39 results about "Gill" patented technology

This invention discloses a method for detecting mitochondrial membrane potential in the gills or hepatopancreas of crustaceans, belonging to the field of mitochondrial membrane potential detection technology. This invention, by addressing the structural and physiological differences between gill and hepatopancreas tissues in crustaceans, formulates differentiated incubation strategies: for gill tissue, a "live pre-equilibration-short-time staining-rapid slide preparation" method is used, strictly controlling the JC-1 incubation time to within 10 minutes to effectively avoid probe toxicity damage to mitochondria; for hepatopancreas tissue, in vivo incubation is extended to 1 hour to ensure sufficient dye penetration. Under the condition of uniformly using 2 μM JC-1 and 20 μmol / L CCCP as positive controls, this method significantly improves the tissue penetration and signal uniformity of the dye, solving problems such as uneven staining and weak signal caused by exoskeleton barriers and high lipid content, providing reliable technical support for aquatic health assessment and toxicology research.

The invention discloses a method for marking strontium in enhanced and released crustacean yoke cardia bones, and relates to the field of fishery resources and environments. The method comprises the following steps: culturing the crustacean aquatic animals in a strontium-containing water body, and labeling the yoke cardia bones of the crustacean aquatic animals with strontium. According to the method, various potential target tissues of crustaceans are systematically screened, it is determined that the yoke cardia bone is the most suitable target organ marked by the strontium element for the first time, the enrichment effect of the strontium element is remarkable and stable, the marking characteristics are clear and easy to detect, and the problem that due to shell molting, a traditional marker is prone to falling off is effectively solved. Compared with a conventional method, the marking cost is greatly reduced, and the economical advantage is outstanding; and meanwhile, by virtue of the strong specificity of the element fingerprints, a release group and a natural group can be accurately distinguished, the core requirements of enhancement release on the stability and traceability of a marking technology can be met, and an efficient and reliable technical support is provided for release effect evaluation and population tracking management in crustacean wild resource restoration.

The invention relates to a fish respiratory rate detection method based on YOLO segmentation and optical flow tracking, and belongs to the technical field of aquatic organism physiological status monitoring. The method comprises the steps of video data input and initialization, video preprocessing and frame rate dynamic calibration, gill segmentation by a YOLO model and target screening, gill region-of-interest extraction, optical flow feature point tracking and dynamic maintenance, gill motion displacement calculation and signal optimization, respiratory rate calculation and smooth optimization, and result output and visualization. Under complex scenes such as water wave interference and fish swimming, the respiratory rate calculation error is smaller than or equal to + / -2 BPM, the effective data rate is larger than or equal to 95%, and the method is superior to the prior art (for example, the patent CN120599516A) and can be applied to scenes such as aquaculture health early warning and aquatic organism physiological experiments.

The present application relates to the field of aquaculture technology, and particularly relates to a culture method of bigmouth bass gill explants and application. The present application provides a culture method of bigmouth bass gill explants, which uses explant culture solution to culture the gill explants of bigmouth bass. The explant culture solution is formed by adding fetal bovine serum, fish serum, trinity, D-galactose, L-alanyl-L-glutamine, sodium pyruvate and melatonin into a basic culture medium. In combination with dynamic culture conditions, the natural three-dimensional structure and intercellular connection of the gill tissue can be preserved, and the metabolic activity and functional integrity thereof can be maintained. The present application can solve the technical problem that the prior art cannot provide an ideal research model which can both maintain the structural integrity of the gill tissue and accurately reflect the functional characteristics of the gill tissue. Through the culture method, research models such as ammonia nitrogen stress can be efficiently constructed, thereby providing technical support for the research of bigmouth bass gill tissue physiological mechanism, disease prevention and control, environmental risk assessment and the like.

A device collects aquatic field metabolic rate data related to a water-breathing animal having at least one gill opening. The device has at least one oxygen probe, which measures and records oxygen consumption or oxygen extraction data at the at least one gill opening. The device is configured to collect metabolic data when the animal is in a natural environment. A method for determining a metabolic rate of the animal located in a water environment includes measuring metabolic rate data via at least one gill oxygen probe which is inserted into the at least one gill opening; measuring ambient data in the water environment via at least one ambient oxygen probe; and estimating a whole animal metabolic rate.

