13 results about "Epinephelus" patented technology

The invention discloses an artificial cultivation method of a hybrid of epinephelus trifoliatus and epinephelus lanceolatus, which comprises the following steps: taking healthy epinephelus trifoliatus as female parent fish, and carrying out cultivation strengthening and artificial ripening to obtain strengthened female parent fish; healthy epinephelus lanceolatus is used as male parent fish, and sexual mature male parent fish is obtained through natural sexual maturity; the ovulation time and the spermiation time are consistent; collecting egg liquid and seminal fluid, inseminating to obtain fertilized eggs, and performing embryo incubation and illumination regulation and control cultivation after opening to obtain hybrid spots; carrying out authenticity identification on the hybrid spots by utilizing a primer pair, and determining that the hybrid spots inherit genetic information of the female parent fish and the male parent fish; the primer pair is a sequence pair composed of SEQ ID NO. 1 and SEQ ID NO. 2, and a sequence pair composed of SEQ ID NO. 3 and SEQ ID NO. 4. The technical blank of crossbreeding by taking the epinephelus trifoliatus as the parent epinephelus is filled, and the authenticity of the hybridized spots is reliable.

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.

The invention relates to a gene and a plasmid of a humpback perch apoptosis regulator Bad and application of the gene and the plasmid, and belongs to the field of molecular biology, and the sequence of cDNA nucleotide of the humpback perch apoptosis regulator Bad is as shown in SEQ ID NO.1. The invention also provides a construction method of an eukaryotic expression plasmid of the humpback perch apoptosis regulator Bad, and a construction method of the eukaryotic expression plasmid of the humpback perch apoptosis regulator Bad. It is verified that the constructed eukaryotic expression vector has an apoptosis promoting effect on humpback perch cells, and after the humpback perch apoptosis regulatory factor Bad eukaryotic expression plasmid is injected into a fish body, bacterial reproduction can be remarkably inhibited, and a remarkable immune protection effect is achieved.

The present application relates to grouper aquaculture technical field, specifically to a kind of pseudotolithus elongatus wild-like breeding device and method, including frame system and netting system installed on frame system, it is characterized in that, frame system includes platform, the circumferential of platform is equipped with several movable walking frame, walking frame is all connected with the arc-shaped float pipe group, air bag is installed between adjacent float pipe group, air bag is communicated with float barrel, and float barrel is installed at the side of float pipe group far from walking frame;The bionics tube for simulating the wild environment of pseudotolithus elongatus is installed below walking frame;Netting system includes netting and bionic plate installed at the bottom of netting.The present application can improve the survival rate of pseudotolithus elongatus, improve product fish quality, and also can be used for broodstock cultivation.

The invention relates to an artificial cultivation method of low-temperature-resistant Yunjin hybrid spots, and the method belongs to the technical field of aquatic product distant hybridization breeding, and the method comprises the following steps: (1) low-temperature-resistant grouper group breeding and grouper blue-body group breeding; (2) collecting and storing sperms of epinephelus coioides; (3) epinephelus moara reproduction regulation and ovum acquisition; (4) artificial preparation and incubation of hybrid fertilized eggs; compared with the prior art, the fertilization rate of the bred fries is increased by 2.91%, the hatching rate is increased by 9.58%, and the malformation rate is reduced by 11.33%. The survival rate of hybrid seeds is increased by 69.56% compared with that of purebred epinephelus moara, the low-temperature semi-lethal tolerance temperature of new germplasm seeds is 5.8 DEG C, meanwhile, 'Yunjin hybrid spots' have better hybrid growth advantages, and the weight of 7-month-old seeds is increased by 79.72% compared with that of female parent epinephelus moara; compared with that before breeding, the weight gain rate of the hybrid at the same time is increased by 23.20% compared with that of the female parent epinephelus moara.

1. Name of the designed product: sharpener (Epinephelus M0-030). 2. Use of the designed product: a tool for sharpening cutters. 3. Design points of the designed product: in shape. 4. Picture or photo that best shows the design points: perspective view.

This invention discloses a compound feed for juvenile leopard-gill sea bass and its preparation method, belonging to the field of aquatic feed technology. It comprises the following components by weight percentage: 50-70% red fish meal, 3-10% wheat gluten, 10-20% wheat flour, 0.5-1.5% yeast, 1-5% fish oil, 1-5% soybean oil, 0.5-3% soybean lecithin, sea buckthorn flavonoids, and neutral protease, etc. This invention creatively combines neutral protease with sea buckthorn flavonoids, achieving a synergistic effect of promoting digestion and regulating physiological functions. The synergistic effect of these two components promotes rapid growth of the leopard-gill sea bass while effectively enhancing its overall health and stress resistance.

