77 results about "Gill" patented technology

The invention relates to the technical field of cell separation and culture, in particular to a method for separating and culturing haematochrome cells of plectropomus leopardus. The separation method of the plectropomus leopardus haematochrome cells is created for the first time, the EDTA digestive juice is used for pre-digesting epidermis cells on the outer layers of the plectropomus leopardus fins, then the collagenase digestive juice is used for digesting connective tissues (pigmented cells) of internal dermis, digestive enzymes such as trypsin and other mechanical methods which have great damage to the cells are not used, and the method is suitable for large-scale production of the plectropomus leopardus haematochrome cells. The plectropomus leopardus haematochrome cell can reduce cell damage, can be effectively separated to obtain the plectropomus leopardus haematochrome cell which is high in purity, good in activity and capable of being cultured in vitro, and lays a foundation for subsequent experiments such as single cell sequencing, cell in-vitro culture and molecular function verification.

The invention provides a breeding method of hypoxia-resistant golden tiger hybrid spots, which comprises the following steps: carrying out artificial insemination on a female parent of epinephelus coioides and a male parent of epinephelus coioides to obtain fertilized eggs, carrying out 2.8-3.2 mg / L hypoxia screening on the female parent for more than three generations, and ensuring that the suffocation point is less than or equal to 0.9 mg / L. Performing staged low-oxygen incubation on the fertilized eggs, gradually reducing dissolved oxygen in an embryonic period, a protointestinal period and a membrane emergence period, performing specific sound wave stimulation in an opening period of larva fish, performing low-oxygen-resistant phenotype screening in a larva period, performing intermittent low-oxygen intensive training in a juvenile fish period, applying water flow stimulation before training, and performing low-oxygen-resistant phenotype screening in a membrane emergence period; during the period, a fortified feed containing gamma-aminobutyric acid, heme iron and other components is fed. According to the method, through multi-link collaborative innovation, the hypoxia resistance of the Jinhu hybrid spots is effectively improved, the survival rate of a target group under 2.5 mg / L dissolved oxygen reaches 93%, the suffocation point is reduced to 0.75 mg / L, meanwhile, gill filament development and growth are promoted, and an efficient technical scheme is provided for aquatic product breeding.

The invention provides an automatic bait casting system for training and breeding juvenile plectropomus leopardus, which comprises a basic data module connected with an automatic bait training device and used for acquiring original data of a breeding pond and extracting feeding basic data; the dynamic association module is used for carrying out multi-level cross association modeling, causal inference association, sub-mode mining and classification adaptation to obtain an ingestion-turbidity dynamic association model and outputting an ingestion-turbidity relationship; the hunger index module is used for constructing a hunger index model and outputting the hunger index of each culture pond; the feeding strategy module is used for calculating the next feeding interval, the strip-shaped bait-live bait proportion, the actual time point of next feeding and the bait amount per hour and judging whether extra meal or increase of the next feeding interval is needed or not to obtain a self-adaptive feeding strategy; and the feeding driving module is used for controlling the running action of the automatic bait training device. Scientific feeding can be achieved, the feeding survival rate, the growth rate and the bait training effect are remarkably improved, feed waste is reduced, and intelligent and automatic management of the whole process is achieved.

The invention discloses a marine crab radiation dose estimation method based on a voxel model, which obtains detailed anatomical data of marine crabs by using a CT scanning technology, constructs an accurate voxel model, and can effectively distinguish organs such as crab shells, crab meat, hearts, gills and crab spawns. The process avoids the defect that a traditional simplified model neglects organ differences, so that the accuracy of radiation dose estimation is improved. According to the method, the radiation absorption characteristic of each organ can be accurately simulated, and a more real radiation dose calculation result is provided. The three-dimensional anatomical data of the living body can be accurately obtained based on the CT and image processing technology, so that the technical scheme has high adaptability, is suitable for marine crabs, can be flexibly adjusted according to anatomical features of different species, and can be widely applied to the fields of marine crabs, marine crabs and marine crabs. The method is widely applied to radiation dose estimation of other aquatic organisms or land species.