The invention relates to a river crab bean curd premade dish based on whole-course non-thermal processing and a making method thereof, and belongs to the technical field of aquatic product processing. A preparation method of a river crab bean curd premade dish based on whole-course non-thermal processing comprises the following steps: removing crab shells, gills, mouth umbilicus, heart and stomach and intestine of river crabs, crushing and filtering to obtain crab meat juice; and then adding the yeast extract and Arabic gum, stirring and mixing, homogenizing at high pressure, carrying out vacuum plastic packaging, sterilizing at ultrahigh pressure, and freezing to obtain the product. According to the method provided by the invention, through combination and synergy of a series of non-thermal processing technologies, the original flavor of the river crabs is reserved and improved to the greatest extent on the premise of ensuring the microbial safety of the product, and meanwhile, the characters of the final product are optimized, so that industrial production and long-term storage can be realized, and excellent quality can be kept.

This invention belongs to the field of bio-aquaculture technology, specifically relating to SNP loci related to the body color of the leopard-gill spiny perch and their applications. This invention provides SNP loci (16_797851, 13_24322516, and 9_17302939) associated with the body color of the leopard-gill spiny perch. Furthermore, this invention provides a method for mining SNP loci and a method for enhancing the red color of the leopard-gill spiny perch's skin. By employing the HSV color model, the red body color is transformed from a direct sensory representation into a continuous quantitative variable, providing a data foundation for the genetic analysis of body color traits. Based on genome-wide molecular marker screening, genetic markers significantly associated with body color traits are obtained. Combined with the administration of agonists to individuals with genetic advantages, a significant enhancement of the red color of the leopard-gill spiny perch's body surface can be achieved. Therefore, the technical solution of this invention can not only directionally improve the body color trait of the leopard-gill spiny perch and cultivate high-quality new strains with stable body color inheritance, but also promote the development of marine aquaculture and the seed industry.

The utility model belongs to the technical field of shrimp gill tissue crushing, and particularly relates to a quick crusher for gill tissues of penaeus vannamei boone, which comprises an adjusting base, an adjusting type container cooling mechanism is mounted at the top of the adjusting base, and the adjusting type container cooling mechanism comprises a plurality of cooling clamping plates. The multiple cooling clamping plates are annularly arranged and slidably mounted above the adjusting base, and circulating cooling channels are formed in the cooling clamping plates. A cooling liquid storage box is arranged on one side of the adjusting base, an electric lifting push rod is fixed to the top of the cooling liquid storage box, a supporting plate is fixed to the top of the electric lifting push rod, and a first motor is fixed to the top of one end of the supporting plate. According to the utility model, through the cooling system which can be moved and adjusted, by adjusting the position of the cooling system, the containers with different specifications can be attached and fixed, the low temperature in the containers can be maintained, the containers are convenient to disassemble and replace, the containers with proper capacities can be selected according to required sample sizes, and the use is more convenient.

This invention provides an integrated automatic processing device for crayfish, comprising: a frame, an inverted slide assembly, a Z-shaped cantilever, an adaptive clamping mechanism, a deveining mechanism, a head removal mechanism, and a back-opening and gill-removing mechanism. The inverted slide assembly is mounted on the frame. The lower end of the Z-shaped cantilever is fixedly connected to a slider. The adaptive clamping mechanism is mounted on the Z-shaped cantilever to fix the crayfish body. The deveining mechanism is coaxially arranged with the adaptive clamping mechanism. The head removal mechanism and the back-opening and gill-removing mechanism are sequentially arranged above the moving path. This invention achieves physical isolation between the transmission area and the processing area through the inverted slide and the Z-shaped cantilever, avoiding contamination and jamming. It employs a single-servo linkage type double-claw adaptive clamping, reducing the power source. Deveining is achieved through clamping and pulling; head removal uses an electric push rod-driven arc-shaped rotary cutter in conjunction with the slide's backward shearing; and back-opening and gill-removing uses a single motor-driven circular saw blade and a double-reverse steel wire brush, achieving integrated automatic processing of deveining, head removal, back-opening, and gill removal.

