14 results about "Bacterial enteritis" patented technology

The invention relates to the technical field of microorganisms, and particularly discloses a metagen as well as a preparation method and anti-inflammatory application thereof. The method for preparing the metagen comprises the following steps: (1) carrying out solid-state fermentation by taking bacillus amyloliquefaciens CAU X1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.26769 and bacillus coagulans B2 with the preservation number of CGMCC No.26767 as zymophyte together, so as to obtain a fermentation product, wherein the bacillus amyloliquefaciens CAU X1 and the bacillus coagulans B2 are used as zymophyte together, and the bacillus amyloliquefaciens CAU X1 and the bacillus coagulans B2 are used as zymophyte together; (2) extracting the fermentation product by using a water-soluble solvent, centrifuging to take supernatant, and freeze-drying to obtain powder; or extracting the fermentation product by using an organic solvent, centrifuging and taking the supernatant, and volatilizing the organic solvent to obtain the extract. The metagen provided by the invention has anti-inflammatory activity, can improve bacterial enteritis, can play roles in resisting inflammation, regulating body immunity, optimizing intestinal flora structure and improving bacterial enteritis, can be used for medicines or feed additives, and has a good application prospect.

The present application relates to the technical field of pharmaceutical chemistry, and particularly relates to application of nitrothiazole derivatives and hydrochloride and sulfate thereof in preparation of medicines for treating intestinal infection of poultry and livestock. The nitrothiazole derivatives synthesized by the present application have high biological activity at the cell level, and have good biological activity on bacterial enteritis of poultry and livestock, especially chicken, pig, sheep and deer, and have high application value.

The present invention belongs to the fields of biotechnology and medical preparations, and provides yeast microcapsules delivering BBR / EGCG probiotic preparations and their applications. The present invention uses yeast membranes to encapsulate the probiotic Nissle 1917 and the anti-inflammatory and antioxidant natural products BBR and EGCG. This formulation exhibits good tolerability and cell targeting, facilitates intestinal colonization, and exerts anti-inflammatory and mucosal repair functions. It is particularly effective in treating enteritis caused by Salmonella typhimurium and has broad application prospects. This invention addresses the problem of the poor effectiveness of existing probiotics in treating bacterial enteritis.

The invention discloses a traditional Chinese medicine composition for preventing and treating fish enteritis. The traditional Chinese medicine composition is prepared from 10-50 parts of rheum officinale, 10-30 parts of origanum vulgare, 10-30 parts of herba andrographitis, 10-30 parts of humifuse euphorbia herb, 5-20 parts of radix aucklandiae and 5-20 parts of rhizoma smilacis glabrae. The corresponding preparation method comprises the following steps: stir-frying the rheum officinale with yellow wine, and stir-frying half of the radix aucklandiae with bran; all the components are weighed according to the formula and smashed to obtain traditional Chinese medicine composition powder, or all the components are weighed according to the formula, extracted and concentrated to be prepared into traditional Chinese medicine composition oral liquid, granules, extract powder or tablets. The invention relates to application in preparation of medicine-containing feed or medicine for preventing and treating fish enteritis. The traditional Chinese medicine composition has a good prevention and treatment effect on bacterial enteritis of fishes, can improve the immune function and disease resistance of the fishes, relieve inflammatory response caused by pathogenic infection, delay the morbidity process of fish enteritis caused by pathogenic bacteria and reduce the death rate of the fishes, and also can improve the feed intake of the fishes, reduce the feed coefficient and effectively improve the production performance.

The invention discloses a preparation method of eugenol-loaded quercetin-zinc nano-enzyme for treating enteritis of livestock. The nano-enzyme is constructed by forming nano-enzyme particles by virtue of coordination of quercetin and zinc ions and loading eugenol by virtue of metal-hydroxyl interaction, the nano-enzyme has the high-efficiency antioxidant activity of quercetin-zinc nano-enzyme and the anti-inflammatory and antibacterial functions of eugenol, and can synergistically inhibit pathogenic bacteria in intestinal tracts, remove excessive reactive oxygen species (ROS) at intestinal inflammation parts and promote the recovery of the intestinal tracts. The nano-enzyme is good in biocompatibility and high in stability, can slowly release active ingredients in an intestinal environment, is lasting in effect, can effectively relieve symptoms of bacterial enteritis and promote intestinal tissue repair, provides a novel efficient treatment strategy for treatment of bacterial enteritis, and has important clinical application value.