The invention discloses an indoor detoxification seedling raising method for plectropomus leopardus, and relates to the technical field of biology. The seedling raising method comprises the steps that after an indoor seedling raising pond is sterilized and killed, newly hatched seedlings of plectropomus leopardus are put into the indoor seedling raising pond, and after the newly hatched seedlings open to eat food, different sterilized and killed baits are adopted for feeding the seedlings in a stepped mode for seedling raising culture. According to the method, multiple baits such as nannochloropsis oculata, oyster larvae, SS rotifers, S rotifers, L rotifers, fairy shrimp nauplius larvae, copepod nauplius larvae, copepod middle worms, fairy shrimp adults and copepod adults are fed in a stepped mode, rapid growth of the plectropomus leopardus juvenile fish is promoted, the disease resistance of the juvenile fish is effectively improved, the environment adaptability is enhanced, and the survival rate of the plectropomus leopardus is increased. And the survival rate is well improved.

The invention provides a full-indoor artificial breeding method for plectropomus leopardus, and belongs to the technical field of artificial breeding of marine fishes. The full-indoor artificial breeding method for the plectropomus leopardus comprises the steps of earlier-stage incubation of fertilized eggs, incubation in an indoor breeding pond, breeding in an indoor flowing water pond, breeding in an indoor circulating water pond and the like. By regulating and controlling illumination, the irritability of the fries is greatly reduced, the ecological conditions of the fries are effectively optimized, and the health condition and adaptability of the fries are improved. Meanwhile, different types and different total amounts of biological bait combinations are fed in the larva stage, the juvenile stage and the fish stage of the plectropomus leopardus, transition is made in each bait transfer period, enteritis caused by excessive intake of larvae and juveniles and too little intake caused by inadaptation of bait transfer are effectively reduced, and the problem that the survival rate is low in the bait transfer period can be effectively solved.

The invention discloses a method for reducing environmental stress of waterless keep-alive shellfish, and belongs to the technical field of keep-alive of aquatic products. The method comprises the following specific steps: cleaning caught fresh and live shellfishes, and temporarily culturing the shellfishes in a seawater temporary culture pond containing a shellfish stress inhibitor for 2-24 hours; performing cooling treatment on the temporarily cultured shellfish; draining the cooled shellfish, putting the shellfish into a packaging bag of which the inner wall is attached with a bionic gill filament film, and carrying out water-free keep-alive transportation or storage in a refrigeration environment of 2-4 DEG C; a shellfish stress inhibitor is used in the temporary rearing pond at the transportation or storage end point, and constant-speed heating is kept. Through the synergistic effect of the shellfish stress inhibitor and the bionic gill filament membrane, a more stable and suitable waterless keep-alive environment is provided for shellfishes, in the waterless keep-alive process of the shellfishes, the cell damage and death rate are reduced, and the survival rate is remarkably improved; meanwhile, glycogen consumption is reduced, the mass loss rate and the oxidative stress level of the shellfish are reduced, the survival rate of the shellfish is increased, the good texture of the shellfish is kept, and the method has important value for integrally improving the economic benefit of the waterless keep-alive shellfish.

The invention discloses a low-stress bleeding processing method for channel catfish. The method comprises the following steps: an acid-base control step, in which the channel catfish is placed in water containing sodium citrate to control the acid-base in the body, thereby increasing the alkali reserve in the body of the fish; a combined light anesthesia step, in which the channel catfish is lightly anesthetized through the combined action of low temperature and a chemical anesthetic, so that the fish exhibits a state in which the gill cover of the fish opens and closes weakly and slowly, and the fish body rolls over and loses balance; a fish body puncture step, in which the blood circulation of the channel catfish is destroyed by a manual or mechanical method, thereby creating a bleeding wound on the fish body; a metabolic bleeding step, in which the fish body is placed in a bleeding tank that has been aerated and oxygenated in advance to saturated dissolved oxygen, for bleeding, while maintaining a certain dissolved oxygen concentration; and an ultrasonic blood purification step, in which the fish body is placed in a bleeding tank equipped with an ultrasonic device for bleeding, thereby accelerating the discharge of residual blood in the body.