The invention discloses a steppe red oomycete strain, a microbial agent thereof and application of the steppe red oomycete strain in aquaculture, the steppe red oomycete strain is sent to China General Microbiological Culture Collection Center (CGMCC) for preservation, the preservation number is CGMCC No.35769, and the preservation date is August 28, 2025. The strain disclosed by the invention is subjected to anaerobic illumination culture in a conventional photosynthetic bacterium liquid culture medium to form a red viscous trait microbial agent, and the degradation rate of the microbial agent on H2S in water can reach 90% or above; meanwhile, the water purification effect is achieved when the fertilizer is applied to a culture pond with organic matter exceeding the standard, odor can be eliminated, and water can be cleared. The strain disclosed by the invention can effectively block toxic stress such as gill injury, hepatopancreas poisoning and the like of H2S to fish, shrimp and crab culture organisms and remarkably improve culture water environment and bottom mud quality, so that an efficient and environment-friendly novel microbial resource is provided for water H2S pollution treatment in aquaculture; the method is especially suitable for high-density pond culture and has wide practical application prospects and popularization value.

The invention discloses a multi-stage purification recirculating aquaculture system for plectropomus leopardus, and relates to the field of fish culture equipment. Comprising the steps that a culture pond body is designed in a partitioned mode to be matched with plectropomus leopardus to inhabit and swim, multi-stage purification treatment modules are arranged in sequence to guarantee clean water quality, a water quality real-time monitoring module collects parameters in the whole area and synchronizes the parameters to a control center, and an intelligent water circulation driving module achieves efficient water circulation through a variable frequency water pump and a pipeline. The dynamic regulation and control module adjusts breeding environment parameters based on monitoring data, the precise feeding module sets a feeding scheme according to feeding habits, the pollution discharge collection and treatment module achieves waste recycling, and the system monitoring and early warning module stores data in a classified mode and conducts early warning in a classified mode. Through multi-stage purification and intelligent regulation and control, the aquaculture water quality is stabilized, diseases are reduced, and the aquaculture survival rate and the product quality are improved; pollution is reduced through precise feeding and pollution discharge design, cost is saved through an energy consumption optimization module, waste recycling meets the environmental protection requirement, and large-scale efficient breeding is assisted.

This invention discloses a method for constructing and applying a marine fish muscle stem cell line. The culture medium uses basal medium as the background medium and contains LIF growth factor, EGF growth factor, and FGF2 protein derived from the leopard-gill sea bass. The final concentration of FGF2 protein from the leopard-gill sea bass is 20-30 ng / mL, 30-45 ng / mL, 45-55 ng / mL, or 55-70 ng / mL. Passaging using this medium can construct a stable, rapidly dividing, and short-passaging immortalized marine fish muscle stem cell line, capable of stable passage for more than 50 generations. This provides raw materials for exploring the function and gene regulation mechanisms of myoblasts, and provides in vitro verification experimental conditions for further research on the effects of marine fish muscle stem cells on growth rate, laying the foundation for the breeding of superior marine fish species.

The invention relates to the technical field of animal morphological research and specimen making, in particular to a microminiature fish skeleton specimen making method which comprises the following steps: step 1, standardized trapping and feeding of white skin beetles: preparing a feeding box and a worm-eaten box, trapping and feeding the white skin beetles, and screening out vigor larvae with the body length of more than or equal to 0.5 cm for later use; step 2, pretreatment of fish materials: selecting microminiature sclerobone fish with the body length of less than 10cm, freezing to death, sterilizing and dehydrating with 75% or more alcohol, and drying and shaping at the temperature of less than 65 DEG C after primary treatment of epidermis; according to the method, accurate fleshing is conducted on the larvae of the white skin beetles, damage to fine skeletons such as fin rays and gill bones is close to zero, the problem of structural damage of a traditional fleshing method is solved, the fine skeleton features of a specimen are clear and distinguishable, a standardized breeding system and an atomization bleaching process of the white skin beetles are established, the risk of skeleton disintegration of traditional soaking bleaching is avoided, and the method is suitable for large-scale popularization and application. And the manufacturing process is repeatable and small in error.

