in a prawn culture region twice, thus the prevention and control of the peneldshrimp communicable diseases can be realized, and the survival rate of prawns can be improved. By utilizing the method disclosed by the invention, shrimp diseases can be prevented from being spread and popularized among healthy shrimp clusters, the work load of artificially removing sick shrimps and died shrimps is reduced, the removing efficiency and the culture success ratio are improved, and greater economic benefits are brought to culturists.

The invention discloses an epinephelus coioides sex control gene Rspo1 and an application thereof. The cDNA (complementary deoxyribonucleic acid) sequence of the gene Rspo1 is as shown in SEQ (sequence) ID (identity) NO: 1, the total length is 756bp, an encoding protein of the gene Rspo1 has 251 amino acids in total, and the sequence is as shown in SEQ ID NO: 2. According to the method disclosed by the invention, a reverse transcription PCR (polymerase chain reaction) technology is utilized to clone the epinephelus coioides sex control gene Rspo1 from epinephelus coioides, and experimental results show that the Rspo1 possibly participates in control of expression of other sex control genes of the epinephelus coioides, so that a certain theoretical basis and an important clue are provided for research of sex control of relevant fishes. The encoding protein of the gene Rspo1 is expected to be applied to induced spawning, forced ripening, artificial control sex transformation, large-scale industrial farming of fishes, as well as other aspects.

The invention discloses a bait additive capable of increasing the raised fry survival rate of epinephelus and a use method thereof, and relates to the technical field of aquaculture baits. The bait additive is prepared from natural astaxanthin and rice bran according to the ratio of 1:2, wherein the content of the natural astaxanthin can be 50 mg / kg, 100 mg / kg, 150 mg / kg or 200 mg / kg. The use method of the bait additive comprises a feeding method for epinephelus from incubation to 30 days old, and a feeding method for epinephelus older than 30 days old. According to the invention, the raising survival rate of epinephelus is greatly increased, the cost is reduced, and the astaxanthin is natural in resource and nuisanceless, and has extremely high market popularization value.

The invention relates to an epinephelus coioides interferon IFNgamma2 gene and a protein encoded by the sameas well as application of the protein in preparing a novel immunopotentiator. According to the invention, an open reading frame of the IFNgamma2 gene is cloned from epinephelus coioides head-kidney total RNA by specific primers SEQ ID NO:4 and SEQ ID NO:5 by means of a method of a primer amplification gene segment and RACE overall length amplification. The nucleotide sequence is shown as SEQ ID NO:2. The amino acid sequence of encoded protein is shown as SEQ ID NO:1. An initial test shows that the epinephelus coioides interferon IFNgamma2 protein can induce relevant immunogene expression and can be developed to natural fish immunopotentiator or immunologic adjuvant.

The invention discloses an epinephelus coioides feed additive and an application method thereof. The epinephelus coioides feed additive is composed of a photosynthetic bacteria liquid, a bacillus pumilus powder and a lactobacillus acidophilus liquid. The bacillus pumilus powder is firstly mixed with the lactobacillus acidophilus liquid, and then the mixed bacteria is mixed with the photosynthetic bacteria liquid. The mass percentage content scope of the three compositions comprise that the photosynthetic bacteria liquid accounts for 18-22%, the bacillus pumilus powder accounts for 1-3%, and the lactobacillus acidophilus liquid accounts for 75-81%. Based on 1 Kg of base feed, 10 g-50 g of the additive is added, and the additive is uniformly mixed with the base feed, and then the mixture is wrapped by emulsified fish oil. The epinephelus coioides feed additive is suitable for healthy culture of epinephelus coioides, is capable of obviously improving the activity of SOD, AKP and lysozyme in fish body, thereby effectively promoting growth of epinephelus coioides.

The invention discloses a culturing method for epinephelus coioides. The culturing method comprises steps of 1) preparation before releasing, 2) releasing seedlings, 3) changing food and domestication, and 4) daily management. The beneficial effects are that according to physiological characteristics, a culturing environment condition is controlled; according to growth and development conditions of an Epinephelus coioides digestion system, food is changed slowly and the Epinephelus coioides are domesticated; the Epinephelus coioides have fast growth speed and vigorous vitality; ammonia nitrogen organic matters in water can work as nitrogen source and carbon sources by microorganism agents; the Epinephelus coioides can be biochemically dissolved; beneficial bacteria flora largely propagate to form advanced flora, and poisonous bacteria breeding in water can be prevented; catechol and phenol in water can be removed; flocculation effect can be achieved; and water quality clarity can be improved.

The invention provides a cultivation method for improving disease resistance and stress resistance of epinephelus spp. fries. The cultivation method comprises the following steps of: 1) in two days before fries are added, introducing a variety of skeletonema costatum into a nursery pond; 2) within seven days after fries are added, adding SS type rotifers in each day; 3) during the 8th-15th day offry adding, adding fairy shrimp nauplius and LS type rotifers in each day; 4) during the 16th-25th days of the fry adding, adding the fairy shrimp nauplius in each day; and 5) during the 26th-35th days of the fry adding, adding the fairy shrimp nauplius and compound feed in each day. The nutrients of fingerlings during different periods can be regulated, fry immunity is improved, and the disease resistance and stress resistance of the epinephelus spp. fries can be improved. Through experiments, under a condition that salinity is 3.6%, a survival rate still can be above 68%, a survival rate still can be kept at about 30% under a condition that the salinity is 4.2%, and the survival rate is kept to be 70% or above after the epinephelus spp. fries are infected with vibrio alginnolyficus for two weeks.

The invention discloses a method for selecting nervous necrosis virus resistant individuals of epinephelus lanceolatus. The method comprises the following steps: (1) obtaining SNP site information of the epinephelus lanceolatus; (2) analyzing the disease resistance weight value of the SNP marker and selecting the SNP marker; (3) obtaining candidate epinephelus lanceolatus individual SNP site information and selecting individuals with nervous necrosis virus resistance; and (4) detecting the selection accuracy of disease-resistant individuals. According to the method, the SNP marker with the maximum nervous necrosis virus resisting weight is screened from the SNP markers of the epinephelus lanceolatus, so that a method capable of selecting nervous necrosis virus resisting individuals is established.