The invention discloses a channel catfish gill bloodletting point self-adaptive positioning mechanism which comprises a horizontal sliding block seat in transmission connection with a driving component and driven by the driving component to move front and back. The tool apron is connected with the horizontal sliding block base in a left-right sliding mode and can conduct self-adaptive movable adjustment relative to the horizontal sliding block base, the sliding direction of the tool apron is perpendicular to the sliding direction of the horizontal sliding block base, spring bases are fixedly connected to the left side and the right side of the horizontal sliding block base correspondingly, and spring sets are connected to the left side and the right side of the tool apron correspondingly. The left side / right side of the tool apron is elastically connected with the spring seat on the left side / right side through a spring group; the cutter module is used for puncturing a fish body and can be adjusted in a self-adaptive mode, and the cutter module is rotatably, movably and adjustably connected with the cutter holder. By adopting the scheme of the invention, the problems of gill bloodletting point identification and positioning in the prior art can be solved.

This invention discloses a method for detecting mitochondrial membrane potential in the gills or hepatopancreas of crustaceans, belonging to the field of mitochondrial membrane potential detection technology. This invention, by addressing the structural and physiological differences between gill and hepatopancreas tissues in crustaceans, formulates differentiated incubation strategies: for gill tissue, a "live pre-equilibration-short-time staining-rapid slide preparation" method is used, strictly controlling the JC-1 incubation time to within 10 minutes to effectively avoid probe toxicity damage to mitochondria; for hepatopancreas tissue, in vivo incubation is extended to 1 hour to ensure sufficient dye penetration. Under the condition of uniformly using 2 μM JC-1 and 20 μmol / L CCCP as positive controls, this method significantly improves the tissue penetration and signal uniformity of the dye, solving problems such as uneven staining and weak signal caused by exoskeleton barriers and high lipid content, providing reliable technical support for aquatic health assessment and toxicology research.

The invention discloses a method for marking strontium in enhanced and released crustacean yoke cardia bones, and relates to the field of fishery resources and environments. The method comprises the following steps: culturing the crustacean aquatic animals in a strontium-containing water body, and labeling the yoke cardia bones of the crustacean aquatic animals with strontium. According to the method, various potential target tissues of crustaceans are systematically screened, it is determined that the yoke cardia bone is the most suitable target organ marked by the strontium element for the first time, the enrichment effect of the strontium element is remarkable and stable, the marking characteristics are clear and easy to detect, and the problem that due to shell molting, a traditional marker is prone to falling off is effectively solved. Compared with a conventional method, the marking cost is greatly reduced, and the economical advantage is outstanding; and meanwhile, by virtue of the strong specificity of the element fingerprints, a release group and a natural group can be accurately distinguished, the core requirements of enhancement release on the stability and traceability of a marking technology can be met, and an efficient and reliable technical support is provided for release effect evaluation and population tracking management in crustacean wild resource restoration.

The invention relates to the field of food processing, in particular to a formula and a processing method of first-grade river-crossing fish. The processing method comprises the following steps: selecting main materials, preparing auxiliary materials, preparing seasonings and cooking, namely selecting an aquatic grass carp with the weight of about 800g, scaling the grass carp, removing gills, teeth and viscera, thoroughly cleaning a black film in the belly, cutting the cleaned grass carp, obliquely cutting the grass carp from the head to the tail with the width of about 1.5 cm, and cooking the grass carp with the width of about 1.5 cm; the method comprises the following steps: cutting grass carp into fish meat and fish maw, stopping at the main bone, uniformly smearing cornstarch in each piece of fish meat and fish maw of the cut grass carp, then lifting the fish tail, slightly shaking, removing excessive cornstarch, boiling water in a pot, putting 10g of ginger slices, then lifting the fish tail, putting the fish head into the water, cooking for 8-10 seconds, putting the whole grass carp into the pot, and cooking for 2-3 minutes with soft fire. According to the method, the aquatic grass carps with complete scales, full eyeballs, no whitening, no belly and high activity are selected, so that the freshness and quality of the raw materials are ensured, and a solid foundation is laid for making high-quality dishes.