The invention discloses a self-adaptive freshwater fish scaling and sectioning all-in-one machine, and belongs to the technical field of aquatic product processing mechanical equipment. The all-in-one machine is sequentially provided with a scaling module, a conveyor belt self-adaptive centering conveying module, a gill removing module, a laparotomy module and a viscera removing module along a processing assembly line. The device is characterized in that the conveyor belt module adopts a symmetrical rocker arm connecting rod mechanism to realize self-adaptive adjustment of the clamping width; the belly cutting module is provided with a follow-up lifting mechanism and is matched with real-time fish belly contour detection to realize accurate cutting; the viscera removing module adopts a profiling brush wheel to clean the abdominal cavity; and the gill removing module realizes automatic gill removing. The equipment is high in integration level and strong in adaptability, and integrated automatic processing from scaling to viscera removing of various freshwater fishes is realized.

The utility model relates to the technical field of crayfish processing equipment, and discloses a bubble cleaning machine for crayfish processing, the bubble cleaning machine comprises a cleaning box body, a bubble generating device, a filtering device and a circulating water pump, the cleaning box body is of a cuboid structure, the top of the cleaning box body is open, and a water inlet pipe is arranged at the upper part of one side of the cleaning box body; the bubble generating device comprises an air pump, a main air pipe, branch air pipes and bubble stones, the multiple branch air pipes are arranged on the outer walls of the two sides of the main air pipe and communicate with the main air pipe, the multiple bubble stones are arranged on the outer walls of the tops of the multiple branch air pipes, the air pump communicates with the main air pipe through an air conveying pipe, and the main air pipe communicates with the main air pipe; according to the crayfish cleaning device, a large number of tiny bubbles are generated through the bubble generating device, so that water is strongly turned over and stirred, crayfishes can be cleaned more efficiently and thoroughly, particularly impurities hidden in the parts such as gills and feet of the crayfishes can be effectively removed, and the cleaning effect is good.

The present application belongs to the field of crayfish processing, and particularly relates to a crayfish processing positioning device, which comprises a supporting table, a cleaning box fixed to the top of the supporting table, a plurality of ultrasonic vibration plates fixed to the inner walls of the cleaning box, an ozone generator arranged on one side of the supporting table, an exhaust duct fixed to the bottom of the inner cavity of the supporting table, a movable positioning mechanism acting on the crayfish body, and one side of the exhaust duct penetrating through the supporting table and being fixedly arranged in communication with the output end of the ozone generator. The positioning box is driven by the driving assembly to move reciprocally in the horizontal direction, so that the crayfish body placed in the inner cavity of the positioning box can be subjected to the scouring action of the water flow, thereby improving the cleaning effect of the crayfish body, preventing the insufficient removal rate of the parasites and pathogenic bacteria in the narrow gaps such as the gill folds and the abdominal joint, and improving the uniformity of the anaerobic contact of the crayfish body in the inner cavity of the positioning box.

The invention relates to the technical field of deep sea aquaculture, in particular to an air supplementing and feeding method and system suitable for swimming bladder fishes. A pneumatic type bait casting device is used for conducting air supplementing and feeding on swimming bladder fishes in a deep-sea breeding box, the pneumatic type bait casting device comprises a high-pressure fan, a venturi pipeline and a bait conveying pipe which are sequentially connected, and the venturi pipeline is further communicated with a bait storage bin. The high-pressure fan is communicated with the bait storage bin through the pressure compensation pipe to balance the pressure difference between the bait storage bin and the Venturi pipeline, so that the bait can enter the Venturi pipeline from the bait storage bin; after the high-pressure fan is started and lasts for a set time, bait is conveyed into the Venturi pipeline according to the feed-gas ratio of (12-20): 1, high-pressure gas is used as a conveying medium, and both dry feed and live feed can be used for feeding; an air supplementing device does not need to be additionally arranged, the equipment cost is reduced, and the air supplementing and feeding requirements of the laryngeal fishes such as salmons in the large deep-sea aquaculture box can be met.