The application discloses application of fullerene in preparation of products for relieving bacterial enteritis of piglets, and belongs to the technical field of livestock and poultry breeding and feed preparation. It is found by experiment verification that the fullerene can relieve the bacterial enteritis of piglets: E. coli attack can significantly inhibit the growth performance of piglets after weaning and aggravate diarrhea, the addition of C60 in the daily ration can relieve the growth inhibition induced by K88 after weaning to a certain extent, and significantly reduce the post-weaning diarrhea rate and diarrhea index of piglets; the E. coli attack significantly up-regulates the mRNA expression amount of proinflammatory factors IL-1beta and IL-6 in the colon, and the daily ration added with C60 significantly inhibits the abnormal expression of the two proinflammatory factors; the E. coli attack leads to the significant increase of the contents of serum proinflammatory factors TNF-alpha and IL-6, and the addition of C60 in the daily ration significantly reduces the concentrations of TNF-alpha and IL-6 in the serum. It can be seen that the application provides a new solution for the bacterial enteritis of piglets.

The application provides a method for breeding golden pompano, which comprises the following steps: setting a net cage, selecting and releasing fry, bait enhancement and daily management, selecting a suitable sea area as the breeding environment of the net cage according to the living habit of the golden pompano, ensuring a healthy breeding environment, enhancing the bait according to three stages of the golden pompano with different ages, and adjusting the bait according to the growth of the fish body in each stage, so that the fish body reaches a micro-ecological balance in nutrition, immunity, growth stimulation and biological antagonism, and the reproduction of bacterial enteritis is inhibited; the fry is irradiated by visible light with a wavelength of 597-3400 nm while the net cage is cleaned, which can promote the release of cytokines on the surface of the fish body, prevent the infection of vibrio campbellii, tropical pseudomonas aeruginosa and gram-negative bacilli, and the survival rate of the method for breeding the golden pompano is 96.5-98.1%, the incidence of bacterial colitis is 0.3-1.2%, and the incidence of furuncle disease is 0.2-0.9%.

The invention belongs to the technical field of traditional Chinese medicines, and particularly discloses application of Chinese tamarisk twigs in preparation of a medicine for treating chicken bacterial enteritis. And side effects and potential problems caused by long-term use of antibiotics for treatment are effectively solved. A preparation method of the Chinese tamarisk twig extract comprises the following steps: taking a Chinese tamarisk twig medicinal material, adding 50% ethanol which is 10 times of the Chinese tamarisk twig medicinal material to soak for 1 hour, then performing ultrasonic extraction for 1 hour at 50 DEG C, and collecting an extracting solution; then 50% ethanol with the amount 8 times that of the medicinal materials is continuously added into the medicinal materials, ultrasonic extraction is conducted for 1 hour at the temperature of 50 DEG C, and an extracting solution is collected; combining the two extracting solutions, carrying out vacuum suction filtration, carrying out 50 DEG C rotary steam concentration to obtain a concentrated solution, and freeze-drying the concentrated solution to obtain a powdery Chinese tamarisk twig extract; the effective dose of the Chinese tamarisk twig extract is 1g / kg; the Chinese tamarisk twig extract is added into chicken feed as a feed additive. The invention is helpful for reducing the dependence on chemical synthetic drugs and the long-term sustainable development of animal breeding industry.

This invention relates to the fields of molecular biology and biomedicine, specifically to a method and application of recombinant methods for the grass carp IL-21 optimized gene, its recombinant protein, and plasmids. The recombinant protein of this invention is a mature grass carp IL-21 peptide. The pET-28a-IL-21 recombinant expression plasmid was constructed by optimizing the IL-21 base codons, transformed into an *E. coli* (DE3) expression system, and soluble grass carp IL-21 recombinant protein was obtained by IPTG induction. Furthermore, this invention also found that the optimized gene, its recombinant protein, and plasmids can enhance the intestinal barrier integrity of grass carp, inhibit the load of pathogenic bacteria in the intestine, and repair intestinal damage caused by bacterial infection. This indicates that the grass carp IL-21 recombinant protein has a significant effect on protecting intestinal homeostasis, highlighting its promising application prospects in the research and development of prevention and treatment of bacterial enteritis in aquatic animals.