The invention relates to a fish respiratory rate detection method based on YOLO segmentation and optical flow tracking, and belongs to the technical field of aquatic organism physiological status monitoring. The method comprises the steps of video data input and initialization, video preprocessing and frame rate dynamic calibration, gill segmentation by a YOLO model and target screening, gill region-of-interest extraction, optical flow feature point tracking and dynamic maintenance, gill motion displacement calculation and signal optimization, respiratory rate calculation and smooth optimization, and result output and visualization. Under complex scenes such as water wave interference and fish swimming, the respiratory rate calculation error is smaller than or equal to + / -2 BPM, the effective data rate is larger than or equal to 95%, and the method is superior to the prior art (for example, the patent CN120599516A) and can be applied to scenes such as aquaculture health early warning and aquatic organism physiological experiments.

The present application relates to the field of aquaculture technology, and particularly relates to a culture method of bigmouth bass gill explants and application. The present application provides a culture method of bigmouth bass gill explants, which uses explant culture solution to culture the gill explants of bigmouth bass. The explant culture solution is formed by adding fetal bovine serum, fish serum, trinity, D-galactose, L-alanyl-L-glutamine, sodium pyruvate and melatonin into a basic culture medium. In combination with dynamic culture conditions, the natural three-dimensional structure and intercellular connection of the gill tissue can be preserved, and the metabolic activity and functional integrity thereof can be maintained. The present application can solve the technical problem that the prior art cannot provide an ideal research model which can both maintain the structural integrity of the gill tissue and accurately reflect the functional characteristics of the gill tissue. Through the culture method, research models such as ammonia nitrogen stress can be efficiently constructed, thereby providing technical support for the research of bigmouth bass gill tissue physiological mechanism, disease prevention and control, environmental risk assessment and the like.

A device collects aquatic field metabolic rate data related to a water-breathing animal having at least one gill opening. The device has at least one oxygen probe, which measures and records oxygen consumption or oxygen extraction data at the at least one gill opening. The device is configured to collect metabolic data when the animal is in a natural environment. A method for determining a metabolic rate of the animal located in a water environment includes measuring metabolic rate data via at least one gill oxygen probe which is inserted into the at least one gill opening; measuring ambient data in the water environment via at least one ambient oxygen probe; and estimating a whole animal metabolic rate.

The invention relates to a river crab bean curd premade dish based on whole-course non-thermal processing and a making method thereof, and belongs to the technical field of aquatic product processing. A preparation method of a river crab bean curd premade dish based on whole-course non-thermal processing comprises the following steps: removing crab shells, gills, mouth umbilicus, heart and stomach and intestine of river crabs, crushing and filtering to obtain crab meat juice; and then adding the yeast extract and Arabic gum, stirring and mixing, homogenizing at high pressure, carrying out vacuum plastic packaging, sterilizing at ultrahigh pressure, and freezing to obtain the product. According to the method provided by the invention, through combination and synergy of a series of non-thermal processing technologies, the original flavor of the river crabs is reserved and improved to the greatest extent on the premise of ensuring the microbial safety of the product, and meanwhile, the characters of the final product are optimized, so that industrial production and long-term storage can be realized, and excellent quality can be kept.

To provide a method for using Antrodia cinnamomea essential oil for anesthetic treatment of spotted seabass, in which a problem to be solved is the feasibility of using Antrodia cinnamomea essential oil, which is a natural plant extract, as a new anesthetic to anesthetize live fish, and reduction in stress reaction and tissue damage in the process of anesthetizing live fish.SOLUTION: After spotted seabass is anesthetized by adding Antrodia cinnamomea essential oil in water, the morphological structure of the gill tissue of the spotted seabass and the influence on physiological, biochemical, and oxidative stress are studied, and the blood biochemical stress level, antioxidant enzyme activities, and immune indexes of the spotted seabass are measured to provide a theoretical basis for the anesthetic treatment of the sea fish. As compared with the expensive MS-222 and linalool, the price of Antrodia cinnamomea essential oil is 1 / 10 thereof, which can effectively reduce the cost of anesthesia, is particularly suitable for vaccination, blood collection, weighing, and transportation of live fish, etc., and has a higher economic benefit.SELECTED DRAWING: Figure 2