The invention discloses a Crassostrea sikamea gill tissue color phenotype identification method, which comprises: extraction: stripping a gill tissue of Crassostrea sikamea, carrying out homogenization and series solvent treatment, carrying out extraction by using an ammonia water solution, carrying out centrifugation treatment, collecting a supernatant, and filtering to obtain a color generation substance extraction solution; detection: determining the absorbance of the color generation substance extracting solution under a specific wavelength based on a spectrophotometric method; and identification: distinguishing the color phenotypes of the gill tissues according to the absorbance values. The method is easy to operate, reliable in result and good in repeatability, traditional artificial visual judgment depending on experience is converted into objective measurement based on spectral data, the misjudgment risk caused by human subjective factors is thoroughly eliminated, and standardized and quantifiable identification of the gill tissue color phenotype is achieved. Besides, the method is based on a spectrophotometric method, interference of reflected light caused by surface textures, wrinkles and mucus of the gill tissue in measurement of a traditional colorimeter is avoided, and therefore a stable and accurate quantitative result is obtained.

The utility model belongs to the field of cooling equipment, and particularly relates to a fin structure imitating shark gill fissure. The fin structure comprises bionic curved surface fins and flow channel partition plates. A plurality of bionic curved-surface fin sets are arranged between the two flow channel partition plates in an array mode, each bionic curved-surface fin set comprises two bionic curved-surface fins which are symmetrically arranged, the two bionic curved-surface fins of the same bionic curved-surface fin set are oppositely arranged, and a first flow channel is formed between the two bionic curved-surface fins. The two bionic adjacent curved-surface fins of the adjacent bionic curved-surface fin groups are arranged oppositely, a second flow channel is formed between the two bionic adjacent curved-surface fins, and the cross sectional areas of the first flow channel and the second flow channel are changed periodically; the bionic curved-surface fin is formed by a plurality of shark-imitating-appearance curved-surface fin single bodies in an array mode, a plurality of shark-imitating-appearance curved-surface fin openings are formed in the middles of the shark-imitating-appearance curved-surface fin single bodies, and an inclined gill split piece is arranged on the edge of each shark-imitating-appearance curved-surface fin single body. The synergistic effect of a temperature field and a velocity field is enhanced while the heat exchange area is increased, and the heat exchange performance of the heat exchanger is improved.

The invention belongs to the technical field of biological culture, and particularly relates to an SNP site related to the body color of plectropomus leopardus and application of the SNP site. The invention provides SNP (Single Nucleotide Polymorphism) sites (16797851, 1324322516 and 917302939) related to the body color of the plectropomus leopardus, and the SNP sites are related to the body color of the plectropomus leopardus. In addition, the invention further provides a digging method of the SNP sites and a method for improving the red color of the skin of the plectropomus leopardus. By adopting an HSV color mode, a red body surface color is converted from visual sensory representation to continuous quantitative variables, and a data basis is provided for genetic analysis of body color traits; based on whole genome level molecular marker screening, a genetic marker significantly associated with the body color character is obtained, and a gene dominant individual is fed with an agonist, so that the body surface red color of the plectropomus leopardus can be significantly improved. Therefore, according to the technical scheme, the body color character of the plectropomus leopardus can be directionally improved, a high-quality new strain with stable inheritance of the body color can be cultivated, and the development of the mariculture industry and the breeding industry can be promoted.

This invention discloses a compound feed for juvenile leopard-gill sea bass and its preparation method, belonging to the field of aquatic feed technology. It comprises the following components by weight percentage: 50-70% red fish meal, 3-10% wheat gluten, 10-20% wheat flour, 0.5-1.5% yeast, 1-5% fish oil, 1-5% soybean oil, 0.5-3% soybean lecithin, sea buckthorn flavonoids, and neutral protease, etc. This invention creatively combines neutral protease with sea buckthorn flavonoids, achieving a synergistic effect of promoting digestion and regulating physiological functions. The synergistic effect of these two components promotes rapid growth of the leopard-gill sea bass while effectively enhancing its overall health and stress resistance.

The application discloses a bacillus megaterium strain for increasing adversity tolerance of aquatic animals and application thereof. The bacillus megaterium strain is named bacillus megaterium SCAU-Ohb1, the strain has been preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO.62119, and the preservation date is December 13, 2021. The bacillus megaterium SCAU-Ohb1 is isolated from fish intestinal tracts and is safe to fish bodies. After the fermentation liquor of the bacillus megaterium SCAU-Ohb1 is mixed with a basic daily ration and is used for feeding freshwater fish, the activity of important antioxidant enzymes in the fish bodies can be significantly improved, the salt tolerance of gill tissues of the freshwater fish under acute salt stress can be increased, and the survival rate of the fish bodies can be improved. The application provides a new method for improving adversity tolerance of aquatic animals, has important significance for the aquaculture industry, and is expected to improve production efficiency and reduce losses.