The application discloses a kind of over rumen aureomycin premix for sheep digestive tract infection and preparation method, including capsule material and aureomycin premix, wherein, capsule material mass percentage is 45%-55%, and aureomycin premix mass accounts for 45%-55% of total mass.The premix of the application can avoid the degradation of aureomycin in sheep rumen, ensure sufficient active drug to reach intestinal lesions, and has excellent antibacterial and antidiarrheal effects on lamb dysentery, growing sheep stress bacterial enteritis and adult sheep digestive tract secondary bacterial infection, while reducing the dosage and reducing the risk of drug residues.

The invention belongs to the technical field of feed, and particularly relates to feed for resisting bacterial enteritis of pelteobagrus fulvidraco and application of the feed. By adding the corn protein enzymatic hydrolysate into the feed, the nutritional value of the feed can be remarkably improved, and breeding and feeding experiments prove that the corn protein enzymatic hydrolysate can effectively improve the growth performance of the pelteobagrus fulvidraco, promote the digestion and absorption efficiency of the pelteobagrus fulvidraco on protein, reduce nitrogen emission and improve the yield of the pelteobagrus fulvidraco. Therefore, the environmental pollution risk in the breeding process is reduced, and the disease resistance of the pelteobagrus fulvidraco is enhanced.

This invention discloses a nucleotide sequence targeting PepT1 to alleviate fish enteritis and its application. The nucleotide sequence includes a sense strand and an antisense strand, wherein the sequence of the sense strand is shown in SEQ ID NO. 6, and the sequence of the antisense strand is shown in SEQ ID NO. 7. When injected into fish, this nucleotide sequence targeting PepT1 to alleviate fish enteritis can effectively knock down the expression of the PepT1 gene in the fish intestine, with a knockdown efficiency of over 90%, demonstrating high knockdown efficiency. It can completely inhibit the expression level of the fish intestinal inflammatory factor IL-1β induced by bacterial MDP, significantly alleviating the intestinal inflammatory response in fish and exhibiting good therapeutic effects for fish enteritis. This provides new ideas for the research and preparation of drugs for the prevention and treatment of fish bacterial enteritis (such as fish immunomodulators and fish bacterial enteritis treatment drugs).

This invention discloses the application of β,β-dimethylacrylshikonin in the prevention and control of nonbacterial enteritis in carnivorous fish. Through experiments, the applicant discovered that, under the condition of replacing 50% of fishmeal protein in the feed with soybean meal protein, the addition of shikonin compounds, shikonin alcohol extract, or shikonin ultrafine powder can effectively improve the symptoms of enteritis in pearl grouper, increase the average weight gain rate of pearl grouper, improve the antioxidant capacity of pearl grouper, improve intestinal tissue structure, reduce the transcription levels of pro-inflammatory factors IL1β and IL8 in the intestine, and simultaneously upregulate the transcription level of anti-inflammatory factor IL10. Therefore, shikonin compounds, shikonin alcohol extract, or shikonin ultrafine powder have significant effects on soybean meal-induced enteritis in fish and can be used to prepare drugs or feed additives for the treatment or prevention of soybean meal-induced enteritis in fish.

The invention discloses a feed additive for preventing and treating bacterial enteritis of poultry and a preparation method of the feed additive, and relates to the technical field of feed additives. The feed additive comprises the following raw materials: benzoic acid, a coating material, a synergist and an essential oil component, the synergist is prepared by mixing malic acid and fumaric acid; the essential oil component is prepared by mixing thymol, eugenol, oregano essential oil, trans-anethole and piperine; the coating material is prepared from hydroxypropyl methyl cellulose, starch acetate and glyceryl monostearate. The preparation method of the feed additive comprises the steps of preliminary mixing, coating material preparation and finished product feed additive preparation. The prepared feed additive is good in benzoic acid slow-release effect, excellent in antibacterial ability and capable of effectively inhibiting bacterial enteritis of poultry.