This invention belongs to the field of bio-aquaculture technology, specifically relating to SNP loci related to the body color of the leopard-gill spiny perch and their applications. This invention provides SNP loci (16_797851, 13_24322516, and 9_17302939) associated with the body color of the leopard-gill spiny perch. Furthermore, this invention provides a method for mining SNP loci and a method for enhancing the red color of the leopard-gill spiny perch's skin. By employing the HSV color model, the red body color is transformed from a direct sensory representation into a continuous quantitative variable, providing a data foundation for the genetic analysis of body color traits. Based on genome-wide molecular marker screening, genetic markers significantly associated with body color traits are obtained. Combined with the administration of agonists to individuals with genetic advantages, a significant enhancement of the red color of the leopard-gill spiny perch's body surface can be achieved. Therefore, the technical solution of this invention can not only directionally improve the body color trait of the leopard-gill spiny perch and cultivate high-quality new strains with stable body color inheritance, but also promote the development of marine aquaculture and the seed industry.

The utility model discloses a multi-layer high-density breeding device for plectropomus leopardus, which comprises a water drainage pipe vertically arranged in the center of a breeding pond and a plurality of inhabiting plates fixedly and vertically arranged on the water drainage pipe at intervals, and the inhabiting plate on the bottommost side is supported on the bottom wall of the breeding pond through a bottom layer supporting column. Every two adjacent inhabiting plates are connected through a supporting rod in a supporting mode, and the diameter of each layer of inhabiting plates is sequentially increased from top to bottom. According to the multi-layer high-density breeding device for the plectropomus leopardus, the rest area of the plectropomus leopardus is enlarged, the breeding density is improved and the breeding benefits are improved by utilizing the original facility drainage pipe in the breeding pond and adding more layers of inhabiting plates under the condition that the area of the breeding pond and the water body are not increased.

The present application belongs to the technical field of fish genetic breeding, and discloses a method for improving salt tolerance of grass carp, and specifically discloses a method for improving salt tolerance of fish, which comprises the following steps: taking fish fertilized eggs as initial domestication objects, and placing the fish fertilized eggs in salt water for hatching and domestication; thereafter, the salinity of the water body is increased by 1‰-2‰ every 25-35 days until the salinity is the same as that of the target aquaculture water body. The present application provides a footprint method based on salinity gradual domestication to improve the salt tolerance of fish. The method can effectively activate the expression of tissue osmotic pressure regulation related genes such as gills and kidneys, and ultimately can enable grass carp to adapt to 7‰ water body salinity, breaking the conventional salt tolerance threshold of grass carp. The main performance is that the grass carp still has the characteristics of rapid growth, healthy body, and strong feeding under 7‰ water body salinity.

The invention belongs to the technical field of fish genetic breeding, and discloses a method for improving salt tolerance of grass carps, in particular to a method for improving salt tolerance of fishes, which comprises the following steps: taking fish fertilized eggs as initial domestication objects, placing the fish fertilized eggs in saline water for incubation and domestication, then increasing the salinity of a water body by 0.1%-0.2% per 25-35 days, the salinity is the same as that of the target culture water body. The invention provides an imprinting method based on gradual salinity domestication to improve the salt tolerance of fishes, the method can effectively activate the expression of osmotic pressure regulation related genes of tissues such as gill and kidney, finally, the grass carp can adapt to 7 per mill of water salinity, the conventionally considered salt tolerance threshold value of the grass carp is broken through, and the application prospect is wide. The feed still has the characteristics of fast growth, healthy physique, strong ingestion and the like under the water salinity of 7 per mill.

The utility model belongs to the technical field of shrimp gill tissue crushing, and particularly relates to a quick crusher for gill tissues of penaeus vannamei boone, which comprises an adjusting base, an adjusting type container cooling mechanism is mounted at the top of the adjusting base, and the adjusting type container cooling mechanism comprises a plurality of cooling clamping plates. The multiple cooling clamping plates are annularly arranged and slidably mounted above the adjusting base, and circulating cooling channels are formed in the cooling clamping plates. A cooling liquid storage box is arranged on one side of the adjusting base, an electric lifting push rod is fixed to the top of the cooling liquid storage box, a supporting plate is fixed to the top of the electric lifting push rod, and a first motor is fixed to the top of one end of the supporting plate. According to the utility model, through the cooling system which can be moved and adjusted, by adjusting the position of the cooling system, the containers with different specifications can be attached and fixed, the low temperature in the containers can be maintained, the containers are convenient to disassemble and replace, the containers with proper capacities can be selected according to required sample sizes, and the use is more convenient.