The application provides a method for nondestructive detection of the contents of EPA and DHA in scallop adductor muscle based on Raman spectrum, which comprises the following steps: firstly, a proper amount of gill filament tissue is taken from a living scallop by a nondestructive method, and then a frozen section is prepared; secondly, the section is subjected to Raman laser scanning to obtain original data; and finally, after pretreatment such as normalization, noise reduction and baseline correction, a characteristic wavelength is selected, and a model for predicting the content of unsaturated fatty acid in the adductor muscle by using the gill filament Raman data across tissues is established in combination with the lipid group data of the adductor muscle. Compared with the existing method for detecting the content of unsaturated fatty acid in scallops, the determination method of the application can realize the rapid, accurate and simple detection of the content of unsaturated fatty acid in the main edible part, i.e., the striated muscle of scallops, under the condition of ensuring the survival of scallops, and greatly reduces the detection cost, thereby providing an efficient and simple technical means for the breeding of shellfish quality traits, and being beneficial to the popularization and promotion in the comprehensive evaluation and genetic improvement of aquatic germplasm.

The invention discloses a method for detecting mitochondrial membrane potential of gill or hepatopancreas of crustaceans, and belongs to the technical field of mitochondrial membrane potential detection. Differentiated incubation strategies are respectively formulated according to the structure and physiological difference of gill and hepatopancreas tissues of crustaceans: a method of'in-vivo pre-balancing-short-time staining-rapid flaking 'is adopted for the gill tissues, JC-1 incubation time is strictly controlled within 10 min, and damage of probe toxicity to mitochondria is effectively avoided; hepatopancreas tissues are incubated for 1h by adopting a prolonged living body, so that the sufficient permeation of the dye is ensured. According to the method, under the condition that 2 [mu] M JC-1 and 20 [mu] mol / L CCCP are uniformly used as positive control, the tissue permeability and signal uniformity of the dye are remarkably improved, the problems of uneven dyeing, weak signals and the like caused by exoskeleton barrier and high lipid content are solved, and reliable technical support is provided for aquatic product health assessment and toxicological research.

The application provides a fish individual and group stress nondestructive in-situ detection system and method, and relates to the technical field of stress evaluation.The system comprises: a multi-modal sensing module using a flexible sensing chip and an impedance hydrogel electrode to collect real-time breathing, environmental and physiological impedance data of the gill and back of a fish body; a rear-end circuit module to complete data collection, processing and wireless transmission; a stress data determination module to perform feature extraction and nonlinear fusion on the screened data based on a multi-modal data fusion algorithm, to quantify fish stress levels and calculate stress fatigue, and to trigger an early warning and generate an environmental control instruction when a high stress threshold is reached.The application realizes non-invasive, high-precision real-time monitoring and closed-loop feedback control of the stress state of fish during transportation, and significantly improves the survival rate of fish transportation.

The invention relates to a channel catfish gill cover edge detection mechanism and method. The channel catfish gill cover edge detection mechanism comprises a fish body clamp; the probe assembly is provided with a lead screw module controlled by the electric control system, and the probe assembly is driven by the lead screw module to integrally move, make contact with a fish body and detect the fish body; wherein the probe assembly comprises a plurality of probes and a spring box base, each probe is provided with a pinhole sliding block, a probe spring and a pressure sensor, the pinhole sliding block is in sliding fit with the spring box base in the detection direction, the pressure sensor is fixedly connected with the spring box base, and the front end and the rear end of the probe spring are connected with the pinhole sliding block and the pressure sensor respectively. According to the probe assembly provided by the invention, the probes are connected with the springs through the special pinhole sliding blocks, and the identification and positioning of the gill cover edge of the channel catfish can be completed under the conditions that the detection width is 3-5mm and the detection precision is 1-1.5 mm through the adjustment and combination of the number, the diameter and the distance of the probes, so that the problem of bloodletting point positioning of bloodletting equipment is solved.