This invention relates to the field of aquaculture technology, and more particularly to a method for large-scale grouper fry rearing based on outdoor-indoor relay rearing. The method includes: using black film ponds as outdoor hatching ponds, disinfecting and conditioning the water before controlling its quality; feeding grouper fertilized eggs in stages after hatching, adjusting the feed according to the mouth size of the fry, and preparing for transfer when 60% of the fry have a mouth size >2200μm; using PVC ponds as indoor rearing ponds, introducing water after multi-stage filtration, disinfection, and detoxification, and controlling the temperature to maintain an indoor-outdoor temperature difference of <2℃; transferring qualified fry to the indoor ponds, disinfecting them with a micro-flow of water, and then distributing them into the rearing ponds; cultivating, strengthening, and disinfecting live feed; feeding the fry in stages indoors according to mouth size, initiating feed acclimatization when 60% of the fry have a mouth size >5mm; controlling the fry density and conducting daily management. The advantages are: integrating the low-cost, large-scale outdoor operation with the high controllability of indoor rearing, improving fry survival rate and seedling quality, and meeting the needs of large-scale aquaculture.

The application provides a low-temperature preservation diluent for sperm of Epinephelus lanceolatus and a use method and application thereof. The diluent is prepared from the following raw materials: glucose, calcium chloride, potassium chloride, sodium chloride, sodium bicarbonate, reduced glutathione, sodium dihydrogen phosphate and fetal bovine serum. The diluent contains the following mass of raw materials per liter: 0.4-0.5 g of glucose, 0.03-0.08 g of calcium chloride, 0.15-0.25 g of potassium chloride, 3-4 g of sodium chloride, 1.5-2.0 g of sodium bicarbonate, 1.5-2.5 g of reduced glutathione, 0.008-0.012 g of sodium dihydrogen phosphate, and 8-12% of the fetal bovine serum in terms of the volume percentage of the diluent. The prepared diluent can better maintain the in-vitro activity of sperm, prolong the sperm preservation time, meet the low-temperature preservation requirements of the sperm of Epinephelus lanceolatus, and especially meet the low-temperature preservation requirements of the sperm of Epinephelus lanceolatus.

The invention relates to the technical field of biological behavioristics, in particular to an epinephelus micropunctatus illumination behavioristics observation experiment device which comprises a center frame, a plurality of interception barriers used for intercepting epinephelus are arranged on the center frame, and the plurality of interception barriers form a cross-shaped frame used for separating the epinephelus; arc-shaped holes are formed in the sides, close to each other, of the bottoms of the intercepting barriers, and the multiple arc-shaped holes form fish passing holes for groupers to pass through; light source assemblies used for emitting light with different colors and brightness are arranged on the side walls of the interception barriers, the interception barriers comprise elastic wrapping layers, and the wrapping layers are fixedly connected with the center frame; and telescopic assemblies for driving the wrapping layers to extend and contract are arranged in the wrapping layers, so that the wrapping layers can adapt to observation environments with different sizes. Through the design of the wrapping layer, the device can adapt to observation environments of different sizes, fishes do not need to be caught and carried, and the efficiency and convenience of an observation experiment are improved.

This invention relates to a method for artificial hybridization breeding of male brown-spotted grouper and female humpback bass. The method belongs to the field of distant hybridization breeding technology. It includes the selection of brown-spotted grouper and humpback bass parents, parent maturation cultivation, gamete collection, artificial insemination and hatching, and seedling cultivation. This invention uses reproductive regulation and distant hybridization technology to hybridize two species belonging to two genera within the grouper family with different geographical distributions and different breeding times, that is, intergeneric hybridization within the grouper family, which breaks through the reproductive isolation between genera, thereby cultivating superior varieties with fast growth and high survival rates.

The application discloses a Epinephelus coioides aquaculture SSR marker, a detection primer thereof and application. The SSR marker comprises ECDSSR-01, ECDSSR-02, ECDSSR-03, ECDSSR-04, ECDSSR-05, ECDSSR-06, ECDSSR-07 and ECDSSR-08. The application is based on the genome data to develop the SSR marker and the primer, the developed SSR marker has higher polymorphism than the marker developed in the prior art, genetic diversity analysis is carried out on 9 individuals, and more than 12 alleles are obtained, which is much higher than the level of 4 to 12 alleles of 20 individuals in the prior art, and has an advantage when evaluating a population with low genetic diversity (such as a proliferation and release population). The developed SSR molecular marker can be applied to the field of genetic diversity analysis of the Epinephelus coioides and the like, and provides a theoretical basis for future investigation of Epinephelus coioides germplasm resources and evaluation of release effect.

The application belongs to the technical field of microsatellite markers, and particularly relates to a kind of multiplex PCR SSR fluorescently labeled primer set of Epinephelus lanceolatus, application and kit and method.The primer set of the application is specific primer pair and universal primer pair;Among them, the specific primer pair is G1 group and / or G2 group, the G1 group is the primer pair El-01-El-05 shown in the base of SEQ ID NO:1-10, and the G2 group is the primer pair El-06-El-10 shown in the base of SEQ ID NO:11-20.The primer of the application is high in polymorphism, stable in product, and accurate and reliable in result;The method is applied in monitoring genetic diversity and germplasm resource protection of Epinephelus lanceolatus.