This invention provides an integrated automatic processing device for crayfish, comprising: a frame, an inverted slide assembly, a Z-shaped cantilever, an adaptive clamping mechanism, a deveining mechanism, a head removal mechanism, and a back-opening and gill-removing mechanism. The inverted slide assembly is mounted on the frame. The lower end of the Z-shaped cantilever is fixedly connected to a slider. The adaptive clamping mechanism is mounted on the Z-shaped cantilever to fix the crayfish body. The deveining mechanism is coaxially arranged with the adaptive clamping mechanism. The head removal mechanism and the back-opening and gill-removing mechanism are sequentially arranged above the moving path. This invention achieves physical isolation between the transmission area and the processing area through the inverted slide and the Z-shaped cantilever, avoiding contamination and jamming. It employs a single-servo linkage type double-claw adaptive clamping, reducing the power source. Deveining is achieved through clamping and pulling; head removal uses an electric push rod-driven arc-shaped rotary cutter in conjunction with the slide's backward shearing; and back-opening and gill-removing uses a single motor-driven circular saw blade and a double-reverse steel wire brush, achieving integrated automatic processing of deveining, head removal, back-opening, and gill removal.

The invention relates to the technical field of aquaculture, in particular to a culture method and application of gill explants of micropterus salmoides. According to the technical scheme, the culture method of the gill explant of the largemouth bass comprises the following steps: culturing the gill explant of the largemouth bass by using an explant culture solution; the explant culture solution is formed by adding fetal calf serum, fish serum, three antibodies, D-galactose, L-alanyl-L-glutamine, sodium pyruvate and melatonin into a basic culture medium. In cooperation with dynamic culture conditions, the natural three-dimensional structure and intercellular connection of the gill tissue can be reserved, and the metabolic activity and functional integrity of the gill tissue can be maintained. According to the technical scheme, the technical problem that an ideal research model capable of maintaining the structural integrity of the gill tissue and accurately reflecting the functional characteristics of the gill tissue cannot be provided in the prior art can be solved. By means of the culture method, research models of ammonia nitrogen stress and the like can be efficiently constructed, and technical support is provided for micropterus salmoides gill tissue physiological mechanism research, disease prevention and control, environmental risk assessment and the like.

The invention discloses a steppe red oomycete strain, a microbial agent thereof and application of the steppe red oomycete strain in aquaculture, the steppe red oomycete strain is sent to China General Microbiological Culture Collection Center (CGMCC) for preservation, the preservation number is CGMCC No.35769, and the preservation date is August 28, 2025. The strain disclosed by the invention is subjected to anaerobic illumination culture in a conventional photosynthetic bacterium liquid culture medium to form a red viscous trait microbial agent, and the degradation rate of the microbial agent on H2S in water can reach 90% or above; meanwhile, the water purification effect is achieved when the fertilizer is applied to a culture pond with organic matter exceeding the standard, odor can be eliminated, and water can be cleared. The strain disclosed by the invention can effectively block toxic stress such as gill injury, hepatopancreas poisoning and the like of H2S to fish, shrimp and crab culture organisms and remarkably improve culture water environment and bottom mud quality, so that an efficient and environment-friendly novel microbial resource is provided for water H2S pollution treatment in aquaculture; the method is especially suitable for high-density pond culture and has wide practical application prospects and popularization value.