The utility model relates to the technical field of side slope drainage, and discloses a high and steep side slope greening solidified fiber soil drainage dredging structure which comprises a high and steep side slope body composed of greening solidified fiber soil, a plurality of vertical drainage grooves are poured in the slope face of the high and steep side slope body, and transverse water guide grooves are poured between every two adjacent vertical drainage grooves. A plurality of drainage pipes are embedded in the high and steep slope main body, and water outlets of the drainage pipes are formed in the vertical drainage grooves or the transverse water guide grooves; the water outlet end of the drainage pipe is in threaded connection with a protective cap, a water outlet hole is formed in the front end of the protective cap, and a gill net is arranged at the water outlet hole and fixedly installed on the protective cap. The protective cap is in threaded connection with the drainage end of the drainage pipe, drainage is facilitated through the water outlet hole, birds or small animals are prevented from entering the drainage pipe through the gill net at the water outlet hole, and therefore the animals are prevented from inhabiting or nesting in the drainage pipe, and normal use of the drainage pipe is avoided.

The invention belongs to the technical field of body color stabilization of freshwater fishes in cold-chain transportation and storage. The invention relates to a body color protection method in cold-chain transportation and storage of pelteobagrus fulvidraco based on a color protection preservative. The body color protection method is characterized by comprising the following steps: 1) preparing a color protection preservative solution; 2) preparing a body surface protective film solution; (3) temporarily rearing fresh pelteobagrus fulvidraco for 6 hours to relieve transportation stress, stirring in an ice-water bath, slaughtering, removing viscera and gills, cleaning, and simply draining; soaking the drained pelteobagrus fulvidraco in the color-protecting preservative solution obtained in the step (1) for 3 hours; then uniformly spraying the body surface protective film solution prepared in the step 2) on the surface of the fish body; (4) putting the pelteobagrus fulvidraco subjected to vacuum packaging in the step (3) into a quick-freezing warehouse for quick-freezing; (5) in the transportation and storage stages, proper environmental conditions are kept; and 6) taking out the quick-frozen pelteobagrus fulvidraco from the vacuum bag, and directly thawing the pelteobagrus fulvidraco together with the protective film on the surface in a running water thawing manner. The method is remarkable and stable in color protection effect.

The application discloses a desert Caenispirillum deserti CDCHPC1 from Qinhao Island and application of the Caenispirillum deserti CDCHPC1 in purifying inorganic nitrogen and phosphorus in water bodies, and belongs to the technical field of purifying seawater aquaculture water quality by microorganisms. The Caenispirillum deserti CDCHPC1 has been preserved in the Guangdong Provincial Microbial Culture Collection Center, and the preservation number is GDMCC No: 64120, and the preservation time is December 6, 2023. The strain CDCHPC1 disclosed by the application is screened from the gill and digestive tract tissue of Pristipomoides sieboldii in the Qinhao Island sea area, has no adverse effect on farmed fish and shrimp, has strong purifying capacity on nitrite, nitrate and phosphate in the aquaculture water body, and has good environmental adaptability. The application solves the practical demand of purifying the water environment of high-density seawater fish and shrimp culture, and has a wide application prospect in the field of purifying water bodies.

The invention discloses establishment and application of gill cells of gobiocypris rarus, gill of gobiocypris rarus is utilized to establish gill cells Gbr-gill of gobiocypris rarus, and the gill cells Gbr-gill are gill cell lines of gobiocypris rarus which can be continuously passed. The culture method of the gobiocypris rarus gill cell line comprises the steps of primary culture, subculture and cryopreservation and resuscitation of the gobiocypris rarus gill cell line. A chemical and environmental water acute toxicity assessment method is established based on the gobiocypris rarus gill cell line, and the method specifically comprises the following steps: inoculating gobiocypris rarus gill cells, exposing 3, 4-dichloroaniline and environmental water, and detecting the toxic effect of 3, 4-dichloroaniline on the gobiocypris rarus gill cells. According to the establishment method and application of gill cells of gobiocypris rarus, the application of gobiocypris rarus in toxicity detection of chemicals and environmental water is expanded, and reference is provided for acute toxicity test based on fish cells.