The invention discloses a multi-stage purification recirculating aquaculture system for plectropomus leopardus, and relates to the field of fish culture equipment. Comprising the steps that a culture pond body is designed in a partitioned mode to be matched with plectropomus leopardus to inhabit and swim, multi-stage purification treatment modules are arranged in sequence to guarantee clean water quality, a water quality real-time monitoring module collects parameters in the whole area and synchronizes the parameters to a control center, and an intelligent water circulation driving module achieves efficient water circulation through a variable frequency water pump and a pipeline. The dynamic regulation and control module adjusts breeding environment parameters based on monitoring data, the precise feeding module sets a feeding scheme according to feeding habits, the pollution discharge collection and treatment module achieves waste recycling, and the system monitoring and early warning module stores data in a classified mode and conducts early warning in a classified mode. Through multi-stage purification and intelligent regulation and control, the aquaculture water quality is stabilized, diseases are reduced, and the aquaculture survival rate and the product quality are improved; pollution is reduced through precise feeding and pollution discharge design, cost is saved through an energy consumption optimization module, waste recycling meets the environmental protection requirement, and large-scale efficient breeding is assisted.

This invention discloses a method for constructing and applying a marine fish muscle stem cell line. The culture medium uses basal medium as the background medium and contains LIF growth factor, EGF growth factor, and FGF2 protein derived from the leopard-gill sea bass. The final concentration of FGF2 protein from the leopard-gill sea bass is 20-30 ng / mL, 30-45 ng / mL, 45-55 ng / mL, or 55-70 ng / mL. Passaging using this medium can construct a stable, rapidly dividing, and short-passaging immortalized marine fish muscle stem cell line, capable of stable passage for more than 50 generations. This provides raw materials for exploring the function and gene regulation mechanisms of myoblasts, and provides in vitro verification experimental conditions for further research on the effects of marine fish muscle stem cells on growth rate, laying the foundation for the breeding of superior marine fish species.

The invention relates to the technical field of animal morphological research and specimen making, in particular to a microminiature fish skeleton specimen making method which comprises the following steps: step 1, standardized trapping and feeding of white skin beetles: preparing a feeding box and a worm-eaten box, trapping and feeding the white skin beetles, and screening out vigor larvae with the body length of more than or equal to 0.5 cm for later use; step 2, pretreatment of fish materials: selecting microminiature sclerobone fish with the body length of less than 10cm, freezing to death, sterilizing and dehydrating with 75% or more alcohol, and drying and shaping at the temperature of less than 65 DEG C after primary treatment of epidermis; according to the method, accurate fleshing is conducted on the larvae of the white skin beetles, damage to fine skeletons such as fin rays and gill bones is close to zero, the problem of structural damage of a traditional fleshing method is solved, the fine skeleton features of a specimen are clear and distinguishable, a standardized breeding system and an atomization bleaching process of the white skin beetles are established, the risk of skeleton disintegration of traditional soaking bleaching is avoided, and the method is suitable for large-scale popularization and application. And the manufacturing process is repeatable and small in error.

The utility model discloses a lure lure capable of generating water spray, which comprises a lure lure body, and water excitation cheek pieces extending outwards are arranged on cheek portions on two sides of the lure lure body. Compared with the prior art, the artificial bait has the advantages that the water excitation cheek pieces are arranged on the cheek portions on the two sides of the artificial bait body, and water can be generated through the water excitation cheek pieces to attract fishes. The fishhook guide hole and the fishhook hiding notch are formed in the head of the lure body, so that the lure body is prevented from being damaged due to repeated disassembly and assembly of the fishhook.

The invention discloses a self-adaptive freshwater fish scaling and sectioning all-in-one machine, and belongs to the technical field of aquatic product processing mechanical equipment. The all-in-one machine is sequentially provided with a scaling module, a conveyor belt self-adaptive centering conveying module, a gill removing module, a laparotomy module and a viscera removing module along a processing assembly line. The device is characterized in that the conveyor belt module adopts a symmetrical rocker arm connecting rod mechanism to realize self-adaptive adjustment of the clamping width; the belly cutting module is provided with a follow-up lifting mechanism and is matched with real-time fish belly contour detection to realize accurate cutting; the viscera removing module adopts a profiling brush wheel to clean the abdominal cavity; and the gill removing module realizes automatic gill removing. The equipment is high in integration level and strong in adaptability, and integrated automatic processing from scaling to viscera removing of various freshwater fishes is realized.